18w cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	18w
Entrez Gene ID	37277
Full Name	18 wheeler
Synonyms	18-w,18-wheeler,18W,CG8896,CT25100,Dmel\CG8896,Toll-2,l(2)00053,tlr,toll
General protein information	Preferred Names 18 wheeler Names CG8896 gene product from transcript CG8896-RA 18-wheeler 18Wheeler 18w-PA CG8896-PA Toll-2 toll like receptor toll receptor wheeler
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 56F8-56F8

mRNA and Protein(s)

mRNA	Protein	Name
NM_057466.2	NP_476814.1	18 wheeler

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Median mRNA expression of TLR2 and TLR4 of mononuclear cells was not altered, and there was no obvious trend over time.
Title: Effect of major abdominal surgery on endotoxin release and expression of Toll-like receptors 2/4.

18W protein plays an adhesive or signaling role in regions of the epithelium engaged in cell migration.
Title: Expression of 18-wheeler in the follicle cell epithelium affects cell migration and egg morphology in Drosophila.

Data show that in larvae, 18wheeler is required for normal fat body development, and in mutant larvae induction of all antimicrobial peptide genes is compromised.
Title: Critical evaluation of the role of the Toll-like receptor 18-Wheeler in the host defense of Drosophila.

The following 18w gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the 18w cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13104	NM_057466.2 Latest version!	Drosophila melanogaster 18 wheeler (18w), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

18w ( NM_057466.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476814.1 Drosophila melanogaster 18 wheeler (18w), mRNA.

