eys cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	eys
Entrez Gene ID	3771890
Full Name	eyes shut
Synonyms	21A6,CG15388,CG15389,CG33955,CG7245,CT35437,Dmel\CG33955,EYS,Eyes Shut,Eys,MAb21A6,SPAM,Spam,mAb 21A6,mAb21A6,spacemaker,spam
General protein information	Preferred Names eyes shut Names CG33955 gene product from transcript CG33955-RF CG33955-PE CG33955-PF agrin-like eyeshut eys-PE eys-PF fibropellin spacemaker spam
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 22E1-22E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001272995.1	NP_001259924.2	eyes shut, isoform E
NM_001032399.3	NP_001027571.3	eyes shut, isoform F

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	eys	NP_001259924.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007900	XP_317590.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

our data suggest that O-glucosylation of Eys by Rumi ensures rhabdomere separation by promoting proper Eys folding and stability in a critical time window during the mid-pupal stage.
Title: The protein O-glucosyltransferase Rumi modifies eyes shut to promote rhabdomere separation in Drosophila.

Spacemaker, a secreted protein expressed only in the eyes of insects with open rhabdom systems, acts together with Prominin and the cell adhesion molecule Chaoptin to choreograph the partitioning of rhabdomeres into an open system.
Title: Transforming the architecture of compound eyes.

eys is essential for epithelial lumen formation in the retina.
Title: The agrin/perlecan-related protein eyes shut is essential for epithelial lumen formation in the Drosophila retina.

The following eys gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eys cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa22383	NM_001272995.1 Latest version!	Drosophila melanogaster eyes shut (eys), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa22383	NM_001032399.3 Latest version!	Drosophila melanogaster eyes shut (eys), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

