mei-218 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	mei-218
Entrez Gene ID	3772646
Full Name	meiotic 218
Synonyms	CG33067,CG8923,Dmel\CG8923
General protein information	Preferred Names meiotic 218 Names CG8923 gene product from transcript CG8923-RD CG8923-PB CG8923-PC CG8923-PD CG8923-PE mei-218-PB mei-218-PC mei-218-PD mei-218-PE transcript c
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 15E5-15E5

mRNA and Protein(s)

mRNA	Protein	Name
NM_001031897.3	NP_001027068.1	meiotic 218, isoform C
NM_001144743.2	NP_001138215.1	meiotic 218, isoform D
NM_167557.3	NP_728052.2	meiotic 218, isoform B
NM_001201728.1	NP_001188657.1	meiotic 218, isoform E

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Intragenic recombination experiments suggest that mei-218 is required for a molecular transition of the recombination intermediate late in the double strand break repair pathway
Title: Studies on crossover-specific mutants and the distribution of crossing over in Drosophila females.

MEI-218 is a protein required for meiotic crossing-over in Drosophila.
Title: Cytoplasmic localization and evolutionary conservation of MEI-218, a protein required for meiotic crossing-over in Drosophila.

The following mei-218 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mei-218 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa02609	NM_001031897.3 Latest version!	Drosophila melanogaster meiotic 218 (mei-218), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFa02609	NM_001201728.1 Latest version!	Drosophila melanogaster meiotic 218 (mei-218), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFa02609	NM_001144743.2 Latest version!	Drosophila melanogaster meiotic 218 (mei-218), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFa02609	NM_167557.3 Latest version!	Drosophila melanogaster meiotic 218 (mei-218), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mei-218 ( NM_001031897.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001027068.1 Drosophila melanogaster meiotic 218 (mei-218), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa02609
Clone ID Related Accession (Same CDS sequence)	NM_001031897.3 , NM_001144743.2 , NM_167557.3 , NM_001201728.1
Accession Version	NM_001031897.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	meiotic 218, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 31, 2011 this sequence version replaced NM_001031897.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCATCTA CGAATAAACT AGAGAAAAAG GACCCTCGTA GGCACATTCC CCTCCAGCTT 
AATGTTTTCT TGCGCAACGA TGACGATGGT GAAGTTCGTG AAGTTACTCA AAATGCCAAC 
ACAAGCTCCA TAGGCACCAT ACCATCGAAT GGCCTGGTCG AAAGCGATGT GACGCCTATT 
ATGACTCCCA CACCGAAGGC CGGTGTCTAC AAACCAAATG TTAACGTCTG GCGCTTATCT 
AAAGAAGTCA CTAAGCCTGA TCGTAGCTTT GTTGTGAGTG CCGAAGAGTA TCCTGATTTG 
GGCCAGCGCC CGAAGAGATT CAAACAACGA ACGCCGCGGG TGCGTAATCA AGATGACGGC 
GGCACTGTTT CGTGCCTTGC CAGTCGTTTT AGTCGCGTAA AAGATCCGCC TGTGCTGGGT 
AACTTTTTGC CGGATTTCAT GCTTCCGAAA TCGAGCAACA GCAATCATCA ATCGGTGACC 
ACTTGGCTGT CGTCAGTACG TCCTAGCGAC AGCCAAAAGT CTTTAAGCGA CCAGGCACGT 
TGCTCTTCTT CGACCAATAT AACACTGCTG AGTAAAGATT CAGTACAGCC GCTGACGCCA 
AAAAGTTCTG CCCAGTCCTC TCCGGGTGAG AAGAATATCC AACAGGAGCA GAATACTCCA 
GCTAAATCCC AACAGGAGGA GACAAGTCCG GCTAATTCTG TCGAGCATCA GCAGAAAACA 
CCTAACTCTC CAAAGGAGCA GCAGAAGAGT TCTGTCGGTT TTACTAACAT GCAGCAGATG 
GCTTCCGCCA ATTCTTTACA GCAGCAAAAC AGTTCTGCCA GTTCCTTACA GCAGCAGAAT 
AGTTCGGTCC ATTCCTTACA GAAGCGGAAG AGTTGGGTCA ATTCTTCACA GCAGCAGAGG 
AGTTCGGTCA ATTTCATACA GAAGCAAAGG AGTTGGGTCA ATTCCTCGCA TCATCAGAAG 
AGTTCTGCCA GTTCTTTACA GCAGAAGAGT TCTGAAAATT CTCCCAGCAA ATTCCAACAG 
CAGCAACAGA AGAGTCCTGC CAATAATGAA AGGAGCCAAA ACAATCAGAA GTCTCCTGGC 
ATTCAGCACT CTTGGAAACA ACAACAGCAG CAGAAGCCAA AGATTCTTCA GAATTGTCCG 
AAAACTTCTG TTCAGCAGCA GATGAACCCT GTTAATCATA CTCAGTCGCA ATCAGAGAGA 
CCTACCGAGC TCATTTACTT GCACTCAAGG AGCCCTGCGA AGAGAGCTTC AGTTACAAAC 
AATTCTTCGG CACCAATCCG CATTGCGCCC AGACAGCAGA CCACTCAATC TTCGGGTCCC 
AGTGTTGTCC ACAATGAGGA CGAGGATGTT ATGCCGCCGA CGCCTAATCA AAGTGAGAGA 
AGGCAGAGGA GATTCCCACG TCGCAATCAC AACCCCCGGA TGGTGCGCAG TCTGCAGGCG 
GTAAGTCGAG AGTTTACAGA GTCCTTGCTT GAGGGATCTT CAGGGAAAAC GCCGGAAAAA 
GAGGTGGTGC CGTACCAGCA ACAGGAGAAA CCGCGCCAGG TGAAAAGCTC ACCAGAAATC 
CATGCGAGCA GCCACATGCG AAAATTGGCT ATGGGCAGCG TTTTGAACTC AAATGCTAAG 
GAGTTCCACC CTACCGGAGA ATCCCATCTA ACCCACAAAG TGTCTAGATT GAGCCAAAGA 
CGCTGTCGAC AGCGTTTTAC GCGCTCTTTG CACGCGAAAA AAAATAACCC ACGCGAGCAA 
CTACCAAATC CTGACAGCGC CCCAGAACCA ATACTCGAAT ACACCTCTGA GATAGATGAT 
CAGCCATGTC CACCACGACA GACAGTCGAC ACACCGATCG AAGCCATTGT ACCACCGGCA 
ATGATCGAGG TGAGTGCCAC CGGCAATCTG CCGCCCGTGT TCTATTTTGA TCAGCAGTCG 
GGAATTATAT GGAGCAACTC GTCACCACAA ATTCCGGTTG AAATCCAAAG CAACAGTGTC 
AGCCAGTCGG TTTTTCGGCT AGCCAGTCTC CCGTGTACAG GGGTTGGCAC CACCAATATG 
GATATATTTA CCAGTTCGCC ACCCCCCATC CAGCATATGA GCTCCAGCCG CCTCCCATCC 
AGCATTTCCG GGCCCACCCA GTTATCGCAT ACTCTCAGTT CGCCGCCGGT GGAGAACCAG 
CACCAGCAAC ACTTGTGGGA ATCATGCATC CACCAGGACG AGGACCTAAT GTACAGCAGG 
CCTCTGTTGG ATATGGATCA GGAGGAAACC CAGCCGGAGC AACAGGACCA GGAATGGCAG 
TGGGAGTCGG AGATAGAGCC CCCGAGTTTC CAGTCGGAGA ACCAGGTCGT CGCCGACGAA 
CACGGCGAGG AAAACGCGTA CCTCGCTAAG GACGCAGGAC CACCACTCGA GCGTCATATG 
ATATTTCGAG CCCCATCACA GCACGGCCAA GAATATCCCA AGCCGGATCT AAACTGCGTG 
CCGTCCAGCA TCAAGAAACT ACATCACTTG ATCTCATCGC AGTATTCTGA CTACTCCTTT 
GTCTACGCCC TAAGTGCTCA GATCAGTCAG GACTGTGTGC CTATGGATTG CTTTGTGTAC 
CTGAAGATGA TACTGCTGGC CAGTATCGTT TCAATCGAAT CTGATGAGGT GCGGGCTCCA 
ATTTCGCTAT GCATAATCGC CACCGACAGC CTGATGGCCA ACCGTCTACT TAACAAAGTG 
GGCCAACTGG CACCACGTTT CCTGGGACCC CACGAATACG GACTGCAGCC CACTTTCAAT 
GCCCTGCCCA CGCGCTTTAA TTGGATAGTG GCCAGTCCTT TACTATTGGC ACAACAGGGA 
GTTTACTACG CCGGCGATTG GAATCGTCTG TCCAAGGATC AGGGATGCCA GCTGGAAAAG 
TGCATCGAAA ATGGAGCGGT CCCAGTTCCG CAGTTGCACA TCGATCAGCC CTTAAAGGCA 
GCCGTTTGGA CTTACTGGCA GCCGAACAAC TCCACCAATC AAACACTGGC CCTCGCCAAA 
CTGTGCCCAA TCTTCGGGCT GCCAATTTAT ATGGGTGCTC AGGCCAGCAA TAGTCTCTGG 
AACTCTATAA TTCAGAAGCA CAACGCTGAG GGTCAAATTG TAGTCAACGA TGGCTTGAGC 
ATTCCCGAGG ACGATATGCG CATGCTTATA CATCTGTTGC ATCAGCGCAA GACCACCTTA 
ACCGATGGCG CTCAGCATAT GCTGCAAAAG TATTATGTGA TCTCCAGGAA AGAGCGACCG 
AATGTCTTCT CTAGCAAGAC CTATATAGTG CTGAAGCAAT TAGCTGAGTG CTTTGCCAAG 
CTGGCGCTGC GGCTGGAGGT CCTTGAATCG GACGTTTGTG TGGCCATCTT TCACTGCGAG 
CATTTCGTGC AGGGCATTTT CGGGGCCAAA GAAAACCAGG CACCGCCGGC GGTCTTCAAT 
TTCAATGTGA TCTCCTGCAT CGATCCGTAC ATGAACAAGT TTACACGCTG GTTACTTCAG 
TATCTGAATA GCTACGAGGA CGAGGAGCTG GGGATGCACG CGGATAAGAG GCGGCGCACA 
GATAGCTGGG ACATGCCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001027068.1
CDS	946..4506
Translation	MSSTNKLEKKDPRRHIPLQLNVFLRNDDDGEVREVTQNANTSSIGTIPSNGLVESDVTPIMTPTPKAGVYKPNVNVWRLSKEVTKPDRSFVVSAEEYPDLGQRPKRFKQRTPRVRNQDDGGTVSCLASRFSRVKDPPVLGNFLPDFMLPKSSNSNHQSVTTWLSSVRPSDSQKSLSDQARCSSSTNITLLSKDSVQPLTPKSSAQSSPGEKNIQQEQNTPAKSQQEETSPANSVEHQQKTPNSPKEQQKSSVGFTNMQQMASANSLQQQNSSASSLQQQNSSVHSLQKRKSWVNSSQQQRSSVNFIQKQRSWVNSSHHQKSSASSLQQKSSENSPSKFQQQQQKSPANNERSQNNQKSPGIQHSWKQQQQQKPKILQNCPKTSVQQQMNPVNHTQSQSERPTELIYLHSRSPAKRASVTNNSSAPIRIAPRQQTTQSSGPSVVHNEDEDVMPPTPNQSERRQRRFPRRNHNPRMVRSLQAVSREFTESLLEGSSGKTPEKEVVPYQQQEKPRQVKSSPEIHASSHMRKLAMGSVLNSNAKEFHPTGESHLTHKVSRLSQRRCRQRFTRSLHAKKNNPREQLPNPDSAPEPILEYTSEIDDQPCPPRQTVDTPIEAIVPPAMIEVSATGNLPPVFYFDQQSGIIWSNSSPQIPVEIQSNSVSQSVFRLASLPCTGVGTTNMDIFTSSPPPIQHMSSSRLPSSISGPTQLSHTLSSPPVENQHQQHLWESCIHQDEDLMYSRPLLDMDQEETQPEQQDQEWQWESEIEPPSFQSENQVVADEHGEENAYLAKDAGPPLERHMIFRAPSQHGQEYPKPDLNCVPSSIKKLHHLISSQYSDYSFVYALSAQISQDCVPMDCFVYLKMILLASIVSIESDEVRAPISLCIIATDSLMANRLLNKVGQLAPRFLGPHEYGLQPTFNALPTRFNWIVASPLLLAQQGVYYAGDWNRLSKDQGCQLEKCIENGAVPVPQLHIDQPLKAAVWTYWQPNNSTNQTLALAKLCPIFGLPIYMGAQASNSLWNSIIQKHNAEGQIVVNDGLSIPEDDMRMLIHLLHQRKTTLTDGAQHMLQKYYVISRKERPNVFSSKTYIVLKQLAECFAKLALRLEVLESDVCVAIFHCEHFVQGIFGAKENQAPPAVFNFNVISCIDPYMNKFTRWLLQYLNSYEDEELGMHADKRRRTDSWDMP

