seq cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	seq
Entrez Gene ID	3772396
Full Name	sequoia
Synonyms	2R5,49Fc,CG17724b,CG32904,CG4037,CG4055,Dmel\CG32904,Seq,l(2)49Fc,l(2)vr5,vr5
General protein information	Preferred Names sequoia Names CG32904 gene product from transcript CG32904-RB CG32904-PA CG32904-PB lethal(2)49Fc seq-PA seq-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 49F4-49F7

mRNA and Protein(s)

mRNA	Protein	Name
NM_001032244.2	NP_001027415.1	sequoia, isoform A
NM_001299450.1	NP_001286379.1	sequoia, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study reported heterozygous mutations in sequoia (seq) dominantly exacerbated the trio mutant phenotype, and this seq-enhanced trio mutant genotype blocked the transition of third instar larvae from foragers to wanderers, a requisite behavioral transition prior to pupation.
Title: The function of Drosophila larval class IV dendritic arborization sensory neurons in the larval-pupal transition is separable from their function in mechanical nociception responses.

The findings suggest that seq may act as a context-dependent regulator of Notch signaling, and underscore the growing connection between Seq, Ttk, the PRDM family and Notch signaling.
Title: sequoia controls the type I>0 daughter proliferation switch in the developing Drosophila nervous system.

allele of sequoia dominantly enhances a trio mutant phenotype to influence Drosophila larval behavior
Title: An allele of sequoia dominantly enhances a trio mutant phenotype to influence Drosophila larval behavior.

The seq mutant phenotype arises, in part, owing to the loss of several crucial transcription factors known to be important in peripheral nervous system development such as D-Pax2, Prospero and Hamlet.
Title: Sequoia regulates cell fate decisions in the external sensory organs of adult Drosophila.

The following seq gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the seq cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa11863	NM_001032244.2 Latest version!	Drosophila melanogaster sequoia (seq), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa22392	NM_001299450.1 Latest version!	Drosophila melanogaster sequoia (seq), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

