CG12851 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG12851
Entrez Gene ID	37993
Full Name	CG12851 gene product from transcript CG12851-RD
Synonyms	Dmel\CG12851
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 60E8-60E10

mRNA and Protein(s)

mRNA	Protein	Name
NM_138143.4	NP_611987.3	uncharacterized protein, isoform B
NM_001299936.1	NP_001286865.1	uncharacterized protein, isoform C
NM_001299937.1	NP_001286866.1	uncharacterized protein, isoform D

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG12851 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG12851 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa11269	NM_001299937.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant D (CG12851), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa11269	NM_138143.4 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG12851), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa21959	NM_001299936.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant C (CG12851), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG12851 ( NM_138143.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611987.3 Drosophila melanogaster uncharacterized protein, transcript variant B (CG12851), mRNA.

Price & Availability↑

CloneID	OFa11269
Clone ID Related Accession (Same CDS sequence)	NM_138143.4 , NM_001299937.1
Accession Version	NM_138143.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_138143.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTGGAT CCAAGCTGCG CTCCTGGATG GAGACGCATA TTGTGCGTCC GCGGAAGAAG 
GGCAGTAAAC ACAAGCAAGG GACCGTGGAC GCAGGCAGTT CTTGCGTGGG GAAGAAGGCG 
GGCACCTTAC CAACACAGGG CAGCAACCTA ACTAGCCCGG TCCTGACCAG TAGCGGAAGC 
CACAGCATCG CCAGTCCGGT GCGGAGGCGC GAGGTCTCCC CCATCCAGTG TCACCCACCC 
AGTCGTCGAT ATGGCTGGCA ATCCCCAGAT GAAGATTCCT ACGTCATAGC CTCGCCCAAG 
TCGGACAATT TGCTTCACGC TCCGCACTGC TATCAGCCGC ATCAACCGCT CCAGCAGCCG 
TCGCACCACT TAAGCGTGCC CAATAGCAAG GAAACCTATG CGGAGAAATC GCCCACCAGG 
GTAAACTGCT CTTCGTCCTC CATATCGTCG TTGTCCTGGT CAACGGGATC CAAGGAGCCC 
TCTTCTGGCA AACAAGCAAT TTTTAGAGAG CAGTCCTTAA ATGGGGAATA CCACATCCCA 
CCTGAGGAGG AGGTGGAGTA CGAGGAAGTT GGCGCTTTGC TGCTTCGGCA TGCACCACCC 
ATTGCAGAAC AACAGCGCCC GCAGTCAGAA AACTTAAGTG CCGTGCGTCT AATCTACAAG 
GAAGAGCCAA GGCAAAGCAA GAATCATGTT CGTTACGGTC GCCTGCTTCC CTCCAAGCTA 
GCACCGGCTC AGCATGGCGA GTCACTGGAT AATTTGACAG CTACACCGCC TATTCCACCC 
GCTTCACTTA CCGGCTCGCG TATTCGAACT CCACGAGGAA TTCAGCGCAA TAATTTTATT 
TCGGGACCCC GGCCACACAG CTTGCCCTCG CCAGAAAGCG CTTATTCCAC GGGCTACTCC 
ACAGATGGCA CATCGCCATG CGCCGCCAGC AACTACAATC CACCTGAGTA CTACATTAAC 
ATGCGCTCGG GAACCCACTA TTTCCCGAAG AGCGTCAACT CGCTGGCCGT CGAGGCCCAG 
CGGTACAAGT TCGGCCTTAA CCGCATAGAG GAGATGTCGC CTATGGATCC GTTGCCCAAG 
AGCAACTTCG CAACTGCCAC TCATGGCCCG GAGTCGTCGC CTAGTTCGCT AGCTCTGCGG 
CAGCAGTCAA AGCTCTTTGA GTCTCCCTCG CCGAGACAGC GCTGTCGTAT AAGAACAAAT 
CCCTGGTACA ACACGGGAGA GCTCTTGCCT AGCACGACAG GCCCCACACG CTTGGATCTG 
GATGCGACCA GTACTACCTC CACGACCTCG TCGGGCATTA AGTCAAGCAA TGAACTGGAA 
GTTCCGGTGG CCAAAACAAT AGCAAACAAA GCAGGGAAGC CAGTCCCTTA TGCAGCAACC 
TCCAACTGCA GAGCTACTCC TAGCCAGGCA GCCAAACAGG CTGATATAGA CATTGTTGGG 
GGAGGGGCTG GTGTTGCACC TGGGGCTGGA GTTGTTTATG AATCCGAGGG CTCCTCGACT 
