ValRS-m cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ValRS-m
Entrez Gene ID	39023
Full Name	Valyl-tRNA synthetase, mitochondrial
Synonyms	CG5660,Dmel\CG5660
General protein information	Preferred Names Valyl-tRNA synthetase, mitochondrial Names CG5660 gene product from transcript CG5660-RB CG5660-PA CG5660-PB ValRS-m-PA ValRS-m-PB mitochondrial valyl-tRNA synthetase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 66E5-66E5

mRNA and Protein(s)

mRNA	Protein	Name
NM_140011.3	NP_648268.1	Valyl-tRNA synthetase, mitochondrial, isoform A
NM_001300059.1	NP_001286988.1	Valyl-tRNA synthetase, mitochondrial, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG5660	NP_648268.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006624	XP_316654.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ValRS-m gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ValRS-m cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa17962	NM_140011.3 Latest version!	Drosophila melanogaster Valyl-tRNA synthetase, mitochondrial (ValRS-m), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa17962	NM_001300059.1 Latest version!	Drosophila melanogaster Valyl-tRNA synthetase, mitochondrial (ValRS-m), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ValRS-m ( NM_140011.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648268.1 Drosophila melanogaster Valyl-tRNA synthetase, mitochondrial (ValRS-m), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa17962
Clone ID Related Accession (Same CDS sequence)	NM_140011.3 , NM_001300059.1
Accession Version	NM_140011.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Valyl-tRNA synthetase, mitochondrial, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140011.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAAATAA TTGGGAATTG TAGCAGTAAA CTGCTCAGAC ACAAGTACCT GTGCCAGCCA 
AGGGTGTACT CTCTATACTA CAGTACCCTT AAAGATGCGG CTAATGAGCT TCCCTTGGCA 
GCAGGATACC AGCCACAACC AGTGGAAAAT GCTTACTGGG AGCGGGAGCA TCGCCAGGCA 
AATTTGCCCA AGGCTTCAGC CAGCAGTTGC AAGCGGGGGA CCTATCGCAT GCTTCTACCG 
CCACCAAATG TAACCGGGAA TCTACATTTG GGCCATGCAC TGATGGCCAC CGTGCAGGAT 
GTGATTGCCC GCCAGCGCGA ACAGCTTGGC TACCAGGTGG ACTGGGTACC AGGCACAGAT 
CACGCGGGTA TCGCCACCCA GGTGGTCGTA GAGCGCACGA TTGCCGCTTC TCAGGCGAAA 
ACCCGTCATG AATTGGGACG TTCTGCTTTT CTGGACGAAG TATGGCGCTG GAAGGCGGAA 
