eys cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following eys gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eys cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa22383 NM_001272995.1
Latest version!
Drosophila melanogaster eyes shut (eys), transcript variant E, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OFa22383 NM_001032399.3
Latest version!
Drosophila melanogaster eyes shut (eys), transcript variant F, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa22383
    Clone ID Related Accession (Same CDS sequence) NM_001272995.1 , NM_001032399.3
    Accession Version NM_001272995.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6531bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product eyes shut, isoform E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGAGCAACG TGCATCAGTT TGATACGCAG ACGATGGCGG AATCGCCTCA AATTAGACGC 
    GACATGGGTC GCCTATGTGC CACGTGGCCT TCGAAGGACT CTGAGGACGG GGCCGGCACA
    GCTTTGAGGG CAGCCACACC TCTGACAGCC AACGGAGCCA CCACCACCGG GCTGAGTGTT
    ACCCTTGCCC CGAAGGACAT GCAACGAAAC CACCTGCTAA AGATGCCAAC AGCAACAATA
    GAGAAACCAA CGATAACAGC CACCATCGCC AGCAGCAGCA GCACCAGCAC TAGCACCACT
    AGAAAATCAG TTACAGCCAC ACGGAGCCTG AAGTTGAACC CGAACATTCT CCTTCCGACA
    CTAAGGATAT TAGCAAGGGG TCTGCTGCTA CCCGCTCTGA TCCTGGCCAT CCTCGTGGGC
    AGCAGCCAGG CGGGATTTGC CTGCCTCAGC AATCCCTGTG TGTTCGGCGT GTGCATCGAT
    GGCCTAAACA GCTCGTACTC GTGTTACTGC ATTGATGGCT ACACAGGGAT CCAGTGCCAA
    ACGAATTGGG ACGAGTGCTG GTCGAGTCCT TGCCAGAACG GCGGGACATG TGTGGACGGT
    GTGGCCTACT ACAATTGTAC GTGTCCGGAA GGATTTTCTG GATCCAACTG CGAGGAGAAC
    GTCGACGAAT GCATGTCGAA TCCCTGCCAG AACGGCGGAC TCTGCCGGGA CAGAACCAAT
    GGCTACATAT GCACCTGCCA ACCAGGTTAC CTGGGATCTC ACTGCGAACT GGATGTCGCT
    GTCTGCGAGA CAGGAACTGG TGCTCGTTGC CAGCACGGTG GTGAGTGCAT CGAGGGTCCT
    GGACTGGAGT TCACCTGCGA CTGTCCGGCG GGCTGGCATG GTCGGATTTG CCAGGAGGAG
    ATTAACGAGT GCGCCTCGTC GCCCTGCCAA AATGGAGGCG TCTGCGTGGA CAAGCTGGCG
    GCCTATGCCT GCGCCTGTCC CATGGGATAT ACGGGCATCA ACTGCGAGGA GGAGATCCTC
    ATCTGTGCGG ACAATCCGTG TCAGAATAAT GCCTTGTGCT TGATGGAGGA GGGTGTACCC
    ACGTGCTATT GTGTACCCGA CTATCATGGC GAGAAGTGTG AGTTCCAGTA CGACGAATGC
    CAATTGGGAC CGCGGTGCAT GAATGGTGGT GTTTGCATCG ACGGTGTCGA TACCTTCTCC
    TGCTCATGTC CTCCCCTATT GACCGGTATG CTCTGCGAGT GCCTGATGGT GGGTGAGGAG
    TCCCTGGACT GCAACTACAC AGCACCGGCC ACGCAATCTC CGCCACGGAG AACTACCACC
    ACATCCACAA TGGCTCCACC CACAGTGAGG CCCGTCACTC CGCCGGAGAC AACGGTATCG
    CCATCCAGAG CTTCAGAAGA GGTGGAGATT ATAGTAGTTA CCACATCTGC TCCTGCGGAA
    GTGGTCACCT CTGTGCTATC CCCATCATCC TCTTCTTCAT CGTCGGAGGA AGGCGTTTCC
    GTCGAGATTA AAACACCGAC TGTGGCGCCC CCAGAAAGCG GTAGCCATAG CATATCCGTG
    GAGCAAACAA CGGCCGTTCC AGCACAACCC GAACCCGAAT CCGAACAAGA ACCAGAATCT
    AAGCCACATC CCGAATCGGA ATCGGCGTCG GAATCGGAAA CGGAAACGGA GGAGGAAATA
    ATTCCGGGAA CTACAGCGCG ACCACCAACA AGCAGATCCT CCAGTTCTTC GGAGGAATCT
    CCCAGCATCT TCACCACTTT GCCTCCGTTG CCGGGAAAGC CTCAAACGTC TGCGTCGTCG
    GAAAGTTCCG GAGAAGTGGT TACCTCCGAG GAATACACCA CTGTGCCGCA CTTCGAAGTG
    AGTGGCAGCA AAAGTGAAAG TGGTAGTGAA GAGGTCACCA CTGTGCGACC CACTGCAGCA
    CCGAGTATCA CCATTTCAGT GGACATCACC TCATCCGGAA GTAGCAGTAG TAGTTCGGAA
    TCAGTGGAGG TATTCACCAC ACCAGCTCCC GTTTTCGTGC AGAGAGTGAC TACGATCGAA
    ACTAGCATCT CCATAGACTA TGTCACACCC ACTCCTCTGC CGGAAACCAC CACCCCACGG
    GTGGTCCCGG TGCCCAGGCC TACCTTTGCC CCAGAGCCAC CACTGGATGT GGTGGAAACG
    ACTGCCTCAA CTCACCACCT TTGGACAGAG GTGCCCACCA CGGCGGCTCC CTTCTTCACG
    GAGTATCCGG CGGAAGTGCT GATCACTACA CATCGAACGA GTGCTGGCAG ATTCACCACA
    GTTCAACCAC CTGCAGGTGT CACCACCACC TCACCCACTG AGGACTCATC CGTTGAGTTA
    CCCACTCCGC ACACGCCCCA AATTGTGGTC ACCATTTTGG ATTCAAATGA AGTTATACCT
    TCGCTGATCA CAACCACTGG ATCCCCGACA ACACACCATC ACCACCATCA TCATCCTCAC
    CATGAAGCCG AAGGAACCAC TCTTCAGCCT CTCGAAGAAG ACGAGCACCA CCATCATCAT
    CATCATGACG AGTTCACCAC TCCACAGCCA GTGGAAATAA CTACGGGACA TCCTTTGCAA
    ACAGAGGACC TTATTGGAGT GCAGGAACCT GCCGTGGTGA CCACAGAGTC GCCCTTTGCT
    CCAGCTGAGA CAACTGTGGT TCCTGTGGTG GTGCCCGCTA CGATTGCACC ACTTGGAACT
    GCAGCACCAC CAGCCACACC AGCTCCTGTT CCTCCAGCGA CTACCACTCC TCCTCCTTCG
