Blimp-1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Blimp-1
Entrez Gene ID	38638
Full Name	CG5249 gene product from transcript CG5249-RF
Synonyms	BLIMP-1,CG5249,Dmel\CG5249,blimp-1,dBlimp-1,dBlimp1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 64E1-64E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001300016.1	NP_001286945.1	Blimp-1, isoform F
NM_001274514.1	NP_001261443.1	Blimp-1, isoform C
NM_001274516.1	NP_001261445.1	Blimp-1, isoform E
NM_139725.3	NP_647982.1	Blimp-1, isoform A
NM_001274515.1	NP_001261444.1	Blimp-1, isoform D
NM_001274513.1	NP_001261442.1	Blimp-1, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The timing of shade expression is determined by its transcriptional activator betaFtz-f1. The betaftz-f1 gene is activated after a decline in the expression of its transcriptional repressor Blimp-1.
Title: A biological timer in the fat body comprising Blimp-1, ?Ftz-f1 and Shade regulates pupation timing in Drosophila melanogaster.

Blimp-1 gene is transcribed during prepupal development when the ecdysteroid titer is high, but the expressed mRNA degrades rapidly.
Title: Regulatory mechanisms of ecdysone-inducible Blimp-1 encoding a transcriptional repressor that is important for the prepupal development in Drosophila.

These results suggest that the transient transcriptional repressor dBlimp-1 is important for determining developmental timing in the ecdysone-induced pathway.
Title: Drosophila Blimp-1 is a transient transcriptional repressor that controls timing of the ecdysone-induced developmental pathway.

Blimp-1 regulates terminal differentiation of the tracheal system in the Drosophila embryo.
Title: A homologue of the vertebrate SET domain and zinc finger protein Blimp-1 regulates terminal differentiation of the tracheal system in the Drosophila embryo.

The following Blimp-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Blimp-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa17613	NM_001274515.1 Latest version!	Drosophila melanogaster Blimp-1, transcript variant D (Blimp-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa17613	NM_001274516.1 Latest version!	Drosophila melanogaster Blimp-1, transcript variant E (Blimp-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa17613	NM_139725.3 Latest version!	Drosophila melanogaster Blimp-1, transcript variant A (Blimp-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa17613	NM_001300016.1 Latest version!	Drosophila melanogaster Blimp-1, transcript variant F (Blimp-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa20109	NM_001274514.1 Latest version!	Drosophila melanogaster Blimp-1, transcript variant C (Blimp-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa20109	NM_001274513.1 Latest version!	Drosophila melanogaster Blimp-1, transcript variant B (Blimp-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Blimp-1 ( NM_001300016.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286945.1 Drosophila melanogaster Blimp-1, transcript variant F (Blimp-1), mRNA.

Price & Availability↑

CloneID	OFa17613
Clone ID Related Accession (Same CDS sequence)	NM_001300016.1 , NM_001274516.1 , NM_139725.3 , NM_001274515.1
Accession Version	NM_001300016.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Blimp-1, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATGGAC ATCACAACCA CAACCATCTG CCGCAGCAGC AGCCACAAAA TCCGCAGGAG 
CAGCAGTCGG GTGCCATCAT GAAGGCAGCC AACCAGCCCC ACCATCCCGC CCACAGCCAT 
CCGCTCAATC ACCCACATCC GCACCAGCAT GCCCAGCCAC ATCCGCATCC ACATCCCCAT 
CCGCATCACA AGCTGTCGGC GAGAAGGGCG CGCAGTCGCA GTCGAAGTCG CAGTCCGACG 
CCGGATGGCA ATCAGGACTA CGATGTGACC AGGATGCGGG AGGAGGACTT CGAGCGGCTG 
GCCGTCTACC TGGTGCCGGA TGTCCAGGCG GAGCGGGGAC TGCCCAATCG GGCGGACAAG 
ACGCTGCCGC GCAGCCTGAC GCTCAAGTCA TCCATGGTCT ACTCAACGCC AAATGTTAAG 
ACTGAAGGAG TTTGGAGCAG CGGGGTCATC CCACGTGGCA CTCGCTTTGG CCCATTCGAA 
GGCATTCCAA CCTCTAATTA TCCCAATGAT AAGAATAAGG CGCGCTATTT CTGGCGGATC 
TTCAAGGACG ATGACTACTA TTACTTGGAT GGTTCCGATC GCTCGCAGTC CAATTGGATG 
CGCTATGTGG CCTCCGCTTA TAGTTTATCC GTAATGAACC TGGTGGCGTG CCAGCACCAG 
GAGAACATCT ATTTCTACAC CACCCGCGAC ATTCTGCCCA ACGAGGAGCT AATGGTTTGG 
TACTGCAAGG ACTTCGCCAG TCGCCTGGGC TACGATGTGG ATCCTGAGAC GACGATCTTC 
GGCGCATGCA AGCAGGCTGT GGAAGCGGAG GAGGAGGCGG ACGAGGAGGA GGAAGAGGCG 
GAGGGAGAGG ACGGAAAGCC GCGATACTCG ATGCCAGCTC CGGAAATTCC ACCCGATGTG 
GCCGTGAGTC ACATAACCTA CGTCATGGGT CTCCATCTGC CTGTGGGAGC GGGAAATGGT 
CCGGCGAATG GTTCGGTTGC GGGTTCCGTT TCCGGTGCCA CACCTCCACC ACCGACTGCC 
ACGCCATGCT GCCGCCGCTC CAGTCCACCC GCCCACGTCA GCACCACCAC CTCCACTTGC 
AACGCCCACC ACCCGCACAT CCAGCATGGT CGACATGCCT CCGTAATCAT CGGCCAGGAT 
CGATCGCCTA TGGCCAGCAG TGACAAGGAT ACGGCGGGAT CTCCCCTTTC CGGTCTGGAC 
CATCAGATGA CCCCGCAGGA TGGCAGCGTG AGGTCTGTGA GATCGGACGA GGGCTACCAC 
AGCAACGAGT GCCACGAGGA TGGACTCACA CCACCCGAGG ATTCCAGCGA CAGCGAGAGT 
GAACATAACT ACGTGCTGGA CTGCTCGAAG AAGGCAATTG CTCCCAAAGA GACAGTGATT 
GCCCAGGCCC AAAAGCCCAG CAGTTCTCCA CCGGCAGTGG TAATGGCCAA CACGACTACC 
AACTCGATGA TTAGCCACAG TTCACCCACT GCGACGCCCA TTTGCGAAAC GGACAAGAAT 
GAGTATAGAA AGTTCAAGGT GAAAATGCCG CTGAAGTACG AGTTCAAGAA CAAGACCTGC 
GTAAAACAGG AGCCAAGCCT TAAGGAAGTG GACCAGGAGA TGTCCTTGCC GCAGGAGGAG 
GAGGAGGATC AGGTGATGCA TCCGGAACCC GATTCAATTT GCCCATCGAC CACCACCCAT 
CTGGGCGATG AGCACATGCT GATGATGGAG CGGGAACGGG AAAGGGAGCG CGAAAGGATC 
CAGGAGAGGG AACCATCCAA CCAGCAGCCC GCCTCCTCCA CGGTCATCGT ACTGGAACAC 
AACTCTGGCG GTCAAGCCCG CACCATTGTC CCACTGAGTA AACCCTACTA CGAACCAGAT 
CCTCCCGGCG AACGCTATAT GCGATTTGGC CAGCCCTCTT CCAGCATCCT GGAGACTATC 
CTCACCAGCC AACATCGCTT GGAGGCGGCA GCTGCTGCGG CAAATGCCTG CCGACAGGCC 
AATGCCGCCA CACCACCGCC CACATCGCCC ACGGAGATGG CCTACAGCTA CAAGAAATCC 
CAGCGGTACG GTAATGCCGT GAGTCCGGAT AGTAGTTCCA ATCTGGGACA GAATCCCGAG 
CAGCTTTCCT CGTCGGCAGT GGTGGTCGGC GAACAGGAAA TGACCAGAGC CACCATGATC 
AAGGGAGAGT GCTCACCACC ACCGCCTAGC CACCATCATC ACAATGTCAT CTTCTCGCCC 
TCGAGACATG CTGCTTATCT GGGAGCCGGC GAAGCGGGTG GCCACTCACC GAGTCCGGGT 
TATCCTGGCT ATCCCCACTA TGGAGCTGCA GCCACCTCGA CCTTCCACTC ACCACCACAT 
AGTTCCCATT CCCCGTTCGA TCGCCAGTCG AATGCATCCA GTGGTGCGGG TTCGGCCACA 
AATCTGCATT TATTGCAAAC CAGCACCCAA ATGCTGAACC ATCCGCTGAT GCAGCCCCTC 
ACACCCCTGC AGCGTTTGTC CCCGCTGAGG ATATCCCCAC CCAGCAGCCT CAGTCCTGAT 
GGCAACTCGT GTCCCCGCAG CGGCAGCCCC TTGAGTCCCA ATTCCTTGGC CTCCAGAGGC 
TACCGATCGT TGCCTTATCC GCTCAAGAAG AAGGATGGCA AGATGCACTA CGAGTGCAAT 
GTGTGCTGCA AGACTTTTGG CCAATTGAGC AACCTCAAGG TGCACCTGAG AACCCATTCC 
GGCGAAAGGC CCTTCAAGTG CAATGTGTGC ACGAAGAGCT TCACCCAGTT GGCGCACCTC 
CAGAAGCATC ATCTGGTGCA TACGGGCGAG AAACCGCATC AGTGCGACAT CTGCAAGAAG 
CGTTTCTCGA GCACCTCCAA CCTGAAGACC CATTTGAGGC TGCACAGTGG TCAGAAACCA 
TATGCCTGTG ATCTCTGCCC GCAGAAGTTC ACACAGTTTG TGCATCTGAA GTTGCACAAG 
AGACTGCACA CGAACGACAG GCCCTATGTG TGCCAGGGTT GCGACAAGAA GTACATCAGT 
GCATCCGGCT TGAGGACCCA CTGGAAGACC ACCAGCTGCA AGCCCAACAA TCTGGAGGAG 
GAGCTGGCCA TGGCAGCAGC GGCAACATCG GAGTGTTTGG ATAAAGACCA TCCCGAACCG 
GACTCCCGAG AAGCCTACGA GCAACTGACG CAGCACATGC ATCCTGCTGT GCATCCGGGA 
TTGAGGCACC TCTCGAGTGG GGGTCAATCG CCACCGCGCC TGATACCCCT GGGCAATCAC 
ATGGCGCCAC AACAGCAGTC GCATCAGCAG CAGCAACAGC AGCATCAGCA GCAGGGAGTG 
CCACCGCCCC ACTTACTGAT GACCCAGCAT TCGGTGGGTC CGGCACCCAT GCTGCTGACC 
ACCGCCAGCC AGTTGCCACC ACCGCCGCCA CATCATCAGC AGAACTCGCC CAGCCGTCTG 
CTGCAACATG GCCACGCACA TCCGTTGCAG ATGCAACAGC AACAGCAGCA GCAACAGCAG 
CACTCGCCCA AGGGACTCAA ATCCCTGCCG GAATCGGGGG TGTATCTGCA TGGACAGCAT 
GTGCAGGCCC AGGAGAGCAG ACCATCGGTC ATTGAGTCCT CCAACCAGCC CATGATCATC 
GAATGCACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286945.1
CDS	626..4237
Translation	MHGHHNHNHLPQQQPQNPQEQQSGAIMKAANQPHHPAHSHPLNHPHPHQHAQPHPHPHPHPHHKLSARRARSRSRSRSPTPDGNQDYDVTRMREEDFERLAVYLVPDVQAERGLPNRADKTLPRSLTLKSSMVYSTPNVKTEGVWSSGVIPRGTRFGPFEGIPTSNYPNDKNKARYFWRIFKDDDYYYLDGSDRSQSNWMRYVASAYSLSVMNLVACQHQENIYFYTTRDILPNEELMVWYCKDFASRLGYDVDPETTIFGACKQAVEAEEEADEEEEEAEGEDGKPRYSMPAPEIPPDVAVSHITYVMGLHLPVGAGNGPANGSVAGSVSGATPPPPTATPCCRRSSPPAHVSTTTSTCNAHHPHIQHGRHASVIIGQDRSPMASSDKDTAGSPLSGLDHQMTPQDGSVRSVRSDEGYHSNECHEDGLTPPEDSSDSESEHNYVLDCSKKAIAPKETVIAQAQKPSSSPPAVVMANTTTNSMISHSSPTATPICETDKNEYRKFKVKMPLKYEFKNKTCVKQEPSLKEVDQEMSLPQEEEEDQVMHPEPDSICPSTTTHLGDEHMLMMERERERERERIQEREPSNQQPASSTVIVLEHNSGGQARTIVPLSKPYYEPDPPGERYMRFGQPSSSILETILTSQHRLEAAAAAANACRQANAATPPPTSPTEMAYSYKKSQRYGNAVSPDSSSNLGQNPEQLSSSAVVVGEQEMTRATMIKGECSPPPPSHHHHNVIFSPSRHAAYLGAGEAGGHSPSPGYPGYPHYGAAATSTFHSPPHSSHSPFDRQSNASSGAGSATNLHLLQTSTQMLNHPLMQPLTPLQRLSPLRISPPSSLSPDGNSCPRSGSPLSPNSLASRGYRSLPYPLKKKDGKMHYECNVCCKTFGQLSNLKVHLRTHSGERPFKCNVCTKSFTQLAHLQKHHLVHTGEKPHQCDICKKRFSSTSNLKTHLRLHSGQKPYACDLCPQKFTQFVHLKLHKRLHTNDRPYVCQGCDKKYISASGLRTHWKTTSCKPNNLEEELAMAAAATSECLDKDHPEPDSREAYEQLTQHMHPAVHPGLRHLSSGGQSPPRLIPLGNHMAPQQQSHQQQQQQHQQQGVPPPHLLMTQHSVGPAPMLLTTASQLPPPPPHHQQNSPSRLLQHGHAHPLQMQQQQQQQQQHSPKGLKSLPESGVYLHGQHVQAQESRPSVIESSNQPMIIECT

Target ORF information:

RefSeq Version	NM_001300016.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Blimp-1, transcript variant F (Blimp-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300016.1

ORF Insert Sequence:

ATGCATGGAC ATCACAACCA CAACCATCTG CCGCAGCAGC AGCCACAAAA TCCGCAGGAG 
CAGCAGTCGG GTGCCATCAT GAAGGCAGCC AACCAGCCCC ACCATCCCGC CCACAGCCAT 
CCGCTCAATC ACCCACATCC GCACCAGCAT GCCCAGCCAC ATCCGCATCC ACATCCCCAT 
CCGCATCACA AGCTGTCGGC GAGAAGGGCG CGCAGTCGCA GTCGAAGTCG CAGTCCGACG 
CCGGATGGCA ATCAGGACTA CGATGTGACC AGGATGCGGG AGGAGGACTT CGAGCGGCTG 
GCCGTCTACC TGGTGCCGGA TGTCCAGGCG GAGCGGGGAC TGCCCAATCG GGCGGACAAG 
ACGCTGCCGC GCAGCCTGAC GCTCAAGTCA TCCATGGTCT ACTCAACGCC AAATGTTAAG 
ACTGAAGGAG TTTGGAGCAG CGGGGTCATC CCACGTGGCA CTCGCTTTGG CCCATTCGAA 
GGCATTCCAA CCTCTAATTA TCCCAATGAT AAGAATAAGG CGCGCTATTT CTGGCGGATC 
TTCAAGGACG ATGACTACTA TTACTTGGAT GGTTCCGATC GCTCGCAGTC CAATTGGATG 
CGCTATGTGG CCTCCGCTTA TAGTTTATCC GTAATGAACC TGGTGGCGTG CCAGCACCAG 
GAGAACATCT ATTTCTACAC CACCCGCGAC ATTCTGCCCA ACGAGGAGCT AATGGTTTGG 
TACTGCAAGG ACTTCGCCAG TCGCCTGGGC TACGATGTGG ATCCTGAGAC GACGATCTTC 
GGCGCATGCA AGCAGGCTGT GGAAGCGGAG GAGGAGGCGG ACGAGGAGGA GGAAGAGGCG 
GAGGGAGAGG ACGGAAAGCC GCGATACTCG ATGCCAGCTC CGGAAATTCC ACCCGATGTG 
GCCGTGAGTC ACATAACCTA CGTCATGGGT CTCCATCTGC CTGTGGGAGC GGGAAATGGT 
CCGGCGAATG GTTCGGTTGC GGGTTCCGTT TCCGGTGCCA CACCTCCACC ACCGACTGCC 
ACGCCATGCT GCCGCCGCTC CAGTCCACCC GCCCACGTCA GCACCACCAC CTCCACTTGC 
AACGCCCACC ACCCGCACAT CCAGCATGGT CGACATGCCT CCGTAATCAT CGGCCAGGAT 
CGATCGCCTA TGGCCAGCAG TGACAAGGAT ACGGCGGGAT CTCCCCTTTC CGGTCTGGAC 
CATCAGATGA CCCCGCAGGA TGGCAGCGTG AGGTCTGTGA GATCGGACGA GGGCTACCAC 
AGCAACGAGT GCCACGAGGA TGGACTCACA CCACCCGAGG ATTCCAGCGA CAGCGAGAGT 
GAACATAACT ACGTGCTGGA CTGCTCGAAG AAGGCAATTG CTCCCAAAGA GACAGTGATT 
GCCCAGGCCC AAAAGCCCAG CAGTTCTCCA CCGGCAGTGG TAATGGCCAA CACGACTACC 
AACTCGATGA TTAGCCACAG TTCACCCACT GCGACGCCCA TTTGCGAAAC GGACAAGAAT 
GAGTATAGAA AGTTCAAGGT GAAAATGCCG CTGAAGTACG AGTTCAAGAA CAAGACCTGC 
GTAAAACAGG AGCCAAGCCT TAAGGAAGTG GACCAGGAGA TGTCCTTGCC GCAGGAGGAG 
GAGGAGGATC AGGTGATGCA TCCGGAACCC GATTCAATTT GCCCATCGAC CACCACCCAT 
CTGGGCGATG AGCACATGCT GATGATGGAG CGGGAACGGG AAAGGGAGCG CGAAAGGATC 
CAGGAGAGGG AACCATCCAA CCAGCAGCCC GCCTCCTCCA CGGTCATCGT ACTGGAACAC 
AACTCTGGCG GTCAAGCCCG CACCATTGTC CCACTGAGTA AACCCTACTA CGAACCAGAT 
CCTCCCGGCG AACGCTATAT GCGATTTGGC CAGCCCTCTT CCAGCATCCT GGAGACTATC 
CTCACCAGCC AACATCGCTT GGAGGCGGCA GCTGCTGCGG CAAATGCCTG CCGACAGGCC 
AATGCCGCCA CACCACCGCC CACATCGCCC ACGGAGATGG CCTACAGCTA CAAGAAATCC 
CAGCGGTACG GTAATGCCGT GAGTCCGGAT AGTAGTTCCA ATCTGGGACA GAATCCCGAG 
CAGCTTTCCT CGTCGGCAGT GGTGGTCGGC GAACAGGAAA TGACCAGAGC CACCATGATC 
AAGGGAGAGT GCTCACCACC ACCGCCTAGC CACCATCATC ACAATGTCAT CTTCTCGCCC 
TCGAGACATG CTGCTTATCT GGGAGCCGGC GAAGCGGGTG GCCACTCACC GAGTCCGGGT 
TATCCTGGCT ATCCCCACTA TGGAGCTGCA GCCACCTCGA CCTTCCACTC ACCACCACAT 
AGTTCCCATT CCCCGTTCGA TCGCCAGTCG AATGCATCCA GTGGTGCGGG TTCGGCCACA 
AATCTGCATT TATTGCAAAC CAGCACCCAA ATGCTGAACC ATCCGCTGAT GCAGCCCCTC 
ACACCCCTGC AGCGTTTGTC CCCGCTGAGG ATATCCCCAC CCAGCAGCCT CAGTCCTGAT 
GGCAACTCGT GTCCCCGCAG CGGCAGCCCC TTGAGTCCCA ATTCCTTGGC CTCCAGAGGC 
TACCGATCGT TGCCTTATCC GCTCAAGAAG AAGGATGGCA AGATGCACTA CGAGTGCAAT 
GTGTGCTGCA AGACTTTTGG CCAATTGAGC AACCTCAAGG TGCACCTGAG AACCCATTCC 
GGCGAAAGGC CCTTCAAGTG CAATGTGTGC ACGAAGAGCT TCACCCAGTT GGCGCACCTC 
CAGAAGCATC ATCTGGTGCA TACGGGCGAG AAACCGCATC AGTGCGACAT CTGCAAGAAG 
CGTTTCTCGA GCACCTCCAA CCTGAAGACC CATTTGAGGC TGCACAGTGG TCAGAAACCA 
TATGCCTGTG ATCTCTGCCC GCAGAAGTTC ACACAGTTTG TGCATCTGAA GTTGCACAAG 
AGACTGCACA CGAACGACAG GCCCTATGTG TGCCAGGGTT GCGACAAGAA GTACATCAGT 
GCATCCGGCT TGAGGACCCA CTGGAAGACC ACCAGCTGCA AGCCCAACAA TCTGGAGGAG 
GAGCTGGCCA TGGCAGCAGC GGCAACATCG GAGTGTTTGG ATAAAGACCA TCCCGAACCG 
GACTCCCGAG AAGCCTACGA GCAACTGACG CAGCACATGC ATCCTGCTGT GCATCCGGGA 
TTGAGGCACC TCTCGAGTGG GGGTCAATCG CCACCGCGCC TGATACCCCT GGGCAATCAC 
ATGGCGCCAC AACAGCAGTC GCATCAGCAG CAGCAACAGC AGCATCAGCA GCAGGGAGTG 
CCACCGCCCC ACTTACTGAT GACCCAGCAT TCGGTGGGTC CGGCACCCAT GCTGCTGACC 
ACCGCCAGCC AGTTGCCACC ACCGCCGCCA CATCATCAGC AGAACTCGCC CAGCCGTCTG 
CTGCAACATG GCCACGCACA TCCGTTGCAG ATGCAACAGC AACAGCAGCA GCAACAGCAG 
CACTCGCCCA AGGGACTCAA ATCCCTGCCG GAATCGGGGG TGTATCTGCA TGGACAGCAT 
GTGCAGGCCC AGGAGAGCAG ACCATCGGTC ATTGAGTCCT CCAACCAGCC CATGATCATC 
GAATGCACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Blimp-1 ( NM_001274516.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261445.1 Drosophila melanogaster Blimp-1, transcript variant E (Blimp-1), mRNA.

Price & Availability↑

CloneID	OFa17613
Clone ID Related Accession (Same CDS sequence)	NM_001300016.1 , NM_001274516.1 , NM_139725.3 , NM_001274515.1
Accession Version	NM_001274516.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Blimp-1, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATGGAC ATCACAACCA CAACCATCTG CCGCAGCAGC AGCCACAAAA TCCGCAGGAG 
CAGCAGTCGG GTGCCATCAT GAAGGCAGCC AACCAGCCCC ACCATCCCGC CCACAGCCAT 
CCGCTCAATC ACCCACATCC GCACCAGCAT GCCCAGCCAC ATCCGCATCC ACATCCCCAT 
CCGCATCACA AGCTGTCGGC GAGAAGGGCG CGCAGTCGCA GTCGAAGTCG CAGTCCGACG 
CCGGATGGCA ATCAGGACTA CGATGTGACC AGGATGCGGG AGGAGGACTT CGAGCGGCTG 
GCCGTCTACC TGGTGCCGGA TGTCCAGGCG GAGCGGGGAC TGCCCAATCG GGCGGACAAG 
ACGCTGCCGC GCAGCCTGAC GCTCAAGTCA TCCATGGTCT ACTCAACGCC AAATGTTAAG 
ACTGAAGGAG TTTGGAGCAG CGGGGTCATC CCACGTGGCA CTCGCTTTGG CCCATTCGAA 
GGCATTCCAA CCTCTAATTA TCCCAATGAT AAGAATAAGG CGCGCTATTT CTGGCGGATC 
TTCAAGGACG ATGACTACTA TTACTTGGAT GGTTCCGATC GCTCGCAGTC CAATTGGATG 
CGCTATGTGG CCTCCGCTTA TAGTTTATCC GTAATGAACC TGGTGGCGTG CCAGCACCAG 
GAGAACATCT ATTTCTACAC CACCCGCGAC ATTCTGCCCA ACGAGGAGCT AATGGTTTGG 
TACTGCAAGG ACTTCGCCAG TCGCCTGGGC TACGATGTGG ATCCTGAGAC GACGATCTTC 
GGCGCATGCA AGCAGGCTGT GGAAGCGGAG GAGGAGGCGG ACGAGGAGGA GGAAGAGGCG 
GAGGGAGAGG ACGGAAAGCC GCGATACTCG ATGCCAGCTC CGGAAATTCC ACCCGATGTG 
GCCGTGAGTC ACATAACCTA CGTCATGGGT CTCCATCTGC CTGTGGGAGC GGGAAATGGT 
CCGGCGAATG GTTCGGTTGC GGGTTCCGTT TCCGGTGCCA CACCTCCACC ACCGACTGCC 
ACGCCATGCT GCCGCCGCTC CAGTCCACCC GCCCACGTCA GCACCACCAC CTCCACTTGC 
AACGCCCACC ACCCGCACAT CCAGCATGGT CGACATGCCT CCGTAATCAT CGGCCAGGAT 
CGATCGCCTA TGGCCAGCAG TGACAAGGAT ACGGCGGGAT CTCCCCTTTC CGGTCTGGAC 
CATCAGATGA CCCCGCAGGA TGGCAGCGTG AGGTCTGTGA GATCGGACGA GGGCTACCAC 
AGCAACGAGT GCCACGAGGA TGGACTCACA CCACCCGAGG ATTCCAGCGA CAGCGAGAGT 
GAACATAACT ACGTGCTGGA CTGCTCGAAG AAGGCAATTG CTCCCAAAGA GACAGTGATT 
GCCCAGGCCC AAAAGCCCAG CAGTTCTCCA CCGGCAGTGG TAATGGCCAA CACGACTACC 
AACTCGATGA TTAGCCACAG TTCACCCACT GCGACGCCCA TTTGCGAAAC GGACAAGAAT 
GAGTATAGAA AGTTCAAGGT GAAAATGCCG CTGAAGTACG AGTTCAAGAA CAAGACCTGC 
GTAAAACAGG AGCCAAGCCT TAAGGAAGTG GACCAGGAGA TGTCCTTGCC GCAGGAGGAG 
GAGGAGGATC AGGTGATGCA TCCGGAACCC GATTCAATTT GCCCATCGAC CACCACCCAT 
CTGGGCGATG AGCACATGCT GATGATGGAG CGGGAACGGG AAAGGGAGCG CGAAAGGATC 
CAGGAGAGGG AACCATCCAA CCAGCAGCCC GCCTCCTCCA CGGTCATCGT ACTGGAACAC 
AACTCTGGCG GTCAAGCCCG CACCATTGTC CCACTGAGTA AACCCTACTA CGAACCAGAT 
CCTCCCGGCG AACGCTATAT GCGATTTGGC CAGCCCTCTT CCAGCATCCT GGAGACTATC 
CTCACCAGCC AACATCGCTT GGAGGCGGCA GCTGCTGCGG CAAATGCCTG CCGACAGGCC 
AATGCCGCCA CACCACCGCC CACATCGCCC ACGGAGATGG CCTACAGCTA CAAGAAATCC 
CAGCGGTACG GTAATGCCGT GAGTCCGGAT AGTAGTTCCA ATCTGGGACA GAATCCCGAG 
CAGCTTTCCT CGTCGGCAGT GGTGGTCGGC GAACAGGAAA TGACCAGAGC CACCATGATC 
AAGGGAGAGT GCTCACCACC ACCGCCTAGC CACCATCATC ACAATGTCAT CTTCTCGCCC 
TCGAGACATG CTGCTTATCT GGGAGCCGGC GAAGCGGGTG GCCACTCACC GAGTCCGGGT 
TATCCTGGCT ATCCCCACTA TGGAGCTGCA GCCACCTCGA CCTTCCACTC ACCACCACAT 
AGTTCCCATT CCCCGTTCGA TCGCCAGTCG AATGCATCCA GTGGTGCGGG TTCGGCCACA 
AATCTGCATT TATTGCAAAC CAGCACCCAA ATGCTGAACC ATCCGCTGAT GCAGCCCCTC 
ACACCCCTGC AGCGTTTGTC CCCGCTGAGG ATATCCCCAC CCAGCAGCCT CAGTCCTGAT 
GGCAACTCGT GTCCCCGCAG CGGCAGCCCC TTGAGTCCCA ATTCCTTGGC CTCCAGAGGC 
TACCGATCGT TGCCTTATCC GCTCAAGAAG AAGGATGGCA AGATGCACTA CGAGTGCAAT 
GTGTGCTGCA AGACTTTTGG CCAATTGAGC AACCTCAAGG TGCACCTGAG AACCCATTCC 
GGCGAAAGGC CCTTCAAGTG CAATGTGTGC ACGAAGAGCT TCACCCAGTT GGCGCACCTC 
CAGAAGCATC ATCTGGTGCA TACGGGCGAG AAACCGCATC AGTGCGACAT CTGCAAGAAG 
CGTTTCTCGA GCACCTCCAA CCTGAAGACC CATTTGAGGC TGCACAGTGG TCAGAAACCA 
TATGCCTGTG ATCTCTGCCC GCAGAAGTTC ACACAGTTTG TGCATCTGAA GTTGCACAAG 
AGACTGCACA CGAACGACAG GCCCTATGTG TGCCAGGGTT GCGACAAGAA GTACATCAGT 
GCATCCGGCT TGAGGACCCA CTGGAAGACC ACCAGCTGCA AGCCCAACAA TCTGGAGGAG 
GAGCTGGCCA TGGCAGCAGC GGCAACATCG GAGTGTTTGG ATAAAGACCA TCCCGAACCG 
GACTCCCGAG AAGCCTACGA GCAACTGACG CAGCACATGC ATCCTGCTGT GCATCCGGGA 
TTGAGGCACC TCTCGAGTGG GGGTCAATCG CCACCGCGCC TGATACCCCT GGGCAATCAC 
ATGGCGCCAC AACAGCAGTC GCATCAGCAG CAGCAACAGC AGCATCAGCA GCAGGGAGTG 
CCACCGCCCC ACTTACTGAT GACCCAGCAT TCGGTGGGTC CGGCACCCAT GCTGCTGACC 
ACCGCCAGCC AGTTGCCACC ACCGCCGCCA CATCATCAGC AGAACTCGCC CAGCCGTCTG 
CTGCAACATG GCCACGCACA TCCGTTGCAG ATGCAACAGC AACAGCAGCA GCAACAGCAG 
CACTCGCCCA AGGGACTCAA ATCCCTGCCG GAATCGGGGG TGTATCTGCA TGGACAGCAT 
GTGCAGGCCC AGGAGAGCAG ACCATCGGTC ATTGAGTCCT CCAACCAGCC CATGATCATC 
GAATGCACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261445.1
CDS	196..3807
Translation	MHGHHNHNHLPQQQPQNPQEQQSGAIMKAANQPHHPAHSHPLNHPHPHQHAQPHPHPHPHPHHKLSARRARSRSRSRSPTPDGNQDYDVTRMREEDFERLAVYLVPDVQAERGLPNRADKTLPRSLTLKSSMVYSTPNVKTEGVWSSGVIPRGTRFGPFEGIPTSNYPNDKNKARYFWRIFKDDDYYYLDGSDRSQSNWMRYVASAYSLSVMNLVACQHQENIYFYTTRDILPNEELMVWYCKDFASRLGYDVDPETTIFGACKQAVEAEEEADEEEEEAEGEDGKPRYSMPAPEIPPDVAVSHITYVMGLHLPVGAGNGPANGSVAGSVSGATPPPPTATPCCRRSSPPAHVSTTTSTCNAHHPHIQHGRHASVIIGQDRSPMASSDKDTAGSPLSGLDHQMTPQDGSVRSVRSDEGYHSNECHEDGLTPPEDSSDSESEHNYVLDCSKKAIAPKETVIAQAQKPSSSPPAVVMANTTTNSMISHSSPTATPICETDKNEYRKFKVKMPLKYEFKNKTCVKQEPSLKEVDQEMSLPQEEEEDQVMHPEPDSICPSTTTHLGDEHMLMMERERERERERIQEREPSNQQPASSTVIVLEHNSGGQARTIVPLSKPYYEPDPPGERYMRFGQPSSSILETILTSQHRLEAAAAAANACRQANAATPPPTSPTEMAYSYKKSQRYGNAVSPDSSSNLGQNPEQLSSSAVVVGEQEMTRATMIKGECSPPPPSHHHHNVIFSPSRHAAYLGAGEAGGHSPSPGYPGYPHYGAAATSTFHSPPHSSHSPFDRQSNASSGAGSATNLHLLQTSTQMLNHPLMQPLTPLQRLSPLRISPPSSLSPDGNSCPRSGSPLSPNSLASRGYRSLPYPLKKKDGKMHYECNVCCKTFGQLSNLKVHLRTHSGERPFKCNVCTKSFTQLAHLQKHHLVHTGEKPHQCDICKKRFSSTSNLKTHLRLHSGQKPYACDLCPQKFTQFVHLKLHKRLHTNDRPYVCQGCDKKYISASGLRTHWKTTSCKPNNLEEELAMAAAATSECLDKDHPEPDSREAYEQLTQHMHPAVHPGLRHLSSGGQSPPRLIPLGNHMAPQQQSHQQQQQQHQQQGVPPPHLLMTQHSVGPAPMLLTTASQLPPPPPHHQQNSPSRLLQHGHAHPLQMQQQQQQQQQHSPKGLKSLPESGVYLHGQHVQAQESRPSVIESSNQPMIIECT