AAGGGTGCTG GTATCGTACA GGATCTCCGC CAACTCGGCT GTAAACTGAA TTGGCAACGA 
GAATACTTCA CAATGGATGA GCAGCAGGCG CATGCGGTCA ACGTAGCTTT CGAGCGACTC 
TTTGAAGAAG GCCTAATTCA GCGGCGCAAC TCGGTTGTCA ACTGGTGCAC CGCTCTACGT 
TCCGCGATAT CTGATATCGA GGTGGACAGT GTAGAGATCA CGGAGCCGGT AGAGATATCC 
GTTCCAGGCT ACGATCACAA AGTACTCTTT GGCAGAATGT ACGATTTCGC CTATCACGTA 
GTGGATGGTG AAGCACTGCC CGATGGTTCG GTTGAAGAGA TTGTGGTGTC TACCACTAGA 
CCAGAGACAA TTTTGGGTGA TGTAGCTGTC GCAGTACATC CGCTGGATCC GCGCTACGCC 
AAGTACCGAA ACATTGATCA GGTTAAGCTC AAGCATCCTT TCCGTGACGA CACCATTCCA 
CTCGTTTTCG ATATCACCGT GGACCAGGAG TTTGGCACCG GGGCAGTGAA AATCACACCA 
GCCCATGACA AATTTGATTT CGAGCTGGCC ACTCGCCATA AGCTTGAACC TCGCCAGGTC 
TTTACTGAAA CGGGTCTTGT GGTGGATGCC TATTCTGAGT ACAAGGGTAT TCCGCGCTTC 
GAGGCGCGAG ATCTCATCGT CAATCGCCTG GAAGAGATGG ATTTGCTGCG ACAGGTGCGC 
TCTCATACCA TGCAGTTGCC CATCTGCTCG CGTTCCAAGG ACGTCATAGA GTACATGATA 
TTGCCGCAAT GGTTTCTTAA GTGCAAAGAC TTGGCAAAGG ATGCCTTATC TGAACTTCAT 
AGTGGACGTC TGCAAATCCT GCCTCCGAAC TTCGAAACGG AGTGGGAACG CTGGCTACAG 
GATAGCCGCG ACTGGTGCAT TTCCCGGCAG TTGTGGTGGG GGCATCAGGT GCCCGCATAC 
GAGGTCATCG ACTCCCAGGG AAACAGTCAA TGGGTGGCTG CCCTTGACGA GAAGACAGCT 
AGGCAGAAAG CCATGAGACT TATTGGTAGC GAAGAATTTA CATTGAAACG TGATCCGGAT 
GTACTGGACA CTTGGTTTTC ATCATCGCTG CTGCCTTTCT CCACAGCCGG TTGGCCAGAG 
GAGAGCTACA AAGAAAGATA TCCTTTGGAC ATAATGCAGA CTGGCCATGA TATTATCTTC 
TTTTGGGTGG CGCGCATGAT GATGTTGGGT CTGAAGCTGA CCGGCGAGGC ACCCTTTCAG 
AGAATTTTGC TAAACGGCAT TGTGTGCGAT GCTCATGGCC GGAAGATGTC CAAGAGTATC 
GGCAACATCG TGGCCCCACA GCAGGTGGTT CAGGGCGCCA GTTTAGAGAG CCTGAAGGCT 
GGCTTAGAGC AATCGTGCGA GGCGGGCATT ATTACGCCTT CAGAGTTAAA GGCCTCCACG 
ATTGGAATGA CGCAAATGTT CCCCAATGGC ATTCAAGAGT GCGGCACCGA TGCTCTACGC 
TTCACCCTGA TGAGCCACAA CATCAAGAGT CACTTCATTA GCTTCGATGT GAACGCCTGC 
CACACCAATA AGCTTTTCCT AAACAAAGTA TGGCAAGCTA TGCGATTTAC CCTCGGCTCA 
GCCAAGGGAC TGGGAATCTC GCTGCATCAG TTTGAGACTC TTGAAGGTGT GAATCTGGGC 
CTTTGGGATC GCTGGATCGT CGGACGCCTC GCGGAGACCT TGTCTGTCTG CTCGCAGAGC 
TACAGCAACT ACAATTTCCA CTTGGCCACA GCAGCCCTGA AGACCTTCTT CTATCAATAT 
CTCTGTGATA CTTACTTGGA AACAACCAAA CCTGCGATAG GAAATCGCAG TGCTGATGCC 
TACATCCATG TGGGCACCCT GACCGCTTGC CTTAGCTGGG GACTGCAGGC CATGGCCCCG 
TATGCTCCCT TTGTGGCTTC CGAATTACTG CAGCATGTGC CCCTTAATAT AGAGCTTAAA 
CTGTCCGACT ACAAGGATGA AAAGTTGGAG GAAGAGGTCA ACGAAATAGT GAACATCTGC 
