jef cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	jef
Entrez Gene ID	36634
Full Name	jet fuel
Synonyms	CG12858,Dmel\CG12858
General protein information	Preferred Names jet fuel Names CG12858 gene product from transcript CG12858-RB CG12858-PA CG12858-PB jef-PA jef-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 51C1-51C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001259394.2	NP_001246323.1	jet fuel, isoform B
NM_137139.2	NP_610983.1	jet fuel, isoform A

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	mfsd6a	NP_001076525.1
Homo sapiens (human)	MFSD6	NP_060164.3
Bos taurus (cattle)	MFSD6	XP_005202303.1
Gallus gallus (chicken)	MFSD6	XP_421837.1
	MFSD6	XP_001108155.1
Canis lupus familiaris (dog)	MFSD6	XP_005640516.1
Drosophila melanogaster (fruit fly)	CG12858	NP_001246323.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003204	XP_312908.4
Xenopus tropicalis (tropical clawed frog)	mfsd6	XP_002941640.1
Pan troglodytes (chimpanzee)	MFSD6	XP_001166645.1
Rattus norvegicus (Norway rat)	Mfsd6	NP_001100381.1
Caenorhabditis elegans (roundworm)	R13A5.9	NP_498662.1
Mus musculus (house mouse)	Mfsd6	NP_598590.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following jef gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the jef cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10007	NM_001259394.2 Latest version!	Drosophila melanogaster jet fuel (jef), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa10007	NM_137139.2 Latest version!	Drosophila melanogaster jet fuel (jef), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

jef ( NM_001259394.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246323.1 Drosophila melanogaster jet fuel (jef), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa10007
Clone ID Related Accession (Same CDS sequence)	NM_001259394.2 , NM_137139.2
Accession Version	NM_001259394.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	jet fuel, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001259394.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATCCCT ACACTGCAGG CGCCAGCGGA GGTGTCGGTG GCGGAGGAGG AGCCAATCCC 
TTCGGAGCAC CAGCCGGAGG TGCGGGATAT GGCCAGCAGG GCTATGGATA CGAGCAGCAG 
