Dll cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dll
Entrez Gene ID	37973
Full Name	Distal-less
Synonyms	2.7,Art,Ba,BcDNA:LP01770,CG3629,DLL,Dmel\CG3629,E(Arp),En(Arp),dll,l(2)01092,l(2)387
General protein information	Preferred Names Distal-less Names CG3629 gene product from transcript CG3629-RA CG3629-PA CG3629-PB CG3629-PC Distall-less Dll-PA Dll-PB Dll-PC brista distal-less distalless enhancer of Arp
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 60E2-60E2\|2-107.8 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_079133.2	NP_523857.1	Distal-less, isoform A
NM_001144287.2	NP_001137759.1	Distal-less, isoform C
NM_166689.2	NP_726486.1	Distal-less, isoform B

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007058	XP_308706.3
Drosophila melanogaster (fruit fly)	Dll	NP_001137759.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

During antennal development, expression of the selector genes Lim1 and Dll was sharply segregated. Notch signaling induces actomyosin-dependent apical constriction and epithelial fold. Disruption of Notch signaling or the actomyosin network reduces apical constriction and epithelial fold.
Title: Notch-dependent epithelial fold determines boundary formation between developmental fields in the Drosophila antenna.

we find that C15 interacts physically with the Dll activator through contacts between their homeodomain and binds competitively with Dll to adjacent cognate sites on LAE, adding potential new layers of regulation by C15. Lastly, we show that C15 and Bowl activities regulate also rn expression
Title: Tissue-specific enhancer repression through molecular integration of cell signaling inputs.

Distal-less regulatory element that controls gene expression in leg precursor cells recruits multiple Hox, Extradenticle (Exd) and Homothorax (Hth) complexes to mediate dual outputs: thoracic activation and abdominal repression.
Title: A Hox Transcription Factor Collective Binds a Highly Conserved Distal-less cis-Regulatory Module to Generate Robust Transcriptional Outcomes.

Characterization of Dll expression in the developing nervous system supports a role of Dll in neural development and function
Title: Expression of the Drosophila homeobox gene, Distal-less, supports an ancestral role in neural development.

Joint ectopic expression of Dll and Rn is sufficient to cell-autonomously activate endogenous bab2 and LAE-driven reporter expression in wing and haltere cells
Title: Drosophila distal-less and Rotund bind a single enhancer ensuring reliable and robust bric-a-brac2 expression in distinct limb morphogenetic fields.