TCGACTGAAT TGGAAAATGT GCATGCCCCA CATACCATTA AGCGGCGTCA CTTGGACCAC 
GCCGAGACTA CAACAACTTC AGCGCCCGTG GCAGCTGGTG GTGGAACTGG AGCTACCACT 
ACATCCTTCG TTCTTAGTGA TGACGAGGCC ACCCTTAATG AAATGATTGG GAAGTTCGAC 
GAGAGTTACA TTTACGAAAA GGAAACTGAT ATATTAAGCA GTGATTCAGA CCTTACGGAC 
TGCTGTGCTT CGGAGCTAGA TACTGGACAG GATGCGGGTG ATGAGTGTGA CACAGACGAG 
TTGTTGGACA TTGACTTTAT AGACACTTCA TCCATGCAAG AGGTGGAACT TGACCGCAAG 
GATCTGTCAC GAAATCTGGG CAACTGTCAC TACTATTCTA ATCACCCTAT CAGTCCGGTG 
CTCAATCGAA AGGCATCAGT CCGTTCCCGA CGCAAGAGCG GTCAGGAGAC GGGGGAAAGC 
TCCCAACAGC GCCGACGCAA GAGGTTCCTT AAAACACGTA AGAAGAGTTG TGAAAATCGC 
GAAAAGACTT CTTCGTCACC TCTTCGTGAG CCTCGTGGCA CCCGAAGTGT GGGTGGCACG 
CCCGTCTGTT TGCGCCGCAA TCTACTGGGT CCAAGTGAGA GAATATACAA AACGTCTCCC 
CTGTCAAACC GCTCCAACTC GCTAATCTTT GGAAGCATAA AAAAAAAGTC AACACTCACC 
ATCGCAGAAA GCGAAAAAGC ACTTCTTAAG GCAGACTTGG AGGCTGATAT GAAATATAAA 
CAACTAATTA TGGAAGCGGA GTCTTTGCTG GAATCGATGA AGAACAGCTT ACAGAGCATT 
CCCAGAGACA CGCCTGTCGC CAGCCCAAGA CGAGTGAATC CCTTAGCTAA CAAGCGGGTG 
GAAATGCTTA AAAACAGTGA AGTGGTGGAC TCCAAAAAAC AGACTCTGGT TCTTAACTCT 
CCTCCTCTTG AGCATGAGTT AGCAGCAGCC ATTAATAAGC GCATAGAGAT GCTCAAGTTT 
GAGACATCGT CTGCTTCTTC GCCACCAAAC AGTCCCAAGC CTGCCCGTTA CAGTCCTCGC 
AAAACGCATC TGACCAACTT TATGAATCAA AATGCTCCAC CAGAGTTGCC ACCACGAAAA 
ATAAAAGGCA ACCCTCCCTC TGCTCCACTC TCGCCACAAC TTCAGCTAAA TGGAAGGAGT 
ATTCAGGAGA GTCCAAAGAG CAAGCGACGC ACGATGATGG TGACTTCTTC TACCACGACA 
ACGGTGATCA GCTTCAACGG CAGTGATCCT GAGATGGAGT GCATAGCTGT AGAGGATATT 
GGCAATCACA ACTACTTGCC TCAGCCGCAA TCGAATACGA GGGATGGAAA ATATGGATCG 
AATACGAAAA TGGAAAACCC ATTGCATGCC GAATTAAATA TTAATAACAA CTTTTACTGC 
CCACACAGCG AGCCCTTAAA GCGCAAGGTA TACAAGGGTA GCTCATCGTT CGAGCGCATA 
AAAAAAAACT TCGACTTCGA ATTGGATTCA AACGGACGGT GCAAGTCAAA TGAGCGTTCG 
CAAATTAAGC TTTTGGCCTC CGTGTCGGCC AAGAGCTCTA TGCAAGACGT GACCGTTGAC 
GAGGCCAAGG GCCATGCAAT AGGCGGTAGT AACCGCAAGC AGCAGCTCAT ACTGAATACC 
ATTTTCGATT TAAAGCGCAG CTTGGAGCAT CAAAGCCATC AACTGAGTGG ATTTAATGGC 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611987.3
CDS	801..4046
Translation	MFGSKLRSWMETHIVRPRKKGSKHKQGTVDAGSSCVGKKAGTLPTQGSNLTSPVLTSSGSHSIASPVRRREVSPIQCHPPSRRYGWQSPDEDSYVIASPKSDNLLHAPHCYQPHQPLQQPSHHLSVPNSKETYAEKSPTRVNCSSSSISSLSWSTGSKEPSSGKQAIFREQSLNGEYHIPPEEEVEYEEVGALLLRHAPPIAEQQRPQSENLSAVRLIYKEEPRQSKNHVRYGRLLPSKLAPAQHGESLDNLTATPPIPPASLTGSRIRTPRGIQRNNFISGPRPHSLPSPESAYSTGYSTDGTSPCAASNYNPPEYYINMRSGTHYFPKSVNSLAVEAQRYKFGLNRIEEMSPMDPLPKSNFATATHGPESSPSSLALRQQSKLFESPSPRQRCRIRTNPWYNTGELLPSTTGPTRLDLDATSTTSTTSSGIKSSNELEVPVAKTIANKAGKPVPYAATSNCRATPSQAAKQADIDIVGGGAGVAPGAGVVYESEGSSTSTELENVHAPHTIKRRHLDHAETTTTSAPVAAGGGTGATTTSFVLSDDEATLNEMIGKFDESYIYEKETDILSSDSDLTDCCASELDTGQDAGDECDTDELLDIDFIDTSSMQEVELDRKDLSRNLGNCHYYSNHPISPVLNRKASVRSRRKSGQETGESSQQRRRKRFLKTRKKSCENREKTSSSPLREPRGTRSVGGTPVCLRRNLLGPSERIYKTSPLSNRSNSLIFGSIKKKSTLTIAESEKALLKADLEADMKYKQLIMEAESLLESMKNSLQSIPRDTPVASPRRVNPLANKRVEMLKNSEVVDSKKQTLVLNSPPLEHELAAAINKRIEMLKFETSSASSPPNSPKPARYSPRKTHLTNFMNQNAPPELPPRKIKGNPPSAPLSPQLQLNGRSIQESPKSKRRTMMVTSSTTTTVISFNGSDPEMECIAVEDIGNHNYLPQPQSNTRDGKYGSNTKMENPLHAELNINNNFYCPHSEPLKRKVYKGSSSFERIKKNFDFELDSNGRCKSNERSQIKLLASVSAKSSMQDVTVDEAKGHAIGGSNRKQQLILNTIFDLKRSLEHQSHQLSGFNGE