Target ORF information:

RefSeq Version	NM_001031897.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster meiotic 218 (mei-218), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001031897.3

ORF Insert Sequence:

ATGTCATCTA CGAATAAACT AGAGAAAAAG GACCCTCGTA GGCACATTCC CCTCCAGCTT 
AATGTTTTCT TGCGCAACGA TGACGATGGT GAAGTTCGTG AAGTTACTCA AAATGCCAAC 
ACAAGCTCCA TAGGCACCAT ACCATCGAAT GGCCTGGTCG AAAGCGATGT GACGCCTATT 
ATGACTCCCA CACCGAAGGC CGGTGTCTAC AAACCAAATG TTAACGTCTG GCGCTTATCT 
AAAGAAGTCA CTAAGCCTGA TCGTAGCTTT GTTGTGAGTG CCGAAGAGTA TCCTGATTTG 
GGCCAGCGCC CGAAGAGATT CAAACAACGA ACGCCGCGGG TGCGTAATCA AGATGACGGC 
GGCACTGTTT CGTGCCTTGC CAGTCGTTTT AGTCGCGTAA AAGATCCGCC TGTGCTGGGT 
AACTTTTTGC CGGATTTCAT GCTTCCGAAA TCGAGCAACA GCAATCATCA ATCGGTGACC 
ACTTGGCTGT CGTCAGTACG TCCTAGCGAC AGCCAAAAGT CTTTAAGCGA CCAGGCACGT 
TGCTCTTCTT CGACCAATAT AACACTGCTG AGTAAAGATT CAGTACAGCC GCTGACGCCA 
AAAAGTTCTG CCCAGTCCTC TCCGGGTGAG AAGAATATCC AACAGGAGCA GAATACTCCA 
GCTAAATCCC AACAGGAGGA GACAAGTCCG GCTAATTCTG TCGAGCATCA GCAGAAAACA 
CCTAACTCTC CAAAGGAGCA GCAGAAGAGT TCTGTCGGTT TTACTAACAT GCAGCAGATG 
GCTTCCGCCA ATTCTTTACA GCAGCAAAAC AGTTCTGCCA GTTCCTTACA GCAGCAGAAT 
AGTTCGGTCC ATTCCTTACA GAAGCGGAAG AGTTGGGTCA ATTCTTCACA GCAGCAGAGG 
AGTTCGGTCA ATTTCATACA GAAGCAAAGG AGTTGGGTCA ATTCCTCGCA TCATCAGAAG 
AGTTCTGCCA GTTCTTTACA GCAGAAGAGT TCTGAAAATT CTCCCAGCAA ATTCCAACAG 
CAGCAACAGA AGAGTCCTGC CAATAATGAA AGGAGCCAAA ACAATCAGAA GTCTCCTGGC 
ATTCAGCACT CTTGGAAACA ACAACAGCAG CAGAAGCCAA AGATTCTTCA GAATTGTCCG 
AAAACTTCTG TTCAGCAGCA GATGAACCCT GTTAATCATA CTCAGTCGCA ATCAGAGAGA 
CCTACCGAGC TCATTTACTT GCACTCAAGG AGCCCTGCGA AGAGAGCTTC AGTTACAAAC 
AATTCTTCGG CACCAATCCG CATTGCGCCC AGACAGCAGA CCACTCAATC TTCGGGTCCC 
AGTGTTGTCC ACAATGAGGA CGAGGATGTT ATGCCGCCGA CGCCTAATCA AAGTGAGAGA 
AGGCAGAGGA GATTCCCACG TCGCAATCAC AACCCCCGGA TGGTGCGCAG TCTGCAGGCG 
GTAAGTCGAG AGTTTACAGA GTCCTTGCTT GAGGGATCTT CAGGGAAAAC GCCGGAAAAA 
GAGGTGGTGC CGTACCAGCA ACAGGAGAAA CCGCGCCAGG TGAAAAGCTC ACCAGAAATC 
CATGCGAGCA GCCACATGCG AAAATTGGCT ATGGGCAGCG TTTTGAACTC AAATGCTAAG 
GAGTTCCACC CTACCGGAGA ATCCCATCTA ACCCACAAAG TGTCTAGATT GAGCCAAAGA 
CGCTGTCGAC AGCGTTTTAC GCGCTCTTTG CACGCGAAAA AAAATAACCC ACGCGAGCAA 
CTACCAAATC CTGACAGCGC CCCAGAACCA ATACTCGAAT ACACCTCTGA GATAGATGAT 
CAGCCATGTC CACCACGACA GACAGTCGAC ACACCGATCG AAGCCATTGT ACCACCGGCA 
ATGATCGAGG TGAGTGCCAC CGGCAATCTG CCGCCCGTGT TCTATTTTGA TCAGCAGTCG 
GGAATTATAT GGAGCAACTC GTCACCACAA ATTCCGGTTG AAATCCAAAG CAACAGTGTC 
AGCCAGTCGG TTTTTCGGCT AGCCAGTCTC CCGTGTACAG GGGTTGGCAC CACCAATATG 
GATATATTTA CCAGTTCGCC ACCCCCCATC CAGCATATGA GCTCCAGCCG CCTCCCATCC 
AGCATTTCCG GGCCCACCCA GTTATCGCAT ACTCTCAGTT CGCCGCCGGT GGAGAACCAG 
CACCAGCAAC ACTTGTGGGA ATCATGCATC CACCAGGACG AGGACCTAAT GTACAGCAGG 
CCTCTGTTGG ATATGGATCA GGAGGAAACC CAGCCGGAGC AACAGGACCA GGAATGGCAG 
TGGGAGTCGG AGATAGAGCC CCCGAGTTTC CAGTCGGAGA ACCAGGTCGT CGCCGACGAA 
CACGGCGAGG AAAACGCGTA CCTCGCTAAG GACGCAGGAC CACCACTCGA GCGTCATATG 
ATATTTCGAG CCCCATCACA GCACGGCCAA GAATATCCCA AGCCGGATCT AAACTGCGTG 
CCGTCCAGCA TCAAGAAACT ACATCACTTG ATCTCATCGC AGTATTCTGA CTACTCCTTT 
GTCTACGCCC TAAGTGCTCA GATCAGTCAG GACTGTGTGC CTATGGATTG CTTTGTGTAC 
CTGAAGATGA TACTGCTGGC CAGTATCGTT TCAATCGAAT CTGATGAGGT GCGGGCTCCA 
ATTTCGCTAT GCATAATCGC CACCGACAGC CTGATGGCCA ACCGTCTACT TAACAAAGTG 
GGCCAACTGG CACCACGTTT CCTGGGACCC CACGAATACG GACTGCAGCC CACTTTCAAT 
GCCCTGCCCA CGCGCTTTAA TTGGATAGTG GCCAGTCCTT TACTATTGGC ACAACAGGGA 
GTTTACTACG CCGGCGATTG GAATCGTCTG TCCAAGGATC AGGGATGCCA GCTGGAAAAG 
TGCATCGAAA ATGGAGCGGT CCCAGTTCCG CAGTTGCACA TCGATCAGCC CTTAAAGGCA 
GCCGTTTGGA CTTACTGGCA GCCGAACAAC TCCACCAATC AAACACTGGC CCTCGCCAAA 
CTGTGCCCAA TCTTCGGGCT GCCAATTTAT ATGGGTGCTC AGGCCAGCAA TAGTCTCTGG 
AACTCTATAA TTCAGAAGCA CAACGCTGAG GGTCAAATTG TAGTCAACGA TGGCTTGAGC 
ATTCCCGAGG ACGATATGCG CATGCTTATA CATCTGTTGC ATCAGCGCAA GACCACCTTA 
ACCGATGGCG CTCAGCATAT GCTGCAAAAG TATTATGTGA TCTCCAGGAA AGAGCGACCG 
AATGTCTTCT CTAGCAAGAC CTATATAGTG CTGAAGCAAT TAGCTGAGTG CTTTGCCAAG 
CTGGCGCTGC GGCTGGAGGT CCTTGAATCG GACGTTTGTG TGGCCATCTT TCACTGCGAG 
CATTTCGTGC AGGGCATTTT CGGGGCCAAA GAAAACCAGG CACCGCCGGC GGTCTTCAAT 
TTCAATGTGA TCTCCTGCAT CGATCCGTAC ATGAACAAGT TTACACGCTG GTTACTTCAG 
TATCTGAATA GCTACGAGGA CGAGGAGCTG GGGATGCACG CGGATAAGAG GCGGCGCACA 
GATAGCTGGG ACATGCCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mei-218 ( NM_001144743.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001138215.1 Drosophila melanogaster meiotic 218 (mei-218), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa02609
Clone ID Related Accession (Same CDS sequence)	NM_001031897.3 , NM_001144743.2 , NM_167557.3 , NM_001201728.1
Accession Version	NM_001144743.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	meiotic 218, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Oct 19, 2015 this sequence version replaced NM_001144743.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCATCTA CGAATAAACT AGAGAAAAAG GACCCTCGTA GGCACATTCC CCTCCAGCTT 
AATGTTTTCT TGCGCAACGA TGACGATGGT GAAGTTCGTG AAGTTACTCA AAATGCCAAC 
ACAAGCTCCA TAGGCACCAT ACCATCGAAT GGCCTGGTCG AAAGCGATGT GACGCCTATT 
