CTCF cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CTCF
Entrez Gene ID	38817
Full Name	CG8591 gene product from transcript CG8591-RA
Synonyms	CG8591,Dmel\CG8591,dCTCF
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 65F6-65F6

mRNA and Protein(s)

mRNA	Protein	Name
NM_139852.4	NP_648109.1	CTCF

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mutation of the Mcp Pita site disrupts blocking activity and also eliminates dCTCF binding. In contrast, mutation of the Mcp dCTCF site does not affect Pita binding, and this mutant boundary retains partial function.
Title: Architectural protein Pita cooperates with dCTCF in organization of functional boundaries in Bithorax complex.

Drosophila CTCF-CP190 interactions are less critical for the in vivo functions of the CTCF protein than the N-terminal CTCF - CTCF interaction domain.
Title: Functional role of dimerization and CP190 interacting domains of CTCF protein in Drosophila melanogaster.

TCF participates in the recruitment of Smad proteins to a subset of genomic sites and in the redistribution of these proteins in response to BMP signaling.
Title: CTCF-dependent co-localization of canonical Smad signaling factors at architectural protein binding sites in D. melanogaster.

Evolutionarily conserved ENY2 is responsible for barrier activity mediated by the dCTCF protein.
Title: Highly conserved ENY2/Sus1 protein binds to Drosophila CTCF and is required for barrier activity.

Results show that CP190 binds to CTCF or to other DNA binding insulator factors serving as a binding platform for compexes with enzymatic function, like NURF and dREAM.
Title: A functional insulator screen identifies NURF and dREAM components to be required for enhancer-blocking.

The following CTCF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CTCF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa20637	NM_139852.4 Latest version!	Drosophila melanogaster CTCF (CTCF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CTCF ( NM_139852.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648109.1 Drosophila melanogaster CTCF (CTCF), mRNA.

