CG12964 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG12964
Entrez Gene ID	36714
Full Name	CG12964 gene product from transcript CG12964-RB
Synonyms	Dmel\CG12964
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 52A4-52A6

mRNA and Protein(s)

mRNA	Protein	Name
NM_137196.3	NP_611040.2	uncharacterized protein, isoform B
NM_001299531.1	NP_001286460.1	uncharacterized protein, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG12964 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG12964 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10092	NM_001299531.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant C (CG12964), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa10092	NM_137196.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG12964), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG12964 ( NM_137196.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611040.2 Drosophila melanogaster uncharacterized protein, transcript variant B (CG12964), mRNA.

Price & Availability↑

CloneID	OFa10092
Clone ID Related Accession (Same CDS sequence)	NM_137196.3 , NM_001299531.1
Accession Version	NM_137196.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137196.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGAACTC TGCCATTTTT GTTGGCGCTG GCGCTCTTGG CCTTTGGAGT TGCCTCATCA 
TCATCCTCCA CCACCAGCAC CACCACATCG GTAAAGCCAA AAACCGCAAA TCCAAAGGCT 
GAGTCCGATA AACCCACCAA GCAGCCATCG CCATCCGCAT CCCCATCGCC ATCATCCAAG 
GCCACAAACA CCTCAACGAC CCGGCAATCT AAAGCCTTGA AGCCGGGCGA GAATCGTGGC 
AAGAGAACGC TTTATGACTT TGGAAATGCC GGTTATCTGT ATCCCGAAGT GGCATCCCGG 
AGGGCTGGCT ACGTGGACAA CGCAGCGAGC TATTATCCCC AAACTGGTTA CTACAATGGT 
CAGAGTGCAG TTGCTCAATA TCCCGGAAGC TTTGGACCCG CCTATGCTCA ATATCCTCAG 
TATTTGGAGG CTCCAGAGCC CATCATCGAG ATCATCATCA AGGATGCGAA TGAAACATTA 
GCGGAAGAGC CCGCGCAGCC GATTGTGACC AAGAAGAAGA AGAAGAAGGA GAAGGTGCAC 
GTGTTCTATG TAAACTACAA GAAGGATCAG AACAACAAGT TGCACCTGGA GTCGCCAATT 
GCTTCGCTGA ACAACGACGA CAACGAGGAG GAAGAAGAGG AGGAGGAGGA AGTCATCCAG 
TATCCGGTTA CGCCCCTGCC TCCGGTAAAA TCCACCACCC TGCGTACTAT TATCCACCCG 
GACTCGGAGA AATACCACAG TAACTCCGGT ATTCATGTGT CGTTCGGAGC GGAGAACAAG 
CACCAGACCG GTCACATTCT GGAGGAGCAT GATGCTGAGA GCATTCAGCG CCAAGTGGTG 
GCACTGCCGC CTTCGGTGTC CTCCAAATCG GAGGTCAGCT ATGTGAGCAA TACCCGAGCG 
GATTACGGAA GAGAAAACAA CTTTGGATCC CGGCCAGGAC CTATTTCCAA TATGCTCTTT 
GGCAGCAACT ACCAACAGTC GGGTATCAAC TACCAGCAGC AACAACAGCA ACTGCCCACG 
CAGCACAGCA ACAACTACTT CAGGCCGCCA TCGGCTCTGG TGGGTCCTCC CCCCAACTAC 
CCGAAACCAG CGCCCCAGCA ACAGGTGCAG CAGCCCCATC GCCCCAGCAT CACCCAACAG 
CAATTGCCCT CCACTTCCTC CCAGACCATT TTCAACAAGC TGGTGCAACA GTCGCAGTTC 