ATGACTCCCA CACCGAAGGC CGGTGTCTAC AAACCAAATG TTAACGTCTG GCGCTTATCT 
AAAGAAGTCA CTAAGCCTGA TCGTAGCTTT GTTGTGAGTG CCGAAGAGTA TCCTGATTTG 
GGCCAGCGCC CGAAGAGATT CAAACAACGA ACGCCGCGGG TGCGTAATCA AGATGACGGC 
GGCACTGTTT CGTGCCTTGC CAGTCGTTTT AGTCGCGTAA AAGATCCGCC TGTGCTGGGT 
AACTTTTTGC CGGATTTCAT GCTTCCGAAA TCGAGCAACA GCAATCATCA ATCGGTGACC 
ACTTGGCTGT CGTCAGTACG TCCTAGCGAC AGCCAAAAGT CTTTAAGCGA CCAGGCACGT 
TGCTCTTCTT CGACCAATAT AACACTGCTG AGTAAAGATT CAGTACAGCC GCTGACGCCA 
AAAAGTTCTG CCCAGTCCTC TCCGGGTGAG AAGAATATCC AACAGGAGCA GAATACTCCA 
GCTAAATCCC AACAGGAGGA GACAAGTCCG GCTAATTCTG TCGAGCATCA GCAGAAAACA 
CCTAACTCTC CAAAGGAGCA GCAGAAGAGT TCTGTCGGTT TTACTAACAT GCAGCAGATG 
GCTTCCGCCA ATTCTTTACA GCAGCAAAAC AGTTCTGCCA GTTCCTTACA GCAGCAGAAT 
AGTTCGGTCC ATTCCTTACA GAAGCGGAAG AGTTGGGTCA ATTCTTCACA GCAGCAGAGG 
AGTTCGGTCA ATTTCATACA GAAGCAAAGG AGTTGGGTCA ATTCCTCGCA TCATCAGAAG 
AGTTCTGCCA GTTCTTTACA GCAGAAGAGT TCTGAAAATT CTCCCAGCAA ATTCCAACAG 
CAGCAACAGA AGAGTCCTGC CAATAATGAA AGGAGCCAAA ACAATCAGAA GTCTCCTGGC 
ATTCAGCACT CTTGGAAACA ACAACAGCAG CAGAAGCCAA AGATTCTTCA GAATTGTCCG 
AAAACTTCTG TTCAGCAGCA GATGAACCCT GTTAATCATA CTCAGTCGCA ATCAGAGAGA 
CCTACCGAGC TCATTTACTT GCACTCAAGG AGCCCTGCGA AGAGAGCTTC AGTTACAAAC 
AATTCTTCGG CACCAATCCG CATTGCGCCC AGACAGCAGA CCACTCAATC TTCGGGTCCC 
AGTGTTGTCC ACAATGAGGA CGAGGATGTT ATGCCGCCGA CGCCTAATCA AAGTGAGAGA 
AGGCAGAGGA GATTCCCACG TCGCAATCAC AACCCCCGGA TGGTGCGCAG TCTGCAGGCG 
GTAAGTCGAG AGTTTACAGA GTCCTTGCTT GAGGGATCTT CAGGGAAAAC GCCGGAAAAA 
GAGGTGGTGC CGTACCAGCA ACAGGAGAAA CCGCGCCAGG TGAAAAGCTC ACCAGAAATC 
CATGCGAGCA GCCACATGCG AAAATTGGCT ATGGGCAGCG TTTTGAACTC AAATGCTAAG 
GAGTTCCACC CTACCGGAGA ATCCCATCTA ACCCACAAAG TGTCTAGATT GAGCCAAAGA 
CGCTGTCGAC AGCGTTTTAC GCGCTCTTTG CACGCGAAAA AAAATAACCC ACGCGAGCAA 
CTACCAAATC CTGACAGCGC CCCAGAACCA ATACTCGAAT ACACCTCTGA GATAGATGAT 
CAGCCATGTC CACCACGACA GACAGTCGAC ACACCGATCG AAGCCATTGT ACCACCGGCA 
ATGATCGAGG TGAGTGCCAC CGGCAATCTG CCGCCCGTGT TCTATTTTGA TCAGCAGTCG 
GGAATTATAT GGAGCAACTC GTCACCACAA ATTCCGGTTG AAATCCAAAG CAACAGTGTC 
AGCCAGTCGG TTTTTCGGCT AGCCAGTCTC CCGTGTACAG GGGTTGGCAC CACCAATATG 
GATATATTTA CCAGTTCGCC ACCCCCCATC CAGCATATGA GCTCCAGCCG CCTCCCATCC 
AGCATTTCCG GGCCCACCCA GTTATCGCAT ACTCTCAGTT CGCCGCCGGT GGAGAACCAG 
CACCAGCAAC ACTTGTGGGA ATCATGCATC CACCAGGACG AGGACCTAAT GTACAGCAGG 
CCTCTGTTGG ATATGGATCA GGAGGAAACC CAGCCGGAGC AACAGGACCA GGAATGGCAG 
TGGGAGTCGG AGATAGAGCC CCCGAGTTTC CAGTCGGAGA ACCAGGTCGT CGCCGACGAA 
CACGGCGAGG AAAACGCGTA CCTCGCTAAG GACGCAGGAC CACCACTCGA GCGTCATATG 
ATATTTCGAG CCCCATCACA GCACGGCCAA GAATATCCCA AGCCGGATCT AAACTGCGTG 
CCGTCCAGCA TCAAGAAACT ACATCACTTG ATCTCATCGC AGTATTCTGA CTACTCCTTT 
GTCTACGCCC TAAGTGCTCA GATCAGTCAG GACTGTGTGC CTATGGATTG CTTTGTGTAC 
CTGAAGATGA TACTGCTGGC CAGTATCGTT TCAATCGAAT CTGATGAGGT GCGGGCTCCA 
ATTTCGCTAT GCATAATCGC CACCGACAGC CTGATGGCCA ACCGTCTACT TAACAAAGTG 
GGCCAACTGG CACCACGTTT CCTGGGACCC CACGAATACG GACTGCAGCC CACTTTCAAT 
GCCCTGCCCA CGCGCTTTAA TTGGATAGTG GCCAGTCCTT TACTATTGGC ACAACAGGGA 
GTTTACTACG CCGGCGATTG GAATCGTCTG TCCAAGGATC AGGGATGCCA GCTGGAAAAG 
TGCATCGAAA ATGGAGCGGT CCCAGTTCCG CAGTTGCACA TCGATCAGCC CTTAAAGGCA 
GCCGTTTGGA CTTACTGGCA GCCGAACAAC TCCACCAATC AAACACTGGC CCTCGCCAAA 
CTGTGCCCAA TCTTCGGGCT GCCAATTTAT ATGGGTGCTC AGGCCAGCAA TAGTCTCTGG 
AACTCTATAA TTCAGAAGCA CAACGCTGAG GGTCAAATTG TAGTCAACGA TGGCTTGAGC 
ATTCCCGAGG ACGATATGCG CATGCTTATA CATCTGTTGC ATCAGCGCAA GACCACCTTA 
ACCGATGGCG CTCAGCATAT GCTGCAAAAG TATTATGTGA TCTCCAGGAA AGAGCGACCG 
AATGTCTTCT CTAGCAAGAC CTATATAGTG CTGAAGCAAT TAGCTGAGTG CTTTGCCAAG 
CTGGCGCTGC GGCTGGAGGT CCTTGAATCG GACGTTTGTG TGGCCATCTT TCACTGCGAG 
CATTTCGTGC AGGGCATTTT CGGGGCCAAA GAAAACCAGG CACCGCCGGC GGTCTTCAAT 
TTCAATGTGA TCTCCTGCAT CGATCCGTAC ATGAACAAGT TTACACGCTG GTTACTTCAG 
TATCTGAATA GCTACGAGGA CGAGGAGCTG GGGATGCACG CGGATAAGAG GCGGCGCACA 
GATAGCTGGG ACATGCCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138215.1
CDS	1545..5105
Translation	MSSTNKLEKKDPRRHIPLQLNVFLRNDDDGEVREVTQNANTSSIGTIPSNGLVESDVTPIMTPTPKAGVYKPNVNVWRLSKEVTKPDRSFVVSAEEYPDLGQRPKRFKQRTPRVRNQDDGGTVSCLASRFSRVKDPPVLGNFLPDFMLPKSSNSNHQSVTTWLSSVRPSDSQKSLSDQARCSSSTNITLLSKDSVQPLTPKSSAQSSPGEKNIQQEQNTPAKSQQEETSPANSVEHQQKTPNSPKEQQKSSVGFTNMQQMASANSLQQQNSSASSLQQQNSSVHSLQKRKSWVNSSQQQRSSVNFIQKQRSWVNSSHHQKSSASSLQQKSSENSPSKFQQQQQKSPANNERSQNNQKSPGIQHSWKQQQQQKPKILQNCPKTSVQQQMNPVNHTQSQSERPTELIYLHSRSPAKRASVTNNSSAPIRIAPRQQTTQSSGPSVVHNEDEDVMPPTPNQSERRQRRFPRRNHNPRMVRSLQAVSREFTESLLEGSSGKTPEKEVVPYQQQEKPRQVKSSPEIHASSHMRKLAMGSVLNSNAKEFHPTGESHLTHKVSRLSQRRCRQRFTRSLHAKKNNPREQLPNPDSAPEPILEYTSEIDDQPCPPRQTVDTPIEAIVPPAMIEVSATGNLPPVFYFDQQSGIIWSNSSPQIPVEIQSNSVSQSVFRLASLPCTGVGTTNMDIFTSSPPPIQHMSSSRLPSSISGPTQLSHTLSSPPVENQHQQHLWESCIHQDEDLMYSRPLLDMDQEETQPEQQDQEWQWESEIEPPSFQSENQVVADEHGEENAYLAKDAGPPLERHMIFRAPSQHGQEYPKPDLNCVPSSIKKLHHLISSQYSDYSFVYALSAQISQDCVPMDCFVYLKMILLASIVSIESDEVRAPISLCIIATDSLMANRLLNKVGQLAPRFLGPHEYGLQPTFNALPTRFNWIVASPLLLAQQGVYYAGDWNRLSKDQGCQLEKCIENGAVPVPQLHIDQPLKAAVWTYWQPNNSTNQTLALAKLCPIFGLPIYMGAQASNSLWNSIIQKHNAEGQIVVNDGLSIPEDDMRMLIHLLHQRKTTLTDGAQHMLQKYYVISRKERPNVFSSKTYIVLKQLAECFAKLALRLEVLESDVCVAIFHCEHFVQGIFGAKENQAPPAVFNFNVISCIDPYMNKFTRWLLQYLNSYEDEELGMHADKRRRTDSWDMP

