AANAT1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	AANAT1
Entrez Gene ID	37867
Full Name	Dopamine N acetyltransferase
Synonyms	AA-NAT1,AANAT,AANATA,AANATB,Aanat1,CG3318,DAT,Dat1,Dmel\CG3318,NAT1,aaNAT,aaNAT1,aaNat
General protein information	Preferred Names Dopamine N acetyltransferase Names CG3318 gene product from transcript CG3318-RA CG3318-PA CG3318-PB Dat-PA Dat-PB arylalkylamine N-acetyltransferase arylalkylamine N-acetyltransferase-1 dopamine N acetyltransferase dopamine N-acetyltransferase dopamine acetyltransferase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 60B12-60C1\|2-107 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_206212.2	NP_995934.1	arylalkylamine N-acetyltransferase 1, isoform B
NM_079115.3	NP_523839.2	arylalkylamine N-acetyltransferase 1, isoform A

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Dat	NP_523839.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010142	XP_319299.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Here we report a Drosophila dopamine-related locomotor endophenotype that is shared by pan-neuronal knockdown of orthologs of the ADHD-associated genes Dopamine transporter (DAT1) and Latrophilin (LPHN3), and of a gene causing a monogenic disorder with frequent ADHD comorbidity.
Title: ADHD-associated dopamine transporter, latrophilin and neurofibromin share a dopamine-related locomotor signature in Drosophila.

Dat is entirely responsible for a pigmentation difference between Drosophila species.
Title: A single gene causes an interspecific difference in pigmentation in Drosophila.

Dat possesses a specialized active site structure dedicated to a catalytic mechanism.
Title: Crystal structure of the dopamine N-acetyltransferase-acetyl-CoA complex provides insights into the catalytic mechanism.

Dopamine-dependent arylalkylamine N-acetyltransferase plays a role in the regulation of dopamine content carried out by gonadotropins.
Title: Role of arylalkylamine N-acetyltransferase in regulation of biogenic amines levels by gonadotropins in Drosophila.

The following AANAT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AANAT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa11134	NM_079115.3 Latest version!	Drosophila melanogaster arylalkylamine N-acetyltransferase 1, transcript variant A (AANAT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa11135	NM_206212.2 Latest version!	Drosophila melanogaster arylalkylamine N-acetyltransferase 1, transcript variant B (AANAT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AANAT1 ( NM_079115.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523839.2 Drosophila melanogaster arylalkylamine N-acetyltransferase 1, transcript variant A (AANAT1), mRNA.

Price & Availability↑

CloneID	OFa11134
Clone ID Related Accession (Same CDS sequence)	NM_079115.3
Accession Version	NM_079115.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	arylalkylamine N-acetyltransferase 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_079115.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGACG CATTGACCGT CTCTGGGAAG CCAGCCGCAT GCCCCGTCGA CCAGGACTGC 
CCCTACACCA TCGAACTGAT CCAGCCGGAG GATGGGGAGG CGGTGATAGC CATGCTCAAG 
ACCTTTTTCT TCAAGGATGA ACCGCTGAAC ACCTTCCTCG ACCTTGGCGA GTGCAAGGAG 
CTGGAGAAGT ACTCCCTGAA ACCGCTACCC GACAACTGCT CCTACAAGGC GGTCAACAAG 
AAGGGCGAGA TTATCGGTGT GTTCCTAAAT GGACTTATGA GGCGTCCGTC CCCCGATGAT 
GTGCCCGAAA AGGCGGCCGA CTCCTGTGAA CATCCCAAAT TCAAGAAGAT CCTCTCGCTG 
ATGGACCACG TGGAGGAGCA GTTCAACATC TTCGACGTGT ATCCCGACGA GGAGCTCATC 
CTGGACGGCA AGATCCTGTC GGTGGACACC AACTACCGGG GCCTGGGCAT CGCAGGCCGC 
CTGACGGAGA GGGCGTACGA GTACATGCGG GAGAACGGCA TCAATGTGTA CCACGTGCTC 
TGCTCCAGCC ACTACTCCGC CCGGGTGATG GAGAAGCTGG GCTTCCACGA GGTGTTCCGC 
ATGCAGTTCG CCGACTACAA GCCTCAGGGA GAGGTGGTCT TCAAGCCGGC GGCCCCGCAC 
GTGGGCATAC AGGTGATGGC CAAGGAAGTG GGTCCCGCGA AGGCGGCGCA GACCAAGCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523839.2
CDS	339..1061
Translation	MEDALTVSGKPAACPVDQDCPYTIELIQPEDGEAVIAMLKTFFFKDEPLNTFLDLGECKELEKYSLKPLPDNCSYKAVNKKGEIIGVFLNGLMRRPSPDDVPEKAADSCEHPKFKKILSLMDHVEEQFNIFDVYPDEELILDGKILSVDTNYRGLGIAGRLTERAYEYMRENGINVYHVLCSSHYSARVMEKLGFHEVFRMQFADYKPQGEVVFKPAAPHVGIQVMAKEVGPAKAAQTKL