seq ( NM_001032244.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001027415.1 Drosophila melanogaster sequoia (seq), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa11863
Clone ID Related Accession (Same CDS sequence)	NM_001032244.2
Accession Version	NM_001032244.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2649bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	sequoia, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001032244.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAACAAT CGCGTAAATT TGAGGGCAAG ATACCAAAAC TAAGTCAAAC GAGTGCAGCA 
ACAGGAGGAG CAACGGCAGC CGCAGAAGCA TCCACATCCA AGTCTAGTCA AGGGCAGCAG 
CAACAGATTG TCTACGTCAA CATGTTGCCC GCGGCCAATA CCCTGAATGG CATTCCACAG 
CAGCAGCAGC AGCAACAATA TGCGAGCACG GATGGAAATG TCTATCAGCT GCAAAATGAA 
ATACTCTGTG ATGCAAATGG ACATGCCGTG ACTGCCGCCA CGGCCTCATA TCAAACTACG 
GCCAGTAGTC CGCAGCAGCA GCAACAGCAG CAGCAGCAGC AACAGAATGT TGCAACTAGT 
GTTGCCGACA ATGTGGTCAC AACTATTAGT TCGTCCTCCA TCCTGCACAC GAACGCCAAC 
ACCAACGGAG TGGACACATT GGCCGCCACA CAGCAACAAC AACAACAACA GCAGCAGCAG 
CAGCAGCAAC AACAGCAACA GCACTACCAC TATATCACCA CCACCGGCGA GGATGGACAA 
ACCCCGACCA CCATAGTGTT CGAGAACTAC AACGGCGGAA TGCCAGCGGT CAGTTCTGCC 
GCACCAACGC CCGTTCCCGT GTCGCAGGCC AACGCACCCG CGACCACGAC CACCACTGCC 
CTGGTCAACA CGGACGGCAC GATCATCGAG TTCGACGGGA GCACGTACGA GGAGTATCAT 
GTCCTCAAAA GCGAGCCCGG CAGCAGCGAT TGCCTGAGCA ACGGCAGCAG TGCCGTGGCC 
GCCGACATAA TCAAGTACGA TCCCCAGCAC GGTGTCGTCG GCGACGAGGA GGACGACGAT 
GAGAACGGCA TGCTGCATGT GAAGTACGAG GAGCAGAGCC TCGAACACGA TCCGGAAAAC 
GAGCACGATG CCGAGCAGGA GCATGAGTAC GAGGAGTACC AGGTGATCAA GGCCGAGGTG 
GAGGCCGAGG CGGCGGAGCT GGCAGCCGGC ACCACCAGCT ATACGATCAG CCAGGAGCAG 
ACGGGTTCAG GCAACACGGT GCTGTCCTCA ATGGTGCCCA AATCGGGATC AGCGGCGGCC 
GCCAAGCAGA AGCGCAAACG CAAGACGCGC GTAATCGCCG ACGACGAGCA GGGCGAGTAC 
CTGGAAAAGA TGAGTGTACG TGGCCTGGAC ATTGCCCGCT ACGAGCACAT CATCGACGGC 
GTGGCTTACT GCCTGGTCTG CGCCAAGAAC GATATCTTCA AGACGTTTAA GAACAAGTAT 
AGCTTCCAGA GACACGCCTA CCTCTTTCAC GAGGGCCAAA ACCGCAAGAT ATTCGCCTGC 
CCCATTTGCA ACAAGGAGTT CTCGCGTCCG GACAAAATGA AGATGCATAA AAAGGACAAG 
CACGGCGATG TTCCCATGCC ACCATCGGCC ACCACGACGC CGCTAAAGGC TGACGGTCCG 
CCGGCTAAGC GGGCGCGTAC CGCTCGTACT CCGCGCGTCC GCAAGACGAA GGATGGCAGC 
ACGCCGACCA AGAAGCGGTT AGCGCAGATC GACAGTGTTC TGGACGACGT GCAAAATGGG 