GCAACAGGTG GCTACGACCA GGGCATGAAC TACGGCATTG AGCCGCAGCA GCAGGCAGCC 
GGATACGGAC CACCTGGAGC CAGGCCACGG GTGGATGTGG AGGCCACTGG CGAGGTGGAT 
CCCAATCTAT ATCCAGAGGC GAAGGACTCA ACGCACAAAG TTCGCGGCCA CTCGGACATC 
CTGGAAATGT TTTTCGGGGC GAGCACAGAA CGGGAACTGA TACCCGTGAA AAGCTTCTAC 
TTTTTCTTCT ACGCTGCATT TGGATCACTG TTTCCGCTGA TGGGCGTGTA TTTCAAGCAG 
ATGGGCATGA ATCCGGGCCA GTGCGGAATT CTGGTGGGAA TGCGGCCATT CGTGGAGTTC 
CTGTCGGCCC CGTTTTGGGG CTCCTATGCG GATCGTTGTC GGCAGGGCAA GCGTCTGCTC 
CTGGGCTCCC TGGCCTGTTG GGTACTCTTT ACCATTCCGC TGAGCTTCAT CCGACCGGAG 
GCCATCAATT GCATTGAGCG ACGCAATGCC ACGGACTTTG TGCTGACCTA CACACGCACG 
AAGCGCGACC TGTCGGCCAT GTACGAGCAG GAGCACTTGG ATATGGGCAC TGGCACCATG 
CCAGCCGATG AGGCGCTGGA GGAGGCGGAG GCAGCACACA GTCGGCACAA GAGATCACTG 
CTGCTGCCAA GGATCGATGC GGGCATCTCA CCAGTGCACA TCAACTTTGT GAGCAACTAC 
GACGACAAGT ACCACAGGGA CTATGTGACG CCCATCTTTA GCTCCATGGT GTACAGGACT 
CCGGACATCC AAAAGGCGTT CTTCCTGCTG CTCCTGGTGA TCCTCATTGG GGAGTTCTTC 
AGCGCTCCGG CGATTACCTT GGCTGATTCC GCCGTAATAA CTTTGCTGGG CGAGGATGCG 
GACAAGTACG GTCACCAGCG AATGTTTGGG TCACTCGGCT GGGGCATCTC TATGTTCCTG 
GTGGGCATCG CTCTGGATCA CTCCACCTCG TTCTCGAATC ATCCCTGTGG TGCCGGCAAC 
AAGGAGAAGA ACTACAACAT CTGCTTCTCC ATCTTTTCGG TGCTGATGAC CTGTGCGATA 
ATCTCCGCAT CGAAGATCAC CTTCAAGTAC GATCCCATCG ATGAACAGTT GGCGGCACAG 
CAGCAGGCGC AGTTCGTGGA TCCCAATAAG CGGGCGGAGG AGGAGTCGAT GAACCAGTTG 
GCGGCCCAGC TAAATCTGCC ATCGCTGGCA GTTGGTAGCG GCAGTGCCGC CTCGGGTGCC 
TGTGCCGGTG GCGTCAGTAG CTTCCTTGCC ACTGGCCATC AGCCACAGCC GCATATTGGT 
GCCGAGTCCA AGGTGTTCGC CCAGACGGCC AAGGAGTTGC CAGAGTGGAT GACCGTGTTG 
ACCCACTTTA AAGATCTGAA AACCGCATCC TTCCTTTTCG TGGCCTGGTT CATGGGCTTC 
GGCATTGGCT TGATCTTCAC CTTTCTGTTC TGGCATCTGC AGGACTATGG TGGCACTCCC 
ACCCTGTTCG GTGTGGCCTC TGTCATCAAT CATGTTTCCG AGATTTTTGC CTACTTCTTT 
AGCTTCCGTC TGATTACCCA AATTGGCCAT GTCAAGGTTT TGTGTCTGGG CTTGATTGGC 
AATGTTCTGC GCTTCCTATA CATTTCCTAT CTGACCAATC CGTGGATGGT GCTGCCATTC 
GAGCTGATGC AGGGCATCAC CCATGCAGCC GTATGGGCTG CATCCTGCTC TTACATTGCC 
CACAACACCC CCAAGCATCT GAGAGCCTCG GCTCAGGGTG TCCTCCAGGG CATCCATCAC 
GGATTGGGTC GTGGCTGCGG CGCCATCATT GGCGGCATGT TTGTCACCTA CTACGGTACT 
ACTACTACCT TCCGCTGGTA CGGCATCGCC TGCCTCTTCG TCCTTGGATT CTTCATCTTC 
