pita cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	pita
Entrez Gene ID	37730
Full Name	CG3941 gene product from transcript CG3941-RA
Synonyms	CG3941,Dmel\CG3941,PITA,Pita,spdk
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 59E3-59E3

mRNA and Protein(s)

mRNA	Protein	Name
NM_137962.3	NP_611806.3	pita, isoform A
NM_166607.2	NP_726339.2	pita, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	pita	NP_611806.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008845	XP_319587.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mutation of the Mcp Pita site disrupts blocking activity and also eliminates dCTCF binding. In contrast, mutation of the Mcp dCTCF site does not affect Pita binding, and this mutant boundary retains partial function.
Title: Architectural protein Pita cooperates with dCTCF in organization of functional boundaries in Bithorax complex.

Here we have shown that the ZAD domains of ZIPIC, Pita and Zw5 form homodimers. In Drosophila transgenic lines, these proteins are able to support long-distance interaction between GAL4 activator and the reporter gene promoter.
Title: Architectural proteins Pita, Zw5,and ZIPIC contain homodimerization domain and support specific long-range interactions in Drosophila.

Model binding sites for Pita or ZIPIC demonstrate a partial enhancer-blocking activity and protect gene expression from PRE-mediated silencing.
Title: Two new insulator proteins, Pita and ZIPIC, target CP190 to chromatin.

Spotted-dick is a zinc-finger protein of Drosophila required for expression of Orc4 and S phase.
Title: Spotted-dick, a zinc-finger protein of Drosophila required for expression of Orc4 and S phase.

novel characteristic morphological features of egg chambers lacking both dcp-1 and pita functions in the germline cells; suggested an essential role of dcp-1 and/or pita during mid-oogenesis
Title: Morphological irregularities and features of resistance to apoptosis in the dcp-1/pita double mutated egg chambers during Drosophila oogenesis.

The following pita gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pita cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa11054	NM_166607.2 Latest version!	Drosophila melanogaster pita, transcript variant B (pita), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa11053	NM_137962.3 Latest version!	Drosophila melanogaster pita, transcript variant A (pita), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pita ( NM_166607.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726339.2 Drosophila melanogaster pita, transcript variant B (pita), mRNA.

