Klp61F cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Klp61F
Entrez Gene ID	38135
Full Name	Kinesin-like protein at 61F
Synonyms	6345,CG9191,CG9191-PA,DmKLP61F,DmKRP130,DmKlp61F,Dmel\CG9191,KIF 8,KIF11,KLP2,KLP61,KLP61F,KLP61f,KRP130,KRP[[(130)]],KRP[[130]],Kin-5,Klp 61F,Klp2,Klp61F/KRP130,Klp61f,Krp130,klp61F,klp61f,l(3)07012,l(3)6D283,l(3)Klp61F,urc,urcn
General protein information	Preferred Names Kinesin-like protein at 61F Names CG9191 gene product from transcript CG9191-RA CG9191-PA Kinesin-related protein-130kD Kinesin8B Klp61F-PA kinesin-5 kinesin-5 motor kinesin-5s kinesin-like protein urchin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 61F4-61F4

mRNA and Protein(s)

mRNA	Protein	Name
NM_057470.5	NP_476818.1	Kinesin-like protein at 61F

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Kif11	NP_034745.1
Pan troglodytes (chimpanzee)	KIF11	XP_507923.2
Xenopus tropicalis (tropical clawed frog)	kif11	NP_001016116.2
Canis lupus familiaris (dog)	KIF11	XP_534964.3
Bos taurus (cattle)	KIF11	NP_001179114.1
Danio rerio (zebrafish)	kif11	NP_775368.1
Rattus norvegicus (Norway rat)	Kif11	NP_001162583.1
Drosophila melanogaster (fruit fly)	Klp61F	NP_476818.1
Homo sapiens (human)	KIF11	NP_004514.2
Gallus gallus (chicken)	KIF11	NP_001026401.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

KLP61F is required for spindle and centromere symmetry in oocytes.
Title: Cooperation Between Kinesin Motors Promotes Spindle Symmetry and Chromosome Organization in Oocytes.

Kinesin-5 pauses at microtubule plus ends and enhances polymerization by stabilizing longitudinal tubulin-tubulin interactions. These activities underlie the ability of kinesin-5 to slide and stabilize microtubule bundles in cells.
Title: Kinesin-5 is a microtubule polymerase.

Klp61F knockdown inhibits post-Golgi transport in Drosophila S2 cells.
Title: Kinesin-5/Eg5 is important for transport of CARTS from the trans-Golgi network to the cell surface.

A staggered antiparallel coiled-coil 'BASS' (bipolar assembly) domain directs the assembly of four kinesin-5 polypeptides into bipolar minifilaments.
Title: The bipolar assembly domain of the mitotic motor kinesin-5.

Tyrosines in the kinesin-5 (KLP61F)head domain are necessary for phosphorylation by Wee1 and for mitotic spindle integrity.
Title: Tyrosines in the kinesin-5 head domain are necessary for phosphorylation by Wee1 and for mitotic spindle integrity.

The following Klp61F gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Klp61F cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa17119	NM_057470.5 Latest version!	Drosophila melanogaster Kinesin-like protein at 61F (Klp61F), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Klp61F ( NM_057470.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476818.1 Drosophila melanogaster Kinesin-like protein at 61F (Klp61F), mRNA.