Target ORF information:

RefSeq Version	NM_138143.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG12851), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138143.4

ORF Insert Sequence:

ATGTTTGGAT CCAAGCTGCG CTCCTGGATG GAGACGCATA TTGTGCGTCC GCGGAAGAAG 
GGCAGTAAAC ACAAGCAAGG GACCGTGGAC GCAGGCAGTT CTTGCGTGGG GAAGAAGGCG 
GGCACCTTAC CAACACAGGG CAGCAACCTA ACTAGCCCGG TCCTGACCAG TAGCGGAAGC 
CACAGCATCG CCAGTCCGGT GCGGAGGCGC GAGGTCTCCC CCATCCAGTG TCACCCACCC 
AGTCGTCGAT ATGGCTGGCA ATCCCCAGAT GAAGATTCCT ACGTCATAGC CTCGCCCAAG 
TCGGACAATT TGCTTCACGC TCCGCACTGC TATCAGCCGC ATCAACCGCT CCAGCAGCCG 
TCGCACCACT TAAGCGTGCC CAATAGCAAG GAAACCTATG CGGAGAAATC GCCCACCAGG 
GTAAACTGCT CTTCGTCCTC CATATCGTCG TTGTCCTGGT CAACGGGATC CAAGGAGCCC 
TCTTCTGGCA AACAAGCAAT TTTTAGAGAG CAGTCCTTAA ATGGGGAATA CCACATCCCA 
CCTGAGGAGG AGGTGGAGTA CGAGGAAGTT GGCGCTTTGC TGCTTCGGCA TGCACCACCC 
ATTGCAGAAC AACAGCGCCC GCAGTCAGAA AACTTAAGTG CCGTGCGTCT AATCTACAAG 
GAAGAGCCAA GGCAAAGCAA GAATCATGTT CGTTACGGTC GCCTGCTTCC CTCCAAGCTA 
GCACCGGCTC AGCATGGCGA GTCACTGGAT AATTTGACAG CTACACCGCC TATTCCACCC 
GCTTCACTTA CCGGCTCGCG TATTCGAACT CCACGAGGAA TTCAGCGCAA TAATTTTATT 
TCGGGACCCC GGCCACACAG CTTGCCCTCG CCAGAAAGCG CTTATTCCAC GGGCTACTCC 
ACAGATGGCA CATCGCCATG CGCCGCCAGC AACTACAATC CACCTGAGTA CTACATTAAC 
ATGCGCTCGG GAACCCACTA TTTCCCGAAG AGCGTCAACT CGCTGGCCGT CGAGGCCCAG 
CGGTACAAGT TCGGCCTTAA CCGCATAGAG GAGATGTCGC CTATGGATCC GTTGCCCAAG 
AGCAACTTCG CAACTGCCAC TCATGGCCCG GAGTCGTCGC CTAGTTCGCT AGCTCTGCGG 
CAGCAGTCAA AGCTCTTTGA GTCTCCCTCG CCGAGACAGC GCTGTCGTAT AAGAACAAAT 
CCCTGGTACA ACACGGGAGA GCTCTTGCCT AGCACGACAG GCCCCACACG CTTGGATCTG 
GATGCGACCA GTACTACCTC CACGACCTCG TCGGGCATTA AGTCAAGCAA TGAACTGGAA 
GTTCCGGTGG CCAAAACAAT AGCAAACAAA GCAGGGAAGC CAGTCCCTTA TGCAGCAACC 
TCCAACTGCA GAGCTACTCC TAGCCAGGCA GCCAAACAGG CTGATATAGA CATTGTTGGG 
GGAGGGGCTG GTGTTGCACC TGGGGCTGGA GTTGTTTATG AATCCGAGGG CTCCTCGACT 
TCGACTGAAT TGGAAAATGT GCATGCCCCA CATACCATTA AGCGGCGTCA CTTGGACCAC 
GCCGAGACTA CAACAACTTC AGCGCCCGTG GCAGCTGGTG GTGGAACTGG AGCTACCACT 
ACATCCTTCG TTCTTAGTGA TGACGAGGCC ACCCTTAATG AAATGATTGG GAAGTTCGAC 
GAGAGTTACA TTTACGAAAA GGAAACTGAT ATATTAAGCA GTGATTCAGA CCTTACGGAC 
TGCTGTGCTT CGGAGCTAGA TACTGGACAG GATGCGGGTG ATGAGTGTGA CACAGACGAG 
TTGTTGGACA TTGACTTTAT AGACACTTCA TCCATGCAAG AGGTGGAACT TGACCGCAAG 
GATCTGTCAC GAAATCTGGG CAACTGTCAC TACTATTCTA ATCACCCTAT CAGTCCGGTG 
CTCAATCGAA AGGCATCAGT CCGTTCCCGA CGCAAGAGCG GTCAGGAGAC GGGGGAAAGC 
TCCCAACAGC GCCGACGCAA GAGGTTCCTT AAAACACGTA AGAAGAGTTG TGAAAATCGC 
GAAAAGACTT CTTCGTCACC TCTTCGTGAG CCTCGTGGCA CCCGAAGTGT GGGTGGCACG 
CCCGTCTGTT TGCGCCGCAA TCTACTGGGT CCAAGTGAGA GAATATACAA AACGTCTCCC 
CTGTCAAACC GCTCCAACTC GCTAATCTTT GGAAGCATAA AAAAAAAGTC AACACTCACC 
ATCGCAGAAA GCGAAAAAGC ACTTCTTAAG GCAGACTTGG AGGCTGATAT GAAATATAAA 
CAACTAATTA TGGAAGCGGA GTCTTTGCTG GAATCGATGA AGAACAGCTT ACAGAGCATT 
CCCAGAGACA CGCCTGTCGC CAGCCCAAGA CGAGTGAATC CCTTAGCTAA CAAGCGGGTG 
GAAATGCTTA AAAACAGTGA AGTGGTGGAC TCCAAAAAAC AGACTCTGGT TCTTAACTCT 
CCTCCTCTTG AGCATGAGTT AGCAGCAGCC ATTAATAAGC GCATAGAGAT GCTCAAGTTT 
GAGACATCGT CTGCTTCTTC GCCACCAAAC AGTCCCAAGC CTGCCCGTTA CAGTCCTCGC 
AAAACGCATC TGACCAACTT TATGAATCAA AATGCTCCAC CAGAGTTGCC ACCACGAAAA 
ATAAAAGGCA ACCCTCCCTC TGCTCCACTC TCGCCACAAC TTCAGCTAAA TGGAAGGAGT 
ATTCAGGAGA GTCCAAAGAG CAAGCGACGC ACGATGATGG TGACTTCTTC TACCACGACA 
ACGGTGATCA GCTTCAACGG CAGTGATCCT GAGATGGAGT GCATAGCTGT AGAGGATATT 
GGCAATCACA ACTACTTGCC TCAGCCGCAA TCGAATACGA GGGATGGAAA ATATGGATCG 
AATACGAAAA TGGAAAACCC ATTGCATGCC GAATTAAATA TTAATAACAA CTTTTACTGC 
CCACACAGCG AGCCCTTAAA GCGCAAGGTA TACAAGGGTA GCTCATCGTT CGAGCGCATA 
AAAAAAAACT TCGACTTCGA ATTGGATTCA AACGGACGGT GCAAGTCAAA TGAGCGTTCG 
CAAATTAAGC TTTTGGCCTC CGTGTCGGCC AAGAGCTCTA TGCAAGACGT GACCGTTGAC 
GAGGCCAAGG GCCATGCAAT AGGCGGTAGT AACCGCAAGC AGCAGCTCAT ACTGAATACC 
ATTTTCGATT TAAAGCGCAG CTTGGAGCAT CAAAGCCATC AACTGAGTGG ATTTAATGGC 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG12851 ( NM_001299937.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286866.1 Drosophila melanogaster uncharacterized protein, transcript variant D (CG12851), mRNA.