Target ORF information:

RefSeq Version	NM_001274516.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Blimp-1, transcript variant E (Blimp-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274516.1

ORF Insert Sequence:

ATGCATGGAC ATCACAACCA CAACCATCTG CCGCAGCAGC AGCCACAAAA TCCGCAGGAG 
CAGCAGTCGG GTGCCATCAT GAAGGCAGCC AACCAGCCCC ACCATCCCGC CCACAGCCAT 
CCGCTCAATC ACCCACATCC GCACCAGCAT GCCCAGCCAC ATCCGCATCC ACATCCCCAT 
CCGCATCACA AGCTGTCGGC GAGAAGGGCG CGCAGTCGCA GTCGAAGTCG CAGTCCGACG 
CCGGATGGCA ATCAGGACTA CGATGTGACC AGGATGCGGG AGGAGGACTT CGAGCGGCTG 
GCCGTCTACC TGGTGCCGGA TGTCCAGGCG GAGCGGGGAC TGCCCAATCG GGCGGACAAG 
ACGCTGCCGC GCAGCCTGAC GCTCAAGTCA TCCATGGTCT ACTCAACGCC AAATGTTAAG 
ACTGAAGGAG TTTGGAGCAG CGGGGTCATC CCACGTGGCA CTCGCTTTGG CCCATTCGAA 
GGCATTCCAA CCTCTAATTA TCCCAATGAT AAGAATAAGG CGCGCTATTT CTGGCGGATC 
TTCAAGGACG ATGACTACTA TTACTTGGAT GGTTCCGATC GCTCGCAGTC CAATTGGATG 
CGCTATGTGG CCTCCGCTTA TAGTTTATCC GTAATGAACC TGGTGGCGTG CCAGCACCAG 
GAGAACATCT ATTTCTACAC CACCCGCGAC ATTCTGCCCA ACGAGGAGCT AATGGTTTGG 
TACTGCAAGG ACTTCGCCAG TCGCCTGGGC TACGATGTGG ATCCTGAGAC GACGATCTTC 
GGCGCATGCA AGCAGGCTGT GGAAGCGGAG GAGGAGGCGG ACGAGGAGGA GGAAGAGGCG 
GAGGGAGAGG ACGGAAAGCC GCGATACTCG ATGCCAGCTC CGGAAATTCC ACCCGATGTG 
GCCGTGAGTC ACATAACCTA CGTCATGGGT CTCCATCTGC CTGTGGGAGC GGGAAATGGT 
CCGGCGAATG GTTCGGTTGC GGGTTCCGTT TCCGGTGCCA CACCTCCACC ACCGACTGCC 
ACGCCATGCT GCCGCCGCTC CAGTCCACCC GCCCACGTCA GCACCACCAC CTCCACTTGC 
AACGCCCACC ACCCGCACAT CCAGCATGGT CGACATGCCT CCGTAATCAT CGGCCAGGAT 
CGATCGCCTA TGGCCAGCAG TGACAAGGAT ACGGCGGGAT CTCCCCTTTC CGGTCTGGAC 
CATCAGATGA CCCCGCAGGA TGGCAGCGTG AGGTCTGTGA GATCGGACGA GGGCTACCAC 
AGCAACGAGT GCCACGAGGA TGGACTCACA CCACCCGAGG ATTCCAGCGA CAGCGAGAGT 
GAACATAACT ACGTGCTGGA CTGCTCGAAG AAGGCAATTG CTCCCAAAGA GACAGTGATT 
GCCCAGGCCC AAAAGCCCAG CAGTTCTCCA CCGGCAGTGG TAATGGCCAA CACGACTACC 
AACTCGATGA TTAGCCACAG TTCACCCACT GCGACGCCCA TTTGCGAAAC GGACAAGAAT 
GAGTATAGAA AGTTCAAGGT GAAAATGCCG CTGAAGTACG AGTTCAAGAA CAAGACCTGC 
GTAAAACAGG AGCCAAGCCT TAAGGAAGTG GACCAGGAGA TGTCCTTGCC GCAGGAGGAG 
GAGGAGGATC AGGTGATGCA TCCGGAACCC GATTCAATTT GCCCATCGAC CACCACCCAT 
CTGGGCGATG AGCACATGCT GATGATGGAG CGGGAACGGG AAAGGGAGCG CGAAAGGATC 
CAGGAGAGGG AACCATCCAA CCAGCAGCCC GCCTCCTCCA CGGTCATCGT ACTGGAACAC 
AACTCTGGCG GTCAAGCCCG CACCATTGTC CCACTGAGTA AACCCTACTA CGAACCAGAT 
CCTCCCGGCG AACGCTATAT GCGATTTGGC CAGCCCTCTT CCAGCATCCT GGAGACTATC 
CTCACCAGCC AACATCGCTT GGAGGCGGCA GCTGCTGCGG CAAATGCCTG CCGACAGGCC 
AATGCCGCCA CACCACCGCC CACATCGCCC ACGGAGATGG CCTACAGCTA CAAGAAATCC 
CAGCGGTACG GTAATGCCGT GAGTCCGGAT AGTAGTTCCA ATCTGGGACA GAATCCCGAG 
CAGCTTTCCT CGTCGGCAGT GGTGGTCGGC GAACAGGAAA TGACCAGAGC CACCATGATC 
AAGGGAGAGT GCTCACCACC ACCGCCTAGC CACCATCATC ACAATGTCAT CTTCTCGCCC 
TCGAGACATG CTGCTTATCT GGGAGCCGGC GAAGCGGGTG GCCACTCACC GAGTCCGGGT 
TATCCTGGCT ATCCCCACTA TGGAGCTGCA GCCACCTCGA CCTTCCACTC ACCACCACAT 
AGTTCCCATT CCCCGTTCGA TCGCCAGTCG AATGCATCCA GTGGTGCGGG TTCGGCCACA 
AATCTGCATT TATTGCAAAC CAGCACCCAA ATGCTGAACC ATCCGCTGAT GCAGCCCCTC 
ACACCCCTGC AGCGTTTGTC CCCGCTGAGG ATATCCCCAC CCAGCAGCCT CAGTCCTGAT 
GGCAACTCGT GTCCCCGCAG CGGCAGCCCC TTGAGTCCCA ATTCCTTGGC CTCCAGAGGC 
TACCGATCGT TGCCTTATCC GCTCAAGAAG AAGGATGGCA AGATGCACTA CGAGTGCAAT 
GTGTGCTGCA AGACTTTTGG CCAATTGAGC AACCTCAAGG TGCACCTGAG AACCCATTCC 
GGCGAAAGGC CCTTCAAGTG CAATGTGTGC ACGAAGAGCT TCACCCAGTT GGCGCACCTC 
CAGAAGCATC ATCTGGTGCA TACGGGCGAG AAACCGCATC AGTGCGACAT CTGCAAGAAG 
CGTTTCTCGA GCACCTCCAA CCTGAAGACC CATTTGAGGC TGCACAGTGG TCAGAAACCA 
TATGCCTGTG ATCTCTGCCC GCAGAAGTTC ACACAGTTTG TGCATCTGAA GTTGCACAAG 
AGACTGCACA CGAACGACAG GCCCTATGTG TGCCAGGGTT GCGACAAGAA GTACATCAGT 
GCATCCGGCT TGAGGACCCA CTGGAAGACC ACCAGCTGCA AGCCCAACAA TCTGGAGGAG 
GAGCTGGCCA TGGCAGCAGC GGCAACATCG GAGTGTTTGG ATAAAGACCA TCCCGAACCG 
GACTCCCGAG AAGCCTACGA GCAACTGACG CAGCACATGC ATCCTGCTGT GCATCCGGGA 
TTGAGGCACC TCTCGAGTGG GGGTCAATCG CCACCGCGCC TGATACCCCT GGGCAATCAC 
ATGGCGCCAC AACAGCAGTC GCATCAGCAG CAGCAACAGC AGCATCAGCA GCAGGGAGTG 
CCACCGCCCC ACTTACTGAT GACCCAGCAT TCGGTGGGTC CGGCACCCAT GCTGCTGACC 
ACCGCCAGCC AGTTGCCACC ACCGCCGCCA CATCATCAGC AGAACTCGCC CAGCCGTCTG 
CTGCAACATG GCCACGCACA TCCGTTGCAG ATGCAACAGC AACAGCAGCA GCAACAGCAG 
CACTCGCCCA AGGGACTCAA ATCCCTGCCG GAATCGGGGG TGTATCTGCA TGGACAGCAT 
GTGCAGGCCC AGGAGAGCAG ACCATCGGTC ATTGAGTCCT CCAACCAGCC CATGATCATC 
GAATGCACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Blimp-1 ( NM_139725.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_647982.1 Drosophila melanogaster Blimp-1, transcript variant A (Blimp-1), mRNA.