Price & Availability↑

CloneID	OFa17119
Clone ID Related Accession (Same CDS sequence)	NM_057470.5
Accession Version	NM_057470.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Kinesin-like protein at 61F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_057470.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACATAT CTGGTGGGAA TACGTCGCGC CAGCCCCAAA AGAAGTCCAA CCAAAACATC 
CAGGTGTATG TGCGCGTCAG ACCCCTTAAT TCTCGGGAAC GTTGCATCCG CTCGGCCGAA 
GTCGTGGATG TGGTCGGACC ACGGGAAGTG GTCACCCGCC ACACGCTGGA CTCCAAGCTC 
ACCAAGAAGT TCACCTTTGA CCGCAGTTTT GGCCCCGAGT CCAAGCAGTG CGATGTCTAC 
TCCGTCGTGG TGTCTCCGCT GATCGAGGAG GTCCTCAATG GCTATAACTG CACGGTGTTT 
GCTTATGGCC AGACGGGCAC AGGGAAGACC CACACCATGG TGGGCAACGA GACTGCCGAA 
CTGAAATCCT CCTGGGAAGA TGACTCTGAC ATTGGCATCA TACCGCGCGC TCTGAGTCAC 
CTTTTCGATG AGCTGCGCAT GATGGAGGTG GAGTACACTA TGCGCATTTC CTACTTGGAA 
CTGTACAATG AGGAGCTGTG CGATCTACTG TCCACCGATG ACACCACCAA GATACGCATT 
TTCGATGACA GCACCAAGAA GGGATCGGTG ATTATCCAGG GCCTGGAGGA GATACCAGTG 
CACAGCAAGG ATGATGTGTA CAAGCTGCTG GAGAAGGGAA AGGAGCGTCG CAAAACAGCC 
ACTACGCTGA TGAATGCACA GTCCTCACGC TCCCACACTG TATTTTCTAT AGTTGTGCAC 
ATCAGGGAGA ATGGCATCGA AGGAGAGGAC ATGCTGAAAA TCGGTAAACT GAATCTGGTG 
GATCTGGCGG GCAGTGAAAA TGTTTCCAAG GCTGGGAATG AAAAGGGAAT ACGTGTGCGG 
GAAACAGTAA ACATCAATCA GAGCCTCTTG ACTCTCGGTA GAGTAATTAC CGCTTTGGTC 
GATCGCGCTC CTCACGTTCC ATATCGTGAA TCAAAGCTGA CTAGACTGCT GCAAGAATCT 
CTGGGTGGCA GGACCAAGAC CTCCATCATA GCCACCATAT CGCCGGGCCA CAAGGACATC 
GAGGAGACCC TGAGCACTTT AGAGTACGCT CATCGCGCCA AGAACATTCA AAACAAGCCC 
GAAGTCAATC AGAAGCTGAC CAAAAAGACT GTGCTCAAGG AATACACCGA AGAAATCGAC 
AAGCTTAAAA GAGATCTTAT GGCGGCCAGG GACAAGAATG GCATCTATTT GGCCGAGGAA 
ACCTACGGGG AGATAACTTT GAAGTTAGAA TCCCAGAACC GCGAGCTTAA CGAGAAAATG 
CTGCTGCTCA AGGCTTTGAA GGACGAACTC CAGAACAAGG AGAAGATCTT CAGTGAGGTG 
AGCATGAGTC TTGTGGAGAA AACGCAGGAG CTGAAAAAGA CCGAGGAGAA CCTACTGAAC 
ACGAAGGGTA CTTTGCTCCT GACCAAGAAA GTGCTGACCA AGACCAAGCG ACGATACAAG 
GAAAAAAAGG AGTTGGTGGC CTCGCATATG AAAACGGAGC AGGTTTTGAC CACACAGGCT 
CAGGAGATCC TAGCCGCTGC TGATCTGGCC ACTGATGATA CTCATCAGCT GCACGGAACC 
ATCGAAAGAA GGCGTGAGTT GGATGAGAAG ATTCGCCGAT CTTGCGATCA GTTCAAAGAT 
CGTATGCAGG ATAATTTGGA AATGATCGGT GGCAGCCTGA ACTTGTATCA GGATCAGCAG 
GCAGCCCTCA AGGAGCAGCT AAGCCAGGAA ATGGTAAACT CGAGCTATGT GAGCCAACGC 
CTTGCACTAA ATTCCAGCAA AAGCATCGAA ATGCTTAAGG AGATGTGTGC CCAATCGCTA 
CAGGATCAGA CCAACCTCCA TAATAAGTTA ATAGGAGAAG TCATGAAGAT CAGCGATCAG 
CACTCTCAGG CATTTGTCGC CAAGCTGATG GAGCAGATGC AGCAGCAACA GCTTTTGATG 
AGCAAAGAAA TTCAGACTAA CCTGCAGGTG ATCGAGGAAA ACAACCAGCG GCACAAAGCC 
