CG6424 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG6424
Entrez Gene ID	37012
Full Name	CG6424 gene product from transcript CG6424-RA
Synonyms	BcDNA:GH05095,Dmel\CG6424,GH05095,anon-WO0118547.96,anon-WO0153538.32,anon-WO0153538.33,anon-WO0153538.34
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 54E1-54E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_137405.4	NP_611249.2	uncharacterized protein, isoform B
NM_001169710.2	NP_001163181.1	uncharacterized protein, isoform C
NM_166246.3	NP_725709.1	uncharacterized protein, isoform A
NM_001299617.1	NP_001286546.1	uncharacterized protein, isoform D

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG6424 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG6424 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa10372	NM_001299617.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant D (CG6424), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa10372	NM_137405.4 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG6424), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa10372	NM_001169710.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant C (CG6424), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa10372	NM_166246.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG6424), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG6424 ( NM_137405.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611249.2 Drosophila melanogaster uncharacterized protein, transcript variant B (CG6424), mRNA.

Price & Availability↑

CloneID	OFa10372
Clone ID Related Accession (Same CDS sequence)	NM_137405.4 , NM_001169710.2 , NM_166246.3 , NM_001299617.1
Accession Version	NM_137405.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137405.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGCCATC CCACAACTGG GCTGAACGAA GACGACCTGA GTCGTCTCAC TGGTTCATCA 
TCGTCGACGG CTTCGTGCGG CAGCGCAGCA TCCTCATCAT CGTCCTCATC CTCGTCGTCC 
TCGAATCCCG CTGACCGACA TGAGGTGGTC TGCAGTCCGG AAAAGCCAAC GCCGACGGGC 
ACGGACAAGA GCCAACTGAG CAGCGGCTTT AACTCCACTT ACCTGCCGGA AATCATACAT 
CCGGCTCCGG AATGGCAGAA GTCATCGCGC AAGCGCAAGG AACGCCTGGA CAGCACCTCG 
GCCTTTGGCC AGGATCGAAA GTTACAGCGC AGCAACAGCG AGGAGCTGCT CACAGAACCA 
GCTGCCATTG TGTCTTCCAC AGCCCAGGCA GCAGCCGAAC TTCTGGAGGC CCAGTGTGAG 
GTTATAAGAC GGGTTTCTTC GGAGGATTTC AAACGCACGG CCAGTTACGC CAACTATCGG 
CAGGAGTTCG AAAGGGAGCA GGAGGAGTGC TCGGAGCTGG GTGAGAACAA TGCCTCGTTG 
GCCAACCTGC CGACAGGAGA AGCAGCCAAA GAGCGTCCTC GTCTGGAGAC AAGCCCCGCT 
CGCCAGGCAC CAAAGGAGGC TAGCGATGAC TCCGAGTGCG AGCACGAAAG GCGACGCAGC 
AGTGAGCGTT TCTGCAAATC CCGCCAGCCG CCGGTGCGCA AGTCTGGCGT GGCCAAGAAA 
GTCGGCGGAG GCAGTGGCTC CAAATATCTG CCATCTCGAC GGGATGAGCA AAGCGCCTAC 
AAGTACGACT TGTCGGCTTT GAAGTACGAA CGCAGAGGTT TCATTAGCAG CAGGAAACAG 
CAGCAGAATC AAACACAAGC AATGCAGTCT GCTGCCGATC ACAACGACAA CAACTTGATA 
GACTTCCATC AGCAGCAGAA GGAGCTGCAG TCGAAGCGGA GTGCCAGTAT TTCCCCCACA 
CCCACCACGA GCTCCTCGGC GGGCAGCGAT GACAGCACGC CCACTTCCAA TGCACCCGCG 
CCCATCAAGG CCAAACCCCA GAGGCAGCAG CAGAGCCTGG ATCTCACCTC CGCGCAGGAG 
TCCCTGCCGT GGGAGCGTGG AGATGAACCG GCACCCATTC TGAGCAGAAG ATACGCCCAC 
TCCCGACCAC ATATCGGTGG CAATCTCGAT GCGGCCGCCA AATTGGCCAA GGTCATGCCA 
TATCCCCAGC AGCTGCCCAA ACAGGCATCC ACCGTGCAGC TGCACTCGGA CATCACCGAC 
ATGGACTGCT GCCTGGAGCC CATGGATGCC GTGCGAGCGT TCAGTTCGCT GGAGAATATG 
CGAACCCGCG ACGTTATGCA ACAAGTGTTG CCCGCCATGA TGGGTGCATT TCAAACGCCC 
GAGGATCGAG TGAAGGCCAT CAACAGACGC GTTACTGTGC TCAAGAAGAA ACTGGTTCAG 
CTGGAGGAAT CCCTGGAGCA GCGACTGGGC TATCGGCCAT CTCAGGCGGA GCGATTGAAC 
GACAAGTACA TGAAGAATGC CCTGGCGGAG TTGAGCAAGC TGCGCAAGGA ACGGCACGAG 
CTCAAGACTG ATCCCATCGC AGCTCTGGGT CTGAAGGTGG GCAGCGGCGG TGGCGCCGGC 
ATCGGTGGCC AGGAGGTCGC CAAGAAGCTG GAGCGCATGA AAGCCACACT CGCTGAAATC 
GAGCAGAATT TGAACGAGAA ACGCGTGAAT GGTCATCGTT CTGAGCAGCT AGAGGATCTG 
AATGCCGACC AACTGGTGCA GGAGAAGAGC GCCGTGCAGC ATGGACTGCT GTACTTTGAG 
AGCCTCTATG GCCATCCCAT TACCAAGGAG GAGCGCGATG CTGCCCGGCC GCTCTACGAT 
CGCTACCGGC AGCTGAAGCG TCTGGTGTCG CGCACCGTGA TGTTTGGAGC GGGCACAGGG 
ATACCCGAGT TGCCCACAAT CCTGGAGCAC GAGGCCATGG TGTTTGAGGC CACTTCCACA 
CCGCTGCAGT ATTCGTCGAA CAACAGCACA GAGACCACCA ACTCGCCCAG CGACTCGAAT 
GCCCCGAATA CTCTGACCCA GAATGCCTCA TCCGACGAGT CGACGACCAC CACCACGTTG 
ACGGGTCCGG CAACGGGAAA TTCGGCCACA AGCCAGGAGA ACCAGGCCGC CAGCGAGAAT 
ATCGCCGCAC TGAGTGTGGA CCAACTCTGG GAGCAGCTAG ATCGAGCACG CGATGAGAAA 
TCCCTGCTAA AATCCACCAT TCGGGAGTAC GAGTCGCTGT TCGAGGAGCA GAATGGACGC 
AAGATGCTCA AGCAGGATCG TCGCTCACTG GAGACGGAAA CCTATGCCCA GTACAAGGAG 
AAGAAGGCCA AGGTCAGACT ACTGCAGGCC CTGATCAAGA AGCATATTGG GCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611249.2
CDS	795..3191
Translation	MRHPTTGLNEDDLSRLTGSSSSTASCGSAASSSSSSSSSSSNPADRHEVVCSPEKPTPTGTDKSQLSSGFNSTYLPEIIHPAPEWQKSSRKRKERLDSTSAFGQDRKLQRSNSEELLTEPAAIVSSTAQAAAELLEAQCEVIRRVSSEDFKRTASYANYRQEFEREQEECSELGENNASLANLPTGEAAKERPRLETSPARQAPKEASDDSECEHERRRSSERFCKSRQPPVRKSGVAKKVGGGSGSKYLPSRRDEQSAYKYDLSALKYERRGFISSRKQQQNQTQAMQSAADHNDNNLIDFHQQQKELQSKRSASISPTPTTSSSAGSDDSTPTSNAPAPIKAKPQRQQQSLDLTSAQESLPWERGDEPAPILSRRYAHSRPHIGGNLDAAAKLAKVMPYPQQLPKQASTVQLHSDITDMDCCLEPMDAVRAFSSLENMRTRDVMQQVLPAMMGAFQTPEDRVKAINRRVTVLKKKLVQLEESLEQRLGYRPSQAERLNDKYMKNALAELSKLRKERHELKTDPIAALGLKVGSGGGAGIGGQEVAKKLERMKATLAEIEQNLNEKRVNGHRSEQLEDLNADQLVQEKSAVQHGLLYFESLYGHPITKEERDAARPLYDRYRQLKRLVSRTVMFGAGTGIPELPTILEHEAMVFEATSTPLQYSSNNSTETTNSPSDSNAPNTLTQNASSDESTTTTTLTGPATGNSATSQENQAASENIAALSVDQLWEQLDRARDEKSLLKSTIREYESLFEEQNGRKMLKQDRRSLETETYAQYKEKKAKVRLLQALIKKHIGH