TACATCAACC ACAATCAGCA ATACCCACAG CAGCAACATC AACAGCAGCA GCACCAGCAG 
CAACATCAAC AGCAGCAGCA TCAACAGCAG CAGCACCAAC AACAGCATCA GTATCAACAG 
CAGCATCAGC ATCAACATCA CCAGGCACCG CAGCAACATC AGCAACAGGC ACCGCAGCAG 
CAACAGTATC AGAAACCTCC GCATAAGCAA GTCCAGGTGC CATTCTTTCC AACCATTCCA 
CCTAAGAGCG TGACCCCTGC TCCCTACCAG CCCGTCAAGT TCCGCCCCAC ACCAGCACCT 
GTGGCCCCGG CCAAGCCCGT ACCTCTGCCC GTCAAACTGG ACAATGCGAA CTACCAACAG 
CAGCAGCAGC AACACTATCA ACCGCAGCAG CAACAGAAAT TGCCGCATCA ACAGCAATCT 
TATCAGTTCG TTCGTCAACC CCAATTTGTG CAGCAGCCAC AGTTTGTGGT GCGTTCACCC 
ACTCCACCCC CAGAATACTA CTCCCAGCAG AAGCAGCAGA CACAGCAGCA ACATCATCAG 
AGCGCTCTCA ATTATTTTGA CATCAAGCCA TCAAAGGAGA CGGAGCTGCT TAAGTCCATT 
CCCAAGTTCG AGCAGCACAT CACAGAGACC GTACAGAATC CAATTGCTAC CGCACAGCAG 
CCACAGCAGC AATATCATCA GCAGCAGGTG CAGCAACAAC AGCAGTTTGG ATATAGCAGC 
ACGAGGAATG AGGTGATCCA CGATGTACCT GCTCCCAACT TGGGACCCAC GGCTCAGCAG 
CACATCTCTG GGCACTATAT AACGGCAGGA TCATCTGGTG TCCAGCAGTC GCAGCAATTC 
GGCAGCTTTC CCAGTGCATC TCCAACGCAG CCTCCCGTCT ACGCAGAGTC CACTCATGGC 
CAGAAAGTAA TGGTAGTGAC TCCAGTGCCC CCATCCTCCA ACTATGTGAC CTATAGCCAG 
TACTCTCAGA CCGCTAATGA GCCTGTGGTC CACATTAATG CTCAGTCTGC GGCTCTGCAG 
GCTCAGGCCA GCACCAACTT CGCCCAACAG CCCAGGCAGC CCGCCTCCTC GCTGATCAGT 
GCTCCATCCA CGGATAATTC TCCCTTCAGT GTATCCACCT ACCACTCGGA CGTGTTCAAG 
GAGCTGGAGC AGCGCAACCA GAAGGATAAG AAGGTGTCAC AGCAGGTGCA GCAACAGGCT 
CAGCAAATTG CTGCAGCACC GGCTTCTGCT TCTGTTCCCG CTCCTACCTC CACTCCTGCC 
ACTGCTCCAA ATGGCAAGGC CTCACAGACC TTACTCCAGT TGCCCGACGA AGTGCCAGAT 
GATCTCCGCC AGCAACTTTT CTCCTCTGGT ATCCTGAACA ACGCCGATAT CTCAATTTTG 
GACTATGACA AGCAGGGGGA TATTGCCCTG GAGAACCTGC CAGCCGAGCA TTTGCAGCAC 
TTCTATGGAG CCGGTGGTGG TGCCCAGATA GCCGAGACCA ACAAGGTTTT GACTGTGGTC 
AAGCCCAATG GCGATAAGGT CGCCCTCAGC GAGAAGCAAC TGGATCGTGT GAAGCAGACC 
AACTCACTGC CCCAGAAACA GGATCTCGAT GTAAAGGTGG TGCGCTATGA TGCCGCTCAG 
GGTCAGAGTG TGAGCGATAA GTATGTGCGC ACCGATGCCA CCGTGGTGAC CCCGGTGGAT 
TTGGCCGAAC GGCAACAATA CAACCGCTAC CTCCCTCTGA AGATCAACGG AGCACAGTTC 
CCGATTCCCG ATAGCGAGGA GCTGCTAGGC AAGCGCATTG TCAGCGTGGT AGTCCTGGCT 
CCCGTGGAGG CCCAAAATCC TGGACAGGCG GCCGGTGAGG CGCGGAGCGC CGATGGCAAA 
GAGGTCAAGT TCCTGGGCGG TGAGCTCATC AAGACGCTGG TGAAGAAACC CACTAAAGAG 
AACTTTAAGC GCTGGCTGGA GAAGGAGGCG CGTACGGATT TGGAACTGCA GTCCGTCGTG 
CTGCTGGTGG CAAAATCCAC AGATCCATCC GCTGAGCAGG AGATCTTTAT GTACGAAATT 
GGAACGGGTA GCATAAACCG CCTCAACGGG GAGCTATCCA GCACGTTTGT AAACGTGGCC 
GAGGAGAACG CCAGCAGCGA GGATCTGGAG CACGCAGCCA CATTGGACCA TAGTTCGCTG 
GAGTCCATGA TGCATCTGCG CCGCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611040.2
CDS	160..3549
