Tmtc3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tmtc3
Entrez Gene ID	37401
Full Name	CG4050 gene product from transcript CG4050-RA
Synonyms	Dmel\CG4050,anon-DM192,anon-j
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 57C2-57C2

mRNA and Protein(s)

mRNA	Protein	Name
NM_166426.2	NP_726030.1	transmembrane and tetratricopeptide repeat containing 3, isoform B
NM_057898.4	NP_477246.2	transmembrane and tetratricopeptide repeat containing 3, isoform A

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	TMTC3	XP_532644.1
Danio rerio (zebrafish)	tmtc3	XP_001922486.4
Homo sapiens (human)	TMTC3	NP_861448.2
Bos taurus (cattle)	TMTC3	XP_002687234.2
Rattus norvegicus (Norway rat)	Tmtc3	NP_001129330.1
Drosophila melanogaster (fruit fly)	CG4050	NP_726030.1
Pan troglodytes (chimpanzee)	TMTC3	XP_522486.2
Mus musculus (house mouse)	Tmtc3	NP_001103483.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010599	XP_314563.4
Gallus gallus (chicken)	TMTC3	XP_004937657.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tmtc3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tmtc3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10753	NM_057898.4 Latest version!	Drosophila melanogaster transmembrane and tetratricopeptide repeat containing 3, transcript variant A (Tmtc3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa10753	NM_166426.2 Latest version!	Drosophila melanogaster transmembrane and tetratricopeptide repeat containing 3, transcript variant B (Tmtc3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tmtc3 ( NM_166426.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726030.1 Drosophila melanogaster transmembrane and tetratricopeptide repeat containing 3, transcript variant B (Tmtc3), mRNA.

