eIF3b cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	eIF3b
Entrez Gene ID	36981
Full Name	CG4878 gene product from transcript CG4878-RB
Synonyms	CG4878,Dmel\CG4878,cg4878,eIF3 S9,eIF3-s9,eIF3b
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 54C3-54C3

mRNA and Protein(s)

mRNA	Protein	Name
NM_137384.3	NP_611228.1	eukaryotic translation initiation factor 3 subunit b, isoform B
NM_166239.4	NP_725691.1	eukaryotic translation initiation factor 3 subunit b, isoform A

Pathways from BioSystems

Homologies

Bos taurus (cattle)	EIF3B	NP_001095826.1
Mus musculus (house mouse)	Eif3b	NP_598677.1
Rattus norvegicus (Norway rat)	Eif3b	NP_001026810.1
Magnaporthe oryzae (rice blast fungus)	MGG_01978	XP_003708716.1
Oryza sativa (rice)	Os10g0569200	NP_001065446.1
Gallus gallus (chicken)	EIF3B	XP_003642190.2
Arabidopsis thaliana (thale cress)	EIF3B-2	NP_568477.1
Anopheles gambiae (African malaria mosquito)	EIF3B_ANOGA	XP_320387.3
Eremothecium gossypii	AGOS_ADR399C	NP_984494.1
Arabidopsis thaliana (thale cress)	TIF3B1	NP_568498.1
Pan troglodytes (chimpanzee)	EIF3B	XP_003318263.1
Kluyveromyces lactis	KLLA0A03531g	XP_451155.1
Neurospora crassa	NCU02208	XP_959527.1
Homo sapiens (human)	EIF3B	NP_001032360.1
	EIF3B	XP_001086876.1
Canis lupus familiaris (dog)	EIF3B	XP_536894.3
Danio rerio (zebrafish)	eif3ba	XP_001335480.2
Drosophila melanogaster (fruit fly)	eIF3-S9	NP_725691.1
Saccharomyces cerevisiae (baker's yeast)	PRT1	NP_015006.3
Schizosaccharomyces pombe (fission yeast)	SPAC25G10.08	NP_594528.1
Xenopus tropicalis (tropical clawed frog)	eif3b	NP_001016724.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following eIF3b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eIF3b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13057	NM_166239.4 Latest version!	Drosophila melanogaster eukaryotic translation initiation factor 3 subunit b (eIF3b), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa13057	NM_137384.3 Latest version!	Drosophila melanogaster eukaryotic translation initiation factor 3 subunit b (eIF3b), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