CATAATGTGC GACAGGTTAA GAGTCGCAAT GAGATTAGCA AGCGCCATCA TCCGCAACTC 
AGTTTGTTTG CCCAGAATAC GGACTCGGAA GGAGTGCTTC GTCGTCATTT GCCGCAGATC 
AAGGTGTTAT CTCGCTGCGA AGACGTGGAA CTGGAATTGT TTGACGAGAG CTCAAAGATT 
TCGAAGCAAC TCAGCTACTT CTCAACGGCA GGAGCACTCT GCTCCTTTGG CTTGAAATTG 
AGCGATGGAT TGGATCTGAC GCCGGAAAAA CGAGACGAAA TGCAGAAGGC CAATGCCAAA 
AAACTGAAGA AGTTGGTCAC CGAATTGCAG CGGTATCGCA TGCGCCTGGA TAACGAAGCC 
TTCCAACTGA TGGCCGACAA GAAAGTTAAG TCACATTTCG AAAATAAGGT CAAAGAGTTG 
GAGGCGGAAA TCAACAGTCT CACTCGGCTG GCAGTATTGT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648268.1
CDS	38..3022
Translation	MQIIGNCSSKLLRHKYLCQPRVYSLYYSTLKDAANELPLAAGYQPQPVENAYWEREHRQANLPKASASSCKRGTYRMLLPPPNVTGNLHLGHALMATVQDVIARQREQLGYQVDWVPGTDHAGIATQVVVERTIAASQAKTRHELGRSAFLDEVWRWKAEKGAGIVQDLRQLGCKLNWQREYFTMDEQQAHAVNVAFERLFEEGLIQRRNSVVNWCTALRSAISDIEVDSVEITEPVEISVPGYDHKVLFGRMYDFAYHVVDGEALPDGSVEEIVVSTTRPETILGDVAVAVHPLDPRYAKYRNIDQVKLKHPFRDDTIPLVFDITVDQEFGTGAVKITPAHDKFDFELATRHKLEPRQVFTETGLVVDAYSEYKGIPRFEARDLIVNRLEEMDLLRQVRSHTMQLPICSRSKDVIEYMILPQWFLKCKDLAKDALSELHSGRLQILPPNFETEWERWLQDSRDWCISRQLWWGHQVPAYEVIDSQGNSQWVAALDEKTARQKAMRLIGSEEFTLKRDPDVLDTWFSSSLLPFSTAGWPEESYKERYPLDIMQTGHDIIFFWVARMMMLGLKLTGEAPFQRILLNGIVCDAHGRKMSKSIGNIVAPQQVVQGASLESLKAGLEQSCEAGIITPSELKASTIGMTQMFPNGIQECGTDALRFTLMSHNIKSHFISFDVNACHTNKLFLNKVWQAMRFTLGSAKGLGISLHQFETLEGVNLGLWDRWIVGRLAETLSVCSQSYSNYNFHLATAALKTFFYQYLCDTYLETTKPAIGNRSADAYIHVGTLTACLSWGLQAMAPYAPFVASELLQHVPLNIELKLSDYKDEKLEEEVNEIVNICHNVRQVKSRNEISKRHHPQLSLFAQNTDSEGVLRRHLPQIKVLSRCEDVELELFDESSKISKQLSYFSTAGALCSFGLKLSDGLDLTPEKRDEMQKANAKKLKKLVTELQRYRMRLDNEAFQLMADKKVKSHFENKVKELEAEINSLTRLAVLS

Target ORF information:

RefSeq Version	NM_140011.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Valyl-tRNA synthetase, mitochondrial (ValRS-m), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140011.3

ORF Insert Sequence:

ATGCAAATAA TTGGGAATTG TAGCAGTAAA CTGCTCAGAC ACAAGTACCT GTGCCAGCCA 
AGGGTGTACT CTCTATACTA CAGTACCCTT AAAGATGCGG CTAATGAGCT TCCCTTGGCA 
GCAGGATACC AGCCACAACC AGTGGAAAAT GCTTACTGGG AGCGGGAGCA TCGCCAGGCA 
AATTTGCCCA AGGCTTCAGC CAGCAGTTGC AAGCGGGGGA CCTATCGCAT GCTTCTACCG 
CCACCAAATG TAACCGGGAA TCTACATTTG GGCCATGCAC TGATGGCCAC CGTGCAGGAT 
GTGATTGCCC GCCAGCGCGA ACAGCTTGGC TACCAGGTGG ACTGGGTACC AGGCACAGAT 
CACGCGGGTA TCGCCACCCA GGTGGTCGTA GAGCGCACGA TTGCCGCTTC TCAGGCGAAA 