GAGTCTCTGA CCATGGCGCC AGTCAGCGTG AGTCACTCCC AGCAACAGCA GCAGCAACAG 
ATCCAGCACA GCATCACAAC GCTGGCTCCC TCGACGCCAT CGCAGCCGCA GCATCAGCTG 
CAGACGCTGC CATCGCTGGA CTTCAGCGGC ATGATCCTGC CTGGAGGCGC ACAGCTGGCA 
CTGGCCAATC CGGGCGCCAC CAGCTCCGTG GGACTGCAAC GAGGACCAGC CACGCCGAAT 
GGTCAGCCGG CGCCGCCCAC CACCACGTTG ATCTACTCGA ATCAACTGGA ACTGCAGCAG 
GCGCTGCTGC AACAGCAGCT GCAAGGTCAG AGCATCATGA TCCAGGATCA AGCCGGCAAC 
CTGGTGCCCC TGCAGCTGGA CGGAACCCAG GCGATATACA CGACGGCGCC GCAGGCTCAG 
CGCCAACAGA CCACGGCCAC ATATCAGACG ACGCAGCAGC AGCAACAGCA GCCAGCGCAG 
CAGCCCGCCG GCCAGGCGCA ACAGCAGCCT CACTACGAGT TGGACAACGT TACCTATTTG 
AGCTCCACGG CGGCGGCCAC ACCGACATCG GCCGAGCAGC AGCAGCAACA ACAGCAGCAG 
CAGCACGTGA TCGGCACGGT TCAGAGCTAT CAGATCCTGA CGCCCGACGG CCTCCAGAAC 
TTCCAACCCA AGCTGGAGAC GGCCGAGCTG GGCGACCTGT GTCCCTATTC GCAGTACACC 
TTCACGACGC ACGCCGGAGC GCAGCCCACG CTGAATGCGA ACGCGCTGGA CGCGCAGACC 
CAGCATCAGC AGCACCACCA GCAGCAGCAG CACCATCACC AGCAGCAGCA ACACCACCAG 
CAGACGCAAC TACTGCAGCA GCAGCCCTTC GCCACGCATC ACAATCCGCA CCAGCAGTTC 
ATGGAGCTGA AGAACGAGCT GCTGATTAAG GGCGGCGACG CCTACACCCT GGACACTGCC 
TCCATGTACC ACCTGCCCTT CTCGCCCACC TCGATGATCA ATGCGGTGAA CGATGTGAAC 
ACCTCGTCGC TGCTGGAAAC CAAAGAAATT AGCTACGATA TATCAGAGGC AGTGCTATTA 
GCTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001027415.1
CDS	559..3207
Translation	MQQSRKFEGKIPKLSQTSAATGGATAAAEASTSKSSQGQQQQIVYVNMLPAANTLNGIPQQQQQQQYASTDGNVYQLQNEILCDANGHAVTAATASYQTTASSPQQQQQQQQQQQNVATSVADNVVTTISSSSILHTNANTNGVDTLAATQQQQQQQQQQQQQQQQQHYHYITTTGEDGQTPTTIVFENYNGGMPAVSSAAPTPVPVSQANAPATTTTTALVNTDGTIIEFDGSTYEEYHVLKSEPGSSDCLSNGSSAVAADIIKYDPQHGVVGDEEDDDENGMLHVKYEEQSLEHDPENEHDAEQEHEYEEYQVIKAEVEAEAAELAAGTTSYTISQEQTGSGNTVLSSMVPKSGSAAAAKQKRKRKTRVIADDEQGEYLEKMSVRGLDIARYEHIIDGVAYCLVCAKNDIFKTFKNKYSFQRHAYLFHEGQNRKIFACPICNKEFSRPDKMKMHKKDKHGDVPMPPSATTTPLKADGPPAKRARTARTPRVRKTKDGSTPTKKRLAQIDSVLDDVQNGESLTMAPVSVSHSQQQQQQQIQHSITTLAPSTPSQPQHQLQTLPSLDFSGMILPGGAQLALANPGATSSVGLQRGPATPNGQPAPPTTTLIYSNQLELQQALLQQQLQGQSIMIQDQAGNLVPLQLDGTQAIYTTAPQAQRQQTTATYQTTQQQQQQPAQQPAGQAQQQPHYELDNVTYLSSTAAATPTSAEQQQQQQQQQHVIGTVQSYQILTPDGLQNFQPKLETAELGDLCPYSQYTFTTHAGAQPTLNANALDAQTQHQQHHQQQQHHHQQQQHHQQTQLLQQQPFATHHNPHQQFMELKNELLIKGGDAYTLDTASMYHLPFSPTSMINAVNDVNTSSLLETKEISYDISEAVLLAL