eIF3b ( NM_137384.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611228.1 Drosophila melanogaster eukaryotic translation initiation factor 3 subunit b (eIF3b), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa13057
Clone ID Related Accession (Same CDS sequence)	NM_137384.3 , NM_166239.4
Accession Version	NM_137384.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation initiation factor 3 subunit b, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Dec 17, 2009 this sequence version replaced NM_137384.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCAAGA AGAAAAGCGA GGAGCACTCC GGTACGGATG CGAACGACAG TGATTACCAG 
GAGGAGCCGA ACTTTGACGA TCCGCCCGGG TTCGTGGACA ACATTAGTGA TGAAGATCTG 
CTGGGCGACA TGTTGGCCCA GCGCCCCTCG GAGGCCGATG GCGTCGAGAG TGTGGTGGTA 
GTGGACAATA TCCCAAAGGT GGAGCCGGTG CGCCTGGAGA AGCTGAAGTT GGTCATCAAC 
AAGCTGTTTT CGAACTACGG AGAAATCGTC AATGTGGTCT ATCCCGTCGA CGAGGAGGGC 
AAGACCAAGG GCTACGCCTT CATGGAGTAC AAGCAGGCCA GACAGGCGGA GGAAGCCGTC 
AAGAAGCTCA ACAATCATCG CCTAGACAAA AACCACACCT TTGCCGTCAA TCTCTTCACC 
GATTTCCAAA AGTACGAAAA CATCCCCGAG AAGTGGGAGC CGCCAACCGT GCAGACCTTC 
AAAGTGCAGA GCGATCTATA CAACTTCATC AATGACCCGG ACACTTATGA CCAGTACTGC 
GTGGCAGCGG AGACCGCTCC CAACTGCGTG CAGGTTGGTT TCTGGCAAAA CGTATTGCCC 
GAACCCTTTG AGCTGGAGAC TCGCGAACGC TTCACCGACA CCTTCGTCAA GTGGTCGCCC 
CTGGGTACTT ATGTAGTCAC CTTCCACAAA CCCGGTGTGG CCATCTGGGG TGGCAGTAGC 
TTCCAGAAGA TTCAGAAGTT TCCCCATCCT GGTACCCAAT TCGTTGAGTT TTCGCCCTGC 
GAGAATTACC TAGTGACTTA CGGACCGACA CCCACGGGCC AAAAGATCAT CATCTGGGAC 
ATTCGCACTG GAGCCGAGAA ACGTTCGTTT GTTGCCGATG GCATGTCGGT TCTTTCTATG 
TTCCGCTGGT CGCACGACGA CAAGTTTGTG GCCCGCATGG GCGAGAATTC GATCCACATC 
TACGAGACAC CCTCTTTCTA TCTTCTGGAC CTGAAGTCCA TTAAGATTCC CGGCATTCGT 
GGCTTCTCTT GGTCCCCCAC AGACAACGTG ATTGCCTACT GGGTGGAGGA GCAGAACCAA 
ATACCCGCCC GCGTTACCCT GATGGAGATC CCCAAGAAGC GTGAGATCCG TAACAAGAAC 