ACCCGTCATG AATTGGGACG TTCTGCTTTT CTGGACGAAG TATGGCGCTG GAAGGCGGAA 
AAGGGTGCTG GTATCGTACA GGATCTCCGC CAACTCGGCT GTAAACTGAA TTGGCAACGA 
GAATACTTCA CAATGGATGA GCAGCAGGCG CATGCGGTCA ACGTAGCTTT CGAGCGACTC 
TTTGAAGAAG GCCTAATTCA GCGGCGCAAC TCGGTTGTCA ACTGGTGCAC CGCTCTACGT 
TCCGCGATAT CTGATATCGA GGTGGACAGT GTAGAGATCA CGGAGCCGGT AGAGATATCC 
GTTCCAGGCT ACGATCACAA AGTACTCTTT GGCAGAATGT ACGATTTCGC CTATCACGTA 
GTGGATGGTG AAGCACTGCC CGATGGTTCG GTTGAAGAGA TTGTGGTGTC TACCACTAGA 
CCAGAGACAA TTTTGGGTGA TGTAGCTGTC GCAGTACATC CGCTGGATCC GCGCTACGCC 
AAGTACCGAA ACATTGATCA GGTTAAGCTC AAGCATCCTT TCCGTGACGA CACCATTCCA 
CTCGTTTTCG ATATCACCGT GGACCAGGAG TTTGGCACCG GGGCAGTGAA AATCACACCA 
GCCCATGACA AATTTGATTT CGAGCTGGCC ACTCGCCATA AGCTTGAACC TCGCCAGGTC 
TTTACTGAAA CGGGTCTTGT GGTGGATGCC TATTCTGAGT ACAAGGGTAT TCCGCGCTTC 
GAGGCGCGAG ATCTCATCGT CAATCGCCTG GAAGAGATGG ATTTGCTGCG ACAGGTGCGC 
TCTCATACCA TGCAGTTGCC CATCTGCTCG CGTTCCAAGG ACGTCATAGA GTACATGATA 
TTGCCGCAAT GGTTTCTTAA GTGCAAAGAC TTGGCAAAGG ATGCCTTATC TGAACTTCAT 
AGTGGACGTC TGCAAATCCT GCCTCCGAAC TTCGAAACGG AGTGGGAACG CTGGCTACAG 
GATAGCCGCG ACTGGTGCAT TTCCCGGCAG TTGTGGTGGG GGCATCAGGT GCCCGCATAC 
GAGGTCATCG ACTCCCAGGG AAACAGTCAA TGGGTGGCTG CCCTTGACGA GAAGACAGCT 
AGGCAGAAAG CCATGAGACT TATTGGTAGC GAAGAATTTA CATTGAAACG TGATCCGGAT 
GTACTGGACA CTTGGTTTTC ATCATCGCTG CTGCCTTTCT CCACAGCCGG TTGGCCAGAG 
GAGAGCTACA AAGAAAGATA TCCTTTGGAC ATAATGCAGA CTGGCCATGA TATTATCTTC 
TTTTGGGTGG CGCGCATGAT GATGTTGGGT CTGAAGCTGA CCGGCGAGGC ACCCTTTCAG 
AGAATTTTGC TAAACGGCAT TGTGTGCGAT GCTCATGGCC GGAAGATGTC CAAGAGTATC 
GGCAACATCG TGGCCCCACA GCAGGTGGTT CAGGGCGCCA GTTTAGAGAG CCTGAAGGCT 
GGCTTAGAGC AATCGTGCGA GGCGGGCATT ATTACGCCTT CAGAGTTAAA GGCCTCCACG 
ATTGGAATGA CGCAAATGTT CCCCAATGGC ATTCAAGAGT GCGGCACCGA TGCTCTACGC 
TTCACCCTGA TGAGCCACAA CATCAAGAGT CACTTCATTA GCTTCGATGT GAACGCCTGC 
CACACCAATA AGCTTTTCCT AAACAAAGTA TGGCAAGCTA TGCGATTTAC CCTCGGCTCA 
GCCAAGGGAC TGGGAATCTC GCTGCATCAG TTTGAGACTC TTGAAGGTGT GAATCTGGGC 
CTTTGGGATC GCTGGATCGT CGGACGCCTC GCGGAGACCT TGTCTGTCTG CTCGCAGAGC 
TACAGCAACT ACAATTTCCA CTTGGCCACA GCAGCCCTGA AGACCTTCTT CTATCAATAT 
CTCTGTGATA CTTACTTGGA AACAACCAAA CCTGCGATAG GAAATCGCAG TGCTGATGCC 
TACATCCATG TGGGCACCCT GACCGCTTGC CTTAGCTGGG GACTGCAGGC CATGGCCCCG 
TATGCTCCCT TTGTGGCTTC CGAATTACTG CAGCATGTGC CCCTTAATAT AGAGCTTAAA 
CTGTCCGACT ACAAGGATGA AAAGTTGGAG GAAGAGGTCA ACGAAATAGT GAACATCTGC 