Target ORF information:

RefSeq Version	NM_137405.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG6424), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137405.4

ORF Insert Sequence:

ATGCGCCATC CCACAACTGG GCTGAACGAA GACGACCTGA GTCGTCTCAC TGGTTCATCA 
TCGTCGACGG CTTCGTGCGG CAGCGCAGCA TCCTCATCAT CGTCCTCATC CTCGTCGTCC 
TCGAATCCCG CTGACCGACA TGAGGTGGTC TGCAGTCCGG AAAAGCCAAC GCCGACGGGC 
ACGGACAAGA GCCAACTGAG CAGCGGCTTT AACTCCACTT ACCTGCCGGA AATCATACAT 
CCGGCTCCGG AATGGCAGAA GTCATCGCGC AAGCGCAAGG AACGCCTGGA CAGCACCTCG 
GCCTTTGGCC AGGATCGAAA GTTACAGCGC AGCAACAGCG AGGAGCTGCT CACAGAACCA 
GCTGCCATTG TGTCTTCCAC AGCCCAGGCA GCAGCCGAAC TTCTGGAGGC CCAGTGTGAG 
GTTATAAGAC GGGTTTCTTC GGAGGATTTC AAACGCACGG CCAGTTACGC CAACTATCGG 
CAGGAGTTCG AAAGGGAGCA GGAGGAGTGC TCGGAGCTGG GTGAGAACAA TGCCTCGTTG 
GCCAACCTGC CGACAGGAGA AGCAGCCAAA GAGCGTCCTC GTCTGGAGAC AAGCCCCGCT 
CGCCAGGCAC CAAAGGAGGC TAGCGATGAC TCCGAGTGCG AGCACGAAAG GCGACGCAGC 
AGTGAGCGTT TCTGCAAATC CCGCCAGCCG CCGGTGCGCA AGTCTGGCGT GGCCAAGAAA 
GTCGGCGGAG GCAGTGGCTC CAAATATCTG CCATCTCGAC GGGATGAGCA AAGCGCCTAC 
AAGTACGACT TGTCGGCTTT GAAGTACGAA CGCAGAGGTT TCATTAGCAG CAGGAAACAG 
CAGCAGAATC AAACACAAGC AATGCAGTCT GCTGCCGATC ACAACGACAA CAACTTGATA 
GACTTCCATC AGCAGCAGAA GGAGCTGCAG TCGAAGCGGA GTGCCAGTAT TTCCCCCACA 
CCCACCACGA GCTCCTCGGC GGGCAGCGAT GACAGCACGC CCACTTCCAA TGCACCCGCG 
CCCATCAAGG CCAAACCCCA GAGGCAGCAG CAGAGCCTGG ATCTCACCTC CGCGCAGGAG 
TCCCTGCCGT GGGAGCGTGG AGATGAACCG GCACCCATTC TGAGCAGAAG ATACGCCCAC 
TCCCGACCAC ATATCGGTGG CAATCTCGAT GCGGCCGCCA AATTGGCCAA GGTCATGCCA 
TATCCCCAGC AGCTGCCCAA ACAGGCATCC ACCGTGCAGC TGCACTCGGA CATCACCGAC 
ATGGACTGCT GCCTGGAGCC CATGGATGCC GTGCGAGCGT TCAGTTCGCT GGAGAATATG 
CGAACCCGCG ACGTTATGCA ACAAGTGTTG CCCGCCATGA TGGGTGCATT TCAAACGCCC 
GAGGATCGAG TGAAGGCCAT CAACAGACGC GTTACTGTGC TCAAGAAGAA ACTGGTTCAG 
CTGGAGGAAT CCCTGGAGCA GCGACTGGGC TATCGGCCAT CTCAGGCGGA GCGATTGAAC 
GACAAGTACA TGAAGAATGC CCTGGCGGAG TTGAGCAAGC TGCGCAAGGA ACGGCACGAG 
CTCAAGACTG ATCCCATCGC AGCTCTGGGT CTGAAGGTGG GCAGCGGCGG TGGCGCCGGC 
ATCGGTGGCC AGGAGGTCGC CAAGAAGCTG GAGCGCATGA AAGCCACACT CGCTGAAATC 
GAGCAGAATT TGAACGAGAA ACGCGTGAAT GGTCATCGTT CTGAGCAGCT AGAGGATCTG 
AATGCCGACC AACTGGTGCA GGAGAAGAGC GCCGTGCAGC ATGGACTGCT GTACTTTGAG 
AGCCTCTATG GCCATCCCAT TACCAAGGAG GAGCGCGATG CTGCCCGGCC GCTCTACGAT 
CGCTACCGGC AGCTGAAGCG TCTGGTGTCG CGCACCGTGA TGTTTGGAGC GGGCACAGGG 
ATACCCGAGT TGCCCACAAT CCTGGAGCAC GAGGCCATGG TGTTTGAGGC CACTTCCACA 
CCGCTGCAGT ATTCGTCGAA CAACAGCACA GAGACCACCA ACTCGCCCAG CGACTCGAAT 
GCCCCGAATA CTCTGACCCA GAATGCCTCA TCCGACGAGT CGACGACCAC CACCACGTTG 
ACGGGTCCGG CAACGGGAAA TTCGGCCACA AGCCAGGAGA ACCAGGCCGC CAGCGAGAAT 
ATCGCCGCAC TGAGTGTGGA CCAACTCTGG GAGCAGCTAG ATCGAGCACG CGATGAGAAA 
TCCCTGCTAA AATCCACCAT TCGGGAGTAC GAGTCGCTGT TCGAGGAGCA GAATGGACGC 
AAGATGCTCA AGCAGGATCG TCGCTCACTG GAGACGGAAA CCTATGCCCA GTACAAGGAG 
AAGAAGGCCA AGGTCAGACT ACTGCAGGCC CTGATCAAGA AGCATATTGG GCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG6424 ( NM_001169710.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163181.1 Drosophila melanogaster uncharacterized protein, transcript variant C (CG6424), mRNA.