    CCTCCTTCCT TGGCTACAGA GACACCTACA CTGCCACCCA CTTTACCTCC TGTTACCCTG
    CCACCGGTAA CCCAGCCGCC GCCCACGATA CCACCCACTC CTCCATCCAC ACAATCCGCA
    CAAACTTTAC CGCCACCGAC GTCGGCCATA AATGTTTACA CCACTCCCGA TGGACCACCC
    ACGGCTTCCC AAACGAAGCC ATCGGTCACG GAGAGCAGCG AGGAAGTGGA GGGCACAAAT
    ACGGTCTCCA CCGGCGGAAG GGGATCCGGC GGAGTGCCCG AGGAGAAGGC AGGCGATGTC
    GATTGCATCA AGCTGGGCTG CTACAACGGT GGCACCTGCG TAACCACATC CGAAGGATCG
    CGGTGTGTTT GTCGTTTCGA CCGCCAAGGA CCTCTTTGCG AGCTGCCCAT TATCATCCGG
    AATGCCGCCT TCTCCGGCGA CTCCTATGTG TCGCACCGCA TCTACAAGGA CATCGGGGGC
    CACGAGTCCT TGGACGCCGT CCTGCCGATG CACATCCAGC TGAAGGTGCG AACCCGGGCC
    ACCAACGGGC TGATCATGCT GGCGGCGGCG CAGGGCACCA AGGGTGGCCA CTACATGGCC
    CTTTTCCTGC AGAAGGGACT CATGCAGTTC CAGTTCTCCT GCGGACTGCA GACGATGCTG
    CTGAGTGAAC TGGAAACGCC GGTCAACACC GGCCACGAAA TCACCATTCG AGCTGAATTG
    GACTTCAGCC GGAATTACAC ACACTGCAAC GCCTCGTTGC TGGTGAACGA CACGTTGGCC
    ATGTCCGGGG ATCAGCCCAC CTGGCTGAAG CTACTACCGC CGCGCCTTCA CACCCCGGAG
    GCCATCCTCA ACACCTGGCT GCATCTGGGC GGAGCGCCCC AGGCGCCGAT TGGCCTGATT
    ATCGAACTGC CGCCGGCCCA AAGTGGAAGC GGCTTCACCG GCTGCCTCCA TACGCTGCGC
    ATCAATGGAC AGGCTCGCGA GATTTTCGGC GATGCCCTCG ATGGCTTTGG CATTACGGAA
    TGCGGCTCAT TGGCGTGTCT ATCGAGTCCT TGCCGCAATG GCGCCGCCTG CATAAAGATC
    GAGACCAACG ATCTGGACGA GAATGGCGAG AAGGCGGAGA AGTGGAAGTG CAAGTGCCCC
    ACCGGCTACA TGGGCCCCAC CTGCGAGATA TCCGTGTGCG AGGACAACCC GTGCCAGTAC
    GGTGGAACCT GCGTACAATT TCCGGGCAGC GGTTACCTCT GCCTGTGTCC GCTGGGCAAG
    CACGGCCACT ATTGCGAGCA CAATCTTGAA GTGGCCCTGC CATCCTTCTC CGGCAGCGTC
    AATGGACTCT CCTCGTTTGT GGCCTACACG GTGCCGATTC CCCTCGAGTA TTCGCTCGAG
    CTGAGCTTCA AAATCCTGCC GCAGACCATG TCTCAGATTT CGCTGCTGGC CTTCTTCGGG
    CAGTCGGGTT ATCACGACGA GAAGAGCGAT CACCTGGCGG TGAGCTTCAT CCAGGGCTAC
    ATAATGCTCA CCTGGAATCT GGGAGCCGGG CCCCGTCGCA TATTCACACA GAAACCCATT
    GATTTCCGGC TGGATGCTCC CCGGGTTCCG TACGAGATTA AGGTGGGCCG GATTGGAAGA
    CAGGCCTGGC TCTCGGTGGA TGGAAAGTTC AATATTACGG GTCGATCGCC CGGAAGTGGC
    AGTCGGATGG ATGTGTTGCC TATTCTGTAT CTCGGCGGTC ACGAGATAGC CAATTTCAAT
    ACGCTGCCAC ACGATTTGCC ACTGCATTCG GGATTCCAGG GATGCATATA CGATGTACAG
    CTGAAGGCGG GACAGGTCAC GGTGCCCCTC CAGGAGACGC GCGGGGTCAG AGGTCGGGGC
    GTGGGTCAAT GTGGAACCAG GGAATGCCAT CGCCATGCCT GCCAGCACGA TGGCGCCTGC
    CTGCAGCATG GAGCAACCTT TACTTGCATC TGCCAGGAGG GCTGGTATGG GCCACTGTGT
    GCCCAGCCCA CGAATCCCTG TGACTCCTTC AACAACAAAT GCTACGAGGA CGCCACCTGT
    GTGCCGCTGG TCAATGGCTA CGAATGCGAT TGTCCTGTAG GACGCACAGG CAAAAACTGC
    GAGGAGGTAA TTCGATCCCT GAGCGACGTA TCCCTGACTG GCAGACGATC CTATCTGGCG
    GTTCGCTGGC CATATCTATA CGATGGCGGG GATAAACTGG GCGCTAAGCG CTCCCAAATG
    GTCAGCTACC GGAACTTCAC CAAGAAACTA ATGCCCCCGA AGCCCATTAC CACGCCCAGC
    AGCCACTTTG TGATGAAACT GCTGAATGAG GTGGAGAAGC AGCGCAGCTT CTCGCCAGTT
    CCCCTGATGG GATCGAAGTC CTTCGAGGAG CACCATCGAG TGCAGTTCTT CTTCATCGAG
    TTCCAACTGC GCCCGCTCTC GGAAAGAGGC CTCCTTCTGT ACTTCGGTAC CCTGAACAAC
    AATCAGGACA AGAAGATAGG ATTCGTGTCG CTATCGCTGC AGGGCGGAGT GGTGGAGTTC
    CGGATTTCCG GGCCCAGCAA TCATGTCACG GTGGTGCGGA GCGTTCGCAT GTTGGCCATC
    GGCGAGTGGC ACAAGATCAA GATGGCGCAG CGCGGAAGAT GGCTGACCCT GTGGGTGGAG
    GGTAGCGCCT CGTCGGCTTT GGCTCCTTCG GCTGAGGTGC TCGTCGAGCC CGACTCACTG
    CTCTACATTG GCGGGCTGAA GGATGTGTCC AAGCTGCCGC ACAACGCCAT CTCCGGTTTC
    CCGATTCCGT TCAGGGGATG TGTCCGGGGT CTGGTGGTCA GTGGAACTCG CATTGTGCTC
    AACGAAACCA ACATTGTGGA ATCGCGCAAC ATTCGAGACT GTGATGGCAC CGCTTGTGGC
    GGAGACTCCT GCGAGTCCGG AGGACATTGC TGGCTGGACG AGAAGCTGCA GCCGCATTGC
    ATCTGTCCGG AGTACGCGAA AGGAGATCGA TGCGAGTACT CGGAGACCTG CAAGCTCATT
    CCGTGCAAAA ACAATGGAAG ATGCCTGAGA AGTGGACGCT GCTCGTGTCC AAATGGATGG
    GGTGGCTTCT ACTGCGAGAT TGCCATGAGC AAGCCGACGA CGCCGAGCTT CCGTGGCAAC
    AGTTACCTGA TCTTGCCGCC GCCGCGAATT CCGATGAAAG ACAAGCGGCG GGGACCCTCC
    CTGTACGTCC GCCCCCGCGA AGCCATCCAA GTCTCGCTGA ACTTCTCCAC CATCGAACCG
    GACGGACTGC TCCTGTGGAG CGAGCACGAG AGGAGCAAGT TCCTCGGCCT GGGCCTGGAG
    GCGGGCCACC TCAAGCTGGC CAGCAACCTC CTGGGCAGCA CCAACGACAC GGTCCGTGCT
    CCGGCCAGCG GATTCATAGC CGACGGAGCC TGGCACTGGA CCAGCGTCCT GCTGGACCGG
    TCCAGGCTGG AGCTGCAGCT GGACGGCGAG GTGATCTTCA CGGAGCGATT GCCCGAGGGC