CATAATGTGC GACAGGTTAA GAGTCGCAAT GAGATTAGCA AGCGCCATCA TCCGCAACTC 
AGTTTGTTTG CCCAGAATAC GGACTCGGAA GGAGTGCTTC GTCGTCATTT GCCGCAGATC 
AAGGTGTTAT CTCGCTGCGA AGACGTGGAA CTGGAATTGT TTGACGAGAG CTCAAAGATT 
TCGAAGCAAC TCAGCTACTT CTCAACGGCA GGAGCACTCT GCTCCTTTGG CTTGAAATTG 
AGCGATGGAT TGGATCTGAC GCCGGAAAAA CGAGACGAAA TGCAGAAGGC CAATGCCAAA 
AAACTGAAGA AGTTGGTCAC CGAATTGCAG CGGTATCGCA TGCGCCTGGA TAACGAAGCC 
TTCCAACTGA TGGCCGACAA GAAAGTTAAG TCACATTTCG AAAATAAGGT CAAAGAGTTG 
GAGGCGGAAA TCAACAGTCT CACTCGGCTG GCAGTATTGT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ValRS-m ( NM_001300059.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286988.1 Drosophila melanogaster Valyl-tRNA synthetase, mitochondrial (ValRS-m), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa17962
Clone ID Related Accession (Same CDS sequence)	NM_140011.3 , NM_001300059.1
Accession Version	NM_001300059.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Valyl-tRNA synthetase, mitochondrial, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAAATAA TTGGGAATTG TAGCAGTAAA CTGCTCAGAC ACAAGTACCT GTGCCAGCCA 
AGGGTGTACT CTCTATACTA CAGTACCCTT AAAGATGCGG CTAATGAGCT TCCCTTGGCA 
GCAGGATACC AGCCACAACC AGTGGAAAAT GCTTACTGGG AGCGGGAGCA TCGCCAGGCA 
AATTTGCCCA AGGCTTCAGC CAGCAGTTGC AAGCGGGGGA CCTATCGCAT GCTTCTACCG 
CCACCAAATG TAACCGGGAA TCTACATTTG GGCCATGCAC TGATGGCCAC CGTGCAGGAT 
GTGATTGCCC GCCAGCGCGA ACAGCTTGGC TACCAGGTGG ACTGGGTACC AGGCACAGAT 
CACGCGGGTA TCGCCACCCA GGTGGTCGTA GAGCGCACGA TTGCCGCTTC TCAGGCGAAA 
ACCCGTCATG AATTGGGACG TTCTGCTTTT CTGGACGAAG TATGGCGCTG GAAGGCGGAA 
AAGGGTGCTG GTATCGTACA GGATCTCCGC CAACTCGGCT GTAAACTGAA TTGGCAACGA 
GAATACTTCA CAATGGATGA GCAGCAGGCG CATGCGGTCA ACGTAGCTTT CGAGCGACTC 
TTTGAAGAAG GCCTAATTCA GCGGCGCAAC TCGGTTGTCA ACTGGTGCAC CGCTCTACGT 
TCCGCGATAT CTGATATCGA GGTGGACAGT GTAGAGATCA CGGAGCCGGT AGAGATATCC 
GTTCCAGGCT ACGATCACAA AGTACTCTTT GGCAGAATGT ACGATTTCGC CTATCACGTA 
GTGGATGGTG AAGCACTGCC CGATGGTTCG GTTGAAGAGA TTGTGGTGTC TACCACTAGA 
CCAGAGACAA TTTTGGGTGA TGTAGCTGTC GCAGTACATC CGCTGGATCC GCGCTACGCC 
AAGTACCGAA ACATTGATCA GGTTAAGCTC AAGCATCCTT TCCGTGACGA CACCATTCCA 
CTCGTTTTCG ATATCACCGT GGACCAGGAG TTTGGCACCG GGGCAGTGAA AATCACACCA 
GCCCATGACA AATTTGATTT CGAGCTGGCC ACTCGCCATA AGCTTGAACC TCGCCAGGTC 
TTTACTGAAA CGGGTCTTGT GGTGGATGCC TATTCTGAGT ACAAGGGTAT TCCGCGCTTC 