Price & Availability↑

CloneID	OFa10372
Clone ID Related Accession (Same CDS sequence)	NM_137405.4 , NM_001169710.2 , NM_166246.3 , NM_001299617.1
Accession Version	NM_001169710.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001169710.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGCCATC CCACAACTGG GCTGAACGAA GACGACCTGA GTCGTCTCAC TGGTTCATCA 
TCGTCGACGG CTTCGTGCGG CAGCGCAGCA TCCTCATCAT CGTCCTCATC CTCGTCGTCC 
TCGAATCCCG CTGACCGACA TGAGGTGGTC TGCAGTCCGG AAAAGCCAAC GCCGACGGGC 
ACGGACAAGA GCCAACTGAG CAGCGGCTTT AACTCCACTT ACCTGCCGGA AATCATACAT 
CCGGCTCCGG AATGGCAGAA GTCATCGCGC AAGCGCAAGG AACGCCTGGA CAGCACCTCG 
GCCTTTGGCC AGGATCGAAA GTTACAGCGC AGCAACAGCG AGGAGCTGCT CACAGAACCA 
GCTGCCATTG TGTCTTCCAC AGCCCAGGCA GCAGCCGAAC TTCTGGAGGC CCAGTGTGAG 
GTTATAAGAC GGGTTTCTTC GGAGGATTTC AAACGCACGG CCAGTTACGC CAACTATCGG 
CAGGAGTTCG AAAGGGAGCA GGAGGAGTGC TCGGAGCTGG GTGAGAACAA TGCCTCGTTG 
GCCAACCTGC CGACAGGAGA AGCAGCCAAA GAGCGTCCTC GTCTGGAGAC AAGCCCCGCT 
CGCCAGGCAC CAAAGGAGGC TAGCGATGAC TCCGAGTGCG AGCACGAAAG GCGACGCAGC 
AGTGAGCGTT TCTGCAAATC CCGCCAGCCG CCGGTGCGCA AGTCTGGCGT GGCCAAGAAA 
GTCGGCGGAG GCAGTGGCTC CAAATATCTG CCATCTCGAC GGGATGAGCA AAGCGCCTAC 
AAGTACGACT TGTCGGCTTT GAAGTACGAA CGCAGAGGTT TCATTAGCAG CAGGAAACAG 
CAGCAGAATC AAACACAAGC AATGCAGTCT GCTGCCGATC ACAACGACAA CAACTTGATA 
GACTTCCATC AGCAGCAGAA GGAGCTGCAG TCGAAGCGGA GTGCCAGTAT TTCCCCCACA 
CCCACCACGA GCTCCTCGGC GGGCAGCGAT GACAGCACGC CCACTTCCAA TGCACCCGCG 
CCCATCAAGG CCAAACCCCA GAGGCAGCAG CAGAGCCTGG ATCTCACCTC CGCGCAGGAG 
TCCCTGCCGT GGGAGCGTGG AGATGAACCG GCACCCATTC TGAGCAGAAG ATACGCCCAC 
TCCCGACCAC ATATCGGTGG CAATCTCGAT GCGGCCGCCA AATTGGCCAA GGTCATGCCA 
TATCCCCAGC AGCTGCCCAA ACAGGCATCC ACCGTGCAGC TGCACTCGGA CATCACCGAC 
ATGGACTGCT GCCTGGAGCC CATGGATGCC GTGCGAGCGT TCAGTTCGCT GGAGAATATG 
CGAACCCGCG ACGTTATGCA ACAAGTGTTG CCCGCCATGA TGGGTGCATT TCAAACGCCC 
GAGGATCGAG TGAAGGCCAT CAACAGACGC GTTACTGTGC TCAAGAAGAA ACTGGTTCAG 
CTGGAGGAAT CCCTGGAGCA GCGACTGGGC TATCGGCCAT CTCAGGCGGA GCGATTGAAC 
GACAAGTACA TGAAGAATGC CCTGGCGGAG TTGAGCAAGC TGCGCAAGGA ACGGCACGAG 
CTCAAGACTG ATCCCATCGC AGCTCTGGGT CTGAAGGTGG GCAGCGGCGG TGGCGCCGGC 
ATCGGTGGCC AGGAGGTCGC CAAGAAGCTG GAGCGCATGA AAGCCACACT CGCTGAAATC 
GAGCAGAATT TGAACGAGAA ACGCGTGAAT GGTCATCGTT CTGAGCAGCT AGAGGATCTG 
AATGCCGACC AACTGGTGCA GGAGAAGAGC GCCGTGCAGC ATGGACTGCT GTACTTTGAG 
AGCCTCTATG GCCATCCCAT TACCAAGGAG GAGCGCGATG CTGCCCGGCC GCTCTACGAT 
CGCTACCGGC AGCTGAAGCG TCTGGTGTCG CGCACCGTGA TGTTTGGAGC GGGCACAGGG 
ATACCCGAGT TGCCCACAAT CCTGGAGCAC GAGGCCATGG TGTTTGAGGC CACTTCCACA 
CCGCTGCAGT ATTCGTCGAA CAACAGCACA GAGACCACCA ACTCGCCCAG CGACTCGAAT 
GCCCCGAATA CTCTGACCCA GAATGCCTCA TCCGACGAGT CGACGACCAC CACCACGTTG 
ACGGGTCCGG CAACGGGAAA TTCGGCCACA AGCCAGGAGA ACCAGGCCGC CAGCGAGAAT 
ATCGCCGCAC TGAGTGTGGA CCAACTCTGG GAGCAGCTAG ATCGAGCACG CGATGAGAAA 
TCCCTGCTAA AATCCACCAT TCGGGAGTAC GAGTCGCTGT TCGAGGAGCA GAATGGACGC 
AAGATGCTCA AGCAGGATCG TCGCTCACTG GAGACGGAAA CCTATGCCCA GTACAAGGAG 
AAGAAGGCCA AGGTCAGACT ACTGCAGGCC CTGATCAAGA AGCATATTGG GCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163181.1
CDS	486..2882
Translation	MRHPTTGLNEDDLSRLTGSSSSTASCGSAASSSSSSSSSSSNPADRHEVVCSPEKPTPTGTDKSQLSSGFNSTYLPEIIHPAPEWQKSSRKRKERLDSTSAFGQDRKLQRSNSEELLTEPAAIVSSTAQAAAELLEAQCEVIRRVSSEDFKRTASYANYRQEFEREQEECSELGENNASLANLPTGEAAKERPRLETSPARQAPKEASDDSECEHERRRSSERFCKSRQPPVRKSGVAKKVGGGSGSKYLPSRRDEQSAYKYDLSALKYERRGFISSRKQQQNQTQAMQSAADHNDNNLIDFHQQQKELQSKRSASISPTPTTSSSAGSDDSTPTSNAPAPIKAKPQRQQQSLDLTSAQESLPWERGDEPAPILSRRYAHSRPHIGGNLDAAAKLAKVMPYPQQLPKQASTVQLHSDITDMDCCLEPMDAVRAFSSLENMRTRDVMQQVLPAMMGAFQTPEDRVKAINRRVTVLKKKLVQLEESLEQRLGYRPSQAERLNDKYMKNALAELSKLRKERHELKTDPIAALGLKVGSGGGAGIGGQEVAKKLERMKATLAEIEQNLNEKRVNGHRSEQLEDLNADQLVQEKSAVQHGLLYFESLYGHPITKEERDAARPLYDRYRQLKRLVSRTVMFGAGTGIPELPTILEHEAMVFEATSTPLQYSSNNSTETTNSPSDSNAPNTLTQNASSDESTTTTTLTGPATGNSATSQENQAASENIAALSVDQLWEQLDRARDEKSLLKSTIREYESLFEEQNGRKMLKQDRRSLETETYAQYKEKKAKVRLLQALIKKHIGH