GTCAACTTCT ACCGCAAGGA GCAAGGCTTC ATCTCGGAAA TACCCGTCAC CGAGGATCCG 
CATCAGGTGG CCGAGGAGAC CTCGCATTTG GCACCCCACG GCGTTCCCAG CAATCCGATT 
CCACGTGCTC TGTCCAACTC GCGGCTAAAC GAGATGAATC CGAATGGAGG ACCATATGGC 
ACGTACCAGA CCACTGGCGG TAATCTGGAT ATACCCGGTG CCAACCCGCA CAATCCTTTC 
GCTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246323.1
CDS	313..2601
Translation	MNPYTAGASGGVGGGGGANPFGAPAGGAGYGQQGYGYEQQATGGYDQGMNYGIEPQQQAAGYGPPGARPRVDVEATGEVDPNLYPEAKDSTHKVRGHSDILEMFFGASTERELIPVKSFYFFFYAAFGSLFPLMGVYFKQMGMNPGQCGILVGMRPFVEFLSAPFWGSYADRCRQGKRLLLGSLACWVLFTIPLSFIRPEAINCIERRNATDFVLTYTRTKRDLSAMYEQEHLDMGTGTMPADEALEEAEAAHSRHKRSLLLPRIDAGISPVHINFVSNYDDKYHRDYVTPIFSSMVYRTPDIQKAFFLLLLVILIGEFFSAPAITLADSAVITLLGEDADKYGHQRMFGSLGWGISMFLVGIALDHSTSFSNHPCGAGNKEKNYNICFSIFSVLMTCAIISASKITFKYDPIDEQLAAQQQAQFVDPNKRAEEESMNQLAAQLNLPSLAVGSGSAASGACAGGVSSFLATGHQPQPHIGAESKVFAQTAKELPEWMTVLTHFKDLKTASFLFVAWFMGFGIGLIFTFLFWHLQDYGGTPTLFGVASVINHVSEIFAYFFSFRLITQIGHVKVLCLGLIGNVLRFLYISYLTNPWMVLPFELMQGITHAAVWAASCSYIAHNTPKHLRASAQGVLQGIHHGLGRGCGAIIGGMFVTYYGTTTTFRWYGIACLFVLGFFIFVNFYRKEQGFISEIPVTEDPHQVAEETSHLAPHGVPSNPIPRALSNSRLNEMNPNGGPYGTYQTTGGNLDIPGANPHNPFAQ

Target ORF information:

RefSeq Version	NM_001259394.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster jet fuel (jef), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259394.2

ORF Insert Sequence:

ATGAATCCCT ACACTGCAGG CGCCAGCGGA GGTGTCGGTG GCGGAGGAGG AGCCAATCCC 
TTCGGAGCAC CAGCCGGAGG TGCGGGATAT GGCCAGCAGG GCTATGGATA CGAGCAGCAG 
GCAACAGGTG GCTACGACCA GGGCATGAAC TACGGCATTG AGCCGCAGCA GCAGGCAGCC 
GGATACGGAC CACCTGGAGC CAGGCCACGG GTGGATGTGG AGGCCACTGG CGAGGTGGAT 
CCCAATCTAT ATCCAGAGGC GAAGGACTCA ACGCACAAAG TTCGCGGCCA CTCGGACATC 
CTGGAAATGT TTTTCGGGGC GAGCACAGAA CGGGAACTGA TACCCGTGAA AAGCTTCTAC 
TTTTTCTTCT ACGCTGCATT TGGATCACTG TTTCCGCTGA TGGGCGTGTA TTTCAAGCAG 
ATGGGCATGA ATCCGGGCCA GTGCGGAATT CTGGTGGGAA TGCGGCCATT CGTGGAGTTC 
CTGTCGGCCC CGTTTTGGGG CTCCTATGCG GATCGTTGTC GGCAGGGCAA GCGTCTGCTC 
CTGGGCTCCC TGGCCTGTTG GGTACTCTTT ACCATTCCGC TGAGCTTCAT CCGACCGGAG 
GCCATCAATT GCATTGAGCG ACGCAATGCC ACGGACTTTG TGCTGACCTA CACACGCACG 
AAGCGCGACC TGTCGGCCAT GTACGAGCAG GAGCACTTGG ATATGGGCAC TGGCACCATG 