Price & Availability↑

CloneID	OFa11054
Clone ID Related Accession (Same CDS sequence)	NM_166607.2
Accession Version	NM_166607.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pita, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166607.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGGGGC ACATCTGTCT GGAGTGCCGC CTCCTCTTCG AGCACTGCTA CCGCTTCAAG 
CAGATGTGCA AGCGGGCGGA GACCCTCCTG CGCCAGTATC CGCTCACGGG TAACTGGCCA 
TCGCCGCTGG AGAAGCCCCG TGCGCCCATG ACGATGGTGG CTTCCAAGAA GTTGTTGGTC 
GTGCCAGCAA AGACAGCCGA GCCAAGCGAA ACGCCCAAAA AGCTGCTGAA CACAATGGCC 
AAATCCAGTA GTCAGGTGAT CATCGAGGAC GTCCAGGTGC TGGAGAGCGC CATGGTGACC 
CCCAGGACGG TAGCCGGTTC CTCTCCAGTG CCACGTCGTT CGCACGCCTA TGAGCTGAAG 
GTGGATAACA ACCAGGAGCT GTCCATGGAC GATGTGCAGA GCATGCTGGA GGATATGGCC 
AGTGAGCTAG AGAAGGAGTT CCCCGATATC CCCCAGAAGG CATCACCCGT CAAGCCGAAG 
GTGCTGAACA AGTCCTCGAT TAGGATCTTG AACAAGGGAC CCGCCGCTCC GGTGGAACCG 
CGCCTGGCCA CACCCAAAGT CAAGCGAGAT GATAGTGGCA ATGTGGCCAT AGTGACAGAG 
GTTCTGGACT CGGATCTGCC ATTGGACGAT CAAGATGATC CGACCAAGAA CGCCGAGAAG 
GTGGCCACCG ATGTGTTCCC CTGTCCCGAT TGCGAACGCT CCTTTCCGCT GCAGCAGCTG 
CTCGAGATCC ATCGGCTGAA TCACACTCGC TCGCGCAGCT TCCAGTGTCT CCTGTGCGAG 
AAGAGCTTCT TCTCCAAGTA CGACCTGGCC AAGCACAACT TTGTGCACAC CGGCGAGCGG 
CCATTCAAGT GCGCCATCTG CTCGAAAGCC TTCACACGGA AGGCATTGCT CCATCGCCAC 
GAGCGCACCC ACACGGACGT ACCCAAGTTC ATCTGCGTGT ACTGCGAGAA GCCCTTCCTT 
TCGCGCCAGG AGATGGAGAA GCACGCCGAG CGACATCAGA AGAAGCGTCC GTTCCAATGC 
GGTGTCTGCA CCAAGTCGTT TGCCTTCAAG CAGGGATTGG AGCGTCATGA GACAGTCCAC 
TCCACCAATC TTCCCTTCCC CTGTCAGCAT TGCGAGAGAA GCTTTTCCAC CGCTTCCAAG 
CTGGCCCGCC ACCTGGTTGC CCACGCCGGG AAGAGAGCCT ATCCCTGCAA GTACTGCCAC 
AAGTCCTACA TGCTGTCGCA TCATCTCTCG CGCCACCTGC GCACGCACAC GCAGACTTCC 
GACGCCTCGT TCGTGTGCAG TGAGTGCAAG GTGTCGTACA GCAACTATAA CGACCTACTG 
GATCATGCGC TCATTCATGC CACCGCCAGC CTGAAATGTC CCATGTGTCG CAAGCAGATC 
GAAGATATCG ACAGTGTCGA GAGCCACATG GACCAGCACA AGCAGAGCGA GCGCCATGCC 
TGCGAGTTCT GTGACCACAT CTTCTTGACG CAGAAATGCC TGCAGCGCCA CATCGAGGAC 
GATCATGTGG TGGAAATGGA GCCGTACCAG AACGAGTTCG AAGACGACGG CGAAGGAGAA 
GGCGGCGTCG ATGAGAAGGA GGAACATCTT GACGATTTTG AAGACATGGA CAATGTGAAG 
CAGGAAGAAT TCGTGACGGA ATACTTAGAA GACGATGCTC TCTATGAGGA CCACCTAGAC 
GATAGCGACG AATCCTTCAC GCCGCCGCCT CCAAAGCAGC GTAAAACCAA TCCTCCCAAG 
GATGCCCAGC AATCCGTGCG CCAAACTCGC AGTCGGGATG CTCAAAGAAT CACTCAGAAG 
ACAGGAAAGA ACGAAGGCAA GCCTCATCAC AAACTGGAAC GCAACCTTAA GAATCGCCGA 
TCCGCAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726339.2
CDS	534..2405
Translation	MPGHICLECRLLFEHCYRFKQMCKRAETLLRQYPLTGNWPSPLEKPRAPMTMVASKKLLVVPAKTAEPSETPKKLLNTMAKSSSQVIIEDVQVLESAMVTPRTVAGSSPVPRRSHAYELKVDNNQELSMDDVQSMLEDMASELEKEFPDIPQKASPVKPKVLNKSSIRILNKGPAAPVEPRLATPKVKRDDSGNVAIVTEVLDSDLPLDDQDDPTKNAEKVATDVFPCPDCERSFPLQQLLEIHRLNHTRSRSFQCLLCEKSFFSKYDLAKHNFVHTGERPFKCAICSKAFTRKALLHRHERTHTDVPKFICVYCEKPFLSRQEMEKHAERHQKKRPFQCGVCTKSFAFKQGLERHETVHSTNLPFPCQHCERSFSTASKLARHLVAHAGKRAYPCKYCHKSYMLSHHLSRHLRTHTQTSDASFVCSECKVSYSNYNDLLDHALIHATASLKCPMCRKQIEDIDSVESHMDQHKQSERHACEFCDHIFLTQKCLQRHIEDDHVVEMEPYQNEFEDDGEGEGGVDEKEEHLDDFEDMDNVKQEEFVTEYLEDDALYEDHLDDSDESFTPPPPKQRKTNPPKDAQQSVRQTRSRDAQRITQKTGKNEGKPHHKLERNLKNRRSAK