Price & Availability↑

CloneID	OFa11269
Clone ID Related Accession (Same CDS sequence)	NM_138143.4 , NM_001299937.1
Accession Version	NM_001299937.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTGGAT CCAAGCTGCG CTCCTGGATG GAGACGCATA TTGTGCGTCC GCGGAAGAAG 
GGCAGTAAAC ACAAGCAAGG GACCGTGGAC GCAGGCAGTT CTTGCGTGGG GAAGAAGGCG 
GGCACCTTAC CAACACAGGG CAGCAACCTA ACTAGCCCGG TCCTGACCAG TAGCGGAAGC 
CACAGCATCG CCAGTCCGGT GCGGAGGCGC GAGGTCTCCC CCATCCAGTG TCACCCACCC 
AGTCGTCGAT ATGGCTGGCA ATCCCCAGAT GAAGATTCCT ACGTCATAGC CTCGCCCAAG 
TCGGACAATT TGCTTCACGC TCCGCACTGC TATCAGCCGC ATCAACCGCT CCAGCAGCCG 
TCGCACCACT TAAGCGTGCC CAATAGCAAG GAAACCTATG CGGAGAAATC GCCCACCAGG 
GTAAACTGCT CTTCGTCCTC CATATCGTCG TTGTCCTGGT CAACGGGATC CAAGGAGCCC 
TCTTCTGGCA AACAAGCAAT TTTTAGAGAG CAGTCCTTAA ATGGGGAATA CCACATCCCA 
CCTGAGGAGG AGGTGGAGTA CGAGGAAGTT GGCGCTTTGC TGCTTCGGCA TGCACCACCC 
ATTGCAGAAC AACAGCGCCC GCAGTCAGAA AACTTAAGTG CCGTGCGTCT AATCTACAAG 
GAAGAGCCAA GGCAAAGCAA GAATCATGTT CGTTACGGTC GCCTGCTTCC CTCCAAGCTA 
GCACCGGCTC AGCATGGCGA GTCACTGGAT AATTTGACAG CTACACCGCC TATTCCACCC 
GCTTCACTTA CCGGCTCGCG TATTCGAACT CCACGAGGAA TTCAGCGCAA TAATTTTATT 
TCGGGACCCC GGCCACACAG CTTGCCCTCG CCAGAAAGCG CTTATTCCAC GGGCTACTCC 
ACAGATGGCA CATCGCCATG CGCCGCCAGC AACTACAATC CACCTGAGTA CTACATTAAC 
ATGCGCTCGG GAACCCACTA TTTCCCGAAG AGCGTCAACT CGCTGGCCGT CGAGGCCCAG 
CGGTACAAGT TCGGCCTTAA CCGCATAGAG GAGATGTCGC CTATGGATCC GTTGCCCAAG 
AGCAACTTCG CAACTGCCAC TCATGGCCCG GAGTCGTCGC CTAGTTCGCT AGCTCTGCGG 
CAGCAGTCAA AGCTCTTTGA GTCTCCCTCG CCGAGACAGC GCTGTCGTAT AAGAACAAAT 
CCCTGGTACA ACACGGGAGA GCTCTTGCCT AGCACGACAG GCCCCACACG CTTGGATCTG 
GATGCGACCA GTACTACCTC CACGACCTCG TCGGGCATTA AGTCAAGCAA TGAACTGGAA 
GTTCCGGTGG CCAAAACAAT AGCAAACAAA GCAGGGAAGC CAGTCCCTTA TGCAGCAACC 
TCCAACTGCA GAGCTACTCC TAGCCAGGCA GCCAAACAGG CTGATATAGA CATTGTTGGG 
GGAGGGGCTG GTGTTGCACC TGGGGCTGGA GTTGTTTATG AATCCGAGGG CTCCTCGACT 
TCGACTGAAT TGGAAAATGT GCATGCCCCA CATACCATTA AGCGGCGTCA CTTGGACCAC 
GCCGAGACTA CAACAACTTC AGCGCCCGTG GCAGCTGGTG GTGGAACTGG AGCTACCACT 
ACATCCTTCG TTCTTAGTGA TGACGAGGCC ACCCTTAATG AAATGATTGG GAAGTTCGAC 
GAGAGTTACA TTTACGAAAA GGAAACTGAT ATATTAAGCA GTGATTCAGA CCTTACGGAC 
TGCTGTGCTT CGGAGCTAGA TACTGGACAG GATGCGGGTG ATGAGTGTGA CACAGACGAG 
TTGTTGGACA TTGACTTTAT AGACACTTCA TCCATGCAAG AGGTGGAACT TGACCGCAAG 
GATCTGTCAC GAAATCTGGG CAACTGTCAC TACTATTCTA ATCACCCTAT CAGTCCGGTG 
CTCAATCGAA AGGCATCAGT CCGTTCCCGA CGCAAGAGCG GTCAGGAGAC GGGGGAAAGC 
TCCCAACAGC GCCGACGCAA GAGGTTCCTT AAAACACGTA AGAAGAGTTG TGAAAATCGC 
