Asph cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Asph
Entrez Gene ID	36778
Full Name	Aspartyl beta-hydroxylase
Synonyms	BAH/humbug,CG18658,CG8421,Dmel\CG8421
General protein information	Preferred Names Aspartyl beta-hydroxylase Names CG8421 gene product from transcript CG8421-RL Asph-PA Asph-PB Asph-PG Asph-PH Asph-PI Asph-PJ Asph-PK Asph-PL CG8421-PA CG8421-PB CG8421-PG CG8421-PH CG8421-PI CG8421-PJ CG8421-PK CG8421-PL aspartyl beta-hydroxylase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 52E5-52E5

mRNA and Protein(s)

mRNA	Protein	Name
NM_079033.5	NP_523757.1	aspartyl beta-hydroxylase, isoform B
NM_166140.4	NP_725525.1	aspartyl beta-hydroxylase, isoform A
NM_166141.4	NP_725526.1	aspartyl beta-hydroxylase, isoform K
NM_001259429.2	NP_001246358.1	aspartyl beta-hydroxylase, isoform I
NM_001274091.1	NP_001261020.1	aspartyl beta-hydroxylase, isoform L
NM_001259428.2	NP_001246357.1	aspartyl beta-hydroxylase, isoform H
NM_001259430.2	NP_001246359.1	aspartyl beta-hydroxylase, isoform J
NM_176185.4	NP_788365.2	aspartyl beta-hydroxylase, isoform G

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Asph gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Asph cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa10165	NM_176185.4 Latest version!	Drosophila melanogaster aspartyl beta-hydroxylase, transcript variant G (Asph), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OFa10166	NM_079033.5 Latest version!	Drosophila melanogaster aspartyl beta-hydroxylase, transcript variant B (Asph), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OFa10167	NM_166140.4 Latest version!	Drosophila melanogaster aspartyl beta-hydroxylase, transcript variant A (Asph), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OFa10168	NM_166141.4 Latest version!	Drosophila melanogaster aspartyl beta-hydroxylase, transcript variant K (Asph), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OFa12293	NM_001274091.1 Latest version!	Drosophila melanogaster aspartyl beta-hydroxylase, transcript variant L (Asph), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa12294	NM_001259429.2 Latest version!	Drosophila melanogaster aspartyl beta-hydroxylase, transcript variant I (Asph), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OFa12295	NM_001259430.2 Latest version!	Drosophila melanogaster aspartyl beta-hydroxylase, transcript variant J (Asph), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OFa12296	NM_001259428.2 Latest version!	Drosophila melanogaster aspartyl beta-hydroxylase, transcript variant H (Asph), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Asph ( NM_176185.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_788365.2 Drosophila melanogaster aspartyl beta-hydroxylase, transcript variant G (Asph), mRNA.

Price & Availability↑

CloneID	OFa10165
Clone ID Related Accession (Same CDS sequence)	NM_176185.4
Accession Version	NM_176185.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	aspartyl beta-hydroxylase, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_176185.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGGCG ATGTGCAGCC GCGTAAGCGC AAGGATAAGC GCCGAAAACG CGATGACGAC 
GAATCTTCGC ACGGCGTTCA CATTACAAAG ATGGGAAACG AAGATCTCCA CCTGCATGTG 
CACCACGATC ACGGAACTGG CGGTCACTGG TGCGCCAAGA TTATATTCTT CGCCCTGATG 
GCAGTGCTCC TCGGTTTGGT GGGTCTGATC ATCATGGAGA ATCGCGGACT GGAGGATCTG 
GACACTCCTC TGTCGGAGTC TAGGTTCTCG AAAGTTTTTG ATGGCTGGGT GGACGAACAT 
CGAGATGAGC ATGATGGTCA CGATGTGCAG GAACCCTCGG GAGAGGCGTT AGATGATCAC 
GATGAACACG ACGACCATGA TGATCATGAA GACGAAGACG AAGAGCCCCT CACAGAAGAG 
TTAGAAGAAG AACTGGAGGA AGAGGAAGAG CCAACTGAGG AGGATGAGCC AGCGGCAGAT 
GAAGAATATG AGGAGGATGA AGATGAGGAG AACAATGCGG GTGAAAACAT AACTGCCGAG 
GATGCGGAGG AGGAGGAAGA GGAGGAGGAT AACGATGATG AAGGCACCGT AGAGGCAACT 
GTAGAAGCTA CCACTGAAGC CACTACGGAA GCCACTGGCG AATATGAAGC TGAAGAAGAT 
GATGAGGACG AAGCAGCAGC AGATGATGAT GCAGTAGAGT CCACTGAAGC GCCGCTCTCG 
AAAGCTGAGG AGCAGGAAGA TGATGAGGAT GAGGATGAGG AAGAGCAAGA GATCGAAGAA 
TCAGTAGAGC CGGAAAGATC CCAATATGCG GCACAAAGTG CTCCCGCCAA AGATAATAAT 
GACGACGACG ATGATGATGA TCAAGACAAA AACGATGCAG ATGATGGGGA TGACGACGAA 
TTTGAGTCCC TGGCTCAGCA ATTCGAAAAT GATCCCGAGG ATACAGTGCC AGCAAAAGCT 
GTAGAGAGTG AGGAGCAGGA TCAAGACCAG GCTGATGAGG AGGAGCCCAA GGAAGAGGGC 
TCCTCTTGGT TGGCATCACT TGCCGTTAAA TTTGGCGTTG GCGTTGCACT TGCCTTAGTT 
TCACGCCTTG TATTGATAAG GAAAAGTCCA AATACAACTG AGGATGAGCC CGCACCAGAG 
ACTATATTCA GGCGAAGATT GACGATTGCC ACTGCTGAGG ATCACATACC GGATGATGTG 
GAGGAGCTGC CCCCTTTGGA CGATGAATAT TCCGAGGAAG AAATCGAAAT CGAAGAGGAA 
ATCGAGGTGG AAATCAGTGA TATTGAGGAG GAGGAGGAGG AAGTACGTCA CGATAACGAC 
GACAACGTGG CCTCCATGGC CAGTTATGTG CCCGAGACAT TTGAGCAACT GAGCGCCATG 
TACAAATACG CCCAGGAGCC TACGAAGGAT GCCAAACCCG AACAGAAAGA AAAGGAGCCG 
GAAAAGCCAG TAGGTCACAG CGATATCTAC GTGGAATACG AAGATGGTGC TATCGAGGAT 
TACGAGCATG ACGGAGTTAG CGATGAGGAG ATCACCGACG AGGAGGACGA AATCTCCGAC 
GTGGACGACG CCGATCTGAT GAACCGGCTG GAAGCGAAAT ACGGTCGTCT GCCCGTCAAG 
GAGTTTGAGA GCGATCCGGA CTCTGACGAC CCCAGCTGGA CACGTAATTA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788365.2
CDS	119..1792
Translation	MSGDVQPRKRKDKRRKRDDDESSHGVHITKMGNEDLHLHVHHDHGTGGHWCAKIIFFALMAVLLGLVGLIIMENRGLEDLDTPLSESRFSKVFDGWVDEHRDEHDGHDVQEPSGEALDDHDEHDDHDDHEDEDEEPLTEELEEELEEEEEPTEEDEPAADEEYEEDEDEENNAGENITAEDAEEEEEEEDNDDEGTVEATVEATTEATTEATGEYEAEEDDEDEAAADDDAVESTEAPLSKAEEQEDDEDEDEEEQEIEESVEPERSQYAAQSAPAKDNNDDDDDDDQDKNDADDGDDDEFESLAQQFENDPEDTVPAKAVESEEQDQDQADEEEPKEEGSSWLASLAVKFGVGVALALVSRLVLIRKSPNTTEDEPAPETIFRRRLTIATAEDHIPDDVEELPPLDDEYSEEEIEIEEEIEVEISDIEEEEEEVRHDNDDNVASMASYVPETFEQLSAMYKYAQEPTKDAKPEQKEKEPEKPVGHSDIYVEYEDGAIEDYEHDGVSDEEITDEEDEISDVDDADLMNRLEAKYGRLPVKEFESDPDSDDPSWTRNY

Target ORF information:

RefSeq Version	NM_176185.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster aspartyl beta-hydroxylase, transcript variant G (Asph), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176185.4

ORF Insert Sequence:

ATGTCGGGCG ATGTGCAGCC GCGTAAGCGC AAGGATAAGC GCCGAAAACG CGATGACGAC 
GAATCTTCGC ACGGCGTTCA CATTACAAAG ATGGGAAACG AAGATCTCCA CCTGCATGTG 
CACCACGATC ACGGAACTGG CGGTCACTGG TGCGCCAAGA TTATATTCTT CGCCCTGATG 
GCAGTGCTCC TCGGTTTGGT GGGTCTGATC ATCATGGAGA ATCGCGGACT GGAGGATCTG 
GACACTCCTC TGTCGGAGTC TAGGTTCTCG AAAGTTTTTG ATGGCTGGGT GGACGAACAT 
CGAGATGAGC ATGATGGTCA CGATGTGCAG GAACCCTCGG GAGAGGCGTT AGATGATCAC 
GATGAACACG ACGACCATGA TGATCATGAA GACGAAGACG AAGAGCCCCT CACAGAAGAG 
TTAGAAGAAG AACTGGAGGA AGAGGAAGAG CCAACTGAGG AGGATGAGCC AGCGGCAGAT 
GAAGAATATG AGGAGGATGA AGATGAGGAG AACAATGCGG GTGAAAACAT AACTGCCGAG 
GATGCGGAGG AGGAGGAAGA GGAGGAGGAT AACGATGATG AAGGCACCGT AGAGGCAACT 
GTAGAAGCTA CCACTGAAGC CACTACGGAA GCCACTGGCG AATATGAAGC TGAAGAAGAT 
GATGAGGACG AAGCAGCAGC AGATGATGAT GCAGTAGAGT CCACTGAAGC GCCGCTCTCG 
AAAGCTGAGG AGCAGGAAGA TGATGAGGAT GAGGATGAGG AAGAGCAAGA GATCGAAGAA 
TCAGTAGAGC CGGAAAGATC CCAATATGCG GCACAAAGTG CTCCCGCCAA AGATAATAAT 
GACGACGACG ATGATGATGA TCAAGACAAA AACGATGCAG ATGATGGGGA TGACGACGAA 
TTTGAGTCCC TGGCTCAGCA ATTCGAAAAT GATCCCGAGG ATACAGTGCC AGCAAAAGCT 
GTAGAGAGTG AGGAGCAGGA TCAAGACCAG GCTGATGAGG AGGAGCCCAA GGAAGAGGGC 
TCCTCTTGGT TGGCATCACT TGCCGTTAAA TTTGGCGTTG GCGTTGCACT TGCCTTAGTT 
TCACGCCTTG TATTGATAAG GAAAAGTCCA AATACAACTG AGGATGAGCC CGCACCAGAG 
ACTATATTCA GGCGAAGATT GACGATTGCC ACTGCTGAGG ATCACATACC GGATGATGTG 
GAGGAGCTGC CCCCTTTGGA CGATGAATAT TCCGAGGAAG AAATCGAAAT CGAAGAGGAA 
ATCGAGGTGG AAATCAGTGA TATTGAGGAG GAGGAGGAGG AAGTACGTCA CGATAACGAC 
GACAACGTGG CCTCCATGGC CAGTTATGTG CCCGAGACAT TTGAGCAACT GAGCGCCATG 
TACAAATACG CCCAGGAGCC TACGAAGGAT GCCAAACCCG AACAGAAAGA AAAGGAGCCG 
GAAAAGCCAG TAGGTCACAG CGATATCTAC GTGGAATACG AAGATGGTGC TATCGAGGAT 
TACGAGCATG ACGGAGTTAG CGATGAGGAG ATCACCGACG AGGAGGACGA AATCTCCGAC 
GTGGACGACG CCGATCTGAT GAACCGGCTG GAAGCGAAAT ACGGTCGTCT GCCCGTCAAG 
GAGTTTGAGA GCGATCCGGA CTCTGACGAC CCCAGCTGGA CACGTAATTA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asph ( NM_079033.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523757.1 Drosophila melanogaster aspartyl beta-hydroxylase, transcript variant B (Asph), mRNA.

