Mfsd6 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mfsd6
Entrez Gene ID	98682
Full Name	major facilitator superfamily domain containing 6
Synonyms	2210010L05Rik,9630025I22Rik,AI836200,AW212394,MMR2
General protein information	Preferred Names major facilitator superfamily domain containing 6 Names major facilitator superfamily domain-containing protein 6 macrophage MHC class I receptor 2 macrophage MHC receptor 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 C1.1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006496374.3	XP_006496437.1	major facilitator superfamily domain-containing protein 6 isoform X1
NM_133829.2	NP_598590.2	major facilitator superfamily domain-containing protein 6 isoform 1
XM_006496375.2	XP_006496438.1	major facilitator superfamily domain-containing protein 6 isoform X2
NM_001357464.1	NP_001344393.1	major facilitator superfamily domain-containing protein 6 isoform 3
NM_178081.4	NP_835182.1	major facilitator superfamily domain-containing protein 6 isoform 2
XM_006496374.4	XP_006496437.1	major facilitator superfamily domain-containing protein 6 isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	mfsd6a	NP_001076525.1
Homo sapiens (human)	MFSD6	NP_060164.3
Bos taurus (cattle)	MFSD6	XP_005202303.1
Gallus gallus (chicken)	MFSD6	XP_421837.1
	MFSD6	XP_001108155.1
Canis lupus familiaris (dog)	MFSD6	XP_005640516.1
Drosophila melanogaster (fruit fly)	CG12858	NP_001246323.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003204	XP_312908.4
Xenopus tropicalis (tropical clawed frog)	mfsd6	XP_002941640.1
Pan troglodytes (chimpanzee)	MFSD6	XP_001166645.1
Rattus norvegicus (Norway rat)	Mfsd6	NP_001100381.1
Caenorhabditis elegans (roundworm)	R13A5.9	NP_498662.1
Mus musculus (house mouse)	Mfsd6	NP_598590.2

Gene Ontology
Bibliography

Related articles in PubMed

Macrophage MHC and T-cell receptors essential for rejection of allografted skin and lymphoma.
Tashiro-Yamaji J, Maeda S, Ikawa M, Okabe M, Kubota T, Yoshida R
Transplantation96(3)251-7(2013 Aug)

A mutation in the Srrm4 gene causes alternative splicing defects and deafness in the Bronx waltzer mouse.
Nakano Y, Jahan I, Bonde G, Sun X, Hildebrand MS, Engelhardt JF, Smith RJ, Cornell RA, Fritzsch B, B?nfi B
PLoS genetics8(10)e1002966(2012)

HLA-B62 as a possible ligand for the human homologue of mouse macrophage MHC receptor 2 (MMR2) on monocytes.
Shimizu T, Tashiro-Yamaji J, Hayashi M, Inoue Y, Ibata M, Kubota T, Tanigawa N, Yoshida R
Gene454(1-2)31-8(2010 Apr)

Macrophage MHC receptor 2: a novel receptor on allograft (H-2D(d)K(d))-induced macrophage (H-2D(b)K(b)) recognizing an MHC class I molecule, H-2K(d), in mice.
Tashiro-Yamaji J, Kubota T, Yoshida R
Gene3841-8(2006 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

MMRs on monocytes/macrophages and TCRs on cytotoxic T lymphocytes in mice were essential for recognition and rejection of allografted skin and lymphoma, respectively.
Title: Macrophage MHC and T-cell receptors essential for rejection of allografted skin and lymphoma.

isolation of a cDNA clone encoding a novel receptor (MMR2) on allograft (H-2D(d)K(d))-induced macrophages (AIM) recognizing another MHC class I molecule, H-2K(d), by the expression cloning method
Title: Macrophage MHC receptor 2: a novel receptor on allograft (H-2D(d)K(d))-induced macrophage (H-2D(b)K(b)) recognizing an MHC class I molecule, H-2K(d), in mice.

The following Mfsd6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mfsd6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu07085	NM_178081.4 Latest version!	Mus musculus major facilitator superfamily domain containing 6 (Mfsd6), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$454.30 $649.00
OMu10260	NM_133829.2 Latest version!	Mus musculus major facilitator superfamily domain containing 6 (Mfsd6), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu27685	NM_001357464.1 Latest version!	Mus musculus major facilitator superfamily domain containing 6 (Mfsd6), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu27685	XM_006496375.2 Latest version!	Mus musculus major facilitator superfamily domain containing 6 (Mfsd6), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu10260	XM_006496374.4 Latest version!	Mus musculus major facilitator superfamily domain containing 6 (Mfsd6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu10260	XM_006496374.3	Mus musculus major facilitator superfamily domain containing 6 (Mfsd6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mfsd6 ( NM_178081.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_835182.1 Mus musculus major facilitator superfamily domain containing 6 (Mfsd6), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu07085
Clone ID Related Accession (Same CDS sequence)	NM_178081.4
Accession Version	NM_178081.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Mus musculus(house mouse)
Product	major facilitator superfamily domain-containing protein 6 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC139299.3 and AC161876.3. On May 2, 2007 this sequence version replaced NM_178081.3. Transcript Variant: This variant (2) lacks an exon in the coding region, which results in an early stop codon, compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK035995.1, AK046866.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGCTG ATGATAAAGT TGCTATCCTA ACTGATGATG AAGAGGAGCA GAAGAGAAAA 
TATGTGCTTG CTGATCCCTT CAATGGCATC TGCAGGGAAC CAGAACCACC TTCTAATGAA 
ACCCCCTCCT CCACAGAGAC CTCAGCCATT CCCGAGGAGG AAATAGACTG GATAGAGAAA 
CATTGTGTGA AAGTCAACAA TGACCTTCTC ATCTCCAAGG TGTTTTATTT TTTCTTTTAC 
TCTGCCTATG GCTCTCTTTA CCCTCTCTTA CCTGTATATT ATAAACAGCT GGGAATGTCA 
CCTAGCCAGA GTGGACTGTT GGTGGGCATT CGATACTTCA TTGAATTCTG CAGTGCCCCC 
TTCTGGGGTG TAGTTGCAGA TCGTTTCAGA AAGGGCAAAA TTGTCCTCCT CTTTTCGCTT 
CTGTGTTGGG TTTTGTTCAA CCTGGGCATT GGATTTGTCA AACCTGCTAC CTTGAGATGT 
CTACCAAAGA TCCCCCCAAC AGCTCACCCC ACCAATGTAA GTCACCCAGT AACTGTTCTG 
CCAATGAACT CCTCCACTGT CGCTTTCTTT AGCACACCAC CAAAATTGCT CCAGAAAAGG 
GACGTGCAAT TGTCTGAAAC AGAGCCCAAT ATCTCAGACA TAGATCTCGT GTCAACAGCA 
TTGACCTTGA CCAGTGAACC CACACGGAGG CCCCAGACAG AGGCAATTAC CCACCCTGTT 
ACAGGCTTGA TTTTGAACAC AAGCACAGTG ACTCTGCCCC CCACAGGAAA TGTCACCAGG 
GAGACAACCA TTGCTGTGGT CACCACCACC AAATCTTTAC CATCTGATCA AGTCACCCTT 
GTGTATGACC AACAGGAAGT TGAGGCGATA TTCTTAATTA TCTTAGTGGT TGTCATAATA 
GGAGAATTTT TCAGTGCCTC TTCTGTCACG ATTGTAGATA CAGTAACCCT CCAGTACCTG 
GGAAAACACC GAGACCGATA TGGGCTACAG CGCATGTGGG GCTCCCTGGG CTGGGGCCTG 
GCCATGCTGT CTGTGGGTAT TGGAATCGAC TATACTCATA TAGATGTGCT CATTGATGGG 
AAGGGGTGTA AGCCCCCTGA GTACCGGAAC TACCAGATTG TCTTCATCGT CTTTGGAGTT 
CTCATGACCA TGGCCCTGAT CGTTGCCACT CAGTTCCGGT TCCGCTACAA CCACTTCAAC 
AATAGTGATG GTAAAGGGAA AGAGGTGGAG ATCCCACAGG TGGAAAGGGA CAACTCCACA 
GAGTCCTCTG AAGAGACTCC AACTGCCGCG ACTCACTCAC AGGCCTTCAA CTTCTGGGAC 
TTGATCAAGT TGCTATGCAG TGTGCAGTAT GGCTCGGTTC TGTTTGTGGC CTGGTTCATG 
GGTTTTGGAT ACGGCTTCGT GTTCACCTTT CTCTACTGGC ATCTGGAAGA CCTAAATGGA 
ACTACAACCC TCTTTGGGGT CTGTTCAGTC CTGAGTCATG TGTCCGAGTT GACCGCTTAC 
TTCTTCAGCC ACAAGCTTAT TGAGTTGATT GGCCATATCA GGGTTTTATA CATTGGCCTG 
GCCTGTAATA CAGCTCGCTA TATTTACATT TCCTACCTGG AAAATGCCTG GACTGTCCTC 
CCTATGGAAG TTCTTCAAGG TGTCACACAC GCAGCCATCT GGGCAGCGTG CATCTCTTAC 
CTCAGTGCAG CTGTCCCCCC TGAGCTGAGG ACTTCTGCAC AGGGAATCCT GCAAGGCCTT 
CATCTGGGTC TGGGGCGAGG ATGTGGTGCC ATGATCGGAG GCGTGTTAGT CAATTATTTT 
GGGGCTGCTG CAACTTTCCG AGGGATTGGC ATGGCTTGCC TGGTGATCCT TCTCCTCTTT 
GCCCTGATCC AGTGGCTGGC AGTACCAGAT GAAGAAGAAG ACAAGACAAT GTTGGCAGAA 
AGGATCCCAG TTCCCTCTAG TCCCGTTCCC ATAGCAACCA TTGACCTGGT ACAGCAACAG 
ACAGAAGATG TCATGCCACG CGTTGAGGCC AGACTTCCGC CCAAGAAAAC CAAGCACCAG 
GAAGAGCAGG AAGATGTAAA TAAACCGGCC TGGGGGGTCA GCTCCTCGCC TTGGGTGACC 
TTTGTCTATG CTCTCTACCA AGTTAAAGAG CTAATTCAAC TGACAAGAGA GAGCCGTGCT 
TCTGAGATAC AACCTTTACA GGTAACCCTC TGCTGGGCTA GCGTGGCGTC TGCACCGTTA 
CTCCCTCCTT GTTCATCAAA ACACATGGGA AACAGAAAAA CAGGCATGTT AGCAAAGGAT 
ATCAGTGGTC TGCGATCACT CTGTCACAGT GTTTACCAGG TAGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_835182.1
CDS	348..2675
Translation	MAADDKVAILTDDEEEQKRKYVLADPFNGICREPEPPSNETPSSTETSAIPEEEIDWIEKHCVKVNNDLLISKVFYFFFYSAYGSLYPLLPVYYKQLGMSPSQSGLLVGIRYFIEFCSAPFWGVVADRFRKGKIVLLFSLLCWVLFNLGIGFVKPATLRCLPKIPPTAHPTNVSHPVTVLPMNSSTVAFFSTPPKLLQKRDVQLSETEPNISDIDLVSTALTLTSEPTRRPQTEAITHPVTGLILNTSTVTLPPTGNVTRETTIAVVTTTKSLPSDQVTLVYDQQEVEAIFLIILVVVIIGEFFSASSVTIVDTVTLQYLGKHRDRYGLQRMWGSLGWGLAMLSVGIGIDYTHIDVLIDGKGCKPPEYRNYQIVFIVFGVLMTMALIVATQFRFRYNHFNNSDGKGKEVEIPQVERDNSTESSEETPTAATHSQAFNFWDLIKLLCSVQYGSVLFVAWFMGFGYGFVFTFLYWHLEDLNGTTTLFGVCSVLSHVSELTAYFFSHKLIELIGHIRVLYIGLACNTARYIYISYLENAWTVLPMEVLQGVTHAAIWAACISYLSAAVPPELRTSAQGILQGLHLGLGRGCGAMIGGVLVNYFGAAATFRGIGMACLVILLLFALIQWLAVPDEEEDKTMLAERIPVPSSPVPIATIDLVQQQTEDVMPRVEARLPPKKTKHQEEQEDVNKPAWGVSSSPWVTFVYALYQVKELIQLTRESRASEIQPLQVTLCWASVASAPLLPPCSSKHMGNRKTGMLAKDISGLRSLCHSVYQVA