    GGACGGTCAC TGGGCTCGAC CACGCCGAGA AGCACTCTGG CGGGCAGGCG CAAGAATTCG
    AGCAAGGAGC CAACAATTAG CTACGAGGAT GTCTTCTACT TGGGCGGCTT TCCCAACTCG
    GACTCGGTGA GCAGGCGCAC AAAGGGTCGC TTCTTCGATC CCTTCAAGGG CTGCCTGCAG
    GATATCCAGT TCGGAGCCGA GCCCACAGCG ATTATCAGCG ACTTCTCGAC GTACCAGGGC
    GAGAACATTG GATCCTGTGA TCTGCACGGC GATGAGCCGC TTACTGTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001259924.2
    CDS884..7414
    Translation

    Target ORF information:

    RefSeq Version NM_001272995.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster eyes shut (eys), transcript variant E, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001272995.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGAGCAACG TGCATCAGTT TGATACGCAG ACGATGGCGG AATCGCCTCA AATTAGACGC 
    GACATGGGTC GCCTATGTGC CACGTGGCCT TCGAAGGACT CTGAGGACGG GGCCGGCACA
    GCTTTGAGGG CAGCCACACC TCTGACAGCC AACGGAGCCA CCACCACCGG GCTGAGTGTT
    ACCCTTGCCC CGAAGGACAT GCAACGAAAC CACCTGCTAA AGATGCCAAC AGCAACAATA
    GAGAAACCAA CGATAACAGC CACCATCGCC AGCAGCAGCA GCACCAGCAC TAGCACCACT
    AGAAAATCAG TTACAGCCAC ACGGAGCCTG AAGTTGAACC CGAACATTCT CCTTCCGACA
    CTAAGGATAT TAGCAAGGGG TCTGCTGCTA CCCGCTCTGA TCCTGGCCAT CCTCGTGGGC
    AGCAGCCAGG CGGGATTTGC CTGCCTCAGC AATCCCTGTG TGTTCGGCGT GTGCATCGAT
    GGCCTAAACA GCTCGTACTC GTGTTACTGC ATTGATGGCT ACACAGGGAT CCAGTGCCAA
    ACGAATTGGG ACGAGTGCTG GTCGAGTCCT TGCCAGAACG GCGGGACATG TGTGGACGGT
    GTGGCCTACT ACAATTGTAC GTGTCCGGAA GGATTTTCTG GATCCAACTG CGAGGAGAAC
    GTCGACGAAT GCATGTCGAA TCCCTGCCAG AACGGCGGAC TCTGCCGGGA CAGAACCAAT
    GGCTACATAT GCACCTGCCA ACCAGGTTAC CTGGGATCTC ACTGCGAACT GGATGTCGCT
    GTCTGCGAGA CAGGAACTGG TGCTCGTTGC CAGCACGGTG GTGAGTGCAT CGAGGGTCCT
    GGACTGGAGT TCACCTGCGA CTGTCCGGCG GGCTGGCATG GTCGGATTTG CCAGGAGGAG
    ATTAACGAGT GCGCCTCGTC GCCCTGCCAA AATGGAGGCG TCTGCGTGGA CAAGCTGGCG
    GCCTATGCCT GCGCCTGTCC CATGGGATAT ACGGGCATCA ACTGCGAGGA GGAGATCCTC
    ATCTGTGCGG ACAATCCGTG TCAGAATAAT GCCTTGTGCT TGATGGAGGA GGGTGTACCC
    ACGTGCTATT GTGTACCCGA CTATCATGGC GAGAAGTGTG AGTTCCAGTA CGACGAATGC
    CAATTGGGAC CGCGGTGCAT GAATGGTGGT GTTTGCATCG ACGGTGTCGA TACCTTCTCC
    TGCTCATGTC CTCCCCTATT GACCGGTATG CTCTGCGAGT GCCTGATGGT GGGTGAGGAG
    TCCCTGGACT GCAACTACAC AGCACCGGCC ACGCAATCTC CGCCACGGAG AACTACCACC
    ACATCCACAA TGGCTCCACC CACAGTGAGG CCCGTCACTC CGCCGGAGAC AACGGTATCG
    CCATCCAGAG CTTCAGAAGA GGTGGAGATT ATAGTAGTTA CCACATCTGC TCCTGCGGAA
    GTGGTCACCT CTGTGCTATC CCCATCATCC TCTTCTTCAT CGTCGGAGGA AGGCGTTTCC
    GTCGAGATTA AAACACCGAC TGTGGCGCCC CCAGAAAGCG GTAGCCATAG CATATCCGTG
    GAGCAAACAA CGGCCGTTCC AGCACAACCC GAACCCGAAT CCGAACAAGA ACCAGAATCT
    AAGCCACATC CCGAATCGGA ATCGGCGTCG GAATCGGAAA CGGAAACGGA GGAGGAAATA
    ATTCCGGGAA CTACAGCGCG ACCACCAACA AGCAGATCCT CCAGTTCTTC GGAGGAATCT
    CCCAGCATCT TCACCACTTT GCCTCCGTTG CCGGGAAAGC CTCAAACGTC TGCGTCGTCG
    GAAAGTTCCG GAGAAGTGGT TACCTCCGAG GAATACACCA CTGTGCCGCA CTTCGAAGTG
    AGTGGCAGCA AAAGTGAAAG TGGTAGTGAA GAGGTCACCA CTGTGCGACC CACTGCAGCA
    CCGAGTATCA CCATTTCAGT GGACATCACC TCATCCGGAA GTAGCAGTAG TAGTTCGGAA
    TCAGTGGAGG TATTCACCAC ACCAGCTCCC GTTTTCGTGC AGAGAGTGAC TACGATCGAA
    ACTAGCATCT CCATAGACTA TGTCACACCC ACTCCTCTGC CGGAAACCAC CACCCCACGG
    GTGGTCCCGG TGCCCAGGCC TACCTTTGCC CCAGAGCCAC CACTGGATGT GGTGGAAACG
    ACTGCCTCAA CTCACCACCT TTGGACAGAG GTGCCCACCA CGGCGGCTCC CTTCTTCACG
    GAGTATCCGG CGGAAGTGCT GATCACTACA CATCGAACGA GTGCTGGCAG ATTCACCACA
    GTTCAACCAC CTGCAGGTGT CACCACCACC TCACCCACTG AGGACTCATC CGTTGAGTTA
    CCCACTCCGC ACACGCCCCA AATTGTGGTC ACCATTTTGG ATTCAAATGA AGTTATACCT
    TCGCTGATCA CAACCACTGG ATCCCCGACA ACACACCATC ACCACCATCA TCATCCTCAC
    CATGAAGCCG AAGGAACCAC TCTTCAGCCT CTCGAAGAAG ACGAGCACCA CCATCATCAT
    CATCATGACG AGTTCACCAC TCCACAGCCA GTGGAAATAA CTACGGGACA TCCTTTGCAA
    ACAGAGGACC TTATTGGAGT GCAGGAACCT GCCGTGGTGA CCACAGAGTC GCCCTTTGCT
    CCAGCTGAGA CAACTGTGGT TCCTGTGGTG GTGCCCGCTA CGATTGCACC ACTTGGAACT
    GCAGCACCAC CAGCCACACC AGCTCCTGTT CCTCCAGCGA CTACCACTCC TCCTCCTTCG
    CCTCCTTCCT TGGCTACAGA GACACCTACA CTGCCACCCA CTTTACCTCC TGTTACCCTG
    CCACCGGTAA CCCAGCCGCC GCCCACGATA CCACCCACTC CTCCATCCAC ACAATCCGCA