CCAGCCGATG AGGCGCTGGA GGAGGCGGAG GCAGCACACA GTCGGCACAA GAGATCACTG 
CTGCTGCCAA GGATCGATGC GGGCATCTCA CCAGTGCACA TCAACTTTGT GAGCAACTAC 
GACGACAAGT ACCACAGGGA CTATGTGACG CCCATCTTTA GCTCCATGGT GTACAGGACT 
CCGGACATCC AAAAGGCGTT CTTCCTGCTG CTCCTGGTGA TCCTCATTGG GGAGTTCTTC 
AGCGCTCCGG CGATTACCTT GGCTGATTCC GCCGTAATAA CTTTGCTGGG CGAGGATGCG 
GACAAGTACG GTCACCAGCG AATGTTTGGG TCACTCGGCT GGGGCATCTC TATGTTCCTG 
GTGGGCATCG CTCTGGATCA CTCCACCTCG TTCTCGAATC ATCCCTGTGG TGCCGGCAAC 
AAGGAGAAGA ACTACAACAT CTGCTTCTCC ATCTTTTCGG TGCTGATGAC CTGTGCGATA 
ATCTCCGCAT CGAAGATCAC CTTCAAGTAC GATCCCATCG ATGAACAGTT GGCGGCACAG 
CAGCAGGCGC AGTTCGTGGA TCCCAATAAG CGGGCGGAGG AGGAGTCGAT GAACCAGTTG 
GCGGCCCAGC TAAATCTGCC ATCGCTGGCA GTTGGTAGCG GCAGTGCCGC CTCGGGTGCC 
TGTGCCGGTG GCGTCAGTAG CTTCCTTGCC ACTGGCCATC AGCCACAGCC GCATATTGGT 
GCCGAGTCCA AGGTGTTCGC CCAGACGGCC AAGGAGTTGC CAGAGTGGAT GACCGTGTTG 
ACCCACTTTA AAGATCTGAA AACCGCATCC TTCCTTTTCG TGGCCTGGTT CATGGGCTTC 
GGCATTGGCT TGATCTTCAC CTTTCTGTTC TGGCATCTGC AGGACTATGG TGGCACTCCC 
ACCCTGTTCG GTGTGGCCTC TGTCATCAAT CATGTTTCCG AGATTTTTGC CTACTTCTTT 
AGCTTCCGTC TGATTACCCA AATTGGCCAT GTCAAGGTTT TGTGTCTGGG CTTGATTGGC 
AATGTTCTGC GCTTCCTATA CATTTCCTAT CTGACCAATC CGTGGATGGT GCTGCCATTC 
GAGCTGATGC AGGGCATCAC CCATGCAGCC GTATGGGCTG CATCCTGCTC TTACATTGCC 
CACAACACCC CCAAGCATCT GAGAGCCTCG GCTCAGGGTG TCCTCCAGGG CATCCATCAC 
GGATTGGGTC GTGGCTGCGG CGCCATCATT GGCGGCATGT TTGTCACCTA CTACGGTACT 
ACTACTACCT TCCGCTGGTA CGGCATCGCC TGCCTCTTCG TCCTTGGATT CTTCATCTTC 
GTCAACTTCT ACCGCAAGGA GCAAGGCTTC ATCTCGGAAA TACCCGTCAC CGAGGATCCG 
CATCAGGTGG CCGAGGAGAC CTCGCATTTG GCACCCCACG GCGTTCCCAG CAATCCGATT 
CCACGTGCTC TGTCCAACTC GCGGCTAAAC GAGATGAATC CGAATGGAGG ACCATATGGC 
ACGTACCAGA CCACTGGCGG TAATCTGGAT ATACCCGGTG CCAACCCGCA CAATCCTTTC 
GCTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

jef ( NM_137139.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610983.1 Drosophila melanogaster jet fuel (jef), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa10007
Clone ID Related Accession (Same CDS sequence)	NM_001259394.2 , NM_137139.2