CTTTTCCATG TGGCCGATTG CAAGTTACAT TGGCAAAAGT CCGGCGACTA TCTGTGCGTT 
AAGGTAGATC GTTACTCAAA GTTGAAGAAG GATAAGAAGG ACCTGGACGT CAAGTTTCTG 
GGCATGTTCT ACAACTTTGA GATCTTCCAC ATGCGCGAGA AGGAGATTCC CGTCGACTCT 
GTGGAGATTC GTGAGCTTAT TTTGGCCTTT GCCTGGGAGC CCGTCGGCAA CAAGTTCTCC 
ATCATTCACG GCGAGACCAA CTCGTCGAAT GTGAGCTTCT ACGAGGTGAA CAAGGGCGTT 
AAGCCCAGCT TGGTGAAGCG TCTAGAAAAG AAGTCATGCA CACATCTGTT CTGGTCGCCA 
CGCGGTCAGT TTATCGTAAT GGCCAACCTC ACCATGGGTA CCTTCGAGTT TGTGGACTCC 
ACCAACGATT ACATCATCAC GGCATCGCCC GATCATTTCC GCGCTTCCGA GGTCGAGTGG 
GATCCCACTG GACGCTACGT TGTGACTGGT GTCTCGTCAT GGAAGGTGAA AGAGGATACC 
GGCTTCAACA TGTACACGTT CCAGGGCCGC ATCATCAAGC GTACCATTCT TAAGAACTTT 
GTGCAGTTCT TGTGGCGCCC GCGTCCTCCC ACCCTGCTTA GCGAGGAGAA GCAGAAGGAG 
ATCAAGAAGA ACTTGAAGAA GTACTACGCT GCCTTCGAAC AAAAGGATCG CCTGCGCCTT 
ACCCGTGCCT CCAAGGAGCT GCTCGAGAAG CGCTCGCAGC TGCGCGAGAC CTTTATGGAG 
TACCGCAACA AGCGTATTGC CGAGTGGGCG GAGCAGAAGA GTCGTCGCAT CATGCTGCGA 
GGGCATGTGG ACACAGACAA CCTGGAAACT GACGAAGTGG ATGAAGAAAT TGTTGAATTT 
TTAGTCAAGG AAGAAGTCAC TCTGCTGGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611228.1
CDS	128..2200
Translation	MAKKKSEEHSGTDANDSDYQEEPNFDDPPGFVDNISDEDLLGDMLAQRPSEADGVESVVVVDNIPKVEPVRLEKLKLVINKLFSNYGEIVNVVYPVDEEGKTKGYAFMEYKQARQAEEAVKKLNNHRLDKNHTFAVNLFTDFQKYENIPEKWEPPTVQTFKVQSDLYNFINDPDTYDQYCVAAETAPNCVQVGFWQNVLPEPFELETRERFTDTFVKWSPLGTYVVTFHKPGVAIWGGSSFQKIQKFPHPGTQFVEFSPCENYLVTYGPTPTGQKIIIWDIRTGAEKRSFVADGMSVLSMFRWSHDDKFVARMGENSIHIYETPSFYLLDLKSIKIPGIRGFSWSPTDNVIAYWVEEQNQIPARVTLMEIPKKREIRNKNLFHVADCKLHWQKSGDYLCVKVDRYSKLKKDKKDLDVKFLGMFYNFEIFHMREKEIPVDSVEIRELILAFAWEPVGNKFSIIHGETNSSNVSFYEVNKGVKPSLVKRLEKKSCTHLFWSPRGQFIVMANLTMGTFEFVDSTNDYIITASPDHFRASEVEWDPTGRYVVTGVSSWKVKEDTGFNMYTFQGRIIKRTILKNFVQFLWRPRPPTLLSEEKQKEIKKNLKKYYAAFEQKDRLRLTRASKELLEKRSQLRETFMEYRNKRIAEWAEQKSRRIMLRGHVDTDNLETDEVDEEIVEFLVKEEVTLLE