Price & Availability↑

CloneID	OFa20637
Clone ID Related Accession (Same CDS sequence)	NM_139852.4
Accession Version	NM_139852.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	CTCF
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_139852.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCAAGGA GGACAAAAAA GGACGAGGAC CCCGAGGATC TGCAGACCTT CCTCAACAAT 
TTCCATAAGG AGATCGAAGG CAACAGCGAT GAGAAGGTGG TAAACACGAT TTTGGAGGCC 
ATCAGCGCCG AGGCCATCGA CTTGGATGAA AATGGTGCGG AGGCTGGTGG ATCGAAACCG 
ATGGAGGAGG CGGAGGCTGA TTTGGATCAT GCGGAGGAAG CGGAGGAGGA GGAGGAGGAC 
GACGAGGATA AGTATTTCAT AGATGACGAG GGCAACTGCT ATATCAAGAC TACGCCCAAG 
AAGCAAAAGG AGCTACAAAA GAAGCTCAAA CAGGCGGCTG CCAAGCCGGG GAAAGCTACA 
CGTTCCGTGG TCAGTACAGC CACCAATAAA TCCATCAATT TGCGGCCAGC CAAATCGACA 
CCGAAAGCGA CTACTTCGAA GCCTCCCCCA GAACCCAAGG CAATTAGCGT TCGACCAGCC 
CGAGCAGCTG CAGCCAAGGC GAAGCAATCG GCGATGCCAC CGCCACCGGC ATTAGTGGTC 
AAAGTACCGG CACCCAGGGG CAGGCCTAGA AAGAACCCTG TCATACCGAA GCCTGAACCC 
ATGGACTTGG AGCGGGAGCT CGAGGAGCTG GTAGATGAGC CCGACATCAG TTCAATGGTG 
ACAGAATTGT CGGACTATAC CGTAGACGAA GCTGCCGTAG AGGCAGCAAC AGCTACGCTG 
ACGCCAAACG AGGCTGAGGT CTACGAATTT GAGGACAATG CGACGACAGA GGATGAGAAT 
GCCGACAAGA AGGACGTAGA CTTTGTGCTA AGCAACAAGG AGGTGAAGCT GAAGACAGCT 
TCTTCTACCA GCCAGAATTC GAATGCATCG GGACATAAGT ACTCATGCCC ACACTGCCCG 
TATACGGCGA GCAAGAAGTT TCTGATCACG CGCCACTCAC GAAGTCATGA CGTCGAGCCA 
TCCTTCAAGT GCTCCATCTG CGAGAGGTCC TTTCGATCGA ACGTGGGCCT GCAAAACCAC 
ATAAACACGC ACATGGGCAA TAAGCCGCAC AAGTGCAAGC TCTGCGAAAG CGCCTTCACC 
ACCAGCGGCG AGCTGGTCCG TCACACTCGC TACAAGCACA CTAAGGAAAA ACCGCACAAG 
TGCACGGAGT GCACATACGC CAGCGTGGAA CTGACCAAAC TGCGCCGCCA CATGACCTGC 
CATACGGGCG AGAGACCATA TCAATGTCCG CACTGCACCT ACGCATCGCA GGACATGTTC 
AAGCTGAAGC GACACATGGT TATCCACACC GGCGAGAAGA AGTACCAATG CGACATCTGC 
AAGTCGCGAT TTACGCAATC TAACTCACTA AAGGCCCACA AGCTCATCCA CAGCGTGGTC 
GACAAGCCCG TCTTTCAGTG CAACTACTGC CCGACGACTT GCGGTCGGAA GGCTGACCTG 
CGCGTTCACA TTAAACACAT GCACACCTCT GATGTGCCAA TGACATGCCG GCGCTGTGGC 