Price & Availability↑

CloneID	OFa10166
Clone ID Related Accession (Same CDS sequence)	NM_079033.5
Accession Version	NM_079033.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	aspartyl beta-hydroxylase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_079033.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGGCG ATGTGCAGCC GCGTAAGCGC AAGGATAAGC GCCGAAAACG CGATGACGAC 
GAATCTTCGC ACGGCGTTCA CATTACAAAG ATGGGAAACG AAGATCTCCA CCTGCATGTG 
CACCACGATC ACGGAACTGG CGGTCACTGG TGCGCCAAGA TTATATTCTT CGCCCTGATG 
GCAGTGCTCC TCGGTTTGGT GGGTCTGATC ATCATGGAGA ATCGCGGACT GGAGGATCTG 
GACACTCCTC TGTCGGAGTC TAGGTTCTCG AAAGTTTTTG ATGGCTGGGT GGACGAACAT 
CGAGATGAGC ATGATGGTCA CGATGTGCAG GAACCCTCGG GAGAGGCGTT AGATGATCAC 
GATGAACACG ACGACCATGA TGATCATGAA GACGAAGACG AAGAGCCCCT CACAGAAGAG 
TTAGAAGAAG AACTGGAGGA AGAGGAAGAG CCAACTGAGG AGGATGAGCC AGCGGCAGAT 
GAAGAATATG AGGAGGATGA AGATGAGGAG AACAATGCGG GTGAAAACAT AACTGCCGAG 
GATGCGGAGG AGGAGGAAGA GGAGGAGGAT AACGATGATG AAGGCACCGT AGAGGCAACT 
GTAGAAGCTA CCACTGAAGC CACTACGGAA GCCACTGGCG AATATGAAGC TGAAGAAGAT 
GATGAGGACG AAGCAGCAGC AGATGATGAT GCAGTAGAGT CCACTGAAGC GCCGCTCTCG 
AAAGCTGAGG AGCAGGAAGA TGATGAGGAT GAGGATGAGG AAGAGCAAGA GATCGAAGAA 
TCAGTAGAGC CGGAAAGATC CCAATATGCG GCACAAAGTG CTCCCGCCAA AGATAATAAT 
GACGACGACG ATGATGATGA TCAAGACAAA AACGATGCAG ATGATGGGGA TGACGACGAA 
TTTGAGTCCC TGGCTCAGCA ATTCGAAAAT GATCCCGAGG ATACAGTGCC AGCAAAAGCT 
GTAGAGAGTG AGGAGCAGGA TCAAGACCAG GCTGATGAGG AGGAGCCCAA GGAAGAGGGC 
TCCTCTTGGT TGGCATCACA AATAAAGCCG AAAGAAGCTG CACCTGCTCC CGCAGAGAAA 
GAGGATCCTT TCGAGCAAGA GTTGCGCAAG GCAAACGAGG AGATGATTAG GGAGCTCAAC 
AATCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523757.1
CDS	119..1267
Translation	MSGDVQPRKRKDKRRKRDDDESSHGVHITKMGNEDLHLHVHHDHGTGGHWCAKIIFFALMAVLLGLVGLIIMENRGLEDLDTPLSESRFSKVFDGWVDEHRDEHDGHDVQEPSGEALDDHDEHDDHDDHEDEDEEPLTEELEEELEEEEEPTEEDEPAADEEYEEDEDEENNAGENITAEDAEEEEEEEDNDDEGTVEATVEATTEATTEATGEYEAEEDDEDEAAADDDAVESTEAPLSKAEEQEDDEDEDEEEQEIEESVEPERSQYAAQSAPAKDNNDDDDDDDQDKNDADDGDDDEFESLAQQFENDPEDTVPAKAVESEEQDQDQADEEEPKEEGSSWLASQIKPKEAAPAPAEKEDPFEQELRKANEEMIRELNNH

Target ORF information:

RefSeq Version	NM_079033.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster aspartyl beta-hydroxylase, transcript variant B (Asph), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079033.5

ORF Insert Sequence:

ATGTCGGGCG ATGTGCAGCC GCGTAAGCGC AAGGATAAGC GCCGAAAACG CGATGACGAC 
GAATCTTCGC ACGGCGTTCA CATTACAAAG ATGGGAAACG AAGATCTCCA CCTGCATGTG 
CACCACGATC ACGGAACTGG CGGTCACTGG TGCGCCAAGA TTATATTCTT CGCCCTGATG 
GCAGTGCTCC TCGGTTTGGT GGGTCTGATC ATCATGGAGA ATCGCGGACT GGAGGATCTG 
GACACTCCTC TGTCGGAGTC TAGGTTCTCG AAAGTTTTTG ATGGCTGGGT GGACGAACAT 
CGAGATGAGC ATGATGGTCA CGATGTGCAG GAACCCTCGG GAGAGGCGTT AGATGATCAC 
GATGAACACG ACGACCATGA TGATCATGAA GACGAAGACG AAGAGCCCCT CACAGAAGAG 
TTAGAAGAAG AACTGGAGGA AGAGGAAGAG CCAACTGAGG AGGATGAGCC AGCGGCAGAT 
GAAGAATATG AGGAGGATGA AGATGAGGAG AACAATGCGG GTGAAAACAT AACTGCCGAG 
GATGCGGAGG AGGAGGAAGA GGAGGAGGAT AACGATGATG AAGGCACCGT AGAGGCAACT 
GTAGAAGCTA CCACTGAAGC CACTACGGAA GCCACTGGCG AATATGAAGC TGAAGAAGAT 
GATGAGGACG AAGCAGCAGC AGATGATGAT GCAGTAGAGT CCACTGAAGC GCCGCTCTCG 
AAAGCTGAGG AGCAGGAAGA TGATGAGGAT GAGGATGAGG AAGAGCAAGA GATCGAAGAA 
TCAGTAGAGC CGGAAAGATC CCAATATGCG GCACAAAGTG CTCCCGCCAA AGATAATAAT 
GACGACGACG ATGATGATGA TCAAGACAAA AACGATGCAG ATGATGGGGA TGACGACGAA 
TTTGAGTCCC TGGCTCAGCA ATTCGAAAAT GATCCCGAGG ATACAGTGCC AGCAAAAGCT 
GTAGAGAGTG AGGAGCAGGA TCAAGACCAG GCTGATGAGG AGGAGCCCAA GGAAGAGGGC 
TCCTCTTGGT TGGCATCACA AATAAAGCCG AAAGAAGCTG CACCTGCTCC CGCAGAGAAA 
GAGGATCCTT TCGAGCAAGA GTTGCGCAAG GCAAACGAGG AGATGATTAG GGAGCTCAAC 
AATCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asph ( NM_166140.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725525.1 Drosophila melanogaster aspartyl beta-hydroxylase, transcript variant A (Asph), mRNA.