Target ORF information:

RefSeq Version	NM_166607.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pita, transcript variant B (pita), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166607.2

ORF Insert Sequence:

ATGCCGGGGC ACATCTGTCT GGAGTGCCGC CTCCTCTTCG AGCACTGCTA CCGCTTCAAG 
CAGATGTGCA AGCGGGCGGA GACCCTCCTG CGCCAGTATC CGCTCACGGG TAACTGGCCA 
TCGCCGCTGG AGAAGCCCCG TGCGCCCATG ACGATGGTGG CTTCCAAGAA GTTGTTGGTC 
GTGCCAGCAA AGACAGCCGA GCCAAGCGAA ACGCCCAAAA AGCTGCTGAA CACAATGGCC 
AAATCCAGTA GTCAGGTGAT CATCGAGGAC GTCCAGGTGC TGGAGAGCGC CATGGTGACC 
CCCAGGACGG TAGCCGGTTC CTCTCCAGTG CCACGTCGTT CGCACGCCTA TGAGCTGAAG 
GTGGATAACA ACCAGGAGCT GTCCATGGAC GATGTGCAGA GCATGCTGGA GGATATGGCC 
AGTGAGCTAG AGAAGGAGTT CCCCGATATC CCCCAGAAGG CATCACCCGT CAAGCCGAAG 
GTGCTGAACA AGTCCTCGAT TAGGATCTTG AACAAGGGAC CCGCCGCTCC GGTGGAACCG 
CGCCTGGCCA CACCCAAAGT CAAGCGAGAT GATAGTGGCA ATGTGGCCAT AGTGACAGAG 
GTTCTGGACT CGGATCTGCC ATTGGACGAT CAAGATGATC CGACCAAGAA CGCCGAGAAG 
GTGGCCACCG ATGTGTTCCC CTGTCCCGAT TGCGAACGCT CCTTTCCGCT GCAGCAGCTG 
CTCGAGATCC ATCGGCTGAA TCACACTCGC TCGCGCAGCT TCCAGTGTCT CCTGTGCGAG 
AAGAGCTTCT TCTCCAAGTA CGACCTGGCC AAGCACAACT TTGTGCACAC CGGCGAGCGG 
CCATTCAAGT GCGCCATCTG CTCGAAAGCC TTCACACGGA AGGCATTGCT CCATCGCCAC 
GAGCGCACCC ACACGGACGT ACCCAAGTTC ATCTGCGTGT ACTGCGAGAA GCCCTTCCTT 
TCGCGCCAGG AGATGGAGAA GCACGCCGAG CGACATCAGA AGAAGCGTCC GTTCCAATGC 
GGTGTCTGCA CCAAGTCGTT TGCCTTCAAG CAGGGATTGG AGCGTCATGA GACAGTCCAC 
TCCACCAATC TTCCCTTCCC CTGTCAGCAT TGCGAGAGAA GCTTTTCCAC CGCTTCCAAG 
CTGGCCCGCC ACCTGGTTGC CCACGCCGGG AAGAGAGCCT ATCCCTGCAA GTACTGCCAC 
AAGTCCTACA TGCTGTCGCA TCATCTCTCG CGCCACCTGC GCACGCACAC GCAGACTTCC 
GACGCCTCGT TCGTGTGCAG TGAGTGCAAG GTGTCGTACA GCAACTATAA CGACCTACTG 
GATCATGCGC TCATTCATGC CACCGCCAGC CTGAAATGTC CCATGTGTCG CAAGCAGATC 
GAAGATATCG ACAGTGTCGA GAGCCACATG GACCAGCACA AGCAGAGCGA GCGCCATGCC 
TGCGAGTTCT GTGACCACAT CTTCTTGACG CAGAAATGCC TGCAGCGCCA CATCGAGGAC 
GATCATGTGG TGGAAATGGA GCCGTACCAG AACGAGTTCG AAGACGACGG CGAAGGAGAA 
GGCGGCGTCG ATGAGAAGGA GGAACATCTT GACGATTTTG AAGACATGGA CAATGTGAAG 
CAGGAAGAAT TCGTGACGGA ATACTTAGAA GACGATGCTC TCTATGAGGA CCACCTAGAC 
GATAGCGACG AATCCTTCAC GCCGCCGCCT CCAAAGCAGC GTAAAACCAA TCCTCCCAAG 
GATGCCCAGC AATCCGTGCG CCAAACTCGC AGTCGGGATG CTCAAAGAAT CACTCAGAAG 
ACAGGAAAGA ACGAAGGCAA GCCTCATCAC AAACTGGAAC GCAACCTTAA GAATCGCCGA 
TCCGCAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pita ( NM_137962.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611806.3 Drosophila melanogaster pita, transcript variant A (pita), mRNA.