Target ORF information:

RefSeq Version	NM_137384.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation initiation factor 3 subunit b (eIF3b), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137384.3

ORF Insert Sequence:

ATGGCCAAGA AGAAAAGCGA GGAGCACTCC GGTACGGATG CGAACGACAG TGATTACCAG 
GAGGAGCCGA ACTTTGACGA TCCGCCCGGG TTCGTGGACA ACATTAGTGA TGAAGATCTG 
CTGGGCGACA TGTTGGCCCA GCGCCCCTCG GAGGCCGATG GCGTCGAGAG TGTGGTGGTA 
GTGGACAATA TCCCAAAGGT GGAGCCGGTG CGCCTGGAGA AGCTGAAGTT GGTCATCAAC 
AAGCTGTTTT CGAACTACGG AGAAATCGTC AATGTGGTCT ATCCCGTCGA CGAGGAGGGC 
AAGACCAAGG GCTACGCCTT CATGGAGTAC AAGCAGGCCA GACAGGCGGA GGAAGCCGTC 
AAGAAGCTCA ACAATCATCG CCTAGACAAA AACCACACCT TTGCCGTCAA TCTCTTCACC 
GATTTCCAAA AGTACGAAAA CATCCCCGAG AAGTGGGAGC CGCCAACCGT GCAGACCTTC 
AAAGTGCAGA GCGATCTATA CAACTTCATC AATGACCCGG ACACTTATGA CCAGTACTGC 
GTGGCAGCGG AGACCGCTCC CAACTGCGTG CAGGTTGGTT TCTGGCAAAA CGTATTGCCC 
GAACCCTTTG AGCTGGAGAC TCGCGAACGC TTCACCGACA CCTTCGTCAA GTGGTCGCCC 
CTGGGTACTT ATGTAGTCAC CTTCCACAAA CCCGGTGTGG CCATCTGGGG TGGCAGTAGC 
TTCCAGAAGA TTCAGAAGTT TCCCCATCCT GGTACCCAAT TCGTTGAGTT TTCGCCCTGC 
GAGAATTACC TAGTGACTTA CGGACCGACA CCCACGGGCC AAAAGATCAT CATCTGGGAC 
ATTCGCACTG GAGCCGAGAA ACGTTCGTTT GTTGCCGATG GCATGTCGGT TCTTTCTATG 
TTCCGCTGGT CGCACGACGA CAAGTTTGTG GCCCGCATGG GCGAGAATTC GATCCACATC 
TACGAGACAC CCTCTTTCTA TCTTCTGGAC CTGAAGTCCA TTAAGATTCC CGGCATTCGT 
GGCTTCTCTT GGTCCCCCAC AGACAACGTG ATTGCCTACT GGGTGGAGGA GCAGAACCAA 
ATACCCGCCC GCGTTACCCT GATGGAGATC CCCAAGAAGC GTGAGATCCG TAACAAGAAC 
CTTTTCCATG TGGCCGATTG CAAGTTACAT TGGCAAAAGT CCGGCGACTA TCTGTGCGTT 
AAGGTAGATC GTTACTCAAA GTTGAAGAAG GATAAGAAGG ACCTGGACGT CAAGTTTCTG 
GGCATGTTCT ACAACTTTGA GATCTTCCAC ATGCGCGAGA AGGAGATTCC CGTCGACTCT 
GTGGAGATTC GTGAGCTTAT TTTGGCCTTT GCCTGGGAGC CCGTCGGCAA CAAGTTCTCC 
ATCATTCACG GCGAGACCAA CTCGTCGAAT GTGAGCTTCT ACGAGGTGAA CAAGGGCGTT 
AAGCCCAGCT TGGTGAAGCG TCTAGAAAAG AAGTCATGCA CACATCTGTT CTGGTCGCCA 
CGCGGTCAGT TTATCGTAAT GGCCAACCTC ACCATGGGTA CCTTCGAGTT TGTGGACTCC 
ACCAACGATT ACATCATCAC GGCATCGCCC GATCATTTCC GCGCTTCCGA GGTCGAGTGG 