Target ORF information:

RefSeq Version	NM_178081.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus major facilitator superfamily domain containing 6 (Mfsd6), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178081.4

ORF Insert Sequence:

ATGGCTGCTG ATGATAAAGT TGCTATCCTA ACTGATGATG AAGAGGAGCA GAAGAGAAAA 
TATGTGCTTG CTGATCCCTT CAATGGCATC TGCAGGGAAC CAGAACCACC TTCTAATGAA 
ACCCCCTCCT CCACAGAGAC CTCAGCCATT CCCGAGGAGG AAATAGACTG GATAGAGAAA 
CATTGTGTGA AAGTCAACAA TGACCTTCTC ATCTCCAAGG TGTTTTATTT TTTCTTTTAC 
TCTGCCTATG GCTCTCTTTA CCCTCTCTTA CCTGTATATT ATAAACAGCT GGGAATGTCA 
CCTAGCCAGA GTGGACTGTT GGTGGGCATT CGATACTTCA TTGAATTCTG CAGTGCCCCC 
TTCTGGGGTG TAGTTGCAGA TCGTTTCAGA AAGGGCAAAA TTGTCCTCCT CTTTTCGCTT 
CTGTGTTGGG TTTTGTTCAA CCTGGGCATT GGATTTGTCA AACCTGCTAC CTTGAGATGT 
CTACCAAAGA TCCCCCCAAC AGCTCACCCC ACCAATGTAA GTCACCCAGT AACTGTTCTG 
CCAATGAACT CCTCCACTGT CGCTTTCTTT AGCACACCAC CAAAATTGCT CCAGAAAAGG 
GACGTGCAAT TGTCTGAAAC AGAGCCCAAT ATCTCAGACA TAGATCTCGT GTCAACAGCA 
TTGACCTTGA CCAGTGAACC CACACGGAGG CCCCAGACAG AGGCAATTAC CCACCCTGTT 
ACAGGCTTGA TTTTGAACAC AAGCACAGTG ACTCTGCCCC CCACAGGAAA TGTCACCAGG 
GAGACAACCA TTGCTGTGGT CACCACCACC AAATCTTTAC CATCTGATCA AGTCACCCTT 
GTGTATGACC AACAGGAAGT TGAGGCGATA TTCTTAATTA TCTTAGTGGT TGTCATAATA 
GGAGAATTTT TCAGTGCCTC TTCTGTCACG ATTGTAGATA CAGTAACCCT CCAGTACCTG 
GGAAAACACC GAGACCGATA TGGGCTACAG CGCATGTGGG GCTCCCTGGG CTGGGGCCTG 
GCCATGCTGT CTGTGGGTAT TGGAATCGAC TATACTCATA TAGATGTGCT CATTGATGGG 
AAGGGGTGTA AGCCCCCTGA GTACCGGAAC TACCAGATTG TCTTCATCGT CTTTGGAGTT 
CTCATGACCA TGGCCCTGAT CGTTGCCACT CAGTTCCGGT TCCGCTACAA CCACTTCAAC 
AATAGTGATG GTAAAGGGAA AGAGGTGGAG ATCCCACAGG TGGAAAGGGA CAACTCCACA 
GAGTCCTCTG AAGAGACTCC AACTGCCGCG ACTCACTCAC AGGCCTTCAA CTTCTGGGAC 
TTGATCAAGT TGCTATGCAG TGTGCAGTAT GGCTCGGTTC TGTTTGTGGC CTGGTTCATG 
GGTTTTGGAT ACGGCTTCGT GTTCACCTTT CTCTACTGGC ATCTGGAAGA CCTAAATGGA 
ACTACAACCC TCTTTGGGGT CTGTTCAGTC CTGAGTCATG TGTCCGAGTT GACCGCTTAC 
TTCTTCAGCC ACAAGCTTAT TGAGTTGATT GGCCATATCA GGGTTTTATA CATTGGCCTG 
GCCTGTAATA CAGCTCGCTA TATTTACATT TCCTACCTGG AAAATGCCTG GACTGTCCTC 
CCTATGGAAG TTCTTCAAGG TGTCACACAC GCAGCCATCT GGGCAGCGTG CATCTCTTAC 
CTCAGTGCAG CTGTCCCCCC TGAGCTGAGG ACTTCTGCAC AGGGAATCCT GCAAGGCCTT 
CATCTGGGTC TGGGGCGAGG ATGTGGTGCC ATGATCGGAG GCGTGTTAGT CAATTATTTT 
GGGGCTGCTG CAACTTTCCG AGGGATTGGC ATGGCTTGCC TGGTGATCCT TCTCCTCTTT 
GCCCTGATCC AGTGGCTGGC AGTACCAGAT GAAGAAGAAG ACAAGACAAT GTTGGCAGAA 
AGGATCCCAG TTCCCTCTAG TCCCGTTCCC ATAGCAACCA TTGACCTGGT ACAGCAACAG 
ACAGAAGATG TCATGCCACG CGTTGAGGCC AGACTTCCGC CCAAGAAAAC CAAGCACCAG 
GAAGAGCAGG AAGATGTAAA TAAACCGGCC TGGGGGGTCA GCTCCTCGCC TTGGGTGACC 
TTTGTCTATG CTCTCTACCA AGTTAAAGAG CTAATTCAAC TGACAAGAGA GAGCCGTGCT 
TCTGAGATAC AACCTTTACA GGTAACCCTC TGCTGGGCTA GCGTGGCGTC TGCACCGTTA 
CTCCCTCCTT GTTCATCAAA ACACATGGGA AACAGAAAAA CAGGCATGTT AGCAAAGGAT 
ATCAGTGGTC TGCGATCACT CTGTCACAGT GTTTACCAGG TAGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mfsd6 ( NM_133829.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_598590.2 Mus musculus major facilitator superfamily domain containing 6 (Mfsd6), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu10260
Clone ID Related Accession (Same CDS sequence)	XM_006496374.3 , NM_133829.2 , XM_006496374.4
Accession Version	NM_133829.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Mus musculus(house mouse)
Product	major facilitator superfamily domain-containing protein 6 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC139299.3 and AC161876.3. On Nov 20, 2007 this sequence version replaced NM_133829.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGCTG ATGATAAAGT TGCTATCCTA ACTGATGATG AAGAGGAGCA GAAGAGAAAA 