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CloneID	OFa17613
Clone ID Related Accession (Same CDS sequence)	NM_001300016.1 , NM_001274516.1 , NM_139725.3 , NM_001274515.1
Accession Version	NM_139725.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Blimp-1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_139725.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATGGAC ATCACAACCA CAACCATCTG CCGCAGCAGC AGCCACAAAA TCCGCAGGAG 
CAGCAGTCGG GTGCCATCAT GAAGGCAGCC AACCAGCCCC ACCATCCCGC CCACAGCCAT 
CCGCTCAATC ACCCACATCC GCACCAGCAT GCCCAGCCAC ATCCGCATCC ACATCCCCAT 
CCGCATCACA AGCTGTCGGC GAGAAGGGCG CGCAGTCGCA GTCGAAGTCG CAGTCCGACG 
CCGGATGGCA ATCAGGACTA CGATGTGACC AGGATGCGGG AGGAGGACTT CGAGCGGCTG 
GCCGTCTACC TGGTGCCGGA TGTCCAGGCG GAGCGGGGAC TGCCCAATCG GGCGGACAAG 
ACGCTGCCGC GCAGCCTGAC GCTCAAGTCA TCCATGGTCT ACTCAACGCC AAATGTTAAG 
ACTGAAGGAG TTTGGAGCAG CGGGGTCATC CCACGTGGCA CTCGCTTTGG CCCATTCGAA 
GGCATTCCAA CCTCTAATTA TCCCAATGAT AAGAATAAGG CGCGCTATTT CTGGCGGATC 
TTCAAGGACG ATGACTACTA TTACTTGGAT GGTTCCGATC GCTCGCAGTC CAATTGGATG 
CGCTATGTGG CCTCCGCTTA TAGTTTATCC GTAATGAACC TGGTGGCGTG CCAGCACCAG 
GAGAACATCT ATTTCTACAC CACCCGCGAC ATTCTGCCCA ACGAGGAGCT AATGGTTTGG 
TACTGCAAGG ACTTCGCCAG TCGCCTGGGC TACGATGTGG ATCCTGAGAC GACGATCTTC 
GGCGCATGCA AGCAGGCTGT GGAAGCGGAG GAGGAGGCGG ACGAGGAGGA GGAAGAGGCG 
GAGGGAGAGG ACGGAAAGCC GCGATACTCG ATGCCAGCTC CGGAAATTCC ACCCGATGTG 
GCCGTGAGTC ACATAACCTA CGTCATGGGT CTCCATCTGC CTGTGGGAGC GGGAAATGGT 
CCGGCGAATG GTTCGGTTGC GGGTTCCGTT TCCGGTGCCA CACCTCCACC ACCGACTGCC 
ACGCCATGCT GCCGCCGCTC CAGTCCACCC GCCCACGTCA GCACCACCAC CTCCACTTGC 
AACGCCCACC ACCCGCACAT CCAGCATGGT CGACATGCCT CCGTAATCAT CGGCCAGGAT 
CGATCGCCTA TGGCCAGCAG TGACAAGGAT ACGGCGGGAT CTCCCCTTTC CGGTCTGGAC 
CATCAGATGA CCCCGCAGGA TGGCAGCGTG AGGTCTGTGA GATCGGACGA GGGCTACCAC 
AGCAACGAGT GCCACGAGGA TGGACTCACA CCACCCGAGG ATTCCAGCGA CAGCGAGAGT 
GAACATAACT ACGTGCTGGA CTGCTCGAAG AAGGCAATTG CTCCCAAAGA GACAGTGATT 
GCCCAGGCCC AAAAGCCCAG CAGTTCTCCA CCGGCAGTGG TAATGGCCAA CACGACTACC 
AACTCGATGA TTAGCCACAG TTCACCCACT GCGACGCCCA TTTGCGAAAC GGACAAGAAT 
GAGTATAGAA AGTTCAAGGT GAAAATGCCG CTGAAGTACG AGTTCAAGAA CAAGACCTGC 
GTAAAACAGG AGCCAAGCCT TAAGGAAGTG GACCAGGAGA TGTCCTTGCC GCAGGAGGAG 
GAGGAGGATC AGGTGATGCA TCCGGAACCC GATTCAATTT GCCCATCGAC CACCACCCAT 
CTGGGCGATG AGCACATGCT GATGATGGAG CGGGAACGGG AAAGGGAGCG CGAAAGGATC 
CAGGAGAGGG AACCATCCAA CCAGCAGCCC GCCTCCTCCA CGGTCATCGT ACTGGAACAC 
AACTCTGGCG GTCAAGCCCG CACCATTGTC CCACTGAGTA AACCCTACTA CGAACCAGAT 
CCTCCCGGCG AACGCTATAT GCGATTTGGC CAGCCCTCTT CCAGCATCCT GGAGACTATC 
CTCACCAGCC AACATCGCTT GGAGGCGGCA GCTGCTGCGG CAAATGCCTG CCGACAGGCC 
AATGCCGCCA CACCACCGCC CACATCGCCC ACGGAGATGG CCTACAGCTA CAAGAAATCC 
CAGCGGTACG GTAATGCCGT GAGTCCGGAT AGTAGTTCCA ATCTGGGACA GAATCCCGAG 
CAGCTTTCCT CGTCGGCAGT GGTGGTCGGC GAACAGGAAA TGACCAGAGC CACCATGATC 
AAGGGAGAGT GCTCACCACC ACCGCCTAGC CACCATCATC ACAATGTCAT CTTCTCGCCC 
TCGAGACATG CTGCTTATCT GGGAGCCGGC GAAGCGGGTG GCCACTCACC GAGTCCGGGT 
TATCCTGGCT ATCCCCACTA TGGAGCTGCA GCCACCTCGA CCTTCCACTC ACCACCACAT 
AGTTCCCATT CCCCGTTCGA TCGCCAGTCG AATGCATCCA GTGGTGCGGG TTCGGCCACA 
AATCTGCATT TATTGCAAAC CAGCACCCAA ATGCTGAACC ATCCGCTGAT GCAGCCCCTC 
ACACCCCTGC AGCGTTTGTC CCCGCTGAGG ATATCCCCAC CCAGCAGCCT CAGTCCTGAT 
GGCAACTCGT GTCCCCGCAG CGGCAGCCCC TTGAGTCCCA ATTCCTTGGC CTCCAGAGGC 
TACCGATCGT TGCCTTATCC GCTCAAGAAG AAGGATGGCA AGATGCACTA CGAGTGCAAT 
GTGTGCTGCA AGACTTTTGG CCAATTGAGC AACCTCAAGG TGCACCTGAG AACCCATTCC 
GGCGAAAGGC CCTTCAAGTG CAATGTGTGC ACGAAGAGCT TCACCCAGTT GGCGCACCTC 
CAGAAGCATC ATCTGGTGCA TACGGGCGAG AAACCGCATC AGTGCGACAT CTGCAAGAAG 
CGTTTCTCGA GCACCTCCAA CCTGAAGACC CATTTGAGGC TGCACAGTGG TCAGAAACCA 
TATGCCTGTG ATCTCTGCCC GCAGAAGTTC ACACAGTTTG TGCATCTGAA GTTGCACAAG 
AGACTGCACA CGAACGACAG GCCCTATGTG TGCCAGGGTT GCGACAAGAA GTACATCAGT 
GCATCCGGCT TGAGGACCCA CTGGAAGACC ACCAGCTGCA AGCCCAACAA TCTGGAGGAG 
GAGCTGGCCA TGGCAGCAGC GGCAACATCG GAGTGTTTGG ATAAAGACCA TCCCGAACCG 
GACTCCCGAG AAGCCTACGA GCAACTGACG CAGCACATGC ATCCTGCTGT GCATCCGGGA 
TTGAGGCACC TCTCGAGTGG GGGTCAATCG CCACCGCGCC TGATACCCCT GGGCAATCAC 
ATGGCGCCAC AACAGCAGTC GCATCAGCAG CAGCAACAGC AGCATCAGCA GCAGGGAGTG 
CCACCGCCCC ACTTACTGAT GACCCAGCAT TCGGTGGGTC CGGCACCCAT GCTGCTGACC 
ACCGCCAGCC AGTTGCCACC ACCGCCGCCA CATCATCAGC AGAACTCGCC CAGCCGTCTG 
CTGCAACATG GCCACGCACA TCCGTTGCAG ATGCAACAGC AACAGCAGCA GCAACAGCAG 
CACTCGCCCA AGGGACTCAA ATCCCTGCCG GAATCGGGGG TGTATCTGCA TGGACAGCAT 
GTGCAGGCCC AGGAGAGCAG ACCATCGGTC ATTGAGTCCT CCAACCAGCC CATGATCATC 
GAATGCACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_647982.1
CDS	626..4237
Translation	MHGHHNHNHLPQQQPQNPQEQQSGAIMKAANQPHHPAHSHPLNHPHPHQHAQPHPHPHPHPHHKLSARRARSRSRSRSPTPDGNQDYDVTRMREEDFERLAVYLVPDVQAERGLPNRADKTLPRSLTLKSSMVYSTPNVKTEGVWSSGVIPRGTRFGPFEGIPTSNYPNDKNKARYFWRIFKDDDYYYLDGSDRSQSNWMRYVASAYSLSVMNLVACQHQENIYFYTTRDILPNEELMVWYCKDFASRLGYDVDPETTIFGACKQAVEAEEEADEEEEEAEGEDGKPRYSMPAPEIPPDVAVSHITYVMGLHLPVGAGNGPANGSVAGSVSGATPPPPTATPCCRRSSPPAHVSTTTSTCNAHHPHIQHGRHASVIIGQDRSPMASSDKDTAGSPLSGLDHQMTPQDGSVRSVRSDEGYHSNECHEDGLTPPEDSSDSESEHNYVLDCSKKAIAPKETVIAQAQKPSSSPPAVVMANTTTNSMISHSSPTATPICETDKNEYRKFKVKMPLKYEFKNKTCVKQEPSLKEVDQEMSLPQEEEEDQVMHPEPDSICPSTTTHLGDEHMLMMERERERERERIQEREPSNQQPASSTVIVLEHNSGGQARTIVPLSKPYYEPDPPGERYMRFGQPSSSILETILTSQHRLEAAAAAANACRQANAATPPPTSPTEMAYSYKKSQRYGNAVSPDSSSNLGQNPEQLSSSAVVVGEQEMTRATMIKGECSPPPPSHHHHNVIFSPSRHAAYLGAGEAGGHSPSPGYPGYPHYGAAATSTFHSPPHSSHSPFDRQSNASSGAGSATNLHLLQTSTQMLNHPLMQPLTPLQRLSPLRISPPSSLSPDGNSCPRSGSPLSPNSLASRGYRSLPYPLKKKDGKMHYECNVCCKTFGQLSNLKVHLRTHSGERPFKCNVCTKSFTQLAHLQKHHLVHTGEKPHQCDICKKRFSSTSNLKTHLRLHSGQKPYACDLCPQKFTQFVHLKLHKRLHTNDRPYVCQGCDKKYISASGLRTHWKTTSCKPNNLEEELAMAAAATSECLDKDHPEPDSREAYEQLTQHMHPAVHPGLRHLSSGGQSPPRLIPLGNHMAPQQQSHQQQQQQHQQQGVPPPHLLMTQHSVGPAPMLLTTASQLPPPPPHHQQNSPSRLLQHGHAHPLQMQQQQQQQQQHSPKGLKSLPESGVYLHGQHVQAQESRPSVIESSNQPMIIECT

Target ORF information:

RefSeq Version	NM_139725.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Blimp-1, transcript variant A (Blimp-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139725.3

ORF Insert Sequence:

ATGCATGGAC ATCACAACCA CAACCATCTG CCGCAGCAGC AGCCACAAAA TCCGCAGGAG 
CAGCAGTCGG GTGCCATCAT GAAGGCAGCC AACCAGCCCC ACCATCCCGC CCACAGCCAT 
CCGCTCAATC ACCCACATCC GCACCAGCAT GCCCAGCCAC ATCCGCATCC ACATCCCCAT 
CCGCATCACA AGCTGTCGGC GAGAAGGGCG CGCAGTCGCA GTCGAAGTCG CAGTCCGACG 
CCGGATGGCA ATCAGGACTA CGATGTGACC AGGATGCGGG AGGAGGACTT CGAGCGGCTG 
GCCGTCTACC TGGTGCCGGA TGTCCAGGCG GAGCGGGGAC TGCCCAATCG GGCGGACAAG 
ACGCTGCCGC GCAGCCTGAC GCTCAAGTCA TCCATGGTCT ACTCAACGCC AAATGTTAAG 
ACTGAAGGAG TTTGGAGCAG CGGGGTCATC CCACGTGGCA CTCGCTTTGG CCCATTCGAA 
GGCATTCCAA CCTCTAATTA TCCCAATGAT AAGAATAAGG CGCGCTATTT CTGGCGGATC 
TTCAAGGACG ATGACTACTA TTACTTGGAT GGTTCCGATC GCTCGCAGTC CAATTGGATG 
CGCTATGTGG CCTCCGCTTA TAGTTTATCC GTAATGAACC TGGTGGCGTG CCAGCACCAG 
GAGAACATCT ATTTCTACAC CACCCGCGAC ATTCTGCCCA ACGAGGAGCT AATGGTTTGG 
TACTGCAAGG ACTTCGCCAG TCGCCTGGGC TACGATGTGG ATCCTGAGAC GACGATCTTC 
GGCGCATGCA AGCAGGCTGT GGAAGCGGAG GAGGAGGCGG ACGAGGAGGA GGAAGAGGCG 
GAGGGAGAGG ACGGAAAGCC GCGATACTCG ATGCCAGCTC CGGAAATTCC ACCCGATGTG 
GCCGTGAGTC ACATAACCTA CGTCATGGGT CTCCATCTGC CTGTGGGAGC GGGAAATGGT 
CCGGCGAATG GTTCGGTTGC GGGTTCCGTT TCCGGTGCCA CACCTCCACC ACCGACTGCC 
ACGCCATGCT GCCGCCGCTC CAGTCCACCC GCCCACGTCA GCACCACCAC CTCCACTTGC 
AACGCCCACC ACCCGCACAT CCAGCATGGT CGACATGCCT CCGTAATCAT CGGCCAGGAT 
CGATCGCCTA TGGCCAGCAG TGACAAGGAT ACGGCGGGAT CTCCCCTTTC CGGTCTGGAC 
CATCAGATGA CCCCGCAGGA TGGCAGCGTG AGGTCTGTGA GATCGGACGA GGGCTACCAC 
AGCAACGAGT GCCACGAGGA TGGACTCACA CCACCCGAGG ATTCCAGCGA CAGCGAGAGT 
GAACATAACT ACGTGCTGGA CTGCTCGAAG AAGGCAATTG CTCCCAAAGA GACAGTGATT 
GCCCAGGCCC AAAAGCCCAG CAGTTCTCCA CCGGCAGTGG TAATGGCCAA CACGACTACC 
AACTCGATGA TTAGCCACAG TTCACCCACT GCGACGCCCA TTTGCGAAAC GGACAAGAAT 
GAGTATAGAA AGTTCAAGGT GAAAATGCCG CTGAAGTACG AGTTCAAGAA CAAGACCTGC 
GTAAAACAGG AGCCAAGCCT TAAGGAAGTG GACCAGGAGA TGTCCTTGCC GCAGGAGGAG 
GAGGAGGATC AGGTGATGCA TCCGGAACCC GATTCAATTT GCCCATCGAC CACCACCCAT 
CTGGGCGATG AGCACATGCT GATGATGGAG CGGGAACGGG AAAGGGAGCG CGAAAGGATC 
CAGGAGAGGG AACCATCCAA CCAGCAGCCC GCCTCCTCCA CGGTCATCGT ACTGGAACAC 
AACTCTGGCG GTCAAGCCCG CACCATTGTC CCACTGAGTA AACCCTACTA CGAACCAGAT 
CCTCCCGGCG AACGCTATAT GCGATTTGGC CAGCCCTCTT CCAGCATCCT GGAGACTATC 
CTCACCAGCC AACATCGCTT GGAGGCGGCA GCTGCTGCGG CAAATGCCTG CCGACAGGCC 
AATGCCGCCA CACCACCGCC CACATCGCCC ACGGAGATGG CCTACAGCTA CAAGAAATCC 
CAGCGGTACG GTAATGCCGT GAGTCCGGAT AGTAGTTCCA ATCTGGGACA GAATCCCGAG 
CAGCTTTCCT CGTCGGCAGT GGTGGTCGGC GAACAGGAAA TGACCAGAGC CACCATGATC 
AAGGGAGAGT GCTCACCACC ACCGCCTAGC CACCATCATC ACAATGTCAT CTTCTCGCCC 
TCGAGACATG CTGCTTATCT GGGAGCCGGC GAAGCGGGTG GCCACTCACC GAGTCCGGGT 
TATCCTGGCT ATCCCCACTA TGGAGCTGCA GCCACCTCGA CCTTCCACTC ACCACCACAT 
AGTTCCCATT CCCCGTTCGA TCGCCAGTCG AATGCATCCA GTGGTGCGGG TTCGGCCACA 
AATCTGCATT TATTGCAAAC CAGCACCCAA ATGCTGAACC ATCCGCTGAT GCAGCCCCTC 
ACACCCCTGC AGCGTTTGTC CCCGCTGAGG ATATCCCCAC CCAGCAGCCT CAGTCCTGAT 
GGCAACTCGT GTCCCCGCAG CGGCAGCCCC TTGAGTCCCA ATTCCTTGGC CTCCAGAGGC 
TACCGATCGT TGCCTTATCC GCTCAAGAAG AAGGATGGCA AGATGCACTA CGAGTGCAAT 
GTGTGCTGCA AGACTTTTGG CCAATTGAGC AACCTCAAGG TGCACCTGAG AACCCATTCC 
GGCGAAAGGC CCTTCAAGTG CAATGTGTGC ACGAAGAGCT TCACCCAGTT GGCGCACCTC 
CAGAAGCATC ATCTGGTGCA TACGGGCGAG AAACCGCATC AGTGCGACAT CTGCAAGAAG 
CGTTTCTCGA GCACCTCCAA CCTGAAGACC CATTTGAGGC TGCACAGTGG TCAGAAACCA 
TATGCCTGTG ATCTCTGCCC GCAGAAGTTC ACACAGTTTG TGCATCTGAA GTTGCACAAG 
AGACTGCACA CGAACGACAG GCCCTATGTG TGCCAGGGTT GCGACAAGAA GTACATCAGT 
GCATCCGGCT TGAGGACCCA CTGGAAGACC ACCAGCTGCA AGCCCAACAA TCTGGAGGAG 
GAGCTGGCCA TGGCAGCAGC GGCAACATCG GAGTGTTTGG ATAAAGACCA TCCCGAACCG 
GACTCCCGAG AAGCCTACGA GCAACTGACG CAGCACATGC ATCCTGCTGT GCATCCGGGA 
TTGAGGCACC TCTCGAGTGG GGGTCAATCG CCACCGCGCC TGATACCCCT GGGCAATCAC 
ATGGCGCCAC AACAGCAGTC GCATCAGCAG CAGCAACAGC AGCATCAGCA GCAGGGAGTG 
CCACCGCCCC ACTTACTGAT GACCCAGCAT TCGGTGGGTC CGGCACCCAT GCTGCTGACC 
ACCGCCAGCC AGTTGCCACC ACCGCCGCCA CATCATCAGC AGAACTCGCC CAGCCGTCTG 
CTGCAACATG GCCACGCACA TCCGTTGCAG ATGCAACAGC AACAGCAGCA GCAACAGCAG 
CACTCGCCCA AGGGACTCAA ATCCCTGCCG GAATCGGGGG TGTATCTGCA TGGACAGCAT 
GTGCAGGCCC AGGAGAGCAG ACCATCGGTC ATTGAGTCCT CCAACCAGCC CATGATCATC 
GAATGCACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Blimp-1 ( NM_001274515.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261444.1 Drosophila melanogaster Blimp-1, transcript variant D (Blimp-1), mRNA.