Target ORF information:

RefSeq Version	NM_001032244.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster sequoia (seq), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001032244.2

ORF Insert Sequence:

ATGCAACAAT CGCGTAAATT TGAGGGCAAG ATACCAAAAC TAAGTCAAAC GAGTGCAGCA 
ACAGGAGGAG CAACGGCAGC CGCAGAAGCA TCCACATCCA AGTCTAGTCA AGGGCAGCAG 
CAACAGATTG TCTACGTCAA CATGTTGCCC GCGGCCAATA CCCTGAATGG CATTCCACAG 
CAGCAGCAGC AGCAACAATA TGCGAGCACG GATGGAAATG TCTATCAGCT GCAAAATGAA 
ATACTCTGTG ATGCAAATGG ACATGCCGTG ACTGCCGCCA CGGCCTCATA TCAAACTACG 
GCCAGTAGTC CGCAGCAGCA GCAACAGCAG CAGCAGCAGC AACAGAATGT TGCAACTAGT 
GTTGCCGACA ATGTGGTCAC AACTATTAGT TCGTCCTCCA TCCTGCACAC GAACGCCAAC 
ACCAACGGAG TGGACACATT GGCCGCCACA CAGCAACAAC AACAACAACA GCAGCAGCAG 
CAGCAGCAAC AACAGCAACA GCACTACCAC TATATCACCA CCACCGGCGA GGATGGACAA 
ACCCCGACCA CCATAGTGTT CGAGAACTAC AACGGCGGAA TGCCAGCGGT CAGTTCTGCC 
GCACCAACGC CCGTTCCCGT GTCGCAGGCC AACGCACCCG CGACCACGAC CACCACTGCC 
CTGGTCAACA CGGACGGCAC GATCATCGAG TTCGACGGGA GCACGTACGA GGAGTATCAT 
GTCCTCAAAA GCGAGCCCGG CAGCAGCGAT TGCCTGAGCA ACGGCAGCAG TGCCGTGGCC 
GCCGACATAA TCAAGTACGA TCCCCAGCAC GGTGTCGTCG GCGACGAGGA GGACGACGAT 
GAGAACGGCA TGCTGCATGT GAAGTACGAG GAGCAGAGCC TCGAACACGA TCCGGAAAAC 
GAGCACGATG CCGAGCAGGA GCATGAGTAC GAGGAGTACC AGGTGATCAA GGCCGAGGTG 
GAGGCCGAGG CGGCGGAGCT GGCAGCCGGC ACCACCAGCT ATACGATCAG CCAGGAGCAG 
ACGGGTTCAG GCAACACGGT GCTGTCCTCA ATGGTGCCCA AATCGGGATC AGCGGCGGCC 
GCCAAGCAGA AGCGCAAACG CAAGACGCGC GTAATCGCCG ACGACGAGCA GGGCGAGTAC 
CTGGAAAAGA TGAGTGTACG TGGCCTGGAC ATTGCCCGCT ACGAGCACAT CATCGACGGC 
GTGGCTTACT GCCTGGTCTG CGCCAAGAAC GATATCTTCA AGACGTTTAA GAACAAGTAT 