Target ORF information:

RefSeq Version	NM_001144743.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster meiotic 218 (mei-218), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144743.2

ORF Insert Sequence:

ATGTCATCTA CGAATAAACT AGAGAAAAAG GACCCTCGTA GGCACATTCC CCTCCAGCTT 
AATGTTTTCT TGCGCAACGA TGACGATGGT GAAGTTCGTG AAGTTACTCA AAATGCCAAC 
ACAAGCTCCA TAGGCACCAT ACCATCGAAT GGCCTGGTCG AAAGCGATGT GACGCCTATT 
ATGACTCCCA CACCGAAGGC CGGTGTCTAC AAACCAAATG TTAACGTCTG GCGCTTATCT 
AAAGAAGTCA CTAAGCCTGA TCGTAGCTTT GTTGTGAGTG CCGAAGAGTA TCCTGATTTG 
GGCCAGCGCC CGAAGAGATT CAAACAACGA ACGCCGCGGG TGCGTAATCA AGATGACGGC 
GGCACTGTTT CGTGCCTTGC CAGTCGTTTT AGTCGCGTAA AAGATCCGCC TGTGCTGGGT 
AACTTTTTGC CGGATTTCAT GCTTCCGAAA TCGAGCAACA GCAATCATCA ATCGGTGACC 
ACTTGGCTGT CGTCAGTACG TCCTAGCGAC AGCCAAAAGT CTTTAAGCGA CCAGGCACGT 
TGCTCTTCTT CGACCAATAT AACACTGCTG AGTAAAGATT CAGTACAGCC GCTGACGCCA 
AAAAGTTCTG CCCAGTCCTC TCCGGGTGAG AAGAATATCC AACAGGAGCA GAATACTCCA 
GCTAAATCCC AACAGGAGGA GACAAGTCCG GCTAATTCTG TCGAGCATCA GCAGAAAACA 
CCTAACTCTC CAAAGGAGCA GCAGAAGAGT TCTGTCGGTT TTACTAACAT GCAGCAGATG 
GCTTCCGCCA ATTCTTTACA GCAGCAAAAC AGTTCTGCCA GTTCCTTACA GCAGCAGAAT 
AGTTCGGTCC ATTCCTTACA GAAGCGGAAG AGTTGGGTCA ATTCTTCACA GCAGCAGAGG 
AGTTCGGTCA ATTTCATACA GAAGCAAAGG AGTTGGGTCA ATTCCTCGCA TCATCAGAAG 
AGTTCTGCCA GTTCTTTACA GCAGAAGAGT TCTGAAAATT CTCCCAGCAA ATTCCAACAG 
CAGCAACAGA AGAGTCCTGC CAATAATGAA AGGAGCCAAA ACAATCAGAA GTCTCCTGGC 
ATTCAGCACT CTTGGAAACA ACAACAGCAG CAGAAGCCAA AGATTCTTCA GAATTGTCCG 
AAAACTTCTG TTCAGCAGCA GATGAACCCT GTTAATCATA CTCAGTCGCA ATCAGAGAGA 
CCTACCGAGC TCATTTACTT GCACTCAAGG AGCCCTGCGA AGAGAGCTTC AGTTACAAAC 
AATTCTTCGG CACCAATCCG CATTGCGCCC AGACAGCAGA CCACTCAATC TTCGGGTCCC 
AGTGTTGTCC ACAATGAGGA CGAGGATGTT ATGCCGCCGA CGCCTAATCA AAGTGAGAGA 
AGGCAGAGGA GATTCCCACG TCGCAATCAC AACCCCCGGA TGGTGCGCAG TCTGCAGGCG 
GTAAGTCGAG AGTTTACAGA GTCCTTGCTT GAGGGATCTT CAGGGAAAAC GCCGGAAAAA 
GAGGTGGTGC CGTACCAGCA ACAGGAGAAA CCGCGCCAGG TGAAAAGCTC ACCAGAAATC 
CATGCGAGCA GCCACATGCG AAAATTGGCT ATGGGCAGCG TTTTGAACTC AAATGCTAAG 
GAGTTCCACC CTACCGGAGA ATCCCATCTA ACCCACAAAG TGTCTAGATT GAGCCAAAGA 
CGCTGTCGAC AGCGTTTTAC GCGCTCTTTG CACGCGAAAA AAAATAACCC ACGCGAGCAA 
CTACCAAATC CTGACAGCGC CCCAGAACCA ATACTCGAAT ACACCTCTGA GATAGATGAT 
CAGCCATGTC CACCACGACA GACAGTCGAC ACACCGATCG AAGCCATTGT ACCACCGGCA 
ATGATCGAGG TGAGTGCCAC CGGCAATCTG CCGCCCGTGT TCTATTTTGA TCAGCAGTCG 
GGAATTATAT GGAGCAACTC GTCACCACAA ATTCCGGTTG AAATCCAAAG CAACAGTGTC 
AGCCAGTCGG TTTTTCGGCT AGCCAGTCTC CCGTGTACAG GGGTTGGCAC CACCAATATG 
GATATATTTA CCAGTTCGCC ACCCCCCATC CAGCATATGA GCTCCAGCCG CCTCCCATCC 
AGCATTTCCG GGCCCACCCA GTTATCGCAT ACTCTCAGTT CGCCGCCGGT GGAGAACCAG 
CACCAGCAAC ACTTGTGGGA ATCATGCATC CACCAGGACG AGGACCTAAT GTACAGCAGG 
CCTCTGTTGG ATATGGATCA GGAGGAAACC CAGCCGGAGC AACAGGACCA GGAATGGCAG 
TGGGAGTCGG AGATAGAGCC CCCGAGTTTC CAGTCGGAGA ACCAGGTCGT CGCCGACGAA 
CACGGCGAGG AAAACGCGTA CCTCGCTAAG GACGCAGGAC CACCACTCGA GCGTCATATG 
ATATTTCGAG CCCCATCACA GCACGGCCAA GAATATCCCA AGCCGGATCT AAACTGCGTG 
CCGTCCAGCA TCAAGAAACT ACATCACTTG ATCTCATCGC AGTATTCTGA CTACTCCTTT 
GTCTACGCCC TAAGTGCTCA GATCAGTCAG GACTGTGTGC CTATGGATTG CTTTGTGTAC 
CTGAAGATGA TACTGCTGGC CAGTATCGTT TCAATCGAAT CTGATGAGGT GCGGGCTCCA 
ATTTCGCTAT GCATAATCGC CACCGACAGC CTGATGGCCA ACCGTCTACT TAACAAAGTG 
GGCCAACTGG CACCACGTTT CCTGGGACCC CACGAATACG GACTGCAGCC CACTTTCAAT 
GCCCTGCCCA CGCGCTTTAA TTGGATAGTG GCCAGTCCTT TACTATTGGC ACAACAGGGA 
GTTTACTACG CCGGCGATTG GAATCGTCTG TCCAAGGATC AGGGATGCCA GCTGGAAAAG 
TGCATCGAAA ATGGAGCGGT CCCAGTTCCG CAGTTGCACA TCGATCAGCC CTTAAAGGCA 
GCCGTTTGGA CTTACTGGCA GCCGAACAAC TCCACCAATC AAACACTGGC CCTCGCCAAA 
CTGTGCCCAA TCTTCGGGCT GCCAATTTAT ATGGGTGCTC AGGCCAGCAA TAGTCTCTGG 
AACTCTATAA TTCAGAAGCA CAACGCTGAG GGTCAAATTG TAGTCAACGA TGGCTTGAGC 
ATTCCCGAGG ACGATATGCG CATGCTTATA CATCTGTTGC ATCAGCGCAA GACCACCTTA 
ACCGATGGCG CTCAGCATAT GCTGCAAAAG TATTATGTGA TCTCCAGGAA AGAGCGACCG 
AATGTCTTCT CTAGCAAGAC CTATATAGTG CTGAAGCAAT TAGCTGAGTG CTTTGCCAAG 
CTGGCGCTGC GGCTGGAGGT CCTTGAATCG GACGTTTGTG TGGCCATCTT TCACTGCGAG 
CATTTCGTGC AGGGCATTTT CGGGGCCAAA GAAAACCAGG CACCGCCGGC GGTCTTCAAT 
TTCAATGTGA TCTCCTGCAT CGATCCGTAC ATGAACAAGT TTACACGCTG GTTACTTCAG 
TATCTGAATA GCTACGAGGA CGAGGAGCTG GGGATGCACG CGGATAAGAG GCGGCGCACA 
GATAGCTGGG ACATGCCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mei-218 ( NM_167557.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728052.2 Drosophila melanogaster meiotic 218 (mei-218), transcript variant B, mRNA.