GAAAAGACTT CTTCGTCACC TCTTCGTGAG CCTCGTGGCA CCCGAAGTGT GGGTGGCACG 
CCCGTCTGTT TGCGCCGCAA TCTACTGGGT CCAAGTGAGA GAATATACAA AACGTCTCCC 
CTGTCAAACC GCTCCAACTC GCTAATCTTT GGAAGCATAA AAAAAAAGTC AACACTCACC 
ATCGCAGAAA GCGAAAAAGC ACTTCTTAAG GCAGACTTGG AGGCTGATAT GAAATATAAA 
CAACTAATTA TGGAAGCGGA GTCTTTGCTG GAATCGATGA AGAACAGCTT ACAGAGCATT 
CCCAGAGACA CGCCTGTCGC CAGCCCAAGA CGAGTGAATC CCTTAGCTAA CAAGCGGGTG 
GAAATGCTTA AAAACAGTGA AGTGGTGGAC TCCAAAAAAC AGACTCTGGT TCTTAACTCT 
CCTCCTCTTG AGCATGAGTT AGCAGCAGCC ATTAATAAGC GCATAGAGAT GCTCAAGTTT 
GAGACATCGT CTGCTTCTTC GCCACCAAAC AGTCCCAAGC CTGCCCGTTA CAGTCCTCGC 
AAAACGCATC TGACCAACTT TATGAATCAA AATGCTCCAC CAGAGTTGCC ACCACGAAAA 
ATAAAAGGCA ACCCTCCCTC TGCTCCACTC TCGCCACAAC TTCAGCTAAA TGGAAGGAGT 
ATTCAGGAGA GTCCAAAGAG CAAGCGACGC ACGATGATGG TGACTTCTTC TACCACGACA 
ACGGTGATCA GCTTCAACGG CAGTGATCCT GAGATGGAGT GCATAGCTGT AGAGGATATT 
GGCAATCACA ACTACTTGCC TCAGCCGCAA TCGAATACGA GGGATGGAAA ATATGGATCG 
AATACGAAAA TGGAAAACCC ATTGCATGCC GAATTAAATA TTAATAACAA CTTTTACTGC 
CCACACAGCG AGCCCTTAAA GCGCAAGGTA TACAAGGGTA GCTCATCGTT CGAGCGCATA 
AAAAAAAACT TCGACTTCGA ATTGGATTCA AACGGACGGT GCAAGTCAAA TGAGCGTTCG 
CAAATTAAGC TTTTGGCCTC CGTGTCGGCC AAGAGCTCTA TGCAAGACGT GACCGTTGAC 
GAGGCCAAGG GCCATGCAAT AGGCGGTAGT AACCGCAAGC AGCAGCTCAT ACTGAATACC 
ATTTTCGATT TAAAGCGCAG CTTGGAGCAT CAAAGCCATC AACTGAGTGG ATTTAATGGC 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286866.1
CDS	801..4046
Translation	MFGSKLRSWMETHIVRPRKKGSKHKQGTVDAGSSCVGKKAGTLPTQGSNLTSPVLTSSGSHSIASPVRRREVSPIQCHPPSRRYGWQSPDEDSYVIASPKSDNLLHAPHCYQPHQPLQQPSHHLSVPNSKETYAEKSPTRVNCSSSSISSLSWSTGSKEPSSGKQAIFREQSLNGEYHIPPEEEVEYEEVGALLLRHAPPIAEQQRPQSENLSAVRLIYKEEPRQSKNHVRYGRLLPSKLAPAQHGESLDNLTATPPIPPASLTGSRIRTPRGIQRNNFISGPRPHSLPSPESAYSTGYSTDGTSPCAASNYNPPEYYINMRSGTHYFPKSVNSLAVEAQRYKFGLNRIEEMSPMDPLPKSNFATATHGPESSPSSLALRQQSKLFESPSPRQRCRIRTNPWYNTGELLPSTTGPTRLDLDATSTTSTTSSGIKSSNELEVPVAKTIANKAGKPVPYAATSNCRATPSQAAKQADIDIVGGGAGVAPGAGVVYESEGSSTSTELENVHAPHTIKRRHLDHAETTTTSAPVAAGGGTGATTTSFVLSDDEATLNEMIGKFDESYIYEKETDILSSDSDLTDCCASELDTGQDAGDECDTDELLDIDFIDTSSMQEVELDRKDLSRNLGNCHYYSNHPISPVLNRKASVRSRRKSGQETGESSQQRRRKRFLKTRKKSCENREKTSSSPLREPRGTRSVGGTPVCLRRNLLGPSERIYKTSPLSNRSNSLIFGSIKKKSTLTIAESEKALLKADLEADMKYKQLIMEAESLLESMKNSLQSIPRDTPVASPRRVNPLANKRVEMLKNSEVVDSKKQTLVLNSPPLEHELAAAINKRIEMLKFETSSASSPPNSPKPARYSPRKTHLTNFMNQNAPPELPPRKIKGNPPSAPLSPQLQLNGRSIQESPKSKRRTMMVTSSTTTTVISFNGSDPEMECIAVEDIGNHNYLPQPQSNTRDGKYGSNTKMENPLHAELNINNNFYCPHSEPLKRKVYKGSSSFERIKKNFDFELDSNGRCKSNERSQIKLLASVSAKSSMQDVTVDEAKGHAIGGSNRKQQLILNTIFDLKRSLEHQSHQLSGFNGE