GATCCCACTG GACGCTACGT TGTGACTGGT GTCTCGTCAT GGAAGGTGAA AGAGGATACC 
GGCTTCAACA TGTACACGTT CCAGGGCCGC ATCATCAAGC GTACCATTCT TAAGAACTTT 
GTGCAGTTCT TGTGGCGCCC GCGTCCTCCC ACCCTGCTTA GCGAGGAGAA GCAGAAGGAG 
ATCAAGAAGA ACTTGAAGAA GTACTACGCT GCCTTCGAAC AAAAGGATCG CCTGCGCCTT 
ACCCGTGCCT CCAAGGAGCT GCTCGAGAAG CGCTCGCAGC TGCGCGAGAC CTTTATGGAG 
TACCGCAACA AGCGTATTGC CGAGTGGGCG GAGCAGAAGA GTCGTCGCAT CATGCTGCGA 
GGGCATGTGG ACACAGACAA CCTGGAAACT GACGAAGTGG ATGAAGAAAT TGTTGAATTT 
TTAGTCAAGG AAGAAGTCAC TCTGCTGGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eIF3b ( NM_166239.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725691.1 Drosophila melanogaster eukaryotic translation initiation factor 3 subunit b (eIF3b), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa13057
Clone ID Related Accession (Same CDS sequence)	NM_137384.3 , NM_166239.4
Accession Version	NM_166239.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation initiation factor 3 subunit b, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166239.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCAAGA AGAAAAGCGA GGAGCACTCC GGTACGGATG CGAACGACAG TGATTACCAG 
GAGGAGCCGA ACTTTGACGA TCCGCCCGGG TTCGTGGACA ACATTAGTGA TGAAGATCTG 
CTGGGCGACA TGTTGGCCCA GCGCCCCTCG GAGGCCGATG GCGTCGAGAG TGTGGTGGTA 
GTGGACAATA TCCCAAAGGT GGAGCCGGTG CGCCTGGAGA AGCTGAAGTT GGTCATCAAC 
AAGCTGTTTT CGAACTACGG AGAAATCGTC AATGTGGTCT ATCCCGTCGA CGAGGAGGGC 
AAGACCAAGG GCTACGCCTT CATGGAGTAC AAGCAGGCCA GACAGGCGGA GGAAGCCGTC 
AAGAAGCTCA ACAATCATCG CCTAGACAAA AACCACACCT TTGCCGTCAA TCTCTTCACC 
GATTTCCAAA AGTACGAAAA CATCCCCGAG AAGTGGGAGC CGCCAACCGT GCAGACCTTC 
AAAGTGCAGA GCGATCTATA CAACTTCATC AATGACCCGG ACACTTATGA CCAGTACTGC 
GTGGCAGCGG AGACCGCTCC CAACTGCGTG CAGGTTGGTT TCTGGCAAAA CGTATTGCCC 
GAACCCTTTG AGCTGGAGAC TCGCGAACGC TTCACCGACA CCTTCGTCAA GTGGTCGCCC 
CTGGGTACTT ATGTAGTCAC CTTCCACAAA CCCGGTGTGG CCATCTGGGG TGGCAGTAGC 
TTCCAGAAGA TTCAGAAGTT TCCCCATCCT GGTACCCAAT TCGTTGAGTT TTCGCCCTGC 
GAGAATTACC TAGTGACTTA CGGACCGACA CCCACGGGCC AAAAGATCAT CATCTGGGAC 
ATTCGCACTG GAGCCGAGAA ACGTTCGTTT GTTGCCGATG GCATGTCGGT TCTTTCTATG 
