ebd1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ebd1
Entrez Gene ID	38082
Full Name	earthbound 1
Synonyms	CG3371,Dmel\CG3371,EP3592,Ebd1
General protein information	Preferred Names earthbound 1 Names CG3371 gene product from transcript CG3371-RA CG3371-PA earthbound ebd1-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 61C8-61C8

mRNA and Protein(s)

mRNA	Protein	Name
NM_138206.2	NP_612050.1	earthbound 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

in the adult intestine, Ebd acts independently of Ewg in physiological Wnt signaling, but cooperates with Ewg to induce the hyperactivation of Wnt target gene expression following Apc1 loss. These findings have relevance for human tumorigenesis, as Jerky (JRK/JH8), the human Ebd homolog, promotes Wnt pathway hyperactivation and is overexpressed in colorectal, breast, and ovarian cancers
Title: Intestinal stem cell overproliferation resulting from inactivation of the APC tumor suppressor requires the transcription cofactors Earthbound and Erect wing.

The following ebd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ebd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa20374	NM_138206.2 Latest version!	Drosophila melanogaster earthbound 1 (ebd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ebd1 ( NM_138206.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_612050.1 Drosophila melanogaster earthbound 1 (ebd1), mRNA.

Price & Availability↑

CloneID	OFa20374
Clone ID Related Accession (Same CDS sequence)	NM_138206.2
Accession Version	NM_138206.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	earthbound 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_138206.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGGATAC GCGAAAAACG CAAGTTGCAG AGGGAGCTGA GGGTGGAGAA GCGCGTGGAG 
AGGGAGAAAA AGGAGCACAA GGAGGCCTCC AAGACGCAGG ACAACAACAA CAAGGCGAAG 
GCCACTGACA CGGAGGTTCT CACACCAGCT CCTGCGCCTG CGCCTGCTCC ACCAGCAGCA 
CCCACTTCGA CTGCATCACC CACACCCACT AGCACGCACA CCCCCAGCCT GCCGGCCGCC 
GAGAAGGTGG CCTTCGACAA TCGCATCAAG CGCAAGAATG TCCTGGCCCT CAACGAAAAG 
GTTGCTGCTC TGCGGGAATA CGATCGGCTG CCGGTTTATA AGCACGTTGG CCGGCTGTTC 
AACTGCAGCC CAGATCAGAT CAAGCGGATC GTGCAGCAGC GCGCTGAGAT TCTGAACGCG 
TGGGATCAGC GAACCCGCAG GAGCCAGGAC GCCAAGACCA TGGAGACGAA GACCGTCAGG 
GTCTCGATGT TGGGCAAGGC CGTGTACGAT TGGATACGCC GCATGATGTA CTACAAGGAC 
TTCAGCATCT CCGACGGACT CATCCAGAAG ATGGCGCTGC AGTTCAAGAG CTCCATGAAC 
CTGGCACATT TCTTTCCGCA CCAGGAGTGG TGCGACAAGT TCAGACGCAC CTACAGCATC 
CAACACAGCG ACACCAGACT GTTGAAGATA GGCTACACGC AGGGCTACTC GGTGCAGATC 
AAGGACATCG TCAAGGACGT CATGTCGGAA AGCATGCCGG AAAGTGGGCC ACGGAACGGC 
GAGGAGGATG ACGAAGAGGT CAGCTTGGGC AGCCCGGTGA GCAGCCATCC CCGCAGCTAT 
GTAGAAGACG AGGATGACGA GGTGGATGTC GACGGTGGCG GTATTAGCGG CGAGGATGAT 
CTGGAGGAGG AGCCTGATGT CAAGCCCTCA CTAAGCGAAC TAAATTTTGC CAGGGCTCTG 
AAGCCCTTGC CGCCTCTAGT TAGACTGCCC CTCCAAGCTA ACACACCGCC AGGCACCACT 
CAAAAAGTTC TCCTGGCCAC ACCCATTGTT CCGACGCAAG CGGGCGGACA GCCCATGACC 
ATGACCATCA TACCACTAGC CACGCTCGCT CAGAGCATCA CCAAGATTCC TGCCCCACAG 
CAGCAGGACA AAGGACCGGT GGTACCGCCG CCCAAACTAG AGATCAAGCA GGAAAAGGAA 
ATCAAAGTGG AGCCCAAGGA CCCGGAAGAG CTCCTGGCGA AGCAGCCCGA GGCGGAGCTC 
CAGGAGAACG ATGACCGAGC ACAAAGGGAA AAGGATCTTG AAATTCCGCC TACAGTGCAC 
ATAAAAGTGG AGCCAGAGGC TGAGCTGGAA TCGGAACTGG ACCAAGACCC AGCGGATCTC 
GATGAAGACG CGAACAATGG TCGCTCCAGT GTCACCTCGC TGGCCGAGGT TCTGGCGGCC 
AATGTGGAGG ACGAGATGGA GTACATCGAG CAGATGCGGC AGCGAAAGGT GAGTTCGCCT 
GCGGCAGTTG AAAACTCGCT GAATCCTCGC CAGGGCACCA AACGTCGCTA TGAGTCCGCC 
CAAGACGATA ACAACAATGG GGGATCCGGC CAAGAAAGTG GGTCCAGTGT CATCAGCTGC 
GCCGATGCAC GCAAGTACCT AAAGCTACTC GAGGAGTTTG CTCTGTACAA GGAGAACTAC 
CGGCTGATTG AGCTGATCAC ACGAGCCGAT GAAGTGATGC GCGAAATGGA TGACAATGTG 
CCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_612050.1
CDS	403..2148
Translation	MRIREKRKLQRELRVEKRVEREKKEHKEASKTQDNNNKAKATDTEVLTPAPAPAPAPPAAPTSTASPTPTSTHTPSLPAAEKVAFDNRIKRKNVLALNEKVAALREYDRLPVYKHVGRLFNCSPDQIKRIVQQRAEILNAWDQRTRRSQDAKTMETKTVRVSMLGKAVYDWIRRMMYYKDFSISDGLIQKMALQFKSSMNLAHFFPHQEWCDKFRRTYSIQHSDTRLLKIGYTQGYSVQIKDIVKDVMSESMPESGPRNGEEDDEEVSLGSPVSSHPRSYVEDEDDEVDVDGGGISGEDDLEEEPDVKPSLSELNFARALKPLPPLVRLPLQANTPPGTTQKVLLATPIVPTQAGGQPMTMTIIPLATLAQSITKIPAPQQQDKGPVVPPPKLEIKQEKEIKVEPKDPEELLAKQPEAELQENDDRAQREKDLEIPPTVHIKVEPEAELESELDQDPADLDEDANNGRSSVTSLAEVLAANVEDEMEYIEQMRQRKVSSPAAVENSLNPRQGTKRRYESAQDDNNNGGSGQESGSSVISCADARKYLKLLEEFALYKENYRLIELITRADEVMREMDDNVP