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CloneID	OFa02609
Clone ID Related Accession (Same CDS sequence)	NM_001031897.3 , NM_001144743.2 , NM_167557.3 , NM_001201728.1
Accession Version	NM_167557.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	meiotic 218, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Oct 19, 2015 this sequence version replaced NM_167557.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCATCTA CGAATAAACT AGAGAAAAAG GACCCTCGTA GGCACATTCC CCTCCAGCTT 
AATGTTTTCT TGCGCAACGA TGACGATGGT GAAGTTCGTG AAGTTACTCA AAATGCCAAC 
ACAAGCTCCA TAGGCACCAT ACCATCGAAT GGCCTGGTCG AAAGCGATGT GACGCCTATT 
ATGACTCCCA CACCGAAGGC CGGTGTCTAC AAACCAAATG TTAACGTCTG GCGCTTATCT 
AAAGAAGTCA CTAAGCCTGA TCGTAGCTTT GTTGTGAGTG CCGAAGAGTA TCCTGATTTG 
GGCCAGCGCC CGAAGAGATT CAAACAACGA ACGCCGCGGG TGCGTAATCA AGATGACGGC 
GGCACTGTTT CGTGCCTTGC CAGTCGTTTT AGTCGCGTAA AAGATCCGCC TGTGCTGGGT 
AACTTTTTGC CGGATTTCAT GCTTCCGAAA TCGAGCAACA GCAATCATCA ATCGGTGACC 
ACTTGGCTGT CGTCAGTACG TCCTAGCGAC AGCCAAAAGT CTTTAAGCGA CCAGGCACGT 
TGCTCTTCTT CGACCAATAT AACACTGCTG AGTAAAGATT CAGTACAGCC GCTGACGCCA 
AAAAGTTCTG CCCAGTCCTC TCCGGGTGAG AAGAATATCC AACAGGAGCA GAATACTCCA 
GCTAAATCCC AACAGGAGGA GACAAGTCCG GCTAATTCTG TCGAGCATCA GCAGAAAACA 
CCTAACTCTC CAAAGGAGCA GCAGAAGAGT TCTGTCGGTT TTACTAACAT GCAGCAGATG 
GCTTCCGCCA ATTCTTTACA GCAGCAAAAC AGTTCTGCCA GTTCCTTACA GCAGCAGAAT 
AGTTCGGTCC ATTCCTTACA GAAGCGGAAG AGTTGGGTCA ATTCTTCACA GCAGCAGAGG 
AGTTCGGTCA ATTTCATACA GAAGCAAAGG AGTTGGGTCA ATTCCTCGCA TCATCAGAAG 
AGTTCTGCCA GTTCTTTACA GCAGAAGAGT TCTGAAAATT CTCCCAGCAA ATTCCAACAG 
CAGCAACAGA AGAGTCCTGC CAATAATGAA AGGAGCCAAA ACAATCAGAA GTCTCCTGGC 
ATTCAGCACT CTTGGAAACA ACAACAGCAG CAGAAGCCAA AGATTCTTCA GAATTGTCCG 
AAAACTTCTG TTCAGCAGCA GATGAACCCT GTTAATCATA CTCAGTCGCA ATCAGAGAGA 
CCTACCGAGC TCATTTACTT GCACTCAAGG AGCCCTGCGA AGAGAGCTTC AGTTACAAAC 
AATTCTTCGG CACCAATCCG CATTGCGCCC AGACAGCAGA CCACTCAATC TTCGGGTCCC 
AGTGTTGTCC ACAATGAGGA CGAGGATGTT ATGCCGCCGA CGCCTAATCA AAGTGAGAGA 
AGGCAGAGGA GATTCCCACG TCGCAATCAC AACCCCCGGA TGGTGCGCAG TCTGCAGGCG 
GTAAGTCGAG AGTTTACAGA GTCCTTGCTT GAGGGATCTT CAGGGAAAAC GCCGGAAAAA 
GAGGTGGTGC CGTACCAGCA ACAGGAGAAA CCGCGCCAGG TGAAAAGCTC ACCAGAAATC 
CATGCGAGCA GCCACATGCG AAAATTGGCT ATGGGCAGCG TTTTGAACTC AAATGCTAAG 
GAGTTCCACC CTACCGGAGA ATCCCATCTA ACCCACAAAG TGTCTAGATT GAGCCAAAGA 
CGCTGTCGAC AGCGTTTTAC GCGCTCTTTG CACGCGAAAA AAAATAACCC ACGCGAGCAA 
CTACCAAATC CTGACAGCGC CCCAGAACCA ATACTCGAAT ACACCTCTGA GATAGATGAT 
CAGCCATGTC CACCACGACA GACAGTCGAC ACACCGATCG AAGCCATTGT ACCACCGGCA 
ATGATCGAGG TGAGTGCCAC CGGCAATCTG CCGCCCGTGT TCTATTTTGA TCAGCAGTCG 
GGAATTATAT GGAGCAACTC GTCACCACAA ATTCCGGTTG AAATCCAAAG CAACAGTGTC 
AGCCAGTCGG TTTTTCGGCT AGCCAGTCTC CCGTGTACAG GGGTTGGCAC CACCAATATG 
GATATATTTA CCAGTTCGCC ACCCCCCATC CAGCATATGA GCTCCAGCCG CCTCCCATCC 
AGCATTTCCG GGCCCACCCA GTTATCGCAT ACTCTCAGTT CGCCGCCGGT GGAGAACCAG 
CACCAGCAAC ACTTGTGGGA ATCATGCATC CACCAGGACG AGGACCTAAT GTACAGCAGG 
CCTCTGTTGG ATATGGATCA GGAGGAAACC CAGCCGGAGC AACAGGACCA GGAATGGCAG 
TGGGAGTCGG AGATAGAGCC CCCGAGTTTC CAGTCGGAGA ACCAGGTCGT CGCCGACGAA 
CACGGCGAGG AAAACGCGTA CCTCGCTAAG GACGCAGGAC CACCACTCGA GCGTCATATG 
ATATTTCGAG CCCCATCACA GCACGGCCAA GAATATCCCA AGCCGGATCT AAACTGCGTG 
CCGTCCAGCA TCAAGAAACT ACATCACTTG ATCTCATCGC AGTATTCTGA CTACTCCTTT 
GTCTACGCCC TAAGTGCTCA GATCAGTCAG GACTGTGTGC CTATGGATTG CTTTGTGTAC 
CTGAAGATGA TACTGCTGGC CAGTATCGTT TCAATCGAAT CTGATGAGGT GCGGGCTCCA 
ATTTCGCTAT GCATAATCGC CACCGACAGC CTGATGGCCA ACCGTCTACT TAACAAAGTG 
GGCCAACTGG CACCACGTTT CCTGGGACCC CACGAATACG GACTGCAGCC CACTTTCAAT 
GCCCTGCCCA CGCGCTTTAA TTGGATAGTG GCCAGTCCTT TACTATTGGC ACAACAGGGA 
GTTTACTACG CCGGCGATTG GAATCGTCTG TCCAAGGATC AGGGATGCCA GCTGGAAAAG 
TGCATCGAAA ATGGAGCGGT CCCAGTTCCG CAGTTGCACA TCGATCAGCC CTTAAAGGCA 
GCCGTTTGGA CTTACTGGCA GCCGAACAAC TCCACCAATC AAACACTGGC CCTCGCCAAA 
CTGTGCCCAA TCTTCGGGCT GCCAATTTAT ATGGGTGCTC AGGCCAGCAA TAGTCTCTGG 
AACTCTATAA TTCAGAAGCA CAACGCTGAG GGTCAAATTG TAGTCAACGA TGGCTTGAGC 
ATTCCCGAGG ACGATATGCG CATGCTTATA CATCTGTTGC ATCAGCGCAA GACCACCTTA 
ACCGATGGCG CTCAGCATAT GCTGCAAAAG TATTATGTGA TCTCCAGGAA AGAGCGACCG 
AATGTCTTCT CTAGCAAGAC CTATATAGTG CTGAAGCAAT TAGCTGAGTG CTTTGCCAAG 
CTGGCGCTGC GGCTGGAGGT CCTTGAATCG GACGTTTGTG TGGCCATCTT TCACTGCGAG 
CATTTCGTGC AGGGCATTTT CGGGGCCAAA GAAAACCAGG CACCGCCGGC GGTCTTCAAT 
TTCAATGTGA TCTCCTGCAT CGATCCGTAC ATGAACAAGT TTACACGCTG GTTACTTCAG 
TATCTGAATA GCTACGAGGA CGAGGAGCTG GGGATGCACG CGGATAAGAG GCGGCGCACA 
GATAGCTGGG ACATGCCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728052.2
CDS	437..3997
Translation	MSSTNKLEKKDPRRHIPLQLNVFLRNDDDGEVREVTQNANTSSIGTIPSNGLVESDVTPIMTPTPKAGVYKPNVNVWRLSKEVTKPDRSFVVSAEEYPDLGQRPKRFKQRTPRVRNQDDGGTVSCLASRFSRVKDPPVLGNFLPDFMLPKSSNSNHQSVTTWLSSVRPSDSQKSLSDQARCSSSTNITLLSKDSVQPLTPKSSAQSSPGEKNIQQEQNTPAKSQQEETSPANSVEHQQKTPNSPKEQQKSSVGFTNMQQMASANSLQQQNSSASSLQQQNSSVHSLQKRKSWVNSSQQQRSSVNFIQKQRSWVNSSHHQKSSASSLQQKSSENSPSKFQQQQQKSPANNERSQNNQKSPGIQHSWKQQQQQKPKILQNCPKTSVQQQMNPVNHTQSQSERPTELIYLHSRSPAKRASVTNNSSAPIRIAPRQQTTQSSGPSVVHNEDEDVMPPTPNQSERRQRRFPRRNHNPRMVRSLQAVSREFTESLLEGSSGKTPEKEVVPYQQQEKPRQVKSSPEIHASSHMRKLAMGSVLNSNAKEFHPTGESHLTHKVSRLSQRRCRQRFTRSLHAKKNNPREQLPNPDSAPEPILEYTSEIDDQPCPPRQTVDTPIEAIVPPAMIEVSATGNLPPVFYFDQQSGIIWSNSSPQIPVEIQSNSVSQSVFRLASLPCTGVGTTNMDIFTSSPPPIQHMSSSRLPSSISGPTQLSHTLSSPPVENQHQQHLWESCIHQDEDLMYSRPLLDMDQEETQPEQQDQEWQWESEIEPPSFQSENQVVADEHGEENAYLAKDAGPPLERHMIFRAPSQHGQEYPKPDLNCVPSSIKKLHHLISSQYSDYSFVYALSAQISQDCVPMDCFVYLKMILLASIVSIESDEVRAPISLCIIATDSLMANRLLNKVGQLAPRFLGPHEYGLQPTFNALPTRFNWIVASPLLLAQQGVYYAGDWNRLSKDQGCQLEKCIENGAVPVPQLHIDQPLKAAVWTYWQPNNSTNQTLALAKLCPIFGLPIYMGAQASNSLWNSIIQKHNAEGQIVVNDGLSIPEDDMRMLIHLLHQRKTTLTDGAQHMLQKYYVISRKERPNVFSSKTYIVLKQLAECFAKLALRLEVLESDVCVAIFHCEHFVQGIFGAKENQAPPAVFNFNVISCIDPYMNKFTRWLLQYLNSYEDEELGMHADKRRRTDSWDMP