eys ( NM_001272995.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259924.2 Drosophila melanogaster eyes shut (eys), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa22383
Clone ID Related Accession (Same CDS sequence)	NM_001272995.1 , NM_001032399.3
Accession Version	NM_001272995.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eyes shut, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCAACG TGCATCAGTT TGATACGCAG ACGATGGCGG AATCGCCTCA AATTAGACGC 
GACATGGGTC GCCTATGTGC CACGTGGCCT TCGAAGGACT CTGAGGACGG GGCCGGCACA 
GCTTTGAGGG CAGCCACACC TCTGACAGCC AACGGAGCCA CCACCACCGG GCTGAGTGTT 
ACCCTTGCCC CGAAGGACAT GCAACGAAAC CACCTGCTAA AGATGCCAAC AGCAACAATA 
GAGAAACCAA CGATAACAGC CACCATCGCC AGCAGCAGCA GCACCAGCAC TAGCACCACT 
AGAAAATCAG TTACAGCCAC ACGGAGCCTG AAGTTGAACC CGAACATTCT CCTTCCGACA 
CTAAGGATAT TAGCAAGGGG TCTGCTGCTA CCCGCTCTGA TCCTGGCCAT CCTCGTGGGC 
AGCAGCCAGG CGGGATTTGC CTGCCTCAGC AATCCCTGTG TGTTCGGCGT GTGCATCGAT 
GGCCTAAACA GCTCGTACTC GTGTTACTGC ATTGATGGCT ACACAGGGAT CCAGTGCCAA 
ACGAATTGGG ACGAGTGCTG GTCGAGTCCT TGCCAGAACG GCGGGACATG TGTGGACGGT 
GTGGCCTACT ACAATTGTAC GTGTCCGGAA GGATTTTCTG GATCCAACTG CGAGGAGAAC 
GTCGACGAAT GCATGTCGAA TCCCTGCCAG AACGGCGGAC TCTGCCGGGA CAGAACCAAT 
GGCTACATAT GCACCTGCCA ACCAGGTTAC CTGGGATCTC ACTGCGAACT GGATGTCGCT 
GTCTGCGAGA CAGGAACTGG TGCTCGTTGC CAGCACGGTG GTGAGTGCAT CGAGGGTCCT 
GGACTGGAGT TCACCTGCGA CTGTCCGGCG GGCTGGCATG GTCGGATTTG CCAGGAGGAG 
ATTAACGAGT GCGCCTCGTC GCCCTGCCAA AATGGAGGCG TCTGCGTGGA CAAGCTGGCG 
GCCTATGCCT GCGCCTGTCC CATGGGATAT ACGGGCATCA ACTGCGAGGA GGAGATCCTC 
ATCTGTGCGG ACAATCCGTG TCAGAATAAT GCCTTGTGCT TGATGGAGGA GGGTGTACCC 
ACGTGCTATT GTGTACCCGA CTATCATGGC GAGAAGTGTG AGTTCCAGTA CGACGAATGC 
CAATTGGGAC CGCGGTGCAT GAATGGTGGT GTTTGCATCG ACGGTGTCGA TACCTTCTCC 
TGCTCATGTC CTCCCCTATT GACCGGTATG CTCTGCGAGT GCCTGATGGT GGGTGAGGAG 
TCCCTGGACT GCAACTACAC AGCACCGGCC ACGCAATCTC CGCCACGGAG AACTACCACC 
ACATCCACAA TGGCTCCACC CACAGTGAGG CCCGTCACTC CGCCGGAGAC AACGGTATCG 
CCATCCAGAG CTTCAGAAGA GGTGGAGATT ATAGTAGTTA CCACATCTGC TCCTGCGGAA 
GTGGTCACCT CTGTGCTATC CCCATCATCC TCTTCTTCAT CGTCGGAGGA AGGCGTTTCC 
GTCGAGATTA AAACACCGAC TGTGGCGCCC CCAGAAAGCG GTAGCCATAG CATATCCGTG 
GAGCAAACAA CGGCCGTTCC AGCACAACCC GAACCCGAAT CCGAACAAGA ACCAGAATCT 
AAGCCACATC CCGAATCGGA ATCGGCGTCG GAATCGGAAA CGGAAACGGA GGAGGAAATA 
ATTCCGGGAA CTACAGCGCG ACCACCAACA AGCAGATCCT CCAGTTCTTC GGAGGAATCT 
CCCAGCATCT TCACCACTTT GCCTCCGTTG CCGGGAAAGC CTCAAACGTC TGCGTCGTCG 
GAAAGTTCCG GAGAAGTGGT TACCTCCGAG GAATACACCA CTGTGCCGCA CTTCGAAGTG 
AGTGGCAGCA AAAGTGAAAG TGGTAGTGAA GAGGTCACCA CTGTGCGACC CACTGCAGCA 
CCGAGTATCA CCATTTCAGT GGACATCACC TCATCCGGAA GTAGCAGTAG TAGTTCGGAA 
TCAGTGGAGG TATTCACCAC ACCAGCTCCC GTTTTCGTGC AGAGAGTGAC TACGATCGAA 
ACTAGCATCT CCATAGACTA TGTCACACCC ACTCCTCTGC CGGAAACCAC CACCCCACGG 
GTGGTCCCGG TGCCCAGGCC TACCTTTGCC CCAGAGCCAC CACTGGATGT GGTGGAAACG 
ACTGCCTCAA CTCACCACCT TTGGACAGAG GTGCCCACCA CGGCGGCTCC CTTCTTCACG 
GAGTATCCGG CGGAAGTGCT GATCACTACA CATCGAACGA GTGCTGGCAG ATTCACCACA 
GTTCAACCAC CTGCAGGTGT CACCACCACC TCACCCACTG AGGACTCATC CGTTGAGTTA 
CCCACTCCGC ACACGCCCCA AATTGTGGTC ACCATTTTGG ATTCAAATGA AGTTATACCT 
TCGCTGATCA CAACCACTGG ATCCCCGACA ACACACCATC ACCACCATCA TCATCCTCAC 
CATGAAGCCG AAGGAACCAC TCTTCAGCCT CTCGAAGAAG ACGAGCACCA CCATCATCAT 
CATCATGACG AGTTCACCAC TCCACAGCCA GTGGAAATAA CTACGGGACA TCCTTTGCAA 
ACAGAGGACC TTATTGGAGT GCAGGAACCT GCCGTGGTGA CCACAGAGTC GCCCTTTGCT 
CCAGCTGAGA CAACTGTGGT TCCTGTGGTG GTGCCCGCTA CGATTGCACC ACTTGGAACT 
GCAGCACCAC CAGCCACACC AGCTCCTGTT CCTCCAGCGA CTACCACTCC TCCTCCTTCG 
CCTCCTTCCT TGGCTACAGA GACACCTACA CTGCCACCCA CTTTACCTCC TGTTACCCTG 
CCACCGGTAA CCCAGCCGCC GCCCACGATA CCACCCACTC CTCCATCCAC ACAATCCGCA 
CAAACTTTAC CGCCACCGAC GTCGGCCATA AATGTTTACA CCACTCCCGA TGGACCACCC 
ACGGCTTCCC AAACGAAGCC ATCGGTCACG GAGAGCAGCG AGGAAGTGGA GGGCACAAAT 
ACGGTCTCCA CCGGCGGAAG GGGATCCGGC GGAGTGCCCG AGGAGAAGGC AGGCGATGTC 
GATTGCATCA AGCTGGGCTG CTACAACGGT GGCACCTGCG TAACCACATC CGAAGGATCG 
CGGTGTGTTT GTCGTTTCGA CCGCCAAGGA CCTCTTTGCG AGCTGCCCAT TATCATCCGG 
AATGCCGCCT TCTCCGGCGA CTCCTATGTG TCGCACCGCA TCTACAAGGA CATCGGGGGC 
CACGAGTCCT TGGACGCCGT CCTGCCGATG CACATCCAGC TGAAGGTGCG AACCCGGGCC 
ACCAACGGGC TGATCATGCT GGCGGCGGCG CAGGGCACCA AGGGTGGCCA CTACATGGCC 
CTTTTCCTGC AGAAGGGACT CATGCAGTTC CAGTTCTCCT GCGGACTGCA GACGATGCTG 
CTGAGTGAAC TGGAAACGCC GGTCAACACC GGCCACGAAA TCACCATTCG AGCTGAATTG 
GACTTCAGCC GGAATTACAC ACACTGCAAC GCCTCGTTGC TGGTGAACGA CACGTTGGCC 
ATGTCCGGGG ATCAGCCCAC CTGGCTGAAG CTACTACCGC CGCGCCTTCA CACCCCGGAG 
GCCATCCTCA ACACCTGGCT GCATCTGGGC GGAGCGCCCC AGGCGCCGAT TGGCCTGATT 
ATCGAACTGC CGCCGGCCCA AAGTGGAAGC GGCTTCACCG GCTGCCTCCA TACGCTGCGC 
ATCAATGGAC AGGCTCGCGA GATTTTCGGC GATGCCCTCG ATGGCTTTGG CATTACGGAA 
TGCGGCTCAT TGGCGTGTCT ATCGAGTCCT TGCCGCAATG GCGCCGCCTG CATAAAGATC 
GAGACCAACG ATCTGGACGA GAATGGCGAG AAGGCGGAGA AGTGGAAGTG CAAGTGCCCC 
ACCGGCTACA TGGGCCCCAC CTGCGAGATA TCCGTGTGCG AGGACAACCC GTGCCAGTAC 
GGTGGAACCT GCGTACAATT TCCGGGCAGC GGTTACCTCT GCCTGTGTCC GCTGGGCAAG 
CACGGCCACT ATTGCGAGCA CAATCTTGAA GTGGCCCTGC CATCCTTCTC CGGCAGCGTC 