Price & Availability↑

CloneID	OFa10167
Clone ID Related Accession (Same CDS sequence)	NM_166140.4
Accession Version	NM_166140.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	aspartyl beta-hydroxylase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_166140.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGGCG ATGTGCAGCC GCGTAAGCGC AAGGATAAGC GCCGAAAACG CGATGACGAC 
GAATCTTCGC ACGGCGTTCA CATTACAAAG ATGGGAAACG AAGATCTCCA CCTGCATGTG 
CACCACGATC ACGGAACTGG CGGTCACTGG TGCGCCAAGA TTATATTCTT CGCCCTGATG 
GCAGTGCTCC TCGGTTTGGT GGGTCTGATC ATCATGGAGA ATCGCGGACT GGAGGATCTG 
GACACTCCTC TGTCGGAGTC TAGGTTCTCG AAAGTTTTTG ATGGCTGGGT GGACGAACAT 
CGAGATGAGC ATGATGGTCA CGATGTGCAG GAACCCTCGG GAGAGGCGTT AGATGATCAC 
GATGAACACG ACGACCATGA TGATCATGAA GACGAAGACG AAGAGCCCCT CACAGAAGAG 
TTAGAAGAAG AACTGGAGGA AGAGGAAGAG CCAACTGAGG AGGATGAGCC AGCGGCAGAT 
GAAGAATATG AGGAGGATGA AGATGAGGAG AACAATGCGG GTGAAAACAT AACTGCCGAG 
GATGCGGAGG AGGAGGAAGA GGAGGAGGAT AACGATGATG AAGGCACCGT AGAGGCAACT 
GTAGAAGCTA CCACTGAAGC CACTACGGAA GCCACTGGCG AATATGAAGC TGAAGAAGAT 
GATGAGGACG AAGCAGCAGC AGATGATGAT GCAGTAGAGT CCACTGAAGC GCCGCTCTCG 
AAAGCTGAGG AGCAGGAAGA TGATGAGGAT GAGGATGAGG AAGAGCAAGA GATCGAAGAA 
TCAGTAGAGC CGGAAAGATC CCAATATGCG GCACAAAGTG CTCCCGCCAA AGATAATAAT 
GACGACGACG ATGATGATGA TCAAGACAAA AACGATGCAG ATGATGGGGA TGACGACGAA 
TTTGAGTCCC TGGCTCAGCA ATTCGAAAAT GATCCCGAGG ATACAGTGCC AGCAAAAGCT 
GTAGAGAGTG AGGAGCAGGA TCAAGACCAG GCTGATGAGG AGGAGCCCAA GGAAGAGGGC 
TCCTCTTGGT TGGCATCACA AATAAAGCCG AAAGAAGCTG CACCTGCTCC CGCAGAGAAA 
GAGGATCCTT TCGAGCAAGA GTTGCGCAAG GCAAACGAGG AGATGATTAG GGAGAACTAT 
GCCCAAGCCC TGCGTTCCTT TAACACGCTC ACCACCAACT TTGCCCACGA GCCATCGGCC 
CATTTGGGAA GAGCTCGACT TCTGGAACTA CTGGCCAAAA AGGAGCGCAG CAATCAGCGC 
CTGTGGGAAG CCATCGATGC TTACAAAAGA TATTTGGCCT TTGGCGAGCT TGTAGCCAGC 
AATCAGGAAT TTCAAACCGC CGGCGAAAGC TGCATCGAGA ATTTGAGATT TTTGGGTCAC 
CACCGACAGG CAACTACCAT CCATGAGCTG CTCATCAATC GTTTGCCTGA GGATCCACGG 
CTTCGTAACC AACTATCACT CACCTATCTT ATGGTAAACA ATCTTCAGCA GGTTGAAAAG 
GTGGCTGTGG AGACCCTGAA ACTTTGGCCC AACAATGCAG TGGCTCAACT TCATTATGGA 
CTAGCACTCA GACAGTTCCA TGCTGATTAC GCCAAGGCGC TGCCTTATCT AAAATATGCC 
GTGGAGTCTG GAGAGGAGGG CACGCAAGAG GCTTTCTTCT ATTTGTCGCT GGGCGAGACC 
ATGCAGCGCC TGTCCATGAA ATCAGAAGCT CTTGAGGTGT ACGGTAAAGG TGTTGCCAAA 
GGATTCTTTG CCAGCCTCTA TCAGAGATCG CTGTACAATG AGCCCAGGTT GAGAGCACAG 
CCATTTTGGC AGCCCAAGGA AACGGGCTAT GAAAGGCAGC TGGAGAAGCT GACGCTCAAT 
TGGCGTGCCA TTCGCGATGA AGGACTAGCA CTGCTGGGAA GAAGTGGATT CTTCGAGGAT 
GAAGCGGAAC TGCTGCGCGA CAAAGGAGTG TGGCAACAGT ATGAGCTATA TGCCCAGGGT 
CGTCGGGTGA AGGACAACTG CCGCAGGGCC CCAATCACCT GTAGCCTGCT GGAGGAATTT 
CCCGAGTCCG CCGGCTGTCG ACGTGGCCAA GTGAAGTTTA GTGTTATGCA GGCCAAGACG 
CATGTGTGGC CGCATTGTGG ACCCACCAAT TGCCGACTGA GGGCCCACCT CACGCTAGCT 
GCTCCGGAGC CGGAAAAAGC ATCACTCCGC GTGGCGGAGC AGGAAAGAAC CTGGCGTGAG 
GGAGAACTGT TCATATTTGA TGATAGCTTC GAACACGAGG TGTGGCACAA CGGAAGCCAG 
TCACGCCTTG TGCTCATCCT AGATATGTGG CATCCGCAAC TGAGTGCCGC CCAGCGCCGC 
AGTTTATCAC CCATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725525.1
CDS	119..2476
Translation	MSGDVQPRKRKDKRRKRDDDESSHGVHITKMGNEDLHLHVHHDHGTGGHWCAKIIFFALMAVLLGLVGLIIMENRGLEDLDTPLSESRFSKVFDGWVDEHRDEHDGHDVQEPSGEALDDHDEHDDHDDHEDEDEEPLTEELEEELEEEEEPTEEDEPAADEEYEEDEDEENNAGENITAEDAEEEEEEEDNDDEGTVEATVEATTEATTEATGEYEAEEDDEDEAAADDDAVESTEAPLSKAEEQEDDEDEDEEEQEIEESVEPERSQYAAQSAPAKDNNDDDDDDDQDKNDADDGDDDEFESLAQQFENDPEDTVPAKAVESEEQDQDQADEEEPKEEGSSWLASQIKPKEAAPAPAEKEDPFEQELRKANEEMIRENYAQALRSFNTLTTNFAHEPSAHLGRARLLELLAKKERSNQRLWEAIDAYKRYLAFGELVASNQEFQTAGESCIENLRFLGHHRQATTIHELLINRLPEDPRLRNQLSLTYLMVNNLQQVEKVAVETLKLWPNNAVAQLHYGLALRQFHADYAKALPYLKYAVESGEEGTQEAFFYLSLGETMQRLSMKSEALEVYGKGVAKGFFASLYQRSLYNEPRLRAQPFWQPKETGYERQLEKLTLNWRAIRDEGLALLGRSGFFEDEAELLRDKGVWQQYELYAQGRRVKDNCRRAPITCSLLEEFPESAGCRRGQVKFSVMQAKTHVWPHCGPTNCRLRAHLTLAAPEPEKASLRVAEQERTWREGELFIFDDSFEHEVWHNGSQSRLVLILDMWHPQLSAAQRRSLSPI

Target ORF information:

RefSeq Version	NM_166140.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster aspartyl beta-hydroxylase, transcript variant A (Asph), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166140.4

ORF Insert Sequence:

ATGTCGGGCG ATGTGCAGCC GCGTAAGCGC AAGGATAAGC GCCGAAAACG CGATGACGAC 
GAATCTTCGC ACGGCGTTCA CATTACAAAG ATGGGAAACG AAGATCTCCA CCTGCATGTG 
CACCACGATC ACGGAACTGG CGGTCACTGG TGCGCCAAGA TTATATTCTT CGCCCTGATG 
GCAGTGCTCC TCGGTTTGGT GGGTCTGATC ATCATGGAGA ATCGCGGACT GGAGGATCTG 
GACACTCCTC TGTCGGAGTC TAGGTTCTCG AAAGTTTTTG ATGGCTGGGT GGACGAACAT 
CGAGATGAGC ATGATGGTCA CGATGTGCAG GAACCCTCGG GAGAGGCGTT AGATGATCAC 
GATGAACACG ACGACCATGA TGATCATGAA GACGAAGACG AAGAGCCCCT CACAGAAGAG 
TTAGAAGAAG AACTGGAGGA AGAGGAAGAG CCAACTGAGG AGGATGAGCC AGCGGCAGAT 
GAAGAATATG AGGAGGATGA AGATGAGGAG AACAATGCGG GTGAAAACAT AACTGCCGAG 
GATGCGGAGG AGGAGGAAGA GGAGGAGGAT AACGATGATG AAGGCACCGT AGAGGCAACT 
GTAGAAGCTA CCACTGAAGC CACTACGGAA GCCACTGGCG AATATGAAGC TGAAGAAGAT 
GATGAGGACG AAGCAGCAGC AGATGATGAT GCAGTAGAGT CCACTGAAGC GCCGCTCTCG 
AAAGCTGAGG AGCAGGAAGA TGATGAGGAT GAGGATGAGG AAGAGCAAGA GATCGAAGAA 
TCAGTAGAGC CGGAAAGATC CCAATATGCG GCACAAAGTG CTCCCGCCAA AGATAATAAT 
GACGACGACG ATGATGATGA TCAAGACAAA AACGATGCAG ATGATGGGGA TGACGACGAA 
TTTGAGTCCC TGGCTCAGCA ATTCGAAAAT GATCCCGAGG ATACAGTGCC AGCAAAAGCT 
GTAGAGAGTG AGGAGCAGGA TCAAGACCAG GCTGATGAGG AGGAGCCCAA GGAAGAGGGC 
TCCTCTTGGT TGGCATCACA AATAAAGCCG AAAGAAGCTG CACCTGCTCC CGCAGAGAAA 
GAGGATCCTT TCGAGCAAGA GTTGCGCAAG GCAAACGAGG AGATGATTAG GGAGAACTAT 
GCCCAAGCCC TGCGTTCCTT TAACACGCTC ACCACCAACT TTGCCCACGA GCCATCGGCC 
CATTTGGGAA GAGCTCGACT TCTGGAACTA CTGGCCAAAA AGGAGCGCAG CAATCAGCGC 
CTGTGGGAAG CCATCGATGC TTACAAAAGA TATTTGGCCT TTGGCGAGCT TGTAGCCAGC 
AATCAGGAAT TTCAAACCGC CGGCGAAAGC TGCATCGAGA ATTTGAGATT TTTGGGTCAC 
CACCGACAGG CAACTACCAT CCATGAGCTG CTCATCAATC GTTTGCCTGA GGATCCACGG 
CTTCGTAACC AACTATCACT CACCTATCTT ATGGTAAACA ATCTTCAGCA GGTTGAAAAG 
GTGGCTGTGG AGACCCTGAA ACTTTGGCCC AACAATGCAG TGGCTCAACT TCATTATGGA 
CTAGCACTCA GACAGTTCCA TGCTGATTAC GCCAAGGCGC TGCCTTATCT AAAATATGCC 
GTGGAGTCTG GAGAGGAGGG CACGCAAGAG GCTTTCTTCT ATTTGTCGCT GGGCGAGACC 
ATGCAGCGCC TGTCCATGAA ATCAGAAGCT CTTGAGGTGT ACGGTAAAGG TGTTGCCAAA 
GGATTCTTTG CCAGCCTCTA TCAGAGATCG CTGTACAATG AGCCCAGGTT GAGAGCACAG 
CCATTTTGGC AGCCCAAGGA AACGGGCTAT GAAAGGCAGC TGGAGAAGCT GACGCTCAAT 
TGGCGTGCCA TTCGCGATGA AGGACTAGCA CTGCTGGGAA GAAGTGGATT CTTCGAGGAT 
GAAGCGGAAC TGCTGCGCGA CAAAGGAGTG TGGCAACAGT ATGAGCTATA TGCCCAGGGT 
CGTCGGGTGA AGGACAACTG CCGCAGGGCC CCAATCACCT GTAGCCTGCT GGAGGAATTT 
CCCGAGTCCG CCGGCTGTCG ACGTGGCCAA GTGAAGTTTA GTGTTATGCA GGCCAAGACG 
CATGTGTGGC CGCATTGTGG ACCCACCAAT TGCCGACTGA GGGCCCACCT CACGCTAGCT 
GCTCCGGAGC CGGAAAAAGC ATCACTCCGC GTGGCGGAGC AGGAAAGAAC CTGGCGTGAG 
GGAGAACTGT TCATATTTGA TGATAGCTTC GAACACGAGG TGTGGCACAA CGGAAGCCAG 
TCACGCCTTG TGCTCATCCT AGATATGTGG CATCCGCAAC TGAGTGCCGC CCAGCGCCGC 
AGTTTATCAC CCATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asph ( NM_166141.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725526.1 Drosophila melanogaster aspartyl beta-hydroxylase, transcript variant K (Asph), mRNA.