Target ORF information:

RefSeq Version	NM_079115.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster arylalkylamine N-acetyltransferase 1, transcript variant A (AANAT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079115.3

ORF Insert Sequence:

ATGGAGGACG CATTGACCGT CTCTGGGAAG CCAGCCGCAT GCCCCGTCGA CCAGGACTGC 
CCCTACACCA TCGAACTGAT CCAGCCGGAG GATGGGGAGG CGGTGATAGC CATGCTCAAG 
ACCTTTTTCT TCAAGGATGA ACCGCTGAAC ACCTTCCTCG ACCTTGGCGA GTGCAAGGAG 
CTGGAGAAGT ACTCCCTGAA ACCGCTACCC GACAACTGCT CCTACAAGGC GGTCAACAAG 
AAGGGCGAGA TTATCGGTGT GTTCCTAAAT GGACTTATGA GGCGTCCGTC CCCCGATGAT 
GTGCCCGAAA AGGCGGCCGA CTCCTGTGAA CATCCCAAAT TCAAGAAGAT CCTCTCGCTG 
ATGGACCACG TGGAGGAGCA GTTCAACATC TTCGACGTGT ATCCCGACGA GGAGCTCATC 
CTGGACGGCA AGATCCTGTC GGTGGACACC AACTACCGGG GCCTGGGCAT CGCAGGCCGC 
CTGACGGAGA GGGCGTACGA GTACATGCGG GAGAACGGCA TCAATGTGTA CCACGTGCTC 
TGCTCCAGCC ACTACTCCGC CCGGGTGATG GAGAAGCTGG GCTTCCACGA GGTGTTCCGC 
ATGCAGTTCG CCGACTACAA GCCTCAGGGA GAGGTGGTCT TCAAGCCGGC GGCCCCGCAC 
GTGGGCATAC AGGTGATGGC CAAGGAAGTG GGTCCCGCGA AGGCGGCGCA GACCAAGCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AANAT1 ( NM_206212.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995934.1 Drosophila melanogaster arylalkylamine N-acetyltransferase 1, transcript variant B (AANAT1), mRNA.