Target ORF information:

RefSeq Version	NM_001169710.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant C (CG6424), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169710.2

ORF Insert Sequence:

ATGCGCCATC CCACAACTGG GCTGAACGAA GACGACCTGA GTCGTCTCAC TGGTTCATCA 
TCGTCGACGG CTTCGTGCGG CAGCGCAGCA TCCTCATCAT CGTCCTCATC CTCGTCGTCC 
TCGAATCCCG CTGACCGACA TGAGGTGGTC TGCAGTCCGG AAAAGCCAAC GCCGACGGGC 
ACGGACAAGA GCCAACTGAG CAGCGGCTTT AACTCCACTT ACCTGCCGGA AATCATACAT 
CCGGCTCCGG AATGGCAGAA GTCATCGCGC AAGCGCAAGG AACGCCTGGA CAGCACCTCG 
GCCTTTGGCC AGGATCGAAA GTTACAGCGC AGCAACAGCG AGGAGCTGCT CACAGAACCA 
GCTGCCATTG TGTCTTCCAC AGCCCAGGCA GCAGCCGAAC TTCTGGAGGC CCAGTGTGAG 
GTTATAAGAC GGGTTTCTTC GGAGGATTTC AAACGCACGG CCAGTTACGC CAACTATCGG 
CAGGAGTTCG AAAGGGAGCA GGAGGAGTGC TCGGAGCTGG GTGAGAACAA TGCCTCGTTG 
GCCAACCTGC CGACAGGAGA AGCAGCCAAA GAGCGTCCTC GTCTGGAGAC AAGCCCCGCT 
CGCCAGGCAC CAAAGGAGGC TAGCGATGAC TCCGAGTGCG AGCACGAAAG GCGACGCAGC 
AGTGAGCGTT TCTGCAAATC CCGCCAGCCG CCGGTGCGCA AGTCTGGCGT GGCCAAGAAA 
GTCGGCGGAG GCAGTGGCTC CAAATATCTG CCATCTCGAC GGGATGAGCA AAGCGCCTAC 
AAGTACGACT TGTCGGCTTT GAAGTACGAA CGCAGAGGTT TCATTAGCAG CAGGAAACAG 
CAGCAGAATC AAACACAAGC AATGCAGTCT GCTGCCGATC ACAACGACAA CAACTTGATA 
GACTTCCATC AGCAGCAGAA GGAGCTGCAG TCGAAGCGGA GTGCCAGTAT TTCCCCCACA 
CCCACCACGA GCTCCTCGGC GGGCAGCGAT GACAGCACGC CCACTTCCAA TGCACCCGCG 
CCCATCAAGG CCAAACCCCA GAGGCAGCAG CAGAGCCTGG ATCTCACCTC CGCGCAGGAG 
TCCCTGCCGT GGGAGCGTGG AGATGAACCG GCACCCATTC TGAGCAGAAG ATACGCCCAC 
TCCCGACCAC ATATCGGTGG CAATCTCGAT GCGGCCGCCA AATTGGCCAA GGTCATGCCA 
TATCCCCAGC AGCTGCCCAA ACAGGCATCC ACCGTGCAGC TGCACTCGGA CATCACCGAC 
ATGGACTGCT GCCTGGAGCC CATGGATGCC GTGCGAGCGT TCAGTTCGCT GGAGAATATG 
CGAACCCGCG ACGTTATGCA ACAAGTGTTG CCCGCCATGA TGGGTGCATT TCAAACGCCC 
GAGGATCGAG TGAAGGCCAT CAACAGACGC GTTACTGTGC TCAAGAAGAA ACTGGTTCAG 
CTGGAGGAAT CCCTGGAGCA GCGACTGGGC TATCGGCCAT CTCAGGCGGA GCGATTGAAC 
GACAAGTACA TGAAGAATGC CCTGGCGGAG TTGAGCAAGC TGCGCAAGGA ACGGCACGAG 
CTCAAGACTG ATCCCATCGC AGCTCTGGGT CTGAAGGTGG GCAGCGGCGG TGGCGCCGGC 
ATCGGTGGCC AGGAGGTCGC CAAGAAGCTG GAGCGCATGA AAGCCACACT CGCTGAAATC 
GAGCAGAATT TGAACGAGAA ACGCGTGAAT GGTCATCGTT CTGAGCAGCT AGAGGATCTG 
AATGCCGACC AACTGGTGCA GGAGAAGAGC GCCGTGCAGC ATGGACTGCT GTACTTTGAG 
AGCCTCTATG GCCATCCCAT TACCAAGGAG GAGCGCGATG CTGCCCGGCC GCTCTACGAT 
CGCTACCGGC AGCTGAAGCG TCTGGTGTCG CGCACCGTGA TGTTTGGAGC GGGCACAGGG 
ATACCCGAGT TGCCCACAAT CCTGGAGCAC GAGGCCATGG TGTTTGAGGC CACTTCCACA 
CCGCTGCAGT ATTCGTCGAA CAACAGCACA GAGACCACCA ACTCGCCCAG CGACTCGAAT 
GCCCCGAATA CTCTGACCCA GAATGCCTCA TCCGACGAGT CGACGACCAC CACCACGTTG 
ACGGGTCCGG CAACGGGAAA TTCGGCCACA AGCCAGGAGA ACCAGGCCGC CAGCGAGAAT 
ATCGCCGCAC TGAGTGTGGA CCAACTCTGG GAGCAGCTAG ATCGAGCACG CGATGAGAAA 
TCCCTGCTAA AATCCACCAT TCGGGAGTAC GAGTCGCTGT TCGAGGAGCA GAATGGACGC 
AAGATGCTCA AGCAGGATCG TCGCTCACTG GAGACGGAAA CCTATGCCCA GTACAAGGAG 
AAGAAGGCCA AGGTCAGACT ACTGCAGGCC CTGATCAAGA AGCATATTGG GCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG6424 ( NM_166246.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725709.1 Drosophila melanogaster uncharacterized protein, transcript variant A (CG6424), mRNA.