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CloneID	OFa10168
Clone ID Related Accession (Same CDS sequence)	NM_166141.4
Accession Version	NM_166141.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	aspartyl beta-hydroxylase, isoform K
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_166141.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGGCG ATGTGCAGCC GCGTAAGCGC AAGGATAAGC GCCGAAAACG CGATGACGAC 
GAATCTTCGC ACGGCGTTCA CATTACAAAG ATGGGAAACG AAGATCTCCA CCTGCATGTG 
CACCACGATC ACGGAACTGG CGGTCACTGG TGCGCCAAGA TTATATTCTT CGCCCTGATG 
GCAGTGCTCC TCGGTTTGGT GGGTCTGATC ATCATGGAGA ATCGCGGACT GGAGGATCTG 
GACACTCCTC TGTCGGAGTC TAGGTTCTCG AAAGTTTTTG ATGGCTGGGT GGACGAACAT 
CGAGATGAGC ATGATGGTCA CGATGTGCAG GAACCCTCGG GAGAGGCGTT AGATGATCAC 
GATGAACACG ACGACCATGA TGATCATGAA GACGAAGACG AAGAGCCCCT CACAGAAGAG 
TTAGAAGAAG AACTGGAGGA AGAGGAAGAG CCAACTGAGG AGGATGAGCC AGCGGCAGAT 
GAAGAATATG AGGAGGATGA AGATGAGGAG AACAATGCGG GTGAAAACAT AACTGCCGAG 
GATGCGGAGG AGGAGGAAGA GGAGGAGGAT AACGATGATG AAGGCACCGT AGAGGCAACT 
GTAGAAGCTA CCACTGAAGC CACTACGGAA GCCACTGGCG AATATGAAGC TGAAGAAGAT 
GATGAGGACG AAGCAGCAGC AGATGATGAT GCAGTAGAGT CCACTGAAGC GCCGCTCTCG 
AAAGCTGAGG AGGAAGATGA TGAGGATGAG GATGAGGAAG AGCAAGAGAT CGAAGAATCA 
GTAGAGCCGG AAAGATCCCA ATATGCGGCA CAAAGTGCTC CCGCCAAAGA TAATAATGAC 
GACGACGATG ATGATGATCA AGACAAAAAC GATGCAGATG ATGGGGATGA CGACGAATTT 
GAGTCCCTGG CTCAGCAATT CGAAAATGAT CCCGAGGATA CAGTGCCAGC AAAAGCTGTA 
GAGAGTGAGG AGCAGGATCA AGACCAGGCT GATGAGGAGG AGCCCAAGGA AGAGGGCTCC 
TCTTGGTTGG CATCACTTGC CGTTAAATTT GGCGTTGGCG TTGCACTTGC CTTAGTTTCA 
CGCCTTGTAT TGATAAGGAA AAGTCCAAAT ACAACTGAGG ATGAGCCCGC ACCAGAGACT 
ATATTCAGGC GAAGATTGAC GATTGCCACT GCTGAGGATC ACATACCGGA TGATGTGGAG 
GAGCTGCCCC CTTTGGACGA TGAATATTCC GAGGAAGAAA TCGAAATCGA AGAGGAAATC 
GAGGTGGAAA TCAGTGATAT TGAGGAGGAG GAGGAGGAAG TACGTCACGA TAACGACGAC 
AACGTGGCCT CCATGGCCAG TTATGTGCCC GAGACATTTG AGCAACTGAG CGCCATGTAC 
AAATACGCCC AGGAGCCTAC GAAGGATGCC AAACCCGAAC AGAAAGAAAA GGAGCCGGAA 
AAGCCAGTAG GTCACAGCGA TATCTACGTG GAATACGAAG ATGGTGCTAT CGAGGATTAC 
GAGCATGACG GAGTTAGCGA TGAGGAGATC ACCGACGAGG AGGACGAAAT CTCCGACGTG 
GACGACGCCG ATCTGATGAA CCGGCTGGAA GCGAAATACG GTCGTCTGCC CGTCAAGGAG 
TTTGAGAGCG ATCCGGACTC TGACGACCCC AGCTGGACAC GTAATTACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725526.1
CDS	119..1789
Translation	MSGDVQPRKRKDKRRKRDDDESSHGVHITKMGNEDLHLHVHHDHGTGGHWCAKIIFFALMAVLLGLVGLIIMENRGLEDLDTPLSESRFSKVFDGWVDEHRDEHDGHDVQEPSGEALDDHDEHDDHDDHEDEDEEPLTEELEEELEEEEEPTEEDEPAADEEYEEDEDEENNAGENITAEDAEEEEEEEDNDDEGTVEATVEATTEATTEATGEYEAEEDDEDEAAADDDAVESTEAPLSKAEEEDDEDEDEEEQEIEESVEPERSQYAAQSAPAKDNNDDDDDDDQDKNDADDGDDDEFESLAQQFENDPEDTVPAKAVESEEQDQDQADEEEPKEEGSSWLASLAVKFGVGVALALVSRLVLIRKSPNTTEDEPAPETIFRRRLTIATAEDHIPDDVEELPPLDDEYSEEEIEIEEEIEVEISDIEEEEEEVRHDNDDNVASMASYVPETFEQLSAMYKYAQEPTKDAKPEQKEKEPEKPVGHSDIYVEYEDGAIEDYEHDGVSDEEITDEEDEISDVDDADLMNRLEAKYGRLPVKEFESDPDSDDPSWTRNY

Target ORF information:

RefSeq Version	NM_166141.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster aspartyl beta-hydroxylase, transcript variant K (Asph), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166141.4

ORF Insert Sequence:

ATGTCGGGCG ATGTGCAGCC GCGTAAGCGC AAGGATAAGC GCCGAAAACG CGATGACGAC 
GAATCTTCGC ACGGCGTTCA CATTACAAAG ATGGGAAACG AAGATCTCCA CCTGCATGTG 
CACCACGATC ACGGAACTGG CGGTCACTGG TGCGCCAAGA TTATATTCTT CGCCCTGATG 
GCAGTGCTCC TCGGTTTGGT GGGTCTGATC ATCATGGAGA ATCGCGGACT GGAGGATCTG 
GACACTCCTC TGTCGGAGTC TAGGTTCTCG AAAGTTTTTG ATGGCTGGGT GGACGAACAT 
CGAGATGAGC ATGATGGTCA CGATGTGCAG GAACCCTCGG GAGAGGCGTT AGATGATCAC 
GATGAACACG ACGACCATGA TGATCATGAA GACGAAGACG AAGAGCCCCT CACAGAAGAG 
TTAGAAGAAG AACTGGAGGA AGAGGAAGAG CCAACTGAGG AGGATGAGCC AGCGGCAGAT 
GAAGAATATG AGGAGGATGA AGATGAGGAG AACAATGCGG GTGAAAACAT AACTGCCGAG 
GATGCGGAGG AGGAGGAAGA GGAGGAGGAT AACGATGATG AAGGCACCGT AGAGGCAACT 
GTAGAAGCTA CCACTGAAGC CACTACGGAA GCCACTGGCG AATATGAAGC TGAAGAAGAT 
GATGAGGACG AAGCAGCAGC AGATGATGAT GCAGTAGAGT CCACTGAAGC GCCGCTCTCG 
AAAGCTGAGG AGGAAGATGA TGAGGATGAG GATGAGGAAG AGCAAGAGAT CGAAGAATCA 
GTAGAGCCGG AAAGATCCCA ATATGCGGCA CAAAGTGCTC CCGCCAAAGA TAATAATGAC 
GACGACGATG ATGATGATCA AGACAAAAAC GATGCAGATG ATGGGGATGA CGACGAATTT 
GAGTCCCTGG CTCAGCAATT CGAAAATGAT CCCGAGGATA CAGTGCCAGC AAAAGCTGTA 
GAGAGTGAGG AGCAGGATCA AGACCAGGCT GATGAGGAGG AGCCCAAGGA AGAGGGCTCC 
TCTTGGTTGG CATCACTTGC CGTTAAATTT GGCGTTGGCG TTGCACTTGC CTTAGTTTCA 
CGCCTTGTAT TGATAAGGAA AAGTCCAAAT ACAACTGAGG ATGAGCCCGC ACCAGAGACT 
ATATTCAGGC GAAGATTGAC GATTGCCACT GCTGAGGATC ACATACCGGA TGATGTGGAG 
GAGCTGCCCC CTTTGGACGA TGAATATTCC GAGGAAGAAA TCGAAATCGA AGAGGAAATC 
GAGGTGGAAA TCAGTGATAT TGAGGAGGAG GAGGAGGAAG TACGTCACGA TAACGACGAC 
AACGTGGCCT CCATGGCCAG TTATGTGCCC GAGACATTTG AGCAACTGAG CGCCATGTAC 
AAATACGCCC AGGAGCCTAC GAAGGATGCC AAACCCGAAC AGAAAGAAAA GGAGCCGGAA 
AAGCCAGTAG GTCACAGCGA TATCTACGTG GAATACGAAG ATGGTGCTAT CGAGGATTAC 
GAGCATGACG GAGTTAGCGA TGAGGAGATC ACCGACGAGG AGGACGAAAT CTCCGACGTG 
GACGACGCCG ATCTGATGAA CCGGCTGGAA GCGAAATACG GTCGTCTGCC CGTCAAGGAG 
TTTGAGAGCG ATCCGGACTC TGACGACCCC AGCTGGACAC GTAATTACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asph ( NM_001274091.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261020.1 Drosophila melanogaster aspartyl beta-hydroxylase, transcript variant L (Asph), mRNA.