AGCTTCCAGA GACACGCCTA CCTCTTTCAC GAGGGCCAAA ACCGCAAGAT ATTCGCCTGC 
CCCATTTGCA ACAAGGAGTT CTCGCGTCCG GACAAAATGA AGATGCATAA AAAGGACAAG 
CACGGCGATG TTCCCATGCC ACCATCGGCC ACCACGACGC CGCTAAAGGC TGACGGTCCG 
CCGGCTAAGC GGGCGCGTAC CGCTCGTACT CCGCGCGTCC GCAAGACGAA GGATGGCAGC 
ACGCCGACCA AGAAGCGGTT AGCGCAGATC GACAGTGTTC TGGACGACGT GCAAAATGGG 
GAGTCTCTGA CCATGGCGCC AGTCAGCGTG AGTCACTCCC AGCAACAGCA GCAGCAACAG 
ATCCAGCACA GCATCACAAC GCTGGCTCCC TCGACGCCAT CGCAGCCGCA GCATCAGCTG 
CAGACGCTGC CATCGCTGGA CTTCAGCGGC ATGATCCTGC CTGGAGGCGC ACAGCTGGCA 
CTGGCCAATC CGGGCGCCAC CAGCTCCGTG GGACTGCAAC GAGGACCAGC CACGCCGAAT 
GGTCAGCCGG CGCCGCCCAC CACCACGTTG ATCTACTCGA ATCAACTGGA ACTGCAGCAG 
GCGCTGCTGC AACAGCAGCT GCAAGGTCAG AGCATCATGA TCCAGGATCA AGCCGGCAAC 
CTGGTGCCCC TGCAGCTGGA CGGAACCCAG GCGATATACA CGACGGCGCC GCAGGCTCAG 
CGCCAACAGA CCACGGCCAC ATATCAGACG ACGCAGCAGC AGCAACAGCA GCCAGCGCAG 
CAGCCCGCCG GCCAGGCGCA ACAGCAGCCT CACTACGAGT TGGACAACGT TACCTATTTG 
AGCTCCACGG CGGCGGCCAC ACCGACATCG GCCGAGCAGC AGCAGCAACA ACAGCAGCAG 
CAGCACGTGA TCGGCACGGT TCAGAGCTAT CAGATCCTGA CGCCCGACGG CCTCCAGAAC 
TTCCAACCCA AGCTGGAGAC GGCCGAGCTG GGCGACCTGT GTCCCTATTC GCAGTACACC 
TTCACGACGC ACGCCGGAGC GCAGCCCACG CTGAATGCGA ACGCGCTGGA CGCGCAGACC 
CAGCATCAGC AGCACCACCA GCAGCAGCAG CACCATCACC AGCAGCAGCA ACACCACCAG 
CAGACGCAAC TACTGCAGCA GCAGCCCTTC GCCACGCATC ACAATCCGCA CCAGCAGTTC 
ATGGAGCTGA AGAACGAGCT GCTGATTAAG GGCGGCGACG CCTACACCCT GGACACTGCC 
TCCATGTACC ACCTGCCCTT CTCGCCCACC TCGATGATCA ATGCGGTGAA CGATGTGAAC 
ACCTCGTCGC TGCTGGAAAC CAAAGAAATT AGCTACGATA TATCAGAGGC AGTGCTATTA 
GCTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