Target ORF information:

RefSeq Version	NM_001299937.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant D (CG12851), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299937.1

ORF Insert Sequence:

ATGTTTGGAT CCAAGCTGCG CTCCTGGATG GAGACGCATA TTGTGCGTCC GCGGAAGAAG 
GGCAGTAAAC ACAAGCAAGG GACCGTGGAC GCAGGCAGTT CTTGCGTGGG GAAGAAGGCG 
GGCACCTTAC CAACACAGGG CAGCAACCTA ACTAGCCCGG TCCTGACCAG TAGCGGAAGC 
CACAGCATCG CCAGTCCGGT GCGGAGGCGC GAGGTCTCCC CCATCCAGTG TCACCCACCC 
AGTCGTCGAT ATGGCTGGCA ATCCCCAGAT GAAGATTCCT ACGTCATAGC CTCGCCCAAG 
TCGGACAATT TGCTTCACGC TCCGCACTGC TATCAGCCGC ATCAACCGCT CCAGCAGCCG 
TCGCACCACT TAAGCGTGCC CAATAGCAAG GAAACCTATG CGGAGAAATC GCCCACCAGG 
GTAAACTGCT CTTCGTCCTC CATATCGTCG TTGTCCTGGT CAACGGGATC CAAGGAGCCC 
TCTTCTGGCA AACAAGCAAT TTTTAGAGAG CAGTCCTTAA ATGGGGAATA CCACATCCCA 
CCTGAGGAGG AGGTGGAGTA CGAGGAAGTT GGCGCTTTGC TGCTTCGGCA TGCACCACCC 
ATTGCAGAAC AACAGCGCCC GCAGTCAGAA AACTTAAGTG CCGTGCGTCT AATCTACAAG 
GAAGAGCCAA GGCAAAGCAA GAATCATGTT CGTTACGGTC GCCTGCTTCC CTCCAAGCTA 
GCACCGGCTC AGCATGGCGA GTCACTGGAT AATTTGACAG CTACACCGCC TATTCCACCC 
GCTTCACTTA CCGGCTCGCG TATTCGAACT CCACGAGGAA TTCAGCGCAA TAATTTTATT 
TCGGGACCCC GGCCACACAG CTTGCCCTCG CCAGAAAGCG CTTATTCCAC GGGCTACTCC 
ACAGATGGCA CATCGCCATG CGCCGCCAGC AACTACAATC CACCTGAGTA CTACATTAAC 
ATGCGCTCGG GAACCCACTA TTTCCCGAAG AGCGTCAACT CGCTGGCCGT CGAGGCCCAG 
CGGTACAAGT TCGGCCTTAA CCGCATAGAG GAGATGTCGC CTATGGATCC GTTGCCCAAG 
AGCAACTTCG CAACTGCCAC TCATGGCCCG GAGTCGTCGC CTAGTTCGCT AGCTCTGCGG 
CAGCAGTCAA AGCTCTTTGA GTCTCCCTCG CCGAGACAGC GCTGTCGTAT AAGAACAAAT 
CCCTGGTACA ACACGGGAGA GCTCTTGCCT AGCACGACAG GCCCCACACG CTTGGATCTG 
GATGCGACCA GTACTACCTC CACGACCTCG TCGGGCATTA AGTCAAGCAA TGAACTGGAA 
GTTCCGGTGG CCAAAACAAT AGCAAACAAA GCAGGGAAGC CAGTCCCTTA TGCAGCAACC 
TCCAACTGCA GAGCTACTCC TAGCCAGGCA GCCAAACAGG CTGATATAGA CATTGTTGGG 
GGAGGGGCTG GTGTTGCACC TGGGGCTGGA GTTGTTTATG AATCCGAGGG CTCCTCGACT 
TCGACTGAAT TGGAAAATGT GCATGCCCCA CATACCATTA AGCGGCGTCA CTTGGACCAC 
GCCGAGACTA CAACAACTTC AGCGCCCGTG GCAGCTGGTG GTGGAACTGG AGCTACCACT 
ACATCCTTCG TTCTTAGTGA TGACGAGGCC ACCCTTAATG AAATGATTGG GAAGTTCGAC 
GAGAGTTACA TTTACGAAAA GGAAACTGAT ATATTAAGCA GTGATTCAGA CCTTACGGAC 
TGCTGTGCTT CGGAGCTAGA TACTGGACAG GATGCGGGTG ATGAGTGTGA CACAGACGAG 
TTGTTGGACA TTGACTTTAT AGACACTTCA TCCATGCAAG AGGTGGAACT TGACCGCAAG 
GATCTGTCAC GAAATCTGGG CAACTGTCAC TACTATTCTA ATCACCCTAT CAGTCCGGTG 
CTCAATCGAA AGGCATCAGT CCGTTCCCGA CGCAAGAGCG GTCAGGAGAC GGGGGAAAGC 
TCCCAACAGC GCCGACGCAA GAGGTTCCTT AAAACACGTA AGAAGAGTTG TGAAAATCGC 
GAAAAGACTT CTTCGTCACC TCTTCGTGAG CCTCGTGGCA CCCGAAGTGT GGGTGGCACG 
CCCGTCTGTT TGCGCCGCAA TCTACTGGGT CCAAGTGAGA GAATATACAA AACGTCTCCC 
CTGTCAAACC GCTCCAACTC GCTAATCTTT GGAAGCATAA AAAAAAAGTC AACACTCACC 
ATCGCAGAAA GCGAAAAAGC ACTTCTTAAG GCAGACTTGG AGGCTGATAT GAAATATAAA 
CAACTAATTA TGGAAGCGGA GTCTTTGCTG GAATCGATGA AGAACAGCTT ACAGAGCATT 
CCCAGAGACA CGCCTGTCGC CAGCCCAAGA CGAGTGAATC CCTTAGCTAA CAAGCGGGTG 
GAAATGCTTA AAAACAGTGA AGTGGTGGAC TCCAAAAAAC AGACTCTGGT TCTTAACTCT 
CCTCCTCTTG AGCATGAGTT AGCAGCAGCC ATTAATAAGC GCATAGAGAT GCTCAAGTTT 
GAGACATCGT CTGCTTCTTC GCCACCAAAC AGTCCCAAGC CTGCCCGTTA CAGTCCTCGC 
AAAACGCATC TGACCAACTT TATGAATCAA AATGCTCCAC CAGAGTTGCC ACCACGAAAA 
ATAAAAGGCA ACCCTCCCTC TGCTCCACTC TCGCCACAAC TTCAGCTAAA TGGAAGGAGT 
ATTCAGGAGA GTCCAAAGAG CAAGCGACGC ACGATGATGG TGACTTCTTC TACCACGACA 
ACGGTGATCA GCTTCAACGG CAGTGATCCT GAGATGGAGT GCATAGCTGT AGAGGATATT 
GGCAATCACA ACTACTTGCC TCAGCCGCAA TCGAATACGA GGGATGGAAA ATATGGATCG 
AATACGAAAA TGGAAAACCC ATTGCATGCC GAATTAAATA TTAATAACAA CTTTTACTGC 
CCACACAGCG AGCCCTTAAA GCGCAAGGTA TACAAGGGTA GCTCATCGTT CGAGCGCATA 
AAAAAAAACT TCGACTTCGA ATTGGATTCA AACGGACGGT GCAAGTCAAA TGAGCGTTCG 
CAAATTAAGC TTTTGGCCTC CGTGTCGGCC AAGAGCTCTA TGCAAGACGT GACCGTTGAC 
GAGGCCAAGG GCCATGCAAT AGGCGGTAGT AACCGCAAGC AGCAGCTCAT ACTGAATACC 
ATTTTCGATT TAAAGCGCAG CTTGGAGCAT CAAAGCCATC AACTGAGTGG ATTTAATGGC 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG12851 ( NM_001299936.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286865.1 Drosophila melanogaster uncharacterized protein, transcript variant C (CG12851), mRNA.