Price & Availability↑

CloneID	OFa13104
Clone ID Related Accession (Same CDS sequence)	NM_057466.2
Accession Version	NM_057466.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	18 wheeler
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Mar 19, 2002 this sequence version replaced NM_057466.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCAGCCA CATCTTCCAT CATCACCATC ATCGCAGTCG CTGCCTGCCT GTTGCTTCTT 
GTGGCTGATG CGCATGCGCA GCAGCAGTGC AACTGGCAGT ACGGCCTCAC CACCATGGAC 
ATCCGGTGCA GTGTCCGCGC TCTGGAGTCC GGCACCGGAA CGCCACTTGA CCTCCAGGTG 
GCGGAGGCGG CCGGCCGCCT GGATCTGCAG TGCAGCCAGG AGCTCCTGCA TGCCAGTGAA 
CTGGCACCGG GACTCTTCCG GCAACTGCAG AAGCTCTCGG AGCTGCGAAT CGATGCCTGC 
AAACTGCAGC GAGTGCCTCC AAATGCATTT GAGGGTCTGA TGTCGCTCAA GCGACTCACC 
CTGGAGTCAC ACAACGCGGT CTGGGGTCCG GGAAAGACCC TGGAGTTGCA CGGCCAGTCG 
TTCCAGGGAC TGAAGGAGCT GTCCGAGCTG CACTTGGGCG ACAACAATAT CCGTCAGTTG 
CCCGAGGGCG TCTGGTGCTC CATGCCCAGC CTGCAACTGC TCAATCTCAC CCAGAACCGG 
ATACGTTCCG CCGAGTTCCT AGGCTTCTCC GAGAAACTCT GTGCCGGCTC AGCCCTGAGC 
AATGCCAATG GAGCAGTTAG CGGAGGCTCA GAGCTGCAGA CATTGGATGT CTCGTTCAAC 
GAGCTGCGTT CTTTGCCGGA TGCCTGGGGA GCGTCCCGAT TGAGGCGCCT GCAGACCCTC 
AGCCTGCAAC ACAACAACAT CAGCACCCTG GCTCCCAATG CCCTGGCCGG TCTGAGTTCG 
CTGCGAGTGC TGAATATCTC GTACAACCAC CTCGTTTCCC TGCCCTCAGA AGCCTTTGCC 
GGCAACAAGG AGTTGAGGGA ACTGCATCTG CAGGGCAATG ATCTGTACGA ATTGCCCAAA 
GGTCTGTTGC ACCGTCTGGA GCAGCTGTTA GTTCTCGATC TGAGTGGCAA TCAGCTGACC 
AGCCACCATG TTGACAACAG CACCTTCGCC GGTCTGATCC GCCTGATTGT GCTGAATCTG 
TCGAACAATG CCCTCACCCG CATTGGATCC AAAACCTTCA AGGAGCTGTA CTTCCTGCAG 
ATTCTGGACA TGCGAAACAA CTCCATTGGC CACATTGAGG AGGGCGCATT CCTGCCCCTG 
TACAATCTGC ACACCCTCAA TCTCGCCGAG AATCGCCTGC ACACACTGGA CAACCGCATC 
TTCAACGGAT TGTATGTGCT GACGAAACTC ACGCTAAACA ACAATCTTGT TAGCATTGTG 
GAGTCGCAAG CCTTCCGCAA CTGTTCCGAT CTGAAGGAAC TGGATCTCAG CTCCAATCAG 
CTGACTGAAG TGCCCGAGGC TGTGCAAGAT TTGAGCATGC TCAAGACTCT CGATCTCGGA 
GAGAATCAGA TCTCGGAATT CAAGAACAAC ACCTTCCGCA ATCTCAATCA GCTGACGGGT 
CTGCGATTGA TCGACAATCG CATCGGCAAC ATCACCGTAG GCATGTTTCA GGATCTGCCC 
CGTTTGAGTG TTCTCAACCT GGCCAAGAAC CGCATCCAGA GCATCGAGCG TGGAGCCTTC 
GACAAAAACA CCGAGATCGA GGCGATCCGA TTGGACAAGA ACTTCCTCAC CGACATCAAT 