    CAAACTTTAC CGCCACCGAC GTCGGCCATA AATGTTTACA CCACTCCCGA TGGACCACCC
    ACGGCTTCCC AAACGAAGCC ATCGGTCACG GAGAGCAGCG AGGAAGTGGA GGGCACAAAT
    ACGGTCTCCA CCGGCGGAAG GGGATCCGGC GGAGTGCCCG AGGAGAAGGC AGGCGATGTC
    GATTGCATCA AGCTGGGCTG CTACAACGGT GGCACCTGCG TAACCACATC CGAAGGATCG
    CGGTGTGTTT GTCGTTTCGA CCGCCAAGGA CCTCTTTGCG AGCTGCCCAT TATCATCCGG
    AATGCCGCCT TCTCCGGCGA CTCCTATGTG TCGCACCGCA TCTACAAGGA CATCGGGGGC
    CACGAGTCCT TGGACGCCGT CCTGCCGATG CACATCCAGC TGAAGGTGCG AACCCGGGCC
    ACCAACGGGC TGATCATGCT GGCGGCGGCG CAGGGCACCA AGGGTGGCCA CTACATGGCC
    CTTTTCCTGC AGAAGGGACT CATGCAGTTC CAGTTCTCCT GCGGACTGCA GACGATGCTG
    CTGAGTGAAC TGGAAACGCC GGTCAACACC GGCCACGAAA TCACCATTCG AGCTGAATTG
    GACTTCAGCC GGAATTACAC ACACTGCAAC GCCTCGTTGC TGGTGAACGA CACGTTGGCC
    ATGTCCGGGG ATCAGCCCAC CTGGCTGAAG CTACTACCGC CGCGCCTTCA CACCCCGGAG
    GCCATCCTCA ACACCTGGCT GCATCTGGGC GGAGCGCCCC AGGCGCCGAT TGGCCTGATT
    ATCGAACTGC CGCCGGCCCA AAGTGGAAGC GGCTTCACCG GCTGCCTCCA TACGCTGCGC
    ATCAATGGAC AGGCTCGCGA GATTTTCGGC GATGCCCTCG ATGGCTTTGG CATTACGGAA
    TGCGGCTCAT TGGCGTGTCT ATCGAGTCCT TGCCGCAATG GCGCCGCCTG CATAAAGATC
    GAGACCAACG ATCTGGACGA GAATGGCGAG AAGGCGGAGA AGTGGAAGTG CAAGTGCCCC
    ACCGGCTACA TGGGCCCCAC CTGCGAGATA TCCGTGTGCG AGGACAACCC GTGCCAGTAC
    GGTGGAACCT GCGTACAATT TCCGGGCAGC GGTTACCTCT GCCTGTGTCC GCTGGGCAAG
    CACGGCCACT ATTGCGAGCA CAATCTTGAA GTGGCCCTGC CATCCTTCTC CGGCAGCGTC
    AATGGACTCT CCTCGTTTGT GGCCTACACG GTGCCGATTC CCCTCGAGTA TTCGCTCGAG
    CTGAGCTTCA AAATCCTGCC GCAGACCATG TCTCAGATTT CGCTGCTGGC CTTCTTCGGG
    CAGTCGGGTT ATCACGACGA GAAGAGCGAT CACCTGGCGG TGAGCTTCAT CCAGGGCTAC
    ATAATGCTCA CCTGGAATCT GGGAGCCGGG CCCCGTCGCA TATTCACACA GAAACCCATT
    GATTTCCGGC TGGATGCTCC CCGGGTTCCG TACGAGATTA AGGTGGGCCG GATTGGAAGA
    CAGGCCTGGC TCTCGGTGGA TGGAAAGTTC AATATTACGG GTCGATCGCC CGGAAGTGGC
    AGTCGGATGG ATGTGTTGCC TATTCTGTAT CTCGGCGGTC ACGAGATAGC CAATTTCAAT
    ACGCTGCCAC ACGATTTGCC ACTGCATTCG GGATTCCAGG GATGCATATA CGATGTACAG
    CTGAAGGCGG GACAGGTCAC GGTGCCCCTC CAGGAGACGC GCGGGGTCAG AGGTCGGGGC
    GTGGGTCAAT GTGGAACCAG GGAATGCCAT CGCCATGCCT GCCAGCACGA TGGCGCCTGC
    CTGCAGCATG GAGCAACCTT TACTTGCATC TGCCAGGAGG GCTGGTATGG GCCACTGTGT
    GCCCAGCCCA CGAATCCCTG TGACTCCTTC AACAACAAAT GCTACGAGGA CGCCACCTGT
    GTGCCGCTGG TCAATGGCTA CGAATGCGAT TGTCCTGTAG GACGCACAGG CAAAAACTGC
    GAGGAGGTAA TTCGATCCCT GAGCGACGTA TCCCTGACTG GCAGACGATC CTATCTGGCG
    GTTCGCTGGC CATATCTATA CGATGGCGGG GATAAACTGG GCGCTAAGCG CTCCCAAATG
    GTCAGCTACC GGAACTTCAC CAAGAAACTA ATGCCCCCGA AGCCCATTAC CACGCCCAGC
    AGCCACTTTG TGATGAAACT GCTGAATGAG GTGGAGAAGC AGCGCAGCTT CTCGCCAGTT
    CCCCTGATGG GATCGAAGTC CTTCGAGGAG CACCATCGAG TGCAGTTCTT CTTCATCGAG
    TTCCAACTGC GCCCGCTCTC GGAAAGAGGC CTCCTTCTGT ACTTCGGTAC CCTGAACAAC
    AATCAGGACA AGAAGATAGG ATTCGTGTCG CTATCGCTGC AGGGCGGAGT GGTGGAGTTC
    CGGATTTCCG GGCCCAGCAA TCATGTCACG GTGGTGCGGA GCGTTCGCAT GTTGGCCATC
    GGCGAGTGGC ACAAGATCAA GATGGCGCAG CGCGGAAGAT GGCTGACCCT GTGGGTGGAG
    GGTAGCGCCT CGTCGGCTTT GGCTCCTTCG GCTGAGGTGC TCGTCGAGCC CGACTCACTG
    CTCTACATTG GCGGGCTGAA GGATGTGTCC AAGCTGCCGC ACAACGCCAT CTCCGGTTTC
    CCGATTCCGT TCAGGGGATG TGTCCGGGGT CTGGTGGTCA GTGGAACTCG CATTGTGCTC
    AACGAAACCA ACATTGTGGA ATCGCGCAAC ATTCGAGACT GTGATGGCAC CGCTTGTGGC
    GGAGACTCCT GCGAGTCCGG AGGACATTGC TGGCTGGACG AGAAGCTGCA GCCGCATTGC
    ATCTGTCCGG AGTACGCGAA AGGAGATCGA TGCGAGTACT CGGAGACCTG CAAGCTCATT
    CCGTGCAAAA ACAATGGAAG ATGCCTGAGA AGTGGACGCT GCTCGTGTCC AAATGGATGG
    GGTGGCTTCT ACTGCGAGAT TGCCATGAGC AAGCCGACGA CGCCGAGCTT CCGTGGCAAC
    AGTTACCTGA TCTTGCCGCC GCCGCGAATT CCGATGAAAG ACAAGCGGCG GGGACCCTCC
    CTGTACGTCC GCCCCCGCGA AGCCATCCAA GTCTCGCTGA ACTTCTCCAC CATCGAACCG
    GACGGACTGC TCCTGTGGAG CGAGCACGAG AGGAGCAAGT TCCTCGGCCT GGGCCTGGAG
    GCGGGCCACC TCAAGCTGGC CAGCAACCTC CTGGGCAGCA CCAACGACAC GGTCCGTGCT
    CCGGCCAGCG GATTCATAGC CGACGGAGCC TGGCACTGGA CCAGCGTCCT GCTGGACCGG
    TCCAGGCTGG AGCTGCAGCT GGACGGCGAG GTGATCTTCA CGGAGCGATT GCCCGAGGGC
    GGACGGTCAC TGGGCTCGAC CACGCCGAGA AGCACTCTGG CGGGCAGGCG CAAGAATTCG
    AGCAAGGAGC CAACAATTAG CTACGAGGAT GTCTTCTACT TGGGCGGCTT TCCCAACTCG
