tef cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	tef
Entrez Gene ID	36920
Full Name	teflon
Synonyms	CG8961,Dmel\CG8961,l(2)k15914
General protein information	Preferred Names teflon Names CG8961 gene product from transcript CG8961-RA CG8961-PA lethal (2) k15914 tef-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 53F1-53F2\|2-80.0 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_079042.4	NP_523766.1	teflon

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A role for Tef as a bridging molecule that holds autosome bivalents together via heterochromatic connections.
Title: Molecular characterization of teflon, a gene required for meiotic autosome segregation in male Drosophila melanogaster.

The following tef gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tef cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10311	NM_079042.4 Latest version!	Drosophila melanogaster teflon (tef), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tef ( NM_079042.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523766.1 Drosophila melanogaster teflon (tef), mRNA.

Price & Availability↑

CloneID	OFa10311
Clone ID Related Accession (Same CDS sequence)	NM_079042.4
Accession Version	NM_079042.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	teflon
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_079042.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTAAGT TTCTGGACAT GCTATCGGGG AGCCAGTGCG TCAGCCTGGA AAAGTGCGGC 
GATGTGGTGG TCTCAACCAA CGACTGTATG ATTGCACTTT ACTGCCACTT CTGCCGTGAC 
CTCTTCACAC AACTGCCGGA GTTCTTAAGA CACCTCCAAA GCAACCATTC GGATGTGCTG 
CACTTCACCA AGGAGCACAA CGTTTACAGC GTGGAGGAGC TGTTGTCCGG TGAGCAGGGT 
AAAGCCCACG AGGACGCCCA GTCGGCGGGG CACAACAGCA GCAGCGGTGA CTCTAGTTCA 
CTGATGAATT CAGAGGATTC TAGGGCCATC GAAGGCTCAG AAGATAACTC AGACAATAGC 
CCAATGAAAC CTGAACAAAT AGGGAAACAG AGTGAAATAA ACCTGCTAGC GGAAGTGACA 
AACATTTTAT TGCAAACCAA CGACAAGGAA TTAAAACCCG AAAATGGCGT ATTCAACAGA 
CCACGCAAAA AGGCTAATAA CGAGAGCAGT TCTTTGAAGA TATGCGATCT GAAGAGTCAC 
ACTATTGCAA GGACTTCGCG AAAGCGAATG AGCATGGTAA AGAACCGCAT CCTGCGAGTC 
CTTGACAGCG ATTTGTCTGC AAAGCTTGAA ATTAAACCTC CAGAGCCCAA CTCCAAACTT 
TCTATAACTG AGCCCATCCA AGAAGACAAC ATACCTGGAA CATGTTTTGA CACTCCTACA 
AAGCCCATAC CGAGCTCATC TCAATTAAGT GTAAGAAAGT CTTCTTTAAC AGAGGCAAAC 