GAGGCGCGAG ATCTCATCGT CAATCGCCTG GAAGAGATGG ATTTGCTGCG ACAGGTGCGC 
TCTCATACCA TGCAGTTGCC CATCTGCTCG CGTTCCAAGG ACGTCATAGA GTACATGATA 
TTGCCGCAAT GGTTTCTTAA GTGCAAAGAC TTGGCAAAGG ATGCCTTATC TGAACTTCAT 
AGTGGACGTC TGCAAATCCT GCCTCCGAAC TTCGAAACGG AGTGGGAACG CTGGCTACAG 
GATAGCCGCG ACTGGTGCAT TTCCCGGCAG TTGTGGTGGG GGCATCAGGT GCCCGCATAC 
GAGGTCATCG ACTCCCAGGG AAACAGTCAA TGGGTGGCTG CCCTTGACGA GAAGACAGCT 
AGGCAGAAAG CCATGAGACT TATTGGTAGC GAAGAATTTA CATTGAAACG TGATCCGGAT 
GTACTGGACA CTTGGTTTTC ATCATCGCTG CTGCCTTTCT CCACAGCCGG TTGGCCAGAG 
GAGAGCTACA AAGAAAGATA TCCTTTGGAC ATAATGCAGA CTGGCCATGA TATTATCTTC 
TTTTGGGTGG CGCGCATGAT GATGTTGGGT CTGAAGCTGA CCGGCGAGGC ACCCTTTCAG 
AGAATTTTGC TAAACGGCAT TGTGTGCGAT GCTCATGGCC GGAAGATGTC CAAGAGTATC 
GGCAACATCG TGGCCCCACA GCAGGTGGTT CAGGGCGCCA GTTTAGAGAG CCTGAAGGCT 
GGCTTAGAGC AATCGTGCGA GGCGGGCATT ATTACGCCTT CAGAGTTAAA GGCCTCCACG 
ATTGGAATGA CGCAAATGTT CCCCAATGGC ATTCAAGAGT GCGGCACCGA TGCTCTACGC 
TTCACCCTGA TGAGCCACAA CATCAAGAGT CACTTCATTA GCTTCGATGT GAACGCCTGC 
CACACCAATA AGCTTTTCCT AAACAAAGTA TGGCAAGCTA TGCGATTTAC CCTCGGCTCA 
GCCAAGGGAC TGGGAATCTC GCTGCATCAG TTTGAGACTC TTGAAGGTGT GAATCTGGGC 
CTTTGGGATC GCTGGATCGT CGGACGCCTC GCGGAGACCT TGTCTGTCTG CTCGCAGAGC 
TACAGCAACT ACAATTTCCA CTTGGCCACA GCAGCCCTGA AGACCTTCTT CTATCAATAT 
CTCTGTGATA CTTACTTGGA AACAACCAAA CCTGCGATAG GAAATCGCAG TGCTGATGCC 
TACATCCATG TGGGCACCCT GACCGCTTGC CTTAGCTGGG GACTGCAGGC CATGGCCCCG 
TATGCTCCCT TTGTGGCTTC CGAATTACTG CAGCATGTGC CCCTTAATAT AGAGCTTAAA 
CTGTCCGACT ACAAGGATGA AAAGTTGGAG GAAGAGGTCA ACGAAATAGT GAACATCTGC 
CATAATGTGC GACAGGTTAA GAGTCGCAAT GAGATTAGCA AGCGCCATCA TCCGCAACTC 
AGTTTGTTTG CCCAGAATAC GGACTCGGAA GGAGTGCTTC GTCGTCATTT GCCGCAGATC 
AAGGTGTTAT CTCGCTGCGA AGACGTGGAA CTGGAATTGT TTGACGAGAG CTCAAAGATT 
TCGAAGCAAC TCAGCTACTT CTCAACGGCA GGAGCACTCT GCTCCTTTGG CTTGAAATTG 
AGCGATGGAT TGGATCTGAC GCCGGAAAAA CGAGACGAAA TGCAGAAGGC CAATGCCAAA 
AAACTGAAGA AGTTGGTCAC CGAATTGCAG CGGTATCGCA TGCGCCTGGA TAACGAAGCC 
TTCCAACTGA TGGCCGACAA GAAAGTTAAG TCACATTTCG AAAATAAGGT CAAAGAGTTG 
GAGGCGGAAA TCAACAGTCT CACTCGGCTG GCAGTATTGT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286988.1
CDS	144..3128