GGAATCTTTG CCACTCTCGC CTCCTTGCTG TGGCTGAATC TCTCCGAGAA TCACCTTGTT 
TGGTTCGATT ACGCCTTCAT CCCATCGAAC TTGAAATGGC TGGATATCCA TGGAAACTAC 
ATCGAAGCCC TGGGCAACTA CTACAAACTG CAAGAGGAGA TTCGTGTGAC CACGCTGGAT 
GCCTCTCACA ACCGCATCAC GGAGATTGGC GCCATGTCGG TGCCGAATTC CATCGAGCTG 
CTGTTCATCA ACAACAACAT CATTGGACAG ATCCAGGCCA ACACCTTTGT GGACAAGACG 
CGTCTGGCAC GCGTGGATCT GTATGCCAAT GTGCTGTCCA AAATCTCGCT TAATGCTCTA 
CGCGTAGCCC CAGTTTCAGC GGAGAAACCA GTTCCCGAGT TTTACCTGGG CGGCAATCCC 
TTTGAGTGCG ATTGCTCCAT GGAGTGGCTG CAGAGGATTA ACAATCTGAC CACCCGCCAG 
CATCCTCATG TTGTGGATCT GGGAAACATC GAGTGCCTGA TGCCGCACAG CCGCAGTGCT 
CCACTGCGTC CGCTGGCCAG TCTCTCCGCC TCCGACTTTG TGTGCAAGTA TGAGAGCCAC 
TGCCCGCCCA CGTGCCACTG CTGCGAGTAC GAACAGTGCG AGTGTGAAGT GATCTGCCCT 
GGAAACTGCA GCTGCTTCCA CGACGCCACC TGGGCCACCA ACATCGTGGA CTGCGGTCGC 
CAGGATCTGG CCGCCCTGCC CAACCGTATC CCTCAGGATG TGAGCGATCT CTACTTGGAT 
GGCAACAACA TGCCCGAGCT GGAGGTTGGC CACCTAACCG GTCGTCGCAA TCTGCGGGCT 
CTCTACCTGA ACGCCTCCAA TCTGATGACG CTGCAGAACG GAAGTCTGGC CCAGTTGGTC 
AATCTTCGAG TGCTCCACTT GGAGAACAAC AAACTAACCG CCCTGGAAGG CACCGAGTTC 
CGCTCCCTGG GACTTTTGCG GGAGCTGTAT CTGCACAACA ACATGCTGAC CCACATCTCG 
AATGCCACCT TTGAGCCACT GGTGTCCCTG GAGGTGCTGC GTCTGGACAA CAACCGCCTG 
AGTTCCCTGC CCCATTTGCA ATATCGTCAC AGCCTCCAGG GACTGACGCT GGGCAGAAAT 
GCCTGGTCAT GCCGCTGCCA GCAGTTGAGG GAATTGGCGC AGTTTGTCTC CGATAACGCC 
ATGGTGGTGC GGGATGCCCA CGATATCTAC TGCTTGGATG CGGGCATCAA GCGCGAGCTG 
GAACTGATTG GCAACCTGGC CAACGGACCC GATTGTTCCG ACCTTCTGGA TGCCAGTGCC 
AGCAATATCA GCTCCTCGCA GGATTTGGCC GGAGGCTATA GGCTGCCCTT GTTGGCCGCT 
GTGCTAGTGC TGATCTTCCT GGTCGTGGTG CTCATCATTG TCTTCGTCTT CCGCGAGAGC 
GTGCGCATGT GGCTCTTCGC CCACTACGGA GTCAGGGTGT GCGAGCCCCG CTTCGAGGAT 
GCCGGCAAAC TGTACGACGC CATCATCCTG CACTCGGAGA AGGACTACGA GTTTGTGTGC 
CGCAACATCG CCGCCGAACT GGAGCATGGT CGACCACCCT TCCGGCTCTG CATTCAGCAG 
CGAGATCTGC CTCCCCAGGC ATCCCACCTT CAGCTGGTGG AGGGAGCAAG GGCGTCGAGG 
AAGATCATCC TGGTGCTGAC CCGCAATCTC TTGGCCACCG AATGGAATCG CATTGAGTTC 
CGTAATGCTT TCCACGAGTC CCTGAGGGGC TTGGCCCAGA AGCTGGTGAT CATCGAGGAG 
ACAAGTGTTT CCGCCGAGGC CGAGGACGTT GCCGAGTTGT CGCCGTACTT GAAATCGGTA 
CCCTCCAACC GACTGCTGAC CTGCGACAGA TACTTCTGGG AGAAGCTGCG CTACGCCATC 