Price & Availability↑

CloneID	OFa10753
Clone ID Related Accession (Same CDS sequence)	NM_166426.2 , NM_057898.4
Accession Version	NM_166426.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	transmembrane and tetratricopeptide repeat containing 3, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166426.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCAACGA ACCCCAACCC CGGAATCCAT CAGTATGCAC CCTCCACTTT GCCGAGGGAA 
CGGGAGCGGG AAGGAGCCAC AAACTCGCCG CAGCGCAACC TGCTCGAGTT CCTGTGTATA 
TGCGTAGCCT GCATAGTCTG CTATTACAAC AGTACTCAGT GCGGGCTTGT TTTCGATGAC 
ATCAGCGCAA TCAGGGACAA CAAGGATCTG AGGCCGCACA CTCCGCTGAT CAACGTTTTC 
CTGAACGACT TCTGGGGGAC GCCGATGCGC AAGGAGCAGT CCCACAAGTC TTATCGCCCA 
CTCACCGTGC TGACGTTCCG CTTCAACTAC TTGCTGCATG CACTTGAGCC GTTTGGATAC 
CACCTCGTCA ACCTGCTGCT GCACTTGTCG GTCTGCCTTT TGTGGCGTCG GGTCTGCCGG 
TTGCTGCTGC GTCAGTGTGC CGCATCGGGC AGTAATGCCA TCTCGGCACC ATCCTCGTCA 
TCCGTCTCCC AGCTCAACAC ATGCGCTTTC GTGGCCTCGC TGCTCTTCGC CGTGCATCCA 
GTTCACACAG AGGCCGTTAC TGGCGTCGTC GGGCGTGCTG AATTGCTCTC TTCAATTTGC 
TTCCTGGCCG CCTTTCTTAG CTACGCGAAG TCCGTCGGCG ACTCGGGTTG TCCGCGACGC 
ACCAATTGGC TGACGCTTTT TGGCTGCTTT GGCAGTTGCC TGTTGGCCTC CATGCTGTGC 
AAGGAGCAAG GCATCACCAT TGCCGGCATA TGCGTGGTCT ATGAGTTGTT CGTAGTCCAT 
CAACTACGGC CACTGCACCT TTGCCATTTT GTGCTGCGCC TGTTTGACGA GCGGACCGAG 
CAGCAGTCGC CAAAGCTGGC GAATCCTTCG GGAATTCGAC GCTGGTCATC GTCGACGCTG 
TGGAAACGTT TGAGCTTCCT GGTTGGCATC ACGTTGACCC TCCTGGTGGG ACGTGTATAC 
GTGATGGGTT CACAGTTACC CATCTTTACG CGGTTTGATA ATCCAGCCTC TGCTGCGGAT 
ACTCCTGAGA GACAACTTAC CTACGGCTAC CTCATTTACC TGAACTGCTG GCTGCTGTTA 
TGCCCATCGC TGCTCTGCTG CGACTGGACA ATGGGCACTG TTCCGTTGCT GCAGGGATTC 
ACGGATTCCC GCAACATAAC CACTTTGCTT ACCTTTCTGG CATTGGGGGC TATGGTCGCC 
AAGACCTGCT TTACCCGCAA TCTGGCCCTG TCGCGGACAC TTATAATGTG CCTGGGCTGG 
ATGGTGCTGC CCTTTCTCCC AGCCTCTAAT CTGTTCTTCC CCGTTGGATT TGTGGTGGCA 
GAACGCATTT TGTACATGCC ATCCATGGGG TACTGCTTGC TGGTGGCTTA TGGCTTTGAG 
CAGCTTCAGC GCCGTGGAAG CCTAAGCTGG CAACGTTTTT CACAAGCTGC TCTCGCTATC 
CTGCTCCTCA CGCACGCGCT GAAAACGCAT CAACGCAATT TAGATTGGCG TACAGAATAC 
TCCCTGTTCA TGTCTGGTGT GCATGTAAAT CAGCGCAACG CCAAGCTGTA CAATAACGTG 
GGACACGCTT TGGAGAACGA AGGGAAGTTC GAAGAGGCAT TGCTCTATTT CCAGCAGGCC 
GTGCGCATTC AGACCGATGA CATCGGGGCA CACATCAATG TGGGTCGCAC GTTCAATAAT 