AATGGACTCT CCTCGTTTGT GGCCTACACG GTGCCGATTC CCCTCGAGTA TTCGCTCGAG 
CTGAGCTTCA AAATCCTGCC GCAGACCATG TCTCAGATTT CGCTGCTGGC CTTCTTCGGG 
CAGTCGGGTT ATCACGACGA GAAGAGCGAT CACCTGGCGG TGAGCTTCAT CCAGGGCTAC 
ATAATGCTCA CCTGGAATCT GGGAGCCGGG CCCCGTCGCA TATTCACACA GAAACCCATT 
GATTTCCGGC TGGATGCTCC CCGGGTTCCG TACGAGATTA AGGTGGGCCG GATTGGAAGA 
CAGGCCTGGC TCTCGGTGGA TGGAAAGTTC AATATTACGG GTCGATCGCC CGGAAGTGGC 
AGTCGGATGG ATGTGTTGCC TATTCTGTAT CTCGGCGGTC ACGAGATAGC CAATTTCAAT 
ACGCTGCCAC ACGATTTGCC ACTGCATTCG GGATTCCAGG GATGCATATA CGATGTACAG 
CTGAAGGCGG GACAGGTCAC GGTGCCCCTC CAGGAGACGC GCGGGGTCAG AGGTCGGGGC 
GTGGGTCAAT GTGGAACCAG GGAATGCCAT CGCCATGCCT GCCAGCACGA TGGCGCCTGC 
CTGCAGCATG GAGCAACCTT TACTTGCATC TGCCAGGAGG GCTGGTATGG GCCACTGTGT 
GCCCAGCCCA CGAATCCCTG TGACTCCTTC AACAACAAAT GCTACGAGGA CGCCACCTGT 
GTGCCGCTGG TCAATGGCTA CGAATGCGAT TGTCCTGTAG GACGCACAGG CAAAAACTGC 
GAGGAGGTAA TTCGATCCCT GAGCGACGTA TCCCTGACTG GCAGACGATC CTATCTGGCG 
GTTCGCTGGC CATATCTATA CGATGGCGGG GATAAACTGG GCGCTAAGCG CTCCCAAATG 
GTCAGCTACC GGAACTTCAC CAAGAAACTA ATGCCCCCGA AGCCCATTAC CACGCCCAGC 
AGCCACTTTG TGATGAAACT GCTGAATGAG GTGGAGAAGC AGCGCAGCTT CTCGCCAGTT 
CCCCTGATGG GATCGAAGTC CTTCGAGGAG CACCATCGAG TGCAGTTCTT CTTCATCGAG 
TTCCAACTGC GCCCGCTCTC GGAAAGAGGC CTCCTTCTGT ACTTCGGTAC CCTGAACAAC 
AATCAGGACA AGAAGATAGG ATTCGTGTCG CTATCGCTGC AGGGCGGAGT GGTGGAGTTC 
CGGATTTCCG GGCCCAGCAA TCATGTCACG GTGGTGCGGA GCGTTCGCAT GTTGGCCATC 
GGCGAGTGGC ACAAGATCAA GATGGCGCAG CGCGGAAGAT GGCTGACCCT GTGGGTGGAG 
GGTAGCGCCT CGTCGGCTTT GGCTCCTTCG GCTGAGGTGC TCGTCGAGCC CGACTCACTG 
CTCTACATTG GCGGGCTGAA GGATGTGTCC AAGCTGCCGC ACAACGCCAT CTCCGGTTTC 
CCGATTCCGT TCAGGGGATG TGTCCGGGGT CTGGTGGTCA GTGGAACTCG CATTGTGCTC 
AACGAAACCA ACATTGTGGA ATCGCGCAAC ATTCGAGACT GTGATGGCAC CGCTTGTGGC 
GGAGACTCCT GCGAGTCCGG AGGACATTGC TGGCTGGACG AGAAGCTGCA GCCGCATTGC 
ATCTGTCCGG AGTACGCGAA AGGAGATCGA TGCGAGTACT CGGAGACCTG CAAGCTCATT 
CCGTGCAAAA ACAATGGAAG ATGCCTGAGA AGTGGACGCT GCTCGTGTCC AAATGGATGG 
GGTGGCTTCT ACTGCGAGAT TGCCATGAGC AAGCCGACGA CGCCGAGCTT CCGTGGCAAC 
AGTTACCTGA TCTTGCCGCC GCCGCGAATT CCGATGAAAG ACAAGCGGCG GGGACCCTCC 
CTGTACGTCC GCCCCCGCGA AGCCATCCAA GTCTCGCTGA ACTTCTCCAC CATCGAACCG 
GACGGACTGC TCCTGTGGAG CGAGCACGAG AGGAGCAAGT TCCTCGGCCT GGGCCTGGAG 
GCGGGCCACC TCAAGCTGGC CAGCAACCTC CTGGGCAGCA CCAACGACAC GGTCCGTGCT 
CCGGCCAGCG GATTCATAGC CGACGGAGCC TGGCACTGGA CCAGCGTCCT GCTGGACCGG 
TCCAGGCTGG AGCTGCAGCT GGACGGCGAG GTGATCTTCA CGGAGCGATT GCCCGAGGGC 
GGACGGTCAC TGGGCTCGAC CACGCCGAGA AGCACTCTGG CGGGCAGGCG CAAGAATTCG 
AGCAAGGAGC CAACAATTAG CTACGAGGAT GTCTTCTACT TGGGCGGCTT TCCCAACTCG 
GACTCGGTGA GCAGGCGCAC AAAGGGTCGC TTCTTCGATC CCTTCAAGGG CTGCCTGCAG 
GATATCCAGT TCGGAGCCGA GCCCACAGCG ATTATCAGCG ACTTCTCGAC GTACCAGGGC 
GAGAACATTG GATCCTGTGA TCTGCACGGC GATGAGCCGC TTACTGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259924.2
CDS	884..7414
Translation	MSNVHQFDTQTMAESPQIRRDMGRLCATWPSKDSEDGAGTALRAATPLTANGATTTGLSVTLAPKDMQRNHLLKMPTATIEKPTITATIASSSSTSTSTTRKSVTATRSLKLNPNILLPTLRILARGLLLPALILAILVGSSQAGFACLSNPCVFGVCIDGLNSSYSCYCIDGYTGIQCQTNWDECWSSPCQNGGTCVDGVAYYNCTCPEGFSGSNCEENVDECMSNPCQNGGLCRDRTNGYICTCQPGYLGSHCELDVAVCETGTGARCQHGGECIEGPGLEFTCDCPAGWHGRICQEEINECASSPCQNGGVCVDKLAAYACACPMGYTGINCEEEILICADNPCQNNALCLMEEGVPTCYCVPDYHGEKCEFQYDECQLGPRCMNGGVCIDGVDTFSCSCPPLLTGMLCECLMVGEESLDCNYTAPATQSPPRRTTTTSTMAPPTVRPVTPPETTVSPSRASEEVEIIVVTTSAPAEVVTSVLSPSSSSSSSEEGVSVEIKTPTVAPPESGSHSISVEQTTAVPAQPEPESEQEPESKPHPESESASESETETEEEIIPGTTARPPTSRSSSSSEESPSIFTTLPPLPGKPQTSASSESSGEVVTSEEYTTVPHFEVSGSKSESGSEEVTTVRPTAAPSITISVDITSSGSSSSSSESVEVFTTPAPVFVQRVTTIETSISIDYVTPTPLPETTTPRVVPVPRPTFAPEPPLDVVETTASTHHLWTEVPTTAAPFFTEYPAEVLITTHRTSAGRFTTVQPPAGVTTTSPTEDSSVELPTPHTPQIVVTILDSNEVIPSLITTTGSPTTHHHHHHHPHHEAEGTTLQPLEEDEHHHHHHHDEFTTPQPVEITTGHPLQTEDLIGVQEPAVVTTESPFAPAETTVVPVVVPATIAPLGTAAPPATPAPVPPATTTPPPSPPSLATETPTLPPTLPPVTLPPVTQPPPTIPPTPPSTQSAQTLPPPTSAINVYTTPDGPPTASQTKPSVTESSEEVEGTNTVSTGGRGSGGVPEEKAGDVDCIKLGCYNGGTCVTTSEGSRCVCRFDRQGPLCELPIIIRNAAFSGDSYVSHRIYKDIGGHESLDAVLPMHIQLKVRTRATNGLIMLAAAQGTKGGHYMALFLQKGLMQFQFSCGLQTMLLSELETPVNTGHEITIRAELDFSRNYTHCNASLLVNDTLAMSGDQPTWLKLLPPRLHTPEAILNTWLHLGGAPQAPIGLIIELPPAQSGSGFTGCLHTLRINGQAREIFGDALDGFGITECGSLACLSSPCRNGAACIKIETNDLDENGEKAEKWKCKCPTGYMGPTCEISVCEDNPCQYGGTCVQFPGSGYLCLCPLGKHGHYCEHNLEVALPSFSGSVNGLSSFVAYTVPIPLEYSLELSFKILPQTMSQISLLAFFGQSGYHDEKSDHLAVSFIQGYIMLTWNLGAGPRRIFTQKPIDFRLDAPRVPYEIKVGRIGRQAWLSVDGKFNITGRSPGSGSRMDVLPILYLGGHEIANFNTLPHDLPLHSGFQGCIYDVQLKAGQVTVPLQETRGVRGRGVGQCGTRECHRHACQHDGACLQHGATFTCICQEGWYGPLCAQPTNPCDSFNNKCYEDATCVPLVNGYECDCPVGRTGKNCEEVIRSLSDVSLTGRRSYLAVRWPYLYDGGDKLGAKRSQMVSYRNFTKKLMPPKPITTPSSHFVMKLLNEVEKQRSFSPVPLMGSKSFEEHHRVQFFFIEFQLRPLSERGLLLYFGTLNNNQDKKIGFVSLSLQGGVVEFRISGPSNHVTVVRSVRMLAIGEWHKIKMAQRGRWLTLWVEGSASSALAPSAEVLVEPDSLLYIGGLKDVSKLPHNAISGFPIPFRGCVRGLVVSGTRIVLNETNIVESRNIRDCDGTACGGDSCESGGHCWLDEKLQPHCICPEYAKGDRCEYSETCKLIPCKNNGRCLRSGRCSCPNGWGGFYCEIAMSKPTTPSFRGNSYLILPPPRIPMKDKRRGPSLYVRPREAIQVSLNFSTIEPDGLLLWSEHERSKFLGLGLEAGHLKLASNLLGSTNDTVRAPASGFIADGAWHWTSVLLDRSRLELQLDGEVIFTERLPEGGRSLGSTTPRSTLAGRRKNSSKEPTISYEDVFYLGGFPNSDSVSRRTKGRFFDPFKGCLQDIQFGAEPTAIISDFSTYQGENIGSCDLHGDEPLTV