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CloneID	OFa17613
Clone ID Related Accession (Same CDS sequence)	NM_001300016.1 , NM_001274516.1 , NM_139725.3 , NM_001274515.1
Accession Version	NM_001274515.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Blimp-1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATGGAC ATCACAACCA CAACCATCTG CCGCAGCAGC AGCCACAAAA TCCGCAGGAG 
CAGCAGTCGG GTGCCATCAT GAAGGCAGCC AACCAGCCCC ACCATCCCGC CCACAGCCAT 
CCGCTCAATC ACCCACATCC GCACCAGCAT GCCCAGCCAC ATCCGCATCC ACATCCCCAT 
CCGCATCACA AGCTGTCGGC GAGAAGGGCG CGCAGTCGCA GTCGAAGTCG CAGTCCGACG 
CCGGATGGCA ATCAGGACTA CGATGTGACC AGGATGCGGG AGGAGGACTT CGAGCGGCTG 
GCCGTCTACC TGGTGCCGGA TGTCCAGGCG GAGCGGGGAC TGCCCAATCG GGCGGACAAG 
ACGCTGCCGC GCAGCCTGAC GCTCAAGTCA TCCATGGTCT ACTCAACGCC AAATGTTAAG 
ACTGAAGGAG TTTGGAGCAG CGGGGTCATC CCACGTGGCA CTCGCTTTGG CCCATTCGAA 
GGCATTCCAA CCTCTAATTA TCCCAATGAT AAGAATAAGG CGCGCTATTT CTGGCGGATC 
TTCAAGGACG ATGACTACTA TTACTTGGAT GGTTCCGATC GCTCGCAGTC CAATTGGATG 
CGCTATGTGG CCTCCGCTTA TAGTTTATCC GTAATGAACC TGGTGGCGTG CCAGCACCAG 
GAGAACATCT ATTTCTACAC CACCCGCGAC ATTCTGCCCA ACGAGGAGCT AATGGTTTGG 
TACTGCAAGG ACTTCGCCAG TCGCCTGGGC TACGATGTGG ATCCTGAGAC GACGATCTTC 
GGCGCATGCA AGCAGGCTGT GGAAGCGGAG GAGGAGGCGG ACGAGGAGGA GGAAGAGGCG 
GAGGGAGAGG ACGGAAAGCC GCGATACTCG ATGCCAGCTC CGGAAATTCC ACCCGATGTG 
GCCGTGAGTC ACATAACCTA CGTCATGGGT CTCCATCTGC CTGTGGGAGC GGGAAATGGT 
CCGGCGAATG GTTCGGTTGC GGGTTCCGTT TCCGGTGCCA CACCTCCACC ACCGACTGCC 
ACGCCATGCT GCCGCCGCTC CAGTCCACCC GCCCACGTCA GCACCACCAC CTCCACTTGC 
AACGCCCACC ACCCGCACAT CCAGCATGGT CGACATGCCT CCGTAATCAT CGGCCAGGAT 
CGATCGCCTA TGGCCAGCAG TGACAAGGAT ACGGCGGGAT CTCCCCTTTC CGGTCTGGAC 
CATCAGATGA CCCCGCAGGA TGGCAGCGTG AGGTCTGTGA GATCGGACGA GGGCTACCAC 
AGCAACGAGT GCCACGAGGA TGGACTCACA CCACCCGAGG ATTCCAGCGA CAGCGAGAGT 
GAACATAACT ACGTGCTGGA CTGCTCGAAG AAGGCAATTG CTCCCAAAGA GACAGTGATT 
GCCCAGGCCC AAAAGCCCAG CAGTTCTCCA CCGGCAGTGG TAATGGCCAA CACGACTACC 
AACTCGATGA TTAGCCACAG TTCACCCACT GCGACGCCCA TTTGCGAAAC GGACAAGAAT 
GAGTATAGAA AGTTCAAGGT GAAAATGCCG CTGAAGTACG AGTTCAAGAA CAAGACCTGC 
GTAAAACAGG AGCCAAGCCT TAAGGAAGTG GACCAGGAGA TGTCCTTGCC GCAGGAGGAG 
GAGGAGGATC AGGTGATGCA TCCGGAACCC GATTCAATTT GCCCATCGAC CACCACCCAT 
CTGGGCGATG AGCACATGCT GATGATGGAG CGGGAACGGG AAAGGGAGCG CGAAAGGATC 
CAGGAGAGGG AACCATCCAA CCAGCAGCCC GCCTCCTCCA CGGTCATCGT ACTGGAACAC 
AACTCTGGCG GTCAAGCCCG CACCATTGTC CCACTGAGTA AACCCTACTA CGAACCAGAT 
CCTCCCGGCG AACGCTATAT GCGATTTGGC CAGCCCTCTT CCAGCATCCT GGAGACTATC 
CTCACCAGCC AACATCGCTT GGAGGCGGCA GCTGCTGCGG CAAATGCCTG CCGACAGGCC 
AATGCCGCCA CACCACCGCC CACATCGCCC ACGGAGATGG CCTACAGCTA CAAGAAATCC 
CAGCGGTACG GTAATGCCGT GAGTCCGGAT AGTAGTTCCA ATCTGGGACA GAATCCCGAG 
CAGCTTTCCT CGTCGGCAGT GGTGGTCGGC GAACAGGAAA TGACCAGAGC CACCATGATC 
AAGGGAGAGT GCTCACCACC ACCGCCTAGC CACCATCATC ACAATGTCAT CTTCTCGCCC 
TCGAGACATG CTGCTTATCT GGGAGCCGGC GAAGCGGGTG GCCACTCACC GAGTCCGGGT 
TATCCTGGCT ATCCCCACTA TGGAGCTGCA GCCACCTCGA CCTTCCACTC ACCACCACAT 
AGTTCCCATT CCCCGTTCGA TCGCCAGTCG AATGCATCCA GTGGTGCGGG TTCGGCCACA 
AATCTGCATT TATTGCAAAC CAGCACCCAA ATGCTGAACC ATCCGCTGAT GCAGCCCCTC 
ACACCCCTGC AGCGTTTGTC CCCGCTGAGG ATATCCCCAC CCAGCAGCCT CAGTCCTGAT 
GGCAACTCGT GTCCCCGCAG CGGCAGCCCC TTGAGTCCCA ATTCCTTGGC CTCCAGAGGC 
TACCGATCGT TGCCTTATCC GCTCAAGAAG AAGGATGGCA AGATGCACTA CGAGTGCAAT 
GTGTGCTGCA AGACTTTTGG CCAATTGAGC AACCTCAAGG TGCACCTGAG AACCCATTCC 
GGCGAAAGGC CCTTCAAGTG CAATGTGTGC ACGAAGAGCT TCACCCAGTT GGCGCACCTC 
CAGAAGCATC ATCTGGTGCA TACGGGCGAG AAACCGCATC AGTGCGACAT CTGCAAGAAG 
CGTTTCTCGA GCACCTCCAA CCTGAAGACC CATTTGAGGC TGCACAGTGG TCAGAAACCA 
TATGCCTGTG ATCTCTGCCC GCAGAAGTTC ACACAGTTTG TGCATCTGAA GTTGCACAAG 
AGACTGCACA CGAACGACAG GCCCTATGTG TGCCAGGGTT GCGACAAGAA GTACATCAGT 
GCATCCGGCT TGAGGACCCA CTGGAAGACC ACCAGCTGCA AGCCCAACAA TCTGGAGGAG 
GAGCTGGCCA TGGCAGCAGC GGCAACATCG GAGTGTTTGG ATAAAGACCA TCCCGAACCG 
GACTCCCGAG AAGCCTACGA GCAACTGACG CAGCACATGC ATCCTGCTGT GCATCCGGGA 
TTGAGGCACC TCTCGAGTGG GGGTCAATCG CCACCGCGCC TGATACCCCT GGGCAATCAC 
ATGGCGCCAC AACAGCAGTC GCATCAGCAG CAGCAACAGC AGCATCAGCA GCAGGGAGTG 
CCACCGCCCC ACTTACTGAT GACCCAGCAT TCGGTGGGTC CGGCACCCAT GCTGCTGACC 
ACCGCCAGCC AGTTGCCACC ACCGCCGCCA CATCATCAGC AGAACTCGCC CAGCCGTCTG 
CTGCAACATG GCCACGCACA TCCGTTGCAG ATGCAACAGC AACAGCAGCA GCAACAGCAG 
CACTCGCCCA AGGGACTCAA ATCCCTGCCG GAATCGGGGG TGTATCTGCA TGGACAGCAT 
GTGCAGGCCC AGGAGAGCAG ACCATCGGTC ATTGAGTCCT CCAACCAGCC CATGATCATC 
GAATGCACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261444.1
CDS	459..4070
Translation	MHGHHNHNHLPQQQPQNPQEQQSGAIMKAANQPHHPAHSHPLNHPHPHQHAQPHPHPHPHPHHKLSARRARSRSRSRSPTPDGNQDYDVTRMREEDFERLAVYLVPDVQAERGLPNRADKTLPRSLTLKSSMVYSTPNVKTEGVWSSGVIPRGTRFGPFEGIPTSNYPNDKNKARYFWRIFKDDDYYYLDGSDRSQSNWMRYVASAYSLSVMNLVACQHQENIYFYTTRDILPNEELMVWYCKDFASRLGYDVDPETTIFGACKQAVEAEEEADEEEEEAEGEDGKPRYSMPAPEIPPDVAVSHITYVMGLHLPVGAGNGPANGSVAGSVSGATPPPPTATPCCRRSSPPAHVSTTTSTCNAHHPHIQHGRHASVIIGQDRSPMASSDKDTAGSPLSGLDHQMTPQDGSVRSVRSDEGYHSNECHEDGLTPPEDSSDSESEHNYVLDCSKKAIAPKETVIAQAQKPSSSPPAVVMANTTTNSMISHSSPTATPICETDKNEYRKFKVKMPLKYEFKNKTCVKQEPSLKEVDQEMSLPQEEEEDQVMHPEPDSICPSTTTHLGDEHMLMMERERERERERIQEREPSNQQPASSTVIVLEHNSGGQARTIVPLSKPYYEPDPPGERYMRFGQPSSSILETILTSQHRLEAAAAAANACRQANAATPPPTSPTEMAYSYKKSQRYGNAVSPDSSSNLGQNPEQLSSSAVVVGEQEMTRATMIKGECSPPPPSHHHHNVIFSPSRHAAYLGAGEAGGHSPSPGYPGYPHYGAAATSTFHSPPHSSHSPFDRQSNASSGAGSATNLHLLQTSTQMLNHPLMQPLTPLQRLSPLRISPPSSLSPDGNSCPRSGSPLSPNSLASRGYRSLPYPLKKKDGKMHYECNVCCKTFGQLSNLKVHLRTHSGERPFKCNVCTKSFTQLAHLQKHHLVHTGEKPHQCDICKKRFSSTSNLKTHLRLHSGQKPYACDLCPQKFTQFVHLKLHKRLHTNDRPYVCQGCDKKYISASGLRTHWKTTSCKPNNLEEELAMAAAATSECLDKDHPEPDSREAYEQLTQHMHPAVHPGLRHLSSGGQSPPRLIPLGNHMAPQQQSHQQQQQQHQQQGVPPPHLLMTQHSVGPAPMLLTTASQLPPPPPHHQQNSPSRLLQHGHAHPLQMQQQQQQQQQHSPKGLKSLPESGVYLHGQHVQAQESRPSVIESSNQPMIIECT

Target ORF information:

RefSeq Version	NM_001274515.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Blimp-1, transcript variant D (Blimp-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274515.1

ORF Insert Sequence:

ATGCATGGAC ATCACAACCA CAACCATCTG CCGCAGCAGC AGCCACAAAA TCCGCAGGAG 
CAGCAGTCGG GTGCCATCAT GAAGGCAGCC AACCAGCCCC ACCATCCCGC CCACAGCCAT 
CCGCTCAATC ACCCACATCC GCACCAGCAT GCCCAGCCAC ATCCGCATCC ACATCCCCAT 
CCGCATCACA AGCTGTCGGC GAGAAGGGCG CGCAGTCGCA GTCGAAGTCG CAGTCCGACG 
CCGGATGGCA ATCAGGACTA CGATGTGACC AGGATGCGGG AGGAGGACTT CGAGCGGCTG 
GCCGTCTACC TGGTGCCGGA TGTCCAGGCG GAGCGGGGAC TGCCCAATCG GGCGGACAAG 
ACGCTGCCGC GCAGCCTGAC GCTCAAGTCA TCCATGGTCT ACTCAACGCC AAATGTTAAG 
ACTGAAGGAG TTTGGAGCAG CGGGGTCATC CCACGTGGCA CTCGCTTTGG CCCATTCGAA 
GGCATTCCAA CCTCTAATTA TCCCAATGAT AAGAATAAGG CGCGCTATTT CTGGCGGATC 
TTCAAGGACG ATGACTACTA TTACTTGGAT GGTTCCGATC GCTCGCAGTC CAATTGGATG 
CGCTATGTGG CCTCCGCTTA TAGTTTATCC GTAATGAACC TGGTGGCGTG CCAGCACCAG 
GAGAACATCT ATTTCTACAC CACCCGCGAC ATTCTGCCCA ACGAGGAGCT AATGGTTTGG 
TACTGCAAGG ACTTCGCCAG TCGCCTGGGC TACGATGTGG ATCCTGAGAC GACGATCTTC 
GGCGCATGCA AGCAGGCTGT GGAAGCGGAG GAGGAGGCGG ACGAGGAGGA GGAAGAGGCG 
GAGGGAGAGG ACGGAAAGCC GCGATACTCG ATGCCAGCTC CGGAAATTCC ACCCGATGTG 
GCCGTGAGTC ACATAACCTA CGTCATGGGT CTCCATCTGC CTGTGGGAGC GGGAAATGGT 
CCGGCGAATG GTTCGGTTGC GGGTTCCGTT TCCGGTGCCA CACCTCCACC ACCGACTGCC 
ACGCCATGCT GCCGCCGCTC CAGTCCACCC GCCCACGTCA GCACCACCAC CTCCACTTGC 
AACGCCCACC ACCCGCACAT CCAGCATGGT CGACATGCCT CCGTAATCAT CGGCCAGGAT 
CGATCGCCTA TGGCCAGCAG TGACAAGGAT ACGGCGGGAT CTCCCCTTTC CGGTCTGGAC 
CATCAGATGA CCCCGCAGGA TGGCAGCGTG AGGTCTGTGA GATCGGACGA GGGCTACCAC 
AGCAACGAGT GCCACGAGGA TGGACTCACA CCACCCGAGG ATTCCAGCGA CAGCGAGAGT 
GAACATAACT ACGTGCTGGA CTGCTCGAAG AAGGCAATTG CTCCCAAAGA GACAGTGATT 
GCCCAGGCCC AAAAGCCCAG CAGTTCTCCA CCGGCAGTGG TAATGGCCAA CACGACTACC 
AACTCGATGA TTAGCCACAG TTCACCCACT GCGACGCCCA TTTGCGAAAC GGACAAGAAT 
GAGTATAGAA AGTTCAAGGT GAAAATGCCG CTGAAGTACG AGTTCAAGAA CAAGACCTGC 
GTAAAACAGG AGCCAAGCCT TAAGGAAGTG GACCAGGAGA TGTCCTTGCC GCAGGAGGAG 
GAGGAGGATC AGGTGATGCA TCCGGAACCC GATTCAATTT GCCCATCGAC CACCACCCAT 
CTGGGCGATG AGCACATGCT GATGATGGAG CGGGAACGGG AAAGGGAGCG CGAAAGGATC 
CAGGAGAGGG AACCATCCAA CCAGCAGCCC GCCTCCTCCA CGGTCATCGT ACTGGAACAC 
AACTCTGGCG GTCAAGCCCG CACCATTGTC CCACTGAGTA AACCCTACTA CGAACCAGAT 
CCTCCCGGCG AACGCTATAT GCGATTTGGC CAGCCCTCTT CCAGCATCCT GGAGACTATC 
CTCACCAGCC AACATCGCTT GGAGGCGGCA GCTGCTGCGG CAAATGCCTG CCGACAGGCC 
AATGCCGCCA CACCACCGCC CACATCGCCC ACGGAGATGG CCTACAGCTA CAAGAAATCC 
CAGCGGTACG GTAATGCCGT GAGTCCGGAT AGTAGTTCCA ATCTGGGACA GAATCCCGAG 
CAGCTTTCCT CGTCGGCAGT GGTGGTCGGC GAACAGGAAA TGACCAGAGC CACCATGATC 
AAGGGAGAGT GCTCACCACC ACCGCCTAGC CACCATCATC ACAATGTCAT CTTCTCGCCC 
TCGAGACATG CTGCTTATCT GGGAGCCGGC GAAGCGGGTG GCCACTCACC GAGTCCGGGT 
TATCCTGGCT ATCCCCACTA TGGAGCTGCA GCCACCTCGA CCTTCCACTC ACCACCACAT 
AGTTCCCATT CCCCGTTCGA TCGCCAGTCG AATGCATCCA GTGGTGCGGG TTCGGCCACA 
AATCTGCATT TATTGCAAAC CAGCACCCAA ATGCTGAACC ATCCGCTGAT GCAGCCCCTC 
ACACCCCTGC AGCGTTTGTC CCCGCTGAGG ATATCCCCAC CCAGCAGCCT CAGTCCTGAT 
GGCAACTCGT GTCCCCGCAG CGGCAGCCCC TTGAGTCCCA ATTCCTTGGC CTCCAGAGGC 
TACCGATCGT TGCCTTATCC GCTCAAGAAG AAGGATGGCA AGATGCACTA CGAGTGCAAT 
GTGTGCTGCA AGACTTTTGG CCAATTGAGC AACCTCAAGG TGCACCTGAG AACCCATTCC 
GGCGAAAGGC CCTTCAAGTG CAATGTGTGC ACGAAGAGCT TCACCCAGTT GGCGCACCTC 
CAGAAGCATC ATCTGGTGCA TACGGGCGAG AAACCGCATC AGTGCGACAT CTGCAAGAAG 
CGTTTCTCGA GCACCTCCAA CCTGAAGACC CATTTGAGGC TGCACAGTGG TCAGAAACCA 
TATGCCTGTG ATCTCTGCCC GCAGAAGTTC ACACAGTTTG TGCATCTGAA GTTGCACAAG 
AGACTGCACA CGAACGACAG GCCCTATGTG TGCCAGGGTT GCGACAAGAA GTACATCAGT 
GCATCCGGCT TGAGGACCCA CTGGAAGACC ACCAGCTGCA AGCCCAACAA TCTGGAGGAG 
GAGCTGGCCA TGGCAGCAGC GGCAACATCG GAGTGTTTGG ATAAAGACCA TCCCGAACCG 
GACTCCCGAG AAGCCTACGA GCAACTGACG CAGCACATGC ATCCTGCTGT GCATCCGGGA 
TTGAGGCACC TCTCGAGTGG GGGTCAATCG CCACCGCGCC TGATACCCCT GGGCAATCAC 
ATGGCGCCAC AACAGCAGTC GCATCAGCAG CAGCAACAGC AGCATCAGCA GCAGGGAGTG 
CCACCGCCCC ACTTACTGAT GACCCAGCAT TCGGTGGGTC CGGCACCCAT GCTGCTGACC 
ACCGCCAGCC AGTTGCCACC ACCGCCGCCA CATCATCAGC AGAACTCGCC CAGCCGTCTG 
CTGCAACATG GCCACGCACA TCCGTTGCAG ATGCAACAGC AACAGCAGCA GCAACAGCAG 
CACTCGCCCA AGGGACTCAA ATCCCTGCCG GAATCGGGGG TGTATCTGCA TGGACAGCAT 
GTGCAGGCCC AGGAGAGCAG ACCATCGGTC ATTGAGTCCT CCAACCAGCC CATGATCATC 
GAATGCACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Blimp-1 ( NM_001274514.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261443.1 Drosophila melanogaster Blimp-1, transcript variant C (Blimp-1), mRNA.

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CloneID	OFa20109
Clone ID Related Accession (Same CDS sequence)	NM_001274514.1 , NM_001274513.1
Accession Version	NM_001274514.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Blimp-1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATGGAC ATCACAACCA CAACCATCTG CCGCAGCAGC AGCCACAAAA TCCGCAGGAG 
CAGCAGTCGG GTGCCATCAT GAAGGCAGCC AACCAGCCCC ACCATCCCGC CCACAGCCAT 
CCGCTCAATC ACCCACATCC GCACCAGCAT GCCCAGCCAC ATCCGCATCC ACATCCCCAT 
CCGCATCACA AGCTGTCGGC GAGAAGGGCG CGCAGTCGCA GTCGAAGTCG CAGTCCGACG 
CCGGATGGCA ATCAGGACTA CGATGTGACC AGGATGCGGG AGGAGGACTT CGAGCGGCTG 
GCCGTCTACC TGGTGCCGGA TGTCCAGGCG GAGCGGGGAC TGCCCAATCG GGCGGACAAG 
ACGCTGCCGC GCAGCCTGAC GCTCAAGTCA TCCATGGTCT ACTCAACGCC AAATGTTAAG 
ACTGAAGGAG TTTGGAGCAG CGGGGTCATC CCACGTGGCA CTCGCTTTGG CCCATTCGAA 
GGCATTCCAA CCTCTAATTA TCCCAATGAT AAGAATAAGG CGCGCTATTT CTGGCGGGTG 
CAGGGAACAC GCCACATAAC CTACGGGAAT ATTAAGATCT TCAAGGACGA TGACTACTAT 
TACTTGGATG GTTCCGATCG CTCGCAGTCC AATTGGATGC GCTATGTGGC CTCCGCTTAT 
AGTTTATCCG TAATGAACCT GGTGGCGTGC CAGCACCAGG AGAACATCTA TTTCTACACC 
ACCCGCGACA TTCTGCCCAA CGAGGAGCTA ATGGTTTGGT ACTGCAAGGA CTTCGCCAGT 
CGCCTGGGCT ACGATGTGGA TCCTGAGACG ACGATCTTCG GCGCATGCAA GCAGGCTGTG 
GAAGCGGAGG AGGAGGCGGA CGAGGAGGAG GAAGAGGCGG AGGGAGAGGA CGGAAAGCCG 
CGATACTCGA TGCCAGCTCC GGAAATTCCA CCCGATGTGG CCGTGAGTCA CATAACCTAC 
GTCATGGGTC TCCATCTGCC TGTGGGAGCG GGAAATGGTC CGGCGAATGG TTCGGTTGCG 
GGTTCCGTTT CCGGTGCCAC ACCTCCACCA CCGACTGCCA CGCCATGCTG CCGCCGCTCC 
AGTCCACCCG CCCACGTCAG CACCACCACC TCCACTTGCA ACGCCCACCA CCCGCACATC 
CAGCATGGTC GACATGCCTC CGTAATCATC GGCCAGGATC GATCGCCTAT GGCCAGCAGT 
GACAAGGATA CGGCGGGATC TCCCCTTTCC GGTCTGGACC ATCAGATGAC CCCGCAGGAT 
GGCAGCGTGA GGTCTGTGAG ATCGGACGAG GGCTACCACA GCAACGAGTG CCACGAGGAT 
GGACTCACAC CACCCGAGGA TTCCAGCGAC AGCGAGAGTG AACATAACTA CGTGCTGGAC 
TGCTCGAAGA AGGCAATTGC TCCCAAAGAG ACAGTGATTG CCCAGGCCCA AAAGCCCAGC 
AGTTCTCCAC CGGCAGTGGT AATGGCCAAC ACGACTACCA ACTCGATGAT TAGCCACAGT 
TCACCCACTG CGACGCCCAT TTGCGAAACG GACAAGAATG AGTATAGAAA GTTCAAGGTG 
AAAATGCCGC TGAAGTACGA GTTCAAGAAC AAGACCTGCG TAAAACAGGA GCCAAGCCTT 
AAGGAAGTGG ACCAGGAGAT GTCCTTGCCG CAGGAGGAGG AGGAGGATCA GGTGATGCAT 
CCGGAACCCG ATTCAATTTG CCCATCGACC ACCACCCATC TGGGCGATGA GCACATGCTG 
ATGATGGAGC GGGAACGGGA AAGGGAGCGC GAAAGGATCC AGGAGAGGGA ACCATCCAAC 
CAGCAGCCCG CCTCCTCCAC GGTCATCGTA CTGGAACACA ACTCTGGCGG TCAAGCCCGC 
ACCATTGTCC CACTGAGTAA ACCCTACTAC GAACCAGATC CTCCCGGCGA ACGCTATATG 
CGATTTGGCC AGCCCTCTTC CAGCATCCTG GAGACTATCC TCACCAGCCA ACATCGCTTG 
GAGGCGGCAG CTGCTGCGGC AAATGCCTGC CGACAGGCCA ATGCCGCCAC ACCACCGCCC 
ACATCGCCCA CGGAGATGGC CTACAGCTAC AAGAAATCCC AGCGGTACGG TAATGCCGTG 
AGTCCGGATA GTAGTTCCAA TCTGGGACAG AATCCCGAGC AGCTTTCCTC GTCGGCAGTG 
GTGGTCGGCG AACAGGAAAT GACCAGAGCC ACCATGATCA AGGGAGAGTG CTCACCACCA 
CCGCCTAGCC ACCATCATCA CAATGTCATC TTCTCGCCCT CGAGACATGC TGCTTATCTG 
GGAGCCGGCG AAGCGGGTGG CCACTCACCG AGTCCGGGTT ATCCTGGCTA TCCCCACTAT 
GGAGCTGCAG CCACCTCGAC CTTCCACTCA CCACCACATA GTTCCCATTC CCCGTTCGAT 
CGCCAGTCGA ATGCATCCAG TGGTGCGGGT TCGGCCACAA ATCTGCATTT ATTGCAAACC 
AGCACCCAAA TGCTGAACCA TCCGCTGATG CAGCCCCTCA CACCCCTGCA GCGTTTGTCC 
CCGCTGAGGA TATCCCCACC CAGCAGCCTC AGTCCTGATG GCAACTCGTG TCCCCGCAGC 
GGCAGCCCCT TGAGTCCCAA TTCCTTGGCC TCCAGAGGCT ACCGATCGTT GCCTTATCCG 
CTCAAGAAGA AGGATGGCAA GATGCACTAC GAGTGCAATG TGTGCTGCAA GACTTTTGGC 
CAATTGAGCA ACCTCAAGGT GCACCTGAGA ACCCATTCCG GCGAAAGGCC CTTCAAGTGC 
AATGTGTGCA CGAAGAGCTT CACCCAGTTG GCGCACCTCC AGAAGCATCA TCTGGTGCAT 
ACGGGCGAGA AACCGCATCA GTGCGACATC TGCAAGAAGC GTTTCTCGAG CACCTCCAAC 
CTGAAGACCC ATTTGAGGCT GCACAGTGGT CAGAAACCAT ATGCCTGTGA TCTCTGCCCG 
CAGAAGTTCA CACAGTTTGT GCATCTGAAG TTGCACAAGA GACTGCACAC GAACGACAGG 
CCCTATGTGT GCCAGGGTTG CGACAAGAAG TACATCAGTG CATCCGGCTT GAGGACCCAC 
TGGAAGACCA CCAGCTGCAA GCCCAACAAT CTGGAGGAGG AGCTGGCCAT GGCAGCAGCG 
GCAACATCGG AGTGTTTGGA TAAAGACCAT CCCGAACCGG ACTCCCGAGA AGCCTACGAG 
CAACTGACGC AGCACATGCA TCCTGCTGTG CATCCGGGAT TGAGGCACCT CTCGAGTGGG 
GGTCAATCGC CACCGCGCCT GATACCCCTG GGCAATCACA TGGCGCCACA ACAGCAGTCG 
CATCAGCAGC AGCAACAGCA GCATCAGCAG CAGGGAGTGC CACCGCCCCA CTTACTGATG 
ACCCAGCATT CGGTGGGTCC GGCACCCATG CTGCTGACCA CCGCCAGCCA GTTGCCACCA 
CCGCCGCCAC ATCATCAGCA GAACTCGCCC AGCCGTCTGC TGCAACATGG CCACGCACAT 
CCGTTGCAGA TGCAACAGCA ACAGCAGCAG CAACAGCAGC ACTCGCCCAA GGGACTCAAA 
TCCCTGCCGG AATCGGGGGT GTATCTGCAT GGACAGCATG TGCAGGCCCA GGAGAGCAGA 
CCATCGGTCA TTGAGTCCTC CAACCAGCCC ATGATCATCG AATGCACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261443.1
CDS	631..4281
Translation	MHGHHNHNHLPQQQPQNPQEQQSGAIMKAANQPHHPAHSHPLNHPHPHQHAQPHPHPHPHPHHKLSARRARSRSRSRSPTPDGNQDYDVTRMREEDFERLAVYLVPDVQAERGLPNRADKTLPRSLTLKSSMVYSTPNVKTEGVWSSGVIPRGTRFGPFEGIPTSNYPNDKNKARYFWRVQGTRHITYGNIKIFKDDDYYYLDGSDRSQSNWMRYVASAYSLSVMNLVACQHQENIYFYTTRDILPNEELMVWYCKDFASRLGYDVDPETTIFGACKQAVEAEEEADEEEEEAEGEDGKPRYSMPAPEIPPDVAVSHITYVMGLHLPVGAGNGPANGSVAGSVSGATPPPPTATPCCRRSSPPAHVSTTTSTCNAHHPHIQHGRHASVIIGQDRSPMASSDKDTAGSPLSGLDHQMTPQDGSVRSVRSDEGYHSNECHEDGLTPPEDSSDSESEHNYVLDCSKKAIAPKETVIAQAQKPSSSPPAVVMANTTTNSMISHSSPTATPICETDKNEYRKFKVKMPLKYEFKNKTCVKQEPSLKEVDQEMSLPQEEEEDQVMHPEPDSICPSTTTHLGDEHMLMMERERERERERIQEREPSNQQPASSTVIVLEHNSGGQARTIVPLSKPYYEPDPPGERYMRFGQPSSSILETILTSQHRLEAAAAAANACRQANAATPPPTSPTEMAYSYKKSQRYGNAVSPDSSSNLGQNPEQLSSSAVVVGEQEMTRATMIKGECSPPPPSHHHHNVIFSPSRHAAYLGAGEAGGHSPSPGYPGYPHYGAAATSTFHSPPHSSHSPFDRQSNASSGAGSATNLHLLQTSTQMLNHPLMQPLTPLQRLSPLRISPPSSLSPDGNSCPRSGSPLSPNSLASRGYRSLPYPLKKKDGKMHYECNVCCKTFGQLSNLKVHLRTHSGERPFKCNVCTKSFTQLAHLQKHHLVHTGEKPHQCDICKKRFSSTSNLKTHLRLHSGQKPYACDLCPQKFTQFVHLKLHKRLHTNDRPYVCQGCDKKYISASGLRTHWKTTSCKPNNLEEELAMAAAATSECLDKDHPEPDSREAYEQLTQHMHPAVHPGLRHLSSGGQSPPRLIPLGNHMAPQQQSHQQQQQQHQQQGVPPPHLLMTQHSVGPAPMLLTTASQLPPPPPHHQQNSPSRLLQHGHAHPLQMQQQQQQQQQHSPKGLKSLPESGVYLHGQHVQAQESRPSVIESSNQPMIIECT