    GACTCGGTGA GCAGGCGCAC AAAGGGTCGC TTCTTCGATC CCTTCAAGGG CTGCCTGCAG
    GATATCCAGT TCGGAGCCGA GCCCACAGCG ATTATCAGCG ACTTCTCGAC GTACCAGGGC
    GAGAACATTG GATCCTGTGA TCTGCACGGC GATGAGCCGC TTACTGTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa22383
    Clone ID Related Accession (Same CDS sequence) NM_001272995.1 , NM_001032399.3
    Accession Version NM_001032399.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6531bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product eyes shut, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001032399.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGAGCAACG TGCATCAGTT TGATACGCAG ACGATGGCGG AATCGCCTCA AATTAGACGC 
    GACATGGGTC GCCTATGTGC CACGTGGCCT TCGAAGGACT CTGAGGACGG GGCCGGCACA
    GCTTTGAGGG CAGCCACACC TCTGACAGCC AACGGAGCCA CCACCACCGG GCTGAGTGTT
    ACCCTTGCCC CGAAGGACAT GCAACGAAAC CACCTGCTAA AGATGCCAAC AGCAACAATA
    GAGAAACCAA CGATAACAGC CACCATCGCC AGCAGCAGCA GCACCAGCAC TAGCACCACT
    AGAAAATCAG TTACAGCCAC ACGGAGCCTG AAGTTGAACC CGAACATTCT CCTTCCGACA
    CTAAGGATAT TAGCAAGGGG TCTGCTGCTA CCCGCTCTGA TCCTGGCCAT CCTCGTGGGC
    AGCAGCCAGG CGGGATTTGC CTGCCTCAGC AATCCCTGTG TGTTCGGCGT GTGCATCGAT
    GGCCTAAACA GCTCGTACTC GTGTTACTGC ATTGATGGCT ACACAGGGAT CCAGTGCCAA
    ACGAATTGGG ACGAGTGCTG GTCGAGTCCT TGCCAGAACG GCGGGACATG TGTGGACGGT
    GTGGCCTACT ACAATTGTAC GTGTCCGGAA GGATTTTCTG GATCCAACTG CGAGGAGAAC
    GTCGACGAAT GCATGTCGAA TCCCTGCCAG AACGGCGGAC TCTGCCGGGA CAGAACCAAT
    GGCTACATAT GCACCTGCCA ACCAGGTTAC CTGGGATCTC ACTGCGAACT GGATGTCGCT
    GTCTGCGAGA CAGGAACTGG TGCTCGTTGC CAGCACGGTG GTGAGTGCAT CGAGGGTCCT
    GGACTGGAGT TCACCTGCGA CTGTCCGGCG GGCTGGCATG GTCGGATTTG CCAGGAGGAG
    ATTAACGAGT GCGCCTCGTC GCCCTGCCAA AATGGAGGCG TCTGCGTGGA CAAGCTGGCG
    GCCTATGCCT GCGCCTGTCC CATGGGATAT ACGGGCATCA ACTGCGAGGA GGAGATCCTC
    ATCTGTGCGG ACAATCCGTG TCAGAATAAT GCCTTGTGCT TGATGGAGGA GGGTGTACCC
    ACGTGCTATT GTGTACCCGA CTATCATGGC GAGAAGTGTG AGTTCCAGTA CGACGAATGC
    CAATTGGGAC CGCGGTGCAT GAATGGTGGT GTTTGCATCG ACGGTGTCGA TACCTTCTCC
    TGCTCATGTC CTCCCCTATT GACCGGTATG CTCTGCGAGT GCCTGATGGT GGGTGAGGAG
    TCCCTGGACT GCAACTACAC AGCACCGGCC ACGCAATCTC CGCCACGGAG AACTACCACC
    ACATCCACAA TGGCTCCACC CACAGTGAGG CCCGTCACTC CGCCGGAGAC AACGGTATCG
    CCATCCAGAG CTTCAGAAGA GGTGGAGATT ATAGTAGTTA CCACATCTGC TCCTGCGGAA
    GTGGTCACCT CTGTGCTATC CCCATCATCC TCTTCTTCAT CGTCGGAGGA AGGCGTTTCC
    GTCGAGATTA AAACACCGAC TGTGGCGCCC CCAGAAAGCG GTAGCCATAG CATATCCGTG
    GAGCAAACAA CGGCCGTTCC AGCACAACCC GAACCCGAAT CCGAACAAGA ACCAGAATCT
    AAGCCACATC CCGAATCGGA ATCGGCGTCG GAATCGGAAA CGGAAACGGA GGAGGAAATA
    ATTCCGGGAA CTACAGCGCG ACCACCAACA AGCAGATCCT CCAGTTCTTC GGAGGAATCT
    CCCAGCATCT TCACCACTTT GCCTCCGTTG CCGGGAAAGC CTCAAACGTC TGCGTCGTCG
    GAAAGTTCCG GAGAAGTGGT TACCTCCGAG GAATACACCA CTGTGCCGCA CTTCGAAGTG
    AGTGGCAGCA AAAGTGAAAG TGGTAGTGAA GAGGTCACCA CTGTGCGACC CACTGCAGCA
    CCGAGTATCA CCATTTCAGT GGACATCACC TCATCCGGAA GTAGCAGTAG TAGTTCGGAA
    TCAGTGGAGG TATTCACCAC ACCAGCTCCC GTTTTCGTGC AGAGAGTGAC TACGATCGAA
    ACTAGCATCT CCATAGACTA TGTCACACCC ACTCCTCTGC CGGAAACCAC CACCCCACGG
    GTGGTCCCGG TGCCCAGGCC TACCTTTGCC CCAGAGCCAC CACTGGATGT GGTGGAAACG
    ACTGCCTCAA CTCACCACCT TTGGACAGAG GTGCCCACCA CGGCGGCTCC CTTCTTCACG
    GAGTATCCGG CGGAAGTGCT GATCACTACA CATCGAACGA GTGCTGGCAG ATTCACCACA
    GTTCAACCAC CTGCAGGTGT CACCACCACC TCACCCACTG AGGACTCATC CGTTGAGTTA
    CCCACTCCGC ACACGCCCCA AATTGTGGTC ACCATTTTGG ATTCAAATGA AGTTATACCT
    TCGCTGATCA CAACCACTGG ATCCCCGACA ACACACCATC ACCACCATCA TCATCCTCAC
    CATGAAGCCG AAGGAACCAC TCTTCAGCCT CTCGAAGAAG ACGAGCACCA CCATCATCAT
    CATCATGACG AGTTCACCAC TCCACAGCCA GTGGAAATAA CTACGGGACA TCCTTTGCAA
    ACAGAGGACC TTATTGGAGT GCAGGAACCT GCCGTGGTGA CCACAGAGTC GCCCTTTGCT
    CCAGCTGAGA CAACTGTGGT TCCTGTGGTG GTGCCCGCTA CGATTGCACC ACTTGGAACT
    GCAGCACCAC CAGCCACACC AGCTCCTGTT CCTCCAGCGA CTACCACTCC TCCTCCTTCG
    CCTCCTTCCT TGGCTACAGA GACACCTACA CTGCCACCCA CTTTACCTCC TGTTACCCTG
    CCACCGGTAA CCCAGCCGCC GCCCACGATA CCACCCACTC CTCCATCCAC ACAATCCGCA
    CAAACTTTAC CGCCACCGAC GTCGGCCATA AATGTTTACA CCACTCCCGA TGGACCACCC
    ACGGCTTCCC AAACGAAGCC ATCGGTCACG GAGAGCAGCG AGGAAGTGGA GGGCACAAAT