Price & Availability↑

CloneID	OFa11053
Clone ID Related Accession (Same CDS sequence)	NM_137962.3
Accession Version	NM_137962.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pita, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137962.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGCGA AACTGGAGGT GCGCGAGGCC ATGCTGACCG AGAAGAGGGT CTGCCGCTTC 
TGTCTGACCG AGCAGAAGCT GGCCTCGATT TTCGAGGAGA ATCCGCGGGT GAAAACAACC 
GCCAATCTGC CGCTGCAGAT TATGGCCATC ACGGCGATCG AGGTCTACGC CGGCGACGGA 
ATGCCGGGGC ACATCTGTCT GGAGTGCCGC CTCCTCTTCG AGCACTGCTA CCGCTTCAAG 
CAGATGTGCA AGCGGGCGGA GACCCTCCTG CGCCAGTATC CGCTCACGGG TAACTGGCCA 
TCGCCGCTGG AGAAGCCCCG TGCGCCCATG ACGATGGTGG CTTCCAAGAA GTTGTTGGTC 
GTGCCAGCAA AGACAGCCGA GCCAAGCGAA ACGCCCAAAA AGCTGCTGAA CACAATGGCC 
AAATCCAGTA GTCAGGTGAT CATCGAGGAC GTCCAGGTGC TGGAGAGCGC CATGGTGACC 
CCCAGGACGG TAGCCGGTTC CTCTCCAGTG CCACGTCGTT CGCACGCCTA TGAGCTGAAG 
GTGGATAACA ACCAGGAGCT GTCCATGGAC GATGTGCAGA GCATGCTGGA GGATATGGCC 
AGTGAGCTAG AGAAGGAGTT CCCCGATATC CCCCAGAAGG CATCACCCGT CAAGCCGAAG 
GTGCTGAACA AGTCCTCGAT TAGGATCTTG AACAAGGGAC CCGCCGCTCC GGTGGAACCG 
CGCCTGGCCA CACCCAAAGT CAAGCGAGAT GATAGTGGCA ATGTGGCCAT AGTGACAGAG 
GTTCTGGACT CGGATCTGCC ATTGGACGAT CAAGATGATC CGACCAAGAA CGCCGAGAAG 
GTGGCCACCG ATGTGTTCCC CTGTCCCGAT TGCGAACGCT CCTTTCCGCT GCAGCAGCTG 
CTCGAGATCC ATCGGCTGAA TCACACTCGC TCGCGCAGCT TCCAGTGTCT CCTGTGCGAG 
AAGAGCTTCT TCTCCAAGTA CGACCTGGCC AAGCACAACT TTGTGCACAC CGGCGAGCGG 
CCATTCAAGT GCGCCATCTG CTCGAAAGCC TTCACACGGA AGGCATTGCT CCATCGCCAC 
GAGCGCACCC ACACGGACGT ACCCAAGTTC ATCTGCGTGT ACTGCGAGAA GCCCTTCCTT 
TCGCGCCAGG AGATGGAGAA GCACGCCGAG CGACATCAGA AGAAGCGTCC GTTCCAATGC 
GGTGTCTGCA CCAAGTCGTT TGCCTTCAAG CAGGGATTGG AGCGTCATGA GACAGTCCAC 