The following Dll gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dll cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa11245	NM_079133.2 Latest version!	Drosophila melanogaster Distal-less (Dll), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa11246	NM_166689.2 Latest version!	Drosophila melanogaster Distal-less (Dll), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa13250	NM_001144287.2 Latest version!	Drosophila melanogaster Distal-less (Dll), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dll ( NM_079133.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523857.1 Drosophila melanogaster Distal-less (Dll), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa11245
Clone ID Related Accession (Same CDS sequence)	NM_079133.2
Accession Version	NM_079133.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 984bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Distal-less, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_079133.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACGCCC CCGACGCCCC GCACACGCCC AAGTACATGG ACGGCGGGAA CACGGCGGCC 
AGCGTCACGC CCGGCATTAA CATCCCGGGA AAGTCCGCCT TCGTGGAGCT GCAGCAGCAC 
GCCGCCGCCG GGTACGGCGG CATCCGGAGC ACCTATCAGC ATTTCGGACC GCAGGGCGGC 
CAGGATTCCG GCTTCCCCAG TCCGCGCAGC GCCCTCGGCT ACCCGTTCCC GCCAATGCAC 
CAGAACTCCT ACTCCGGCTA CCATCTGGGC AGCTACGCCC CGCCCTGCGC CAGTCCACCG 
AAGGATGACT TCTCCATCTC CGATAAGTGC GAGGACTCCG GCCTCCGCGT CAACGGCAAG 
GGCAAGAAGA TGCGCAAGCC CCGCACCATC TACAGCTCGC TGCAACTGCA GCAGCTCAAC 
CGCCGCTTCC AGCGTACCCA ATACCTGGCC CTGCCGGAGC GAGCGGAGCT GGCGGCGAGT 
CTGGGCCTCA CGCAAACGCA GGTGAAAATC TGGTTCCAGA ACCGACGCTC CAAGTACAAA 
AAGATGATGA AGGCGGCCCA GGGCCCGGGC ACCAACAGCG GCATGCCTCT GGGCGGGGGA 
GGACCCAATC CCGGCCAGCA TAGCCCCAAT CAAATGCACA GTGGCGGTAA TAACGGCGGT 
GGCTCGAACA GCGGCTCACC CTCACATTAT CTACCTCCCG GACACAGTCC AACGCCATCG 
AGTACACCCG TGTCCGAGCT TTCACCTGAA TTCCCACCAA CGGGCCTCAG TCCGCCGACG 
CAAGCGCCGT GGGACCAGAA ACCGCACTGG ATCGACCACA AGCCGCCGCC CCAAATGACG 
CCACAACCAC CTCATCCAGC GGCAACGCTA CATCCGCAGA CGCACCACCA CAACCCGCCG 
CCCCAGATGG GTGGCTATGT GCCCCAGTAC TGGTACCAGC CCGAGACGAA TCCATCACTA 
GTGACTGTTT GGCCGGCGGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523857.1
CDS	616..1599
Translation	MDAPDAPHTPKYMDGGNTAASVTPGINIPGKSAFVELQQHAAAGYGGIRSTYQHFGPQGGQDSGFPSPRSALGYPFPPMHQNSYSGYHLGSYAPPCASPPKDDFSISDKCEDSGLRVNGKGKKMRKPRTIYSSLQLQQLNRRFQRTQYLALPERAELAASLGLTQTQVKIWFQNRRSKYKKMMKAAQGPGTNSGMPLGGGGPNPGQHSPNQMHSGGNNGGGSNSGSPSHYLPPGHSPTPSSTPVSELSPEFPPTGLSPPTQAPWDQKPHWIDHKPPPQMTPQPPHPAATLHPQTHHHNPPPQMGGYVPQYWYQPETNPSLVTVWPAV

Target ORF information:

RefSeq Version	NM_079133.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Distal-less (Dll), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079133.2

ORF Insert Sequence:

ATGGACGCCC CCGACGCCCC GCACACGCCC AAGTACATGG ACGGCGGGAA CACGGCGGCC 
AGCGTCACGC CCGGCATTAA CATCCCGGGA AAGTCCGCCT TCGTGGAGCT GCAGCAGCAC 
GCCGCCGCCG GGTACGGCGG CATCCGGAGC ACCTATCAGC ATTTCGGACC GCAGGGCGGC 
CAGGATTCCG GCTTCCCCAG TCCGCGCAGC GCCCTCGGCT ACCCGTTCCC GCCAATGCAC 
CAGAACTCCT ACTCCGGCTA CCATCTGGGC AGCTACGCCC CGCCCTGCGC CAGTCCACCG 
AAGGATGACT TCTCCATCTC CGATAAGTGC GAGGACTCCG GCCTCCGCGT CAACGGCAAG 
GGCAAGAAGA TGCGCAAGCC CCGCACCATC TACAGCTCGC TGCAACTGCA GCAGCTCAAC 
CGCCGCTTCC AGCGTACCCA ATACCTGGCC CTGCCGGAGC GAGCGGAGCT GGCGGCGAGT 
CTGGGCCTCA CGCAAACGCA GGTGAAAATC TGGTTCCAGA ACCGACGCTC CAAGTACAAA 
AAGATGATGA AGGCGGCCCA GGGCCCGGGC ACCAACAGCG GCATGCCTCT GGGCGGGGGA 
GGACCCAATC CCGGCCAGCA TAGCCCCAAT CAAATGCACA GTGGCGGTAA TAACGGCGGT 
GGCTCGAACA GCGGCTCACC CTCACATTAT CTACCTCCCG GACACAGTCC AACGCCATCG 
AGTACACCCG TGTCCGAGCT TTCACCTGAA TTCCCACCAA CGGGCCTCAG TCCGCCGACG 
CAAGCGCCGT GGGACCAGAA ACCGCACTGG ATCGACCACA AGCCGCCGCC CCAAATGACG 
CCACAACCAC CTCATCCAGC GGCAACGCTA CATCCGCAGA CGCACCACCA CAACCCGCCG 
CCCCAGATGG GTGGCTATGT GCCCCAGTAC TGGTACCAGC CCGAGACGAA TCCATCACTA 
GTGACTGTTT GGCCGGCGGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dll ( NM_166689.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726486.1 Drosophila melanogaster Distal-less (Dll), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa11246
Clone ID Related Accession (Same CDS sequence)	NM_166689.2
Accession Version	NM_166689.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Distal-less, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166689.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACGCCC CCGACGCCCC GCACACGCCC AAGTACATGG ACGGCGGGAA CACGGCGGCC 
AGCGTCACGC CCGGCATTAA CATCCCGGGA AAGTCCGCCT TCGTGGAGCT GCAGCAGCAC 
GCCGCCGCCG GGTACGGCGG CATCCGGAGC ACCTATCAGC ATTTCGGACC GCAGGGCGGC 
CAGGATTCCG GCTTCCCCAG TCCGCGCAGC GCCCTCGGCT ACCCGTTCCC GCCAATGCAC 
CAGAACTCCT ACTCCGGCTA CCATCTGGGC AGCTACGCCC CGCCCTGCGC CAGTCCACCG 
AAGGATGACT TCTCCATCTC CGATAAGTGC GAGGACTCCG GCCTCCGCGT CAACGGCAAG 
GGCAAGAAGA TGCGCAAGCC CCGCACCATC TACAGCTCGC TGCAACTGCA GCAGCTCAAC 
CGCCGCTTCC AGCGTACCCA ATACCTGGCC CTGCCGGAGC GAGCGGAGCT GGCGGCGAGT 
CTGGGCCTCA CGCAAACGCA GGTGAAAATC TGGTTCCAGA ACCGACGCTC CAAGTACAAA 
AAGATGATGA AGGCGGCCCA GGGCCCGGGC ACCAACAGCG GCATGCCTCT GGGCGGGGGA 
GGACCCAATC CCGGCCAGCA TAGCCCCAAT CAAATGCACA GTGGCGGTAA TAACGGCGGT 
GGCTCGAACA GCGGCTCACC CTCACATTAT CTACCTCCCG GACACAGTCC AACGCCATCG 
AGTACACCCG TGTCCGAGCT TTCACCTGAA TTCCCACCAA CGGGCCTCAG TCCGCCGACG 
CAAGCGCCGT GGGACCAGAA ACCGCACTGG ATCGACCACA AGCCGCCGCC CCAAATGACG 
CCACAACCAC CTCATCCAGC GGCAACGCTA CATCCGCAGA CGCACCACCA CAACCCGCCG 
CCCCAGATGG GTGGCTATGT GCCCCAGTAC TGGTACCAGC CCGAGACGAA TCCATCACTA 
GTGACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726486.1
CDS	616..1584
Translation	MDAPDAPHTPKYMDGGNTAASVTPGINIPGKSAFVELQQHAAAGYGGIRSTYQHFGPQGGQDSGFPSPRSALGYPFPPMHQNSYSGYHLGSYAPPCASPPKDDFSISDKCEDSGLRVNGKGKKMRKPRTIYSSLQLQQLNRRFQRTQYLALPERAELAASLGLTQTQVKIWFQNRRSKYKKMMKAAQGPGTNSGMPLGGGGPNPGQHSPNQMHSGGNNGGGSNSGSPSHYLPPGHSPTPSSTPVSELSPEFPPTGLSPPTQAPWDQKPHWIDHKPPPQMTPQPPHPAATLHPQTHHHNPPPQMGGYVPQYWYQPETNPSLVT

Target ORF information:

RefSeq Version	NM_166689.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Distal-less (Dll), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166689.2

ORF Insert Sequence:

ATGGACGCCC CCGACGCCCC GCACACGCCC AAGTACATGG ACGGCGGGAA CACGGCGGCC 
AGCGTCACGC CCGGCATTAA CATCCCGGGA AAGTCCGCCT TCGTGGAGCT GCAGCAGCAC 
GCCGCCGCCG GGTACGGCGG CATCCGGAGC ACCTATCAGC ATTTCGGACC GCAGGGCGGC 
CAGGATTCCG GCTTCCCCAG TCCGCGCAGC GCCCTCGGCT ACCCGTTCCC GCCAATGCAC 
CAGAACTCCT ACTCCGGCTA CCATCTGGGC AGCTACGCCC CGCCCTGCGC CAGTCCACCG 
AAGGATGACT TCTCCATCTC CGATAAGTGC GAGGACTCCG GCCTCCGCGT CAACGGCAAG 
GGCAAGAAGA TGCGCAAGCC CCGCACCATC TACAGCTCGC TGCAACTGCA GCAGCTCAAC 
CGCCGCTTCC AGCGTACCCA ATACCTGGCC CTGCCGGAGC GAGCGGAGCT GGCGGCGAGT 
CTGGGCCTCA CGCAAACGCA GGTGAAAATC TGGTTCCAGA ACCGACGCTC CAAGTACAAA 
AAGATGATGA AGGCGGCCCA GGGCCCGGGC ACCAACAGCG GCATGCCTCT GGGCGGGGGA 
GGACCCAATC CCGGCCAGCA TAGCCCCAAT CAAATGCACA GTGGCGGTAA TAACGGCGGT 
GGCTCGAACA GCGGCTCACC CTCACATTAT CTACCTCCCG GACACAGTCC AACGCCATCG 
AGTACACCCG TGTCCGAGCT TTCACCTGAA TTCCCACCAA CGGGCCTCAG TCCGCCGACG 
CAAGCGCCGT GGGACCAGAA ACCGCACTGG ATCGACCACA AGCCGCCGCC CCAAATGACG 
CCACAACCAC CTCATCCAGC GGCAACGCTA CATCCGCAGA CGCACCACCA CAACCCGCCG 
CCCCAGATGG GTGGCTATGT GCCCCAGTAC TGGTACCAGC CCGAGACGAA TCCATCACTA 
GTGACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dll ( NM_001144287.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137759.1 Drosophila melanogaster Distal-less (Dll), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa13250
Clone ID Related Accession (Same CDS sequence)	NM_001144287.2
Accession Version	NM_001144287.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Distal-less, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001144287.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACGCCC CCGACGCCCC GCACACGCCC AAGTACATGG ACGGCGGGAA CACGGCGGCC 
AGCGTCACGC CCGGCATTAA CATCCCGGGA AAGTCCGCCT TCGTGGAGCT GCAGCAGCAC 
GCCGCCGCCG GGTACGGCGG CATCCGGAGC ACCTATCAGC ATTTCGGACC GCAGGGCGGC 
CAGGATTCCG GCTTCCCCAG TCCGCGCAGC GCCCTCGGCT ACCCGTTCCC GCCAATGCAC 
CAGAACTCCT ACTCCGGCTA CCATCTGGGC AGCTACGCCC CGCCCTGCGC CAGTCCACCG 
AAGGATGACT TCTCCATCTC CGATAAGTGC GAGGACTCCG GCCTCCGCGT CAACGGCAAG 
GGCAAGAAGA TGCGCAAGCC CCGCACCATC TACAGCTCGC TGCAACTGCA GCAGCTCAAC 
CGCCGCTTCC AGCGTACCCA ATACCTGGCC CTGCCGGAGC GAGCGGAGCT GGCGGCGAGT 
CTGGGCCTCA CGCAAACGCA GGTGAAAATC TGGTTCCAGA ACCGACGCTC CAAGTACAAA 
AAGATGATGA AGGCGGCCCA GGGCCCGGGC ACCAACAGCG GCATGCCTCT GGGCGGGGGA 
GGACCCAATC CCGGCCAGCA TAGCCCCAAT CAAATGCACA GTGGTGAGTT AGCGAACGGA 
AGATTCCTCT GGGCGGCCTT AGAAACTAAT GGAACTCTCG CTCTTGTCCA TTCCACAGGC 
GGTAATAACG GCGGTGGCTC GAACAGCGGC TCACCCTCAC ATTATCTACC TCCCGGACAC 
AGTCCAACGC CATCGAGTAC ACCCGTGTCC GAGCTTTCAC CTGAATTCCC ACCAACGGGC 
CTCAGTCCGC CGACGCAAGC GCCGTGGGAC CAGAAACCGC ACTGGATCGA CCACAAGCCG 
CCGCCCCAAA TGACGCCACA ACCACCTCAT CCAGCGGCAA CGCTACATCC GCAGACGCAC 
CACCACAACC CGCCGCCCCA GATGGGTGGC TATGTGCCCC AGTACTGGTA CCAGCCCGAG 
ACGAATCCAT CACTAGTGAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137759.1
CDS	616..1659
Translation	MDAPDAPHTPKYMDGGNTAASVTPGINIPGKSAFVELQQHAAAGYGGIRSTYQHFGPQGGQDSGFPSPRSALGYPFPPMHQNSYSGYHLGSYAPPCASPPKDDFSISDKCEDSGLRVNGKGKKMRKPRTIYSSLQLQQLNRRFQRTQYLALPERAELAASLGLTQTQVKIWFQNRRSKYKKMMKAAQGPGTNSGMPLGGGGPNPGQHSPNQMHSGELANGRFLWAALETNGTLALVHSTGGNNGGGSNSGSPSHYLPPGHSPTPSSTPVSELSPEFPPTGLSPPTQAPWDQKPHWIDHKPPPQMTPQPPHPAATLHPQTHHHNPPPQMGGYVPQYWYQPETNPSLVT