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CloneID	OFa12293
Clone ID Related Accession (Same CDS sequence)	NM_001274091.1
Accession Version	NM_001274091.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2976bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	aspartyl beta-hydroxylase, isoform L
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGGCG ATGTGCAGCC GCGTAAGCGC AAGGATAAGC GCCGAAAACG CGATGACGAC 
GAATCTTCGC ACGGCGTTCA CATTACAAAG ATGGGAAACG AAGATCTCCA CCTGCATGTG 
CACCACGATC ACGGAACTGG CGGTCACTGG TGCGCCAAGA TTATATTCTT CGCCCTGATG 
GCAGTGCTCC TCGGTTTGGT GGGTCTGATC ATCATGGAGA ATCGCGGACT GGAGGATCTG 
GACACTCCTC TGTCGGAGTC TAGGTTCTCG AAAGTTTTTG ATGGCTGGGT GGACGAACAT 
CGAGATGAGC ATGATGGTCA CGATGTGCAG GAACCCTCGG GAGAGGCGTT AGATGATCAC 
GATGAACACG ACGACCATGA TGATCATGAA GACGAAGAGC CCCTCACAGA AGAGTTAGAA 
GAAGAACTGG AGGAAGAGGA AGAGCCAACT GAGGAGGATG AGCCAGCGGC AGATGAAGAA 
TATGAGGAGG ATGAAGATGA GGAGAACAAT GCGGGTGAAA ACATAACTGC CGAGGATGCG 
GAGGAGGAGG AAGAGGAGGA GGATAACGAT GATGAAGGCA CCGTAGAGGC AACTGTAGAA 
GCTACCACTG AAGCCACTAC GGAAGCCACT GGCGAATATG AAGCTGAAGA AGATGATGAG 
GACGAAGCAG CAGCAGATGA TGATGCAGTA GAGTCCACTG AAGCGCCGCT CTCGAAAGCT 
GAGGAGCAGG AAGATGATGA GGATGAGGAT GAGGAAGAGC AAGAGATCGA AGAATCAGTA 
GAGCCGGAAA GATCCCAATA TGCGGCACAA AGTGCTCCCG CCAAAGATAA TAATGACGAC 
GACGATGATG ATGATCAAGA CAAAAACGAT GCAGATGATG GGGATGACGA CGAATTTGAG 
TCCCTGGCTC AGCAATTCGA AAATGATCCC GAGGATACAG TGCCAGCAAA AGCTGTAGAG 
AGTGAGGAGC AGGATCAAGA CCAGGCTGAT GAGGAGGAGC CCAAGGAAGA GGGCTCCTCT 
TGGTTGGCAT CACTTGCCGT TAAATTTGGC GTTGGCGTTG CACTTGCCTT AGTTTCACGC 
CTTGTATTGA TAAGGAAAAG TCCAAATACA ACTGAGGATG AGCCCGCACC AGAGACTATA 
TTCAGGCGAA GATTGACGAT TGCCACTGCT GAGGATCACA TACCGGATGA TGTGGAGGAG 
CTGCCCCCTT TGGACGATGA ATATTCCGAG GAAGAAATCG AAATCGAAGA GGAAATCGAG 
GTGGAAATCA GTGATATTGA GGAGGAGGAG GAGGAAGTAC GTCACGATAA CGACGACAAC 
GTGGCCTCCA TGGCCAGTTA TGTGCCCGAG ACATTTGAGC AACTGAGCGC CATGTACAAA 
TACGCCCAGG AGCCTACGAA GGATGCCAAA CCCGAACAGA AAGAAAAGGA GCCGGAAAAG 
CCAGTAGGTC ACAGCGATAT CTACGTGGAA TACGAAGATG GTGCTATCGA GGATTACGAG 
CATGACGGAG TTAGCGATGA GGAGATCACC GACGAGGAGG ACGAAATCTC CGACGTGGAC 
GACGCCGATC TGATGAACCG GCTGGAAGCG AAATACGGTC GTCTGCCCGT CAAGGAGTTT 
GAGAGCGATC CGGACTCTGA CGACCCCAGC TGGACACAAA TAAAGCCGAA AGAAGCTGCA 
CCTGCTCCCG CAGAGAAAGA GGATCCTTTC GAGCAAGAGT TGCGCAAGGC AAACGAGGAG 
ATGATTAGGG AGAACTATGC CCAAGCCCTG CGTTCCTTTA ACACGCTCAC CACCAACTTT 
GCCCACGAGC CATCGGCCCA TTTGGGAAGA GCTCGACTTC TGGAACTACT GGCCAAAAAG 
GAGCGCAGCA ATCAGCGCCT GTGGGAAGCC ATCGATGCTT ACAAAAGATA TTTGGCCTTT 
GGCGAGCTTG TAGCCAGCAA TCAGGAATTT CAAACCGCCG GCGAAAGCTG CATCGAGAAT 
TTGAGATTTT TGGGTCACCA CCGACAGGCA ACTACCATCC ATGAGCTGCT CATCAATCGT 
TTGCCTGAGG ATCCACGGCT TCGTAACCAA CTATCACTCA CCTATCTTAT GGTAAACAAT 
CTTCAGCAGG TTGAAAAGGT GGCTGTGGAG ACCCTGAAAC TTTGGCCCAA CAATGCAGTG 
GCTCAACTTC ATTATGGACT AGCACTCAGA CAGTTCCATG CTGATTACGC CAAGGCGCTG 
CCTTATCTAA AATATGCCGT GGAGTCTGGA GAGGAGGGCA CGCAAGAGGC TTTCTTCTAT 
TTGTCGCTGG GCGAGACCAT GCAGCGCCTG TCCATGAAAT CAGAAGCTCT TGAGGTGTAC 
GGTAAAGGTG TTGCCAAAGG ATTCTTTGCC AGCCTCTATC AGAGATCGCT GTACAATGAG 
CCCAGGTTGA GAGCACAGCC ATTTTGGCAG CCCAAGGAAA CGGGCTATGA AAGGCAGCTG 
GAGAAGCTGA CGCTCAATTG GCGTGCCATT CGCGATGAAG GACTAGCACT GCTGGGAAGA 
AGTGGATTCT TCGAGGATGA AGCGGAACTG CTGCGCGACA AAGGAGTGTG GCAACAGTAT 
GAGCTATATG CCCAGGGTCG TCGGGTGAAG GACAACTGCC GCAGGGCCCC AATCACCTGT 
AGCCTGCTGG AGGAATTTCC CGAGTCCGCC GGCTGTCGAC GTGGCCAAGT GAAGTTTAGT 
GTTATGCAGG CCAAGACGCA TGTGTGGCCG CATTGTGGAC CCACCAATTG CCGACTGAGG 
GCCCACCTCA CGCTAGCTGC TCCGGAGCCG GAAAAAGCAT CACTCCGCGT GGCGGAGCAG 
GAAAGAACCT GGCGTGAGGG AGAACTGTTC ATATTTGATG ATAGCTTCGA ACACGAGGTG 
TGGCACAACG GAAGCCAGTC ACGCCTTGTG CTCATCCTAG ATATGTGGCA TCCGCAACTG 
AGTGCCGCCC AGCGCCGCAG TTTATCACCC ATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261020.1
CDS	290..3265
Translation	MSGDVQPRKRKDKRRKRDDDESSHGVHITKMGNEDLHLHVHHDHGTGGHWCAKIIFFALMAVLLGLVGLIIMENRGLEDLDTPLSESRFSKVFDGWVDEHRDEHDGHDVQEPSGEALDDHDEHDDHDDHEDEEPLTEELEEELEEEEEPTEEDEPAADEEYEEDEDEENNAGENITAEDAEEEEEEEDNDDEGTVEATVEATTEATTEATGEYEAEEDDEDEAAADDDAVESTEAPLSKAEEQEDDEDEDEEEQEIEESVEPERSQYAAQSAPAKDNNDDDDDDDQDKNDADDGDDDEFESLAQQFENDPEDTVPAKAVESEEQDQDQADEEEPKEEGSSWLASLAVKFGVGVALALVSRLVLIRKSPNTTEDEPAPETIFRRRLTIATAEDHIPDDVEELPPLDDEYSEEEIEIEEEIEVEISDIEEEEEEVRHDNDDNVASMASYVPETFEQLSAMYKYAQEPTKDAKPEQKEKEPEKPVGHSDIYVEYEDGAIEDYEHDGVSDEEITDEEDEISDVDDADLMNRLEAKYGRLPVKEFESDPDSDDPSWTQIKPKEAAPAPAEKEDPFEQELRKANEEMIRENYAQALRSFNTLTTNFAHEPSAHLGRARLLELLAKKERSNQRLWEAIDAYKRYLAFGELVASNQEFQTAGESCIENLRFLGHHRQATTIHELLINRLPEDPRLRNQLSLTYLMVNNLQQVEKVAVETLKLWPNNAVAQLHYGLALRQFHADYAKALPYLKYAVESGEEGTQEAFFYLSLGETMQRLSMKSEALEVYGKGVAKGFFASLYQRSLYNEPRLRAQPFWQPKETGYERQLEKLTLNWRAIRDEGLALLGRSGFFEDEAELLRDKGVWQQYELYAQGRRVKDNCRRAPITCSLLEEFPESAGCRRGQVKFSVMQAKTHVWPHCGPTNCRLRAHLTLAAPEPEKASLRVAEQERTWREGELFIFDDSFEHEVWHNGSQSRLVLILDMWHPQLSAAQRRSLSPI

Target ORF information:

RefSeq Version	NM_001274091.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster aspartyl beta-hydroxylase, transcript variant L (Asph), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274091.1

ORF Insert Sequence:

ATGTCGGGCG ATGTGCAGCC GCGTAAGCGC AAGGATAAGC GCCGAAAACG CGATGACGAC 
GAATCTTCGC ACGGCGTTCA CATTACAAAG ATGGGAAACG AAGATCTCCA CCTGCATGTG 
CACCACGATC ACGGAACTGG CGGTCACTGG TGCGCCAAGA TTATATTCTT CGCCCTGATG 
GCAGTGCTCC TCGGTTTGGT GGGTCTGATC ATCATGGAGA ATCGCGGACT GGAGGATCTG 
GACACTCCTC TGTCGGAGTC TAGGTTCTCG AAAGTTTTTG ATGGCTGGGT GGACGAACAT 
CGAGATGAGC ATGATGGTCA CGATGTGCAG GAACCCTCGG GAGAGGCGTT AGATGATCAC 
GATGAACACG ACGACCATGA TGATCATGAA GACGAAGAGC CCCTCACAGA AGAGTTAGAA 
GAAGAACTGG AGGAAGAGGA AGAGCCAACT GAGGAGGATG AGCCAGCGGC AGATGAAGAA 
TATGAGGAGG ATGAAGATGA GGAGAACAAT GCGGGTGAAA ACATAACTGC CGAGGATGCG 
GAGGAGGAGG AAGAGGAGGA GGATAACGAT GATGAAGGCA CCGTAGAGGC AACTGTAGAA 
GCTACCACTG AAGCCACTAC GGAAGCCACT GGCGAATATG AAGCTGAAGA AGATGATGAG 
GACGAAGCAG CAGCAGATGA TGATGCAGTA GAGTCCACTG AAGCGCCGCT CTCGAAAGCT 
GAGGAGCAGG AAGATGATGA GGATGAGGAT GAGGAAGAGC AAGAGATCGA AGAATCAGTA 
GAGCCGGAAA GATCCCAATA TGCGGCACAA AGTGCTCCCG CCAAAGATAA TAATGACGAC 
GACGATGATG ATGATCAAGA CAAAAACGAT GCAGATGATG GGGATGACGA CGAATTTGAG 
TCCCTGGCTC AGCAATTCGA AAATGATCCC GAGGATACAG TGCCAGCAAA AGCTGTAGAG 
AGTGAGGAGC AGGATCAAGA CCAGGCTGAT GAGGAGGAGC CCAAGGAAGA GGGCTCCTCT 
TGGTTGGCAT CACTTGCCGT TAAATTTGGC GTTGGCGTTG CACTTGCCTT AGTTTCACGC 
CTTGTATTGA TAAGGAAAAG TCCAAATACA ACTGAGGATG AGCCCGCACC AGAGACTATA 
TTCAGGCGAA GATTGACGAT TGCCACTGCT GAGGATCACA TACCGGATGA TGTGGAGGAG 
CTGCCCCCTT TGGACGATGA ATATTCCGAG GAAGAAATCG AAATCGAAGA GGAAATCGAG 
GTGGAAATCA GTGATATTGA GGAGGAGGAG GAGGAAGTAC GTCACGATAA CGACGACAAC 
GTGGCCTCCA TGGCCAGTTA TGTGCCCGAG ACATTTGAGC AACTGAGCGC CATGTACAAA 
TACGCCCAGG AGCCTACGAA GGATGCCAAA CCCGAACAGA AAGAAAAGGA GCCGGAAAAG 
CCAGTAGGTC ACAGCGATAT CTACGTGGAA TACGAAGATG GTGCTATCGA GGATTACGAG 
CATGACGGAG TTAGCGATGA GGAGATCACC GACGAGGAGG ACGAAATCTC CGACGTGGAC 
GACGCCGATC TGATGAACCG GCTGGAAGCG AAATACGGTC GTCTGCCCGT CAAGGAGTTT 
GAGAGCGATC CGGACTCTGA CGACCCCAGC TGGACACAAA TAAAGCCGAA AGAAGCTGCA 
CCTGCTCCCG CAGAGAAAGA GGATCCTTTC GAGCAAGAGT TGCGCAAGGC AAACGAGGAG 
ATGATTAGGG AGAACTATGC CCAAGCCCTG CGTTCCTTTA ACACGCTCAC CACCAACTTT 
GCCCACGAGC CATCGGCCCA TTTGGGAAGA GCTCGACTTC TGGAACTACT GGCCAAAAAG 
GAGCGCAGCA ATCAGCGCCT GTGGGAAGCC ATCGATGCTT ACAAAAGATA TTTGGCCTTT 
GGCGAGCTTG TAGCCAGCAA TCAGGAATTT CAAACCGCCG GCGAAAGCTG CATCGAGAAT 
TTGAGATTTT TGGGTCACCA CCGACAGGCA ACTACCATCC ATGAGCTGCT CATCAATCGT 
TTGCCTGAGG ATCCACGGCT TCGTAACCAA CTATCACTCA CCTATCTTAT GGTAAACAAT 
CTTCAGCAGG TTGAAAAGGT GGCTGTGGAG ACCCTGAAAC TTTGGCCCAA CAATGCAGTG 
GCTCAACTTC ATTATGGACT AGCACTCAGA CAGTTCCATG CTGATTACGC CAAGGCGCTG 
CCTTATCTAA AATATGCCGT GGAGTCTGGA GAGGAGGGCA CGCAAGAGGC TTTCTTCTAT 
TTGTCGCTGG GCGAGACCAT GCAGCGCCTG TCCATGAAAT CAGAAGCTCT TGAGGTGTAC 
GGTAAAGGTG TTGCCAAAGG ATTCTTTGCC AGCCTCTATC AGAGATCGCT GTACAATGAG 
CCCAGGTTGA GAGCACAGCC ATTTTGGCAG CCCAAGGAAA CGGGCTATGA AAGGCAGCTG 
GAGAAGCTGA CGCTCAATTG GCGTGCCATT CGCGATGAAG GACTAGCACT GCTGGGAAGA 
AGTGGATTCT TCGAGGATGA AGCGGAACTG CTGCGCGACA AAGGAGTGTG GCAACAGTAT 
GAGCTATATG CCCAGGGTCG TCGGGTGAAG GACAACTGCC GCAGGGCCCC AATCACCTGT 
AGCCTGCTGG AGGAATTTCC CGAGTCCGCC GGCTGTCGAC GTGGCCAAGT GAAGTTTAGT 
GTTATGCAGG CCAAGACGCA TGTGTGGCCG CATTGTGGAC CCACCAATTG CCGACTGAGG 
GCCCACCTCA CGCTAGCTGC TCCGGAGCCG GAAAAAGCAT CACTCCGCGT GGCGGAGCAG 
GAAAGAACCT GGCGTGAGGG AGAACTGTTC ATATTTGATG ATAGCTTCGA ACACGAGGTG 
TGGCACAACG GAAGCCAGTC ACGCCTTGTG CTCATCCTAG ATATGTGGCA TCCGCAACTG 
AGTGCCGCCC AGCGCCGCAG TTTATCACCC ATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asph ( NM_001259429.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246358.1 Drosophila melanogaster aspartyl beta-hydroxylase, transcript variant I (Asph), mRNA.