TCCACCAATC TTCCCTTCCC CTGTCAGCAT TGCGAGAGAA GCTTTTCCAC CGCTTCCAAG 
CTGGCCCGCC ACCTGGTTGC CCACGCCGGG AAGAGAGCCT ATCCCTGCAA GTACTGCCAC 
AAGTCCTACA TGCTGTCGCA TCATCTCTCG CGCCACCTGC GCACGCACAC GCAGACTTCC 
GACGCCTCGT TCGTGTGCAG TGAGTGCAAG GTGTCGTACA GCAACTATAA CGACCTACTG 
GATCATGCGC TCATTCATGC CACCGCCAGC CTGAAATGTC CCATGTGTCG CAAGCAGATC 
GAAGATATCG ACAGTGTCGA GAGCCACATG GACCAGCACA AGCAGAGCGA GCGCCATGCC 
TGCGAGTTCT GTGACCACAT CTTCTTGACG CAGAAATGCC TGCAGCGCCA CATCGAGGAC 
GATCATGTGG TGGAAATGGA GCCGTACCAG AACGAGTTCG AAGACGACGG CGAAGGAGAA 
GGCGGCGTCG ATGAGAAGGA GGAACATCTT GACGATTTTG AAGACATGGA CAATGTGAAG 
CAGGAAGAAT TCGTGACGGA ATACTTAGAA GACGATGCTC TCTATGAGGA CCACCTAGAC 
GATAGCGACG AATCCTTCAC GCCGCCGCCT CCAAAGCAGC GTAAAACCAA TCCTCCCAAG 
GATGCCCAGC AATCCGTGCG CCAAACTCGC AGTCGGGATG CTCAAAGAAT CACTCAGAAG 
ACAGGAAAGA ACGAAGGCAA GCCTCATCAC AAACTGGAAC GCAACCTTAA GAATCGCCGA 
TCCGCAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611806.3
CDS	297..2348
Translation	MAAKLEVREAMLTEKRVCRFCLTEQKLASIFEENPRVKTTANLPLQIMAITAIEVYAGDGMPGHICLECRLLFEHCYRFKQMCKRAETLLRQYPLTGNWPSPLEKPRAPMTMVASKKLLVVPAKTAEPSETPKKLLNTMAKSSSQVIIEDVQVLESAMVTPRTVAGSSPVPRRSHAYELKVDNNQELSMDDVQSMLEDMASELEKEFPDIPQKASPVKPKVLNKSSIRILNKGPAAPVEPRLATPKVKRDDSGNVAIVTEVLDSDLPLDDQDDPTKNAEKVATDVFPCPDCERSFPLQQLLEIHRLNHTRSRSFQCLLCEKSFFSKYDLAKHNFVHTGERPFKCAICSKAFTRKALLHRHERTHTDVPKFICVYCEKPFLSRQEMEKHAERHQKKRPFQCGVCTKSFAFKQGLERHETVHSTNLPFPCQHCERSFSTASKLARHLVAHAGKRAYPCKYCHKSYMLSHHLSRHLRTHTQTSDASFVCSECKVSYSNYNDLLDHALIHATASLKCPMCRKQIEDIDSVESHMDQHKQSERHACEFCDHIFLTQKCLQRHIEDDHVVEMEPYQNEFEDDGEGEGGVDEKEEHLDDFEDMDNVKQEEFVTEYLEDDALYEDHLDDSDESFTPPPPKQRKTNPPKDAQQSVRQTRSRDAQRITQKTGKNEGKPHHKLERNLKNRRSAK