CAGCAGCTGC CCGATCGCTA TCAATACAAG CTGCACGTTA AGTCACACGA GGGCGAAAAG 
TGTTACAGCT GCAAGCTGTG CAGCTACGCT TCGGTCACCC AACGCCATTT GGCCTCACAC 
ATGCTCATCC ACCTGGACGA AAAGCCGTTC CATTGCGATC AGTGCCCGCA GGCCTTCCGG 
CAGCGTCAAT TGCTGCGCCG CCATATGAAT CTCGTGCACA ACGAAGAGTA TCAGCCACCA 
GAACCGCGCG AGAAACTGCA CAAGTGTCCG AGTTGTCCGC GGGAGTTTAC CCACAAGGGT 
AATCTAATGA GACACATGGA GACTCACGAT GATTCGGCGA ATGCTCGCGA AAAGCGGCGT 
CGCCTTAAAT TGGGCCGCAA TGTGCGGCTG CAGAAAGATG GCACTGTCAT CACGTTGATC 
AAGGATCAGT ATGTGGATAT GGACCGGGAC CAGGAGGAAA ACGAAGAGGA TGACAACCCA 
GAGAGCTATG ACTTGGCTGA AATCGAGCCG GAGAACTCGG AGGCTGAAGA CGCCGATGAT 
GATGTGGAAA CCATAGTCAG TGATCCGATC CGTCAGAGAA TCAAGCCAGC ACCTATCATT 
ATCAACAAAC AGGCCAGGCT TGCCGCCTCC GAAAAGCAGC CGATGATCAT CAATCAACGC 
CTCCGAAGTC AACGGGGCAC CAAGACGTTC CACATCAAGG AGGAGCCGGA CAACTCGGAC 
TTCACCGTGG AATGGCAAGG GGATGATGGC GAGGTAATGG TCGTAGAGCT GGTTAATGGC 
GACGAGGAGG TGTTGGTCAA GCACGAACCG AGCGCCAACT CCAAGATCAG TGCCAAAAAC 
TGCTTTGGTT TCGAGGACGA CGATGATTAT GAGGAGTATG GCGATGGGGA AAACGAGGTC 
GATGGCGCTT CCCAGGAGTT TCTGCAGCTG ATGGATATGA TTGAGCAGGA CTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648109.1
CDS	136..2592
Translation	MPRRTKKDEDPEDLQTFLNNFHKEIEGNSDEKVVNTILEAISAEAIDLDENGAEAGGSKPMEEAEADLDHAEEAEEEEEDDEDKYFIDDEGNCYIKTTPKKQKELQKKLKQAAAKPGKATRSVVSTATNKSINLRPAKSTPKATTSKPPPEPKAISVRPARAAAAKAKQSAMPPPPALVVKVPAPRGRPRKNPVIPKPEPMDLERELEELVDEPDISSMVTELSDYTVDEAAVEAATATLTPNEAEVYEFEDNATTEDENADKKDVDFVLSNKEVKLKTASSTSQNSNASGHKYSCPHCPYTASKKFLITRHSRSHDVEPSFKCSICERSFRSNVGLQNHINTHMGNKPHKCKLCESAFTTSGELVRHTRYKHTKEKPHKCTECTYASVELTKLRRHMTCHTGERPYQCPHCTYASQDMFKLKRHMVIHTGEKKYQCDICKSRFTQSNSLKAHKLIHSVVDKPVFQCNYCPTTCGRKADLRVHIKHMHTSDVPMTCRRCGQQLPDRYQYKLHVKSHEGEKCYSCKLCSYASVTQRHLASHMLIHLDEKPFHCDQCPQAFRQRQLLRRHMNLVHNEEYQPPEPREKLHKCPSCPREFTHKGNLMRHMETHDDSANAREKRRRLKLGRNVRLQKDGTVITLIKDQYVDMDRDQEENEEDDNPESYDLAEIEPENSEAEDADDDVETIVSDPIRQRIKPAPIIINKQARLAASEKQPMIINQRLRSQRGTKTFHIKEEPDNSDFTVEWQGDDGEVMVVELVNGDEEVLVKHEPSANSKISAKNCFGFEDDDDYEEYGDGENEVDGASQEFLQLMDMIEQDS