Target ORF information:

RefSeq Version	NM_001272995.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eyes shut (eys), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272995.1

ORF Insert Sequence:

ATGAGCAACG TGCATCAGTT TGATACGCAG ACGATGGCGG AATCGCCTCA AATTAGACGC 
GACATGGGTC GCCTATGTGC CACGTGGCCT TCGAAGGACT CTGAGGACGG GGCCGGCACA 
GCTTTGAGGG CAGCCACACC TCTGACAGCC AACGGAGCCA CCACCACCGG GCTGAGTGTT 
ACCCTTGCCC CGAAGGACAT GCAACGAAAC CACCTGCTAA AGATGCCAAC AGCAACAATA 
GAGAAACCAA CGATAACAGC CACCATCGCC AGCAGCAGCA GCACCAGCAC TAGCACCACT 
AGAAAATCAG TTACAGCCAC ACGGAGCCTG AAGTTGAACC CGAACATTCT CCTTCCGACA 
CTAAGGATAT TAGCAAGGGG TCTGCTGCTA CCCGCTCTGA TCCTGGCCAT CCTCGTGGGC 
AGCAGCCAGG CGGGATTTGC CTGCCTCAGC AATCCCTGTG TGTTCGGCGT GTGCATCGAT 
GGCCTAAACA GCTCGTACTC GTGTTACTGC ATTGATGGCT ACACAGGGAT CCAGTGCCAA 
ACGAATTGGG ACGAGTGCTG GTCGAGTCCT TGCCAGAACG GCGGGACATG TGTGGACGGT 
GTGGCCTACT ACAATTGTAC GTGTCCGGAA GGATTTTCTG GATCCAACTG CGAGGAGAAC 
GTCGACGAAT GCATGTCGAA TCCCTGCCAG AACGGCGGAC TCTGCCGGGA CAGAACCAAT 
GGCTACATAT GCACCTGCCA ACCAGGTTAC CTGGGATCTC ACTGCGAACT GGATGTCGCT 
GTCTGCGAGA CAGGAACTGG TGCTCGTTGC CAGCACGGTG GTGAGTGCAT CGAGGGTCCT 
GGACTGGAGT TCACCTGCGA CTGTCCGGCG GGCTGGCATG GTCGGATTTG CCAGGAGGAG 
ATTAACGAGT GCGCCTCGTC GCCCTGCCAA AATGGAGGCG TCTGCGTGGA CAAGCTGGCG 
GCCTATGCCT GCGCCTGTCC CATGGGATAT ACGGGCATCA ACTGCGAGGA GGAGATCCTC 
ATCTGTGCGG ACAATCCGTG TCAGAATAAT GCCTTGTGCT TGATGGAGGA GGGTGTACCC 
ACGTGCTATT GTGTACCCGA CTATCATGGC GAGAAGTGTG AGTTCCAGTA CGACGAATGC 
CAATTGGGAC CGCGGTGCAT GAATGGTGGT GTTTGCATCG ACGGTGTCGA TACCTTCTCC 
TGCTCATGTC CTCCCCTATT GACCGGTATG CTCTGCGAGT GCCTGATGGT GGGTGAGGAG 
TCCCTGGACT GCAACTACAC AGCACCGGCC ACGCAATCTC CGCCACGGAG AACTACCACC 
ACATCCACAA TGGCTCCACC CACAGTGAGG CCCGTCACTC CGCCGGAGAC AACGGTATCG 
CCATCCAGAG CTTCAGAAGA GGTGGAGATT ATAGTAGTTA CCACATCTGC TCCTGCGGAA 
GTGGTCACCT CTGTGCTATC CCCATCATCC TCTTCTTCAT CGTCGGAGGA AGGCGTTTCC 
GTCGAGATTA AAACACCGAC TGTGGCGCCC CCAGAAAGCG GTAGCCATAG CATATCCGTG 
GAGCAAACAA CGGCCGTTCC AGCACAACCC GAACCCGAAT CCGAACAAGA ACCAGAATCT 
AAGCCACATC CCGAATCGGA ATCGGCGTCG GAATCGGAAA CGGAAACGGA GGAGGAAATA 
ATTCCGGGAA CTACAGCGCG ACCACCAACA AGCAGATCCT CCAGTTCTTC GGAGGAATCT 
CCCAGCATCT TCACCACTTT GCCTCCGTTG CCGGGAAAGC CTCAAACGTC TGCGTCGTCG 
GAAAGTTCCG GAGAAGTGGT TACCTCCGAG GAATACACCA CTGTGCCGCA CTTCGAAGTG 
AGTGGCAGCA AAAGTGAAAG TGGTAGTGAA GAGGTCACCA CTGTGCGACC CACTGCAGCA 
CCGAGTATCA CCATTTCAGT GGACATCACC TCATCCGGAA GTAGCAGTAG TAGTTCGGAA 
TCAGTGGAGG TATTCACCAC ACCAGCTCCC GTTTTCGTGC AGAGAGTGAC TACGATCGAA 
ACTAGCATCT CCATAGACTA TGTCACACCC ACTCCTCTGC CGGAAACCAC CACCCCACGG 
GTGGTCCCGG TGCCCAGGCC TACCTTTGCC CCAGAGCCAC CACTGGATGT GGTGGAAACG 
ACTGCCTCAA CTCACCACCT TTGGACAGAG GTGCCCACCA CGGCGGCTCC CTTCTTCACG 
GAGTATCCGG CGGAAGTGCT GATCACTACA CATCGAACGA GTGCTGGCAG ATTCACCACA 
GTTCAACCAC CTGCAGGTGT CACCACCACC TCACCCACTG AGGACTCATC CGTTGAGTTA 
CCCACTCCGC ACACGCCCCA AATTGTGGTC ACCATTTTGG ATTCAAATGA AGTTATACCT 
TCGCTGATCA CAACCACTGG ATCCCCGACA ACACACCATC ACCACCATCA TCATCCTCAC 
CATGAAGCCG AAGGAACCAC TCTTCAGCCT CTCGAAGAAG ACGAGCACCA CCATCATCAT 
CATCATGACG AGTTCACCAC TCCACAGCCA GTGGAAATAA CTACGGGACA TCCTTTGCAA 
ACAGAGGACC TTATTGGAGT GCAGGAACCT GCCGTGGTGA CCACAGAGTC GCCCTTTGCT 
CCAGCTGAGA CAACTGTGGT TCCTGTGGTG GTGCCCGCTA CGATTGCACC ACTTGGAACT 
GCAGCACCAC CAGCCACACC AGCTCCTGTT CCTCCAGCGA CTACCACTCC TCCTCCTTCG 
CCTCCTTCCT TGGCTACAGA GACACCTACA CTGCCACCCA CTTTACCTCC TGTTACCCTG 
CCACCGGTAA CCCAGCCGCC GCCCACGATA CCACCCACTC CTCCATCCAC ACAATCCGCA 
CAAACTTTAC CGCCACCGAC GTCGGCCATA AATGTTTACA CCACTCCCGA TGGACCACCC 
ACGGCTTCCC AAACGAAGCC ATCGGTCACG GAGAGCAGCG AGGAAGTGGA GGGCACAAAT 
ACGGTCTCCA CCGGCGGAAG GGGATCCGGC GGAGTGCCCG AGGAGAAGGC AGGCGATGTC 
GATTGCATCA AGCTGGGCTG CTACAACGGT GGCACCTGCG TAACCACATC CGAAGGATCG 
CGGTGTGTTT GTCGTTTCGA CCGCCAAGGA CCTCTTTGCG AGCTGCCCAT TATCATCCGG 
AATGCCGCCT TCTCCGGCGA CTCCTATGTG TCGCACCGCA TCTACAAGGA CATCGGGGGC 