Translation	MRTLPFLLALALLAFGVASSSSSTTSTTTSVKPKTANPKAESDKPTKQPSPSASPSPSSKATNTSTTRQSKALKPGENRGKRTLYDFGNAGYLYPEVASRRAGYVDNAASYYPQTGYYNGQSAVAQYPGSFGPAYAQYPQYLEAPEPIIEIIIKDANETLAEEPAQPIVTKKKKKKEKVHVFYVNYKKDQNNKLHLESPIASLNNDDNEEEEEEEEEVIQYPVTPLPPVKSTTLRTIIHPDSEKYHSNSGIHVSFGAENKHQTGHILEEHDAESIQRQVVALPPSVSSKSEVSYVSNTRADYGRENNFGSRPGPISNMLFGSNYQQSGINYQQQQQQLPTQHSNNYFRPPSALVGPPPNYPKPAPQQQVQQPHRPSITQQQLPSTSSQTIFNKLVQQSQFYINHNQQYPQQQHQQQQHQQQHQQQQHQQQQHQQQHQYQQQHQHQHHQAPQQHQQQAPQQQQYQKPPHKQVQVPFFPTIPPKSVTPAPYQPVKFRPTPAPVAPAKPVPLPVKLDNANYQQQQQQHYQPQQQQKLPHQQQSYQFVRQPQFVQQPQFVVRSPTPPPEYYSQQKQQTQQQHHQSALNYFDIKPSKETELLKSIPKFEQHITETVQNPIATAQQPQQQYHQQQVQQQQQFGYSSTRNEVIHDVPAPNLGPTAQQHISGHYITAGSSGVQQSQQFGSFPSASPTQPPVYAESTHGQKVMVVTPVPPSSNYVTYSQYSQTANEPVVHINAQSAALQAQASTNFAQQPRQPASSLISAPSTDNSPFSVSTYHSDVFKELEQRNQKDKKVSQQVQQQAQQIAAAPASASVPAPTSTPATAPNGKASQTLLQLPDEVPDDLRQQLFSSGILNNADISILDYDKQGDIALENLPAEHLQHFYGAGGGAQIAETNKVLTVVKPNGDKVALSEKQLDRVKQTNSLPQKQDLDVKVVRYDAAQGQSVSDKYVRTDATVVTPVDLAERQQYNRYLPLKINGAQFPIPDSEELLGKRIVSVVVLAPVEAQNPGQAAGEARSADGKEVKFLGGELIKTLVKKPTKENFKRWLEKEARTDLELQSVVLLVAKSTDPSAEQEIFMYEIGTGSINRLNGELSSTFVNVAEENASSEDLEHAATLDHSSLESMMHLRRR

Target ORF information:

RefSeq Version	NM_137196.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG12964), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137196.3

ORF Insert Sequence:

ATGAGAACTC TGCCATTTTT GTTGGCGCTG GCGCTCTTGG CCTTTGGAGT TGCCTCATCA 
TCATCCTCCA CCACCAGCAC CACCACATCG GTAAAGCCAA AAACCGCAAA TCCAAAGGCT 
GAGTCCGATA AACCCACCAA GCAGCCATCG CCATCCGCAT CCCCATCGCC ATCATCCAAG 
GCCACAAACA CCTCAACGAC CCGGCAATCT AAAGCCTTGA AGCCGGGCGA GAATCGTGGC 
AAGAGAACGC TTTATGACTT TGGAAATGCC GGTTATCTGT ATCCCGAAGT GGCATCCCGG 
AGGGCTGGCT ACGTGGACAA CGCAGCGAGC TATTATCCCC AAACTGGTTA CTACAATGGT 
CAGAGTGCAG TTGCTCAATA TCCCGGAAGC TTTGGACCCG CCTATGCTCA ATATCCTCAG 
TATTTGGAGG CTCCAGAGCC CATCATCGAG ATCATCATCA AGGATGCGAA TGAAACATTA 
GCGGAAGAGC CCGCGCAGCC GATTGTGACC AAGAAGAAGA AGAAGAAGGA GAAGGTGCAC 
GTGTTCTATG TAAACTACAA GAAGGATCAG AACAACAAGT TGCACCTGGA GTCGCCAATT 
GCTTCGCTGA ACAACGACGA CAACGAGGAG GAAGAAGAGG AGGAGGAGGA AGTCATCCAG 
TATCCGGTTA CGCCCCTGCC TCCGGTAAAA TCCACCACCC TGCGTACTAT TATCCACCCG 
GACTCGGAGA AATACCACAG TAACTCCGGT ATTCATGTGT CGTTCGGAGC GGAGAACAAG 
CACCAGACCG GTCACATTCT GGAGGAGCAT GATGCTGAGA GCATTCAGCG CCAAGTGGTG 
GCACTGCCGC CTTCGGTGTC CTCCAAATCG GAGGTCAGCT ATGTGAGCAA TACCCGAGCG 
GATTACGGAA GAGAAAACAA CTTTGGATCC CGGCCAGGAC CTATTTCCAA TATGCTCTTT 
GGCAGCAACT ACCAACAGTC GGGTATCAAC TACCAGCAGC AACAACAGCA ACTGCCCACG 
CAGCACAGCA ACAACTACTT CAGGCCGCCA TCGGCTCTGG TGGGTCCTCC CCCCAACTAC 
CCGAAACCAG CGCCCCAGCA ACAGGTGCAG CAGCCCCATC GCCCCAGCAT CACCCAACAG 
CAATTGCCCT CCACTTCCTC CCAGACCATT TTCAACAAGC TGGTGCAACA GTCGCAGTTC 
TACATCAACC ACAATCAGCA ATACCCACAG CAGCAACATC AACAGCAGCA GCACCAGCAG 