Target ORF information:

RefSeq Version	NM_001274514.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Blimp-1, transcript variant C (Blimp-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274514.1

ORF Insert Sequence:

ATGCATGGAC ATCACAACCA CAACCATCTG CCGCAGCAGC AGCCACAAAA TCCGCAGGAG 
CAGCAGTCGG GTGCCATCAT GAAGGCAGCC AACCAGCCCC ACCATCCCGC CCACAGCCAT 
CCGCTCAATC ACCCACATCC GCACCAGCAT GCCCAGCCAC ATCCGCATCC ACATCCCCAT 
CCGCATCACA AGCTGTCGGC GAGAAGGGCG CGCAGTCGCA GTCGAAGTCG CAGTCCGACG 
CCGGATGGCA ATCAGGACTA CGATGTGACC AGGATGCGGG AGGAGGACTT CGAGCGGCTG 
GCCGTCTACC TGGTGCCGGA TGTCCAGGCG GAGCGGGGAC TGCCCAATCG GGCGGACAAG 
ACGCTGCCGC GCAGCCTGAC GCTCAAGTCA TCCATGGTCT ACTCAACGCC AAATGTTAAG 
ACTGAAGGAG TTTGGAGCAG CGGGGTCATC CCACGTGGCA CTCGCTTTGG CCCATTCGAA 
GGCATTCCAA CCTCTAATTA TCCCAATGAT AAGAATAAGG CGCGCTATTT CTGGCGGGTG 
CAGGGAACAC GCCACATAAC CTACGGGAAT ATTAAGATCT TCAAGGACGA TGACTACTAT 
TACTTGGATG GTTCCGATCG CTCGCAGTCC AATTGGATGC GCTATGTGGC CTCCGCTTAT 
AGTTTATCCG TAATGAACCT GGTGGCGTGC CAGCACCAGG AGAACATCTA TTTCTACACC 
ACCCGCGACA TTCTGCCCAA CGAGGAGCTA ATGGTTTGGT ACTGCAAGGA CTTCGCCAGT 
CGCCTGGGCT ACGATGTGGA TCCTGAGACG ACGATCTTCG GCGCATGCAA GCAGGCTGTG 
GAAGCGGAGG AGGAGGCGGA CGAGGAGGAG GAAGAGGCGG AGGGAGAGGA CGGAAAGCCG 
CGATACTCGA TGCCAGCTCC GGAAATTCCA CCCGATGTGG CCGTGAGTCA CATAACCTAC 
GTCATGGGTC TCCATCTGCC TGTGGGAGCG GGAAATGGTC CGGCGAATGG TTCGGTTGCG 
GGTTCCGTTT CCGGTGCCAC ACCTCCACCA CCGACTGCCA CGCCATGCTG CCGCCGCTCC 
AGTCCACCCG CCCACGTCAG CACCACCACC TCCACTTGCA ACGCCCACCA CCCGCACATC 
CAGCATGGTC GACATGCCTC CGTAATCATC GGCCAGGATC GATCGCCTAT GGCCAGCAGT 
GACAAGGATA CGGCGGGATC TCCCCTTTCC GGTCTGGACC ATCAGATGAC CCCGCAGGAT 
GGCAGCGTGA GGTCTGTGAG ATCGGACGAG GGCTACCACA GCAACGAGTG CCACGAGGAT 
GGACTCACAC CACCCGAGGA TTCCAGCGAC AGCGAGAGTG AACATAACTA CGTGCTGGAC 
TGCTCGAAGA AGGCAATTGC TCCCAAAGAG ACAGTGATTG CCCAGGCCCA AAAGCCCAGC 
AGTTCTCCAC CGGCAGTGGT AATGGCCAAC ACGACTACCA ACTCGATGAT TAGCCACAGT 
TCACCCACTG CGACGCCCAT TTGCGAAACG GACAAGAATG AGTATAGAAA GTTCAAGGTG 
AAAATGCCGC TGAAGTACGA GTTCAAGAAC AAGACCTGCG TAAAACAGGA GCCAAGCCTT 
AAGGAAGTGG ACCAGGAGAT GTCCTTGCCG CAGGAGGAGG AGGAGGATCA GGTGATGCAT 
CCGGAACCCG ATTCAATTTG CCCATCGACC ACCACCCATC TGGGCGATGA GCACATGCTG 
ATGATGGAGC GGGAACGGGA AAGGGAGCGC GAAAGGATCC AGGAGAGGGA ACCATCCAAC 
CAGCAGCCCG CCTCCTCCAC GGTCATCGTA CTGGAACACA ACTCTGGCGG TCAAGCCCGC 
ACCATTGTCC CACTGAGTAA ACCCTACTAC GAACCAGATC CTCCCGGCGA ACGCTATATG 
CGATTTGGCC AGCCCTCTTC CAGCATCCTG GAGACTATCC TCACCAGCCA ACATCGCTTG 
GAGGCGGCAG CTGCTGCGGC AAATGCCTGC CGACAGGCCA ATGCCGCCAC ACCACCGCCC 
ACATCGCCCA CGGAGATGGC CTACAGCTAC AAGAAATCCC AGCGGTACGG TAATGCCGTG 
AGTCCGGATA GTAGTTCCAA TCTGGGACAG AATCCCGAGC AGCTTTCCTC GTCGGCAGTG 
GTGGTCGGCG AACAGGAAAT GACCAGAGCC ACCATGATCA AGGGAGAGTG CTCACCACCA 
CCGCCTAGCC ACCATCATCA CAATGTCATC TTCTCGCCCT CGAGACATGC TGCTTATCTG 
GGAGCCGGCG AAGCGGGTGG CCACTCACCG AGTCCGGGTT ATCCTGGCTA TCCCCACTAT 
GGAGCTGCAG CCACCTCGAC CTTCCACTCA CCACCACATA GTTCCCATTC CCCGTTCGAT 
CGCCAGTCGA ATGCATCCAG TGGTGCGGGT TCGGCCACAA ATCTGCATTT ATTGCAAACC 
AGCACCCAAA TGCTGAACCA TCCGCTGATG CAGCCCCTCA CACCCCTGCA GCGTTTGTCC 
CCGCTGAGGA TATCCCCACC CAGCAGCCTC AGTCCTGATG GCAACTCGTG TCCCCGCAGC 
GGCAGCCCCT TGAGTCCCAA TTCCTTGGCC TCCAGAGGCT ACCGATCGTT GCCTTATCCG 
CTCAAGAAGA AGGATGGCAA GATGCACTAC GAGTGCAATG TGTGCTGCAA GACTTTTGGC 
CAATTGAGCA ACCTCAAGGT GCACCTGAGA ACCCATTCCG GCGAAAGGCC CTTCAAGTGC 
AATGTGTGCA CGAAGAGCTT CACCCAGTTG GCGCACCTCC AGAAGCATCA TCTGGTGCAT 
ACGGGCGAGA AACCGCATCA GTGCGACATC TGCAAGAAGC GTTTCTCGAG CACCTCCAAC 
CTGAAGACCC ATTTGAGGCT GCACAGTGGT CAGAAACCAT ATGCCTGTGA TCTCTGCCCG 
CAGAAGTTCA CACAGTTTGT GCATCTGAAG TTGCACAAGA GACTGCACAC GAACGACAGG 
CCCTATGTGT GCCAGGGTTG CGACAAGAAG TACATCAGTG CATCCGGCTT GAGGACCCAC 
TGGAAGACCA CCAGCTGCAA GCCCAACAAT CTGGAGGAGG AGCTGGCCAT GGCAGCAGCG 
GCAACATCGG AGTGTTTGGA TAAAGACCAT CCCGAACCGG ACTCCCGAGA AGCCTACGAG 
CAACTGACGC AGCACATGCA TCCTGCTGTG CATCCGGGAT TGAGGCACCT CTCGAGTGGG 
GGTCAATCGC CACCGCGCCT GATACCCCTG GGCAATCACA TGGCGCCACA ACAGCAGTCG 
CATCAGCAGC AGCAACAGCA GCATCAGCAG CAGGGAGTGC CACCGCCCCA CTTACTGATG 
ACCCAGCATT CGGTGGGTCC GGCACCCATG CTGCTGACCA CCGCCAGCCA GTTGCCACCA 
CCGCCGCCAC ATCATCAGCA GAACTCGCCC AGCCGTCTGC TGCAACATGG CCACGCACAT 
CCGTTGCAGA TGCAACAGCA ACAGCAGCAG CAACAGCAGC ACTCGCCCAA GGGACTCAAA 
TCCCTGCCGG AATCGGGGGT GTATCTGCAT GGACAGCATG TGCAGGCCCA GGAGAGCAGA 
CCATCGGTCA TTGAGTCCTC CAACCAGCCC ATGATCATCG AATGCACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Blimp-1 ( NM_001274513.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261442.1 Drosophila melanogaster Blimp-1, transcript variant B (Blimp-1), mRNA.