Target ORF information:

RefSeq Version	NM_167557.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster meiotic 218 (mei-218), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167557.3

ORF Insert Sequence:

ATGTCATCTA CGAATAAACT AGAGAAAAAG GACCCTCGTA GGCACATTCC CCTCCAGCTT 
AATGTTTTCT TGCGCAACGA TGACGATGGT GAAGTTCGTG AAGTTACTCA AAATGCCAAC 
ACAAGCTCCA TAGGCACCAT ACCATCGAAT GGCCTGGTCG AAAGCGATGT GACGCCTATT 
ATGACTCCCA CACCGAAGGC CGGTGTCTAC AAACCAAATG TTAACGTCTG GCGCTTATCT 
AAAGAAGTCA CTAAGCCTGA TCGTAGCTTT GTTGTGAGTG CCGAAGAGTA TCCTGATTTG 
GGCCAGCGCC CGAAGAGATT CAAACAACGA ACGCCGCGGG TGCGTAATCA AGATGACGGC 
GGCACTGTTT CGTGCCTTGC CAGTCGTTTT AGTCGCGTAA AAGATCCGCC TGTGCTGGGT 
AACTTTTTGC CGGATTTCAT GCTTCCGAAA TCGAGCAACA GCAATCATCA ATCGGTGACC 
ACTTGGCTGT CGTCAGTACG TCCTAGCGAC AGCCAAAAGT CTTTAAGCGA CCAGGCACGT 
TGCTCTTCTT CGACCAATAT AACACTGCTG AGTAAAGATT CAGTACAGCC GCTGACGCCA 
AAAAGTTCTG CCCAGTCCTC TCCGGGTGAG AAGAATATCC AACAGGAGCA GAATACTCCA 
GCTAAATCCC AACAGGAGGA GACAAGTCCG GCTAATTCTG TCGAGCATCA GCAGAAAACA 
CCTAACTCTC CAAAGGAGCA GCAGAAGAGT TCTGTCGGTT TTACTAACAT GCAGCAGATG 
GCTTCCGCCA ATTCTTTACA GCAGCAAAAC AGTTCTGCCA GTTCCTTACA GCAGCAGAAT 
AGTTCGGTCC ATTCCTTACA GAAGCGGAAG AGTTGGGTCA ATTCTTCACA GCAGCAGAGG 
AGTTCGGTCA ATTTCATACA GAAGCAAAGG AGTTGGGTCA ATTCCTCGCA TCATCAGAAG 
AGTTCTGCCA GTTCTTTACA GCAGAAGAGT TCTGAAAATT CTCCCAGCAA ATTCCAACAG 
CAGCAACAGA AGAGTCCTGC CAATAATGAA AGGAGCCAAA ACAATCAGAA GTCTCCTGGC 
ATTCAGCACT CTTGGAAACA ACAACAGCAG CAGAAGCCAA AGATTCTTCA GAATTGTCCG 
AAAACTTCTG TTCAGCAGCA GATGAACCCT GTTAATCATA CTCAGTCGCA ATCAGAGAGA 
CCTACCGAGC TCATTTACTT GCACTCAAGG AGCCCTGCGA AGAGAGCTTC AGTTACAAAC 
AATTCTTCGG CACCAATCCG CATTGCGCCC AGACAGCAGA CCACTCAATC TTCGGGTCCC 
AGTGTTGTCC ACAATGAGGA CGAGGATGTT ATGCCGCCGA CGCCTAATCA AAGTGAGAGA 
AGGCAGAGGA GATTCCCACG TCGCAATCAC AACCCCCGGA TGGTGCGCAG TCTGCAGGCG 
GTAAGTCGAG AGTTTACAGA GTCCTTGCTT GAGGGATCTT CAGGGAAAAC GCCGGAAAAA 
GAGGTGGTGC CGTACCAGCA ACAGGAGAAA CCGCGCCAGG TGAAAAGCTC ACCAGAAATC 
CATGCGAGCA GCCACATGCG AAAATTGGCT ATGGGCAGCG TTTTGAACTC AAATGCTAAG 
GAGTTCCACC CTACCGGAGA ATCCCATCTA ACCCACAAAG TGTCTAGATT GAGCCAAAGA 
CGCTGTCGAC AGCGTTTTAC GCGCTCTTTG CACGCGAAAA AAAATAACCC ACGCGAGCAA 
CTACCAAATC CTGACAGCGC CCCAGAACCA ATACTCGAAT ACACCTCTGA GATAGATGAT 
CAGCCATGTC CACCACGACA GACAGTCGAC ACACCGATCG AAGCCATTGT ACCACCGGCA 
ATGATCGAGG TGAGTGCCAC CGGCAATCTG CCGCCCGTGT TCTATTTTGA TCAGCAGTCG 
GGAATTATAT GGAGCAACTC GTCACCACAA ATTCCGGTTG AAATCCAAAG CAACAGTGTC 
AGCCAGTCGG TTTTTCGGCT AGCCAGTCTC CCGTGTACAG GGGTTGGCAC CACCAATATG 
GATATATTTA CCAGTTCGCC ACCCCCCATC CAGCATATGA GCTCCAGCCG CCTCCCATCC 
AGCATTTCCG GGCCCACCCA GTTATCGCAT ACTCTCAGTT CGCCGCCGGT GGAGAACCAG 
CACCAGCAAC ACTTGTGGGA ATCATGCATC CACCAGGACG AGGACCTAAT GTACAGCAGG 
CCTCTGTTGG ATATGGATCA GGAGGAAACC CAGCCGGAGC AACAGGACCA GGAATGGCAG 
TGGGAGTCGG AGATAGAGCC CCCGAGTTTC CAGTCGGAGA ACCAGGTCGT CGCCGACGAA 
CACGGCGAGG AAAACGCGTA CCTCGCTAAG GACGCAGGAC CACCACTCGA GCGTCATATG 
ATATTTCGAG CCCCATCACA GCACGGCCAA GAATATCCCA AGCCGGATCT AAACTGCGTG 
CCGTCCAGCA TCAAGAAACT ACATCACTTG ATCTCATCGC AGTATTCTGA CTACTCCTTT 
GTCTACGCCC TAAGTGCTCA GATCAGTCAG GACTGTGTGC CTATGGATTG CTTTGTGTAC 
CTGAAGATGA TACTGCTGGC CAGTATCGTT TCAATCGAAT CTGATGAGGT GCGGGCTCCA 
ATTTCGCTAT GCATAATCGC CACCGACAGC CTGATGGCCA ACCGTCTACT TAACAAAGTG 
GGCCAACTGG CACCACGTTT CCTGGGACCC CACGAATACG GACTGCAGCC CACTTTCAAT 
GCCCTGCCCA CGCGCTTTAA TTGGATAGTG GCCAGTCCTT TACTATTGGC ACAACAGGGA 
GTTTACTACG CCGGCGATTG GAATCGTCTG TCCAAGGATC AGGGATGCCA GCTGGAAAAG 
TGCATCGAAA ATGGAGCGGT CCCAGTTCCG CAGTTGCACA TCGATCAGCC CTTAAAGGCA 
GCCGTTTGGA CTTACTGGCA GCCGAACAAC TCCACCAATC AAACACTGGC CCTCGCCAAA 
CTGTGCCCAA TCTTCGGGCT GCCAATTTAT ATGGGTGCTC AGGCCAGCAA TAGTCTCTGG 
AACTCTATAA TTCAGAAGCA CAACGCTGAG GGTCAAATTG TAGTCAACGA TGGCTTGAGC 
ATTCCCGAGG ACGATATGCG CATGCTTATA CATCTGTTGC ATCAGCGCAA GACCACCTTA 
ACCGATGGCG CTCAGCATAT GCTGCAAAAG TATTATGTGA TCTCCAGGAA AGAGCGACCG 
AATGTCTTCT CTAGCAAGAC CTATATAGTG CTGAAGCAAT TAGCTGAGTG CTTTGCCAAG 
CTGGCGCTGC GGCTGGAGGT CCTTGAATCG GACGTTTGTG TGGCCATCTT TCACTGCGAG 
CATTTCGTGC AGGGCATTTT CGGGGCCAAA GAAAACCAGG CACCGCCGGC GGTCTTCAAT 
TTCAATGTGA TCTCCTGCAT CGATCCGTAC ATGAACAAGT TTACACGCTG GTTACTTCAG 
TATCTGAATA GCTACGAGGA CGAGGAGCTG GGGATGCACG CGGATAAGAG GCGGCGCACA 
GATAGCTGGG ACATGCCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mei-218 ( NM_001201728.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188657.1 Drosophila melanogaster meiotic 218 (mei-218), transcript variant E, mRNA.