CAACATCAAC AGCAGCAGCA TCAACAGCAG CAGCACCAAC AACAGCATCA GTATCAACAG 
CAGCATCAGC ATCAACATCA CCAGGCACCG CAGCAACATC AGCAACAGGC ACCGCAGCAG 
CAACAGTATC AGAAACCTCC GCATAAGCAA GTCCAGGTGC CATTCTTTCC AACCATTCCA 
CCTAAGAGCG TGACCCCTGC TCCCTACCAG CCCGTCAAGT TCCGCCCCAC ACCAGCACCT 
GTGGCCCCGG CCAAGCCCGT ACCTCTGCCC GTCAAACTGG ACAATGCGAA CTACCAACAG 
CAGCAGCAGC AACACTATCA ACCGCAGCAG CAACAGAAAT TGCCGCATCA ACAGCAATCT 
TATCAGTTCG TTCGTCAACC CCAATTTGTG CAGCAGCCAC AGTTTGTGGT GCGTTCACCC 
ACTCCACCCC CAGAATACTA CTCCCAGCAG AAGCAGCAGA CACAGCAGCA ACATCATCAG 
AGCGCTCTCA ATTATTTTGA CATCAAGCCA TCAAAGGAGA CGGAGCTGCT TAAGTCCATT 
CCCAAGTTCG AGCAGCACAT CACAGAGACC GTACAGAATC CAATTGCTAC CGCACAGCAG 
CCACAGCAGC AATATCATCA GCAGCAGGTG CAGCAACAAC AGCAGTTTGG ATATAGCAGC 
ACGAGGAATG AGGTGATCCA CGATGTACCT GCTCCCAACT TGGGACCCAC GGCTCAGCAG 
CACATCTCTG GGCACTATAT AACGGCAGGA TCATCTGGTG TCCAGCAGTC GCAGCAATTC 
GGCAGCTTTC CCAGTGCATC TCCAACGCAG CCTCCCGTCT ACGCAGAGTC CACTCATGGC 
CAGAAAGTAA TGGTAGTGAC TCCAGTGCCC CCATCCTCCA ACTATGTGAC CTATAGCCAG 
TACTCTCAGA CCGCTAATGA GCCTGTGGTC CACATTAATG CTCAGTCTGC GGCTCTGCAG 
GCTCAGGCCA GCACCAACTT CGCCCAACAG CCCAGGCAGC CCGCCTCCTC GCTGATCAGT 
GCTCCATCCA CGGATAATTC TCCCTTCAGT GTATCCACCT ACCACTCGGA CGTGTTCAAG 
GAGCTGGAGC AGCGCAACCA GAAGGATAAG AAGGTGTCAC AGCAGGTGCA GCAACAGGCT 
CAGCAAATTG CTGCAGCACC GGCTTCTGCT TCTGTTCCCG CTCCTACCTC CACTCCTGCC 
ACTGCTCCAA ATGGCAAGGC CTCACAGACC TTACTCCAGT TGCCCGACGA AGTGCCAGAT 
GATCTCCGCC AGCAACTTTT CTCCTCTGGT ATCCTGAACA ACGCCGATAT CTCAATTTTG 
GACTATGACA AGCAGGGGGA TATTGCCCTG GAGAACCTGC CAGCCGAGCA TTTGCAGCAC 
TTCTATGGAG CCGGTGGTGG TGCCCAGATA GCCGAGACCA ACAAGGTTTT GACTGTGGTC 
AAGCCCAATG GCGATAAGGT CGCCCTCAGC GAGAAGCAAC TGGATCGTGT GAAGCAGACC 
AACTCACTGC CCCAGAAACA GGATCTCGAT GTAAAGGTGG TGCGCTATGA TGCCGCTCAG 
GGTCAGAGTG TGAGCGATAA GTATGTGCGC ACCGATGCCA CCGTGGTGAC CCCGGTGGAT 
TTGGCCGAAC GGCAACAATA CAACCGCTAC CTCCCTCTGA AGATCAACGG AGCACAGTTC 
CCGATTCCCG ATAGCGAGGA GCTGCTAGGC AAGCGCATTG TCAGCGTGGT AGTCCTGGCT 
CCCGTGGAGG CCCAAAATCC TGGACAGGCG GCCGGTGAGG CGCGGAGCGC CGATGGCAAA 
GAGGTCAAGT TCCTGGGCGG TGAGCTCATC AAGACGCTGG TGAAGAAACC CACTAAAGAG 
AACTTTAAGC GCTGGCTGGA GAAGGAGGCG CGTACGGATT TGGAACTGCA GTCCGTCGTG 
CTGCTGGTGG CAAAATCCAC AGATCCATCC GCTGAGCAGG AGATCTTTAT GTACGAAATT 
GGAACGGGTA GCATAAACCG CCTCAACGGG GAGCTATCCA GCACGTTTGT AAACGTGGCC 
GAGGAGAACG CCAGCAGCGA GGATCTGGAG CACGCAGCCA CATTGGACCA TAGTTCGCTG 
GAGTCCATGA TGCATCTGCG CCGCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG12964 ( NM_001299531.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286460.1 Drosophila melanogaster uncharacterized protein, transcript variant C (CG12964), mRNA.