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CloneID	OFa12294
Clone ID Related Accession (Same CDS sequence)	NM_001259429.2
Accession Version	NM_001259429.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	aspartyl beta-hydroxylase, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001259429.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGGCG ATGTGCAGCC GCGTAAGCGC AAGGATAAGC GCCGAAAACG CGATGATCGC 
AAGTCCGAGG GCGATGTGAC TGTTCTGCGT CAGTCCAGTT TTCAAGATGA CGACGAATCT 
TCGCACGGCG TTCACATTAC AAAGATGGGA AACGAAGATC TCCACCTGCA TGTGCACCAC 
GATCACGGAA CTGGCGGTCA CTGGTGCGCC AAGATTATAT TCTTCGCCCT GATGGCAGTG 
CTCCTCGGTT TGGTGGGTCT GATCATCATG GAGAATCGCG GACTGGAGGA TCTGGACACT 
CCTCTGTCGG AGTCTAGGTT CTCGAAAGTT TTTGATGGCT GGGTGGACGA ACATCGAGAT 
GAGCATGATG GTCACGATGT GCAGGAACCC TCGGGAGAGG CGTTAGATGA TCACGATGAA 
CACGACGACC ATGATGATCA TGAAGACGAA GACGAAGAGC CCCTCACAGA AGAGTTAGAA 
GAAGAACTGG AGGAAGAGGA AGAGCCAACT GAGGAGGATG AGCCAGCGGC AGATGAAGAA 
TATGAGGAGG ATGAAGATGA GGAGAACAAT GCGGGTGAAA ACATAACTGC CGAGGATGCG 
GAGGAGGAGG AAGAGGAGGA GGATAACGAT GATGAAGGCA CCGTAGAGGC AACTGTAGAA 
GCTACCACTG AAGCCACTAC GGAAGCCACT GGCGAATATG AAGCTGAAGA AGATGATGAG 
GACGAAGCAG CAGCAGATGA TGATGCAGTA GAGTCCACTG AAGCGCCGCT CTCGAAAGCT 
GAGGAGCAGG AAGATGATGA GGATGAGGAT GAGGAAGAGC AAGAGATCGA AGAATCAGTA 
GAGCCGGAAA GATCCCAATA TGCGGCACAA AGTGCTCCCG CCAAAGATAA TAATGACGAC 
GACGATGATG ATGATCAAGA CAAAAACGAT GCAGATGATG GGGATGACGA CGAATTTGAG 
TCCCTGGCTC AGCAATTCGA AAATGATCCC GAGGATACAG TGCCAGCAAA AGCTGTAGAG 
AGTGAGGAGC AGGATCAAGA CCAGGCTGAT GAGGAGGAGC CCAAGGAAGA GGGCTCCTCT 
TGGTTGGCAT CACTTGCCGT TAAATTTGGC GTTGGCGTTG CACTTGCCTT AGTTTCACGC 
CTTGTATTGA TAAGGAAAAG TCCAAATACA ACTGAGGATG AGCCCGCACC AGAGACTATA 
TTCAGGCGAA GATTGACGAT TGCCACTGCT GAGGATCACA TACCGGATGA TGTGGAGGAG 
CTGCCCCCTT TGGACGATGA ATATTCCGAG GAAGAAATCG AAATCGAAGA GGAAATCGAG 
GTGGAAATCA GTGATATTGA GGAGGAGGAG GAGGAAGTAC GTCACGATAA CGACGACAAC 
GTGGCCTCCA TGGCCAGTTA TGTGCCCGAG ACATTTGAGC AACTGAGCGC CATGTACAAA 
TACGCCCAGG AGCCTACGAA GGATGCCAAA CCCGAACAGA AAGAAAAGGA GCCGGAAAAG 
CCAGTAGGTC ACAGCGATAT CTACGTGGAA TACGAAGATG GTGCTATCGA GGATTACGAG 
CATGACGGAG TTAGCGATGA GGAGATCACC GACGAGGAGG ACGAAATCTC CGACGTGGAC 
GACGCCGATC TGATGAACCG GCTGGAAGCG AAATACGGTC GTCTGCCCGT CAAGGAGTTT 
GAGAGCGATC CGGACTCTGA CGACCCCAGC TGGACACGTA ATTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246358.1
CDS	119..1846
Translation	MSGDVQPRKRKDKRRKRDDRKSEGDVTVLRQSSFQDDDESSHGVHITKMGNEDLHLHVHHDHGTGGHWCAKIIFFALMAVLLGLVGLIIMENRGLEDLDTPLSESRFSKVFDGWVDEHRDEHDGHDVQEPSGEALDDHDEHDDHDDHEDEDEEPLTEELEEELEEEEEPTEEDEPAADEEYEEDEDEENNAGENITAEDAEEEEEEEDNDDEGTVEATVEATTEATTEATGEYEAEEDDEDEAAADDDAVESTEAPLSKAEEQEDDEDEDEEEQEIEESVEPERSQYAAQSAPAKDNNDDDDDDDQDKNDADDGDDDEFESLAQQFENDPEDTVPAKAVESEEQDQDQADEEEPKEEGSSWLASLAVKFGVGVALALVSRLVLIRKSPNTTEDEPAPETIFRRRLTIATAEDHIPDDVEELPPLDDEYSEEEIEIEEEIEVEISDIEEEEEEVRHDNDDNVASMASYVPETFEQLSAMYKYAQEPTKDAKPEQKEKEPEKPVGHSDIYVEYEDGAIEDYEHDGVSDEEITDEEDEISDVDDADLMNRLEAKYGRLPVKEFESDPDSDDPSWTRNY

Target ORF information:

RefSeq Version	NM_001259429.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster aspartyl beta-hydroxylase, transcript variant I (Asph), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259429.2

ORF Insert Sequence:

ATGTCGGGCG ATGTGCAGCC GCGTAAGCGC AAGGATAAGC GCCGAAAACG CGATGATCGC 
AAGTCCGAGG GCGATGTGAC TGTTCTGCGT CAGTCCAGTT TTCAAGATGA CGACGAATCT 
TCGCACGGCG TTCACATTAC AAAGATGGGA AACGAAGATC TCCACCTGCA TGTGCACCAC 
GATCACGGAA CTGGCGGTCA CTGGTGCGCC AAGATTATAT TCTTCGCCCT GATGGCAGTG 
CTCCTCGGTT TGGTGGGTCT GATCATCATG GAGAATCGCG GACTGGAGGA TCTGGACACT 
CCTCTGTCGG AGTCTAGGTT CTCGAAAGTT TTTGATGGCT GGGTGGACGA ACATCGAGAT 
GAGCATGATG GTCACGATGT GCAGGAACCC TCGGGAGAGG CGTTAGATGA TCACGATGAA 
CACGACGACC ATGATGATCA TGAAGACGAA GACGAAGAGC CCCTCACAGA AGAGTTAGAA 
GAAGAACTGG AGGAAGAGGA AGAGCCAACT GAGGAGGATG AGCCAGCGGC AGATGAAGAA 
TATGAGGAGG ATGAAGATGA GGAGAACAAT GCGGGTGAAA ACATAACTGC CGAGGATGCG 
GAGGAGGAGG AAGAGGAGGA GGATAACGAT GATGAAGGCA CCGTAGAGGC AACTGTAGAA 
GCTACCACTG AAGCCACTAC GGAAGCCACT GGCGAATATG AAGCTGAAGA AGATGATGAG 
GACGAAGCAG CAGCAGATGA TGATGCAGTA GAGTCCACTG AAGCGCCGCT CTCGAAAGCT 
GAGGAGCAGG AAGATGATGA GGATGAGGAT GAGGAAGAGC AAGAGATCGA AGAATCAGTA 
GAGCCGGAAA GATCCCAATA TGCGGCACAA AGTGCTCCCG CCAAAGATAA TAATGACGAC 
GACGATGATG ATGATCAAGA CAAAAACGAT GCAGATGATG GGGATGACGA CGAATTTGAG 
TCCCTGGCTC AGCAATTCGA AAATGATCCC GAGGATACAG TGCCAGCAAA AGCTGTAGAG 
AGTGAGGAGC AGGATCAAGA CCAGGCTGAT GAGGAGGAGC CCAAGGAAGA GGGCTCCTCT 
TGGTTGGCAT CACTTGCCGT TAAATTTGGC GTTGGCGTTG CACTTGCCTT AGTTTCACGC 
CTTGTATTGA TAAGGAAAAG TCCAAATACA ACTGAGGATG AGCCCGCACC AGAGACTATA 
TTCAGGCGAA GATTGACGAT TGCCACTGCT GAGGATCACA TACCGGATGA TGTGGAGGAG 
CTGCCCCCTT TGGACGATGA ATATTCCGAG GAAGAAATCG AAATCGAAGA GGAAATCGAG 
GTGGAAATCA GTGATATTGA GGAGGAGGAG GAGGAAGTAC GTCACGATAA CGACGACAAC 
GTGGCCTCCA TGGCCAGTTA TGTGCCCGAG ACATTTGAGC AACTGAGCGC CATGTACAAA 
TACGCCCAGG AGCCTACGAA GGATGCCAAA CCCGAACAGA AAGAAAAGGA GCCGGAAAAG 
CCAGTAGGTC ACAGCGATAT CTACGTGGAA TACGAAGATG GTGCTATCGA GGATTACGAG 
CATGACGGAG TTAGCGATGA GGAGATCACC GACGAGGAGG ACGAAATCTC CGACGTGGAC 
GACGCCGATC TGATGAACCG GCTGGAAGCG AAATACGGTC GTCTGCCCGT CAAGGAGTTT 
GAGAGCGATC CGGACTCTGA CGACCCCAGC TGGACACGTA ATTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asph ( NM_001259430.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246359.1 Drosophila melanogaster aspartyl beta-hydroxylase, transcript variant J (Asph), mRNA.