ATGCTGGACT CTATGCAGGA AAAGTTCGCC ACCATAATTG ACAGCAGCTT ACAGTCTGTG 
GAAGAACATG CGAAGCAAAT GCACAAGAAG TTGGAGCAGC TCGGGGCAAT GAGTTTGCCA 
GATGCGGAGG AACTGCAGAA TCTTCAAGAG GAGTTGGCGA ATGAACGAGC TCTGGCGCAG 
CAGGAGGATG CTCTCCTTGA GTCCATGATG ATGCAGATGG AACAGATCAA GAACTTGCGG 
TCCAAAAATA GCATCAGCAT GTCCGTACAT CTTAATAAGA TGGAGGAGAG TCGACTGACG 
AGAAATCATC GCATCGATGA TATTAAGTCT GGTATCCAAG ACTACCAAAA GTTGGGCATA 
GAAGCTTCCC AATCCGCACA AGCGGAGCTC ACCAGCCAAA TGGAGGCGGG AATGCTTTGT 
CTGGACCAAG GCGTTGCCAA CTGTTCGATG CTTCAGGTGC ACATGAAGAA TCTCAACCAG 
AAATACGAAA AGGAAACAAA TGAGAATGTT GGTTCCGTTC GAGTGCACCA CAATCAGGTG 
GAAATTATTT GCCAAGAGAG CAAGCAGCAG CTTGAGGCGG TGCAGGAGAA AACCGAAGTT 
AACTTGGAAC AGATGGTGGA TGCAAGGCAG CAGCTTATCA CCGAGGACAG ACAGCGATTC 
ATAGGCCATG CCACTGTAGC CACTGATCTT GTCCAAGAGT CGAACCGGCA GTTCTCGGAG 
CATGCTGAAC ACCAACGGCA GCAGCTGCAA ATTTGCGAGC AAGAGCTTGT ACGCTTCCAA 
CAGTCGGAGT TGAAAACGTA CGCCCCAACT GGGACCACGC CCTCCAAAAG GGATTTCGTT 
TATCCGCGCA CCCTCGTGGC CACGTCGCCA CATCAGGAAA TTGTGAGGCG CTATCGCCAA 
GAGCTGGACT GGTCAGATCT GGACACCACG GCCACTATAG ATGAGTGCAG CGAGGGTGAG 
CATGACGTCT CCATGCATTC CGTCCAGGAG CTGTCCGAAA CTGAAACCAT AATGAACTCC 
ACGCCCATTG AGCCCGTGGA TGGTGTCACC GTGAAGCGTG GGTGCGGCAC CACCCGCAAT 
TCCAATTCGA ACGCCCTAAA GCCACCCGTA GCTACTGGTG GAAAACGCAG CAGCTCGTTG 
TCACGTTCCC TGACACCCAG TAAAACGTCA CCTCGAGGTT CTCCCGCCTT CGTCAGGCAC 
AACAAAGAAA ACGTAGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476818.1
CDS	271..3471
Translation	MDISGGNTSRQPQKKSNQNIQVYVRVRPLNSRERCIRSAEVVDVVGPREVVTRHTLDSKLTKKFTFDRSFGPESKQCDVYSVVVSPLIEEVLNGYNCTVFAYGQTGTGKTHTMVGNETAELKSSWEDDSDIGIIPRALSHLFDELRMMEVEYTMRISYLELYNEELCDLLSTDDTTKIRIFDDSTKKGSVIIQGLEEIPVHSKDDVYKLLEKGKERRKTATTLMNAQSSRSHTVFSIVVHIRENGIEGEDMLKIGKLNLVDLAGSENVSKAGNEKGIRVRETVNINQSLLTLGRVITALVDRAPHVPYRESKLTRLLQESLGGRTKTSIIATISPGHKDIEETLSTLEYAHRAKNIQNKPEVNQKLTKKTVLKEYTEEIDKLKRDLMAARDKNGIYLAEETYGEITLKLESQNRELNEKMLLLKALKDELQNKEKIFSEVSMSLVEKTQELKKTEENLLNTKGTLLLTKKVLTKTKRRYKEKKELVASHMKTEQVLTTQAQEILAAADLATDDTHQLHGTIERRRELDEKIRRSCDQFKDRMQDNLEMIGGSLNLYQDQQAALKEQLSQEMVNSSYVSQRLALNSSKSIEMLKEMCAQSLQDQTNLHNKLIGEVMKISDQHSQAFVAKLMEQMQQQQLLMSKEIQTNLQVIEENNQRHKAMLDSMQEKFATIIDSSLQSVEEHAKQMHKKLEQLGAMSLPDAEELQNLQEELANERALAQQEDALLESMMMQMEQIKNLRSKNSISMSVHLNKMEESRLTRNHRIDDIKSGIQDYQKLGIEASQSAQAELTSQMEAGMLCLDQGVANCSMLQVHMKNLNQKYEKETNENVGSVRVHHNQVEIICQESKQQLEAVQEKTEVNLEQMVDARQQLITEDRQRFIGHATVATDLVQESNRQFSEHAEHQRQQLQICEQELVRFQQSELKTYAPTGTTPSKRDFVYPRTLVATSPHQEIVRRYRQELDWSDLDTTATIDECSEGEHDVSMHSVQELSETETIMNSTPIEPVDGVTVKRGCGTTRNSNSNALKPPVATGGKRSSSLSRSLTPSKTSPRGSPAFVRHNKENVA