TATGTGCTTG CTGATCCCTT CAATGGCATC TGCAGGGAAC CAGAACCACC TTCTAATGAA 
ACCCCCTCCT CCACAGAGAC CTCAGCCATT CCCGAGGAGG AAATAGACTG GATAGAGAAA 
CATTGTGTGA AAGTCAACAA TGACCTTCTC ATCTCCAAGG TGTTTTATTT TTTCTTTTAC 
TCTGCCTATG GCTCTCTTTA CCCTCTCTTA CCTGTATATT ATAAACAGCT GGGAATGTCA 
CCTAGCCAGA GTGGACTGTT GGTGGGCATT CGATACTTCA TTGAATTCTG CAGTGCCCCC 
TTCTGGGGTG TAGTTGCAGA TCGTTTCAGA AAGGGCAAAA TTGTCCTCCT CTTTTCGCTT 
CTGTGTTGGG TTTTGTTCAA CCTGGGCATT GGATTTGTCA AACCTGCTAC CTTGAGATGT 
CTACCAAAGA TCCCCCCAAC AGCTCACCCC ACCAATGTAA GTCACCCAGT AACTGTTCTG 
CCAATGAACT CCTCCACTGT CGCTTTCTTT AGCACACCAC CAAAATTGCT CCAGAAAAGG 
GACGTGCAAT TGTCTGAAAC AGAGCCCAAT ATCTCAGACA TAGATCTCGT GTCAACAGCA 
TTGACCTTGA CCAGTGAACC CACACGGAGG CCCCAGACAG AGGCAATTAC CCACCCTGTT 
ACAGGCTTGA TTTTGAACAC AAGCACAGTG ACTCTGCCCC CCACAGGAAA TGTCACCAGG 
GAGACAACCA TTGCTGTGGT CACCACCACC AAATCTTTAC CATCTGATCA AGTCACCCTT 
GTGTATGACC AACAGGAAGT TGAGGCGATA TTCTTAATTA TCTTAGTGGT TGTCATAATA 
GGAGAATTTT TCAGTGCCTC TTCTGTCACG ATTGTAGATA CAGTAACCCT CCAGTACCTG 
GGAAAACACC GAGACCGATA TGGGCTACAG CGCATGTGGG GCTCCCTGGG CTGGGGCCTG 
GCCATGCTGT CTGTGGGTAT TGGAATCGAC TATACTCATA TAGATGTGCT CATTGATGGG 
AAGGGGTGTA AGCCCCCTGA GTACCGGAAC TACCAGATTG TCTTCATCGT CTTTGGAGTT 
CTCATGACCA TGGCCCTGAT CGTTGCCACT CAGTTCCGGT TCCGCTACAA CCACTTCAAC 
AATAGTGATG GTAAAGGGAA AGAGGTGGAG ATCCCACAGG TGGAAAGGGA CAACTCCACA 
GAGTCCTCTG AAGAGACTCC AACTGCCGCG ACTCACTCAC AGGCCTTCAA CTTCTGGGAC 
TTGATCAAGT TGCTATGCAG TGTGCAGTAT GGCTCGGTTC TGTTTGTGGC CTGGTTCATG 
GGTTTTGGAT ACGGCTTCGT GTTCACCTTT CTCTACTGGC ATCTGGAAGA CCTAAATGGA 
ACTACAACCC TCTTTGGGGT CTGTTCAGTC CTGAGTCATG TGTCCGAGTT GACCGCTTAC 
TTCTTCAGCC ACAAGCTTAT TGAGTTGATT GGCCATATCA GGGTTTTATA CATTGGCCTG 
GCCTGTAATA CAGCTCGCTA TATTTACATT TCCTACCTGG AAAATGCCTG GACTGTCCTC 
CCTATGGAAG TTCTTCAAGG TGTCACACAC GCAGCCATCT GGGCAGCGTG CATCTCTTAC 
CTCAGTGCAG CTGTCCCCCC TGAGCTGAGG ACTTCTGCAC AGGGAATCCT GCAAGGCCTT 
CATCTGGGTC TGGGGCGAGG ATGTGGTGCC ATGATCGGAG GCGTGTTAGT CAATTATTTT 
GGGGCTGCTG CAACTTTCCG AGGGATTGGC ATGGCTTGCC TGGTGATCCT TCTCCTCTTT 
GCCCTGATCC AGTGGCTGGC AGTACCAGAT GAAGAAGAAG ACAAGACAAT GTTGGCAGAA 
AGGATCCCAG TTCCCTCTAG TCCCGTTCCC ATAGCAACCA TTGACCTGGT ACAGCAACAG 
ACAGAAGATG TCATGCCACG CGTTGAGGCC AGACTTCCGC CCAAGAAAAC CAAGCACCAG 
GAAGAGCAGG AAGATGTAAA TAAACCGGCC TGGGGGGTCA GCTCCTCGCC TTGGGTGACC 
TTTGTCTATG CTCTCTACCA AGTTAAAGAG CTAATTCAAC TGACAAGAGA GAGCCGTGCT 
TCTGAGATAC AACCTTTACA GGGGACCAGT GAGAATCGGG AAGCCTCTCC AGCTGGTGGA 
GCCCAGCGTG CCCCACGTGA AACTCACTCC GCCTCACCTA GAAACCAGCC ATCCCCTGAC 
ACAGCAGCAT CTCAAACGCA GTCCAGCCCT GCTCACCCCA GTGCTACCCC GCATGGACAG 
GAGAGTGGAG AGAAGCAGGC TCAGCCCACT GTGGGAGAAC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598590.2
CDS	348..2732
Translation	MAADDKVAILTDDEEEQKRKYVLADPFNGICREPEPPSNETPSSTETSAIPEEEIDWIEKHCVKVNNDLLISKVFYFFFYSAYGSLYPLLPVYYKQLGMSPSQSGLLVGIRYFIEFCSAPFWGVVADRFRKGKIVLLFSLLCWVLFNLGIGFVKPATLRCLPKIPPTAHPTNVSHPVTVLPMNSSTVAFFSTPPKLLQKRDVQLSETEPNISDIDLVSTALTLTSEPTRRPQTEAITHPVTGLILNTSTVTLPPTGNVTRETTIAVVTTTKSLPSDQVTLVYDQQEVEAIFLIILVVVIIGEFFSASSVTIVDTVTLQYLGKHRDRYGLQRMWGSLGWGLAMLSVGIGIDYTHIDVLIDGKGCKPPEYRNYQIVFIVFGVLMTMALIVATQFRFRYNHFNNSDGKGKEVEIPQVERDNSTESSEETPTAATHSQAFNFWDLIKLLCSVQYGSVLFVAWFMGFGYGFVFTFLYWHLEDLNGTTTLFGVCSVLSHVSELTAYFFSHKLIELIGHIRVLYIGLACNTARYIYISYLENAWTVLPMEVLQGVTHAAIWAACISYLSAAVPPELRTSAQGILQGLHLGLGRGCGAMIGGVLVNYFGAAATFRGIGMACLVILLLFALIQWLAVPDEEEDKTMLAERIPVPSSPVPIATIDLVQQQTEDVMPRVEARLPPKKTKHQEEQEDVNKPAWGVSSSPWVTFVYALYQVKELIQLTRESRASEIQPLQGTSENREASPAGGAQRAPRETHSASPRNQPSPDTAASQTQSSPAHPSATPHGQESGEKQAQPTVGEH