Target ORF information:

RefSeq Version	NM_001144287.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Distal-less (Dll), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144287.2

ORF Insert Sequence:

ATGGACGCCC CCGACGCCCC GCACACGCCC AAGTACATGG ACGGCGGGAA CACGGCGGCC 
AGCGTCACGC CCGGCATTAA CATCCCGGGA AAGTCCGCCT TCGTGGAGCT GCAGCAGCAC 
GCCGCCGCCG GGTACGGCGG CATCCGGAGC ACCTATCAGC ATTTCGGACC GCAGGGCGGC 
CAGGATTCCG GCTTCCCCAG TCCGCGCAGC GCCCTCGGCT ACCCGTTCCC GCCAATGCAC 
CAGAACTCCT ACTCCGGCTA CCATCTGGGC AGCTACGCCC CGCCCTGCGC CAGTCCACCG 
AAGGATGACT TCTCCATCTC CGATAAGTGC GAGGACTCCG GCCTCCGCGT CAACGGCAAG 
GGCAAGAAGA TGCGCAAGCC CCGCACCATC TACAGCTCGC TGCAACTGCA GCAGCTCAAC 
CGCCGCTTCC AGCGTACCCA ATACCTGGCC CTGCCGGAGC GAGCGGAGCT GGCGGCGAGT 
CTGGGCCTCA CGCAAACGCA GGTGAAAATC TGGTTCCAGA ACCGACGCTC CAAGTACAAA 
AAGATGATGA AGGCGGCCCA GGGCCCGGGC ACCAACAGCG GCATGCCTCT GGGCGGGGGA 
GGACCCAATC CCGGCCAGCA TAGCCCCAAT CAAATGCACA GTGGTGAGTT AGCGAACGGA 
AGATTCCTCT GGGCGGCCTT AGAAACTAAT GGAACTCTCG CTCTTGTCCA TTCCACAGGC 
GGTAATAACG GCGGTGGCTC GAACAGCGGC TCACCCTCAC ATTATCTACC TCCCGGACAC 
AGTCCAACGC CATCGAGTAC ACCCGTGTCC GAGCTTTCAC CTGAATTCCC ACCAACGGGC 
CTCAGTCCGC CGACGCAAGC GCCGTGGGAC CAGAAACCGC ACTGGATCGA CCACAAGCCG 
CCGCCCCAAA TGACGCCACA ACCACCTCAT CCAGCGGCAA CGCTACATCC GCAGACGCAC 
CACCACAACC CGCCGCCCCA GATGGGTGGC TATGTGCCCC AGTACTGGTA CCAGCCCGAG 
ACGAATCCAT CACTAGTGAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Dll cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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