Accession Version	NM_137139.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	jet fuel, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137139.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATCCCT ACACTGCAGG CGCCAGCGGA GGTGTCGGTG GCGGAGGAGG AGCCAATCCC 
TTCGGAGCAC CAGCCGGAGG TGCGGGATAT GGCCAGCAGG GCTATGGATA CGAGCAGCAG 
GCAACAGGTG GCTACGACCA GGGCATGAAC TACGGCATTG AGCCGCAGCA GCAGGCAGCC 
GGATACGGAC CACCTGGAGC CAGGCCACGG GTGGATGTGG AGGCCACTGG CGAGGTGGAT 
CCCAATCTAT ATCCAGAGGC GAAGGACTCA ACGCACAAAG TTCGCGGCCA CTCGGACATC 
CTGGAAATGT TTTTCGGGGC GAGCACAGAA CGGGAACTGA TACCCGTGAA AAGCTTCTAC 
TTTTTCTTCT ACGCTGCATT TGGATCACTG TTTCCGCTGA TGGGCGTGTA TTTCAAGCAG 
ATGGGCATGA ATCCGGGCCA GTGCGGAATT CTGGTGGGAA TGCGGCCATT CGTGGAGTTC 
CTGTCGGCCC CGTTTTGGGG CTCCTATGCG GATCGTTGTC GGCAGGGCAA GCGTCTGCTC 
CTGGGCTCCC TGGCCTGTTG GGTACTCTTT ACCATTCCGC TGAGCTTCAT CCGACCGGAG 
GCCATCAATT GCATTGAGCG ACGCAATGCC ACGGACTTTG TGCTGACCTA CACACGCACG 
AAGCGCGACC TGTCGGCCAT GTACGAGCAG GAGCACTTGG ATATGGGCAC TGGCACCATG 
CCAGCCGATG AGGCGCTGGA GGAGGCGGAG GCAGCACACA GTCGGCACAA GAGATCACTG 
CTGCTGCCAA GGATCGATGC GGGCATCTCA CCAGTGCACA TCAACTTTGT GAGCAACTAC 
GACGACAAGT ACCACAGGGA CTATGTGACG CCCATCTTTA GCTCCATGGT GTACAGGACT 
CCGGACATCC AAAAGGCGTT CTTCCTGCTG CTCCTGGTGA TCCTCATTGG GGAGTTCTTC 
AGCGCTCCGG CGATTACCTT GGCTGATTCC GCCGTAATAA CTTTGCTGGG CGAGGATGCG 
GACAAGTACG GTCACCAGCG AATGTTTGGG TCACTCGGCT GGGGCATCTC TATGTTCCTG 
GTGGGCATCG CTCTGGATCA CTCCACCTCG TTCTCGAATC ATCCCTGTGG TGCCGGCAAC 
AAGGAGAAGA ACTACAACAT CTGCTTCTCC ATCTTTTCGG TGCTGATGAC CTGTGCGATA 
ATCTCCGCAT CGAAGATCAC CTTCAAGTAC GATCCCATCG ATGAACAGTT GGCGGCACAG 
CAGCAGGCGC AGTTCGTGGA TCCCAATAAG CGGGCGGAGG AGGAGTCGAT GAACCAGTTG 
GCGGCCCAGC TAAATCTGCC ATCGCTGGCA GTTGGTAGCG GCAGTGCCGC CTCGGGTGCC 
TGTGCCGGTG GCGTCAGTAG CTTCCTTGCC ACTGGCCATC AGCCACAGCC GCATATTGGT 
GCCGAGTCCA AGGTGTTCGC CCAGACGGCC AAGGAGTTGC CAGAGTGGAT GACCGTGTTG 
ACCCACTTTA AAGATCTGAA AACCGCATCC TTCCTTTTCG TGGCCTGGTT CATGGGCTTC 
GGCATTGGCT TGATCTTCAC CTTTCTGTTC TGGCATCTGC AGGACTATGG TGGCACTCCC 
ACCCTGTTCG GTGTGGCCTC TGTCATCAAT CATGTTTCCG AGATTTTTGC CTACTTCTTT 
AGCTTCCGTC TGATTACCCA AATTGGCCAT GTCAAGGTTT TGTGTCTGGG CTTGATTGGC 
AATGTTCTGC GCTTCCTATA CATTTCCTAT CTGACCAATC CGTGGATGGT GCTGCCATTC 
GAGCTGATGC AGGGCATCAC CCATGCAGCC GTATGGGCTG CATCCTGCTC TTACATTGCC 