Target ORF information:

RefSeq Version	NM_137962.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pita, transcript variant A (pita), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137962.3

ORF Insert Sequence:

ATGGCCGCGA AACTGGAGGT GCGCGAGGCC ATGCTGACCG AGAAGAGGGT CTGCCGCTTC 
TGTCTGACCG AGCAGAAGCT GGCCTCGATT TTCGAGGAGA ATCCGCGGGT GAAAACAACC 
GCCAATCTGC CGCTGCAGAT TATGGCCATC ACGGCGATCG AGGTCTACGC CGGCGACGGA 
ATGCCGGGGC ACATCTGTCT GGAGTGCCGC CTCCTCTTCG AGCACTGCTA CCGCTTCAAG 
CAGATGTGCA AGCGGGCGGA GACCCTCCTG CGCCAGTATC CGCTCACGGG TAACTGGCCA 
TCGCCGCTGG AGAAGCCCCG TGCGCCCATG ACGATGGTGG CTTCCAAGAA GTTGTTGGTC 
GTGCCAGCAA AGACAGCCGA GCCAAGCGAA ACGCCCAAAA AGCTGCTGAA CACAATGGCC 
AAATCCAGTA GTCAGGTGAT CATCGAGGAC GTCCAGGTGC TGGAGAGCGC CATGGTGACC 
CCCAGGACGG TAGCCGGTTC CTCTCCAGTG CCACGTCGTT CGCACGCCTA TGAGCTGAAG 
GTGGATAACA ACCAGGAGCT GTCCATGGAC GATGTGCAGA GCATGCTGGA GGATATGGCC 
AGTGAGCTAG AGAAGGAGTT CCCCGATATC CCCCAGAAGG CATCACCCGT CAAGCCGAAG 
GTGCTGAACA AGTCCTCGAT TAGGATCTTG AACAAGGGAC CCGCCGCTCC GGTGGAACCG 
CGCCTGGCCA CACCCAAAGT CAAGCGAGAT GATAGTGGCA ATGTGGCCAT AGTGACAGAG 
GTTCTGGACT CGGATCTGCC ATTGGACGAT CAAGATGATC CGACCAAGAA CGCCGAGAAG 
GTGGCCACCG ATGTGTTCCC CTGTCCCGAT TGCGAACGCT CCTTTCCGCT GCAGCAGCTG 
CTCGAGATCC ATCGGCTGAA TCACACTCGC TCGCGCAGCT TCCAGTGTCT CCTGTGCGAG 
AAGAGCTTCT TCTCCAAGTA CGACCTGGCC AAGCACAACT TTGTGCACAC CGGCGAGCGG 
CCATTCAAGT GCGCCATCTG CTCGAAAGCC TTCACACGGA AGGCATTGCT CCATCGCCAC 
GAGCGCACCC ACACGGACGT ACCCAAGTTC ATCTGCGTGT ACTGCGAGAA GCCCTTCCTT 
TCGCGCCAGG AGATGGAGAA GCACGCCGAG CGACATCAGA AGAAGCGTCC GTTCCAATGC 
GGTGTCTGCA CCAAGTCGTT TGCCTTCAAG CAGGGATTGG AGCGTCATGA GACAGTCCAC 
TCCACCAATC TTCCCTTCCC CTGTCAGCAT TGCGAGAGAA GCTTTTCCAC CGCTTCCAAG 
CTGGCCCGCC ACCTGGTTGC CCACGCCGGG AAGAGAGCCT ATCCCTGCAA GTACTGCCAC 
AAGTCCTACA TGCTGTCGCA TCATCTCTCG CGCCACCTGC GCACGCACAC GCAGACTTCC 
GACGCCTCGT TCGTGTGCAG TGAGTGCAAG GTGTCGTACA GCAACTATAA CGACCTACTG 
GATCATGCGC TCATTCATGC CACCGCCAGC CTGAAATGTC CCATGTGTCG CAAGCAGATC 
GAAGATATCG ACAGTGTCGA GAGCCACATG GACCAGCACA AGCAGAGCGA GCGCCATGCC 
TGCGAGTTCT GTGACCACAT CTTCTTGACG CAGAAATGCC TGCAGCGCCA CATCGAGGAC 
GATCATGTGG TGGAAATGGA GCCGTACCAG AACGAGTTCG AAGACGACGG CGAAGGAGAA 
GGCGGCGTCG ATGAGAAGGA GGAACATCTT GACGATTTTG AAGACATGGA CAATGTGAAG 
CAGGAAGAAT TCGTGACGGA ATACTTAGAA GACGATGCTC TCTATGAGGA CCACCTAGAC 
GATAGCGACG AATCCTTCAC GCCGCCGCCT CCAAAGCAGC GTAAAACCAA TCCTCCCAAG 
GATGCCCAGC AATCCGTGCG CCAAACTCGC AGTCGGGATG CTCAAAGAAT CACTCAGAAG 
ACAGGAAAGA ACGAAGGCAA GCCTCATCAC AAACTGGAAC GCAACCTTAA GAATCGCCGA 
TCCGCAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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pita cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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