CACGAGTCCT TGGACGCCGT CCTGCCGATG CACATCCAGC TGAAGGTGCG AACCCGGGCC 
ACCAACGGGC TGATCATGCT GGCGGCGGCG CAGGGCACCA AGGGTGGCCA CTACATGGCC 
CTTTTCCTGC AGAAGGGACT CATGCAGTTC CAGTTCTCCT GCGGACTGCA GACGATGCTG 
CTGAGTGAAC TGGAAACGCC GGTCAACACC GGCCACGAAA TCACCATTCG AGCTGAATTG 
GACTTCAGCC GGAATTACAC ACACTGCAAC GCCTCGTTGC TGGTGAACGA CACGTTGGCC 
ATGTCCGGGG ATCAGCCCAC CTGGCTGAAG CTACTACCGC CGCGCCTTCA CACCCCGGAG 
GCCATCCTCA ACACCTGGCT GCATCTGGGC GGAGCGCCCC AGGCGCCGAT TGGCCTGATT 
ATCGAACTGC CGCCGGCCCA AAGTGGAAGC GGCTTCACCG GCTGCCTCCA TACGCTGCGC 
ATCAATGGAC AGGCTCGCGA GATTTTCGGC GATGCCCTCG ATGGCTTTGG CATTACGGAA 
TGCGGCTCAT TGGCGTGTCT ATCGAGTCCT TGCCGCAATG GCGCCGCCTG CATAAAGATC 
GAGACCAACG ATCTGGACGA GAATGGCGAG AAGGCGGAGA AGTGGAAGTG CAAGTGCCCC 
ACCGGCTACA TGGGCCCCAC CTGCGAGATA TCCGTGTGCG AGGACAACCC GTGCCAGTAC 
GGTGGAACCT GCGTACAATT TCCGGGCAGC GGTTACCTCT GCCTGTGTCC GCTGGGCAAG 
CACGGCCACT ATTGCGAGCA CAATCTTGAA GTGGCCCTGC CATCCTTCTC CGGCAGCGTC 
AATGGACTCT CCTCGTTTGT GGCCTACACG GTGCCGATTC CCCTCGAGTA TTCGCTCGAG 
CTGAGCTTCA AAATCCTGCC GCAGACCATG TCTCAGATTT CGCTGCTGGC CTTCTTCGGG 
CAGTCGGGTT ATCACGACGA GAAGAGCGAT CACCTGGCGG TGAGCTTCAT CCAGGGCTAC 
ATAATGCTCA CCTGGAATCT GGGAGCCGGG CCCCGTCGCA TATTCACACA GAAACCCATT 
GATTTCCGGC TGGATGCTCC CCGGGTTCCG TACGAGATTA AGGTGGGCCG GATTGGAAGA 
CAGGCCTGGC TCTCGGTGGA TGGAAAGTTC AATATTACGG GTCGATCGCC CGGAAGTGGC 
AGTCGGATGG ATGTGTTGCC TATTCTGTAT CTCGGCGGTC ACGAGATAGC CAATTTCAAT 
ACGCTGCCAC ACGATTTGCC ACTGCATTCG GGATTCCAGG GATGCATATA CGATGTACAG 
CTGAAGGCGG GACAGGTCAC GGTGCCCCTC CAGGAGACGC GCGGGGTCAG AGGTCGGGGC 
GTGGGTCAAT GTGGAACCAG GGAATGCCAT CGCCATGCCT GCCAGCACGA TGGCGCCTGC 
CTGCAGCATG GAGCAACCTT TACTTGCATC TGCCAGGAGG GCTGGTATGG GCCACTGTGT 
GCCCAGCCCA CGAATCCCTG TGACTCCTTC AACAACAAAT GCTACGAGGA CGCCACCTGT 
GTGCCGCTGG TCAATGGCTA CGAATGCGAT TGTCCTGTAG GACGCACAGG CAAAAACTGC 
GAGGAGGTAA TTCGATCCCT GAGCGACGTA TCCCTGACTG GCAGACGATC CTATCTGGCG 
GTTCGCTGGC CATATCTATA CGATGGCGGG GATAAACTGG GCGCTAAGCG CTCCCAAATG 
GTCAGCTACC GGAACTTCAC CAAGAAACTA ATGCCCCCGA AGCCCATTAC CACGCCCAGC 
AGCCACTTTG TGATGAAACT GCTGAATGAG GTGGAGAAGC AGCGCAGCTT CTCGCCAGTT 
CCCCTGATGG GATCGAAGTC CTTCGAGGAG CACCATCGAG TGCAGTTCTT CTTCATCGAG 
TTCCAACTGC GCCCGCTCTC GGAAAGAGGC CTCCTTCTGT ACTTCGGTAC CCTGAACAAC 
AATCAGGACA AGAAGATAGG ATTCGTGTCG CTATCGCTGC AGGGCGGAGT GGTGGAGTTC 
CGGATTTCCG GGCCCAGCAA TCATGTCACG GTGGTGCGGA GCGTTCGCAT GTTGGCCATC 
GGCGAGTGGC ACAAGATCAA GATGGCGCAG CGCGGAAGAT GGCTGACCCT GTGGGTGGAG 
GGTAGCGCCT CGTCGGCTTT GGCTCCTTCG GCTGAGGTGC TCGTCGAGCC CGACTCACTG 
CTCTACATTG GCGGGCTGAA GGATGTGTCC AAGCTGCCGC ACAACGCCAT CTCCGGTTTC 
CCGATTCCGT TCAGGGGATG TGTCCGGGGT CTGGTGGTCA GTGGAACTCG CATTGTGCTC 
AACGAAACCA ACATTGTGGA ATCGCGCAAC ATTCGAGACT GTGATGGCAC CGCTTGTGGC 
GGAGACTCCT GCGAGTCCGG AGGACATTGC TGGCTGGACG AGAAGCTGCA GCCGCATTGC 
ATCTGTCCGG AGTACGCGAA AGGAGATCGA TGCGAGTACT CGGAGACCTG CAAGCTCATT 
CCGTGCAAAA ACAATGGAAG ATGCCTGAGA AGTGGACGCT GCTCGTGTCC AAATGGATGG 
GGTGGCTTCT ACTGCGAGAT TGCCATGAGC AAGCCGACGA CGCCGAGCTT CCGTGGCAAC 
AGTTACCTGA TCTTGCCGCC GCCGCGAATT CCGATGAAAG ACAAGCGGCG GGGACCCTCC 
CTGTACGTCC GCCCCCGCGA AGCCATCCAA GTCTCGCTGA ACTTCTCCAC CATCGAACCG 
GACGGACTGC TCCTGTGGAG CGAGCACGAG AGGAGCAAGT TCCTCGGCCT GGGCCTGGAG 
GCGGGCCACC TCAAGCTGGC CAGCAACCTC CTGGGCAGCA CCAACGACAC GGTCCGTGCT 
CCGGCCAGCG GATTCATAGC CGACGGAGCC TGGCACTGGA CCAGCGTCCT GCTGGACCGG 
TCCAGGCTGG AGCTGCAGCT GGACGGCGAG GTGATCTTCA CGGAGCGATT GCCCGAGGGC 
GGACGGTCAC TGGGCTCGAC CACGCCGAGA AGCACTCTGG CGGGCAGGCG CAAGAATTCG 
AGCAAGGAGC CAACAATTAG CTACGAGGAT GTCTTCTACT TGGGCGGCTT TCCCAACTCG 
GACTCGGTGA GCAGGCGCAC AAAGGGTCGC TTCTTCGATC CCTTCAAGGG CTGCCTGCAG 
GATATCCAGT TCGGAGCCGA GCCCACAGCG ATTATCAGCG ACTTCTCGAC GTACCAGGGC 
GAGAACATTG GATCCTGTGA TCTGCACGGC GATGAGCCGC TTACTGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eys ( NM_001032399.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001027571.3 Drosophila melanogaster eyes shut (eys), transcript variant F, mRNA.