CCAATTGAGC TGTCGCCCCG TGGAAACAAC TACACATTGG ACCATCATGA GCGCTTCAAG 
CAGCCAGTGT CGCCGGGCAT GATCTTCCGC CAGGCGCCAC CACCGCCAGC CTACTACTGC 
ACCGAGGAAA TGGAGGCGAA CTATAGCTCA GCCACCACGG CCACGCCATC ACCACGCCCA 
ACGAGACCAG GAGGAGCCGC TAGGATCGTG GACTCTATGC CCATGCCGAT GCGTCCGCCC 
TCAGAGCACA TCTACCACAG CATCGAGTCG GAGTACAGTG CGTATGATCA GCATGAGGCG 
CTGTCGATGA TTCCCACGGG CCTAATGCAT CAGCACCAGC AGCAGCAGTT GCGTCTGCAT 
CAGCAACAAC AACAGCAGCA GCAACAGCGC TTGCTGCAAC CGCAGTTCCG GGCGATGCCG 
CAGCAGGCGA TTCCCGCTCC TTCCGCGCCG GTGCACCTGC GCAGCGGCAG TGGTTTGAGC 
CAGGCCAGCA CCAGCACTCA GTCAACGGCC CAGGCCTCCA CATCCGCAGC AGCGGCGCAG 
CAGCAACAGC AACAGCAGCA GCAACAGGCA GCTGGCAGTG AAGCGGCCAA TAAGAACGGC 
CAAGCTTTCC TGGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476814.1
CDS	375..4532
Translation	MPATSSIITIIAVAACLLLLVADAHAQQQCNWQYGLTTMDIRCSVRALESGTGTPLDLQVAEAAGRLDLQCSQELLHASELAPGLFRQLQKLSELRIDACKLQRVPPNAFEGLMSLKRLTLESHNAVWGPGKTLELHGQSFQGLKELSELHLGDNNIRQLPEGVWCSMPSLQLLNLTQNRIRSAEFLGFSEKLCAGSALSNANGAVSGGSELQTLDVSFNELRSLPDAWGASRLRRLQTLSLQHNNISTLAPNALAGLSSLRVLNISYNHLVSLPSEAFAGNKELRELHLQGNDLYELPKGLLHRLEQLLVLDLSGNQLTSHHVDNSTFAGLIRLIVLNLSNNALTRIGSKTFKELYFLQILDMRNNSIGHIEEGAFLPLYNLHTLNLAENRLHTLDNRIFNGLYVLTKLTLNNNLVSIVESQAFRNCSDLKELDLSSNQLTEVPEAVQDLSMLKTLDLGENQISEFKNNTFRNLNQLTGLRLIDNRIGNITVGMFQDLPRLSVLNLAKNRIQSIERGAFDKNTEIEAIRLDKNFLTDINGIFATLASLLWLNLSENHLVWFDYAFIPSNLKWLDIHGNYIEALGNYYKLQEEIRVTTLDASHNRITEIGAMSVPNSIELLFINNNIIGQIQANTFVDKTRLARVDLYANVLSKISLNALRVAPVSAEKPVPEFYLGGNPFECDCSMEWLQRINNLTTRQHPHVVDLGNIECLMPHSRSAPLRPLASLSASDFVCKYESHCPPTCHCCEYEQCECEVICPGNCSCFHDATWATNIVDCGRQDLAALPNRIPQDVSDLYLDGNNMPELEVGHLTGRRNLRALYLNASNLMTLQNGSLAQLVNLRVLHLENNKLTALEGTEFRSLGLLRELYLHNNMLTHISNATFEPLVSLEVLRLDNNRLSSLPHLQYRHSLQGLTLGRNAWSCRCQQLRELAQFVSDNAMVVRDAHDIYCLDAGIKRELELIGNLANGPDCSDLLDASASNISSSQDLAGGYRLPLLAAVLVLIFLVVVLIIVFVFRESVRMWLFAHYGVRVCEPRFEDAGKLYDAIILHSEKDYEFVCRNIAAELEHGRPPFRLCIQQRDLPPQASHLQLVEGARASRKIILVLTRNLLATEWNRIEFRNAFHESLRGLAQKLVIIEETSVSAEAEDVAELSPYLKSVPSNRLLTCDRYFWEKLRYAIPIELSPRGNNYTLDHHERFKQPVSPGMIFRQAPPPPAYYCTEEMEANYSSATTATPSPRPTRPGGAARIVDSMPMPMRPPSEHIYHSIESEYSAYDQHEALSMIPTGLMHQHQQQQLRLHQQQQQQQQQRLLQPQFRAMPQQAIPAPSAPVHLRSGSGLSQASTSTQSTAQASTSAAAAQQQQQQQQQQAAGSEAANKNGQAFLV