    ACGGTCTCCA CCGGCGGAAG GGGATCCGGC GGAGTGCCCG AGGAGAAGGC AGGCGATGTC
    GATTGCATCA AGCTGGGCTG CTACAACGGT GGCACCTGCG TAACCACATC CGAAGGATCG
    CGGTGTGTTT GTCGTTTCGA CCGCCAAGGA CCTCTTTGCG AGCTGCCCAT TATCATCCGG
    AATGCCGCCT TCTCCGGCGA CTCCTATGTG TCGCACCGCA TCTACAAGGA CATCGGGGGC
    CACGAGTCCT TGGACGCCGT CCTGCCGATG CACATCCAGC TGAAGGTGCG AACCCGGGCC
    ACCAACGGGC TGATCATGCT GGCGGCGGCG CAGGGCACCA AGGGTGGCCA CTACATGGCC
    CTTTTCCTGC AGAAGGGACT CATGCAGTTC CAGTTCTCCT GCGGACTGCA GACGATGCTG
    CTGAGTGAAC TGGAAACGCC GGTCAACACC GGCCACGAAA TCACCATTCG AGCTGAATTG
    GACTTCAGCC GGAATTACAC ACACTGCAAC GCCTCGTTGC TGGTGAACGA CACGTTGGCC
    ATGTCCGGGG ATCAGCCCAC CTGGCTGAAG CTACTACCGC CGCGCCTTCA CACCCCGGAG
    GCCATCCTCA ACACCTGGCT GCATCTGGGC GGAGCGCCCC AGGCGCCGAT TGGCCTGATT
    ATCGAACTGC CGCCGGCCCA AAGTGGAAGC GGCTTCACCG GCTGCCTCCA TACGCTGCGC
    ATCAATGGAC AGGCTCGCGA GATTTTCGGC GATGCCCTCG ATGGCTTTGG CATTACGGAA
    TGCGGCTCAT TGGCGTGTCT ATCGAGTCCT TGCCGCAATG GCGCCGCCTG CATAAAGATC
    GAGACCAACG ATCTGGACGA GAATGGCGAG AAGGCGGAGA AGTGGAAGTG CAAGTGCCCC
    ACCGGCTACA TGGGCCCCAC CTGCGAGATA TCCGTGTGCG AGGACAACCC GTGCCAGTAC
    GGTGGAACCT GCGTACAATT TCCGGGCAGC GGTTACCTCT GCCTGTGTCC GCTGGGCAAG
    CACGGCCACT ATTGCGAGCA CAATCTTGAA GTGGCCCTGC CATCCTTCTC CGGCAGCGTC
    AATGGACTCT CCTCGTTTGT GGCCTACACG GTGCCGATTC CCCTCGAGTA TTCGCTCGAG
    CTGAGCTTCA AAATCCTGCC GCAGACCATG TCTCAGATTT CGCTGCTGGC CTTCTTCGGG
    CAGTCGGGTT ATCACGACGA GAAGAGCGAT CACCTGGCGG TGAGCTTCAT CCAGGGCTAC
    ATAATGCTCA CCTGGAATCT GGGAGCCGGG CCCCGTCGCA TATTCACACA GAAACCCATT
    GATTTCCGGC TGGATGCTCC CCGGGTTCCG TACGAGATTA AGGTGGGCCG GATTGGAAGA
    CAGGCCTGGC TCTCGGTGGA TGGAAAGTTC AATATTACGG GTCGATCGCC CGGAAGTGGC
    AGTCGGATGG ATGTGTTGCC TATTCTGTAT CTCGGCGGTC ACGAGATAGC CAATTTCAAT
    ACGCTGCCAC ACGATTTGCC ACTGCATTCG GGATTCCAGG GATGCATATA CGATGTACAG
    CTGAAGGCGG GACAGGTCAC GGTGCCCCTC CAGGAGACGC GCGGGGTCAG AGGTCGGGGC
    GTGGGTCAAT GTGGAACCAG GGAATGCCAT CGCCATGCCT GCCAGCACGA TGGCGCCTGC
    CTGCAGCATG GAGCAACCTT TACTTGCATC TGCCAGGAGG GCTGGTATGG GCCACTGTGT
    GCCCAGCCCA CGAATCCCTG TGACTCCTTC AACAACAAAT GCTACGAGGA CGCCACCTGT
    GTGCCGCTGG TCAATGGCTA CGAATGCGAT TGTCCTGTAG GACGCACAGG CAAAAACTGC
    GAGGAGGTAA TTCGATCCCT GAGCGACGTA TCCCTGACTG GCAGACGATC CTATCTGGCG
    GTTCGCTGGC CATATCTATA CGATGGCGGG GATAAACTGG GCGCTAAGCG CTCCCAAATG
    GTCAGCTACC GGAACTTCAC CAAGAAACTA ATGCCCCCGA AGCCCATTAC CACGCCCAGC
    AGCCACTTTG TGATGAAACT GCTGAATGAG GTGGAGAAGC AGCGCAGCTT CTCGCCAGTT
    CCCCTGATGG GATCGAAGTC CTTCGAGGAG CACCATCGAG TGCAGTTCTT CTTCATCGAG
    TTCCAACTGC GCCCGCTCTC GGAAAGAGGC CTCCTTCTGT ACTTCGGTAC CCTGAACAAC
    AATCAGGACA AGAAGATAGG ATTCGTGTCG CTATCGCTGC AGGGCGGAGT GGTGGAGTTC
    CGGATTTCCG GGCCCAGCAA TCATGTCACG GTGGTGCGGA GCGTTCGCAT GTTGGCCATC
    GGCGAGTGGC ACAAGATCAA GATGGCGCAG CGCGGAAGAT GGCTGACCCT GTGGGTGGAG
    GGTAGCGCCT CGTCGGCTTT GGCTCCTTCG GCTGAGGTGC TCGTCGAGCC CGACTCACTG
    CTCTACATTG GCGGGCTGAA GGATGTGTCC AAGCTGCCGC ACAACGCCAT CTCCGGTTTC
    CCGATTCCGT TCAGGGGATG TGTCCGGGGT CTGGTGGTCA GTGGAACTCG CATTGTGCTC
    AACGAAACCA ACATTGTGGA ATCGCGCAAC ATTCGAGACT GTGATGGCAC CGCTTGTGGC
    GGAGACTCCT GCGAGTCCGG AGGACATTGC TGGCTGGACG AGAAGCTGCA GCCGCATTGC
    ATCTGTCCGG AGTACGCGAA AGGAGATCGA TGCGAGTACT CGGAGACCTG CAAGCTCATT
    CCGTGCAAAA ACAATGGAAG ATGCCTGAGA AGTGGACGCT GCTCGTGTCC AAATGGATGG
    GGTGGCTTCT ACTGCGAGAT TGCCATGAGC AAGCCGACGA CGCCGAGCTT CCGTGGCAAC
    AGTTACCTGA TCTTGCCGCC GCCGCGAATT CCGATGAAAG ACAAGCGGCG GGGACCCTCC
    CTGTACGTCC GCCCCCGCGA AGCCATCCAA GTCTCGCTGA ACTTCTCCAC CATCGAACCG
    GACGGACTGC TCCTGTGGAG CGAGCACGAG AGGAGCAAGT TCCTCGGCCT GGGCCTGGAG
    GCGGGCCACC TCAAGCTGGC CAGCAACCTC CTGGGCAGCA CCAACGACAC GGTCCGTGCT
    CCGGCCAGCG GATTCATAGC CGACGGAGCC TGGCACTGGA CCAGCGTCCT GCTGGACCGG
    TCCAGGCTGG AGCTGCAGCT GGACGGCGAG GTGATCTTCA CGGAGCGATT GCCCGAGGGC
    GGACGGTCAC TGGGCTCGAC CACGCCGAGA AGCACTCTGG CGGGCAGGCG CAAGAATTCG
    AGCAAGGAGC CAACAATTAG CTACGAGGAT GTCTTCTACT TGGGCGGCTT TCCCAACTCG
    GACTCGGTGA GCAGGCGCAC AAAGGGTCGC TTCTTCGATC CCTTCAAGGG CTGCCTGCAG
    GATATCCAGT TCGGAGCCGA GCCCACAGCG ATTATCAGCG ACTTCTCGAC GTACCAGGGC