CTCAAGCGGT ATGCAGAGGC GGAACAAGCC TATGTTCAGG CTAAGGCTCT GTTTCCACAA 
GCTAAGCCGG GTGTCAGCTA TCATGCGCGC ATCGCTCCCA ATCACTTGAA TGTGTTTATC 
AACCTGGCGA ATCTCATAGC TAAAAATCAA ACGCGTCTGG AGGAGGCTGA TCATCTCTAT 
CGCCAGGCTA TCAGCATGAG GAGTGACTAT GTTCAAGCTT ACATCAATCG TGGCGACATT 
CTTATGAAGT TGAATCGCAC AGCTCAGGCG CAGGAGGTCT ATGAGCAGGC ACTGCTTTAT 
GATAATGAAA ATGCAGACAT CTACTACAAT CTGGGCGTAG TTTTCCTAGA GCAGGGCAAG 
AGCCAGCAGG CGCAGGTGTA TTTTAATAAG GCAATCGAAT TGTATCCGGA GCACGAACAG 
GCATTGCTGA ACTCGGCTAT TCTGCTGCAG GAACTCGGTG GCGAGGAGGC CCGCCGGGTG 
TCACGCTCTC GGCTGTACAA AGTTCTGGAG AACGATGATC AGAACGAAAA GGTGTACTTC 
AACCTGGGTA TGCTGGCCAT GGACGAGTCA AGCTTCGACG AGGCTGAGCA GTTTTTCAAG 
AGAGCTATAC ACCTAAAGGC AGACTTTCGT AGTGCACTTT TTAATCTGGC TCTGCTACTG 
GCCGATACAA AACGGCCCTT GGATGCAGTG CCATTTCTGA ATCAACTGAT TCGACATCAT 
CCGTCGCATG TTAAAGGACT GATCCTGCTG GGCGACATCT ACATTAATCA CATGAAGGAT 
CTGGATGAGG CGGAGAAGTG CTATCGCAGC ATACTTCACT ACGATCCCCA CAACACTCAG 
GGCTTGCACA ATCTCTGTGT GGTGTTCGTG GAACGTAAGC GGCTGGCCAA GGCAGCTGCA 
TGCCTGCAGT ACGCGCAACG CTTGGCACCC GCCGAGGACT ATATCGGTCG GCATTTGCAG 
ATTGTCCTTG CACGACTGCA GAAAATCAAC AAGTTACCTG AGTCGGCGCC AGAGCGCAAG 
CTCGCGTATG AGGACTACGA TCCGCTTGAG TTTAAACTGC CCCAGGATCG ACCAACGCAT 
AAATCGCGTA AACGATCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726030.1
CDS	300..3080
Translation	MSTNPNPGIHQYAPSTLPREREREGATNSPQRNLLEFLCICVACIVCYYNSTQCGLVFDDISAIRDNKDLRPHTPLINVFLNDFWGTPMRKEQSHKSYRPLTVLTFRFNYLLHALEPFGYHLVNLLLHLSVCLLWRRVCRLLLRQCAASGSNAISAPSSSSVSQLNTCAFVASLLFAVHPVHTEAVTGVVGRAELLSSICFLAAFLSYAKSVGDSGCPRRTNWLTLFGCFGSCLLASMLCKEQGITIAGICVVYELFVVHQLRPLHLCHFVLRLFDERTEQQSPKLANPSGIRRWSSSTLWKRLSFLVGITLTLLVGRVYVMGSQLPIFTRFDNPASAADTPERQLTYGYLIYLNCWLLLCPSLLCCDWTMGTVPLLQGFTDSRNITTLLTFLALGAMVAKTCFTRNLALSRTLIMCLGWMVLPFLPASNLFFPVGFVVAERILYMPSMGYCLLVAYGFEQLQRRGSLSWQRFSQAALAILLLTHALKTHQRNLDWRTEYSLFMSGVHVNQRNAKLYNNVGHALENEGKFEEALLYFQQAVRIQTDDIGAHINVGRTFNNLKRYAEAEQAYVQAKALFPQAKPGVSYHARIAPNHLNVFINLANLIAKNQTRLEEADHLYRQAISMRSDYVQAYINRGDILMKLNRTAQAQEVYEQALLYDNENADIYYNLGVVFLEQGKSQQAQVYFNKAIELYPEHEQALLNSAILLQELGGEEARRVSRSRLYKVLENDDQNEKVYFNLGMLAMDESSFDEAEQFFKRAIHLKADFRSALFNLALLLADTKRPLDAVPFLNQLIRHHPSHVKGLILLGDIYINHMKDLDEAEKCYRSILHYDPHNTQGLHNLCVVFVERKRLAKAAACLQYAQRLAPAEDYIGRHLQIVLARLQKINKLPESAPERKLAYEDYDPLEFKLPQDRPTHKSRKRS