Target ORF information:

RefSeq Version	NM_133829.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus major facilitator superfamily domain containing 6 (Mfsd6), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133829.2

ORF Insert Sequence:

ATGGCTGCTG ATGATAAAGT TGCTATCCTA ACTGATGATG AAGAGGAGCA GAAGAGAAAA 
TATGTGCTTG CTGATCCCTT CAATGGCATC TGCAGGGAAC CAGAACCACC TTCTAATGAA 
ACCCCCTCCT CCACAGAGAC CTCAGCCATT CCCGAGGAGG AAATAGACTG GATAGAGAAA 
CATTGTGTGA AAGTCAACAA TGACCTTCTC ATCTCCAAGG TGTTTTATTT TTTCTTTTAC 
TCTGCCTATG GCTCTCTTTA CCCTCTCTTA CCTGTATATT ATAAACAGCT GGGAATGTCA 
CCTAGCCAGA GTGGACTGTT GGTGGGCATT CGATACTTCA TTGAATTCTG CAGTGCCCCC 
TTCTGGGGTG TAGTTGCAGA TCGTTTCAGA AAGGGCAAAA TTGTCCTCCT CTTTTCGCTT 
CTGTGTTGGG TTTTGTTCAA CCTGGGCATT GGATTTGTCA AACCTGCTAC CTTGAGATGT 
CTACCAAAGA TCCCCCCAAC AGCTCACCCC ACCAATGTAA GTCACCCAGT AACTGTTCTG 
CCAATGAACT CCTCCACTGT CGCTTTCTTT AGCACACCAC CAAAATTGCT CCAGAAAAGG 
GACGTGCAAT TGTCTGAAAC AGAGCCCAAT ATCTCAGACA TAGATCTCGT GTCAACAGCA 
TTGACCTTGA CCAGTGAACC CACACGGAGG CCCCAGACAG AGGCAATTAC CCACCCTGTT 
ACAGGCTTGA TTTTGAACAC AAGCACAGTG ACTCTGCCCC CCACAGGAAA TGTCACCAGG 
GAGACAACCA TTGCTGTGGT CACCACCACC AAATCTTTAC CATCTGATCA AGTCACCCTT 
GTGTATGACC AACAGGAAGT TGAGGCGATA TTCTTAATTA TCTTAGTGGT TGTCATAATA 
GGAGAATTTT TCAGTGCCTC TTCTGTCACG ATTGTAGATA CAGTAACCCT CCAGTACCTG 
GGAAAACACC GAGACCGATA TGGGCTACAG CGCATGTGGG GCTCCCTGGG CTGGGGCCTG 
GCCATGCTGT CTGTGGGTAT TGGAATCGAC TATACTCATA TAGATGTGCT CATTGATGGG 
AAGGGGTGTA AGCCCCCTGA GTACCGGAAC TACCAGATTG TCTTCATCGT CTTTGGAGTT 
CTCATGACCA TGGCCCTGAT CGTTGCCACT CAGTTCCGGT TCCGCTACAA CCACTTCAAC 
AATAGTGATG GTAAAGGGAA AGAGGTGGAG ATCCCACAGG TGGAAAGGGA CAACTCCACA 
GAGTCCTCTG AAGAGACTCC AACTGCCGCG ACTCACTCAC AGGCCTTCAA CTTCTGGGAC 
TTGATCAAGT TGCTATGCAG TGTGCAGTAT GGCTCGGTTC TGTTTGTGGC CTGGTTCATG 
GGTTTTGGAT ACGGCTTCGT GTTCACCTTT CTCTACTGGC ATCTGGAAGA CCTAAATGGA 
ACTACAACCC TCTTTGGGGT CTGTTCAGTC CTGAGTCATG TGTCCGAGTT GACCGCTTAC 
TTCTTCAGCC ACAAGCTTAT TGAGTTGATT GGCCATATCA GGGTTTTATA CATTGGCCTG 
GCCTGTAATA CAGCTCGCTA TATTTACATT TCCTACCTGG AAAATGCCTG GACTGTCCTC 
CCTATGGAAG TTCTTCAAGG TGTCACACAC GCAGCCATCT GGGCAGCGTG CATCTCTTAC 
CTCAGTGCAG CTGTCCCCCC TGAGCTGAGG ACTTCTGCAC AGGGAATCCT GCAAGGCCTT 
CATCTGGGTC TGGGGCGAGG ATGTGGTGCC ATGATCGGAG GCGTGTTAGT CAATTATTTT 
GGGGCTGCTG CAACTTTCCG AGGGATTGGC ATGGCTTGCC TGGTGATCCT TCTCCTCTTT 
GCCCTGATCC AGTGGCTGGC AGTACCAGAT GAAGAAGAAG ACAAGACAAT GTTGGCAGAA 
AGGATCCCAG TTCCCTCTAG TCCCGTTCCC ATAGCAACCA TTGACCTGGT ACAGCAACAG 
ACAGAAGATG TCATGCCACG CGTTGAGGCC AGACTTCCGC CCAAGAAAAC CAAGCACCAG 
GAAGAGCAGG AAGATGTAAA TAAACCGGCC TGGGGGGTCA GCTCCTCGCC TTGGGTGACC 
TTTGTCTATG CTCTCTACCA AGTTAAAGAG CTAATTCAAC TGACAAGAGA GAGCCGTGCT 
TCTGAGATAC AACCTTTACA GGGGACCAGT GAGAATCGGG AAGCCTCTCC AGCTGGTGGA 
GCCCAGCGTG CCCCACGTGA AACTCACTCC GCCTCACCTA GAAACCAGCC ATCCCCTGAC 
ACAGCAGCAT CTCAAACGCA GTCCAGCCCT GCTCACCCCA GTGCTACCCC GCATGGACAG 
GAGAGTGGAG AGAAGCAGGC TCAGCCCACT GTGGGAGAAC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mfsd6 ( NM_001357464.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001344393.1 Mus musculus major facilitator superfamily domain containing 6 (Mfsd6), transcript variant 3, mRNA.