    GAGAACATTG GATCCTGTGA TCTGCACGGC GATGAGCCGC TTACTGTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001027571.3
    CDS884..7414
    Translation

    Target ORF information:

    RefSeq Version NM_001032399.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster eyes shut (eys), transcript variant F, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001032399.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGAGCAACG TGCATCAGTT TGATACGCAG ACGATGGCGG AATCGCCTCA AATTAGACGC 
    GACATGGGTC GCCTATGTGC CACGTGGCCT TCGAAGGACT CTGAGGACGG GGCCGGCACA
    GCTTTGAGGG CAGCCACACC TCTGACAGCC AACGGAGCCA CCACCACCGG GCTGAGTGTT
    ACCCTTGCCC CGAAGGACAT GCAACGAAAC CACCTGCTAA AGATGCCAAC AGCAACAATA
    GAGAAACCAA CGATAACAGC CACCATCGCC AGCAGCAGCA GCACCAGCAC TAGCACCACT
    AGAAAATCAG TTACAGCCAC ACGGAGCCTG AAGTTGAACC CGAACATTCT CCTTCCGACA
    CTAAGGATAT TAGCAAGGGG TCTGCTGCTA CCCGCTCTGA TCCTGGCCAT CCTCGTGGGC
    AGCAGCCAGG CGGGATTTGC CTGCCTCAGC AATCCCTGTG TGTTCGGCGT GTGCATCGAT
    GGCCTAAACA GCTCGTACTC GTGTTACTGC ATTGATGGCT ACACAGGGAT CCAGTGCCAA
    ACGAATTGGG ACGAGTGCTG GTCGAGTCCT TGCCAGAACG GCGGGACATG TGTGGACGGT
    GTGGCCTACT ACAATTGTAC GTGTCCGGAA GGATTTTCTG GATCCAACTG CGAGGAGAAC
    GTCGACGAAT GCATGTCGAA TCCCTGCCAG AACGGCGGAC TCTGCCGGGA CAGAACCAAT
    GGCTACATAT GCACCTGCCA ACCAGGTTAC CTGGGATCTC ACTGCGAACT GGATGTCGCT
    GTCTGCGAGA CAGGAACTGG TGCTCGTTGC CAGCACGGTG GTGAGTGCAT CGAGGGTCCT
    GGACTGGAGT TCACCTGCGA CTGTCCGGCG GGCTGGCATG GTCGGATTTG CCAGGAGGAG
    ATTAACGAGT GCGCCTCGTC GCCCTGCCAA AATGGAGGCG TCTGCGTGGA CAAGCTGGCG
    GCCTATGCCT GCGCCTGTCC CATGGGATAT ACGGGCATCA ACTGCGAGGA GGAGATCCTC
    ATCTGTGCGG ACAATCCGTG TCAGAATAAT GCCTTGTGCT TGATGGAGGA GGGTGTACCC
    ACGTGCTATT GTGTACCCGA CTATCATGGC GAGAAGTGTG AGTTCCAGTA CGACGAATGC
    CAATTGGGAC CGCGGTGCAT GAATGGTGGT GTTTGCATCG ACGGTGTCGA TACCTTCTCC
    TGCTCATGTC CTCCCCTATT GACCGGTATG CTCTGCGAGT GCCTGATGGT GGGTGAGGAG
    TCCCTGGACT GCAACTACAC AGCACCGGCC ACGCAATCTC CGCCACGGAG AACTACCACC
    ACATCCACAA TGGCTCCACC CACAGTGAGG CCCGTCACTC CGCCGGAGAC AACGGTATCG
    CCATCCAGAG CTTCAGAAGA GGTGGAGATT ATAGTAGTTA CCACATCTGC TCCTGCGGAA
    GTGGTCACCT CTGTGCTATC CCCATCATCC TCTTCTTCAT CGTCGGAGGA AGGCGTTTCC
    GTCGAGATTA AAACACCGAC TGTGGCGCCC CCAGAAAGCG GTAGCCATAG CATATCCGTG
    GAGCAAACAA CGGCCGTTCC AGCACAACCC GAACCCGAAT CCGAACAAGA ACCAGAATCT
    AAGCCACATC CCGAATCGGA ATCGGCGTCG GAATCGGAAA CGGAAACGGA GGAGGAAATA
    ATTCCGGGAA CTACAGCGCG ACCACCAACA AGCAGATCCT CCAGTTCTTC GGAGGAATCT
    CCCAGCATCT TCACCACTTT GCCTCCGTTG CCGGGAAAGC CTCAAACGTC TGCGTCGTCG
    GAAAGTTCCG GAGAAGTGGT TACCTCCGAG GAATACACCA CTGTGCCGCA CTTCGAAGTG
    AGTGGCAGCA AAAGTGAAAG TGGTAGTGAA GAGGTCACCA CTGTGCGACC CACTGCAGCA
    CCGAGTATCA CCATTTCAGT GGACATCACC TCATCCGGAA GTAGCAGTAG TAGTTCGGAA
    TCAGTGGAGG TATTCACCAC ACCAGCTCCC GTTTTCGTGC AGAGAGTGAC TACGATCGAA
    ACTAGCATCT CCATAGACTA TGTCACACCC ACTCCTCTGC CGGAAACCAC CACCCCACGG
    GTGGTCCCGG TGCCCAGGCC TACCTTTGCC CCAGAGCCAC CACTGGATGT GGTGGAAACG
    ACTGCCTCAA CTCACCACCT TTGGACAGAG GTGCCCACCA CGGCGGCTCC CTTCTTCACG
    GAGTATCCGG CGGAAGTGCT GATCACTACA CATCGAACGA GTGCTGGCAG ATTCACCACA
    GTTCAACCAC CTGCAGGTGT CACCACCACC TCACCCACTG AGGACTCATC CGTTGAGTTA
    CCCACTCCGC ACACGCCCCA AATTGTGGTC ACCATTTTGG ATTCAAATGA AGTTATACCT
    TCGCTGATCA CAACCACTGG ATCCCCGACA ACACACCATC ACCACCATCA TCATCCTCAC
    CATGAAGCCG AAGGAACCAC TCTTCAGCCT CTCGAAGAAG ACGAGCACCA CCATCATCAT
    CATCATGACG AGTTCACCAC TCCACAGCCA GTGGAAATAA CTACGGGACA TCCTTTGCAA
    ACAGAGGACC TTATTGGAGT GCAGGAACCT GCCGTGGTGA CCACAGAGTC GCCCTTTGCT
    CCAGCTGAGA CAACTGTGGT TCCTGTGGTG GTGCCCGCTA CGATTGCACC ACTTGGAACT
    GCAGCACCAC CAGCCACACC AGCTCCTGTT CCTCCAGCGA CTACCACTCC TCCTCCTTCG
    CCTCCTTCCT TGGCTACAGA GACACCTACA CTGCCACCCA CTTTACCTCC TGTTACCCTG
    CCACCGGTAA CCCAGCCGCC GCCCACGATA CCACCCACTC CTCCATCCAC ACAATCCGCA
    CAAACTTTAC CGCCACCGAC GTCGGCCATA AATGTTTACA CCACTCCCGA TGGACCACCC
    ACGGCTTCCC AAACGAAGCC ATCGGTCACG GAGAGCAGCG AGGAAGTGGA GGGCACAAAT
    ACGGTCTCCA CCGGCGGAAG GGGATCCGGC GGAGTGCCCG AGGAGAAGGC AGGCGATGTC
    GATTGCATCA AGCTGGGCTG CTACAACGGT GGCACCTGCG TAACCACATC CGAAGGATCG