Target ORF information:

RefSeq Version	NM_166426.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster transmembrane and tetratricopeptide repeat containing 3, transcript variant B (Tmtc3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166426.2

ORF Insert Sequence:

ATGTCAACGA ACCCCAACCC CGGAATCCAT CAGTATGCAC CCTCCACTTT GCCGAGGGAA 
CGGGAGCGGG AAGGAGCCAC AAACTCGCCG CAGCGCAACC TGCTCGAGTT CCTGTGTATA 
TGCGTAGCCT GCATAGTCTG CTATTACAAC AGTACTCAGT GCGGGCTTGT TTTCGATGAC 
ATCAGCGCAA TCAGGGACAA CAAGGATCTG AGGCCGCACA CTCCGCTGAT CAACGTTTTC 
CTGAACGACT TCTGGGGGAC GCCGATGCGC AAGGAGCAGT CCCACAAGTC TTATCGCCCA 
CTCACCGTGC TGACGTTCCG CTTCAACTAC TTGCTGCATG CACTTGAGCC GTTTGGATAC 
CACCTCGTCA ACCTGCTGCT GCACTTGTCG GTCTGCCTTT TGTGGCGTCG GGTCTGCCGG 
TTGCTGCTGC GTCAGTGTGC CGCATCGGGC AGTAATGCCA TCTCGGCACC ATCCTCGTCA 
TCCGTCTCCC AGCTCAACAC ATGCGCTTTC GTGGCCTCGC TGCTCTTCGC CGTGCATCCA 
GTTCACACAG AGGCCGTTAC TGGCGTCGTC GGGCGTGCTG AATTGCTCTC TTCAATTTGC 
TTCCTGGCCG CCTTTCTTAG CTACGCGAAG TCCGTCGGCG ACTCGGGTTG TCCGCGACGC 
ACCAATTGGC TGACGCTTTT TGGCTGCTTT GGCAGTTGCC TGTTGGCCTC CATGCTGTGC 
AAGGAGCAAG GCATCACCAT TGCCGGCATA TGCGTGGTCT ATGAGTTGTT CGTAGTCCAT 
CAACTACGGC CACTGCACCT TTGCCATTTT GTGCTGCGCC TGTTTGACGA GCGGACCGAG 
CAGCAGTCGC CAAAGCTGGC GAATCCTTCG GGAATTCGAC GCTGGTCATC GTCGACGCTG 
TGGAAACGTT TGAGCTTCCT GGTTGGCATC ACGTTGACCC TCCTGGTGGG ACGTGTATAC 
GTGATGGGTT CACAGTTACC CATCTTTACG CGGTTTGATA ATCCAGCCTC TGCTGCGGAT 
ACTCCTGAGA GACAACTTAC CTACGGCTAC CTCATTTACC TGAACTGCTG GCTGCTGTTA 
TGCCCATCGC TGCTCTGCTG CGACTGGACA ATGGGCACTG TTCCGTTGCT GCAGGGATTC 
ACGGATTCCC GCAACATAAC CACTTTGCTT ACCTTTCTGG CATTGGGGGC TATGGTCGCC 
AAGACCTGCT TTACCCGCAA TCTGGCCCTG TCGCGGACAC TTATAATGTG CCTGGGCTGG 
ATGGTGCTGC CCTTTCTCCC AGCCTCTAAT CTGTTCTTCC CCGTTGGATT TGTGGTGGCA 
GAACGCATTT TGTACATGCC ATCCATGGGG TACTGCTTGC TGGTGGCTTA TGGCTTTGAG 