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CloneID	OMu27685
Clone ID Related Accession (Same CDS sequence)	XM_006496375.2 , NM_001357464.1
Accession Version	NM_001357464.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Mus musculus(house mouse)
Product	major facilitator superfamily domain-containing protein 6 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC139299.3 and AC161876.3. On Oct 28, 2017 this sequence version replaced XM_006496375.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.266472.1, SRR1660813.39222.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGCTG ATGATAAAGT TGCTATCCTA ACTGATGATG AAGAGGAGCA GAAGAGAAAA 
TATGTGCTTG CTGATCCCTT CAATGGCATC TGCAGGGAAC CAGAACCACC TTCTAATGAA 
ACCCCCTCCT CCACAGAGAC CTCAGCCATT CCCGAGGAGG AAATAGACTG GATAGAGAAA 
CATTGTGTGA AAGTCAACAA TGACCTTCTC ATCTCCAAGG TGTTTTATTT TTTCTTTTAC 
TCTGCCTATG GCTCTCTTTA CCCTCTCTTA CCTGTATATT ATAAACAGCT GGGAATGTCA 
CCTAGCCAGA GTGGACTGTT GGTGGGCATT CGATACTTCA TTGAATTCTG CAGTGCCCCC 
TTCTGGGGTG TAGTTGCAGA TCGTTTCAGA AAGGGCAAAA TTGTCCTCCT CTTTTCGCTT 
CTGTGTTGGG TTTTGTTCAA CCTGGGCATT GGATTTGTCA AACCTGCTAC CTTGAGATGT 
CTACCAAAGA TCCCCCCAAC AGCTCACCCC ACCAATGTAA GTCACCCAGT AACTGTTCTG 
CCAATGAACT CCTCCACTGT CGCTTTCTTT AGCACACCAC CAAAATTGCT CCAGAAAAGG 
GACGTGCAAT TGTCTGAAAC AGAGCCCAAT ATCTCAGACA TAGATCTCGT GTCAACAGCA 
TTGACCTTGA CCAGTGAACC CACACGGAGG CCCCAGACAG AGGCAATTAC CCACCCTGTT 
ACAGGCTTGA TTTTGAACAC AAGCACAGTG ACTCTGCCCC CCACAGGAAA TGTCACCAGG 
GAGACAACCA TTGCTGTGGT CACCACCACC AAATCTTTAC CATCTGATCA AGTCACCCTT 
GTGTATGACC AACAGGAAGT TGAGGCGATA TTCTTAATTA TCTTAGTGGT TGTCATAATA 
GGAGAATTTT TCAGTGCCTC TTCTGTCACG ATTGTAGATA CAGTAACCCT CCAGTACCTG 
GGAAAACACC GAGACCGATA TGGGCTACAG CGCATGTGGG GCTCCCTGGG CTGGGGCCTG 
GCCATGCTGT CTGTGGGTAT TGGAATCGAC TATACTCATA TAGATGTGCT CATTGATGGG 
AAGGGGTGTA AGCCCCCTGA GTACCGGAAC TACCAGATTG TCTTCATCGT CTTTGGAGTT 
CTCATGACCA TGGCCCTGAT CGTTGCCACT CAGTTCCGGT TCCGCTACAA CCACTTCAAC 
AATAGTGATG GTAAAGGGAA AGAGGTGGAG ATCCCACAGG TGGAAAGGGA CAACTCCACA 
GAGTCCTCTG AAGAGACTCC AACTGCCGCG ACTCACTCAC AGGCCTTCAA CTTCTGGGAC 
TTGATCAAGT TGCTATGCAG TGTGCAGTAT GGCTCGGTTC TGTTTGTGGC CTGGTTCATG 
GGTTTTGGAT ACGGCTTCGT GTTCACCTTT CTCTACTGGC ATCTGGAAGA CCTAAATGGA 
ACTACAACCC TCTTTGGGGT CTGTTCAGTC CTGAGTCATG TGTCCGAGTT GACCGCTTAC 
TTCTTCAGCC ACAAGCTTAT TGAGTTGATT GGCCATATCA GGGTTTTATA CATTGGCCTG 
GCCTGTAATA CAGCTCGCTA TATTTACATT TCCTACCTGG AAAATGCCTG GACTGTCCTC 
CCTATGGAAG TTCTTCAAGG TGTCACACAC GCAGCCATCT GGGCAGCGTG CATCTCTTAC 
CTCAGTGCAG CTGTCCCCCC TGAGCTGAGG ACTTCTGCAC AGGGAATCCT GCAAGGCCTT 
CATCTGGGTC TGGGGCGAGG ATGTGGTGCC ATGATCGGAG GCGTGTTAGT CAATTATTTT 
GGGGCTGCTG CAACTTTCCG AGGGATTGGC ATGGCTTGCC TGGTGATCCT TCTCCTCTTT 
GCCCTGATCC AGTGGCTGGC AGTACCAGAT GAAGAAGAAG ACAAGACAAT GTTGGCAGAA 
AGGATCCCAG TTCCCTCTAG TCCCGTTCCC ATAGCAACCA TTGACCTGGT ACAGCAACAG 
ACAGAAGATG TCATGCCACG CGTTGAGGCC AGACTTCCGC CCAAGAAAAC CAAGCACCAG 
GAAGAGCAGG AAGATGTAAA TAAACCGGCC TGGGGGGTCA GCTCCTCGCC TTGGGTGACC 
TTTGTCTATG CTCTCTACCA AGTTAAAGAG CTAATTCAAC TGACAAGAGA GAGCCGTGCT 
TCTGAGATAC AACCTTTACA GTTGCACATA GGAAAGGAGA TGAAAGGGGA CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001344393.1
CDS	388..2604
Translation	MAADDKVAILTDDEEEQKRKYVLADPFNGICREPEPPSNETPSSTETSAIPEEEIDWIEKHCVKVNNDLLISKVFYFFFYSAYGSLYPLLPVYYKQLGMSPSQSGLLVGIRYFIEFCSAPFWGVVADRFRKGKIVLLFSLLCWVLFNLGIGFVKPATLRCLPKIPPTAHPTNVSHPVTVLPMNSSTVAFFSTPPKLLQKRDVQLSETEPNISDIDLVSTALTLTSEPTRRPQTEAITHPVTGLILNTSTVTLPPTGNVTRETTIAVVTTTKSLPSDQVTLVYDQQEVEAIFLIILVVVIIGEFFSASSVTIVDTVTLQYLGKHRDRYGLQRMWGSLGWGLAMLSVGIGIDYTHIDVLIDGKGCKPPEYRNYQIVFIVFGVLMTMALIVATQFRFRYNHFNNSDGKGKEVEIPQVERDNSTESSEETPTAATHSQAFNFWDLIKLLCSVQYGSVLFVAWFMGFGYGFVFTFLYWHLEDLNGTTTLFGVCSVLSHVSELTAYFFSHKLIELIGHIRVLYIGLACNTARYIYISYLENAWTVLPMEVLQGVTHAAIWAACISYLSAAVPPELRTSAQGILQGLHLGLGRGCGAMIGGVLVNYFGAAATFRGIGMACLVILLLFALIQWLAVPDEEEDKTMLAERIPVPSSPVPIATIDLVQQQTEDVMPRVEARLPPKKTKHQEEQEDVNKPAWGVSSSPWVTFVYALYQVKELIQLTRESRASEIQPLQLHIGKEMKGDQ

Target ORF information:

RefSeq Version	NM_001357464.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus major facilitator superfamily domain containing 6 (Mfsd6), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001357464.1

ORF Insert Sequence:

ATGGCTGCTG ATGATAAAGT TGCTATCCTA ACTGATGATG AAGAGGAGCA GAAGAGAAAA 
TATGTGCTTG CTGATCCCTT CAATGGCATC TGCAGGGAAC CAGAACCACC TTCTAATGAA 
ACCCCCTCCT CCACAGAGAC CTCAGCCATT CCCGAGGAGG AAATAGACTG GATAGAGAAA 
CATTGTGTGA AAGTCAACAA TGACCTTCTC ATCTCCAAGG TGTTTTATTT TTTCTTTTAC 
TCTGCCTATG GCTCTCTTTA CCCTCTCTTA CCTGTATATT ATAAACAGCT GGGAATGTCA 
CCTAGCCAGA GTGGACTGTT GGTGGGCATT CGATACTTCA TTGAATTCTG CAGTGCCCCC 
TTCTGGGGTG TAGTTGCAGA TCGTTTCAGA AAGGGCAAAA TTGTCCTCCT CTTTTCGCTT 
CTGTGTTGGG TTTTGTTCAA CCTGGGCATT GGATTTGTCA AACCTGCTAC CTTGAGATGT 
CTACCAAAGA TCCCCCCAAC AGCTCACCCC ACCAATGTAA GTCACCCAGT AACTGTTCTG 
CCAATGAACT CCTCCACTGT CGCTTTCTTT AGCACACCAC CAAAATTGCT CCAGAAAAGG 
GACGTGCAAT TGTCTGAAAC AGAGCCCAAT ATCTCAGACA TAGATCTCGT GTCAACAGCA 
TTGACCTTGA CCAGTGAACC CACACGGAGG CCCCAGACAG AGGCAATTAC CCACCCTGTT 
ACAGGCTTGA TTTTGAACAC AAGCACAGTG ACTCTGCCCC CCACAGGAAA TGTCACCAGG 
GAGACAACCA TTGCTGTGGT CACCACCACC AAATCTTTAC CATCTGATCA AGTCACCCTT 
GTGTATGACC AACAGGAAGT TGAGGCGATA TTCTTAATTA TCTTAGTGGT TGTCATAATA 
GGAGAATTTT TCAGTGCCTC TTCTGTCACG ATTGTAGATA CAGTAACCCT CCAGTACCTG 
GGAAAACACC GAGACCGATA TGGGCTACAG CGCATGTGGG GCTCCCTGGG CTGGGGCCTG 
GCCATGCTGT CTGTGGGTAT TGGAATCGAC TATACTCATA TAGATGTGCT CATTGATGGG 
AAGGGGTGTA AGCCCCCTGA GTACCGGAAC TACCAGATTG TCTTCATCGT CTTTGGAGTT 
CTCATGACCA TGGCCCTGAT CGTTGCCACT CAGTTCCGGT TCCGCTACAA CCACTTCAAC 
AATAGTGATG GTAAAGGGAA AGAGGTGGAG ATCCCACAGG TGGAAAGGGA CAACTCCACA 
GAGTCCTCTG AAGAGACTCC AACTGCCGCG ACTCACTCAC AGGCCTTCAA CTTCTGGGAC 
TTGATCAAGT TGCTATGCAG TGTGCAGTAT GGCTCGGTTC TGTTTGTGGC CTGGTTCATG 
GGTTTTGGAT ACGGCTTCGT GTTCACCTTT CTCTACTGGC ATCTGGAAGA CCTAAATGGA 
ACTACAACCC TCTTTGGGGT CTGTTCAGTC CTGAGTCATG TGTCCGAGTT GACCGCTTAC 
TTCTTCAGCC ACAAGCTTAT TGAGTTGATT GGCCATATCA GGGTTTTATA CATTGGCCTG 
GCCTGTAATA CAGCTCGCTA TATTTACATT TCCTACCTGG AAAATGCCTG GACTGTCCTC 
CCTATGGAAG TTCTTCAAGG TGTCACACAC GCAGCCATCT GGGCAGCGTG CATCTCTTAC 
CTCAGTGCAG CTGTCCCCCC TGAGCTGAGG ACTTCTGCAC AGGGAATCCT GCAAGGCCTT 
CATCTGGGTC TGGGGCGAGG ATGTGGTGCC ATGATCGGAG GCGTGTTAGT CAATTATTTT 
GGGGCTGCTG CAACTTTCCG AGGGATTGGC ATGGCTTGCC TGGTGATCCT TCTCCTCTTT 
GCCCTGATCC AGTGGCTGGC AGTACCAGAT GAAGAAGAAG ACAAGACAAT GTTGGCAGAA 
AGGATCCCAG TTCCCTCTAG TCCCGTTCCC ATAGCAACCA TTGACCTGGT ACAGCAACAG 
ACAGAAGATG TCATGCCACG CGTTGAGGCC AGACTTCCGC CCAAGAAAAC CAAGCACCAG 
GAAGAGCAGG AAGATGTAAA TAAACCGGCC TGGGGGGTCA GCTCCTCGCC TTGGGTGACC 
TTTGTCTATG CTCTCTACCA AGTTAAAGAG CTAATTCAAC TGACAAGAGA GAGCCGTGCT 
TCTGAGATAC AACCTTTACA GTTGCACATA GGAAAGGAGA TGAAAGGGGA CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mfsd6 ( XM_006496374.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496437.1 Mus musculus major facilitator superfamily domain containing 6 (Mfsd6), transcript variant X1, mRNA.

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CloneID	OMu10260
Clone ID Related Accession (Same CDS sequence)	XM_006496374.3 , NM_133829.2 , XM_006496374.4
Accession Version	XM_006496374.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	major facilitator superfamily domain-containing protein 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496374.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG ATGATAAAGT TGCTATCCTA ACTGATGATG AAGAGGAGCA GAAGAGAAAA 
TATGTGCTTG CTGATCCCTT CAATGGCATC TGCAGGGAAC CAGAACCACC TTCTAATGAA 
ACCCCCTCCT CCACAGAGAC CTCAGCCATT CCCGAGGAGG AAATAGACTG GATAGAGAAA 