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CloneID	OFa22383
Clone ID Related Accession (Same CDS sequence)	NM_001272995.1 , NM_001032399.3
Accession Version	NM_001032399.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eyes shut, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001032399.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCAACG TGCATCAGTT TGATACGCAG ACGATGGCGG AATCGCCTCA AATTAGACGC 
GACATGGGTC GCCTATGTGC CACGTGGCCT TCGAAGGACT CTGAGGACGG GGCCGGCACA 
GCTTTGAGGG CAGCCACACC TCTGACAGCC AACGGAGCCA CCACCACCGG GCTGAGTGTT 
ACCCTTGCCC CGAAGGACAT GCAACGAAAC CACCTGCTAA AGATGCCAAC AGCAACAATA 
GAGAAACCAA CGATAACAGC CACCATCGCC AGCAGCAGCA GCACCAGCAC TAGCACCACT 
AGAAAATCAG TTACAGCCAC ACGGAGCCTG AAGTTGAACC CGAACATTCT CCTTCCGACA 
CTAAGGATAT TAGCAAGGGG TCTGCTGCTA CCCGCTCTGA TCCTGGCCAT CCTCGTGGGC 
AGCAGCCAGG CGGGATTTGC CTGCCTCAGC AATCCCTGTG TGTTCGGCGT GTGCATCGAT 
GGCCTAAACA GCTCGTACTC GTGTTACTGC ATTGATGGCT ACACAGGGAT CCAGTGCCAA 
ACGAATTGGG ACGAGTGCTG GTCGAGTCCT TGCCAGAACG GCGGGACATG TGTGGACGGT 
GTGGCCTACT ACAATTGTAC GTGTCCGGAA GGATTTTCTG GATCCAACTG CGAGGAGAAC 
GTCGACGAAT GCATGTCGAA TCCCTGCCAG AACGGCGGAC TCTGCCGGGA CAGAACCAAT 
GGCTACATAT GCACCTGCCA ACCAGGTTAC CTGGGATCTC ACTGCGAACT GGATGTCGCT 
GTCTGCGAGA CAGGAACTGG TGCTCGTTGC CAGCACGGTG GTGAGTGCAT CGAGGGTCCT 
GGACTGGAGT TCACCTGCGA CTGTCCGGCG GGCTGGCATG GTCGGATTTG CCAGGAGGAG 
ATTAACGAGT GCGCCTCGTC GCCCTGCCAA AATGGAGGCG TCTGCGTGGA CAAGCTGGCG 
GCCTATGCCT GCGCCTGTCC CATGGGATAT ACGGGCATCA ACTGCGAGGA GGAGATCCTC 
ATCTGTGCGG ACAATCCGTG TCAGAATAAT GCCTTGTGCT TGATGGAGGA GGGTGTACCC 
ACGTGCTATT GTGTACCCGA CTATCATGGC GAGAAGTGTG AGTTCCAGTA CGACGAATGC 
CAATTGGGAC CGCGGTGCAT GAATGGTGGT GTTTGCATCG ACGGTGTCGA TACCTTCTCC 
TGCTCATGTC CTCCCCTATT GACCGGTATG CTCTGCGAGT GCCTGATGGT GGGTGAGGAG 
TCCCTGGACT GCAACTACAC AGCACCGGCC ACGCAATCTC CGCCACGGAG AACTACCACC 
ACATCCACAA TGGCTCCACC CACAGTGAGG CCCGTCACTC CGCCGGAGAC AACGGTATCG 
CCATCCAGAG CTTCAGAAGA GGTGGAGATT ATAGTAGTTA CCACATCTGC TCCTGCGGAA 
GTGGTCACCT CTGTGCTATC CCCATCATCC TCTTCTTCAT CGTCGGAGGA AGGCGTTTCC 
GTCGAGATTA AAACACCGAC TGTGGCGCCC CCAGAAAGCG GTAGCCATAG CATATCCGTG 
GAGCAAACAA CGGCCGTTCC AGCACAACCC GAACCCGAAT CCGAACAAGA ACCAGAATCT 
AAGCCACATC CCGAATCGGA ATCGGCGTCG GAATCGGAAA CGGAAACGGA GGAGGAAATA 
ATTCCGGGAA CTACAGCGCG ACCACCAACA AGCAGATCCT CCAGTTCTTC GGAGGAATCT 
CCCAGCATCT TCACCACTTT GCCTCCGTTG CCGGGAAAGC CTCAAACGTC TGCGTCGTCG 
GAAAGTTCCG GAGAAGTGGT TACCTCCGAG GAATACACCA CTGTGCCGCA CTTCGAAGTG 
AGTGGCAGCA AAAGTGAAAG TGGTAGTGAA GAGGTCACCA CTGTGCGACC CACTGCAGCA 
CCGAGTATCA CCATTTCAGT GGACATCACC TCATCCGGAA GTAGCAGTAG TAGTTCGGAA 
TCAGTGGAGG TATTCACCAC ACCAGCTCCC GTTTTCGTGC AGAGAGTGAC TACGATCGAA 
ACTAGCATCT CCATAGACTA TGTCACACCC ACTCCTCTGC CGGAAACCAC CACCCCACGG 
GTGGTCCCGG TGCCCAGGCC TACCTTTGCC CCAGAGCCAC CACTGGATGT GGTGGAAACG 
ACTGCCTCAA CTCACCACCT TTGGACAGAG GTGCCCACCA CGGCGGCTCC CTTCTTCACG 
GAGTATCCGG CGGAAGTGCT GATCACTACA CATCGAACGA GTGCTGGCAG ATTCACCACA 
GTTCAACCAC CTGCAGGTGT CACCACCACC TCACCCACTG AGGACTCATC CGTTGAGTTA 
CCCACTCCGC ACACGCCCCA AATTGTGGTC ACCATTTTGG ATTCAAATGA AGTTATACCT 
TCGCTGATCA CAACCACTGG ATCCCCGACA ACACACCATC ACCACCATCA TCATCCTCAC 
CATGAAGCCG AAGGAACCAC TCTTCAGCCT CTCGAAGAAG ACGAGCACCA CCATCATCAT 
CATCATGACG AGTTCACCAC TCCACAGCCA GTGGAAATAA CTACGGGACA TCCTTTGCAA 
ACAGAGGACC TTATTGGAGT GCAGGAACCT GCCGTGGTGA CCACAGAGTC GCCCTTTGCT 
CCAGCTGAGA CAACTGTGGT TCCTGTGGTG GTGCCCGCTA CGATTGCACC ACTTGGAACT 
GCAGCACCAC CAGCCACACC AGCTCCTGTT CCTCCAGCGA CTACCACTCC TCCTCCTTCG 
CCTCCTTCCT TGGCTACAGA GACACCTACA CTGCCACCCA CTTTACCTCC TGTTACCCTG 
CCACCGGTAA CCCAGCCGCC GCCCACGATA CCACCCACTC CTCCATCCAC ACAATCCGCA 
CAAACTTTAC CGCCACCGAC GTCGGCCATA AATGTTTACA CCACTCCCGA TGGACCACCC 
ACGGCTTCCC AAACGAAGCC ATCGGTCACG GAGAGCAGCG AGGAAGTGGA GGGCACAAAT 
ACGGTCTCCA CCGGCGGAAG GGGATCCGGC GGAGTGCCCG AGGAGAAGGC AGGCGATGTC 
GATTGCATCA AGCTGGGCTG CTACAACGGT GGCACCTGCG TAACCACATC CGAAGGATCG 
CGGTGTGTTT GTCGTTTCGA CCGCCAAGGA CCTCTTTGCG AGCTGCCCAT TATCATCCGG 
AATGCCGCCT TCTCCGGCGA CTCCTATGTG TCGCACCGCA TCTACAAGGA CATCGGGGGC 
CACGAGTCCT TGGACGCCGT CCTGCCGATG CACATCCAGC TGAAGGTGCG AACCCGGGCC 
ACCAACGGGC TGATCATGCT GGCGGCGGCG CAGGGCACCA AGGGTGGCCA CTACATGGCC 
CTTTTCCTGC AGAAGGGACT CATGCAGTTC CAGTTCTCCT GCGGACTGCA GACGATGCTG 
CTGAGTGAAC TGGAAACGCC GGTCAACACC GGCCACGAAA TCACCATTCG AGCTGAATTG 
GACTTCAGCC GGAATTACAC ACACTGCAAC GCCTCGTTGC TGGTGAACGA CACGTTGGCC 
ATGTCCGGGG ATCAGCCCAC CTGGCTGAAG CTACTACCGC CGCGCCTTCA CACCCCGGAG 
GCCATCCTCA ACACCTGGCT GCATCTGGGC GGAGCGCCCC AGGCGCCGAT TGGCCTGATT 
ATCGAACTGC CGCCGGCCCA AAGTGGAAGC GGCTTCACCG GCTGCCTCCA TACGCTGCGC 
ATCAATGGAC AGGCTCGCGA GATTTTCGGC GATGCCCTCG ATGGCTTTGG CATTACGGAA 
TGCGGCTCAT TGGCGTGTCT ATCGAGTCCT TGCCGCAATG GCGCCGCCTG CATAAAGATC 
GAGACCAACG ATCTGGACGA GAATGGCGAG AAGGCGGAGA AGTGGAAGTG CAAGTGCCCC 
ACCGGCTACA TGGGCCCCAC CTGCGAGATA TCCGTGTGCG AGGACAACCC GTGCCAGTAC 
GGTGGAACCT GCGTACAATT TCCGGGCAGC GGTTACCTCT GCCTGTGTCC GCTGGGCAAG 
CACGGCCACT ATTGCGAGCA CAATCTTGAA GTGGCCCTGC CATCCTTCTC CGGCAGCGTC 
AATGGACTCT CCTCGTTTGT GGCCTACACG GTGCCGATTC CCCTCGAGTA TTCGCTCGAG 