seq ( NM_001299450.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286379.1 Drosophila melanogaster sequoia (seq), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa22392
Clone ID Related Accession (Same CDS sequence)	NM_001299450.1
Accession Version	NM_001299450.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2622bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	sequoia, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAACAAT CGCGTAAATT TGAGGGCAAG ATACCAAAAC TAAGTCAAAC GAGTGCAGCA 
ACAGGAGGAG CAACGGCAGC CGCAGAAGCA TCCACATCCA AGTCTAGTCA AGGGCAGCAG 
CAACAGATTG TCTACGTCAA CATGTTGCCC GCGGCCAATA CCCTGAATGG CATTCCACAG 
CAGCAGCAGC AGCAACAATA TGCGAGCACG GATGGAAATG TCTATCAGCT GCAAAATGAA 
ATACTCTGTG ATGCAAATGG ACATGCCGTG ACTGCCGCCA CGGCCTCATA TCAAACTACG 
GCCAGTAGTC CGCAGCAGCA GCAACAGCAG CAGCAGCAGC AACAGAATGT TGCAACTAGT 
GTTGCCGACA ATGTGGTCAC AACTATTAGT TCGTCCTCCA TCCTGCACAC GAACGCCAAC 
ACCAACGGAG TGGACACATT GGCCGCCACA CAGCAACAAC AACAACAACA GCAGCAGCAG 
CAGCAGCAAC AACAGCAACA GCACTACCAC TATATCACCA CCACCGGCGA GGATGGACAA 
ACCCCGACCA CCATAGTGTT CGAGAACTAC AACGGCGGAA TGCCAGCGGT CAGTTCTGCC 
GCACCAACGC CCGTTCCCGT GTCGCAGGCC AACGCACCCG CGACCACGAC CACCACTGCC 
CTGGTCAACA CGGACGGCAC GATCATCGAG TTCGACGGGA GCACGTACGA GGAGTATCAT 
GTCCTCAAAA GCGAGCCCGG CAGCAGCGAT TGCCTGAGCA ACGGCAGCAG TGCCGTGGCC 
GCCGACATAA TCAAGTACGA TCCCCAGCAC GGTGTCGTCG GCGACGAGGA GGACGACGAT 
GAGAACGGCA TGCTGCATGT GAAGTACGAG GAGCAGAGCC TCGAACACGA TCCGGAAAAC 
GAGCACGATG CCGAGCAGGA GCATGAGTAC GAGGAGTACC AGGTGATCAA GGCCGAGGTG 
GAGGCCGAGG CGGCGGAGCT GGCAGCCGGC ACCACCAGCT ATACGATCAG CCAGGAGCAG 
ACGGGTTCAG GCAACACGGT GCTGTCCTCA ATGGTGCCCA AATCGGGATC AGCGGCGGCC 
GCCAAGCAGA AGCGCAAACG CAAGACGCGC GTAATCGCCG ACGACGAGCA GGGCGAGTAC 
CTGGAAAAGA TGAGTGTACG TGGCCTGGAC ATTGCCCGCT ACGAGCACAT CATCGACGGC 
GTGGCTTACT GCCTGGTCTG CGCCAAGAAC GATATCTTCA AGACGTTTAA GAACAAGTAT 
AGCTTCCAGA GACACGCCTA CCTCTTTCAC GAGGGCCAAA ACCGCAAGAT ATTCGCCTGC 
CCCATTTGCA ACAAGGAGTT CTCGCGTCCG GACAAAATGA AGATGCATAA AAAGGACAAG 
CACGGCGATG TTCCCATGCC ACCATCGGCC ACCACGACGC CGCTAAAGGC TGACGGTCCG 
CCGGCTAAGC GGGCGCGTAC CGCTCGTACT CCGCGCGTCC GCAAGACGAA GGATGGCAGC 
ACGCCGACCA AGAAGCGGTT AGCGCAGATC GACAGTGTTC TGGACGACGT GCAAAATGGG 