Target ORF information:

RefSeq Version	NM_139852.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster CTCF (CTCF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139852.4

ORF Insert Sequence:

ATGCCAAGGA GGACAAAAAA GGACGAGGAC CCCGAGGATC TGCAGACCTT CCTCAACAAT 
TTCCATAAGG AGATCGAAGG CAACAGCGAT GAGAAGGTGG TAAACACGAT TTTGGAGGCC 
ATCAGCGCCG AGGCCATCGA CTTGGATGAA AATGGTGCGG AGGCTGGTGG ATCGAAACCG 
ATGGAGGAGG CGGAGGCTGA TTTGGATCAT GCGGAGGAAG CGGAGGAGGA GGAGGAGGAC 
GACGAGGATA AGTATTTCAT AGATGACGAG GGCAACTGCT ATATCAAGAC TACGCCCAAG 
AAGCAAAAGG AGCTACAAAA GAAGCTCAAA CAGGCGGCTG CCAAGCCGGG GAAAGCTACA 
CGTTCCGTGG TCAGTACAGC CACCAATAAA TCCATCAATT TGCGGCCAGC CAAATCGACA 
CCGAAAGCGA CTACTTCGAA GCCTCCCCCA GAACCCAAGG CAATTAGCGT TCGACCAGCC 
CGAGCAGCTG CAGCCAAGGC GAAGCAATCG GCGATGCCAC CGCCACCGGC ATTAGTGGTC 
AAAGTACCGG CACCCAGGGG CAGGCCTAGA AAGAACCCTG TCATACCGAA GCCTGAACCC 
ATGGACTTGG AGCGGGAGCT CGAGGAGCTG GTAGATGAGC CCGACATCAG TTCAATGGTG 
ACAGAATTGT CGGACTATAC CGTAGACGAA GCTGCCGTAG AGGCAGCAAC AGCTACGCTG 
ACGCCAAACG AGGCTGAGGT CTACGAATTT GAGGACAATG CGACGACAGA GGATGAGAAT 
GCCGACAAGA AGGACGTAGA CTTTGTGCTA AGCAACAAGG AGGTGAAGCT GAAGACAGCT 
TCTTCTACCA GCCAGAATTC GAATGCATCG GGACATAAGT ACTCATGCCC ACACTGCCCG 
TATACGGCGA GCAAGAAGTT TCTGATCACG CGCCACTCAC GAAGTCATGA CGTCGAGCCA 
TCCTTCAAGT GCTCCATCTG CGAGAGGTCC TTTCGATCGA ACGTGGGCCT GCAAAACCAC 
ATAAACACGC ACATGGGCAA TAAGCCGCAC AAGTGCAAGC TCTGCGAAAG CGCCTTCACC 
ACCAGCGGCG AGCTGGTCCG TCACACTCGC TACAAGCACA CTAAGGAAAA ACCGCACAAG 
TGCACGGAGT GCACATACGC CAGCGTGGAA CTGACCAAAC TGCGCCGCCA CATGACCTGC 
CATACGGGCG AGAGACCATA TCAATGTCCG CACTGCACCT ACGCATCGCA GGACATGTTC 
AAGCTGAAGC GACACATGGT TATCCACACC GGCGAGAAGA AGTACCAATG CGACATCTGC 
AAGTCGCGAT TTACGCAATC TAACTCACTA AAGGCCCACA AGCTCATCCA CAGCGTGGTC 
GACAAGCCCG TCTTTCAGTG CAACTACTGC CCGACGACTT GCGGTCGGAA GGCTGACCTG 
CGCGTTCACA TTAAACACAT GCACACCTCT GATGTGCCAA TGACATGCCG GCGCTGTGGC 
CAGCAGCTGC CCGATCGCTA TCAATACAAG CTGCACGTTA AGTCACACGA GGGCGAAAAG 
TGTTACAGCT GCAAGCTGTG CAGCTACGCT TCGGTCACCC AACGCCATTT GGCCTCACAC 
ATGCTCATCC ACCTGGACGA AAAGCCGTTC CATTGCGATC AGTGCCCGCA GGCCTTCCGG 
CAGCGTCAAT TGCTGCGCCG CCATATGAAT CTCGTGCACA ACGAAGAGTA TCAGCCACCA 
GAACCGCGCG AGAAACTGCA CAAGTGTCCG AGTTGTCCGC GGGAGTTTAC CCACAAGGGT 
AATCTAATGA GACACATGGA GACTCACGAT GATTCGGCGA ATGCTCGCGA AAAGCGGCGT 
CGCCTTAAAT TGGGCCGCAA TGTGCGGCTG CAGAAAGATG GCACTGTCAT CACGTTGATC 
AAGGATCAGT ATGTGGATAT GGACCGGGAC CAGGAGGAAA ACGAAGAGGA TGACAACCCA 
GAGAGCTATG ACTTGGCTGA AATCGAGCCG GAGAACTCGG AGGCTGAAGA CGCCGATGAT 
GATGTGGAAA CCATAGTCAG TGATCCGATC CGTCAGAGAA TCAAGCCAGC ACCTATCATT 
ATCAACAAAC AGGCCAGGCT TGCCGCCTCC GAAAAGCAGC CGATGATCAT CAATCAACGC 
CTCCGAAGTC AACGGGGCAC CAAGACGTTC CACATCAAGG AGGAGCCGGA CAACTCGGAC 
TTCACCGTGG AATGGCAAGG GGATGATGGC GAGGTAATGG TCGTAGAGCT GGTTAATGGC 
GACGAGGAGG TGTTGGTCAA GCACGAACCG AGCGCCAACT CCAAGATCAG TGCCAAAAAC 
TGCTTTGGTT TCGAGGACGA CGATGATTAT GAGGAGTATG GCGATGGGGA AAACGAGGTC 
GATGGCGCTT CCCAGGAGTT TCTGCAGCTG ATGGATATGA TTGAGCAGGA CTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CTCF cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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