CACAACACCC CCAAGCATCT GAGAGCCTCG GCTCAGGGTG TCCTCCAGGG CATCCATCAC 
GGATTGGGTC GTGGCTGCGG CGCCATCATT GGCGGCATGT TTGTCACCTA CTACGGTACT 
ACTACTACCT TCCGCTGGTA CGGCATCGCC TGCCTCTTCG TCCTTGGATT CTTCATCTTC 
GTCAACTTCT ACCGCAAGGA GCAAGGCTTC ATCTCGGAAA TACCCGTCAC CGAGGATCCG 
CATCAGGTGG CCGAGGAGAC CTCGCATTTG GCACCCCACG GCGTTCCCAG CAATCCGATT 
CCACGTGCTC TGTCCAACTC GCGGCTAAAC GAGATGAATC CGAATGGAGG ACCATATGGC 
ACGTACCAGA CCACTGGCGG TAATCTGGAT ATACCCGGTG CCAACCCGCA CAATCCTTTC 
GCTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610983.1
CDS	313..2601
Translation	MNPYTAGASGGVGGGGGANPFGAPAGGAGYGQQGYGYEQQATGGYDQGMNYGIEPQQQAAGYGPPGARPRVDVEATGEVDPNLYPEAKDSTHKVRGHSDILEMFFGASTERELIPVKSFYFFFYAAFGSLFPLMGVYFKQMGMNPGQCGILVGMRPFVEFLSAPFWGSYADRCRQGKRLLLGSLACWVLFTIPLSFIRPEAINCIERRNATDFVLTYTRTKRDLSAMYEQEHLDMGTGTMPADEALEEAEAAHSRHKRSLLLPRIDAGISPVHINFVSNYDDKYHRDYVTPIFSSMVYRTPDIQKAFFLLLLVILIGEFFSAPAITLADSAVITLLGEDADKYGHQRMFGSLGWGISMFLVGIALDHSTSFSNHPCGAGNKEKNYNICFSIFSVLMTCAIISASKITFKYDPIDEQLAAQQQAQFVDPNKRAEEESMNQLAAQLNLPSLAVGSGSAASGACAGGVSSFLATGHQPQPHIGAESKVFAQTAKELPEWMTVLTHFKDLKTASFLFVAWFMGFGIGLIFTFLFWHLQDYGGTPTLFGVASVINHVSEIFAYFFSFRLITQIGHVKVLCLGLIGNVLRFLYISYLTNPWMVLPFELMQGITHAAVWAASCSYIAHNTPKHLRASAQGVLQGIHHGLGRGCGAIIGGMFVTYYGTTTTFRWYGIACLFVLGFFIFVNFYRKEQGFISEIPVTEDPHQVAEETSHLAPHGVPSNPIPRALSNSRLNEMNPNGGPYGTYQTTGGNLDIPGANPHNPFAQ

Target ORF information:

RefSeq Version	NM_137139.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster jet fuel (jef), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137139.2

ORF Insert Sequence:

ATGAATCCCT ACACTGCAGG CGCCAGCGGA GGTGTCGGTG GCGGAGGAGG AGCCAATCCC 
TTCGGAGCAC CAGCCGGAGG TGCGGGATAT GGCCAGCAGG GCTATGGATA CGAGCAGCAG 
GCAACAGGTG GCTACGACCA GGGCATGAAC TACGGCATTG AGCCGCAGCA GCAGGCAGCC 
GGATACGGAC CACCTGGAGC CAGGCCACGG GTGGATGTGG AGGCCACTGG CGAGGTGGAT 
CCCAATCTAT ATCCAGAGGC GAAGGACTCA ACGCACAAAG TTCGCGGCCA CTCGGACATC 
CTGGAAATGT TTTTCGGGGC GAGCACAGAA CGGGAACTGA TACCCGTGAA AAGCTTCTAC 
TTTTTCTTCT ACGCTGCATT TGGATCACTG TTTCCGCTGA TGGGCGTGTA TTTCAAGCAG 
ATGGGCATGA ATCCGGGCCA GTGCGGAATT CTGGTGGGAA TGCGGCCATT CGTGGAGTTC 
CTGTCGGCCC CGTTTTGGGG CTCCTATGCG GATCGTTGTC GGCAGGGCAA GCGTCTGCTC 