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CloneID	OFa02609
Clone ID Related Accession (Same CDS sequence)	NM_001031897.3 , NM_001144743.2 , NM_167557.3 , NM_001201728.1
Accession Version	NM_001201728.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	meiotic 218, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCATCTA CGAATAAACT AGAGAAAAAG GACCCTCGTA GGCACATTCC CCTCCAGCTT 
AATGTTTTCT TGCGCAACGA TGACGATGGT GAAGTTCGTG AAGTTACTCA AAATGCCAAC 
ACAAGCTCCA TAGGCACCAT ACCATCGAAT GGCCTGGTCG AAAGCGATGT GACGCCTATT 
ATGACTCCCA CACCGAAGGC CGGTGTCTAC AAACCAAATG TTAACGTCTG GCGCTTATCT 
AAAGAAGTCA CTAAGCCTGA TCGTAGCTTT GTTGTGAGTG CCGAAGAGTA TCCTGATTTG 
GGCCAGCGCC CGAAGAGATT CAAACAACGA ACGCCGCGGG TGCGTAATCA AGATGACGGC 
GGCACTGTTT CGTGCCTTGC CAGTCGTTTT AGTCGCGTAA AAGATCCGCC TGTGCTGGGT 
AACTTTTTGC CGGATTTCAT GCTTCCGAAA TCGAGCAACA GCAATCATCA ATCGGTGACC 
ACTTGGCTGT CGTCAGTACG TCCTAGCGAC AGCCAAAAGT CTTTAAGCGA CCAGGCACGT 
TGCTCTTCTT CGACCAATAT AACACTGCTG AGTAAAGATT CAGTACAGCC GCTGACGCCA 
AAAAGTTCTG CCCAGTCCTC TCCGGGTGAG AAGAATATCC AACAGGAGCA GAATACTCCA 
GCTAAATCCC AACAGGAGGA GACAAGTCCG GCTAATTCTG TCGAGCATCA GCAGAAAACA 
CCTAACTCTC CAAAGGAGCA GCAGAAGAGT TCTGTCGGTT TTACTAACAT GCAGCAGATG 
GCTTCCGCCA ATTCTTTACA GCAGCAAAAC AGTTCTGCCA GTTCCTTACA GCAGCAGAAT 
AGTTCGGTCC ATTCCTTACA GAAGCGGAAG AGTTGGGTCA ATTCTTCACA GCAGCAGAGG 
AGTTCGGTCA ATTTCATACA GAAGCAAAGG AGTTGGGTCA ATTCCTCGCA TCATCAGAAG 
AGTTCTGCCA GTTCTTTACA GCAGAAGAGT TCTGAAAATT CTCCCAGCAA ATTCCAACAG 
CAGCAACAGA AGAGTCCTGC CAATAATGAA AGGAGCCAAA ACAATCAGAA GTCTCCTGGC 
ATTCAGCACT CTTGGAAACA ACAACAGCAG CAGAAGCCAA AGATTCTTCA GAATTGTCCG 
AAAACTTCTG TTCAGCAGCA GATGAACCCT GTTAATCATA CTCAGTCGCA ATCAGAGAGA 
CCTACCGAGC TCATTTACTT GCACTCAAGG AGCCCTGCGA AGAGAGCTTC AGTTACAAAC 
AATTCTTCGG CACCAATCCG CATTGCGCCC AGACAGCAGA CCACTCAATC TTCGGGTCCC 
AGTGTTGTCC ACAATGAGGA CGAGGATGTT ATGCCGCCGA CGCCTAATCA AAGTGAGAGA 
AGGCAGAGGA GATTCCCACG TCGCAATCAC AACCCCCGGA TGGTGCGCAG TCTGCAGGCG 
GTAAGTCGAG AGTTTACAGA GTCCTTGCTT GAGGGATCTT CAGGGAAAAC GCCGGAAAAA 
GAGGTGGTGC CGTACCAGCA ACAGGAGAAA CCGCGCCAGG TGAAAAGCTC ACCAGAAATC 
CATGCGAGCA GCCACATGCG AAAATTGGCT ATGGGCAGCG TTTTGAACTC AAATGCTAAG 
GAGTTCCACC CTACCGGAGA ATCCCATCTA ACCCACAAAG TGTCTAGATT GAGCCAAAGA 
CGCTGTCGAC AGCGTTTTAC GCGCTCTTTG CACGCGAAAA AAAATAACCC ACGCGAGCAA 
CTACCAAATC CTGACAGCGC CCCAGAACCA ATACTCGAAT ACACCTCTGA GATAGATGAT 
CAGCCATGTC CACCACGACA GACAGTCGAC ACACCGATCG AAGCCATTGT ACCACCGGCA 
ATGATCGAGG TGAGTGCCAC CGGCAATCTG CCGCCCGTGT TCTATTTTGA TCAGCAGTCG 
GGAATTATAT GGAGCAACTC GTCACCACAA ATTCCGGTTG AAATCCAAAG CAACAGTGTC 
AGCCAGTCGG TTTTTCGGCT AGCCAGTCTC CCGTGTACAG GGGTTGGCAC CACCAATATG 
GATATATTTA CCAGTTCGCC ACCCCCCATC CAGCATATGA GCTCCAGCCG CCTCCCATCC 
AGCATTTCCG GGCCCACCCA GTTATCGCAT ACTCTCAGTT CGCCGCCGGT GGAGAACCAG 
CACCAGCAAC ACTTGTGGGA ATCATGCATC CACCAGGACG AGGACCTAAT GTACAGCAGG 
CCTCTGTTGG ATATGGATCA GGAGGAAACC CAGCCGGAGC AACAGGACCA GGAATGGCAG 
TGGGAGTCGG AGATAGAGCC CCCGAGTTTC CAGTCGGAGA ACCAGGTCGT CGCCGACGAA 
CACGGCGAGG AAAACGCGTA CCTCGCTAAG GACGCAGGAC CACCACTCGA GCGTCATATG 
ATATTTCGAG CCCCATCACA GCACGGCCAA GAATATCCCA AGCCGGATCT AAACTGCGTG 
CCGTCCAGCA TCAAGAAACT ACATCACTTG ATCTCATCGC AGTATTCTGA CTACTCCTTT 
GTCTACGCCC TAAGTGCTCA GATCAGTCAG GACTGTGTGC CTATGGATTG CTTTGTGTAC 
CTGAAGATGA TACTGCTGGC CAGTATCGTT TCAATCGAAT CTGATGAGGT GCGGGCTCCA 
ATTTCGCTAT GCATAATCGC CACCGACAGC CTGATGGCCA ACCGTCTACT TAACAAAGTG 
GGCCAACTGG CACCACGTTT CCTGGGACCC CACGAATACG GACTGCAGCC CACTTTCAAT 
GCCCTGCCCA CGCGCTTTAA TTGGATAGTG GCCAGTCCTT TACTATTGGC ACAACAGGGA 
GTTTACTACG CCGGCGATTG GAATCGTCTG TCCAAGGATC AGGGATGCCA GCTGGAAAAG 
TGCATCGAAA ATGGAGCGGT CCCAGTTCCG CAGTTGCACA TCGATCAGCC CTTAAAGGCA 
GCCGTTTGGA CTTACTGGCA GCCGAACAAC TCCACCAATC AAACACTGGC CCTCGCCAAA 
CTGTGCCCAA TCTTCGGGCT GCCAATTTAT ATGGGTGCTC AGGCCAGCAA TAGTCTCTGG 
AACTCTATAA TTCAGAAGCA CAACGCTGAG GGTCAAATTG TAGTCAACGA TGGCTTGAGC 
ATTCCCGAGG ACGATATGCG CATGCTTATA CATCTGTTGC ATCAGCGCAA GACCACCTTA 
ACCGATGGCG CTCAGCATAT GCTGCAAAAG TATTATGTGA TCTCCAGGAA AGAGCGACCG 
AATGTCTTCT CTAGCAAGAC CTATATAGTG CTGAAGCAAT TAGCTGAGTG CTTTGCCAAG 
CTGGCGCTGC GGCTGGAGGT CCTTGAATCG GACGTTTGTG TGGCCATCTT TCACTGCGAG 
CATTTCGTGC AGGGCATTTT CGGGGCCAAA GAAAACCAGG CACCGCCGGC GGTCTTCAAT 
TTCAATGTGA TCTCCTGCAT CGATCCGTAC ATGAACAAGT TTACACGCTG GTTACTTCAG 
TATCTGAATA GCTACGAGGA CGAGGAGCTG GGGATGCACG CGGATAAGAG GCGGCGCACA 
GATAGCTGGG ACATGCCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188657.1
CDS	361..3921
Translation	MSSTNKLEKKDPRRHIPLQLNVFLRNDDDGEVREVTQNANTSSIGTIPSNGLVESDVTPIMTPTPKAGVYKPNVNVWRLSKEVTKPDRSFVVSAEEYPDLGQRPKRFKQRTPRVRNQDDGGTVSCLASRFSRVKDPPVLGNFLPDFMLPKSSNSNHQSVTTWLSSVRPSDSQKSLSDQARCSSSTNITLLSKDSVQPLTPKSSAQSSPGEKNIQQEQNTPAKSQQEETSPANSVEHQQKTPNSPKEQQKSSVGFTNMQQMASANSLQQQNSSASSLQQQNSSVHSLQKRKSWVNSSQQQRSSVNFIQKQRSWVNSSHHQKSSASSLQQKSSENSPSKFQQQQQKSPANNERSQNNQKSPGIQHSWKQQQQQKPKILQNCPKTSVQQQMNPVNHTQSQSERPTELIYLHSRSPAKRASVTNNSSAPIRIAPRQQTTQSSGPSVVHNEDEDVMPPTPNQSERRQRRFPRRNHNPRMVRSLQAVSREFTESLLEGSSGKTPEKEVVPYQQQEKPRQVKSSPEIHASSHMRKLAMGSVLNSNAKEFHPTGESHLTHKVSRLSQRRCRQRFTRSLHAKKNNPREQLPNPDSAPEPILEYTSEIDDQPCPPRQTVDTPIEAIVPPAMIEVSATGNLPPVFYFDQQSGIIWSNSSPQIPVEIQSNSVSQSVFRLASLPCTGVGTTNMDIFTSSPPPIQHMSSSRLPSSISGPTQLSHTLSSPPVENQHQQHLWESCIHQDEDLMYSRPLLDMDQEETQPEQQDQEWQWESEIEPPSFQSENQVVADEHGEENAYLAKDAGPPLERHMIFRAPSQHGQEYPKPDLNCVPSSIKKLHHLISSQYSDYSFVYALSAQISQDCVPMDCFVYLKMILLASIVSIESDEVRAPISLCIIATDSLMANRLLNKVGQLAPRFLGPHEYGLQPTFNALPTRFNWIVASPLLLAQQGVYYAGDWNRLSKDQGCQLEKCIENGAVPVPQLHIDQPLKAAVWTYWQPNNSTNQTLALAKLCPIFGLPIYMGAQASNSLWNSIIQKHNAEGQIVVNDGLSIPEDDMRMLIHLLHQRKTTLTDGAQHMLQKYYVISRKERPNVFSSKTYIVLKQLAECFAKLALRLEVLESDVCVAIFHCEHFVQGIFGAKENQAPPAVFNFNVISCIDPYMNKFTRWLLQYLNSYEDEELGMHADKRRRTDSWDMP