TTCCGCTGGT CGCACGACGA CAAGTTTGTG GCCCGCATGG GCGAGAATTC GATCCACATC 
TACGAGACAC CCTCTTTCTA TCTTCTGGAC CTGAAGTCCA TTAAGATTCC CGGCATTCGT 
GGCTTCTCTT GGTCCCCCAC AGACAACGTG ATTGCCTACT GGGTGGAGGA GCAGAACCAA 
ATACCCGCCC GCGTTACCCT GATGGAGATC CCCAAGAAGC GTGAGATCCG TAACAAGAAC 
CTTTTCCATG TGGCCGATTG CAAGTTACAT TGGCAAAAGT CCGGCGACTA TCTGTGCGTT 
AAGGTAGATC GTTACTCAAA GTTGAAGAAG GATAAGAAGG ACCTGGACGT CAAGTTTCTG 
GGCATGTTCT ACAACTTTGA GATCTTCCAC ATGCGCGAGA AGGAGATTCC CGTCGACTCT 
GTGGAGATTC GTGAGCTTAT TTTGGCCTTT GCCTGGGAGC CCGTCGGCAA CAAGTTCTCC 
ATCATTCACG GCGAGACCAA CTCGTCGAAT GTGAGCTTCT ACGAGGTGAA CAAGGGCGTT 
AAGCCCAGCT TGGTGAAGCG TCTAGAAAAG AAGTCATGCA CACATCTGTT CTGGTCGCCA 
CGCGGTCAGT TTATCGTAAT GGCCAACCTC ACCATGGGTA CCTTCGAGTT TGTGGACTCC 
ACCAACGATT ACATCATCAC GGCATCGCCC GATCATTTCC GCGCTTCCGA GGTCGAGTGG 
GATCCCACTG GACGCTACGT TGTGACTGGT GTCTCGTCAT GGAAGGTGAA AGAGGATACC 
GGCTTCAACA TGTACACGTT CCAGGGCCGC ATCATCAAGC GTACCATTCT TAAGAACTTT 
GTGCAGTTCT TGTGGCGCCC GCGTCCTCCC ACCCTGCTTA GCGAGGAGAA GCAGAAGGAG 
ATCAAGAAGA ACTTGAAGAA GTACTACGCT GCCTTCGAAC AAAAGGATCG CCTGCGCCTT 
ACCCGTGCCT CCAAGGAGCT GCTCGAGAAG CGCTCGCAGC TGCGCGAGAC CTTTATGGAG 
TACCGCAACA AGCGTATTGC CGAGTGGGCG GAGCAGAAGA GTCGTCGCAT CATGCTGCGA 
GGGCATGTGG ACACAGACAA CCTGGAAACT GACGAAGTGG ATGAAGAAAT TGTTGAATTT 
TTAGTCAAGG AAGAAGTCAC TCTGCTGGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725691.1
CDS	133..2205
Translation	MAKKKSEEHSGTDANDSDYQEEPNFDDPPGFVDNISDEDLLGDMLAQRPSEADGVESVVVVDNIPKVEPVRLEKLKLVINKLFSNYGEIVNVVYPVDEEGKTKGYAFMEYKQARQAEEAVKKLNNHRLDKNHTFAVNLFTDFQKYENIPEKWEPPTVQTFKVQSDLYNFINDPDTYDQYCVAAETAPNCVQVGFWQNVLPEPFELETRERFTDTFVKWSPLGTYVVTFHKPGVAIWGGSSFQKIQKFPHPGTQFVEFSPCENYLVTYGPTPTGQKIIIWDIRTGAEKRSFVADGMSVLSMFRWSHDDKFVARMGENSIHIYETPSFYLLDLKSIKIPGIRGFSWSPTDNVIAYWVEEQNQIPARVTLMEIPKKREIRNKNLFHVADCKLHWQKSGDYLCVKVDRYSKLKKDKKDLDVKFLGMFYNFEIFHMREKEIPVDSVEIRELILAFAWEPVGNKFSIIHGETNSSNVSFYEVNKGVKPSLVKRLEKKSCTHLFWSPRGQFIVMANLTMGTFEFVDSTNDYIITASPDHFRASEVEWDPTGRYVVTGVSSWKVKEDTGFNMYTFQGRIIKRTILKNFVQFLWRPRPPTLLSEEKQKEIKKNLKKYYAAFEQKDRLRLTRASKELLEKRSQLRETFMEYRNKRIAEWAEQKSRRIMLRGHVDTDNLETDEVDEEIVEFLVKEEVTLLE