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CloneID	OFa10372
Clone ID Related Accession (Same CDS sequence)	NM_137405.4 , NM_001169710.2 , NM_166246.3 , NM_001299617.1
Accession Version	NM_166246.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166246.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGCCATC CCACAACTGG GCTGAACGAA GACGACCTGA GTCGTCTCAC TGGTTCATCA 
TCGTCGACGG CTTCGTGCGG CAGCGCAGCA TCCTCATCAT CGTCCTCATC CTCGTCGTCC 
TCGAATCCCG CTGACCGACA TGAGGTGGTC TGCAGTCCGG AAAAGCCAAC GCCGACGGGC 
ACGGACAAGA GCCAACTGAG CAGCGGCTTT AACTCCACTT ACCTGCCGGA AATCATACAT 
CCGGCTCCGG AATGGCAGAA GTCATCGCGC AAGCGCAAGG AACGCCTGGA CAGCACCTCG 
GCCTTTGGCC AGGATCGAAA GTTACAGCGC AGCAACAGCG AGGAGCTGCT CACAGAACCA 
GCTGCCATTG TGTCTTCCAC AGCCCAGGCA GCAGCCGAAC TTCTGGAGGC CCAGTGTGAG 
GTTATAAGAC GGGTTTCTTC GGAGGATTTC AAACGCACGG CCAGTTACGC CAACTATCGG 
CAGGAGTTCG AAAGGGAGCA GGAGGAGTGC TCGGAGCTGG GTGAGAACAA TGCCTCGTTG 
GCCAACCTGC CGACAGGAGA AGCAGCCAAA GAGCGTCCTC GTCTGGAGAC AAGCCCCGCT 
CGCCAGGCAC CAAAGGAGGC TAGCGATGAC TCCGAGTGCG AGCACGAAAG GCGACGCAGC 
AGTGAGCGTT TCTGCAAATC CCGCCAGCCG CCGGTGCGCA AGTCTGGCGT GGCCAAGAAA 
GTCGGCGGAG GCAGTGGCTC CAAATATCTG CCATCTCGAC GGGATGAGCA AAGCGCCTAC 
AAGTACGACT TGTCGGCTTT GAAGTACGAA CGCAGAGGTT TCATTAGCAG CAGGAAACAG 
CAGCAGAATC AAACACAAGC AATGCAGTCT GCTGCCGATC ACAACGACAA CAACTTGATA 
GACTTCCATC AGCAGCAGAA GGAGCTGCAG TCGAAGCGGA GTGCCAGTAT TTCCCCCACA 
CCCACCACGA GCTCCTCGGC GGGCAGCGAT GACAGCACGC CCACTTCCAA TGCACCCGCG 
CCCATCAAGG CCAAACCCCA GAGGCAGCAG CAGAGCCTGG ATCTCACCTC CGCGCAGGAG 
TCCCTGCCGT GGGAGCGTGG AGATGAACCG GCACCCATTC TGAGCAGAAG ATACGCCCAC 
TCCCGACCAC ATATCGGTGG CAATCTCGAT GCGGCCGCCA AATTGGCCAA GGTCATGCCA 
TATCCCCAGC AGCTGCCCAA ACAGGCATCC ACCGTGCAGC TGCACTCGGA CATCACCGAC 
ATGGACTGCT GCCTGGAGCC CATGGATGCC GTGCGAGCGT TCAGTTCGCT GGAGAATATG 
CGAACCCGCG ACGTTATGCA ACAAGTGTTG CCCGCCATGA TGGGTGCATT TCAAACGCCC 
GAGGATCGAG TGAAGGCCAT CAACAGACGC GTTACTGTGC TCAAGAAGAA ACTGGTTCAG 
CTGGAGGAAT CCCTGGAGCA GCGACTGGGC TATCGGCCAT CTCAGGCGGA GCGATTGAAC 
GACAAGTACA TGAAGAATGC CCTGGCGGAG TTGAGCAAGC TGCGCAAGGA ACGGCACGAG 
CTCAAGACTG ATCCCATCGC AGCTCTGGGT CTGAAGGTGG GCAGCGGCGG TGGCGCCGGC 
ATCGGTGGCC AGGAGGTCGC CAAGAAGCTG GAGCGCATGA AAGCCACACT CGCTGAAATC 
GAGCAGAATT TGAACGAGAA ACGCGTGAAT GGTCATCGTT CTGAGCAGCT AGAGGATCTG 
AATGCCGACC AACTGGTGCA GGAGAAGAGC GCCGTGCAGC ATGGACTGCT GTACTTTGAG 
AGCCTCTATG GCCATCCCAT TACCAAGGAG GAGCGCGATG CTGCCCGGCC GCTCTACGAT 
CGCTACCGGC AGCTGAAGCG TCTGGTGTCG CGCACCGTGA TGTTTGGAGC GGGCACAGGG 
ATACCCGAGT TGCCCACAAT CCTGGAGCAC GAGGCCATGG TGTTTGAGGC CACTTCCACA 
CCGCTGCAGT ATTCGTCGAA CAACAGCACA GAGACCACCA ACTCGCCCAG CGACTCGAAT 
GCCCCGAATA CTCTGACCCA GAATGCCTCA TCCGACGAGT CGACGACCAC CACCACGTTG 
ACGGGTCCGG CAACGGGAAA TTCGGCCACA AGCCAGGAGA ACCAGGCCGC CAGCGAGAAT 
ATCGCCGCAC TGAGTGTGGA CCAACTCTGG GAGCAGCTAG ATCGAGCACG CGATGAGAAA 
TCCCTGCTAA AATCCACCAT TCGGGAGTAC GAGTCGCTGT TCGAGGAGCA GAATGGACGC 
AAGATGCTCA AGCAGGATCG TCGCTCACTG GAGACGGAAA CCTATGCCCA GTACAAGGAG 
AAGAAGGCCA AGGTCAGACT ACTGCAGGCC CTGATCAAGA AGCATATTGG GCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725709.1
CDS	2224..4620
Translation	MRHPTTGLNEDDLSRLTGSSSSTASCGSAASSSSSSSSSSSNPADRHEVVCSPEKPTPTGTDKSQLSSGFNSTYLPEIIHPAPEWQKSSRKRKERLDSTSAFGQDRKLQRSNSEELLTEPAAIVSSTAQAAAELLEAQCEVIRRVSSEDFKRTASYANYRQEFEREQEECSELGENNASLANLPTGEAAKERPRLETSPARQAPKEASDDSECEHERRRSSERFCKSRQPPVRKSGVAKKVGGGSGSKYLPSRRDEQSAYKYDLSALKYERRGFISSRKQQQNQTQAMQSAADHNDNNLIDFHQQQKELQSKRSASISPTPTTSSSAGSDDSTPTSNAPAPIKAKPQRQQQSLDLTSAQESLPWERGDEPAPILSRRYAHSRPHIGGNLDAAAKLAKVMPYPQQLPKQASTVQLHSDITDMDCCLEPMDAVRAFSSLENMRTRDVMQQVLPAMMGAFQTPEDRVKAINRRVTVLKKKLVQLEESLEQRLGYRPSQAERLNDKYMKNALAELSKLRKERHELKTDPIAALGLKVGSGGGAGIGGQEVAKKLERMKATLAEIEQNLNEKRVNGHRSEQLEDLNADQLVQEKSAVQHGLLYFESLYGHPITKEERDAARPLYDRYRQLKRLVSRTVMFGAGTGIPELPTILEHEAMVFEATSTPLQYSSNNSTETTNSPSDSNAPNTLTQNASSDESTTTTTLTGPATGNSATSQENQAASENIAALSVDQLWEQLDRARDEKSLLKSTIREYESLFEEQNGRKMLKQDRRSLETETYAQYKEKKAKVRLLQALIKKHIGH