CAGCTTCAGC GCCGTGGAAG CCTAAGCTGG CAACGTTTTT CACAAGCTGC TCTCGCTATC 
CTGCTCCTCA CGCACGCGCT GAAAACGCAT CAACGCAATT TAGATTGGCG TACAGAATAC 
TCCCTGTTCA TGTCTGGTGT GCATGTAAAT CAGCGCAACG CCAAGCTGTA CAATAACGTG 
GGACACGCTT TGGAGAACGA AGGGAAGTTC GAAGAGGCAT TGCTCTATTT CCAGCAGGCC 
GTGCGCATTC AGACCGATGA CATCGGGGCA CACATCAATG TGGGTCGCAC GTTCAATAAT 
CTCAAGCGGT ATGCAGAGGC GGAACAAGCC TATGTTCAGG CTAAGGCTCT GTTTCCACAA 
GCTAAGCCGG GTGTCAGCTA TCATGCGCGC ATCGCTCCCA ATCACTTGAA TGTGTTTATC 
AACCTGGCGA ATCTCATAGC TAAAAATCAA ACGCGTCTGG AGGAGGCTGA TCATCTCTAT 
CGCCAGGCTA TCAGCATGAG GAGTGACTAT GTTCAAGCTT ACATCAATCG TGGCGACATT 
CTTATGAAGT TGAATCGCAC AGCTCAGGCG CAGGAGGTCT ATGAGCAGGC ACTGCTTTAT 
GATAATGAAA ATGCAGACAT CTACTACAAT CTGGGCGTAG TTTTCCTAGA GCAGGGCAAG 
AGCCAGCAGG CGCAGGTGTA TTTTAATAAG GCAATCGAAT TGTATCCGGA GCACGAACAG 
GCATTGCTGA ACTCGGCTAT TCTGCTGCAG GAACTCGGTG GCGAGGAGGC CCGCCGGGTG 
TCACGCTCTC GGCTGTACAA AGTTCTGGAG AACGATGATC AGAACGAAAA GGTGTACTTC 
AACCTGGGTA TGCTGGCCAT GGACGAGTCA AGCTTCGACG AGGCTGAGCA GTTTTTCAAG 
AGAGCTATAC ACCTAAAGGC AGACTTTCGT AGTGCACTTT TTAATCTGGC TCTGCTACTG 
GCCGATACAA AACGGCCCTT GGATGCAGTG CCATTTCTGA ATCAACTGAT TCGACATCAT 
CCGTCGCATG TTAAAGGACT GATCCTGCTG GGCGACATCT ACATTAATCA CATGAAGGAT 
CTGGATGAGG CGGAGAAGTG CTATCGCAGC ATACTTCACT ACGATCCCCA CAACACTCAG 
GGCTTGCACA ATCTCTGTGT GGTGTTCGTG GAACGTAAGC GGCTGGCCAA GGCAGCTGCA 
TGCCTGCAGT ACGCGCAACG CTTGGCACCC GCCGAGGACT ATATCGGTCG GCATTTGCAG 
ATTGTCCTTG CACGACTGCA GAAAATCAAC AAGTTACCTG AGTCGGCGCC AGAGCGCAAG 
CTCGCGTATG AGGACTACGA TCCGCTTGAG TTTAAACTGC CCCAGGATCG ACCAACGCAT 
AAATCGCGTA AACGATCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tmtc3 ( NM_057898.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477246.2 Drosophila melanogaster transmembrane and tetratricopeptide repeat containing 3, transcript variant A (Tmtc3), mRNA.