Translation	MQIIGNCSSKLLRHKYLCQPRVYSLYYSTLKDAANELPLAAGYQPQPVENAYWEREHRQANLPKASASSCKRGTYRMLLPPPNVTGNLHLGHALMATVQDVIARQREQLGYQVDWVPGTDHAGIATQVVVERTIAASQAKTRHELGRSAFLDEVWRWKAEKGAGIVQDLRQLGCKLNWQREYFTMDEQQAHAVNVAFERLFEEGLIQRRNSVVNWCTALRSAISDIEVDSVEITEPVEISVPGYDHKVLFGRMYDFAYHVVDGEALPDGSVEEIVVSTTRPETILGDVAVAVHPLDPRYAKYRNIDQVKLKHPFRDDTIPLVFDITVDQEFGTGAVKITPAHDKFDFELATRHKLEPRQVFTETGLVVDAYSEYKGIPRFEARDLIVNRLEEMDLLRQVRSHTMQLPICSRSKDVIEYMILPQWFLKCKDLAKDALSELHSGRLQILPPNFETEWERWLQDSRDWCISRQLWWGHQVPAYEVIDSQGNSQWVAALDEKTARQKAMRLIGSEEFTLKRDPDVLDTWFSSSLLPFSTAGWPEESYKERYPLDIMQTGHDIIFFWVARMMMLGLKLTGEAPFQRILLNGIVCDAHGRKMSKSIGNIVAPQQVVQGASLESLKAGLEQSCEAGIITPSELKASTIGMTQMFPNGIQECGTDALRFTLMSHNIKSHFISFDVNACHTNKLFLNKVWQAMRFTLGSAKGLGISLHQFETLEGVNLGLWDRWIVGRLAETLSVCSQSYSNYNFHLATAALKTFFYQYLCDTYLETTKPAIGNRSADAYIHVGTLTACLSWGLQAMAPYAPFVASELLQHVPLNIELKLSDYKDEKLEEEVNEIVNICHNVRQVKSRNEISKRHHPQLSLFAQNTDSEGVLRRHLPQIKVLSRCEDVELELFDESSKISKQLSYFSTAGALCSFGLKLSDGLDLTPEKRDEMQKANAKKLKKLVTELQRYRMRLDNEAFQLMADKKVKSHFENKVKELEAEINSLTRLAVLS

Target ORF information:

RefSeq Version	NM_001300059.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Valyl-tRNA synthetase, mitochondrial (ValRS-m), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300059.1

ORF Insert Sequence:

ATGCAAATAA TTGGGAATTG TAGCAGTAAA CTGCTCAGAC ACAAGTACCT GTGCCAGCCA 
AGGGTGTACT CTCTATACTA CAGTACCCTT AAAGATGCGG CTAATGAGCT TCCCTTGGCA 
GCAGGATACC AGCCACAACC AGTGGAAAAT GCTTACTGGG AGCGGGAGCA TCGCCAGGCA 
AATTTGCCCA AGGCTTCAGC CAGCAGTTGC AAGCGGGGGA CCTATCGCAT GCTTCTACCG 
CCACCAAATG TAACCGGGAA TCTACATTTG GGCCATGCAC TGATGGCCAC CGTGCAGGAT 
GTGATTGCCC GCCAGCGCGA ACAGCTTGGC TACCAGGTGG ACTGGGTACC AGGCACAGAT 
CACGCGGGTA TCGCCACCCA GGTGGTCGTA GAGCGCACGA TTGCCGCTTC TCAGGCGAAA 
ACCCGTCATG AATTGGGACG TTCTGCTTTT CTGGACGAAG TATGGCGCTG GAAGGCGGAA 
AAGGGTGCTG GTATCGTACA GGATCTCCGC CAACTCGGCT GTAAACTGAA TTGGCAACGA 
GAATACTTCA CAATGGATGA GCAGCAGGCG CATGCGGTCA ACGTAGCTTT CGAGCGACTC 
TTTGAAGAAG GCCTAATTCA GCGGCGCAAC TCGGTTGTCA ACTGGTGCAC CGCTCTACGT 
TCCGCGATAT CTGATATCGA GGTGGACAGT GTAGAGATCA CGGAGCCGGT AGAGATATCC 
GTTCCAGGCT ACGATCACAA AGTACTCTTT GGCAGAATGT ACGATTTCGC CTATCACGTA 
GTGGATGGTG AAGCACTGCC CGATGGTTCG GTTGAAGAGA TTGTGGTGTC TACCACTAGA 
CCAGAGACAA TTTTGGGTGA TGTAGCTGTC GCAGTACATC CGCTGGATCC GCGCTACGCC 
AAGTACCGAA ACATTGATCA GGTTAAGCTC AAGCATCCTT TCCGTGACGA CACCATTCCA 
CTCGTTTTCG ATATCACCGT GGACCAGGAG TTTGGCACCG GGGCAGTGAA AATCACACCA 
GCCCATGACA AATTTGATTT CGAGCTGGCC ACTCGCCATA AGCTTGAACC TCGCCAGGTC 