Target ORF information:

RefSeq Version	NM_166246.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG6424), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166246.3

ORF Insert Sequence:

ATGCGCCATC CCACAACTGG GCTGAACGAA GACGACCTGA GTCGTCTCAC TGGTTCATCA 
TCGTCGACGG CTTCGTGCGG CAGCGCAGCA TCCTCATCAT CGTCCTCATC CTCGTCGTCC 
TCGAATCCCG CTGACCGACA TGAGGTGGTC TGCAGTCCGG AAAAGCCAAC GCCGACGGGC 
ACGGACAAGA GCCAACTGAG CAGCGGCTTT AACTCCACTT ACCTGCCGGA AATCATACAT 
CCGGCTCCGG AATGGCAGAA GTCATCGCGC AAGCGCAAGG AACGCCTGGA CAGCACCTCG 
GCCTTTGGCC AGGATCGAAA GTTACAGCGC AGCAACAGCG AGGAGCTGCT CACAGAACCA 
GCTGCCATTG TGTCTTCCAC AGCCCAGGCA GCAGCCGAAC TTCTGGAGGC CCAGTGTGAG 
GTTATAAGAC GGGTTTCTTC GGAGGATTTC AAACGCACGG CCAGTTACGC CAACTATCGG 
CAGGAGTTCG AAAGGGAGCA GGAGGAGTGC TCGGAGCTGG GTGAGAACAA TGCCTCGTTG 
GCCAACCTGC CGACAGGAGA AGCAGCCAAA GAGCGTCCTC GTCTGGAGAC AAGCCCCGCT 
CGCCAGGCAC CAAAGGAGGC TAGCGATGAC TCCGAGTGCG AGCACGAAAG GCGACGCAGC 
AGTGAGCGTT TCTGCAAATC CCGCCAGCCG CCGGTGCGCA AGTCTGGCGT GGCCAAGAAA 
GTCGGCGGAG GCAGTGGCTC CAAATATCTG CCATCTCGAC GGGATGAGCA AAGCGCCTAC 
AAGTACGACT TGTCGGCTTT GAAGTACGAA CGCAGAGGTT TCATTAGCAG CAGGAAACAG 
CAGCAGAATC AAACACAAGC AATGCAGTCT GCTGCCGATC ACAACGACAA CAACTTGATA 
GACTTCCATC AGCAGCAGAA GGAGCTGCAG TCGAAGCGGA GTGCCAGTAT TTCCCCCACA 
CCCACCACGA GCTCCTCGGC GGGCAGCGAT GACAGCACGC CCACTTCCAA TGCACCCGCG 
CCCATCAAGG CCAAACCCCA GAGGCAGCAG CAGAGCCTGG ATCTCACCTC CGCGCAGGAG 
TCCCTGCCGT GGGAGCGTGG AGATGAACCG GCACCCATTC TGAGCAGAAG ATACGCCCAC 
TCCCGACCAC ATATCGGTGG CAATCTCGAT GCGGCCGCCA AATTGGCCAA GGTCATGCCA 
TATCCCCAGC AGCTGCCCAA ACAGGCATCC ACCGTGCAGC TGCACTCGGA CATCACCGAC 
ATGGACTGCT GCCTGGAGCC CATGGATGCC GTGCGAGCGT TCAGTTCGCT GGAGAATATG 
CGAACCCGCG ACGTTATGCA ACAAGTGTTG CCCGCCATGA TGGGTGCATT TCAAACGCCC 
GAGGATCGAG TGAAGGCCAT CAACAGACGC GTTACTGTGC TCAAGAAGAA ACTGGTTCAG 
CTGGAGGAAT CCCTGGAGCA GCGACTGGGC TATCGGCCAT CTCAGGCGGA GCGATTGAAC 
GACAAGTACA TGAAGAATGC CCTGGCGGAG TTGAGCAAGC TGCGCAAGGA ACGGCACGAG 
CTCAAGACTG ATCCCATCGC AGCTCTGGGT CTGAAGGTGG GCAGCGGCGG TGGCGCCGGC 
ATCGGTGGCC AGGAGGTCGC CAAGAAGCTG GAGCGCATGA AAGCCACACT CGCTGAAATC 
GAGCAGAATT TGAACGAGAA ACGCGTGAAT GGTCATCGTT CTGAGCAGCT AGAGGATCTG 
AATGCCGACC AACTGGTGCA GGAGAAGAGC GCCGTGCAGC ATGGACTGCT GTACTTTGAG 
AGCCTCTATG GCCATCCCAT TACCAAGGAG GAGCGCGATG CTGCCCGGCC GCTCTACGAT 
CGCTACCGGC AGCTGAAGCG TCTGGTGTCG CGCACCGTGA TGTTTGGAGC GGGCACAGGG 
ATACCCGAGT TGCCCACAAT CCTGGAGCAC GAGGCCATGG TGTTTGAGGC CACTTCCACA 
CCGCTGCAGT ATTCGTCGAA CAACAGCACA GAGACCACCA ACTCGCCCAG CGACTCGAAT 
GCCCCGAATA CTCTGACCCA GAATGCCTCA TCCGACGAGT CGACGACCAC CACCACGTTG 
ACGGGTCCGG CAACGGGAAA TTCGGCCACA AGCCAGGAGA ACCAGGCCGC CAGCGAGAAT 
ATCGCCGCAC TGAGTGTGGA CCAACTCTGG GAGCAGCTAG ATCGAGCACG CGATGAGAAA 
TCCCTGCTAA AATCCACCAT TCGGGAGTAC GAGTCGCTGT TCGAGGAGCA GAATGGACGC 
AAGATGCTCA AGCAGGATCG TCGCTCACTG GAGACGGAAA CCTATGCCCA GTACAAGGAG 
AAGAAGGCCA AGGTCAGACT ACTGCAGGCC CTGATCAAGA AGCATATTGG GCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG6424 ( NM_001299617.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286546.1 Drosophila melanogaster uncharacterized protein, transcript variant D (CG6424), mRNA.