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CloneID	OFa21959
Clone ID Related Accession (Same CDS sequence)	NM_001299936.1
Accession Version	NM_001299936.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTGGAT CCAAGCTGCG CTCCTGGATG GAGACGCATA TTGTGCGTCC GCGGAAGAAG 
GGCAGTAAAC ACAAGCAAGG GACCGTGGAC GCAGGCAGTT CTTGCGTGGG GAAGAAGGCG 
GGCACCTTAC CAACACAGGG CAGCAACCTA ACTAGCCCGG TCCTGACCAG TAGCGGAAGC 
CACAGCATCG CCAGTCCGGT GCGGAGGCGC GAGGTCTCCC CCATCCAGTG TCACCCACCC 
AGTCGTCGAT ATGGCTGGCA ATCCCCAGAT GAAGATTCCT ACGTCATAGC CTCGCCCAAG 
TCGGACAATT TGCTTCACGC TCCGCACTGC TATCAGCCGC ATCAACCGCT CCAGCAGCCG 
TCGCACCACT TAAGCGTGCC CAATAGCAAG GAAACCTATG CGGAGAAATC GCCCACCAGG 
GTAAACTGCT CTTCGTCCTC CATATCGTCG TTGTCCTGGT CAACGGGATC CAAGGAGCCC 
TCTTCTGGCA AACAAGCAAT TTTTAGAGAG CAGTCCTTAA ATGGGGAATA CCACATCCCA 
CCTGAGGAGG AGGTGGAGTA CGAGGAAGTT GGCGCTTTGC TGCTTCGGCA TGCACCACCC 
ATTGCAGAAC AACAGCGCCC GCAGTCAGAA AACTTAAGTG CCGTGCGTCT AATCTACAAG 
GAAGAGCCAA GGCAAAGCAA GAATCATGTT CGTTACGGTC GCCTGCTTCC CTCCAAGCTA 
GCACCGGCTC AGCATGGCGA GTCACTGGAT AATTTGACAG CTACACCGCC TATTCCACCC 
GCTTCACTTA CCGGCTCGCG TATTCGAACT CCACGAGGAA TTCAGCGCAA TAATTTTATT 
TCGGGACCCC GGCCACACAG CTTGCCCTCG CCAGAAAGCG CTTATTCCAC GGGCTACTCC 
ACAGATGGCA CATCGCCATG CGCCGCCAGC AACTACAATC CACCTGAGTA CTACATTAAC 
ATGCGCTCGG GAACCCACTA TTTCCCGAAG AGCGTCAACT CGCTGGCCGT CGAGGCCCAG 
CGGTACAAGT TCGGCCTTAA CCGCATAGAG GAGATGTCGC CTATGGATCC GTTGCCCAAG 
AGCAACTTCG CAACTGCCAC TCATGGCCCG GAGTCGTCGC CTAGTTCGCT AGCTCTGCGG 
CAGCAGTCAA AGCTCTTTGA GTCTCCCTCG CCGAGACAGC GCTGTCGTAT AAGAACAAAT 
CCCTGGTACA ACACGGGAGA GCTCTTGCCT AGCACGACAG GCCCCACACG CTTGGATCTG 
GATGCGACCA GTACTACCTC CACGACCTCG TCGGGCATTA AGTCAAGCAA TGAACTGGAA 
GTTCCGGTGG CCAAAACAAT AGCAAACAAA GCAGGGAAGC CAGTCCCTTA TGCAGCAACC 
TCCAACTGCA GAGCTACTCC TAGCCAGGCA GCCAAACAGG CTGATATAGA CATTGTTGGG 
GGAGGGGCTG GTGTTGCACC TGGGGCTGGA GTTGTTTATG AATCCGAGGG CTCCTCGACT 
TCGACTGAAT TGGAAAATGT GCATGCCCCA CATACCATTA AGCGGCGTCA CTTGGACCAC 
GCCGAGACTA CAACAACTTC AGCGCCCGTG GCAGCTGGTG GTGGAACTGG AGCTACCACT 
ACATCCTTCG TTCTTAGTGA TGACGAGGCC ACCCTTAATG AAATGATTGG GAAGTTCGAC 
GAGAGTTACA TTTACGAAAA GGAAACTGAT ATATTAAGTG ATTCAGACCT TACGGACTGC 
TGTGCTTCGG AGCTAGATAC TGGACAGGAT GCGGGTGATG AGTGTGACAC AGACGAGTTG 
TTGGACATTG ACTTTATAGA CACTTCATCC ATGCAAGAGG TGGAACTTGA CCGCAAGGAT 
CTGTCACGAA ATCTGGGCAA CTGTCACTAC TATTCTAATC ACCCTATCAG TCCGGTGCTC 
AATCGAAAGG CATCAGTCCG TTCCCGACGC AAGAGCGGTC AGGAGACGGG GGAAAGCTCC 
CAACAGCGCC GACGCAAGAG GTTCCTTAAA ACACGTAAGA AGAGTTGTGA AAATCGCGAA 
AAGACTTCTT CGTCACCTCT TCGTGAGCCT CGTGGCACCC GAAGTGTGGG TGGCACGCCC 
GTCTGTTTGC GCCGCAATCT ACTGGGTCCA AGTGAGAGAA TATACAAAAC GTCTCCCCTG 
TCAAACCGCT CCAACTCGCT AATCTTTGGA AGCATAAAAA AAAAGTCAAC ACTCACCATC 
GCAGAAAGCG AAAAAGCACT TCTTAAGGCA GACTTGGAGG CTGATATGAA ATATAAACAA 
CTAATTATGG AAGCGGAGTC TTTGCTGGAA TCGATGAAGA ACAGCTTACA GAGCATTCCC 
AGAGACACGC CTGTCGCCAG CCCAAGACGA GTGAATCCCT TAGCTAACAA GCGGGTGGAA 
ATGCTTAAAA ACAGTGAAGT GGTGGACTCC AAAAAACAGA CTCTGGTTCT TAACTCTCCT 
CCTCTTGAGC ATGAGTTAGC AGCAGCCATT AATAAGCGCA TAGAGATGCT CAAGTTTGAG 
ACATCGTCTG CTTCTTCGCC ACCAAACAGT CCCAAGCCTG CCCGTTACAG TCCTCGCAAA 
ACGCATCTGA CCAACTTTAT GAATCAAAAT GCTCCACCAG AGTTGCCACC ACGAAAAATA 
AAAGGCAACC CTCCCTCTGC TCCACTCTCG CCACAACTTC AGCTAAATGG AAGGAGTATT 
CAGGAGAGTC CAAAGAGCAA GCGACGCACG ATGATGGTGA CTTCTTCTAC CACGACAACG 
GTGATCAGCT TCAACGGCAG TGATCCTGAG ATGGAGTGCA TAGCTGTAGA GGATATTGGC 
AATCACAACT ACTTGCCTCA GCCGCAATCG AATACGAGGG ATGGAAAATA TGGATCGAAT 
ACGAAAATGG AAAACCCATT GCATGCCGAA TTAAATATTA ATAACAACTT TTACTGCCCA 
CACAGCGAGC CCTTAAAGCG CAAGGTATAC AAGGGTAGCT CATCGTTCGA GCGCATAAAA 
AAAAACTTCG ACTTCGAATT GGATTCAAAC GGACGGTGCA AGTCAAATGA GCGTTCGCAA 
ATTAAGCTTT TGGCCTCCGT GTCGGCCAAG AGCTCTATGC AAGACGTGAC CGTTGACGAG 
GCCAAGGGCC ATGCAATAGG CGGTAGTAAC CGCAAGCAGC AGCTCATACT GAATACCATT 
TTCGATTTAA AGCGCAGCTT GGAGCATCAA AGCCATCAAC TGAGTGGATT TAATGGCGAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286865.1
CDS	801..4043
Translation	MFGSKLRSWMETHIVRPRKKGSKHKQGTVDAGSSCVGKKAGTLPTQGSNLTSPVLTSSGSHSIASPVRRREVSPIQCHPPSRRYGWQSPDEDSYVIASPKSDNLLHAPHCYQPHQPLQQPSHHLSVPNSKETYAEKSPTRVNCSSSSISSLSWSTGSKEPSSGKQAIFREQSLNGEYHIPPEEEVEYEEVGALLLRHAPPIAEQQRPQSENLSAVRLIYKEEPRQSKNHVRYGRLLPSKLAPAQHGESLDNLTATPPIPPASLTGSRIRTPRGIQRNNFISGPRPHSLPSPESAYSTGYSTDGTSPCAASNYNPPEYYINMRSGTHYFPKSVNSLAVEAQRYKFGLNRIEEMSPMDPLPKSNFATATHGPESSPSSLALRQQSKLFESPSPRQRCRIRTNPWYNTGELLPSTTGPTRLDLDATSTTSTTSSGIKSSNELEVPVAKTIANKAGKPVPYAATSNCRATPSQAAKQADIDIVGGGAGVAPGAGVVYESEGSSTSTELENVHAPHTIKRRHLDHAETTTTSAPVAAGGGTGATTTSFVLSDDEATLNEMIGKFDESYIYEKETDILSDSDLTDCCASELDTGQDAGDECDTDELLDIDFIDTSSMQEVELDRKDLSRNLGNCHYYSNHPISPVLNRKASVRSRRKSGQETGESSQQRRRKRFLKTRKKSCENREKTSSSPLREPRGTRSVGGTPVCLRRNLLGPSERIYKTSPLSNRSNSLIFGSIKKKSTLTIAESEKALLKADLEADMKYKQLIMEAESLLESMKNSLQSIPRDTPVASPRRVNPLANKRVEMLKNSEVVDSKKQTLVLNSPPLEHELAAAINKRIEMLKFETSSASSPPNSPKPARYSPRKTHLTNFMNQNAPPELPPRKIKGNPPSAPLSPQLQLNGRSIQESPKSKRRTMMVTSSTTTTVISFNGSDPEMECIAVEDIGNHNYLPQPQSNTRDGKYGSNTKMENPLHAELNINNNFYCPHSEPLKRKVYKGSSSFERIKKNFDFELDSNGRCKSNERSQIKLLASVSAKSSMQDVTVDEAKGHAIGGSNRKQQLILNTIFDLKRSLEHQSHQLSGFNGE