GAGTCTCTGA CCATGGCGCC AGTCAGCGTG AGTCACTCCC AGCAACAGCA GCAGCAACAG 
ATCCAGCACA GCATCACAAC GCTGGCTCCC TCGACGCCAT CGCAGCCGCA GCATCAGCTG 
CAGACGCTGC CATCGCTGGA CTTCAGCGGC ATGATCCTGC CTGGAGGCGC ACAGCTGGCA 
CTGGCCAATC CGGGCGCCAC CAGCTCCGTG GGACTGCAAC GAGGACCAGC CACGCCGAAT 
GGTCAGCCGG CGCCGCCCAC CACCACGTTG ATCTACTCGA ATCAACTGGA ACTGCAGCAG 
GCGCTGCTGC AACAGCAGCT GCAAGGTCAG AGCATCATGA TCCAGGATCA AGCCGGCAAC 
CTGGTGCCCC TGCAGCTGGA CGGAACCCAG GCGATATACA CGACGGCGCC GCAGGCTCAG 
CGCCAACAGA CCACGGCCAC ATATCAGACG ACGCAGCAGC AGCAACAGCA GCCAGCGCAG 
CAGCCCGCCG GCCAGGCGCA ACAGCAGCCT CACTACGAGT TGGACAACGT TACCTATTTG 
AGCTCCACGG CGGCGGCCAC ACCGACATCG GCCGAGCAGC AGCAGCAACA ACAGCAGCAG 
CAGCACGTGA TCGGCACGGT TCAGAGCTAT CAGATCCTGA CGCCCGACGG CCTCCAGAAC 
TTCCAACCCA AGCTGGAGAC GGCCGAGCTG GGCGACCTGT GTCCCTATTC GCAGTACACC 
TTCACGACGC ACGCCGGAGC GCAGCCCACG CTGAATGCGA ACGCGCTGGA CGCGCAGACC 
CAGCATCAGC AGCACCACCA GCAGCAGCAG CACCATCACC AGCAGCAGCA ACACCACCAG 
CAGACGCAAC TACTGCAGCA GCAGCCCTTC GCCACGCATC ACAATCCGCA CCAGCAGTTC 
ATGGAGCTGA AGAACGAGCT GCTGATTAAG GGCGGCGACG CCTACACCCT GGACACTGCC 
TCCATGTACC ACCTGCCCTT CTCGCCCACC TCGATGATCA ATGCGGTGAA CGATGTGAAC 
ACCTCGTCGC TGCTGGAAAC CAAAGAAATT AGGTTCTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286379.1
CDS	559..3180
Translation	MQQSRKFEGKIPKLSQTSAATGGATAAAEASTSKSSQGQQQQIVYVNMLPAANTLNGIPQQQQQQQYASTDGNVYQLQNEILCDANGHAVTAATASYQTTASSPQQQQQQQQQQQNVATSVADNVVTTISSSSILHTNANTNGVDTLAATQQQQQQQQQQQQQQQQQHYHYITTTGEDGQTPTTIVFENYNGGMPAVSSAAPTPVPVSQANAPATTTTTALVNTDGTIIEFDGSTYEEYHVLKSEPGSSDCLSNGSSAVAADIIKYDPQHGVVGDEEDDDENGMLHVKYEEQSLEHDPENEHDAEQEHEYEEYQVIKAEVEAEAAELAAGTTSYTISQEQTGSGNTVLSSMVPKSGSAAAAKQKRKRKTRVIADDEQGEYLEKMSVRGLDIARYEHIIDGVAYCLVCAKNDIFKTFKNKYSFQRHAYLFHEGQNRKIFACPICNKEFSRPDKMKMHKKDKHGDVPMPPSATTTPLKADGPPAKRARTARTPRVRKTKDGSTPTKKRLAQIDSVLDDVQNGESLTMAPVSVSHSQQQQQQQIQHSITTLAPSTPSQPQHQLQTLPSLDFSGMILPGGAQLALANPGATSSVGLQRGPATPNGQPAPPTTTLIYSNQLELQQALLQQQLQGQSIMIQDQAGNLVPLQLDGTQAIYTTAPQAQRQQTTATYQTTQQQQQQPAQQPAGQAQQQPHYELDNVTYLSSTAAATPTSAEQQQQQQQQQHVIGTVQSYQILTPDGLQNFQPKLETAELGDLCPYSQYTFTTHAGAQPTLNANALDAQTQHQQHHQQQQHHHQQQQHHQQTQLLQQQPFATHHNPHQQFMELKNELLIKGGDAYTLDTASMYHLPFSPTSMINAVNDVNTSSLLETKEIRFF