Price & Availability↑

CloneID	OFa10372
Clone ID Related Accession (Same CDS sequence)	NM_137405.4 , NM_001169710.2 , NM_166246.3 , NM_001299617.1
Accession Version	NM_001299617.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGCCATC CCACAACTGG GCTGAACGAA GACGACCTGA GTCGTCTCAC TGGTTCATCA 
TCGTCGACGG CTTCGTGCGG CAGCGCAGCA TCCTCATCAT CGTCCTCATC CTCGTCGTCC 
TCGAATCCCG CTGACCGACA TGAGGTGGTC TGCAGTCCGG AAAAGCCAAC GCCGACGGGC 
ACGGACAAGA GCCAACTGAG CAGCGGCTTT AACTCCACTT ACCTGCCGGA AATCATACAT 
CCGGCTCCGG AATGGCAGAA GTCATCGCGC AAGCGCAAGG AACGCCTGGA CAGCACCTCG 
GCCTTTGGCC AGGATCGAAA GTTACAGCGC AGCAACAGCG AGGAGCTGCT CACAGAACCA 
GCTGCCATTG TGTCTTCCAC AGCCCAGGCA GCAGCCGAAC TTCTGGAGGC CCAGTGTGAG 
GTTATAAGAC GGGTTTCTTC GGAGGATTTC AAACGCACGG CCAGTTACGC CAACTATCGG 
CAGGAGTTCG AAAGGGAGCA GGAGGAGTGC TCGGAGCTGG GTGAGAACAA TGCCTCGTTG 
GCCAACCTGC CGACAGGAGA AGCAGCCAAA GAGCGTCCTC GTCTGGAGAC AAGCCCCGCT 
CGCCAGGCAC CAAAGGAGGC TAGCGATGAC TCCGAGTGCG AGCACGAAAG GCGACGCAGC 
AGTGAGCGTT TCTGCAAATC CCGCCAGCCG CCGGTGCGCA AGTCTGGCGT GGCCAAGAAA 
GTCGGCGGAG GCAGTGGCTC CAAATATCTG CCATCTCGAC GGGATGAGCA AAGCGCCTAC 
AAGTACGACT TGTCGGCTTT GAAGTACGAA CGCAGAGGTT TCATTAGCAG CAGGAAACAG 
CAGCAGAATC AAACACAAGC AATGCAGTCT GCTGCCGATC ACAACGACAA CAACTTGATA 
GACTTCCATC AGCAGCAGAA GGAGCTGCAG TCGAAGCGGA GTGCCAGTAT TTCCCCCACA 
CCCACCACGA GCTCCTCGGC GGGCAGCGAT GACAGCACGC CCACTTCCAA TGCACCCGCG 
CCCATCAAGG CCAAACCCCA GAGGCAGCAG CAGAGCCTGG ATCTCACCTC CGCGCAGGAG 
TCCCTGCCGT GGGAGCGTGG AGATGAACCG GCACCCATTC TGAGCAGAAG ATACGCCCAC 
TCCCGACCAC ATATCGGTGG CAATCTCGAT GCGGCCGCCA AATTGGCCAA GGTCATGCCA 
TATCCCCAGC AGCTGCCCAA ACAGGCATCC ACCGTGCAGC TGCACTCGGA CATCACCGAC 
ATGGACTGCT GCCTGGAGCC CATGGATGCC GTGCGAGCGT TCAGTTCGCT GGAGAATATG 
CGAACCCGCG ACGTTATGCA ACAAGTGTTG CCCGCCATGA TGGGTGCATT TCAAACGCCC 
GAGGATCGAG TGAAGGCCAT CAACAGACGC GTTACTGTGC TCAAGAAGAA ACTGGTTCAG 
CTGGAGGAAT CCCTGGAGCA GCGACTGGGC TATCGGCCAT CTCAGGCGGA GCGATTGAAC 
GACAAGTACA TGAAGAATGC CCTGGCGGAG TTGAGCAAGC TGCGCAAGGA ACGGCACGAG 
CTCAAGACTG ATCCCATCGC AGCTCTGGGT CTGAAGGTGG GCAGCGGCGG TGGCGCCGGC 
ATCGGTGGCC AGGAGGTCGC CAAGAAGCTG GAGCGCATGA AAGCCACACT CGCTGAAATC 
GAGCAGAATT TGAACGAGAA ACGCGTGAAT GGTCATCGTT CTGAGCAGCT AGAGGATCTG 
AATGCCGACC AACTGGTGCA GGAGAAGAGC GCCGTGCAGC ATGGACTGCT GTACTTTGAG 
AGCCTCTATG GCCATCCCAT TACCAAGGAG GAGCGCGATG CTGCCCGGCC GCTCTACGAT 
CGCTACCGGC AGCTGAAGCG TCTGGTGTCG CGCACCGTGA TGTTTGGAGC GGGCACAGGG 
ATACCCGAGT TGCCCACAAT CCTGGAGCAC GAGGCCATGG TGTTTGAGGC CACTTCCACA 
CCGCTGCAGT ATTCGTCGAA CAACAGCACA GAGACCACCA ACTCGCCCAG CGACTCGAAT 
GCCCCGAATA CTCTGACCCA GAATGCCTCA TCCGACGAGT CGACGACCAC CACCACGTTG 
ACGGGTCCGG CAACGGGAAA TTCGGCCACA AGCCAGGAGA ACCAGGCCGC CAGCGAGAAT 
ATCGCCGCAC TGAGTGTGGA CCAACTCTGG GAGCAGCTAG ATCGAGCACG CGATGAGAAA 
TCCCTGCTAA AATCCACCAT TCGGGAGTAC GAGTCGCTGT TCGAGGAGCA GAATGGACGC 
AAGATGCTCA AGCAGGATCG TCGCTCACTG GAGACGGAAA CCTATGCCCA GTACAAGGAG 
AAGAAGGCCA AGGTCAGACT ACTGCAGGCC CTGATCAAGA AGCATATTGG GCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286546.1
CDS	1572..3968
Translation	MRHPTTGLNEDDLSRLTGSSSSTASCGSAASSSSSSSSSSSNPADRHEVVCSPEKPTPTGTDKSQLSSGFNSTYLPEIIHPAPEWQKSSRKRKERLDSTSAFGQDRKLQRSNSEELLTEPAAIVSSTAQAAAELLEAQCEVIRRVSSEDFKRTASYANYRQEFEREQEECSELGENNASLANLPTGEAAKERPRLETSPARQAPKEASDDSECEHERRRSSERFCKSRQPPVRKSGVAKKVGGGSGSKYLPSRRDEQSAYKYDLSALKYERRGFISSRKQQQNQTQAMQSAADHNDNNLIDFHQQQKELQSKRSASISPTPTTSSSAGSDDSTPTSNAPAPIKAKPQRQQQSLDLTSAQESLPWERGDEPAPILSRRYAHSRPHIGGNLDAAAKLAKVMPYPQQLPKQASTVQLHSDITDMDCCLEPMDAVRAFSSLENMRTRDVMQQVLPAMMGAFQTPEDRVKAINRRVTVLKKKLVQLEESLEQRLGYRPSQAERLNDKYMKNALAELSKLRKERHELKTDPIAALGLKVGSGGGAGIGGQEVAKKLERMKATLAEIEQNLNEKRVNGHRSEQLEDLNADQLVQEKSAVQHGLLYFESLYGHPITKEERDAARPLYDRYRQLKRLVSRTVMFGAGTGIPELPTILEHEAMVFEATSTPLQYSSNNSTETTNSPSDSNAPNTLTQNASSDESTTTTTLTGPATGNSATSQENQAASENIAALSVDQLWEQLDRARDEKSLLKSTIREYESLFEEQNGRKMLKQDRRSLETETYAQYKEKKAKVRLLQALIKKHIGH