CATTGTGTGA AAGTCAACAA TGACCTTCTC ATCTCCAAGG TGTTTTATTT TTTCTTTTAC 
TCTGCCTATG GCTCTCTTTA CCCTCTCTTA CCTGTATATT ATAAACAGCT GGGAATGTCA 
CCTAGCCAGA GTGGACTGTT GGTGGGCATT CGATACTTCA TTGAATTCTG CAGTGCCCCC 
TTCTGGGGTG TAGTTGCAGA TCGTTTCAGA AAGGGCAAAA TTGTCCTCCT CTTTTCGCTT 
CTGTGTTGGG TTTTGTTCAA CCTGGGCATT GGATTTGTCA AACCTGCTAC CTTGAGATGT 
CTACCAAAGA TCCCCCCAAC AGCTCACCCC ACCAATGTAA GTCACCCAGT AACTGTTCTG 
CCAATGAACT CCTCCACTGT CGCTTTCTTT AGCACACCAC CAAAATTGCT CCAGAAAAGG 
GACGTGCAAT TGTCTGAAAC AGAGCCCAAT ATCTCAGACA TAGATCTCGT GTCAACAGCA 
TTGACCTTGA CCAGTGAACC CACACGGAGG CCCCAGACAG AGGCAATTAC CCACCCTGTT 
ACAGGCTTGA TTTTGAACAC AAGCACAGTG ACTCTGCCCC CCACAGGAAA TGTCACCAGG 
GAGACAACCA TTGCTGTGGT CACCACCACC AAATCTTTAC CATCTGATCA AGTCACCCTT 
GTGTATGACC AACAGGAAGT TGAGGCGATA TTCTTAATTA TCTTAGTGGT TGTCATAATA 
GGAGAATTTT TCAGTGCCTC TTCTGTCACG ATTGTAGATA CAGTAACCCT CCAGTACCTG 
GGAAAACACC GAGACCGATA TGGGCTACAG CGCATGTGGG GCTCCCTGGG CTGGGGCCTG 
GCCATGCTGT CTGTGGGTAT TGGAATCGAC TATACTCATA TAGATGTGCT CATTGATGGG 
AAGGGGTGTA AGCCCCCTGA GTACCGGAAC TACCAGATTG TCTTCATCGT CTTTGGAGTT 
CTCATGACCA TGGCCCTGAT CGTTGCCACT CAGTTCCGGT TCCGCTACAA CCACTTCAAC 
AATAGTGATG GTAAAGGGAA AGAGGTGGAG ATCCCACAGG TGGAAAGGGA CAACTCCACA 
GAGTCCTCTG AAGAGACTCC AACTGCCGCG ACTCACTCAC AGGCCTTCAA CTTCTGGGAC 
TTGATCAAGT TGCTATGCAG TGTGCAGTAT GGCTCGGTTC TGTTTGTGGC CTGGTTCATG 
GGTTTTGGAT ACGGCTTCGT GTTCACCTTT CTCTACTGGC ATCTGGAAGA CCTAAATGGA 
ACTACAACCC TCTTTGGGGT CTGTTCAGTC CTGAGTCATG TGTCCGAGTT GACCGCTTAC 
TTCTTCAGCC ACAAGCTTAT TGAGTTGATT GGCCATATCA GGGTTTTATA CATTGGCCTG 
GCCTGTAATA CAGCTCGCTA TATTTACATT TCCTACCTGG AAAATGCCTG GACTGTCCTC 
CCTATGGAAG TTCTTCAAGG TGTCACACAC GCAGCCATCT GGGCAGCGTG CATCTCTTAC 
CTCAGTGCAG CTGTCCCCCC TGAGCTGAGG ACTTCTGCAC AGGGAATCCT GCAAGGCCTT 
CATCTGGGTC TGGGGCGAGG ATGTGGTGCC ATGATCGGAG GCGTGTTAGT CAATTATTTT 
GGGGCTGCTG CAACTTTCCG AGGGATTGGC ATGGCTTGCC TGGTGATCCT TCTCCTCTTT 
GCCCTGATCC AGTGGCTGGC AGTACCAGAT GAAGAAGAAG ACAAGACAAT GTTGGCAGAA 
AGGATCCCAG TTCCCTCTAG TCCCGTTCCC ATAGCAACCA TTGACCTGGT ACAGCAACAG 
ACAGAAGATG TCATGCCACG CGTTGAGGCC AGACTTCCGC CCAAGAAAAC CAAGCACCAG 
GAAGAGCAGG AAGATGTAAA TAAACCGGCC TGGGGGGTCA GCTCCTCGCC TTGGGTGACC 
TTTGTCTATG CTCTCTACCA AGTTAAAGAG CTAATTCAAC TGACAAGAGA GAGCCGTGCT 
TCTGAGATAC AACCTTTACA GGGGACCAGT GAGAATCGGG AAGCCTCTCC AGCTGGTGGA 
GCCCAGCGTG CCCCACGTGA AACTCACTCC GCCTCACCTA GAAACCAGCC ATCCCCTGAC 
ACAGCAGCAT CTCAAACGCA GTCCAGCCCT GCTCACCCCA GTGCTACCCC GCATGGACAG 
GAGAGTGGAG AGAAGCAGGC TCAGCCCACT GTGGGAGAAC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496437.1
CDS	304..2688
Translation	MAADDKVAILTDDEEEQKRKYVLADPFNGICREPEPPSNETPSSTETSAIPEEEIDWIEKHCVKVNNDLLISKVFYFFFYSAYGSLYPLLPVYYKQLGMSPSQSGLLVGIRYFIEFCSAPFWGVVADRFRKGKIVLLFSLLCWVLFNLGIGFVKPATLRCLPKIPPTAHPTNVSHPVTVLPMNSSTVAFFSTPPKLLQKRDVQLSETEPNISDIDLVSTALTLTSEPTRRPQTEAITHPVTGLILNTSTVTLPPTGNVTRETTIAVVTTTKSLPSDQVTLVYDQQEVEAIFLIILVVVIIGEFFSASSVTIVDTVTLQYLGKHRDRYGLQRMWGSLGWGLAMLSVGIGIDYTHIDVLIDGKGCKPPEYRNYQIVFIVFGVLMTMALIVATQFRFRYNHFNNSDGKGKEVEIPQVERDNSTESSEETPTAATHSQAFNFWDLIKLLCSVQYGSVLFVAWFMGFGYGFVFTFLYWHLEDLNGTTTLFGVCSVLSHVSELTAYFFSHKLIELIGHIRVLYIGLACNTARYIYISYLENAWTVLPMEVLQGVTHAAIWAACISYLSAAVPPELRTSAQGILQGLHLGLGRGCGAMIGGVLVNYFGAAATFRGIGMACLVILLLFALIQWLAVPDEEEDKTMLAERIPVPSSPVPIATIDLVQQQTEDVMPRVEARLPPKKTKHQEEQEDVNKPAWGVSSSPWVTFVYALYQVKELIQLTRESRASEIQPLQGTSENREASPAGGAQRAPRETHSASPRNQPSPDTAASQTQSSPAHPSATPHGQESGEKQAQPTVGEH