CTGAGCTTCA AAATCCTGCC GCAGACCATG TCTCAGATTT CGCTGCTGGC CTTCTTCGGG 
CAGTCGGGTT ATCACGACGA GAAGAGCGAT CACCTGGCGG TGAGCTTCAT CCAGGGCTAC 
ATAATGCTCA CCTGGAATCT GGGAGCCGGG CCCCGTCGCA TATTCACACA GAAACCCATT 
GATTTCCGGC TGGATGCTCC CCGGGTTCCG TACGAGATTA AGGTGGGCCG GATTGGAAGA 
CAGGCCTGGC TCTCGGTGGA TGGAAAGTTC AATATTACGG GTCGATCGCC CGGAAGTGGC 
AGTCGGATGG ATGTGTTGCC TATTCTGTAT CTCGGCGGTC ACGAGATAGC CAATTTCAAT 
ACGCTGCCAC ACGATTTGCC ACTGCATTCG GGATTCCAGG GATGCATATA CGATGTACAG 
CTGAAGGCGG GACAGGTCAC GGTGCCCCTC CAGGAGACGC GCGGGGTCAG AGGTCGGGGC 
GTGGGTCAAT GTGGAACCAG GGAATGCCAT CGCCATGCCT GCCAGCACGA TGGCGCCTGC 
CTGCAGCATG GAGCAACCTT TACTTGCATC TGCCAGGAGG GCTGGTATGG GCCACTGTGT 
GCCCAGCCCA CGAATCCCTG TGACTCCTTC AACAACAAAT GCTACGAGGA CGCCACCTGT 
GTGCCGCTGG TCAATGGCTA CGAATGCGAT TGTCCTGTAG GACGCACAGG CAAAAACTGC 
GAGGAGGTAA TTCGATCCCT GAGCGACGTA TCCCTGACTG GCAGACGATC CTATCTGGCG 
GTTCGCTGGC CATATCTATA CGATGGCGGG GATAAACTGG GCGCTAAGCG CTCCCAAATG 
GTCAGCTACC GGAACTTCAC CAAGAAACTA ATGCCCCCGA AGCCCATTAC CACGCCCAGC 
AGCCACTTTG TGATGAAACT GCTGAATGAG GTGGAGAAGC AGCGCAGCTT CTCGCCAGTT 
CCCCTGATGG GATCGAAGTC CTTCGAGGAG CACCATCGAG TGCAGTTCTT CTTCATCGAG 
TTCCAACTGC GCCCGCTCTC GGAAAGAGGC CTCCTTCTGT ACTTCGGTAC CCTGAACAAC 
AATCAGGACA AGAAGATAGG ATTCGTGTCG CTATCGCTGC AGGGCGGAGT GGTGGAGTTC 
CGGATTTCCG GGCCCAGCAA TCATGTCACG GTGGTGCGGA GCGTTCGCAT GTTGGCCATC 
GGCGAGTGGC ACAAGATCAA GATGGCGCAG CGCGGAAGAT GGCTGACCCT GTGGGTGGAG 
GGTAGCGCCT CGTCGGCTTT GGCTCCTTCG GCTGAGGTGC TCGTCGAGCC CGACTCACTG 
CTCTACATTG GCGGGCTGAA GGATGTGTCC AAGCTGCCGC ACAACGCCAT CTCCGGTTTC 
CCGATTCCGT TCAGGGGATG TGTCCGGGGT CTGGTGGTCA GTGGAACTCG CATTGTGCTC 
AACGAAACCA ACATTGTGGA ATCGCGCAAC ATTCGAGACT GTGATGGCAC CGCTTGTGGC 
GGAGACTCCT GCGAGTCCGG AGGACATTGC TGGCTGGACG AGAAGCTGCA GCCGCATTGC 
ATCTGTCCGG AGTACGCGAA AGGAGATCGA TGCGAGTACT CGGAGACCTG CAAGCTCATT 
CCGTGCAAAA ACAATGGAAG ATGCCTGAGA AGTGGACGCT GCTCGTGTCC AAATGGATGG 
GGTGGCTTCT ACTGCGAGAT TGCCATGAGC AAGCCGACGA CGCCGAGCTT CCGTGGCAAC 
AGTTACCTGA TCTTGCCGCC GCCGCGAATT CCGATGAAAG ACAAGCGGCG GGGACCCTCC 
CTGTACGTCC GCCCCCGCGA AGCCATCCAA GTCTCGCTGA ACTTCTCCAC CATCGAACCG 
GACGGACTGC TCCTGTGGAG CGAGCACGAG AGGAGCAAGT TCCTCGGCCT GGGCCTGGAG 
GCGGGCCACC TCAAGCTGGC CAGCAACCTC CTGGGCAGCA CCAACGACAC GGTCCGTGCT 
CCGGCCAGCG GATTCATAGC CGACGGAGCC TGGCACTGGA CCAGCGTCCT GCTGGACCGG 
TCCAGGCTGG AGCTGCAGCT GGACGGCGAG GTGATCTTCA CGGAGCGATT GCCCGAGGGC 
GGACGGTCAC TGGGCTCGAC CACGCCGAGA AGCACTCTGG CGGGCAGGCG CAAGAATTCG 
AGCAAGGAGC CAACAATTAG CTACGAGGAT GTCTTCTACT TGGGCGGCTT TCCCAACTCG 
GACTCGGTGA GCAGGCGCAC AAAGGGTCGC TTCTTCGATC CCTTCAAGGG CTGCCTGCAG 
GATATCCAGT TCGGAGCCGA GCCCACAGCG ATTATCAGCG ACTTCTCGAC GTACCAGGGC 
GAGAACATTG GATCCTGTGA TCTGCACGGC GATGAGCCGC TTACTGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001027571.3
CDS	884..7414
Translation	MSNVHQFDTQTMAESPQIRRDMGRLCATWPSKDSEDGAGTALRAATPLTANGATTTGLSVTLAPKDMQRNHLLKMPTATIEKPTITATIASSSSTSTSTTRKSVTATRSLKLNPNILLPTLRILARGLLLPALILAILVGSSQAGFACLSNPCVFGVCIDGLNSSYSCYCIDGYTGIQCQTNWDECWSSPCQNGGTCVDGVAYYNCTCPEGFSGSNCEENVDECMSNPCQNGGLCRDRTNGYICTCQPGYLGSHCELDVAVCETGTGARCQHGGECIEGPGLEFTCDCPAGWHGRICQEEINECASSPCQNGGVCVDKLAAYACACPMGYTGINCEEEILICADNPCQNNALCLMEEGVPTCYCVPDYHGEKCEFQYDECQLGPRCMNGGVCIDGVDTFSCSCPPLLTGMLCECLMVGEESLDCNYTAPATQSPPRRTTTTSTMAPPTVRPVTPPETTVSPSRASEEVEIIVVTTSAPAEVVTSVLSPSSSSSSSEEGVSVEIKTPTVAPPESGSHSISVEQTTAVPAQPEPESEQEPESKPHPESESASESETETEEEIIPGTTARPPTSRSSSSSEESPSIFTTLPPLPGKPQTSASSESSGEVVTSEEYTTVPHFEVSGSKSESGSEEVTTVRPTAAPSITISVDITSSGSSSSSSESVEVFTTPAPVFVQRVTTIETSISIDYVTPTPLPETTTPRVVPVPRPTFAPEPPLDVVETTASTHHLWTEVPTTAAPFFTEYPAEVLITTHRTSAGRFTTVQPPAGVTTTSPTEDSSVELPTPHTPQIVVTILDSNEVIPSLITTTGSPTTHHHHHHHPHHEAEGTTLQPLEEDEHHHHHHHDEFTTPQPVEITTGHPLQTEDLIGVQEPAVVTTESPFAPAETTVVPVVVPATIAPLGTAAPPATPAPVPPATTTPPPSPPSLATETPTLPPTLPPVTLPPVTQPPPTIPPTPPSTQSAQTLPPPTSAINVYTTPDGPPTASQTKPSVTESSEEVEGTNTVSTGGRGSGGVPEEKAGDVDCIKLGCYNGGTCVTTSEGSRCVCRFDRQGPLCELPIIIRNAAFSGDSYVSHRIYKDIGGHESLDAVLPMHIQLKVRTRATNGLIMLAAAQGTKGGHYMALFLQKGLMQFQFSCGLQTMLLSELETPVNTGHEITIRAELDFSRNYTHCNASLLVNDTLAMSGDQPTWLKLLPPRLHTPEAILNTWLHLGGAPQAPIGLIIELPPAQSGSGFTGCLHTLRINGQAREIFGDALDGFGITECGSLACLSSPCRNGAACIKIETNDLDENGEKAEKWKCKCPTGYMGPTCEISVCEDNPCQYGGTCVQFPGSGYLCLCPLGKHGHYCEHNLEVALPSFSGSVNGLSSFVAYTVPIPLEYSLELSFKILPQTMSQISLLAFFGQSGYHDEKSDHLAVSFIQGYIMLTWNLGAGPRRIFTQKPIDFRLDAPRVPYEIKVGRIGRQAWLSVDGKFNITGRSPGSGSRMDVLPILYLGGHEIANFNTLPHDLPLHSGFQGCIYDVQLKAGQVTVPLQETRGVRGRGVGQCGTRECHRHACQHDGACLQHGATFTCICQEGWYGPLCAQPTNPCDSFNNKCYEDATCVPLVNGYECDCPVGRTGKNCEEVIRSLSDVSLTGRRSYLAVRWPYLYDGGDKLGAKRSQMVSYRNFTKKLMPPKPITTPSSHFVMKLLNEVEKQRSFSPVPLMGSKSFEEHHRVQFFFIEFQLRPLSERGLLLYFGTLNNNQDKKIGFVSLSLQGGVVEFRISGPSNHVTVVRSVRMLAIGEWHKIKMAQRGRWLTLWVEGSASSALAPSAEVLVEPDSLLYIGGLKDVSKLPHNAISGFPIPFRGCVRGLVVSGTRIVLNETNIVESRNIRDCDGTACGGDSCESGGHCWLDEKLQPHCICPEYAKGDRCEYSETCKLIPCKNNGRCLRSGRCSCPNGWGGFYCEIAMSKPTTPSFRGNSYLILPPPRIPMKDKRRGPSLYVRPREAIQVSLNFSTIEPDGLLLWSEHERSKFLGLGLEAGHLKLASNLLGSTNDTVRAPASGFIADGAWHWTSVLLDRSRLELQLDGEVIFTERLPEGGRSLGSTTPRSTLAGRRKNSSKEPTISYEDVFYLGGFPNSDSVSRRTKGRFFDPFKGCLQDIQFGAEPTAIISDFSTYQGENIGSCDLHGDEPLTV