Target ORF information:

RefSeq Version	NM_166239.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation initiation factor 3 subunit b (eIF3b), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166239.4

ORF Insert Sequence:

ATGGCCAAGA AGAAAAGCGA GGAGCACTCC GGTACGGATG CGAACGACAG TGATTACCAG 
GAGGAGCCGA ACTTTGACGA TCCGCCCGGG TTCGTGGACA ACATTAGTGA TGAAGATCTG 
CTGGGCGACA TGTTGGCCCA GCGCCCCTCG GAGGCCGATG GCGTCGAGAG TGTGGTGGTA 
GTGGACAATA TCCCAAAGGT GGAGCCGGTG CGCCTGGAGA AGCTGAAGTT GGTCATCAAC 
AAGCTGTTTT CGAACTACGG AGAAATCGTC AATGTGGTCT ATCCCGTCGA CGAGGAGGGC 
AAGACCAAGG GCTACGCCTT CATGGAGTAC AAGCAGGCCA GACAGGCGGA GGAAGCCGTC 
AAGAAGCTCA ACAATCATCG CCTAGACAAA AACCACACCT TTGCCGTCAA TCTCTTCACC 
GATTTCCAAA AGTACGAAAA CATCCCCGAG AAGTGGGAGC CGCCAACCGT GCAGACCTTC 
AAAGTGCAGA GCGATCTATA CAACTTCATC AATGACCCGG ACACTTATGA CCAGTACTGC 
GTGGCAGCGG AGACCGCTCC CAACTGCGTG CAGGTTGGTT TCTGGCAAAA CGTATTGCCC 
GAACCCTTTG AGCTGGAGAC TCGCGAACGC TTCACCGACA CCTTCGTCAA GTGGTCGCCC 
CTGGGTACTT ATGTAGTCAC CTTCCACAAA CCCGGTGTGG CCATCTGGGG TGGCAGTAGC 
TTCCAGAAGA TTCAGAAGTT TCCCCATCCT GGTACCCAAT TCGTTGAGTT TTCGCCCTGC 
GAGAATTACC TAGTGACTTA CGGACCGACA CCCACGGGCC AAAAGATCAT CATCTGGGAC 
ATTCGCACTG GAGCCGAGAA ACGTTCGTTT GTTGCCGATG GCATGTCGGT TCTTTCTATG 
TTCCGCTGGT CGCACGACGA CAAGTTTGTG GCCCGCATGG GCGAGAATTC GATCCACATC 
TACGAGACAC CCTCTTTCTA TCTTCTGGAC CTGAAGTCCA TTAAGATTCC CGGCATTCGT 
GGCTTCTCTT GGTCCCCCAC AGACAACGTG ATTGCCTACT GGGTGGAGGA GCAGAACCAA 
ATACCCGCCC GCGTTACCCT GATGGAGATC CCCAAGAAGC GTGAGATCCG TAACAAGAAC 
CTTTTCCATG TGGCCGATTG CAAGTTACAT TGGCAAAAGT CCGGCGACTA TCTGTGCGTT 
AAGGTAGATC GTTACTCAAA GTTGAAGAAG GATAAGAAGG ACCTGGACGT CAAGTTTCTG 
GGCATGTTCT ACAACTTTGA GATCTTCCAC ATGCGCGAGA AGGAGATTCC CGTCGACTCT 
GTGGAGATTC GTGAGCTTAT TTTGGCCTTT GCCTGGGAGC CCGTCGGCAA CAAGTTCTCC 
ATCATTCACG GCGAGACCAA CTCGTCGAAT GTGAGCTTCT ACGAGGTGAA CAAGGGCGTT 
AAGCCCAGCT TGGTGAAGCG TCTAGAAAAG AAGTCATGCA CACATCTGTT CTGGTCGCCA 
CGCGGTCAGT TTATCGTAAT GGCCAACCTC ACCATGGGTA CCTTCGAGTT TGTGGACTCC 
ACCAACGATT ACATCATCAC GGCATCGCCC GATCATTTCC GCGCTTCCGA GGTCGAGTGG 
GATCCCACTG GACGCTACGT TGTGACTGGT GTCTCGTCAT GGAAGGTGAA AGAGGATACC 
GGCTTCAACA TGTACACGTT CCAGGGCCGC ATCATCAAGC GTACCATTCT TAAGAACTTT 
GTGCAGTTCT TGTGGCGCCC GCGTCCTCCC ACCCTGCTTA GCGAGGAGAA GCAGAAGGAG 
ATCAAGAAGA ACTTGAAGAA GTACTACGCT GCCTTCGAAC AAAAGGATCG CCTGCGCCTT 
ACCCGTGCCT CCAAGGAGCT GCTCGAGAAG CGCTCGCAGC TGCGCGAGAC CTTTATGGAG 
TACCGCAACA AGCGTATTGC CGAGTGGGCG GAGCAGAAGA GTCGTCGCAT CATGCTGCGA 
GGGCATGTGG ACACAGACAA CCTGGAAACT GACGAAGTGG ATGAAGAAAT TGTTGAATTT 
TTAGTCAAGG AAGAAGTCAC TCTGCTGGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
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The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
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Combined evidence annotation of transposable elements in genome sequences.
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eIF3b cDNA ORF clone, Drosophila melanogaster(fruit fly)

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ORF Insert Sequence:

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