Price & Availability↑

CloneID	OFa11135
Clone ID Related Accession (Same CDS sequence)	NM_206212.2
Accession Version	NM_206212.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	arylalkylamine N-acetyltransferase 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_206212.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAGTGC AGAAGCTGCC GGACCAGTCG CTGATATCCA GCATGATGTT GGACTCCCGA 
TGTGGGCTGA ACGACTTGTA TCCGATCGCC CGGCTGACAC AGAAAATGGA GGACGCATTG 
ACCGTCTCTG GGAAGCCAGC CGCATGCCCC GTCGACCAGG ACTGCCCCTA CACCATCGAA 
CTGATCCAGC CGGAGGATGG GGAGGCGGTG ATAGCCATGC TCAAGACCTT TTTCTTCAAG 
GATGAACCGC TGAACACCTT CCTCGACCTT GGCGAGTGCA AGGAGCTGGA GAAGTACTCC 
CTGAAACCGC TACCCGACAA CTGCTCCTAC AAGGCGGTCA ACAAGAAGGG CGAGATTATC 
GGTGTGTTCC TAAATGGACT TATGAGGCGT CCGTCCCCCG ATGATGTGCC CGAAAAGGCG 
GCCGACTCCT GTGAACATCC CAAATTCAAG AAGATCCTCT CGCTGATGGA CCACGTGGAG 
GAGCAGTTCA ACATCTTCGA CGTGTATCCC GACGAGGAGC TCATCCTGGA CGGCAAGATC 
CTGTCGGTGG ACACCAACTA CCGGGGCCTG GGCATCGCAG GCCGCCTGAC GGAGAGGGCG 
TACGAGTACA TGCGGGAGAA CGGCATCAAT GTGTACCACG TGCTCTGCTC CAGCCACTAC 
TCCGCCCGGG TGATGGAGAA GCTGGGCTTC CACGAGGTGT TCCGCATGCA GTTCGCCGAC 
TACAAGCCTC AGGGAGAGGT GGTCTTCAAG CCGGCGGCCC CGCACGTGGG CATACAGGTG 
ATGGCCAAGG AAGTGGGTCC CGCGAAGGCG GCGCAGACCA AGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995934.1
CDS	547..1374
Translation	MEVQKLPDQSLISSMMLDSRCGLNDLYPIARLTQKMEDALTVSGKPAACPVDQDCPYTIELIQPEDGEAVIAMLKTFFFKDEPLNTFLDLGECKELEKYSLKPLPDNCSYKAVNKKGEIIGVFLNGLMRRPSPDDVPEKAADSCEHPKFKKILSLMDHVEEQFNIFDVYPDEELILDGKILSVDTNYRGLGIAGRLTERAYEYMRENGINVYHVLCSSHYSARVMEKLGFHEVFRMQFADYKPQGEVVFKPAAPHVGIQVMAKEVGPAKAAQTKL

Target ORF information:

RefSeq Version	NM_206212.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster arylalkylamine N-acetyltransferase 1, transcript variant B (AANAT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206212.2

ORF Insert Sequence:

ATGGAAGTGC AGAAGCTGCC GGACCAGTCG CTGATATCCA GCATGATGTT GGACTCCCGA 
TGTGGGCTGA ACGACTTGTA TCCGATCGCC CGGCTGACAC AGAAAATGGA GGACGCATTG 
ACCGTCTCTG GGAAGCCAGC CGCATGCCCC GTCGACCAGG ACTGCCCCTA CACCATCGAA 
CTGATCCAGC CGGAGGATGG GGAGGCGGTG ATAGCCATGC TCAAGACCTT TTTCTTCAAG 
GATGAACCGC TGAACACCTT CCTCGACCTT GGCGAGTGCA AGGAGCTGGA GAAGTACTCC 
CTGAAACCGC TACCCGACAA CTGCTCCTAC AAGGCGGTCA ACAAGAAGGG CGAGATTATC 
GGTGTGTTCC TAAATGGACT TATGAGGCGT CCGTCCCCCG ATGATGTGCC CGAAAAGGCG 
GCCGACTCCT GTGAACATCC CAAATTCAAG AAGATCCTCT CGCTGATGGA CCACGTGGAG 
GAGCAGTTCA ACATCTTCGA CGTGTATCCC GACGAGGAGC TCATCCTGGA CGGCAAGATC 
CTGTCGGTGG ACACCAACTA CCGGGGCCTG GGCATCGCAG GCCGCCTGAC GGAGAGGGCG 
TACGAGTACA TGCGGGAGAA CGGCATCAAT GTGTACCACG TGCTCTGCTC CAGCCACTAC 
TCCGCCCGGG TGATGGAGAA GCTGGGCTTC CACGAGGTGT TCCGCATGCA GTTCGCCGAC 
TACAAGCCTC AGGGAGAGGT GGTCTTCAAG CCGGCGGCCC CGCACGTGGG CATACAGGTG 
ATGGCCAAGG AAGTGGGTCC CGCGAAGGCG GCGCAGACCA AGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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AANAT1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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