Target ORF information:

RefSeq Version	NM_001201728.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster meiotic 218 (mei-218), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201728.1

ORF Insert Sequence:

ATGTCATCTA CGAATAAACT AGAGAAAAAG GACCCTCGTA GGCACATTCC CCTCCAGCTT 
AATGTTTTCT TGCGCAACGA TGACGATGGT GAAGTTCGTG AAGTTACTCA AAATGCCAAC 
ACAAGCTCCA TAGGCACCAT ACCATCGAAT GGCCTGGTCG AAAGCGATGT GACGCCTATT 
ATGACTCCCA CACCGAAGGC CGGTGTCTAC AAACCAAATG TTAACGTCTG GCGCTTATCT 
AAAGAAGTCA CTAAGCCTGA TCGTAGCTTT GTTGTGAGTG CCGAAGAGTA TCCTGATTTG 
GGCCAGCGCC CGAAGAGATT CAAACAACGA ACGCCGCGGG TGCGTAATCA AGATGACGGC 
GGCACTGTTT CGTGCCTTGC CAGTCGTTTT AGTCGCGTAA AAGATCCGCC TGTGCTGGGT 
AACTTTTTGC CGGATTTCAT GCTTCCGAAA TCGAGCAACA GCAATCATCA ATCGGTGACC 
ACTTGGCTGT CGTCAGTACG TCCTAGCGAC AGCCAAAAGT CTTTAAGCGA CCAGGCACGT 
TGCTCTTCTT CGACCAATAT AACACTGCTG AGTAAAGATT CAGTACAGCC GCTGACGCCA 
AAAAGTTCTG CCCAGTCCTC TCCGGGTGAG AAGAATATCC AACAGGAGCA GAATACTCCA 
GCTAAATCCC AACAGGAGGA GACAAGTCCG GCTAATTCTG TCGAGCATCA GCAGAAAACA 
CCTAACTCTC CAAAGGAGCA GCAGAAGAGT TCTGTCGGTT TTACTAACAT GCAGCAGATG 
GCTTCCGCCA ATTCTTTACA GCAGCAAAAC AGTTCTGCCA GTTCCTTACA GCAGCAGAAT 
AGTTCGGTCC ATTCCTTACA GAAGCGGAAG AGTTGGGTCA ATTCTTCACA GCAGCAGAGG 
AGTTCGGTCA ATTTCATACA GAAGCAAAGG AGTTGGGTCA ATTCCTCGCA TCATCAGAAG 
AGTTCTGCCA GTTCTTTACA GCAGAAGAGT TCTGAAAATT CTCCCAGCAA ATTCCAACAG 
CAGCAACAGA AGAGTCCTGC CAATAATGAA AGGAGCCAAA ACAATCAGAA GTCTCCTGGC 
ATTCAGCACT CTTGGAAACA ACAACAGCAG CAGAAGCCAA AGATTCTTCA GAATTGTCCG 
AAAACTTCTG TTCAGCAGCA GATGAACCCT GTTAATCATA CTCAGTCGCA ATCAGAGAGA 
CCTACCGAGC TCATTTACTT GCACTCAAGG AGCCCTGCGA AGAGAGCTTC AGTTACAAAC 
AATTCTTCGG CACCAATCCG CATTGCGCCC AGACAGCAGA CCACTCAATC TTCGGGTCCC 
AGTGTTGTCC ACAATGAGGA CGAGGATGTT ATGCCGCCGA CGCCTAATCA AAGTGAGAGA 
AGGCAGAGGA GATTCCCACG TCGCAATCAC AACCCCCGGA TGGTGCGCAG TCTGCAGGCG 
GTAAGTCGAG AGTTTACAGA GTCCTTGCTT GAGGGATCTT CAGGGAAAAC GCCGGAAAAA 
GAGGTGGTGC CGTACCAGCA ACAGGAGAAA CCGCGCCAGG TGAAAAGCTC ACCAGAAATC 
CATGCGAGCA GCCACATGCG AAAATTGGCT ATGGGCAGCG TTTTGAACTC AAATGCTAAG 
GAGTTCCACC CTACCGGAGA ATCCCATCTA ACCCACAAAG TGTCTAGATT GAGCCAAAGA 
CGCTGTCGAC AGCGTTTTAC GCGCTCTTTG CACGCGAAAA AAAATAACCC ACGCGAGCAA 
CTACCAAATC CTGACAGCGC CCCAGAACCA ATACTCGAAT ACACCTCTGA GATAGATGAT 
CAGCCATGTC CACCACGACA GACAGTCGAC ACACCGATCG AAGCCATTGT ACCACCGGCA 
ATGATCGAGG TGAGTGCCAC CGGCAATCTG CCGCCCGTGT TCTATTTTGA TCAGCAGTCG 
GGAATTATAT GGAGCAACTC GTCACCACAA ATTCCGGTTG AAATCCAAAG CAACAGTGTC 
AGCCAGTCGG TTTTTCGGCT AGCCAGTCTC CCGTGTACAG GGGTTGGCAC CACCAATATG 
GATATATTTA CCAGTTCGCC ACCCCCCATC CAGCATATGA GCTCCAGCCG CCTCCCATCC 
AGCATTTCCG GGCCCACCCA GTTATCGCAT ACTCTCAGTT CGCCGCCGGT GGAGAACCAG 
CACCAGCAAC ACTTGTGGGA ATCATGCATC CACCAGGACG AGGACCTAAT GTACAGCAGG 
CCTCTGTTGG ATATGGATCA GGAGGAAACC CAGCCGGAGC AACAGGACCA GGAATGGCAG 
TGGGAGTCGG AGATAGAGCC CCCGAGTTTC CAGTCGGAGA ACCAGGTCGT CGCCGACGAA 
CACGGCGAGG AAAACGCGTA CCTCGCTAAG GACGCAGGAC CACCACTCGA GCGTCATATG 
ATATTTCGAG CCCCATCACA GCACGGCCAA GAATATCCCA AGCCGGATCT AAACTGCGTG 
CCGTCCAGCA TCAAGAAACT ACATCACTTG ATCTCATCGC AGTATTCTGA CTACTCCTTT 
GTCTACGCCC TAAGTGCTCA GATCAGTCAG GACTGTGTGC CTATGGATTG CTTTGTGTAC 
CTGAAGATGA TACTGCTGGC CAGTATCGTT TCAATCGAAT CTGATGAGGT GCGGGCTCCA 
ATTTCGCTAT GCATAATCGC CACCGACAGC CTGATGGCCA ACCGTCTACT TAACAAAGTG 
GGCCAACTGG CACCACGTTT CCTGGGACCC CACGAATACG GACTGCAGCC CACTTTCAAT 
GCCCTGCCCA CGCGCTTTAA TTGGATAGTG GCCAGTCCTT TACTATTGGC ACAACAGGGA 
GTTTACTACG CCGGCGATTG GAATCGTCTG TCCAAGGATC AGGGATGCCA GCTGGAAAAG 
TGCATCGAAA ATGGAGCGGT CCCAGTTCCG CAGTTGCACA TCGATCAGCC CTTAAAGGCA 
GCCGTTTGGA CTTACTGGCA GCCGAACAAC TCCACCAATC AAACACTGGC CCTCGCCAAA 
CTGTGCCCAA TCTTCGGGCT GCCAATTTAT ATGGGTGCTC AGGCCAGCAA TAGTCTCTGG 
AACTCTATAA TTCAGAAGCA CAACGCTGAG GGTCAAATTG TAGTCAACGA TGGCTTGAGC 
ATTCCCGAGG ACGATATGCG CATGCTTATA CATCTGTTGC ATCAGCGCAA GACCACCTTA 
ACCGATGGCG CTCAGCATAT GCTGCAAAAG TATTATGTGA TCTCCAGGAA AGAGCGACCG 
AATGTCTTCT CTAGCAAGAC CTATATAGTG CTGAAGCAAT TAGCTGAGTG CTTTGCCAAG 
CTGGCGCTGC GGCTGGAGGT CCTTGAATCG GACGTTTGTG TGGCCATCTT TCACTGCGAG 
CATTTCGTGC AGGGCATTTT CGGGGCCAAA GAAAACCAGG CACCGCCGGC GGTCTTCAAT 
TTCAATGTGA TCTCCTGCAT CGATCCGTAC ATGAACAAGT TTACACGCTG GTTACTTCAG 
TATCTGAATA GCTACGAGGA CGAGGAGCTG GGGATGCACG CGGATAAGAG GCGGCGCACA 
GATAGCTGGG ACATGCCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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mei-218 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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