Target ORF information:

RefSeq Version	NM_057470.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Kinesin-like protein at 61F (Klp61F), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057470.5

ORF Insert Sequence:

ATGGACATAT CTGGTGGGAA TACGTCGCGC CAGCCCCAAA AGAAGTCCAA CCAAAACATC 
CAGGTGTATG TGCGCGTCAG ACCCCTTAAT TCTCGGGAAC GTTGCATCCG CTCGGCCGAA 
GTCGTGGATG TGGTCGGACC ACGGGAAGTG GTCACCCGCC ACACGCTGGA CTCCAAGCTC 
ACCAAGAAGT TCACCTTTGA CCGCAGTTTT GGCCCCGAGT CCAAGCAGTG CGATGTCTAC 
TCCGTCGTGG TGTCTCCGCT GATCGAGGAG GTCCTCAATG GCTATAACTG CACGGTGTTT 
GCTTATGGCC AGACGGGCAC AGGGAAGACC CACACCATGG TGGGCAACGA GACTGCCGAA 
CTGAAATCCT CCTGGGAAGA TGACTCTGAC ATTGGCATCA TACCGCGCGC TCTGAGTCAC 
CTTTTCGATG AGCTGCGCAT GATGGAGGTG GAGTACACTA TGCGCATTTC CTACTTGGAA 
CTGTACAATG AGGAGCTGTG CGATCTACTG TCCACCGATG ACACCACCAA GATACGCATT 
TTCGATGACA GCACCAAGAA GGGATCGGTG ATTATCCAGG GCCTGGAGGA GATACCAGTG 
CACAGCAAGG ATGATGTGTA CAAGCTGCTG GAGAAGGGAA AGGAGCGTCG CAAAACAGCC 
ACTACGCTGA TGAATGCACA GTCCTCACGC TCCCACACTG TATTTTCTAT AGTTGTGCAC 
ATCAGGGAGA ATGGCATCGA AGGAGAGGAC ATGCTGAAAA TCGGTAAACT GAATCTGGTG 
GATCTGGCGG GCAGTGAAAA TGTTTCCAAG GCTGGGAATG AAAAGGGAAT ACGTGTGCGG 
GAAACAGTAA ACATCAATCA GAGCCTCTTG ACTCTCGGTA GAGTAATTAC CGCTTTGGTC 
GATCGCGCTC CTCACGTTCC ATATCGTGAA TCAAAGCTGA CTAGACTGCT GCAAGAATCT 
CTGGGTGGCA GGACCAAGAC CTCCATCATA GCCACCATAT CGCCGGGCCA CAAGGACATC 
GAGGAGACCC TGAGCACTTT AGAGTACGCT CATCGCGCCA AGAACATTCA AAACAAGCCC 
GAAGTCAATC AGAAGCTGAC CAAAAAGACT GTGCTCAAGG AATACACCGA AGAAATCGAC 
AAGCTTAAAA GAGATCTTAT GGCGGCCAGG GACAAGAATG GCATCTATTT GGCCGAGGAA 
ACCTACGGGG AGATAACTTT GAAGTTAGAA TCCCAGAACC GCGAGCTTAA CGAGAAAATG 
CTGCTGCTCA AGGCTTTGAA GGACGAACTC CAGAACAAGG AGAAGATCTT CAGTGAGGTG 
AGCATGAGTC TTGTGGAGAA AACGCAGGAG CTGAAAAAGA CCGAGGAGAA CCTACTGAAC 
ACGAAGGGTA CTTTGCTCCT GACCAAGAAA GTGCTGACCA AGACCAAGCG ACGATACAAG 
GAAAAAAAGG AGTTGGTGGC CTCGCATATG AAAACGGAGC AGGTTTTGAC CACACAGGCT 
CAGGAGATCC TAGCCGCTGC TGATCTGGCC ACTGATGATA CTCATCAGCT GCACGGAACC 