Price & Availability↑

CloneID	OFa10753
Clone ID Related Accession (Same CDS sequence)	NM_166426.2 , NM_057898.4
Accession Version	NM_057898.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	transmembrane and tetratricopeptide repeat containing 3, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_057898.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCAACGA ACCCCAACCC CGGAATCCAT CAGTATGCAC CCTCCACTTT GCCGAGGGAA 
CGGGAGCGGG AAGGAGCCAC AAACTCGCCG CAGCGCAACC TGCTCGAGTT CCTGTGTATA 
TGCGTAGCCT GCATAGTCTG CTATTACAAC AGTACTCAGT GCGGGCTTGT TTTCGATGAC 
ATCAGCGCAA TCAGGGACAA CAAGGATCTG AGGCCGCACA CTCCGCTGAT CAACGTTTTC 
CTGAACGACT TCTGGGGGAC GCCGATGCGC AAGGAGCAGT CCCACAAGTC TTATCGCCCA 
CTCACCGTGC TGACGTTCCG CTTCAACTAC TTGCTGCATG CACTTGAGCC GTTTGGATAC 
CACCTCGTCA ACCTGCTGCT GCACTTGTCG GTCTGCCTTT TGTGGCGTCG GGTCTGCCGG 
TTGCTGCTGC GTCAGTGTGC CGCATCGGGC AGTAATGCCA TCTCGGCACC ATCCTCGTCA 
TCCGTCTCCC AGCTCAACAC ATGCGCTTTC GTGGCCTCGC TGCTCTTCGC CGTGCATCCA 
GTTCACACAG AGGCCGTTAC TGGCGTCGTC GGGCGTGCTG AATTGCTCTC TTCAATTTGC 
TTCCTGGCCG CCTTTCTTAG CTACGCGAAG TCCGTCGGCG ACTCGGGTTG TCCGCGACGC 
ACCAATTGGC TGACGCTTTT TGGCTGCTTT GGCAGTTGCC TGTTGGCCTC CATGCTGTGC 
AAGGAGCAAG GCATCACCAT TGCCGGCATA TGCGTGGTCT ATGAGTTGTT CGTAGTCCAT 
CAACTACGGC CACTGCACCT TTGCCATTTT GTGCTGCGCC TGTTTGACGA GCGGACCGAG 
CAGCAGTCGC CAAAGCTGGC GAATCCTTCG GGAATTCGAC GCTGGTCATC GTCGACGCTG 
TGGAAACGTT TGAGCTTCCT GGTTGGCATC ACGTTGACCC TCCTGGTGGG ACGTGTATAC 
GTGATGGGTT CACAGTTACC CATCTTTACG CGGTTTGATA ATCCAGCCTC TGCTGCGGAT 
ACTCCTGAGA GACAACTTAC CTACGGCTAC CTCATTTACC TGAACTGCTG GCTGCTGTTA 
TGCCCATCGC TGCTCTGCTG CGACTGGACA ATGGGCACTG TTCCGTTGCT GCAGGGATTC 
ACGGATTCCC GCAACATAAC CACTTTGCTT ACCTTTCTGG CATTGGGGGC TATGGTCGCC 
AAGACCTGCT TTACCCGCAA TCTGGCCCTG TCGCGGACAC TTATAATGTG CCTGGGCTGG 
ATGGTGCTGC CCTTTCTCCC AGCCTCTAAT CTGTTCTTCC CCGTTGGATT TGTGGTGGCA 
GAACGCATTT TGTACATGCC ATCCATGGGG TACTGCTTGC TGGTGGCTTA TGGCTTTGAG 
CAGCTTCAGC GCCGTGGAAG CCTAAGCTGG CAACGTTTTT CACAAGCTGC TCTCGCTATC 
CTGCTCCTCA CGCACGCGCT GAAAACGCAT CAACGCAATT TAGATTGGCG TACAGAATAC 
TCCCTGTTCA TGTCTGGTGT GCATGTAAAT CAGCGCAACG CCAAGCTGTA CAATAACGTG 
GGACACGCTT TGGAGAACGA AGGGAAGTTC GAAGAGGCAT TGCTCTATTT CCAGCAGGCC 
GTGCGCATTC AGACCGATGA CATCGGGGCA CACATCAATG TGGGTCGCAC GTTCAATAAT 