Price & Availability↑

CloneID	OFa10092
Clone ID Related Accession (Same CDS sequence)	NM_137196.3 , NM_001299531.1
Accession Version	NM_001299531.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGAACTC TGCCATTTTT GTTGGCGCTG GCGCTCTTGG CCTTTGGAGT TGCCTCATCA 
TCATCCTCCA CCACCAGCAC CACCACATCG GTAAAGCCAA AAACCGCAAA TCCAAAGGCT 
GAGTCCGATA AACCCACCAA GCAGCCATCG CCATCCGCAT CCCCATCGCC ATCATCCAAG 
GCCACAAACA CCTCAACGAC CCGGCAATCT AAAGCCTTGA AGCCGGGCGA GAATCGTGGC 
AAGAGAACGC TTTATGACTT TGGAAATGCC GGTTATCTGT ATCCCGAAGT GGCATCCCGG 
AGGGCTGGCT ACGTGGACAA CGCAGCGAGC TATTATCCCC AAACTGGTTA CTACAATGGT 
CAGAGTGCAG TTGCTCAATA TCCCGGAAGC TTTGGACCCG CCTATGCTCA ATATCCTCAG 
TATTTGGAGG CTCCAGAGCC CATCATCGAG ATCATCATCA AGGATGCGAA TGAAACATTA 
GCGGAAGAGC CCGCGCAGCC GATTGTGACC AAGAAGAAGA AGAAGAAGGA GAAGGTGCAC 
GTGTTCTATG TAAACTACAA GAAGGATCAG AACAACAAGT TGCACCTGGA GTCGCCAATT 
GCTTCGCTGA ACAACGACGA CAACGAGGAG GAAGAAGAGG AGGAGGAGGA AGTCATCCAG 
TATCCGGTTA CGCCCCTGCC TCCGGTAAAA TCCACCACCC TGCGTACTAT TATCCACCCG 
GACTCGGAGA AATACCACAG TAACTCCGGT ATTCATGTGT CGTTCGGAGC GGAGAACAAG 
CACCAGACCG GTCACATTCT GGAGGAGCAT GATGCTGAGA GCATTCAGCG CCAAGTGGTG 
GCACTGCCGC CTTCGGTGTC CTCCAAATCG GAGGTCAGCT ATGTGAGCAA TACCCGAGCG 
GATTACGGAA GAGAAAACAA CTTTGGATCC CGGCCAGGAC CTATTTCCAA TATGCTCTTT 
GGCAGCAACT ACCAACAGTC GGGTATCAAC TACCAGCAGC AACAACAGCA ACTGCCCACG 
CAGCACAGCA ACAACTACTT CAGGCCGCCA TCGGCTCTGG TGGGTCCTCC CCCCAACTAC 
CCGAAACCAG CGCCCCAGCA ACAGGTGCAG CAGCCCCATC GCCCCAGCAT CACCCAACAG 
CAATTGCCCT CCACTTCCTC CCAGACCATT TTCAACAAGC TGGTGCAACA GTCGCAGTTC 
TACATCAACC ACAATCAGCA ATACCCACAG CAGCAACATC AACAGCAGCA GCACCAGCAG 
CAACATCAAC AGCAGCAGCA TCAACAGCAG CAGCACCAAC AACAGCATCA GTATCAACAG 
CAGCATCAGC ATCAACATCA CCAGGCACCG CAGCAACATC AGCAACAGGC ACCGCAGCAG 
CAACAGTATC AGAAACCTCC GCATAAGCAA GTCCAGGTGC CATTCTTTCC AACCATTCCA 
CCTAAGAGCG TGACCCCTGC TCCCTACCAG CCCGTCAAGT TCCGCCCCAC ACCAGCACCT 
GTGGCCCCGG CCAAGCCCGT ACCTCTGCCC GTCAAACTGG ACAATGCGAA CTACCAACAG 
CAGCAGCAGC AACACTATCA ACCGCAGCAG CAACAGAAAT TGCCGCATCA ACAGCAATCT 
TATCAGTTCG TTCGTCAACC CCAATTTGTG CAGCAGCCAC AGTTTGTGGT GCGTTCACCC 
ACTCCACCCC CAGAATACTA CTCCCAGCAG AAGCAGCAGA CACAGCAGCA ACATCATCAG 
AGCGCTCTCA ATTATTTTGA CATCAAGCCA TCAAAGGAGA CGGAGCTGCT TAAGTCCATT 
CCCAAGTTCG AGCAGCACAT CACAGAGACC GTACAGAATC CAATTGCTAC CGCACAGCAG 
CCACAGCAGC AATATCATCA GCAGCAGGTG CAGCAACAAC AGCAGTTTGG ATATAGCAGC 