Target ORF information:

RefSeq Version	NM_057466.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster 18 wheeler (18w), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057466.2

ORF Insert Sequence:

ATGCCAGCCA CATCTTCCAT CATCACCATC ATCGCAGTCG CTGCCTGCCT GTTGCTTCTT 
GTGGCTGATG CGCATGCGCA GCAGCAGTGC AACTGGCAGT ACGGCCTCAC CACCATGGAC 
ATCCGGTGCA GTGTCCGCGC TCTGGAGTCC GGCACCGGAA CGCCACTTGA CCTCCAGGTG 
GCGGAGGCGG CCGGCCGCCT GGATCTGCAG TGCAGCCAGG AGCTCCTGCA TGCCAGTGAA 
CTGGCACCGG GACTCTTCCG GCAACTGCAG AAGCTCTCGG AGCTGCGAAT CGATGCCTGC 
AAACTGCAGC GAGTGCCTCC AAATGCATTT GAGGGTCTGA TGTCGCTCAA GCGACTCACC 
CTGGAGTCAC ACAACGCGGT CTGGGGTCCG GGAAAGACCC TGGAGTTGCA CGGCCAGTCG 
TTCCAGGGAC TGAAGGAGCT GTCCGAGCTG CACTTGGGCG ACAACAATAT CCGTCAGTTG 
CCCGAGGGCG TCTGGTGCTC CATGCCCAGC CTGCAACTGC TCAATCTCAC CCAGAACCGG 
ATACGTTCCG CCGAGTTCCT AGGCTTCTCC GAGAAACTCT GTGCCGGCTC AGCCCTGAGC 
AATGCCAATG GAGCAGTTAG CGGAGGCTCA GAGCTGCAGA CATTGGATGT CTCGTTCAAC 
GAGCTGCGTT CTTTGCCGGA TGCCTGGGGA GCGTCCCGAT TGAGGCGCCT GCAGACCCTC 
AGCCTGCAAC ACAACAACAT CAGCACCCTG GCTCCCAATG CCCTGGCCGG TCTGAGTTCG 
CTGCGAGTGC TGAATATCTC GTACAACCAC CTCGTTTCCC TGCCCTCAGA AGCCTTTGCC 
GGCAACAAGG AGTTGAGGGA ACTGCATCTG CAGGGCAATG ATCTGTACGA ATTGCCCAAA 
GGTCTGTTGC ACCGTCTGGA GCAGCTGTTA GTTCTCGATC TGAGTGGCAA TCAGCTGACC 
AGCCACCATG TTGACAACAG CACCTTCGCC GGTCTGATCC GCCTGATTGT GCTGAATCTG 
TCGAACAATG CCCTCACCCG CATTGGATCC AAAACCTTCA AGGAGCTGTA CTTCCTGCAG 
ATTCTGGACA TGCGAAACAA CTCCATTGGC CACATTGAGG AGGGCGCATT CCTGCCCCTG 
TACAATCTGC ACACCCTCAA TCTCGCCGAG AATCGCCTGC ACACACTGGA CAACCGCATC 
TTCAACGGAT TGTATGTGCT GACGAAACTC ACGCTAAACA ACAATCTTGT TAGCATTGTG 
GAGTCGCAAG CCTTCCGCAA CTGTTCCGAT CTGAAGGAAC TGGATCTCAG CTCCAATCAG 
CTGACTGAAG TGCCCGAGGC TGTGCAAGAT TTGAGCATGC TCAAGACTCT CGATCTCGGA 
GAGAATCAGA TCTCGGAATT CAAGAACAAC ACCTTCCGCA ATCTCAATCA GCTGACGGGT 
CTGCGATTGA TCGACAATCG CATCGGCAAC ATCACCGTAG GCATGTTTCA GGATCTGCCC 
CGTTTGAGTG TTCTCAACCT GGCCAAGAAC CGCATCCAGA GCATCGAGCG TGGAGCCTTC 
GACAAAAACA CCGAGATCGA GGCGATCCGA TTGGACAAGA ACTTCCTCAC CGACATCAAT 
GGAATCTTTG CCACTCTCGC CTCCTTGCTG TGGCTGAATC TCTCCGAGAA TCACCTTGTT 
TGGTTCGATT ACGCCTTCAT CCCATCGAAC TTGAAATGGC TGGATATCCA TGGAAACTAC 
ATCGAAGCCC TGGGCAACTA CTACAAACTG CAAGAGGAGA TTCGTGTGAC CACGCTGGAT 
GCCTCTCACA ACCGCATCAC GGAGATTGGC GCCATGTCGG TGCCGAATTC CATCGAGCTG 
CTGTTCATCA ACAACAACAT CATTGGACAG ATCCAGGCCA ACACCTTTGT GGACAAGACG 
CGTCTGGCAC GCGTGGATCT GTATGCCAAT GTGCTGTCCA AAATCTCGCT TAATGCTCTA 
CGCGTAGCCC CAGTTTCAGC GGAGAAACCA GTTCCCGAGT TTTACCTGGG CGGCAATCCC 
TTTGAGTGCG ATTGCTCCAT GGAGTGGCTG CAGAGGATTA ACAATCTGAC CACCCGCCAG 