ATCGAAAGAA GGCGTGAGTT GGATGAGAAG ATTCGCCGAT CTTGCGATCA GTTCAAAGAT 
CGTATGCAGG ATAATTTGGA AATGATCGGT GGCAGCCTGA ACTTGTATCA GGATCAGCAG 
GCAGCCCTCA AGGAGCAGCT AAGCCAGGAA ATGGTAAACT CGAGCTATGT GAGCCAACGC 
CTTGCACTAA ATTCCAGCAA AAGCATCGAA ATGCTTAAGG AGATGTGTGC CCAATCGCTA 
CAGGATCAGA CCAACCTCCA TAATAAGTTA ATAGGAGAAG TCATGAAGAT CAGCGATCAG 
CACTCTCAGG CATTTGTCGC CAAGCTGATG GAGCAGATGC AGCAGCAACA GCTTTTGATG 
AGCAAAGAAA TTCAGACTAA CCTGCAGGTG ATCGAGGAAA ACAACCAGCG GCACAAAGCC 
ATGCTGGACT CTATGCAGGA AAAGTTCGCC ACCATAATTG ACAGCAGCTT ACAGTCTGTG 
GAAGAACATG CGAAGCAAAT GCACAAGAAG TTGGAGCAGC TCGGGGCAAT GAGTTTGCCA 
GATGCGGAGG AACTGCAGAA TCTTCAAGAG GAGTTGGCGA ATGAACGAGC TCTGGCGCAG 
CAGGAGGATG CTCTCCTTGA GTCCATGATG ATGCAGATGG AACAGATCAA GAACTTGCGG 
TCCAAAAATA GCATCAGCAT GTCCGTACAT CTTAATAAGA TGGAGGAGAG TCGACTGACG 
AGAAATCATC GCATCGATGA TATTAAGTCT GGTATCCAAG ACTACCAAAA GTTGGGCATA 
GAAGCTTCCC AATCCGCACA AGCGGAGCTC ACCAGCCAAA TGGAGGCGGG AATGCTTTGT 
CTGGACCAAG GCGTTGCCAA CTGTTCGATG CTTCAGGTGC ACATGAAGAA TCTCAACCAG 
AAATACGAAA AGGAAACAAA TGAGAATGTT GGTTCCGTTC GAGTGCACCA CAATCAGGTG 
GAAATTATTT GCCAAGAGAG CAAGCAGCAG CTTGAGGCGG TGCAGGAGAA AACCGAAGTT 
AACTTGGAAC AGATGGTGGA TGCAAGGCAG CAGCTTATCA CCGAGGACAG ACAGCGATTC 
ATAGGCCATG CCACTGTAGC CACTGATCTT GTCCAAGAGT CGAACCGGCA GTTCTCGGAG 
CATGCTGAAC ACCAACGGCA GCAGCTGCAA ATTTGCGAGC AAGAGCTTGT ACGCTTCCAA 
CAGTCGGAGT TGAAAACGTA CGCCCCAACT GGGACCACGC CCTCCAAAAG GGATTTCGTT 
TATCCGCGCA CCCTCGTGGC CACGTCGCCA CATCAGGAAA TTGTGAGGCG CTATCGCCAA 
GAGCTGGACT GGTCAGATCT GGACACCACG GCCACTATAG ATGAGTGCAG CGAGGGTGAG 
CATGACGTCT CCATGCATTC CGTCCAGGAG CTGTCCGAAA CTGAAACCAT AATGAACTCC 
ACGCCCATTG AGCCCGTGGA TGGTGTCACC GTGAAGCGTG GGTGCGGCAC CACCCGCAAT 
TCCAATTCGA ACGCCCTAAA GCCACCCGTA GCTACTGGTG GAAAACGCAG CAGCTCGTTG 
TCACGTTCCC TGACACCCAG TAAAACGTCA CCTCGAGGTT CTCCCGCCTT CGTCAGGCAC 
AACAAAGAAA ACGTAGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Klp61F cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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