ACGAGGAATG AGGTGATCCA CGATGTACCT GCTCCCAACT TGGGACCCAC GGCTCAGCAG 
CACATCTCTG GGCACTATAT AACGGCAGGA TCATCTGGTG TCCAGCAGTC GCAGCAATTC 
GGCAGCTTTC CCAGTGCATC TCCAACGCAG CCTCCCGTCT ACGCAGAGTC CACTCATGGC 
CAGAAAGTAA TGGTAGTGAC TCCAGTGCCC CCATCCTCCA ACTATGTGAC CTATAGCCAG 
TACTCTCAGA CCGCTAATGA GCCTGTGGTC CACATTAATG CTCAGTCTGC GGCTCTGCAG 
GCTCAGGCCA GCACCAACTT CGCCCAACAG CCCAGGCAGC CCGCCTCCTC GCTGATCAGT 
GCTCCATCCA CGGATAATTC TCCCTTCAGT GTATCCACCT ACCACTCGGA CGTGTTCAAG 
GAGCTGGAGC AGCGCAACCA GAAGGATAAG AAGGTGTCAC AGCAGGTGCA GCAACAGGCT 
CAGCAAATTG CTGCAGCACC GGCTTCTGCT TCTGTTCCCG CTCCTACCTC CACTCCTGCC 
ACTGCTCCAA ATGGCAAGGC CTCACAGACC TTACTCCAGT TGCCCGACGA AGTGCCAGAT 
GATCTCCGCC AGCAACTTTT CTCCTCTGGT ATCCTGAACA ACGCCGATAT CTCAATTTTG 
GACTATGACA AGCAGGGGGA TATTGCCCTG GAGAACCTGC CAGCCGAGCA TTTGCAGCAC 
TTCTATGGAG CCGGTGGTGG TGCCCAGATA GCCGAGACCA ACAAGGTTTT GACTGTGGTC 
AAGCCCAATG GCGATAAGGT CGCCCTCAGC GAGAAGCAAC TGGATCGTGT GAAGCAGACC 
AACTCACTGC CCCAGAAACA GGATCTCGAT GTAAAGGTGG TGCGCTATGA TGCCGCTCAG 
GGTCAGAGTG TGAGCGATAA GTATGTGCGC ACCGATGCCA CCGTGGTGAC CCCGGTGGAT 
TTGGCCGAAC GGCAACAATA CAACCGCTAC CTCCCTCTGA AGATCAACGG AGCACAGTTC 
CCGATTCCCG ATAGCGAGGA GCTGCTAGGC AAGCGCATTG TCAGCGTGGT AGTCCTGGCT 
CCCGTGGAGG CCCAAAATCC TGGACAGGCG GCCGGTGAGG CGCGGAGCGC CGATGGCAAA 
GAGGTCAAGT TCCTGGGCGG TGAGCTCATC AAGACGCTGG TGAAGAAACC CACTAAAGAG 
AACTTTAAGC GCTGGCTGGA GAAGGAGGCG CGTACGGATT TGGAACTGCA GTCCGTCGTG 
CTGCTGGTGG CAAAATCCAC AGATCCATCC GCTGAGCAGG AGATCTTTAT GTACGAAATT 
GGAACGGGTA GCATAAACCG CCTCAACGGG GAGCTATCCA GCACGTTTGT AAACGTGGCC 
GAGGAGAACG CCAGCAGCGA GGATCTGGAG CACGCAGCCA CATTGGACCA TAGTTCGCTG 
GAGTCCATGA TGCATCTGCG CCGCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286460.1
CDS	245..3634
Translation	MRTLPFLLALALLAFGVASSSSSTTSTTTSVKPKTANPKAESDKPTKQPSPSASPSPSSKATNTSTTRQSKALKPGENRGKRTLYDFGNAGYLYPEVASRRAGYVDNAASYYPQTGYYNGQSAVAQYPGSFGPAYAQYPQYLEAPEPIIEIIIKDANETLAEEPAQPIVTKKKKKKEKVHVFYVNYKKDQNNKLHLESPIASLNNDDNEEEEEEEEEVIQYPVTPLPPVKSTTLRTIIHPDSEKYHSNSGIHVSFGAENKHQTGHILEEHDAESIQRQVVALPPSVSSKSEVSYVSNTRADYGRENNFGSRPGPISNMLFGSNYQQSGINYQQQQQQLPTQHSNNYFRPPSALVGPPPNYPKPAPQQQVQQPHRPSITQQQLPSTSSQTIFNKLVQQSQFYINHNQQYPQQQHQQQQHQQQHQQQQHQQQQHQQQHQYQQQHQHQHHQAPQQHQQQAPQQQQYQKPPHKQVQVPFFPTIPPKSVTPAPYQPVKFRPTPAPVAPAKPVPLPVKLDNANYQQQQQQHYQPQQQQKLPHQQQSYQFVRQPQFVQQPQFVVRSPTPPPEYYSQQKQQTQQQHHQSALNYFDIKPSKETELLKSIPKFEQHITETVQNPIATAQQPQQQYHQQQVQQQQQFGYSSTRNEVIHDVPAPNLGPTAQQHISGHYITAGSSGVQQSQQFGSFPSASPTQPPVYAESTHGQKVMVVTPVPPSSNYVTYSQYSQTANEPVVHINAQSAALQAQASTNFAQQPRQPASSLISAPSTDNSPFSVSTYHSDVFKELEQRNQKDKKVSQQVQQQAQQIAAAPASASVPAPTSTPATAPNGKASQTLLQLPDEVPDDLRQQLFSSGILNNADISILDYDKQGDIALENLPAEHLQHFYGAGGGAQIAETNKVLTVVKPNGDKVALSEKQLDRVKQTNSLPQKQDLDVKVVRYDAAQGQSVSDKYVRTDATVVTPVDLAERQQYNRYLPLKINGAQFPIPDSEELLGKRIVSVVVLAPVEAQNPGQAAGEARSADGKEVKFLGGELIKTLVKKPTKENFKRWLEKEARTDLELQSVVLLVAKSTDPSAEQEIFMYEIGTGSINRLNGELSSTFVNVAEENASSEDLEHAATLDHSSLESMMHLRRR

Target ORF information:

RefSeq Version	NM_001299531.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant C (CG12964), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299531.1

ORF Insert Sequence:

ATGAGAACTC TGCCATTTTT GTTGGCGCTG GCGCTCTTGG CCTTTGGAGT TGCCTCATCA 
TCATCCTCCA CCACCAGCAC CACCACATCG GTAAAGCCAA AAACCGCAAA TCCAAAGGCT 
GAGTCCGATA AACCCACCAA GCAGCCATCG CCATCCGCAT CCCCATCGCC ATCATCCAAG 
GCCACAAACA CCTCAACGAC CCGGCAATCT AAAGCCTTGA AGCCGGGCGA GAATCGTGGC 
AAGAGAACGC TTTATGACTT TGGAAATGCC GGTTATCTGT ATCCCGAAGT GGCATCCCGG 
AGGGCTGGCT ACGTGGACAA CGCAGCGAGC TATTATCCCC AAACTGGTTA CTACAATGGT 
CAGAGTGCAG TTGCTCAATA TCCCGGAAGC TTTGGACCCG CCTATGCTCA ATATCCTCAG 
TATTTGGAGG CTCCAGAGCC CATCATCGAG ATCATCATCA AGGATGCGAA TGAAACATTA 
GCGGAAGAGC CCGCGCAGCC GATTGTGACC AAGAAGAAGA AGAAGAAGGA GAAGGTGCAC 
GTGTTCTATG TAAACTACAA GAAGGATCAG AACAACAAGT TGCACCTGGA GTCGCCAATT 
GCTTCGCTGA ACAACGACGA CAACGAGGAG GAAGAAGAGG AGGAGGAGGA AGTCATCCAG 
TATCCGGTTA CGCCCCTGCC TCCGGTAAAA TCCACCACCC TGCGTACTAT TATCCACCCG 
GACTCGGAGA AATACCACAG TAACTCCGGT ATTCATGTGT CGTTCGGAGC GGAGAACAAG 
CACCAGACCG GTCACATTCT GGAGGAGCAT GATGCTGAGA GCATTCAGCG CCAAGTGGTG 
GCACTGCCGC CTTCGGTGTC CTCCAAATCG GAGGTCAGCT ATGTGAGCAA TACCCGAGCG 
GATTACGGAA GAGAAAACAA CTTTGGATCC CGGCCAGGAC CTATTTCCAA TATGCTCTTT 
GGCAGCAACT ACCAACAGTC GGGTATCAAC TACCAGCAGC AACAACAGCA ACTGCCCACG 
CAGCACAGCA ACAACTACTT CAGGCCGCCA TCGGCTCTGG TGGGTCCTCC CCCCAACTAC 
CCGAAACCAG CGCCCCAGCA ACAGGTGCAG CAGCCCCATC GCCCCAGCAT CACCCAACAG 
CAATTGCCCT CCACTTCCTC CCAGACCATT TTCAACAAGC TGGTGCAACA GTCGCAGTTC 
TACATCAACC ACAATCAGCA ATACCCACAG CAGCAACATC AACAGCAGCA GCACCAGCAG 
CAACATCAAC AGCAGCAGCA TCAACAGCAG CAGCACCAAC AACAGCATCA GTATCAACAG 
CAGCATCAGC ATCAACATCA CCAGGCACCG CAGCAACATC AGCAACAGGC ACCGCAGCAG 
CAACAGTATC AGAAACCTCC GCATAAGCAA GTCCAGGTGC CATTCTTTCC AACCATTCCA 