    CGGTGTGTTT GTCGTTTCGA CCGCCAAGGA CCTCTTTGCG AGCTGCCCAT TATCATCCGG
    AATGCCGCCT TCTCCGGCGA CTCCTATGTG TCGCACCGCA TCTACAAGGA CATCGGGGGC
    CACGAGTCCT TGGACGCCGT CCTGCCGATG CACATCCAGC TGAAGGTGCG AACCCGGGCC
    ACCAACGGGC TGATCATGCT GGCGGCGGCG CAGGGCACCA AGGGTGGCCA CTACATGGCC
    CTTTTCCTGC AGAAGGGACT CATGCAGTTC CAGTTCTCCT GCGGACTGCA GACGATGCTG
    CTGAGTGAAC TGGAAACGCC GGTCAACACC GGCCACGAAA TCACCATTCG AGCTGAATTG
    GACTTCAGCC GGAATTACAC ACACTGCAAC GCCTCGTTGC TGGTGAACGA CACGTTGGCC
    ATGTCCGGGG ATCAGCCCAC CTGGCTGAAG CTACTACCGC CGCGCCTTCA CACCCCGGAG
    GCCATCCTCA ACACCTGGCT GCATCTGGGC GGAGCGCCCC AGGCGCCGAT TGGCCTGATT
    ATCGAACTGC CGCCGGCCCA AAGTGGAAGC GGCTTCACCG GCTGCCTCCA TACGCTGCGC
    ATCAATGGAC AGGCTCGCGA GATTTTCGGC GATGCCCTCG ATGGCTTTGG CATTACGGAA
    TGCGGCTCAT TGGCGTGTCT ATCGAGTCCT TGCCGCAATG GCGCCGCCTG CATAAAGATC
    GAGACCAACG ATCTGGACGA GAATGGCGAG AAGGCGGAGA AGTGGAAGTG CAAGTGCCCC
    ACCGGCTACA TGGGCCCCAC CTGCGAGATA TCCGTGTGCG AGGACAACCC GTGCCAGTAC
    GGTGGAACCT GCGTACAATT TCCGGGCAGC GGTTACCTCT GCCTGTGTCC GCTGGGCAAG
    CACGGCCACT ATTGCGAGCA CAATCTTGAA GTGGCCCTGC CATCCTTCTC CGGCAGCGTC
    AATGGACTCT CCTCGTTTGT GGCCTACACG GTGCCGATTC CCCTCGAGTA TTCGCTCGAG
    CTGAGCTTCA AAATCCTGCC GCAGACCATG TCTCAGATTT CGCTGCTGGC CTTCTTCGGG
    CAGTCGGGTT ATCACGACGA GAAGAGCGAT CACCTGGCGG TGAGCTTCAT CCAGGGCTAC
    ATAATGCTCA CCTGGAATCT GGGAGCCGGG CCCCGTCGCA TATTCACACA GAAACCCATT
    GATTTCCGGC TGGATGCTCC CCGGGTTCCG TACGAGATTA AGGTGGGCCG GATTGGAAGA
    CAGGCCTGGC TCTCGGTGGA TGGAAAGTTC AATATTACGG GTCGATCGCC CGGAAGTGGC
    AGTCGGATGG ATGTGTTGCC TATTCTGTAT CTCGGCGGTC ACGAGATAGC CAATTTCAAT
    ACGCTGCCAC ACGATTTGCC ACTGCATTCG GGATTCCAGG GATGCATATA CGATGTACAG
    CTGAAGGCGG GACAGGTCAC GGTGCCCCTC CAGGAGACGC GCGGGGTCAG AGGTCGGGGC
    GTGGGTCAAT GTGGAACCAG GGAATGCCAT CGCCATGCCT GCCAGCACGA TGGCGCCTGC
    CTGCAGCATG GAGCAACCTT TACTTGCATC TGCCAGGAGG GCTGGTATGG GCCACTGTGT
    GCCCAGCCCA CGAATCCCTG TGACTCCTTC AACAACAAAT GCTACGAGGA CGCCACCTGT
    GTGCCGCTGG TCAATGGCTA CGAATGCGAT TGTCCTGTAG GACGCACAGG CAAAAACTGC
    GAGGAGGTAA TTCGATCCCT GAGCGACGTA TCCCTGACTG GCAGACGATC CTATCTGGCG
    GTTCGCTGGC CATATCTATA CGATGGCGGG GATAAACTGG GCGCTAAGCG CTCCCAAATG
    GTCAGCTACC GGAACTTCAC CAAGAAACTA ATGCCCCCGA AGCCCATTAC CACGCCCAGC
    AGCCACTTTG TGATGAAACT GCTGAATGAG GTGGAGAAGC AGCGCAGCTT CTCGCCAGTT
    CCCCTGATGG GATCGAAGTC CTTCGAGGAG CACCATCGAG TGCAGTTCTT CTTCATCGAG
    TTCCAACTGC GCCCGCTCTC GGAAAGAGGC CTCCTTCTGT ACTTCGGTAC CCTGAACAAC
    AATCAGGACA AGAAGATAGG ATTCGTGTCG CTATCGCTGC AGGGCGGAGT GGTGGAGTTC
    CGGATTTCCG GGCCCAGCAA TCATGTCACG GTGGTGCGGA GCGTTCGCAT GTTGGCCATC
    GGCGAGTGGC ACAAGATCAA GATGGCGCAG CGCGGAAGAT GGCTGACCCT GTGGGTGGAG
    GGTAGCGCCT CGTCGGCTTT GGCTCCTTCG GCTGAGGTGC TCGTCGAGCC CGACTCACTG
    CTCTACATTG GCGGGCTGAA GGATGTGTCC AAGCTGCCGC ACAACGCCAT CTCCGGTTTC
    CCGATTCCGT TCAGGGGATG TGTCCGGGGT CTGGTGGTCA GTGGAACTCG CATTGTGCTC
    AACGAAACCA ACATTGTGGA ATCGCGCAAC ATTCGAGACT GTGATGGCAC CGCTTGTGGC
    GGAGACTCCT GCGAGTCCGG AGGACATTGC TGGCTGGACG AGAAGCTGCA GCCGCATTGC
    ATCTGTCCGG AGTACGCGAA AGGAGATCGA TGCGAGTACT CGGAGACCTG CAAGCTCATT
    CCGTGCAAAA ACAATGGAAG ATGCCTGAGA AGTGGACGCT GCTCGTGTCC AAATGGATGG
    GGTGGCTTCT ACTGCGAGAT TGCCATGAGC AAGCCGACGA CGCCGAGCTT CCGTGGCAAC
    AGTTACCTGA TCTTGCCGCC GCCGCGAATT CCGATGAAAG ACAAGCGGCG GGGACCCTCC
    CTGTACGTCC GCCCCCGCGA AGCCATCCAA GTCTCGCTGA ACTTCTCCAC CATCGAACCG
    GACGGACTGC TCCTGTGGAG CGAGCACGAG AGGAGCAAGT TCCTCGGCCT GGGCCTGGAG
    GCGGGCCACC TCAAGCTGGC CAGCAACCTC CTGGGCAGCA CCAACGACAC GGTCCGTGCT
    CCGGCCAGCG GATTCATAGC CGACGGAGCC TGGCACTGGA CCAGCGTCCT GCTGGACCGG
    TCCAGGCTGG AGCTGCAGCT GGACGGCGAG GTGATCTTCA CGGAGCGATT GCCCGAGGGC
    GGACGGTCAC TGGGCTCGAC CACGCCGAGA AGCACTCTGG CGGGCAGGCG CAAGAATTCG
    AGCAAGGAGC CAACAATTAG CTACGAGGAT GTCTTCTACT TGGGCGGCTT TCCCAACTCG
    GACTCGGTGA GCAGGCGCAC AAAGGGTCGC TTCTTCGATC CCTTCAAGGG CTGCCTGCAG
    GATATCCAGT TCGGAGCCGA GCCCACAGCG ATTATCAGCG ACTTCTCGAC GTACCAGGGC
    GAGAACATTG GATCCTGTGA TCTGCACGGC GATGAGCCGC TTACTGTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.