Target ORF information:

RefSeq Version	NM_001032399.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eyes shut (eys), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001032399.3

ORF Insert Sequence:

ATGAGCAACG TGCATCAGTT TGATACGCAG ACGATGGCGG AATCGCCTCA AATTAGACGC 
GACATGGGTC GCCTATGTGC CACGTGGCCT TCGAAGGACT CTGAGGACGG GGCCGGCACA 
GCTTTGAGGG CAGCCACACC TCTGACAGCC AACGGAGCCA CCACCACCGG GCTGAGTGTT 
ACCCTTGCCC CGAAGGACAT GCAACGAAAC CACCTGCTAA AGATGCCAAC AGCAACAATA 
GAGAAACCAA CGATAACAGC CACCATCGCC AGCAGCAGCA GCACCAGCAC TAGCACCACT 
AGAAAATCAG TTACAGCCAC ACGGAGCCTG AAGTTGAACC CGAACATTCT CCTTCCGACA 
CTAAGGATAT TAGCAAGGGG TCTGCTGCTA CCCGCTCTGA TCCTGGCCAT CCTCGTGGGC 
AGCAGCCAGG CGGGATTTGC CTGCCTCAGC AATCCCTGTG TGTTCGGCGT GTGCATCGAT 
GGCCTAAACA GCTCGTACTC GTGTTACTGC ATTGATGGCT ACACAGGGAT CCAGTGCCAA 
ACGAATTGGG ACGAGTGCTG GTCGAGTCCT TGCCAGAACG GCGGGACATG TGTGGACGGT 
GTGGCCTACT ACAATTGTAC GTGTCCGGAA GGATTTTCTG GATCCAACTG CGAGGAGAAC 
GTCGACGAAT GCATGTCGAA TCCCTGCCAG AACGGCGGAC TCTGCCGGGA CAGAACCAAT 
GGCTACATAT GCACCTGCCA ACCAGGTTAC CTGGGATCTC ACTGCGAACT GGATGTCGCT 
GTCTGCGAGA CAGGAACTGG TGCTCGTTGC CAGCACGGTG GTGAGTGCAT CGAGGGTCCT 
GGACTGGAGT TCACCTGCGA CTGTCCGGCG GGCTGGCATG GTCGGATTTG CCAGGAGGAG 
ATTAACGAGT GCGCCTCGTC GCCCTGCCAA AATGGAGGCG TCTGCGTGGA CAAGCTGGCG 
GCCTATGCCT GCGCCTGTCC CATGGGATAT ACGGGCATCA ACTGCGAGGA GGAGATCCTC 
ATCTGTGCGG ACAATCCGTG TCAGAATAAT GCCTTGTGCT TGATGGAGGA GGGTGTACCC 
ACGTGCTATT GTGTACCCGA CTATCATGGC GAGAAGTGTG AGTTCCAGTA CGACGAATGC 
CAATTGGGAC CGCGGTGCAT GAATGGTGGT GTTTGCATCG ACGGTGTCGA TACCTTCTCC 
TGCTCATGTC CTCCCCTATT GACCGGTATG CTCTGCGAGT GCCTGATGGT GGGTGAGGAG 
TCCCTGGACT GCAACTACAC AGCACCGGCC ACGCAATCTC CGCCACGGAG AACTACCACC 
ACATCCACAA TGGCTCCACC CACAGTGAGG CCCGTCACTC CGCCGGAGAC AACGGTATCG 
CCATCCAGAG CTTCAGAAGA GGTGGAGATT ATAGTAGTTA CCACATCTGC TCCTGCGGAA 
GTGGTCACCT CTGTGCTATC CCCATCATCC TCTTCTTCAT CGTCGGAGGA AGGCGTTTCC 
GTCGAGATTA AAACACCGAC TGTGGCGCCC CCAGAAAGCG GTAGCCATAG CATATCCGTG 
GAGCAAACAA CGGCCGTTCC AGCACAACCC GAACCCGAAT CCGAACAAGA ACCAGAATCT 
AAGCCACATC CCGAATCGGA ATCGGCGTCG GAATCGGAAA CGGAAACGGA GGAGGAAATA 
ATTCCGGGAA CTACAGCGCG ACCACCAACA AGCAGATCCT CCAGTTCTTC GGAGGAATCT 
CCCAGCATCT TCACCACTTT GCCTCCGTTG CCGGGAAAGC CTCAAACGTC TGCGTCGTCG 
GAAAGTTCCG GAGAAGTGGT TACCTCCGAG GAATACACCA CTGTGCCGCA CTTCGAAGTG 
AGTGGCAGCA AAAGTGAAAG TGGTAGTGAA GAGGTCACCA CTGTGCGACC CACTGCAGCA 
CCGAGTATCA CCATTTCAGT GGACATCACC TCATCCGGAA GTAGCAGTAG TAGTTCGGAA 
TCAGTGGAGG TATTCACCAC ACCAGCTCCC GTTTTCGTGC AGAGAGTGAC TACGATCGAA 
ACTAGCATCT CCATAGACTA TGTCACACCC ACTCCTCTGC CGGAAACCAC CACCCCACGG 
GTGGTCCCGG TGCCCAGGCC TACCTTTGCC CCAGAGCCAC CACTGGATGT GGTGGAAACG 
ACTGCCTCAA CTCACCACCT TTGGACAGAG GTGCCCACCA CGGCGGCTCC CTTCTTCACG 
GAGTATCCGG CGGAAGTGCT GATCACTACA CATCGAACGA GTGCTGGCAG ATTCACCACA 
GTTCAACCAC CTGCAGGTGT CACCACCACC TCACCCACTG AGGACTCATC CGTTGAGTTA 
CCCACTCCGC ACACGCCCCA AATTGTGGTC ACCATTTTGG ATTCAAATGA AGTTATACCT 
TCGCTGATCA CAACCACTGG ATCCCCGACA ACACACCATC ACCACCATCA TCATCCTCAC 
CATGAAGCCG AAGGAACCAC TCTTCAGCCT CTCGAAGAAG ACGAGCACCA CCATCATCAT 
CATCATGACG AGTTCACCAC TCCACAGCCA GTGGAAATAA CTACGGGACA TCCTTTGCAA 
ACAGAGGACC TTATTGGAGT GCAGGAACCT GCCGTGGTGA CCACAGAGTC GCCCTTTGCT 
CCAGCTGAGA CAACTGTGGT TCCTGTGGTG GTGCCCGCTA CGATTGCACC ACTTGGAACT 
GCAGCACCAC CAGCCACACC AGCTCCTGTT CCTCCAGCGA CTACCACTCC TCCTCCTTCG 
CCTCCTTCCT TGGCTACAGA GACACCTACA CTGCCACCCA CTTTACCTCC TGTTACCCTG 
CCACCGGTAA CCCAGCCGCC GCCCACGATA CCACCCACTC CTCCATCCAC ACAATCCGCA 
CAAACTTTAC CGCCACCGAC GTCGGCCATA AATGTTTACA CCACTCCCGA TGGACCACCC 
ACGGCTTCCC AAACGAAGCC ATCGGTCACG GAGAGCAGCG AGGAAGTGGA GGGCACAAAT 
ACGGTCTCCA CCGGCGGAAG GGGATCCGGC GGAGTGCCCG AGGAGAAGGC AGGCGATGTC 
GATTGCATCA AGCTGGGCTG CTACAACGGT GGCACCTGCG TAACCACATC CGAAGGATCG 
CGGTGTGTTT GTCGTTTCGA CCGCCAAGGA CCTCTTTGCG AGCTGCCCAT TATCATCCGG 
AATGCCGCCT TCTCCGGCGA CTCCTATGTG TCGCACCGCA TCTACAAGGA CATCGGGGGC 
CACGAGTCCT TGGACGCCGT CCTGCCGATG CACATCCAGC TGAAGGTGCG AACCCGGGCC 
ACCAACGGGC TGATCATGCT GGCGGCGGCG CAGGGCACCA AGGGTGGCCA CTACATGGCC 
CTTTTCCTGC AGAAGGGACT CATGCAGTTC CAGTTCTCCT GCGGACTGCA GACGATGCTG 
CTGAGTGAAC TGGAAACGCC GGTCAACACC GGCCACGAAA TCACCATTCG AGCTGAATTG 
GACTTCAGCC GGAATTACAC ACACTGCAAC GCCTCGTTGC TGGTGAACGA CACGTTGGCC 
ATGTCCGGGG ATCAGCCCAC CTGGCTGAAG CTACTACCGC CGCGCCTTCA CACCCCGGAG 
GCCATCCTCA ACACCTGGCT GCATCTGGGC GGAGCGCCCC AGGCGCCGAT TGGCCTGATT 
ATCGAACTGC CGCCGGCCCA AAGTGGAAGC GGCTTCACCG GCTGCCTCCA TACGCTGCGC 
ATCAATGGAC AGGCTCGCGA GATTTTCGGC GATGCCCTCG ATGGCTTTGG CATTACGGAA 
TGCGGCTCAT TGGCGTGTCT ATCGAGTCCT TGCCGCAATG GCGCCGCCTG CATAAAGATC 
GAGACCAACG ATCTGGACGA GAATGGCGAG AAGGCGGAGA AGTGGAAGTG CAAGTGCCCC 
ACCGGCTACA TGGGCCCCAC CTGCGAGATA TCCGTGTGCG AGGACAACCC GTGCCAGTAC 
GGTGGAACCT GCGTACAATT TCCGGGCAGC GGTTACCTCT GCCTGTGTCC GCTGGGCAAG 
CACGGCCACT ATTGCGAGCA CAATCTTGAA GTGGCCCTGC CATCCTTCTC CGGCAGCGTC 
AATGGACTCT CCTCGTTTGT GGCCTACACG GTGCCGATTC CCCTCGAGTA TTCGCTCGAG 
CTGAGCTTCA AAATCCTGCC GCAGACCATG TCTCAGATTT CGCTGCTGGC CTTCTTCGGG 
CAGTCGGGTT ATCACGACGA GAAGAGCGAT CACCTGGCGG TGAGCTTCAT CCAGGGCTAC 
ATAATGCTCA CCTGGAATCT GGGAGCCGGG CCCCGTCGCA TATTCACACA GAAACCCATT 
GATTTCCGGC TGGATGCTCC CCGGGTTCCG TACGAGATTA AGGTGGGCCG GATTGGAAGA 
CAGGCCTGGC TCTCGGTGGA TGGAAAGTTC AATATTACGG GTCGATCGCC CGGAAGTGGC 
AGTCGGATGG ATGTGTTGCC TATTCTGTAT CTCGGCGGTC ACGAGATAGC CAATTTCAAT 
ACGCTGCCAC ACGATTTGCC ACTGCATTCG GGATTCCAGG GATGCATATA CGATGTACAG 
CTGAAGGCGG GACAGGTCAC GGTGCCCCTC CAGGAGACGC GCGGGGTCAG AGGTCGGGGC 
GTGGGTCAAT GTGGAACCAG GGAATGCCAT CGCCATGCCT GCCAGCACGA TGGCGCCTGC 
CTGCAGCATG GAGCAACCTT TACTTGCATC TGCCAGGAGG GCTGGTATGG GCCACTGTGT 
GCCCAGCCCA CGAATCCCTG TGACTCCTTC AACAACAAAT GCTACGAGGA CGCCACCTGT 
GTGCCGCTGG TCAATGGCTA CGAATGCGAT TGTCCTGTAG GACGCACAGG CAAAAACTGC 
GAGGAGGTAA TTCGATCCCT GAGCGACGTA TCCCTGACTG GCAGACGATC CTATCTGGCG 
GTTCGCTGGC CATATCTATA CGATGGCGGG GATAAACTGG GCGCTAAGCG CTCCCAAATG 
GTCAGCTACC GGAACTTCAC CAAGAAACTA ATGCCCCCGA AGCCCATTAC CACGCCCAGC 
AGCCACTTTG TGATGAAACT GCTGAATGAG GTGGAGAAGC AGCGCAGCTT CTCGCCAGTT 
CCCCTGATGG GATCGAAGTC CTTCGAGGAG CACCATCGAG TGCAGTTCTT CTTCATCGAG 
TTCCAACTGC GCCCGCTCTC GGAAAGAGGC CTCCTTCTGT ACTTCGGTAC CCTGAACAAC 
AATCAGGACA AGAAGATAGG ATTCGTGTCG CTATCGCTGC AGGGCGGAGT GGTGGAGTTC 
CGGATTTCCG GGCCCAGCAA TCATGTCACG GTGGTGCGGA GCGTTCGCAT GTTGGCCATC 
GGCGAGTGGC ACAAGATCAA GATGGCGCAG CGCGGAAGAT GGCTGACCCT GTGGGTGGAG 
GGTAGCGCCT CGTCGGCTTT GGCTCCTTCG GCTGAGGTGC TCGTCGAGCC CGACTCACTG 
CTCTACATTG GCGGGCTGAA GGATGTGTCC AAGCTGCCGC ACAACGCCAT CTCCGGTTTC 
CCGATTCCGT TCAGGGGATG TGTCCGGGGT CTGGTGGTCA GTGGAACTCG CATTGTGCTC 
AACGAAACCA ACATTGTGGA ATCGCGCAAC ATTCGAGACT GTGATGGCAC CGCTTGTGGC 
GGAGACTCCT GCGAGTCCGG AGGACATTGC TGGCTGGACG AGAAGCTGCA GCCGCATTGC 
ATCTGTCCGG AGTACGCGAA AGGAGATCGA TGCGAGTACT CGGAGACCTG CAAGCTCATT 
CCGTGCAAAA ACAATGGAAG ATGCCTGAGA AGTGGACGCT GCTCGTGTCC AAATGGATGG 
GGTGGCTTCT ACTGCGAGAT TGCCATGAGC AAGCCGACGA CGCCGAGCTT CCGTGGCAAC 
AGTTACCTGA TCTTGCCGCC GCCGCGAATT CCGATGAAAG ACAAGCGGCG GGGACCCTCC 
CTGTACGTCC GCCCCCGCGA AGCCATCCAA GTCTCGCTGA ACTTCTCCAC CATCGAACCG 
GACGGACTGC TCCTGTGGAG CGAGCACGAG AGGAGCAAGT TCCTCGGCCT GGGCCTGGAG 
GCGGGCCACC TCAAGCTGGC CAGCAACCTC CTGGGCAGCA CCAACGACAC GGTCCGTGCT 
CCGGCCAGCG GATTCATAGC CGACGGAGCC TGGCACTGGA CCAGCGTCCT GCTGGACCGG 
TCCAGGCTGG AGCTGCAGCT GGACGGCGAG GTGATCTTCA CGGAGCGATT GCCCGAGGGC 
GGACGGTCAC TGGGCTCGAC CACGCCGAGA AGCACTCTGG CGGGCAGGCG CAAGAATTCG 
AGCAAGGAGC CAACAATTAG CTACGAGGAT GTCTTCTACT TGGGCGGCTT TCCCAACTCG 
GACTCGGTGA GCAGGCGCAC AAAGGGTCGC TTCTTCGATC CCTTCAAGGG CTGCCTGCAG 
GATATCCAGT TCGGAGCCGA GCCCACAGCG ATTATCAGCG ACTTCTCGAC GTACCAGGGC 
GAGAACATTG GATCCTGTGA TCTGCACGGC GATGAGCCGC TTACTGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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eys cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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