Target ORF information:

RefSeq Version	NM_138206.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster earthbound 1 (ebd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138206.2

ORF Insert Sequence:

ATGCGGATAC GCGAAAAACG CAAGTTGCAG AGGGAGCTGA GGGTGGAGAA GCGCGTGGAG 
AGGGAGAAAA AGGAGCACAA GGAGGCCTCC AAGACGCAGG ACAACAACAA CAAGGCGAAG 
GCCACTGACA CGGAGGTTCT CACACCAGCT CCTGCGCCTG CGCCTGCTCC ACCAGCAGCA 
CCCACTTCGA CTGCATCACC CACACCCACT AGCACGCACA CCCCCAGCCT GCCGGCCGCC 
GAGAAGGTGG CCTTCGACAA TCGCATCAAG CGCAAGAATG TCCTGGCCCT CAACGAAAAG 
GTTGCTGCTC TGCGGGAATA CGATCGGCTG CCGGTTTATA AGCACGTTGG CCGGCTGTTC 
AACTGCAGCC CAGATCAGAT CAAGCGGATC GTGCAGCAGC GCGCTGAGAT TCTGAACGCG 
TGGGATCAGC GAACCCGCAG GAGCCAGGAC GCCAAGACCA TGGAGACGAA GACCGTCAGG 
GTCTCGATGT TGGGCAAGGC CGTGTACGAT TGGATACGCC GCATGATGTA CTACAAGGAC 
TTCAGCATCT CCGACGGACT CATCCAGAAG ATGGCGCTGC AGTTCAAGAG CTCCATGAAC 
CTGGCACATT TCTTTCCGCA CCAGGAGTGG TGCGACAAGT TCAGACGCAC CTACAGCATC 
CAACACAGCG ACACCAGACT GTTGAAGATA GGCTACACGC AGGGCTACTC GGTGCAGATC 
AAGGACATCG TCAAGGACGT CATGTCGGAA AGCATGCCGG AAAGTGGGCC ACGGAACGGC 
GAGGAGGATG ACGAAGAGGT CAGCTTGGGC AGCCCGGTGA GCAGCCATCC CCGCAGCTAT 
GTAGAAGACG AGGATGACGA GGTGGATGTC GACGGTGGCG GTATTAGCGG CGAGGATGAT 
CTGGAGGAGG AGCCTGATGT CAAGCCCTCA CTAAGCGAAC TAAATTTTGC CAGGGCTCTG 
AAGCCCTTGC CGCCTCTAGT TAGACTGCCC CTCCAAGCTA ACACACCGCC AGGCACCACT 
CAAAAAGTTC TCCTGGCCAC ACCCATTGTT CCGACGCAAG CGGGCGGACA GCCCATGACC 
ATGACCATCA TACCACTAGC CACGCTCGCT CAGAGCATCA CCAAGATTCC TGCCCCACAG 
CAGCAGGACA AAGGACCGGT GGTACCGCCG CCCAAACTAG AGATCAAGCA GGAAAAGGAA 
ATCAAAGTGG AGCCCAAGGA CCCGGAAGAG CTCCTGGCGA AGCAGCCCGA GGCGGAGCTC 
CAGGAGAACG ATGACCGAGC ACAAAGGGAA AAGGATCTTG AAATTCCGCC TACAGTGCAC 
ATAAAAGTGG AGCCAGAGGC TGAGCTGGAA TCGGAACTGG ACCAAGACCC AGCGGATCTC 
GATGAAGACG CGAACAATGG TCGCTCCAGT GTCACCTCGC TGGCCGAGGT TCTGGCGGCC 
AATGTGGAGG ACGAGATGGA GTACATCGAG CAGATGCGGC AGCGAAAGGT GAGTTCGCCT 
GCGGCAGTTG AAAACTCGCT GAATCCTCGC CAGGGCACCA AACGTCGCTA TGAGTCCGCC 
CAAGACGATA ACAACAATGG GGGATCCGGC CAAGAAAGTG GGTCCAGTGT CATCAGCTGC 
GCCGATGCAC GCAAGTACCT AAAGCTACTC GAGGAGTTTG CTCTGTACAA GGAGAACTAC 
CGGCTGATTG AGCTGATCAC ACGAGCCGAT GAAGTGATGC GCGAAATGGA TGACAATGTG 
CCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

ebd1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報