CAAATATGTA CCAAATATGC CGAGAAGAAA ACGGCGCCTA CCATGCCTAA GCTACTGAAC 
TGTGTACCAA AGCCCATATT AACTAGTCAA CAAGCTCATA CTAATGCCGA GATAAGTGAG 
ATAAATGAAA CCTATCACCT AGCAGCTAGT CAAGTGACAA AAACCACGAA AACATTTCCC 
GTAAAAATAA CACAAATAGA TATTTTACAA CCAGTTAAGT TACCTAAAAC TTTAATAACA 
CCAATCAATG AAGAGGGTGT CAGTGACCAA GTGGAGAATA GCACGAATAA TATAAACAAT 
GCCCAAAGTC TGCCGAAAGA AAACACAAAA AAGTTTTTTA AAAAGCGTAG CGAATTAGGG 
ATAAAAGCTC AAGGCAGTCC CAACAAATTT TTTAAAATAA TAAAGTCCAA GGCAAACCCC 
ATTATTGTAA AACGGGTGCA GACCACCAGT GCCAAAGCTA GTACAAACAA AATCCAAATA 
CGATCTAATG ACAAAACTAA TTGTTTTGCT TCTGAGTTTA ACAGCACAAA GATCCGCAAG 
TTAAAGATGG AAAATTGCGT CGACCTAAAA TCGGAAGATC CCTGCGCCAA TACAAATACG 
CGTTTAAACA AATTGGCAAC AATAAGCAGT TGCGAAATAT TAAAAGCGGT TGGATTGCCG 
GCAATCACAG ATAACCCGAT CGAGGATACA TTATTGCCAG ACGAGTTAGA AACGATCCGA 
AAAAAGGCAG ATCAATTCAT CAAAATCTAC AGGAAGTACG ATTCTATTTG GAATTATAGA 
AAAATTTGTC CGCCAGCGAA ACCGGACTTT ATTTCTCAAC AGATATTTGC TTTAACCAGA 
GAAGTTAACA AGACAATGTT ATGCAACCTG GCGAACAGCG ATATAAAAGG CATTATTAAT 
CAGATTTCAG TTTGGCATTA CAATATATAT ACCCAATATA TTGACTTGGA TACCATTTCG 
GAAAATGCCC GATATACTTT AAAGCTATTC TCGTTTTTAC CTGTTAGTTT TGCTTACTTT 
TGCAAATGTT GCGACGATAT TTTCATCTTA AAGAAAGAGT ACTTAAAGCA TTTGATTTCT 
CACCAAGTTC GGTTTCAGTG CACAAAATGC ATTAAAGTAT TCAAGTACAA GGGATACTAT 
GAAAAGCATT TGCGAAACGC TCATTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523766.1
CDS	104..2053
Translation	MSKFLDMLSGSQCVSLEKCGDVVVSTNDCMIALYCHFCRDLFTQLPEFLRHLQSNHSDVLHFTKEHNVYSVEELLSGEQGKAHEDAQSAGHNSSSGDSSSLMNSEDSRAIEGSEDNSDNSPMKPEQIGKQSEINLLAEVTNILLQTNDKELKPENGVFNRPRKKANNESSSLKICDLKSHTIARTSRKRMSMVKNRILRVLDSDLSAKLEIKPPEPNSKLSITEPIQEDNIPGTCFDTPTKPIPSSSQLSVRKSSLTEANQICTKYAEKKTAPTMPKLLNCVPKPILTSQQAHTNAEISEINETYHLAASQVTKTTKTFPVKITQIDILQPVKLPKTLITPINEEGVSDQVENSTNNINNAQSLPKENTKKFFKKRSELGIKAQGSPNKFFKIIKSKANPIIVKRVQTTSAKASTNKIQIRSNDKTNCFASEFNSTKIRKLKMENCVDLKSEDPCANTNTRLNKLATISSCEILKAVGLPAITDNPIEDTLLPDELETIRKKADQFIKIYRKYDSIWNYRKICPPAKPDFISQQIFALTREVNKTMLCNLANSDIKGIINQISVWHYNIYTQYIDLDTISENARYTLKLFSFLPVSFAYFCKCCDDIFILKKEYLKHLISHQVRFQCTKCIKVFKYKGYYEKHLRNAHS

Target ORF information:

RefSeq Version	NM_079042.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster teflon (tef), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079042.4

ORF Insert Sequence:

ATGTCTAAGT TTCTGGACAT GCTATCGGGG AGCCAGTGCG TCAGCCTGGA AAAGTGCGGC 
GATGTGGTGG TCTCAACCAA CGACTGTATG ATTGCACTTT ACTGCCACTT CTGCCGTGAC 
CTCTTCACAC AACTGCCGGA GTTCTTAAGA CACCTCCAAA GCAACCATTC GGATGTGCTG 
CACTTCACCA AGGAGCACAA CGTTTACAGC GTGGAGGAGC TGTTGTCCGG TGAGCAGGGT 
AAAGCCCACG AGGACGCCCA GTCGGCGGGG CACAACAGCA GCAGCGGTGA CTCTAGTTCA 
CTGATGAATT CAGAGGATTC TAGGGCCATC GAAGGCTCAG AAGATAACTC AGACAATAGC 
CCAATGAAAC CTGAACAAAT AGGGAAACAG AGTGAAATAA ACCTGCTAGC GGAAGTGACA 
AACATTTTAT TGCAAACCAA CGACAAGGAA TTAAAACCCG AAAATGGCGT ATTCAACAGA 
CCACGCAAAA AGGCTAATAA CGAGAGCAGT TCTTTGAAGA TATGCGATCT GAAGAGTCAC 
ACTATTGCAA GGACTTCGCG AAAGCGAATG AGCATGGTAA AGAACCGCAT CCTGCGAGTC 
CTTGACAGCG ATTTGTCTGC AAAGCTTGAA ATTAAACCTC CAGAGCCCAA CTCCAAACTT 
TCTATAACTG AGCCCATCCA AGAAGACAAC ATACCTGGAA CATGTTTTGA CACTCCTACA 
AAGCCCATAC CGAGCTCATC TCAATTAAGT GTAAGAAAGT CTTCTTTAAC AGAGGCAAAC 
CAAATATGTA CCAAATATGC CGAGAAGAAA ACGGCGCCTA CCATGCCTAA GCTACTGAAC 
TGTGTACCAA AGCCCATATT AACTAGTCAA CAAGCTCATA CTAATGCCGA GATAAGTGAG 
ATAAATGAAA CCTATCACCT AGCAGCTAGT CAAGTGACAA AAACCACGAA AACATTTCCC 
GTAAAAATAA CACAAATAGA TATTTTACAA CCAGTTAAGT TACCTAAAAC TTTAATAACA 
CCAATCAATG AAGAGGGTGT CAGTGACCAA GTGGAGAATA GCACGAATAA TATAAACAAT 
GCCCAAAGTC TGCCGAAAGA AAACACAAAA AAGTTTTTTA AAAAGCGTAG CGAATTAGGG 
ATAAAAGCTC AAGGCAGTCC CAACAAATTT TTTAAAATAA TAAAGTCCAA GGCAAACCCC 
ATTATTGTAA AACGGGTGCA GACCACCAGT GCCAAAGCTA GTACAAACAA AATCCAAATA 
CGATCTAATG ACAAAACTAA TTGTTTTGCT TCTGAGTTTA ACAGCACAAA GATCCGCAAG 
TTAAAGATGG AAAATTGCGT CGACCTAAAA TCGGAAGATC CCTGCGCCAA TACAAATACG 
CGTTTAAACA AATTGGCAAC AATAAGCAGT TGCGAAATAT TAAAAGCGGT TGGATTGCCG 
GCAATCACAG ATAACCCGAT CGAGGATACA TTATTGCCAG ACGAGTTAGA AACGATCCGA 
AAAAAGGCAG ATCAATTCAT CAAAATCTAC AGGAAGTACG ATTCTATTTG GAATTATAGA 
AAAATTTGTC CGCCAGCGAA ACCGGACTTT ATTTCTCAAC AGATATTTGC TTTAACCAGA 
GAAGTTAACA AGACAATGTT ATGCAACCTG GCGAACAGCG ATATAAAAGG CATTATTAAT 
CAGATTTCAG TTTGGCATTA CAATATATAT ACCCAATATA TTGACTTGGA TACCATTTCG 
GAAAATGCCC GATATACTTT AAAGCTATTC TCGTTTTTAC CTGTTAGTTT TGCTTACTTT 
TGCAAATGTT GCGACGATAT TTTCATCTTA AAGAAAGAGT ACTTAAAGCA TTTGATTTCT 
CACCAAGTTC GGTTTCAGTG CACAAAATGC ATTAAAGTAT TCAAGTACAA GGGATACTAT 
GAAAAGCATT TGCGAAACGC TCATTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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tef cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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