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CloneID	OFa20109
Clone ID Related Accession (Same CDS sequence)	NM_001274514.1 , NM_001274513.1
Accession Version	NM_001274513.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Blimp-1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATGGAC ATCACAACCA CAACCATCTG CCGCAGCAGC AGCCACAAAA TCCGCAGGAG 
CAGCAGTCGG GTGCCATCAT GAAGGCAGCC AACCAGCCCC ACCATCCCGC CCACAGCCAT 
CCGCTCAATC ACCCACATCC GCACCAGCAT GCCCAGCCAC ATCCGCATCC ACATCCCCAT 
CCGCATCACA AGCTGTCGGC GAGAAGGGCG CGCAGTCGCA GTCGAAGTCG CAGTCCGACG 
CCGGATGGCA ATCAGGACTA CGATGTGACC AGGATGCGGG AGGAGGACTT CGAGCGGCTG 
GCCGTCTACC TGGTGCCGGA TGTCCAGGCG GAGCGGGGAC TGCCCAATCG GGCGGACAAG 
ACGCTGCCGC GCAGCCTGAC GCTCAAGTCA TCCATGGTCT ACTCAACGCC AAATGTTAAG 
ACTGAAGGAG TTTGGAGCAG CGGGGTCATC CCACGTGGCA CTCGCTTTGG CCCATTCGAA 
GGCATTCCAA CCTCTAATTA TCCCAATGAT AAGAATAAGG CGCGCTATTT CTGGCGGGTG 
CAGGGAACAC GCCACATAAC CTACGGGAAT ATTAAGATCT TCAAGGACGA TGACTACTAT 
TACTTGGATG GTTCCGATCG CTCGCAGTCC AATTGGATGC GCTATGTGGC CTCCGCTTAT 
AGTTTATCCG TAATGAACCT GGTGGCGTGC CAGCACCAGG AGAACATCTA TTTCTACACC 
ACCCGCGACA TTCTGCCCAA CGAGGAGCTA ATGGTTTGGT ACTGCAAGGA CTTCGCCAGT 
CGCCTGGGCT ACGATGTGGA TCCTGAGACG ACGATCTTCG GCGCATGCAA GCAGGCTGTG 
GAAGCGGAGG AGGAGGCGGA CGAGGAGGAG GAAGAGGCGG AGGGAGAGGA CGGAAAGCCG 
CGATACTCGA TGCCAGCTCC GGAAATTCCA CCCGATGTGG CCGTGAGTCA CATAACCTAC 
GTCATGGGTC TCCATCTGCC TGTGGGAGCG GGAAATGGTC CGGCGAATGG TTCGGTTGCG 
GGTTCCGTTT CCGGTGCCAC ACCTCCACCA CCGACTGCCA CGCCATGCTG CCGCCGCTCC 
AGTCCACCCG CCCACGTCAG CACCACCACC TCCACTTGCA ACGCCCACCA CCCGCACATC 
CAGCATGGTC GACATGCCTC CGTAATCATC GGCCAGGATC GATCGCCTAT GGCCAGCAGT 
GACAAGGATA CGGCGGGATC TCCCCTTTCC GGTCTGGACC ATCAGATGAC CCCGCAGGAT 
GGCAGCGTGA GGTCTGTGAG ATCGGACGAG GGCTACCACA GCAACGAGTG CCACGAGGAT 
GGACTCACAC CACCCGAGGA TTCCAGCGAC AGCGAGAGTG AACATAACTA CGTGCTGGAC 
TGCTCGAAGA AGGCAATTGC TCCCAAAGAG ACAGTGATTG CCCAGGCCCA AAAGCCCAGC 
AGTTCTCCAC CGGCAGTGGT AATGGCCAAC ACGACTACCA ACTCGATGAT TAGCCACAGT 
TCACCCACTG CGACGCCCAT TTGCGAAACG GACAAGAATG AGTATAGAAA GTTCAAGGTG 
AAAATGCCGC TGAAGTACGA GTTCAAGAAC AAGACCTGCG TAAAACAGGA GCCAAGCCTT 
AAGGAAGTGG ACCAGGAGAT GTCCTTGCCG CAGGAGGAGG AGGAGGATCA GGTGATGCAT 
CCGGAACCCG ATTCAATTTG CCCATCGACC ACCACCCATC TGGGCGATGA GCACATGCTG 
ATGATGGAGC GGGAACGGGA AAGGGAGCGC GAAAGGATCC AGGAGAGGGA ACCATCCAAC 
CAGCAGCCCG CCTCCTCCAC GGTCATCGTA CTGGAACACA ACTCTGGCGG TCAAGCCCGC 
ACCATTGTCC CACTGAGTAA ACCCTACTAC GAACCAGATC CTCCCGGCGA ACGCTATATG 
CGATTTGGCC AGCCCTCTTC CAGCATCCTG GAGACTATCC TCACCAGCCA ACATCGCTTG 
GAGGCGGCAG CTGCTGCGGC AAATGCCTGC CGACAGGCCA ATGCCGCCAC ACCACCGCCC 
ACATCGCCCA CGGAGATGGC CTACAGCTAC AAGAAATCCC AGCGGTACGG TAATGCCGTG 
AGTCCGGATA GTAGTTCCAA TCTGGGACAG AATCCCGAGC AGCTTTCCTC GTCGGCAGTG 
GTGGTCGGCG AACAGGAAAT GACCAGAGCC ACCATGATCA AGGGAGAGTG CTCACCACCA 
CCGCCTAGCC ACCATCATCA CAATGTCATC TTCTCGCCCT CGAGACATGC TGCTTATCTG 
GGAGCCGGCG AAGCGGGTGG CCACTCACCG AGTCCGGGTT ATCCTGGCTA TCCCCACTAT 
GGAGCTGCAG CCACCTCGAC CTTCCACTCA CCACCACATA GTTCCCATTC CCCGTTCGAT 
CGCCAGTCGA ATGCATCCAG TGGTGCGGGT TCGGCCACAA ATCTGCATTT ATTGCAAACC 
AGCACCCAAA TGCTGAACCA TCCGCTGATG CAGCCCCTCA CACCCCTGCA GCGTTTGTCC 
CCGCTGAGGA TATCCCCACC CAGCAGCCTC AGTCCTGATG GCAACTCGTG TCCCCGCAGC 
GGCAGCCCCT TGAGTCCCAA TTCCTTGGCC TCCAGAGGCT ACCGATCGTT GCCTTATCCG 
CTCAAGAAGA AGGATGGCAA GATGCACTAC GAGTGCAATG TGTGCTGCAA GACTTTTGGC 
CAATTGAGCA ACCTCAAGGT GCACCTGAGA ACCCATTCCG GCGAAAGGCC CTTCAAGTGC 
AATGTGTGCA CGAAGAGCTT CACCCAGTTG GCGCACCTCC AGAAGCATCA TCTGGTGCAT 
ACGGGCGAGA AACCGCATCA GTGCGACATC TGCAAGAAGC GTTTCTCGAG CACCTCCAAC 
CTGAAGACCC ATTTGAGGCT GCACAGTGGT CAGAAACCAT ATGCCTGTGA TCTCTGCCCG 
CAGAAGTTCA CACAGTTTGT GCATCTGAAG TTGCACAAGA GACTGCACAC GAACGACAGG 
CCCTATGTGT GCCAGGGTTG CGACAAGAAG TACATCAGTG CATCCGGCTT GAGGACCCAC 
TGGAAGACCA CCAGCTGCAA GCCCAACAAT CTGGAGGAGG AGCTGGCCAT GGCAGCAGCG 
GCAACATCGG AGTGTTTGGA TAAAGACCAT CCCGAACCGG ACTCCCGAGA AGCCTACGAG 
CAACTGACGC AGCACATGCA TCCTGCTGTG CATCCGGGAT TGAGGCACCT CTCGAGTGGG 
GGTCAATCGC CACCGCGCCT GATACCCCTG GGCAATCACA TGGCGCCACA ACAGCAGTCG 
CATCAGCAGC AGCAACAGCA GCATCAGCAG CAGGGAGTGC CACCGCCCCA CTTACTGATG 
ACCCAGCATT CGGTGGGTCC GGCACCCATG CTGCTGACCA CCGCCAGCCA GTTGCCACCA 
CCGCCGCCAC ATCATCAGCA GAACTCGCCC AGCCGTCTGC TGCAACATGG CCACGCACAT 
CCGTTGCAGA TGCAACAGCA ACAGCAGCAG CAACAGCAGC ACTCGCCCAA GGGACTCAAA 
TCCCTGCCGG AATCGGGGGT GTATCTGCAT GGACAGCATG TGCAGGCCCA GGAGAGCAGA 
CCATCGGTCA TTGAGTCCTC CAACCAGCCC ATGATCATCG AATGCACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261442.1
CDS	626..4276
Translation	MHGHHNHNHLPQQQPQNPQEQQSGAIMKAANQPHHPAHSHPLNHPHPHQHAQPHPHPHPHPHHKLSARRARSRSRSRSPTPDGNQDYDVTRMREEDFERLAVYLVPDVQAERGLPNRADKTLPRSLTLKSSMVYSTPNVKTEGVWSSGVIPRGTRFGPFEGIPTSNYPNDKNKARYFWRVQGTRHITYGNIKIFKDDDYYYLDGSDRSQSNWMRYVASAYSLSVMNLVACQHQENIYFYTTRDILPNEELMVWYCKDFASRLGYDVDPETTIFGACKQAVEAEEEADEEEEEAEGEDGKPRYSMPAPEIPPDVAVSHITYVMGLHLPVGAGNGPANGSVAGSVSGATPPPPTATPCCRRSSPPAHVSTTTSTCNAHHPHIQHGRHASVIIGQDRSPMASSDKDTAGSPLSGLDHQMTPQDGSVRSVRSDEGYHSNECHEDGLTPPEDSSDSESEHNYVLDCSKKAIAPKETVIAQAQKPSSSPPAVVMANTTTNSMISHSSPTATPICETDKNEYRKFKVKMPLKYEFKNKTCVKQEPSLKEVDQEMSLPQEEEEDQVMHPEPDSICPSTTTHLGDEHMLMMERERERERERIQEREPSNQQPASSTVIVLEHNSGGQARTIVPLSKPYYEPDPPGERYMRFGQPSSSILETILTSQHRLEAAAAAANACRQANAATPPPTSPTEMAYSYKKSQRYGNAVSPDSSSNLGQNPEQLSSSAVVVGEQEMTRATMIKGECSPPPPSHHHHNVIFSPSRHAAYLGAGEAGGHSPSPGYPGYPHYGAAATSTFHSPPHSSHSPFDRQSNASSGAGSATNLHLLQTSTQMLNHPLMQPLTPLQRLSPLRISPPSSLSPDGNSCPRSGSPLSPNSLASRGYRSLPYPLKKKDGKMHYECNVCCKTFGQLSNLKVHLRTHSGERPFKCNVCTKSFTQLAHLQKHHLVHTGEKPHQCDICKKRFSSTSNLKTHLRLHSGQKPYACDLCPQKFTQFVHLKLHKRLHTNDRPYVCQGCDKKYISASGLRTHWKTTSCKPNNLEEELAMAAAATSECLDKDHPEPDSREAYEQLTQHMHPAVHPGLRHLSSGGQSPPRLIPLGNHMAPQQQSHQQQQQQHQQQGVPPPHLLMTQHSVGPAPMLLTTASQLPPPPPHHQQNSPSRLLQHGHAHPLQMQQQQQQQQQHSPKGLKSLPESGVYLHGQHVQAQESRPSVIESSNQPMIIECT

Target ORF information:

RefSeq Version	NM_001274513.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Blimp-1, transcript variant B (Blimp-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274513.1

ORF Insert Sequence:

ATGCATGGAC ATCACAACCA CAACCATCTG CCGCAGCAGC AGCCACAAAA TCCGCAGGAG 
CAGCAGTCGG GTGCCATCAT GAAGGCAGCC AACCAGCCCC ACCATCCCGC CCACAGCCAT 
CCGCTCAATC ACCCACATCC GCACCAGCAT GCCCAGCCAC ATCCGCATCC ACATCCCCAT 
CCGCATCACA AGCTGTCGGC GAGAAGGGCG CGCAGTCGCA GTCGAAGTCG CAGTCCGACG 
CCGGATGGCA ATCAGGACTA CGATGTGACC AGGATGCGGG AGGAGGACTT CGAGCGGCTG 
GCCGTCTACC TGGTGCCGGA TGTCCAGGCG GAGCGGGGAC TGCCCAATCG GGCGGACAAG 
ACGCTGCCGC GCAGCCTGAC GCTCAAGTCA TCCATGGTCT ACTCAACGCC AAATGTTAAG 
ACTGAAGGAG TTTGGAGCAG CGGGGTCATC CCACGTGGCA CTCGCTTTGG CCCATTCGAA 
GGCATTCCAA CCTCTAATTA TCCCAATGAT AAGAATAAGG CGCGCTATTT CTGGCGGGTG 
CAGGGAACAC GCCACATAAC CTACGGGAAT ATTAAGATCT TCAAGGACGA TGACTACTAT 
TACTTGGATG GTTCCGATCG CTCGCAGTCC AATTGGATGC GCTATGTGGC CTCCGCTTAT 
AGTTTATCCG TAATGAACCT GGTGGCGTGC CAGCACCAGG AGAACATCTA TTTCTACACC 
ACCCGCGACA TTCTGCCCAA CGAGGAGCTA ATGGTTTGGT ACTGCAAGGA CTTCGCCAGT 
CGCCTGGGCT ACGATGTGGA TCCTGAGACG ACGATCTTCG GCGCATGCAA GCAGGCTGTG 
GAAGCGGAGG AGGAGGCGGA CGAGGAGGAG GAAGAGGCGG AGGGAGAGGA CGGAAAGCCG 
CGATACTCGA TGCCAGCTCC GGAAATTCCA CCCGATGTGG CCGTGAGTCA CATAACCTAC 
GTCATGGGTC TCCATCTGCC TGTGGGAGCG GGAAATGGTC CGGCGAATGG TTCGGTTGCG 
GGTTCCGTTT CCGGTGCCAC ACCTCCACCA CCGACTGCCA CGCCATGCTG CCGCCGCTCC 
AGTCCACCCG CCCACGTCAG CACCACCACC TCCACTTGCA ACGCCCACCA CCCGCACATC 
CAGCATGGTC GACATGCCTC CGTAATCATC GGCCAGGATC GATCGCCTAT GGCCAGCAGT 
GACAAGGATA CGGCGGGATC TCCCCTTTCC GGTCTGGACC ATCAGATGAC CCCGCAGGAT 
GGCAGCGTGA GGTCTGTGAG ATCGGACGAG GGCTACCACA GCAACGAGTG CCACGAGGAT 
GGACTCACAC CACCCGAGGA TTCCAGCGAC AGCGAGAGTG AACATAACTA CGTGCTGGAC 
TGCTCGAAGA AGGCAATTGC TCCCAAAGAG ACAGTGATTG CCCAGGCCCA AAAGCCCAGC 
AGTTCTCCAC CGGCAGTGGT AATGGCCAAC ACGACTACCA ACTCGATGAT TAGCCACAGT 
TCACCCACTG CGACGCCCAT TTGCGAAACG GACAAGAATG AGTATAGAAA GTTCAAGGTG 
AAAATGCCGC TGAAGTACGA GTTCAAGAAC AAGACCTGCG TAAAACAGGA GCCAAGCCTT 
AAGGAAGTGG ACCAGGAGAT GTCCTTGCCG CAGGAGGAGG AGGAGGATCA GGTGATGCAT 
CCGGAACCCG ATTCAATTTG CCCATCGACC ACCACCCATC TGGGCGATGA GCACATGCTG 
ATGATGGAGC GGGAACGGGA AAGGGAGCGC GAAAGGATCC AGGAGAGGGA ACCATCCAAC 
CAGCAGCCCG CCTCCTCCAC GGTCATCGTA CTGGAACACA ACTCTGGCGG TCAAGCCCGC 
ACCATTGTCC CACTGAGTAA ACCCTACTAC GAACCAGATC CTCCCGGCGA ACGCTATATG 
CGATTTGGCC AGCCCTCTTC CAGCATCCTG GAGACTATCC TCACCAGCCA ACATCGCTTG 
GAGGCGGCAG CTGCTGCGGC AAATGCCTGC CGACAGGCCA ATGCCGCCAC ACCACCGCCC 
ACATCGCCCA CGGAGATGGC CTACAGCTAC AAGAAATCCC AGCGGTACGG TAATGCCGTG 
AGTCCGGATA GTAGTTCCAA TCTGGGACAG AATCCCGAGC AGCTTTCCTC GTCGGCAGTG 
GTGGTCGGCG AACAGGAAAT GACCAGAGCC ACCATGATCA AGGGAGAGTG CTCACCACCA 
CCGCCTAGCC ACCATCATCA CAATGTCATC TTCTCGCCCT CGAGACATGC TGCTTATCTG 
GGAGCCGGCG AAGCGGGTGG CCACTCACCG AGTCCGGGTT ATCCTGGCTA TCCCCACTAT 
GGAGCTGCAG CCACCTCGAC CTTCCACTCA CCACCACATA GTTCCCATTC CCCGTTCGAT 
CGCCAGTCGA ATGCATCCAG TGGTGCGGGT TCGGCCACAA ATCTGCATTT ATTGCAAACC 
AGCACCCAAA TGCTGAACCA TCCGCTGATG CAGCCCCTCA CACCCCTGCA GCGTTTGTCC 
CCGCTGAGGA TATCCCCACC CAGCAGCCTC AGTCCTGATG GCAACTCGTG TCCCCGCAGC 
GGCAGCCCCT TGAGTCCCAA TTCCTTGGCC TCCAGAGGCT ACCGATCGTT GCCTTATCCG 
CTCAAGAAGA AGGATGGCAA GATGCACTAC GAGTGCAATG TGTGCTGCAA GACTTTTGGC 
CAATTGAGCA ACCTCAAGGT GCACCTGAGA ACCCATTCCG GCGAAAGGCC CTTCAAGTGC 
AATGTGTGCA CGAAGAGCTT CACCCAGTTG GCGCACCTCC AGAAGCATCA TCTGGTGCAT 
ACGGGCGAGA AACCGCATCA GTGCGACATC TGCAAGAAGC GTTTCTCGAG CACCTCCAAC 
CTGAAGACCC ATTTGAGGCT GCACAGTGGT CAGAAACCAT ATGCCTGTGA TCTCTGCCCG 
CAGAAGTTCA CACAGTTTGT GCATCTGAAG TTGCACAAGA GACTGCACAC GAACGACAGG 
CCCTATGTGT GCCAGGGTTG CGACAAGAAG TACATCAGTG CATCCGGCTT GAGGACCCAC 
TGGAAGACCA CCAGCTGCAA GCCCAACAAT CTGGAGGAGG AGCTGGCCAT GGCAGCAGCG 
GCAACATCGG AGTGTTTGGA TAAAGACCAT CCCGAACCGG ACTCCCGAGA AGCCTACGAG 
CAACTGACGC AGCACATGCA TCCTGCTGTG CATCCGGGAT TGAGGCACCT CTCGAGTGGG 
GGTCAATCGC CACCGCGCCT GATACCCCTG GGCAATCACA TGGCGCCACA ACAGCAGTCG 
CATCAGCAGC AGCAACAGCA GCATCAGCAG CAGGGAGTGC CACCGCCCCA CTTACTGATG 
ACCCAGCATT CGGTGGGTCC GGCACCCATG CTGCTGACCA CCGCCAGCCA GTTGCCACCA 
CCGCCGCCAC ATCATCAGCA GAACTCGCCC AGCCGTCTGC TGCAACATGG CCACGCACAT 
CCGTTGCAGA TGCAACAGCA ACAGCAGCAG CAACAGCAGC ACTCGCCCAA GGGACTCAAA 
TCCCTGCCGG AATCGGGGGT GTATCTGCAT GGACAGCATG TGCAGGCCCA GGAGAGCAGA 
CCATCGGTCA TTGAGTCCTC CAACCAGCCC ATGATCATCG AATGCACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Blimp-1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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