CTCAAGCGGT ATGCAGAGGC GGAACAAGCC TATGTTCAGG CTAAGGCTCT GTTTCCACAA 
GCTAAGCCGG GTGTCAGCTA TCATGCGCGC ATCGCTCCCA ATCACTTGAA TGTGTTTATC 
AACCTGGCGA ATCTCATAGC TAAAAATCAA ACGCGTCTGG AGGAGGCTGA TCATCTCTAT 
CGCCAGGCTA TCAGCATGAG GAGTGACTAT GTTCAAGCTT ACATCAATCG TGGCGACATT 
CTTATGAAGT TGAATCGCAC AGCTCAGGCG CAGGAGGTCT ATGAGCAGGC ACTGCTTTAT 
GATAATGAAA ATGCAGACAT CTACTACAAT CTGGGCGTAG TTTTCCTAGA GCAGGGCAAG 
AGCCAGCAGG CGCAGGTGTA TTTTAATAAG GCAATCGAAT TGTATCCGGA GCACGAACAG 
GCATTGCTGA ACTCGGCTAT TCTGCTGCAG GAACTCGGTG GCGAGGAGGC CCGCCGGGTG 
TCACGCTCTC GGCTGTACAA AGTTCTGGAG AACGATGATC AGAACGAAAA GGTGTACTTC 
AACCTGGGTA TGCTGGCCAT GGACGAGTCA AGCTTCGACG AGGCTGAGCA GTTTTTCAAG 
AGAGCTATAC ACCTAAAGGC AGACTTTCGT AGTGCACTTT TTAATCTGGC TCTGCTACTG 
GCCGATACAA AACGGCCCTT GGATGCAGTG CCATTTCTGA ATCAACTGAT TCGACATCAT 
CCGTCGCATG TTAAAGGACT GATCCTGCTG GGCGACATCT ACATTAATCA CATGAAGGAT 
CTGGATGAGG CGGAGAAGTG CTATCGCAGC ATACTTCACT ACGATCCCCA CAACACTCAG 
GGCTTGCACA ATCTCTGTGT GGTGTTCGTG GAACGTAAGC GGCTGGCCAA GGCAGCTGCA 
TGCCTGCAGT ACGCGCAACG CTTGGCACCC GCCGAGGACT ATATCGGTCG GCATTTGCAG 
ATTGTCCTTG CACGACTGCA GAAAATCAAC AAGTTACCTG AGTCGGCGCC AGAGCGCAAG 
CTCGCGTATG AGGACTACGA TCCGCTTGAG TTTAAACTGC CCCAGGATCG ACCAACGCAT 
AAATCGCGTA AACGATCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477246.2
CDS	224..3004
Translation	MSTNPNPGIHQYAPSTLPREREREGATNSPQRNLLEFLCICVACIVCYYNSTQCGLVFDDISAIRDNKDLRPHTPLINVFLNDFWGTPMRKEQSHKSYRPLTVLTFRFNYLLHALEPFGYHLVNLLLHLSVCLLWRRVCRLLLRQCAASGSNAISAPSSSSVSQLNTCAFVASLLFAVHPVHTEAVTGVVGRAELLSSICFLAAFLSYAKSVGDSGCPRRTNWLTLFGCFGSCLLASMLCKEQGITIAGICVVYELFVVHQLRPLHLCHFVLRLFDERTEQQSPKLANPSGIRRWSSSTLWKRLSFLVGITLTLLVGRVYVMGSQLPIFTRFDNPASAADTPERQLTYGYLIYLNCWLLLCPSLLCCDWTMGTVPLLQGFTDSRNITTLLTFLALGAMVAKTCFTRNLALSRTLIMCLGWMVLPFLPASNLFFPVGFVVAERILYMPSMGYCLLVAYGFEQLQRRGSLSWQRFSQAALAILLLTHALKTHQRNLDWRTEYSLFMSGVHVNQRNAKLYNNVGHALENEGKFEEALLYFQQAVRIQTDDIGAHINVGRTFNNLKRYAEAEQAYVQAKALFPQAKPGVSYHARIAPNHLNVFINLANLIAKNQTRLEEADHLYRQAISMRSDYVQAYINRGDILMKLNRTAQAQEVYEQALLYDNENADIYYNLGVVFLEQGKSQQAQVYFNKAIELYPEHEQALLNSAILLQELGGEEARRVSRSRLYKVLENDDQNEKVYFNLGMLAMDESSFDEAEQFFKRAIHLKADFRSALFNLALLLADTKRPLDAVPFLNQLIRHHPSHVKGLILLGDIYINHMKDLDEAEKCYRSILHYDPHNTQGLHNLCVVFVERKRLAKAAACLQYAQRLAPAEDYIGRHLQIVLARLQKINKLPESAPERKLAYEDYDPLEFKLPQDRPTHKSRKRS