CCTAAGAGCG TGACCCCTGC TCCCTACCAG CCCGTCAAGT TCCGCCCCAC ACCAGCACCT 
GTGGCCCCGG CCAAGCCCGT ACCTCTGCCC GTCAAACTGG ACAATGCGAA CTACCAACAG 
CAGCAGCAGC AACACTATCA ACCGCAGCAG CAACAGAAAT TGCCGCATCA ACAGCAATCT 
TATCAGTTCG TTCGTCAACC CCAATTTGTG CAGCAGCCAC AGTTTGTGGT GCGTTCACCC 
ACTCCACCCC CAGAATACTA CTCCCAGCAG AAGCAGCAGA CACAGCAGCA ACATCATCAG 
AGCGCTCTCA ATTATTTTGA CATCAAGCCA TCAAAGGAGA CGGAGCTGCT TAAGTCCATT 
CCCAAGTTCG AGCAGCACAT CACAGAGACC GTACAGAATC CAATTGCTAC CGCACAGCAG 
CCACAGCAGC AATATCATCA GCAGCAGGTG CAGCAACAAC AGCAGTTTGG ATATAGCAGC 
ACGAGGAATG AGGTGATCCA CGATGTACCT GCTCCCAACT TGGGACCCAC GGCTCAGCAG 
CACATCTCTG GGCACTATAT AACGGCAGGA TCATCTGGTG TCCAGCAGTC GCAGCAATTC 
GGCAGCTTTC CCAGTGCATC TCCAACGCAG CCTCCCGTCT ACGCAGAGTC CACTCATGGC 
CAGAAAGTAA TGGTAGTGAC TCCAGTGCCC CCATCCTCCA ACTATGTGAC CTATAGCCAG 
TACTCTCAGA CCGCTAATGA GCCTGTGGTC CACATTAATG CTCAGTCTGC GGCTCTGCAG 
GCTCAGGCCA GCACCAACTT CGCCCAACAG CCCAGGCAGC CCGCCTCCTC GCTGATCAGT 
GCTCCATCCA CGGATAATTC TCCCTTCAGT GTATCCACCT ACCACTCGGA CGTGTTCAAG 
GAGCTGGAGC AGCGCAACCA GAAGGATAAG AAGGTGTCAC AGCAGGTGCA GCAACAGGCT 
CAGCAAATTG CTGCAGCACC GGCTTCTGCT TCTGTTCCCG CTCCTACCTC CACTCCTGCC 
ACTGCTCCAA ATGGCAAGGC CTCACAGACC TTACTCCAGT TGCCCGACGA AGTGCCAGAT 
GATCTCCGCC AGCAACTTTT CTCCTCTGGT ATCCTGAACA ACGCCGATAT CTCAATTTTG 
GACTATGACA AGCAGGGGGA TATTGCCCTG GAGAACCTGC CAGCCGAGCA TTTGCAGCAC 
TTCTATGGAG CCGGTGGTGG TGCCCAGATA GCCGAGACCA ACAAGGTTTT GACTGTGGTC 
AAGCCCAATG GCGATAAGGT CGCCCTCAGC GAGAAGCAAC TGGATCGTGT GAAGCAGACC 
AACTCACTGC CCCAGAAACA GGATCTCGAT GTAAAGGTGG TGCGCTATGA TGCCGCTCAG 
GGTCAGAGTG TGAGCGATAA GTATGTGCGC ACCGATGCCA CCGTGGTGAC CCCGGTGGAT 
TTGGCCGAAC GGCAACAATA CAACCGCTAC CTCCCTCTGA AGATCAACGG AGCACAGTTC 
CCGATTCCCG ATAGCGAGGA GCTGCTAGGC AAGCGCATTG TCAGCGTGGT AGTCCTGGCT 
CCCGTGGAGG CCCAAAATCC TGGACAGGCG GCCGGTGAGG CGCGGAGCGC CGATGGCAAA 
GAGGTCAAGT TCCTGGGCGG TGAGCTCATC AAGACGCTGG TGAAGAAACC CACTAAAGAG 
AACTTTAAGC GCTGGCTGGA GAAGGAGGCG CGTACGGATT TGGAACTGCA GTCCGTCGTG 
CTGCTGGTGG CAAAATCCAC AGATCCATCC GCTGAGCAGG AGATCTTTAT GTACGAAATT 
GGAACGGGTA GCATAAACCG CCTCAACGGG GAGCTATCCA GCACGTTTGT AAACGTGGCC 
GAGGAGAACG CCAGCAGCGA GGATCTGGAG CACGCAGCCA CATTGGACCA TAGTTCGCTG 
GAGTCCATGA TGCATCTGCG CCGCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG12964 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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