TTTACTGAAA CGGGTCTTGT GGTGGATGCC TATTCTGAGT ACAAGGGTAT TCCGCGCTTC 
GAGGCGCGAG ATCTCATCGT CAATCGCCTG GAAGAGATGG ATTTGCTGCG ACAGGTGCGC 
TCTCATACCA TGCAGTTGCC CATCTGCTCG CGTTCCAAGG ACGTCATAGA GTACATGATA 
TTGCCGCAAT GGTTTCTTAA GTGCAAAGAC TTGGCAAAGG ATGCCTTATC TGAACTTCAT 
AGTGGACGTC TGCAAATCCT GCCTCCGAAC TTCGAAACGG AGTGGGAACG CTGGCTACAG 
GATAGCCGCG ACTGGTGCAT TTCCCGGCAG TTGTGGTGGG GGCATCAGGT GCCCGCATAC 
GAGGTCATCG ACTCCCAGGG AAACAGTCAA TGGGTGGCTG CCCTTGACGA GAAGACAGCT 
AGGCAGAAAG CCATGAGACT TATTGGTAGC GAAGAATTTA CATTGAAACG TGATCCGGAT 
GTACTGGACA CTTGGTTTTC ATCATCGCTG CTGCCTTTCT CCACAGCCGG TTGGCCAGAG 
GAGAGCTACA AAGAAAGATA TCCTTTGGAC ATAATGCAGA CTGGCCATGA TATTATCTTC 
TTTTGGGTGG CGCGCATGAT GATGTTGGGT CTGAAGCTGA CCGGCGAGGC ACCCTTTCAG 
AGAATTTTGC TAAACGGCAT TGTGTGCGAT GCTCATGGCC GGAAGATGTC CAAGAGTATC 
GGCAACATCG TGGCCCCACA GCAGGTGGTT CAGGGCGCCA GTTTAGAGAG CCTGAAGGCT 
GGCTTAGAGC AATCGTGCGA GGCGGGCATT ATTACGCCTT CAGAGTTAAA GGCCTCCACG 
ATTGGAATGA CGCAAATGTT CCCCAATGGC ATTCAAGAGT GCGGCACCGA TGCTCTACGC 
TTCACCCTGA TGAGCCACAA CATCAAGAGT CACTTCATTA GCTTCGATGT GAACGCCTGC 
CACACCAATA AGCTTTTCCT AAACAAAGTA TGGCAAGCTA TGCGATTTAC CCTCGGCTCA 
GCCAAGGGAC TGGGAATCTC GCTGCATCAG TTTGAGACTC TTGAAGGTGT GAATCTGGGC 
CTTTGGGATC GCTGGATCGT CGGACGCCTC GCGGAGACCT TGTCTGTCTG CTCGCAGAGC 
TACAGCAACT ACAATTTCCA CTTGGCCACA GCAGCCCTGA AGACCTTCTT CTATCAATAT 
CTCTGTGATA CTTACTTGGA AACAACCAAA CCTGCGATAG GAAATCGCAG TGCTGATGCC 
TACATCCATG TGGGCACCCT GACCGCTTGC CTTAGCTGGG GACTGCAGGC CATGGCCCCG 
TATGCTCCCT TTGTGGCTTC CGAATTACTG CAGCATGTGC CCCTTAATAT AGAGCTTAAA 
CTGTCCGACT ACAAGGATGA AAAGTTGGAG GAAGAGGTCA ACGAAATAGT GAACATCTGC 
CATAATGTGC GACAGGTTAA GAGTCGCAAT GAGATTAGCA AGCGCCATCA TCCGCAACTC 
AGTTTGTTTG CCCAGAATAC GGACTCGGAA GGAGTGCTTC GTCGTCATTT GCCGCAGATC 
AAGGTGTTAT CTCGCTGCGA AGACGTGGAA CTGGAATTGT TTGACGAGAG CTCAAAGATT 
TCGAAGCAAC TCAGCTACTT CTCAACGGCA GGAGCACTCT GCTCCTTTGG CTTGAAATTG 
AGCGATGGAT TGGATCTGAC GCCGGAAAAA CGAGACGAAA TGCAGAAGGC CAATGCCAAA 
AAACTGAAGA AGTTGGTCAC CGAATTGCAG CGGTATCGCA TGCGCCTGGA TAACGAAGCC 
TTCCAACTGA TGGCCGACAA GAAAGTTAAG TCACATTTCG AAAATAAGGT CAAAGAGTTG 
GAGGCGGAAA TCAACAGTCT CACTCGGCTG GCAGTATTGT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ValRS-m cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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