Target ORF information:

RefSeq Version	NM_057898.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster transmembrane and tetratricopeptide repeat containing 3, transcript variant A (Tmtc3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057898.4

ORF Insert Sequence:

ATGTCAACGA ACCCCAACCC CGGAATCCAT CAGTATGCAC CCTCCACTTT GCCGAGGGAA 
CGGGAGCGGG AAGGAGCCAC AAACTCGCCG CAGCGCAACC TGCTCGAGTT CCTGTGTATA 
TGCGTAGCCT GCATAGTCTG CTATTACAAC AGTACTCAGT GCGGGCTTGT TTTCGATGAC 
ATCAGCGCAA TCAGGGACAA CAAGGATCTG AGGCCGCACA CTCCGCTGAT CAACGTTTTC 
CTGAACGACT TCTGGGGGAC GCCGATGCGC AAGGAGCAGT CCCACAAGTC TTATCGCCCA 
CTCACCGTGC TGACGTTCCG CTTCAACTAC TTGCTGCATG CACTTGAGCC GTTTGGATAC 
CACCTCGTCA ACCTGCTGCT GCACTTGTCG GTCTGCCTTT TGTGGCGTCG GGTCTGCCGG 
TTGCTGCTGC GTCAGTGTGC CGCATCGGGC AGTAATGCCA TCTCGGCACC ATCCTCGTCA 
TCCGTCTCCC AGCTCAACAC ATGCGCTTTC GTGGCCTCGC TGCTCTTCGC CGTGCATCCA 
GTTCACACAG AGGCCGTTAC TGGCGTCGTC GGGCGTGCTG AATTGCTCTC TTCAATTTGC 
TTCCTGGCCG CCTTTCTTAG CTACGCGAAG TCCGTCGGCG ACTCGGGTTG TCCGCGACGC 
ACCAATTGGC TGACGCTTTT TGGCTGCTTT GGCAGTTGCC TGTTGGCCTC CATGCTGTGC 
AAGGAGCAAG GCATCACCAT TGCCGGCATA TGCGTGGTCT ATGAGTTGTT CGTAGTCCAT 
CAACTACGGC CACTGCACCT TTGCCATTTT GTGCTGCGCC TGTTTGACGA GCGGACCGAG 
CAGCAGTCGC CAAAGCTGGC GAATCCTTCG GGAATTCGAC GCTGGTCATC GTCGACGCTG 
TGGAAACGTT TGAGCTTCCT GGTTGGCATC ACGTTGACCC TCCTGGTGGG ACGTGTATAC 
GTGATGGGTT CACAGTTACC CATCTTTACG CGGTTTGATA ATCCAGCCTC TGCTGCGGAT 
ACTCCTGAGA GACAACTTAC CTACGGCTAC CTCATTTACC TGAACTGCTG GCTGCTGTTA 
TGCCCATCGC TGCTCTGCTG CGACTGGACA ATGGGCACTG TTCCGTTGCT GCAGGGATTC 
ACGGATTCCC GCAACATAAC CACTTTGCTT ACCTTTCTGG CATTGGGGGC TATGGTCGCC 
AAGACCTGCT TTACCCGCAA TCTGGCCCTG TCGCGGACAC TTATAATGTG CCTGGGCTGG 
ATGGTGCTGC CCTTTCTCCC AGCCTCTAAT CTGTTCTTCC CCGTTGGATT TGTGGTGGCA 
GAACGCATTT TGTACATGCC ATCCATGGGG TACTGCTTGC TGGTGGCTTA TGGCTTTGAG 
CAGCTTCAGC GCCGTGGAAG CCTAAGCTGG CAACGTTTTT CACAAGCTGC TCTCGCTATC 
CTGCTCCTCA CGCACGCGCT GAAAACGCAT CAACGCAATT TAGATTGGCG TACAGAATAC 
TCCCTGTTCA TGTCTGGTGT GCATGTAAAT CAGCGCAACG CCAAGCTGTA CAATAACGTG 
GGACACGCTT TGGAGAACGA AGGGAAGTTC GAAGAGGCAT TGCTCTATTT CCAGCAGGCC 
GTGCGCATTC AGACCGATGA CATCGGGGCA CACATCAATG TGGGTCGCAC GTTCAATAAT 
CTCAAGCGGT ATGCAGAGGC GGAACAAGCC TATGTTCAGG CTAAGGCTCT GTTTCCACAA 
GCTAAGCCGG GTGTCAGCTA TCATGCGCGC ATCGCTCCCA ATCACTTGAA TGTGTTTATC 
AACCTGGCGA ATCTCATAGC TAAAAATCAA ACGCGTCTGG AGGAGGCTGA TCATCTCTAT 
CGCCAGGCTA TCAGCATGAG GAGTGACTAT GTTCAAGCTT ACATCAATCG TGGCGACATT 
CTTATGAAGT TGAATCGCAC AGCTCAGGCG CAGGAGGTCT ATGAGCAGGC ACTGCTTTAT 
GATAATGAAA ATGCAGACAT CTACTACAAT CTGGGCGTAG TTTTCCTAGA GCAGGGCAAG 
AGCCAGCAGG CGCAGGTGTA TTTTAATAAG GCAATCGAAT TGTATCCGGA GCACGAACAG 
GCATTGCTGA ACTCGGCTAT TCTGCTGCAG GAACTCGGTG GCGAGGAGGC CCGCCGGGTG 
TCACGCTCTC GGCTGTACAA AGTTCTGGAG AACGATGATC AGAACGAAAA GGTGTACTTC 
AACCTGGGTA TGCTGGCCAT GGACGAGTCA AGCTTCGACG AGGCTGAGCA GTTTTTCAAG 
AGAGCTATAC ACCTAAAGGC AGACTTTCGT AGTGCACTTT TTAATCTGGC TCTGCTACTG 
GCCGATACAA AACGGCCCTT GGATGCAGTG CCATTTCTGA ATCAACTGAT TCGACATCAT 
CCGTCGCATG TTAAAGGACT GATCCTGCTG GGCGACATCT ACATTAATCA CATGAAGGAT 
CTGGATGAGG CGGAGAAGTG CTATCGCAGC ATACTTCACT ACGATCCCCA CAACACTCAG 
GGCTTGCACA ATCTCTGTGT GGTGTTCGTG GAACGTAAGC GGCTGGCCAA GGCAGCTGCA 
TGCCTGCAGT ACGCGCAACG CTTGGCACCC GCCGAGGACT ATATCGGTCG GCATTTGCAG 
ATTGTCCTTG CACGACTGCA GAAAATCAAC AAGTTACCTG AGTCGGCGCC AGAGCGCAAG 
CTCGCGTATG AGGACTACGA TCCGCTTGAG TTTAAACTGC CCCAGGATCG ACCAACGCAT 
AAATCGCGTA AACGATCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Tmtc3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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