Target ORF information:

RefSeq Version	NM_001299450.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster sequoia (seq), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299450.1

ORF Insert Sequence:

ATGCAACAAT CGCGTAAATT TGAGGGCAAG ATACCAAAAC TAAGTCAAAC GAGTGCAGCA 
ACAGGAGGAG CAACGGCAGC CGCAGAAGCA TCCACATCCA AGTCTAGTCA AGGGCAGCAG 
CAACAGATTG TCTACGTCAA CATGTTGCCC GCGGCCAATA CCCTGAATGG CATTCCACAG 
CAGCAGCAGC AGCAACAATA TGCGAGCACG GATGGAAATG TCTATCAGCT GCAAAATGAA 
ATACTCTGTG ATGCAAATGG ACATGCCGTG ACTGCCGCCA CGGCCTCATA TCAAACTACG 
GCCAGTAGTC CGCAGCAGCA GCAACAGCAG CAGCAGCAGC AACAGAATGT TGCAACTAGT 
GTTGCCGACA ATGTGGTCAC AACTATTAGT TCGTCCTCCA TCCTGCACAC GAACGCCAAC 
ACCAACGGAG TGGACACATT GGCCGCCACA CAGCAACAAC AACAACAACA GCAGCAGCAG 
CAGCAGCAAC AACAGCAACA GCACTACCAC TATATCACCA CCACCGGCGA GGATGGACAA 
ACCCCGACCA CCATAGTGTT CGAGAACTAC AACGGCGGAA TGCCAGCGGT CAGTTCTGCC 
GCACCAACGC CCGTTCCCGT GTCGCAGGCC AACGCACCCG CGACCACGAC CACCACTGCC 
CTGGTCAACA CGGACGGCAC GATCATCGAG TTCGACGGGA GCACGTACGA GGAGTATCAT 
GTCCTCAAAA GCGAGCCCGG CAGCAGCGAT TGCCTGAGCA ACGGCAGCAG TGCCGTGGCC 
GCCGACATAA TCAAGTACGA TCCCCAGCAC GGTGTCGTCG GCGACGAGGA GGACGACGAT 
GAGAACGGCA TGCTGCATGT GAAGTACGAG GAGCAGAGCC TCGAACACGA TCCGGAAAAC 
GAGCACGATG CCGAGCAGGA GCATGAGTAC GAGGAGTACC AGGTGATCAA GGCCGAGGTG 
GAGGCCGAGG CGGCGGAGCT GGCAGCCGGC ACCACCAGCT ATACGATCAG CCAGGAGCAG 
ACGGGTTCAG GCAACACGGT GCTGTCCTCA ATGGTGCCCA AATCGGGATC AGCGGCGGCC 
GCCAAGCAGA AGCGCAAACG CAAGACGCGC GTAATCGCCG ACGACGAGCA GGGCGAGTAC 
CTGGAAAAGA TGAGTGTACG TGGCCTGGAC ATTGCCCGCT ACGAGCACAT CATCGACGGC 
GTGGCTTACT GCCTGGTCTG CGCCAAGAAC GATATCTTCA AGACGTTTAA GAACAAGTAT 
AGCTTCCAGA GACACGCCTA CCTCTTTCAC GAGGGCCAAA ACCGCAAGAT ATTCGCCTGC 
CCCATTTGCA ACAAGGAGTT CTCGCGTCCG GACAAAATGA AGATGCATAA AAAGGACAAG 
CACGGCGATG TTCCCATGCC ACCATCGGCC ACCACGACGC CGCTAAAGGC TGACGGTCCG 
CCGGCTAAGC GGGCGCGTAC CGCTCGTACT CCGCGCGTCC GCAAGACGAA GGATGGCAGC 
ACGCCGACCA AGAAGCGGTT AGCGCAGATC GACAGTGTTC TGGACGACGT GCAAAATGGG 
GAGTCTCTGA CCATGGCGCC AGTCAGCGTG AGTCACTCCC AGCAACAGCA GCAGCAACAG 
ATCCAGCACA GCATCACAAC GCTGGCTCCC TCGACGCCAT CGCAGCCGCA GCATCAGCTG 
CAGACGCTGC CATCGCTGGA CTTCAGCGGC ATGATCCTGC CTGGAGGCGC ACAGCTGGCA 
CTGGCCAATC CGGGCGCCAC CAGCTCCGTG GGACTGCAAC GAGGACCAGC CACGCCGAAT 
GGTCAGCCGG CGCCGCCCAC CACCACGTTG ATCTACTCGA ATCAACTGGA ACTGCAGCAG 
GCGCTGCTGC AACAGCAGCT GCAAGGTCAG AGCATCATGA TCCAGGATCA AGCCGGCAAC 
CTGGTGCCCC TGCAGCTGGA CGGAACCCAG GCGATATACA CGACGGCGCC GCAGGCTCAG 
CGCCAACAGA CCACGGCCAC ATATCAGACG ACGCAGCAGC AGCAACAGCA GCCAGCGCAG 
CAGCCCGCCG GCCAGGCGCA ACAGCAGCCT CACTACGAGT TGGACAACGT TACCTATTTG 
AGCTCCACGG CGGCGGCCAC ACCGACATCG GCCGAGCAGC AGCAGCAACA ACAGCAGCAG 
CAGCACGTGA TCGGCACGGT TCAGAGCTAT CAGATCCTGA CGCCCGACGG CCTCCAGAAC 
TTCCAACCCA AGCTGGAGAC GGCCGAGCTG GGCGACCTGT GTCCCTATTC GCAGTACACC 
TTCACGACGC ACGCCGGAGC GCAGCCCACG CTGAATGCGA ACGCGCTGGA CGCGCAGACC 
CAGCATCAGC AGCACCACCA GCAGCAGCAG CACCATCACC AGCAGCAGCA ACACCACCAG 
CAGACGCAAC TACTGCAGCA GCAGCCCTTC GCCACGCATC ACAATCCGCA CCAGCAGTTC 
ATGGAGCTGA AGAACGAGCT GCTGATTAAG GGCGGCGACG CCTACACCCT GGACACTGCC 
TCCATGTACC ACCTGCCCTT CTCGCCCACC TCGATGATCA ATGCGGTGAA CGATGTGAAC 
ACCTCGTCGC TGCTGGAAAC CAAAGAAATT AGGTTCTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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seq cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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