Target ORF information:

RefSeq Version	NM_001299617.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant D (CG6424), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299617.1

ORF Insert Sequence:

ATGCGCCATC CCACAACTGG GCTGAACGAA GACGACCTGA GTCGTCTCAC TGGTTCATCA 
TCGTCGACGG CTTCGTGCGG CAGCGCAGCA TCCTCATCAT CGTCCTCATC CTCGTCGTCC 
TCGAATCCCG CTGACCGACA TGAGGTGGTC TGCAGTCCGG AAAAGCCAAC GCCGACGGGC 
ACGGACAAGA GCCAACTGAG CAGCGGCTTT AACTCCACTT ACCTGCCGGA AATCATACAT 
CCGGCTCCGG AATGGCAGAA GTCATCGCGC AAGCGCAAGG AACGCCTGGA CAGCACCTCG 
GCCTTTGGCC AGGATCGAAA GTTACAGCGC AGCAACAGCG AGGAGCTGCT CACAGAACCA 
GCTGCCATTG TGTCTTCCAC AGCCCAGGCA GCAGCCGAAC TTCTGGAGGC CCAGTGTGAG 
GTTATAAGAC GGGTTTCTTC GGAGGATTTC AAACGCACGG CCAGTTACGC CAACTATCGG 
CAGGAGTTCG AAAGGGAGCA GGAGGAGTGC TCGGAGCTGG GTGAGAACAA TGCCTCGTTG 
GCCAACCTGC CGACAGGAGA AGCAGCCAAA GAGCGTCCTC GTCTGGAGAC AAGCCCCGCT 
CGCCAGGCAC CAAAGGAGGC TAGCGATGAC TCCGAGTGCG AGCACGAAAG GCGACGCAGC 
AGTGAGCGTT TCTGCAAATC CCGCCAGCCG CCGGTGCGCA AGTCTGGCGT GGCCAAGAAA 
GTCGGCGGAG GCAGTGGCTC CAAATATCTG CCATCTCGAC GGGATGAGCA AAGCGCCTAC 
AAGTACGACT TGTCGGCTTT GAAGTACGAA CGCAGAGGTT TCATTAGCAG CAGGAAACAG 
CAGCAGAATC AAACACAAGC AATGCAGTCT GCTGCCGATC ACAACGACAA CAACTTGATA 
GACTTCCATC AGCAGCAGAA GGAGCTGCAG TCGAAGCGGA GTGCCAGTAT TTCCCCCACA 
CCCACCACGA GCTCCTCGGC GGGCAGCGAT GACAGCACGC CCACTTCCAA TGCACCCGCG 
CCCATCAAGG CCAAACCCCA GAGGCAGCAG CAGAGCCTGG ATCTCACCTC CGCGCAGGAG 
TCCCTGCCGT GGGAGCGTGG AGATGAACCG GCACCCATTC TGAGCAGAAG ATACGCCCAC 
TCCCGACCAC ATATCGGTGG CAATCTCGAT GCGGCCGCCA AATTGGCCAA GGTCATGCCA 
TATCCCCAGC AGCTGCCCAA ACAGGCATCC ACCGTGCAGC TGCACTCGGA CATCACCGAC 
ATGGACTGCT GCCTGGAGCC CATGGATGCC GTGCGAGCGT TCAGTTCGCT GGAGAATATG 
CGAACCCGCG ACGTTATGCA ACAAGTGTTG CCCGCCATGA TGGGTGCATT TCAAACGCCC 
GAGGATCGAG TGAAGGCCAT CAACAGACGC GTTACTGTGC TCAAGAAGAA ACTGGTTCAG 
CTGGAGGAAT CCCTGGAGCA GCGACTGGGC TATCGGCCAT CTCAGGCGGA GCGATTGAAC 
GACAAGTACA TGAAGAATGC CCTGGCGGAG TTGAGCAAGC TGCGCAAGGA ACGGCACGAG 
CTCAAGACTG ATCCCATCGC AGCTCTGGGT CTGAAGGTGG GCAGCGGCGG TGGCGCCGGC 
ATCGGTGGCC AGGAGGTCGC CAAGAAGCTG GAGCGCATGA AAGCCACACT CGCTGAAATC 
GAGCAGAATT TGAACGAGAA ACGCGTGAAT GGTCATCGTT CTGAGCAGCT AGAGGATCTG 
AATGCCGACC AACTGGTGCA GGAGAAGAGC GCCGTGCAGC ATGGACTGCT GTACTTTGAG 
AGCCTCTATG GCCATCCCAT TACCAAGGAG GAGCGCGATG CTGCCCGGCC GCTCTACGAT 
CGCTACCGGC AGCTGAAGCG TCTGGTGTCG CGCACCGTGA TGTTTGGAGC GGGCACAGGG 
ATACCCGAGT TGCCCACAAT CCTGGAGCAC GAGGCCATGG TGTTTGAGGC CACTTCCACA 
CCGCTGCAGT ATTCGTCGAA CAACAGCACA GAGACCACCA ACTCGCCCAG CGACTCGAAT 
GCCCCGAATA CTCTGACCCA GAATGCCTCA TCCGACGAGT CGACGACCAC CACCACGTTG 
ACGGGTCCGG CAACGGGAAA TTCGGCCACA AGCCAGGAGA ACCAGGCCGC CAGCGAGAAT 
ATCGCCGCAC TGAGTGTGGA CCAACTCTGG GAGCAGCTAG ATCGAGCACG CGATGAGAAA 
TCCCTGCTAA AATCCACCAT TCGGGAGTAC GAGTCGCTGT TCGAGGAGCA GAATGGACGC 
AAGATGCTCA AGCAGGATCG TCGCTCACTG GAGACGGAAA CCTATGCCCA GTACAAGGAG 
AAGAAGGCCA AGGTCAGACT ACTGCAGGCC CTGATCAAGA AGCATATTGG GCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG6424 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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