Price & Availability↑

CloneID	OFa12295
Clone ID Related Accession (Same CDS sequence)	NM_001259430.2
Accession Version	NM_001259430.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	aspartyl beta-hydroxylase, isoform J
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001259430.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGGCG ATGTGCAGCC GCGTAAGCGC AAGGATAAGC GCCGAAAACG CGATGATCGC 
AAGTCCGAGG GCGATGTGAC TGTTCTGCGT CAGTCCAGTT TTCAAGATGA CGACGAATCT 
TCGCACGGCG TTCACATTAC AAAGATGGGA AACGAAGATC TCCACCTGCA TGTGCACCAC 
GATCACGGAA CTGGCGGTCA CTGGTGCGCC AAGATTATAT TCTTCGCCCT GATGGCAGTG 
CTCCTCGGTT TGGTGGGTCT GATCATCATG GAGAATCGCG GACTGGAGGA TCTGGACACT 
CCTCTGTCGG AGTCTAGGTT CTCGAAAGTT TTTGATGGCT GGGTGGACGA ACATCGAGAT 
GAGCATGATG GTCACGATGT GCAGGAACCC TCGGGAGAGG CGTTAGATGA TCACGATGAA 
CACGACGACC ATGATGATCA TGAAGACGAA GACGAAGAGC CCCTCACAGA AGAGTTAGAA 
GAAGAACTGG AGGAAGAGGA AGAGCCAACT GAGGAGGATG AGCCAGCGGC AGATGAAGAA 
TATGAGGAGG ATGAAGATGA GGAGAACAAT GCGGGTGAAA ACATAACTGC CGAGGATGCG 
GAGGAGGAGG AAGAGGAGGA GGATAACGAT GATGAAGGCA CCGTAGAGGC AACTGTAGAA 
GCTACCACTG AAGCCACTAC GGAAGCCACT GGCGAATATG AAGCTGAAGA AGATGATGAG 
GACGAAGCAG CAGCAGATGA TGATGCAGTA GAGTCCACTG AAGCGCCGCT CTCGAAAGCT 
GAGGAGCAGG AAGATGATGA GGATGAGGAT GAGGAAGAGC AAGAGATCGA AGAATCAGTA 
GAGCCGGAAA GATCCCAATA TGCGGCACAA AGTGCTCCCG CCAAAGATAA TAATGACGAC 
GACGATGATG ATGATCAAGA CAAAAACGAT GCAGATGATG GGGATGACGA CGAATTTGAG 
TCCCTGGCTC AGCAATTCGA AAATGATCCC GAGGATACAG TGCCAGCAAA AGCTGTAGAG 
AGTGAGGAGC AGGATCAAGA CCAGGCTGAT GAGGAGGAGC CCAAGGAAGA GGGCTCCTCT 
TGGTTGGCAT CACAAATAAA GCCGAAAGAA GCTGCACCTG CTCCCGCAGA GAAAGAGGAT 
CCTTTCGAGC AAGAGTTGCG CAAGGCAAAC GAGGAGATGA TTAGGGAGCT CAACAATCAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246359.1
CDS	119..1321
Translation	MSGDVQPRKRKDKRRKRDDRKSEGDVTVLRQSSFQDDDESSHGVHITKMGNEDLHLHVHHDHGTGGHWCAKIIFFALMAVLLGLVGLIIMENRGLEDLDTPLSESRFSKVFDGWVDEHRDEHDGHDVQEPSGEALDDHDEHDDHDDHEDEDEEPLTEELEEELEEEEEPTEEDEPAADEEYEEDEDEENNAGENITAEDAEEEEEEEDNDDEGTVEATVEATTEATTEATGEYEAEEDDEDEAAADDDAVESTEAPLSKAEEQEDDEDEDEEEQEIEESVEPERSQYAAQSAPAKDNNDDDDDDDQDKNDADDGDDDEFESLAQQFENDPEDTVPAKAVESEEQDQDQADEEEPKEEGSSWLASQIKPKEAAPAPAEKEDPFEQELRKANEEMIRELNNH

Target ORF information:

RefSeq Version	NM_001259430.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster aspartyl beta-hydroxylase, transcript variant J (Asph), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259430.2

ORF Insert Sequence:

ATGTCGGGCG ATGTGCAGCC GCGTAAGCGC AAGGATAAGC GCCGAAAACG CGATGATCGC 
AAGTCCGAGG GCGATGTGAC TGTTCTGCGT CAGTCCAGTT TTCAAGATGA CGACGAATCT 
TCGCACGGCG TTCACATTAC AAAGATGGGA AACGAAGATC TCCACCTGCA TGTGCACCAC 
GATCACGGAA CTGGCGGTCA CTGGTGCGCC AAGATTATAT TCTTCGCCCT GATGGCAGTG 
CTCCTCGGTT TGGTGGGTCT GATCATCATG GAGAATCGCG GACTGGAGGA TCTGGACACT 
CCTCTGTCGG AGTCTAGGTT CTCGAAAGTT TTTGATGGCT GGGTGGACGA ACATCGAGAT 
GAGCATGATG GTCACGATGT GCAGGAACCC TCGGGAGAGG CGTTAGATGA TCACGATGAA 
CACGACGACC ATGATGATCA TGAAGACGAA GACGAAGAGC CCCTCACAGA AGAGTTAGAA 
GAAGAACTGG AGGAAGAGGA AGAGCCAACT GAGGAGGATG AGCCAGCGGC AGATGAAGAA 
TATGAGGAGG ATGAAGATGA GGAGAACAAT GCGGGTGAAA ACATAACTGC CGAGGATGCG 
GAGGAGGAGG AAGAGGAGGA GGATAACGAT GATGAAGGCA CCGTAGAGGC AACTGTAGAA 
GCTACCACTG AAGCCACTAC GGAAGCCACT GGCGAATATG AAGCTGAAGA AGATGATGAG 
GACGAAGCAG CAGCAGATGA TGATGCAGTA GAGTCCACTG AAGCGCCGCT CTCGAAAGCT 
GAGGAGCAGG AAGATGATGA GGATGAGGAT GAGGAAGAGC AAGAGATCGA AGAATCAGTA 
GAGCCGGAAA GATCCCAATA TGCGGCACAA AGTGCTCCCG CCAAAGATAA TAATGACGAC 
GACGATGATG ATGATCAAGA CAAAAACGAT GCAGATGATG GGGATGACGA CGAATTTGAG 
TCCCTGGCTC AGCAATTCGA AAATGATCCC GAGGATACAG TGCCAGCAAA AGCTGTAGAG 
AGTGAGGAGC AGGATCAAGA CCAGGCTGAT GAGGAGGAGC CCAAGGAAGA GGGCTCCTCT 
TGGTTGGCAT CACAAATAAA GCCGAAAGAA GCTGCACCTG CTCCCGCAGA GAAAGAGGAT 
CCTTTCGAGC AAGAGTTGCG CAAGGCAAAC GAGGAGATGA TTAGGGAGCT CAACAATCAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Asph ( NM_001259428.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246357.1 Drosophila melanogaster aspartyl beta-hydroxylase, transcript variant H (Asph), mRNA.