CTGGGCTCCC TGGCCTGTTG GGTACTCTTT ACCATTCCGC TGAGCTTCAT CCGACCGGAG 
GCCATCAATT GCATTGAGCG ACGCAATGCC ACGGACTTTG TGCTGACCTA CACACGCACG 
AAGCGCGACC TGTCGGCCAT GTACGAGCAG GAGCACTTGG ATATGGGCAC TGGCACCATG 
CCAGCCGATG AGGCGCTGGA GGAGGCGGAG GCAGCACACA GTCGGCACAA GAGATCACTG 
CTGCTGCCAA GGATCGATGC GGGCATCTCA CCAGTGCACA TCAACTTTGT GAGCAACTAC 
GACGACAAGT ACCACAGGGA CTATGTGACG CCCATCTTTA GCTCCATGGT GTACAGGACT 
CCGGACATCC AAAAGGCGTT CTTCCTGCTG CTCCTGGTGA TCCTCATTGG GGAGTTCTTC 
AGCGCTCCGG CGATTACCTT GGCTGATTCC GCCGTAATAA CTTTGCTGGG CGAGGATGCG 
GACAAGTACG GTCACCAGCG AATGTTTGGG TCACTCGGCT GGGGCATCTC TATGTTCCTG 
GTGGGCATCG CTCTGGATCA CTCCACCTCG TTCTCGAATC ATCCCTGTGG TGCCGGCAAC 
AAGGAGAAGA ACTACAACAT CTGCTTCTCC ATCTTTTCGG TGCTGATGAC CTGTGCGATA 
ATCTCCGCAT CGAAGATCAC CTTCAAGTAC GATCCCATCG ATGAACAGTT GGCGGCACAG 
CAGCAGGCGC AGTTCGTGGA TCCCAATAAG CGGGCGGAGG AGGAGTCGAT GAACCAGTTG 
GCGGCCCAGC TAAATCTGCC ATCGCTGGCA GTTGGTAGCG GCAGTGCCGC CTCGGGTGCC 
TGTGCCGGTG GCGTCAGTAG CTTCCTTGCC ACTGGCCATC AGCCACAGCC GCATATTGGT 
GCCGAGTCCA AGGTGTTCGC CCAGACGGCC AAGGAGTTGC CAGAGTGGAT GACCGTGTTG 
ACCCACTTTA AAGATCTGAA AACCGCATCC TTCCTTTTCG TGGCCTGGTT CATGGGCTTC 
GGCATTGGCT TGATCTTCAC CTTTCTGTTC TGGCATCTGC AGGACTATGG TGGCACTCCC 
ACCCTGTTCG GTGTGGCCTC TGTCATCAAT CATGTTTCCG AGATTTTTGC CTACTTCTTT 
AGCTTCCGTC TGATTACCCA AATTGGCCAT GTCAAGGTTT TGTGTCTGGG CTTGATTGGC 
AATGTTCTGC GCTTCCTATA CATTTCCTAT CTGACCAATC CGTGGATGGT GCTGCCATTC 
GAGCTGATGC AGGGCATCAC CCATGCAGCC GTATGGGCTG CATCCTGCTC TTACATTGCC 
CACAACACCC CCAAGCATCT GAGAGCCTCG GCTCAGGGTG TCCTCCAGGG CATCCATCAC 
GGATTGGGTC GTGGCTGCGG CGCCATCATT GGCGGCATGT TTGTCACCTA CTACGGTACT 
ACTACTACCT TCCGCTGGTA CGGCATCGCC TGCCTCTTCG TCCTTGGATT CTTCATCTTC 
GTCAACTTCT ACCGCAAGGA GCAAGGCTTC ATCTCGGAAA TACCCGTCAC CGAGGATCCG 
CATCAGGTGG CCGAGGAGAC CTCGCATTTG GCACCCCACG GCGTTCCCAG CAATCCGATT 
CCACGTGCTC TGTCCAACTC GCGGCTAAAC GAGATGAATC CGAATGGAGG ACCATATGGC 
ACGTACCAGA CCACTGGCGG TAATCTGGAT ATACCCGGTG CCAACCCGCA CAATCCTTTC 
GCTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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jef cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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