Target ORF information:

RefSeq Version	NM_001299936.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant C (CG12851), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299936.1

ORF Insert Sequence:

ATGTTTGGAT CCAAGCTGCG CTCCTGGATG GAGACGCATA TTGTGCGTCC GCGGAAGAAG 
GGCAGTAAAC ACAAGCAAGG GACCGTGGAC GCAGGCAGTT CTTGCGTGGG GAAGAAGGCG 
GGCACCTTAC CAACACAGGG CAGCAACCTA ACTAGCCCGG TCCTGACCAG TAGCGGAAGC 
CACAGCATCG CCAGTCCGGT GCGGAGGCGC GAGGTCTCCC CCATCCAGTG TCACCCACCC 
AGTCGTCGAT ATGGCTGGCA ATCCCCAGAT GAAGATTCCT ACGTCATAGC CTCGCCCAAG 
TCGGACAATT TGCTTCACGC TCCGCACTGC TATCAGCCGC ATCAACCGCT CCAGCAGCCG 
TCGCACCACT TAAGCGTGCC CAATAGCAAG GAAACCTATG CGGAGAAATC GCCCACCAGG 
GTAAACTGCT CTTCGTCCTC CATATCGTCG TTGTCCTGGT CAACGGGATC CAAGGAGCCC 
TCTTCTGGCA AACAAGCAAT TTTTAGAGAG CAGTCCTTAA ATGGGGAATA CCACATCCCA 
CCTGAGGAGG AGGTGGAGTA CGAGGAAGTT GGCGCTTTGC TGCTTCGGCA TGCACCACCC 
ATTGCAGAAC AACAGCGCCC GCAGTCAGAA AACTTAAGTG CCGTGCGTCT AATCTACAAG 
GAAGAGCCAA GGCAAAGCAA GAATCATGTT CGTTACGGTC GCCTGCTTCC CTCCAAGCTA 
GCACCGGCTC AGCATGGCGA GTCACTGGAT AATTTGACAG CTACACCGCC TATTCCACCC 
GCTTCACTTA CCGGCTCGCG TATTCGAACT CCACGAGGAA TTCAGCGCAA TAATTTTATT 
TCGGGACCCC GGCCACACAG CTTGCCCTCG CCAGAAAGCG CTTATTCCAC GGGCTACTCC 
ACAGATGGCA CATCGCCATG CGCCGCCAGC AACTACAATC CACCTGAGTA CTACATTAAC 
ATGCGCTCGG GAACCCACTA TTTCCCGAAG AGCGTCAACT CGCTGGCCGT CGAGGCCCAG 
CGGTACAAGT TCGGCCTTAA CCGCATAGAG GAGATGTCGC CTATGGATCC GTTGCCCAAG 
AGCAACTTCG CAACTGCCAC TCATGGCCCG GAGTCGTCGC CTAGTTCGCT AGCTCTGCGG 
CAGCAGTCAA AGCTCTTTGA GTCTCCCTCG CCGAGACAGC GCTGTCGTAT AAGAACAAAT 
CCCTGGTACA ACACGGGAGA GCTCTTGCCT AGCACGACAG GCCCCACACG CTTGGATCTG 
GATGCGACCA GTACTACCTC CACGACCTCG TCGGGCATTA AGTCAAGCAA TGAACTGGAA 
GTTCCGGTGG CCAAAACAAT AGCAAACAAA GCAGGGAAGC CAGTCCCTTA TGCAGCAACC 
TCCAACTGCA GAGCTACTCC TAGCCAGGCA GCCAAACAGG CTGATATAGA CATTGTTGGG 
GGAGGGGCTG GTGTTGCACC TGGGGCTGGA GTTGTTTATG AATCCGAGGG CTCCTCGACT 
TCGACTGAAT TGGAAAATGT GCATGCCCCA CATACCATTA AGCGGCGTCA CTTGGACCAC 
GCCGAGACTA CAACAACTTC AGCGCCCGTG GCAGCTGGTG GTGGAACTGG AGCTACCACT 
ACATCCTTCG TTCTTAGTGA TGACGAGGCC ACCCTTAATG AAATGATTGG GAAGTTCGAC 
GAGAGTTACA TTTACGAAAA GGAAACTGAT ATATTAAGTG ATTCAGACCT TACGGACTGC 
TGTGCTTCGG AGCTAGATAC TGGACAGGAT GCGGGTGATG AGTGTGACAC AGACGAGTTG 
TTGGACATTG ACTTTATAGA CACTTCATCC ATGCAAGAGG TGGAACTTGA CCGCAAGGAT 
CTGTCACGAA ATCTGGGCAA CTGTCACTAC TATTCTAATC ACCCTATCAG TCCGGTGCTC 
AATCGAAAGG CATCAGTCCG TTCCCGACGC AAGAGCGGTC AGGAGACGGG GGAAAGCTCC 
CAACAGCGCC GACGCAAGAG GTTCCTTAAA ACACGTAAGA AGAGTTGTGA AAATCGCGAA 
AAGACTTCTT CGTCACCTCT TCGTGAGCCT CGTGGCACCC GAAGTGTGGG TGGCACGCCC 
GTCTGTTTGC GCCGCAATCT ACTGGGTCCA AGTGAGAGAA TATACAAAAC GTCTCCCCTG 
TCAAACCGCT CCAACTCGCT AATCTTTGGA AGCATAAAAA AAAAGTCAAC ACTCACCATC 
GCAGAAAGCG AAAAAGCACT TCTTAAGGCA GACTTGGAGG CTGATATGAA ATATAAACAA 
CTAATTATGG AAGCGGAGTC TTTGCTGGAA TCGATGAAGA ACAGCTTACA GAGCATTCCC 
AGAGACACGC CTGTCGCCAG CCCAAGACGA GTGAATCCCT TAGCTAACAA GCGGGTGGAA 
ATGCTTAAAA ACAGTGAAGT GGTGGACTCC AAAAAACAGA CTCTGGTTCT TAACTCTCCT 
CCTCTTGAGC ATGAGTTAGC AGCAGCCATT AATAAGCGCA TAGAGATGCT CAAGTTTGAG 
ACATCGTCTG CTTCTTCGCC ACCAAACAGT CCCAAGCCTG CCCGTTACAG TCCTCGCAAA 
ACGCATCTGA CCAACTTTAT GAATCAAAAT GCTCCACCAG AGTTGCCACC ACGAAAAATA 
AAAGGCAACC CTCCCTCTGC TCCACTCTCG CCACAACTTC AGCTAAATGG AAGGAGTATT 
CAGGAGAGTC CAAAGAGCAA GCGACGCACG ATGATGGTGA CTTCTTCTAC CACGACAACG 
GTGATCAGCT TCAACGGCAG TGATCCTGAG ATGGAGTGCA TAGCTGTAGA GGATATTGGC 
AATCACAACT ACTTGCCTCA GCCGCAATCG AATACGAGGG ATGGAAAATA TGGATCGAAT 
ACGAAAATGG AAAACCCATT GCATGCCGAA TTAAATATTA ATAACAACTT TTACTGCCCA 
CACAGCGAGC CCTTAAAGCG CAAGGTATAC AAGGGTAGCT CATCGTTCGA GCGCATAAAA 
AAAAACTTCG ACTTCGAATT GGATTCAAAC GGACGGTGCA AGTCAAATGA GCGTTCGCAA 
ATTAAGCTTT TGGCCTCCGT GTCGGCCAAG AGCTCTATGC AAGACGTGAC CGTTGACGAG 
GCCAAGGGCC ATGCAATAGG CGGTAGTAAC CGCAAGCAGC AGCTCATACT GAATACCATT 
TTCGATTTAA AGCGCAGCTT GGAGCATCAA AGCCATCAAC TGAGTGGATT TAATGGCGAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG12851 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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