CATCCTCATG TTGTGGATCT GGGAAACATC GAGTGCCTGA TGCCGCACAG CCGCAGTGCT 
CCACTGCGTC CGCTGGCCAG TCTCTCCGCC TCCGACTTTG TGTGCAAGTA TGAGAGCCAC 
TGCCCGCCCA CGTGCCACTG CTGCGAGTAC GAACAGTGCG AGTGTGAAGT GATCTGCCCT 
GGAAACTGCA GCTGCTTCCA CGACGCCACC TGGGCCACCA ACATCGTGGA CTGCGGTCGC 
CAGGATCTGG CCGCCCTGCC CAACCGTATC CCTCAGGATG TGAGCGATCT CTACTTGGAT 
GGCAACAACA TGCCCGAGCT GGAGGTTGGC CACCTAACCG GTCGTCGCAA TCTGCGGGCT 
CTCTACCTGA ACGCCTCCAA TCTGATGACG CTGCAGAACG GAAGTCTGGC CCAGTTGGTC 
AATCTTCGAG TGCTCCACTT GGAGAACAAC AAACTAACCG CCCTGGAAGG CACCGAGTTC 
CGCTCCCTGG GACTTTTGCG GGAGCTGTAT CTGCACAACA ACATGCTGAC CCACATCTCG 
AATGCCACCT TTGAGCCACT GGTGTCCCTG GAGGTGCTGC GTCTGGACAA CAACCGCCTG 
AGTTCCCTGC CCCATTTGCA ATATCGTCAC AGCCTCCAGG GACTGACGCT GGGCAGAAAT 
GCCTGGTCAT GCCGCTGCCA GCAGTTGAGG GAATTGGCGC AGTTTGTCTC CGATAACGCC 
ATGGTGGTGC GGGATGCCCA CGATATCTAC TGCTTGGATG CGGGCATCAA GCGCGAGCTG 
GAACTGATTG GCAACCTGGC CAACGGACCC GATTGTTCCG ACCTTCTGGA TGCCAGTGCC 
AGCAATATCA GCTCCTCGCA GGATTTGGCC GGAGGCTATA GGCTGCCCTT GTTGGCCGCT 
GTGCTAGTGC TGATCTTCCT GGTCGTGGTG CTCATCATTG TCTTCGTCTT CCGCGAGAGC 
GTGCGCATGT GGCTCTTCGC CCACTACGGA GTCAGGGTGT GCGAGCCCCG CTTCGAGGAT 
GCCGGCAAAC TGTACGACGC CATCATCCTG CACTCGGAGA AGGACTACGA GTTTGTGTGC 
CGCAACATCG CCGCCGAACT GGAGCATGGT CGACCACCCT TCCGGCTCTG CATTCAGCAG 
CGAGATCTGC CTCCCCAGGC ATCCCACCTT CAGCTGGTGG AGGGAGCAAG GGCGTCGAGG 
AAGATCATCC TGGTGCTGAC CCGCAATCTC TTGGCCACCG AATGGAATCG CATTGAGTTC 
CGTAATGCTT TCCACGAGTC CCTGAGGGGC TTGGCCCAGA AGCTGGTGAT CATCGAGGAG 
ACAAGTGTTT CCGCCGAGGC CGAGGACGTT GCCGAGTTGT CGCCGTACTT GAAATCGGTA 
CCCTCCAACC GACTGCTGAC CTGCGACAGA TACTTCTGGG AGAAGCTGCG CTACGCCATC 
CCAATTGAGC TGTCGCCCCG TGGAAACAAC TACACATTGG ACCATCATGA GCGCTTCAAG 
CAGCCAGTGT CGCCGGGCAT GATCTTCCGC CAGGCGCCAC CACCGCCAGC CTACTACTGC 
ACCGAGGAAA TGGAGGCGAA CTATAGCTCA GCCACCACGG CCACGCCATC ACCACGCCCA 
ACGAGACCAG GAGGAGCCGC TAGGATCGTG GACTCTATGC CCATGCCGAT GCGTCCGCCC 
TCAGAGCACA TCTACCACAG CATCGAGTCG GAGTACAGTG CGTATGATCA GCATGAGGCG 
CTGTCGATGA TTCCCACGGG CCTAATGCAT CAGCACCAGC AGCAGCAGTT GCGTCTGCAT 
CAGCAACAAC AACAGCAGCA GCAACAGCGC TTGCTGCAAC CGCAGTTCCG GGCGATGCCG 
CAGCAGGCGA TTCCCGCTCC TTCCGCGCCG GTGCACCTGC GCAGCGGCAG TGGTTTGAGC 
CAGGCCAGCA CCAGCACTCA GTCAACGGCC CAGGCCTCCA CATCCGCAGC AGCGGCGCAG 
CAGCAACAGC AACAGCAGCA GCAACAGGCA GCTGGCAGTG AAGCGGCCAA TAAGAACGGC 
CAAGCTTTCC TGGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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18w cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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