Price & Availability↑

CloneID	OFa12296
Clone ID Related Accession (Same CDS sequence)	NM_001259428.2
Accession Version	NM_001259428.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	aspartyl beta-hydroxylase, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001259428.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGGCG ATGTGCAGCC GCGTAAGCGC AAGGATAAGC GCCGAAAACG CGATGATCGC 
AAGTCCGAGG GCGATGTGAC TGTTCTGCGT CAGTCCAGTT TTCAAGATGA CGACGAATCT 
TCGCACGGCG TTCACATTAC AAAGATGGGA AACGAAGATC TCCACCTGCA TGTGCACCAC 
GATCACGGAA CTGGCGGTCA CTGGTGCGCC AAGATTATAT TCTTCGCCCT GATGGCAGTG 
CTCCTCGGTT TGGTGGGTCT GATCATCATG GAGAATCGCG GACTGGAGGA TCTGGACACT 
CCTCTGTCGG AGTCTAGGTT CTCGAAAGTT TTTGATGGCT GGGTGGACGA ACATCGAGAT 
GAGCATGATG GTCACGATGT GCAGGAACCC TCGGGAGAGG CGTTAGATGA TCACGATGAA 
CACGACGACC ATGATGATCA TGAAGACGAA GACGAAGAGC CCCTCACAGA AGAGTTAGAA 
GAAGAACTGG AGGAAGAGGA AGAGCCAACT GAGGAGGATG AGCCAGCGGC AGATGAAGAA 
TATGAGGAGG ATGAAGATGA GGAGAACAAT GCGGGTGAAA ACATAACTGC CGAGGATGCG 
GAGGAGGAGG AAGAGGAGGA GGATAACGAT GATGAAGGCA CCGTAGAGGC AACTGTAGAA 
GCTACCACTG AAGCCACTAC GGAAGCCACT GGCGAATATG AAGCTGAAGA AGATGATGAG 
GACGAAGCAG CAGCAGATGA TGATGCAGTA GAGTCCACTG AAGCGCCGCT CTCGAAAGCT 
GAGGAGCAGG AAGATGATGA GGATGAGGAT GAGGAAGAGC AAGAGATCGA AGAATCAGTA 
GAGCCGGAAA GATCCCAATA TGCGGCACAA AGTGCTCCCG CCAAAGATAA TAATGACGAC 
GACGATGATG ATGATCAAGA CAAAAACGAT GCAGATGATG GGGATGACGA CGAATTTGAG 
TCCCTGGCTC AGCAATTCGA AAATGATCCC GAGGATACAG TGCCAGCAAA AGCTGTAGAG 
AGTGAGGAGC AGGATCAAGA CCAGGCTGAT GAGGAGGAGC CCAAGGAAGA GGGCTCCTCT 
TGGTTGGCAT CACAAATAAA GCCGAAAGAA GCTGCACCTG CTCCCGCAGA GAAAGAGGAT 
CCTTTCGAGC AAGAGTTGCG CAAGGCAAAC GAGGAGATGA TTAGGGAGAA CTATGCCCAA 
GCCCTGCGTT CCTTTAACAC GCTCACCACC AACTTTGCCC ACGAGCCATC GGCCCATTTG 
GGAAGAGCTC GACTTCTGGA ACTACTGGCC AAAAAGGAGC GCAGCAATCA GCGCCTGTGG 
GAAGCCATCG ATGCTTACAA AAGATATTTG GCCTTTGGCG AGCTTGTAGC CAGCAATCAG 
GAATTTCAAA CCGCCGGCGA AAGCTGCATC GAGAATTTGA GATTTTTGGG TCACCACCGA 
CAGGCAACTA CCATCCATGA GCTGCTCATC AATCGTTTGC CTGAGGATCC ACGGCTTCGT 
AACCAACTAT CACTCACCTA TCTTATGGTA AACAATCTTC AGCAGGTTGA AAAGGTGGCT 
GTGGAGACCC TGAAACTTTG GCCCAACAAT GCAGTGGCTC AACTTCATTA TGGACTAGCA 
CTCAGACAGT TCCATGCTGA TTACGCCAAG GCGCTGCCTT ATCTAAAATA TGCCGTGGAG 
TCTGGAGAGG AGGGCACGCA AGAGGCTTTC TTCTATTTGT CGCTGGGCGA GACCATGCAG 
CGCCTGTCCA TGAAATCAGA AGCTCTTGAG GTGTACGGTA AAGGTGTTGC CAAAGGATTC 
TTTGCCAGCC TCTATCAGAG ATCGCTGTAC AATGAGCCCA GGTTGAGAGC ACAGCCATTT 
TGGCAGCCCA AGGAAACGGG CTATGAAAGG CAGCTGGAGA AGCTGACGCT CAATTGGCGT 
GCCATTCGCG ATGAAGGACT AGCACTGCTG GGAAGAAGTG GATTCTTCGA GGATGAAGCG 
GAACTGCTGC GCGACAAAGG AGTGTGGCAA CAGTATGAGC TATATGCCCA GGGTCGTCGG 
GTGAAGGACA ACTGCCGCAG GGCCCCAATC ACCTGTAGCC TGCTGGAGGA ATTTCCCGAG 
TCCGCCGGCT GTCGACGTGG CCAAGTGAAG TTTAGTGTTA TGCAGGCCAA GACGCATGTG 
TGGCCGCATT GTGGACCCAC CAATTGCCGA CTGAGGGCCC ACCTCACGCT AGCTGCTCCG 
GAGCCGGAAA AAGCATCACT CCGCGTGGCG GAGCAGGAAA GAACCTGGCG TGAGGGAGAA 
CTGTTCATAT TTGATGATAG CTTCGAACAC GAGGTGTGGC ACAACGGAAG CCAGTCACGC 
CTTGTGCTCA TCCTAGATAT GTGGCATCCG CAACTGAGTG CCGCCCAGCG CCGCAGTTTA 
TCACCCATTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246357.1
CDS	119..2530
Translation	MSGDVQPRKRKDKRRKRDDRKSEGDVTVLRQSSFQDDDESSHGVHITKMGNEDLHLHVHHDHGTGGHWCAKIIFFALMAVLLGLVGLIIMENRGLEDLDTPLSESRFSKVFDGWVDEHRDEHDGHDVQEPSGEALDDHDEHDDHDDHEDEDEEPLTEELEEELEEEEEPTEEDEPAADEEYEEDEDEENNAGENITAEDAEEEEEEEDNDDEGTVEATVEATTEATTEATGEYEAEEDDEDEAAADDDAVESTEAPLSKAEEQEDDEDEDEEEQEIEESVEPERSQYAAQSAPAKDNNDDDDDDDQDKNDADDGDDDEFESLAQQFENDPEDTVPAKAVESEEQDQDQADEEEPKEEGSSWLASQIKPKEAAPAPAEKEDPFEQELRKANEEMIRENYAQALRSFNTLTTNFAHEPSAHLGRARLLELLAKKERSNQRLWEAIDAYKRYLAFGELVASNQEFQTAGESCIENLRFLGHHRQATTIHELLINRLPEDPRLRNQLSLTYLMVNNLQQVEKVAVETLKLWPNNAVAQLHYGLALRQFHADYAKALPYLKYAVESGEEGTQEAFFYLSLGETMQRLSMKSEALEVYGKGVAKGFFASLYQRSLYNEPRLRAQPFWQPKETGYERQLEKLTLNWRAIRDEGLALLGRSGFFEDEAELLRDKGVWQQYELYAQGRRVKDNCRRAPITCSLLEEFPESAGCRRGQVKFSVMQAKTHVWPHCGPTNCRLRAHLTLAAPEPEKASLRVAEQERTWREGELFIFDDSFEHEVWHNGSQSRLVLILDMWHPQLSAAQRRSLSPI

Target ORF information:

RefSeq Version	NM_001259428.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster aspartyl beta-hydroxylase, transcript variant H (Asph), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259428.2

ORF Insert Sequence:

ATGTCGGGCG ATGTGCAGCC GCGTAAGCGC AAGGATAAGC GCCGAAAACG CGATGATCGC 
AAGTCCGAGG GCGATGTGAC TGTTCTGCGT CAGTCCAGTT TTCAAGATGA CGACGAATCT 
TCGCACGGCG TTCACATTAC AAAGATGGGA AACGAAGATC TCCACCTGCA TGTGCACCAC 
GATCACGGAA CTGGCGGTCA CTGGTGCGCC AAGATTATAT TCTTCGCCCT GATGGCAGTG 
CTCCTCGGTT TGGTGGGTCT GATCATCATG GAGAATCGCG GACTGGAGGA TCTGGACACT 
CCTCTGTCGG AGTCTAGGTT CTCGAAAGTT TTTGATGGCT GGGTGGACGA ACATCGAGAT 
GAGCATGATG GTCACGATGT GCAGGAACCC TCGGGAGAGG CGTTAGATGA TCACGATGAA 
CACGACGACC ATGATGATCA TGAAGACGAA GACGAAGAGC CCCTCACAGA AGAGTTAGAA 
GAAGAACTGG AGGAAGAGGA AGAGCCAACT GAGGAGGATG AGCCAGCGGC AGATGAAGAA 
TATGAGGAGG ATGAAGATGA GGAGAACAAT GCGGGTGAAA ACATAACTGC CGAGGATGCG 
GAGGAGGAGG AAGAGGAGGA GGATAACGAT GATGAAGGCA CCGTAGAGGC AACTGTAGAA 
GCTACCACTG AAGCCACTAC GGAAGCCACT GGCGAATATG AAGCTGAAGA AGATGATGAG 
GACGAAGCAG CAGCAGATGA TGATGCAGTA GAGTCCACTG AAGCGCCGCT CTCGAAAGCT 
GAGGAGCAGG AAGATGATGA GGATGAGGAT GAGGAAGAGC AAGAGATCGA AGAATCAGTA 
GAGCCGGAAA GATCCCAATA TGCGGCACAA AGTGCTCCCG CCAAAGATAA TAATGACGAC 
GACGATGATG ATGATCAAGA CAAAAACGAT GCAGATGATG GGGATGACGA CGAATTTGAG 
TCCCTGGCTC AGCAATTCGA AAATGATCCC GAGGATACAG TGCCAGCAAA AGCTGTAGAG 
AGTGAGGAGC AGGATCAAGA CCAGGCTGAT GAGGAGGAGC CCAAGGAAGA GGGCTCCTCT 
TGGTTGGCAT CACAAATAAA GCCGAAAGAA GCTGCACCTG CTCCCGCAGA GAAAGAGGAT 
CCTTTCGAGC AAGAGTTGCG CAAGGCAAAC GAGGAGATGA TTAGGGAGAA CTATGCCCAA 
GCCCTGCGTT CCTTTAACAC GCTCACCACC AACTTTGCCC ACGAGCCATC GGCCCATTTG 
GGAAGAGCTC GACTTCTGGA ACTACTGGCC AAAAAGGAGC GCAGCAATCA GCGCCTGTGG 
GAAGCCATCG ATGCTTACAA AAGATATTTG GCCTTTGGCG AGCTTGTAGC CAGCAATCAG 
GAATTTCAAA CCGCCGGCGA AAGCTGCATC GAGAATTTGA GATTTTTGGG TCACCACCGA 
CAGGCAACTA CCATCCATGA GCTGCTCATC AATCGTTTGC CTGAGGATCC ACGGCTTCGT 
AACCAACTAT CACTCACCTA TCTTATGGTA AACAATCTTC AGCAGGTTGA AAAGGTGGCT 
GTGGAGACCC TGAAACTTTG GCCCAACAAT GCAGTGGCTC AACTTCATTA TGGACTAGCA 
CTCAGACAGT TCCATGCTGA TTACGCCAAG GCGCTGCCTT ATCTAAAATA TGCCGTGGAG 
TCTGGAGAGG AGGGCACGCA AGAGGCTTTC TTCTATTTGT CGCTGGGCGA GACCATGCAG 
CGCCTGTCCA TGAAATCAGA AGCTCTTGAG GTGTACGGTA AAGGTGTTGC CAAAGGATTC 
TTTGCCAGCC TCTATCAGAG ATCGCTGTAC AATGAGCCCA GGTTGAGAGC ACAGCCATTT 
TGGCAGCCCA AGGAAACGGG CTATGAAAGG CAGCTGGAGA AGCTGACGCT CAATTGGCGT 
GCCATTCGCG ATGAAGGACT AGCACTGCTG GGAAGAAGTG GATTCTTCGA GGATGAAGCG 
GAACTGCTGC GCGACAAAGG AGTGTGGCAA CAGTATGAGC TATATGCCCA GGGTCGTCGG 
GTGAAGGACA ACTGCCGCAG GGCCCCAATC ACCTGTAGCC TGCTGGAGGA ATTTCCCGAG 
TCCGCCGGCT GTCGACGTGG CCAAGTGAAG TTTAGTGTTA TGCAGGCCAA GACGCATGTG 
TGGCCGCATT GTGGACCCAC CAATTGCCGA CTGAGGGCCC ACCTCACGCT AGCTGCTCCG 
GAGCCGGAAA AAGCATCACT CCGCGTGGCG GAGCAGGAAA GAACCTGGCG TGAGGGAGAA 
CTGTTCATAT TTGATGATAG CTTCGAACAC GAGGTGTGGC ACAACGGAAG CCAGTCACGC 
CTTGTGCTCA TCCTAGATAT GTGGCATCCG CAACTGAGTG CCGCCCAGCG CCGCAGTTTA 
TCACCCATTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Asph cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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