Target ORF information:

RefSeq Version	XM_006496374.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus major facilitator superfamily domain containing 6 (Mfsd6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496374.3

ORF Insert Sequence:

ATGGCTGCTG ATGATAAAGT TGCTATCCTA ACTGATGATG AAGAGGAGCA GAAGAGAAAA 
TATGTGCTTG CTGATCCCTT CAATGGCATC TGCAGGGAAC CAGAACCACC TTCTAATGAA 
ACCCCCTCCT CCACAGAGAC CTCAGCCATT CCCGAGGAGG AAATAGACTG GATAGAGAAA 
CATTGTGTGA AAGTCAACAA TGACCTTCTC ATCTCCAAGG TGTTTTATTT TTTCTTTTAC 
TCTGCCTATG GCTCTCTTTA CCCTCTCTTA CCTGTATATT ATAAACAGCT GGGAATGTCA 
CCTAGCCAGA GTGGACTGTT GGTGGGCATT CGATACTTCA TTGAATTCTG CAGTGCCCCC 
TTCTGGGGTG TAGTTGCAGA TCGTTTCAGA AAGGGCAAAA TTGTCCTCCT CTTTTCGCTT 
CTGTGTTGGG TTTTGTTCAA CCTGGGCATT GGATTTGTCA AACCTGCTAC CTTGAGATGT 
CTACCAAAGA TCCCCCCAAC AGCTCACCCC ACCAATGTAA GTCACCCAGT AACTGTTCTG 
CCAATGAACT CCTCCACTGT CGCTTTCTTT AGCACACCAC CAAAATTGCT CCAGAAAAGG 
GACGTGCAAT TGTCTGAAAC AGAGCCCAAT ATCTCAGACA TAGATCTCGT GTCAACAGCA 
TTGACCTTGA CCAGTGAACC CACACGGAGG CCCCAGACAG AGGCAATTAC CCACCCTGTT 
ACAGGCTTGA TTTTGAACAC AAGCACAGTG ACTCTGCCCC CCACAGGAAA TGTCACCAGG 
GAGACAACCA TTGCTGTGGT CACCACCACC AAATCTTTAC CATCTGATCA AGTCACCCTT 
GTGTATGACC AACAGGAAGT TGAGGCGATA TTCTTAATTA TCTTAGTGGT TGTCATAATA 
GGAGAATTTT TCAGTGCCTC TTCTGTCACG ATTGTAGATA CAGTAACCCT CCAGTACCTG 
GGAAAACACC GAGACCGATA TGGGCTACAG CGCATGTGGG GCTCCCTGGG CTGGGGCCTG 
GCCATGCTGT CTGTGGGTAT TGGAATCGAC TATACTCATA TAGATGTGCT CATTGATGGG 
AAGGGGTGTA AGCCCCCTGA GTACCGGAAC TACCAGATTG TCTTCATCGT CTTTGGAGTT 
CTCATGACCA TGGCCCTGAT CGTTGCCACT CAGTTCCGGT TCCGCTACAA CCACTTCAAC 
AATAGTGATG GTAAAGGGAA AGAGGTGGAG ATCCCACAGG TGGAAAGGGA CAACTCCACA 
GAGTCCTCTG AAGAGACTCC AACTGCCGCG ACTCACTCAC AGGCCTTCAA CTTCTGGGAC 
TTGATCAAGT TGCTATGCAG TGTGCAGTAT GGCTCGGTTC TGTTTGTGGC CTGGTTCATG 
GGTTTTGGAT ACGGCTTCGT GTTCACCTTT CTCTACTGGC ATCTGGAAGA CCTAAATGGA 
ACTACAACCC TCTTTGGGGT CTGTTCAGTC CTGAGTCATG TGTCCGAGTT GACCGCTTAC 
TTCTTCAGCC ACAAGCTTAT TGAGTTGATT GGCCATATCA GGGTTTTATA CATTGGCCTG 
GCCTGTAATA CAGCTCGCTA TATTTACATT TCCTACCTGG AAAATGCCTG GACTGTCCTC 
CCTATGGAAG TTCTTCAAGG TGTCACACAC GCAGCCATCT GGGCAGCGTG CATCTCTTAC 
CTCAGTGCAG CTGTCCCCCC TGAGCTGAGG ACTTCTGCAC AGGGAATCCT GCAAGGCCTT 
CATCTGGGTC TGGGGCGAGG ATGTGGTGCC ATGATCGGAG GCGTGTTAGT CAATTATTTT 
GGGGCTGCTG CAACTTTCCG AGGGATTGGC ATGGCTTGCC TGGTGATCCT TCTCCTCTTT 
GCCCTGATCC AGTGGCTGGC AGTACCAGAT GAAGAAGAAG ACAAGACAAT GTTGGCAGAA 
AGGATCCCAG TTCCCTCTAG TCCCGTTCCC ATAGCAACCA TTGACCTGGT ACAGCAACAG 
ACAGAAGATG TCATGCCACG CGTTGAGGCC AGACTTCCGC CCAAGAAAAC CAAGCACCAG 
GAAGAGCAGG AAGATGTAAA TAAACCGGCC TGGGGGGTCA GCTCCTCGCC TTGGGTGACC 
TTTGTCTATG CTCTCTACCA AGTTAAAGAG CTAATTCAAC TGACAAGAGA GAGCCGTGCT 
TCTGAGATAC AACCTTTACA GGGGACCAGT GAGAATCGGG AAGCCTCTCC AGCTGGTGGA 
GCCCAGCGTG CCCCACGTGA AACTCACTCC GCCTCACCTA GAAACCAGCC ATCCCCTGAC 
ACAGCAGCAT CTCAAACGCA GTCCAGCCCT GCTCACCCCA GTGCTACCCC GCATGGACAG 
GAGAGTGGAG AGAAGCAGGC TCAGCCCACT GTGGGAGAAC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mfsd6 ( XM_006496375.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496438.1 Mus musculus major facilitator superfamily domain containing 6 (Mfsd6), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu27685
Clone ID Related Accession (Same CDS sequence)	XM_006496375.2 , NM_001357464.1
Accession Version	XM_006496375.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	major facilitator superfamily domain-containing protein 6 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496375.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG ATGATAAAGT TGCTATCCTA ACTGATGATG AAGAGGAGCA GAAGAGAAAA 
TATGTGCTTG CTGATCCCTT CAATGGCATC TGCAGGGAAC CAGAACCACC TTCTAATGAA 
ACCCCCTCCT CCACAGAGAC CTCAGCCATT CCCGAGGAGG AAATAGACTG GATAGAGAAA 
CATTGTGTGA AAGTCAACAA TGACCTTCTC ATCTCCAAGG TGTTTTATTT TTTCTTTTAC 
TCTGCCTATG GCTCTCTTTA CCCTCTCTTA CCTGTATATT ATAAACAGCT GGGAATGTCA 
CCTAGCCAGA GTGGACTGTT GGTGGGCATT CGATACTTCA TTGAATTCTG CAGTGCCCCC 
TTCTGGGGTG TAGTTGCAGA TCGTTTCAGA AAGGGCAAAA TTGTCCTCCT CTTTTCGCTT 
CTGTGTTGGG TTTTGTTCAA CCTGGGCATT GGATTTGTCA AACCTGCTAC CTTGAGATGT 
CTACCAAAGA TCCCCCCAAC AGCTCACCCC ACCAATGTAA GTCACCCAGT AACTGTTCTG 
CCAATGAACT CCTCCACTGT CGCTTTCTTT AGCACACCAC CAAAATTGCT CCAGAAAAGG 
GACGTGCAAT TGTCTGAAAC AGAGCCCAAT ATCTCAGACA TAGATCTCGT GTCAACAGCA 
TTGACCTTGA CCAGTGAACC CACACGGAGG CCCCAGACAG AGGCAATTAC CCACCCTGTT 
ACAGGCTTGA TTTTGAACAC AAGCACAGTG ACTCTGCCCC CCACAGGAAA TGTCACCAGG 
GAGACAACCA TTGCTGTGGT CACCACCACC AAATCTTTAC CATCTGATCA AGTCACCCTT 
GTGTATGACC AACAGGAAGT TGAGGCGATA TTCTTAATTA TCTTAGTGGT TGTCATAATA 
GGAGAATTTT TCAGTGCCTC TTCTGTCACG ATTGTAGATA CAGTAACCCT CCAGTACCTG 
GGAAAACACC GAGACCGATA TGGGCTACAG CGCATGTGGG GCTCCCTGGG CTGGGGCCTG 
GCCATGCTGT CTGTGGGTAT TGGAATCGAC TATACTCATA TAGATGTGCT CATTGATGGG 
AAGGGGTGTA AGCCCCCTGA GTACCGGAAC TACCAGATTG TCTTCATCGT CTTTGGAGTT 
CTCATGACCA TGGCCCTGAT CGTTGCCACT CAGTTCCGGT TCCGCTACAA CCACTTCAAC 
AATAGTGATG GTAAAGGGAA AGAGGTGGAG ATCCCACAGG TGGAAAGGGA CAACTCCACA 
GAGTCCTCTG AAGAGACTCC AACTGCCGCG ACTCACTCAC AGGCCTTCAA CTTCTGGGAC 
TTGATCAAGT TGCTATGCAG TGTGCAGTAT GGCTCGGTTC TGTTTGTGGC CTGGTTCATG 
GGTTTTGGAT ACGGCTTCGT GTTCACCTTT CTCTACTGGC ATCTGGAAGA CCTAAATGGA 
ACTACAACCC TCTTTGGGGT CTGTTCAGTC CTGAGTCATG TGTCCGAGTT GACCGCTTAC 
TTCTTCAGCC ACAAGCTTAT TGAGTTGATT GGCCATATCA GGGTTTTATA CATTGGCCTG 
GCCTGTAATA CAGCTCGCTA TATTTACATT TCCTACCTGG AAAATGCCTG GACTGTCCTC 
CCTATGGAAG TTCTTCAAGG TGTCACACAC GCAGCCATCT GGGCAGCGTG CATCTCTTAC 
CTCAGTGCAG CTGTCCCCCC TGAGCTGAGG ACTTCTGCAC AGGGAATCCT GCAAGGCCTT 
CATCTGGGTC TGGGGCGAGG ATGTGGTGCC ATGATCGGAG GCGTGTTAGT CAATTATTTT 
GGGGCTGCTG CAACTTTCCG AGGGATTGGC ATGGCTTGCC TGGTGATCCT TCTCCTCTTT 
GCCCTGATCC AGTGGCTGGC AGTACCAGAT GAAGAAGAAG ACAAGACAAT GTTGGCAGAA 
AGGATCCCAG TTCCCTCTAG TCCCGTTCCC ATAGCAACCA TTGACCTGGT ACAGCAACAG 
ACAGAAGATG TCATGCCACG CGTTGAGGCC AGACTTCCGC CCAAGAAAAC CAAGCACCAG 
GAAGAGCAGG AAGATGTAAA TAAACCGGCC TGGGGGGTCA GCTCCTCGCC TTGGGTGACC 
TTTGTCTATG CTCTCTACCA AGTTAAAGAG CTAATTCAAC TGACAAGAGA GAGCCGTGCT 
TCTGAGATAC AACCTTTACA GTTGCACATA GGAAAGGAGA TGAAAGGGGA CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496438.1
CDS	492..2708
Translation	MAADDKVAILTDDEEEQKRKYVLADPFNGICREPEPPSNETPSSTETSAIPEEEIDWIEKHCVKVNNDLLISKVFYFFFYSAYGSLYPLLPVYYKQLGMSPSQSGLLVGIRYFIEFCSAPFWGVVADRFRKGKIVLLFSLLCWVLFNLGIGFVKPATLRCLPKIPPTAHPTNVSHPVTVLPMNSSTVAFFSTPPKLLQKRDVQLSETEPNISDIDLVSTALTLTSEPTRRPQTEAITHPVTGLILNTSTVTLPPTGNVTRETTIAVVTTTKSLPSDQVTLVYDQQEVEAIFLIILVVVIIGEFFSASSVTIVDTVTLQYLGKHRDRYGLQRMWGSLGWGLAMLSVGIGIDYTHIDVLIDGKGCKPPEYRNYQIVFIVFGVLMTMALIVATQFRFRYNHFNNSDGKGKEVEIPQVERDNSTESSEETPTAATHSQAFNFWDLIKLLCSVQYGSVLFVAWFMGFGYGFVFTFLYWHLEDLNGTTTLFGVCSVLSHVSELTAYFFSHKLIELIGHIRVLYIGLACNTARYIYISYLENAWTVLPMEVLQGVTHAAIWAACISYLSAAVPPELRTSAQGILQGLHLGLGRGCGAMIGGVLVNYFGAAATFRGIGMACLVILLLFALIQWLAVPDEEEDKTMLAERIPVPSSPVPIATIDLVQQQTEDVMPRVEARLPPKKTKHQEEQEDVNKPAWGVSSSPWVTFVYALYQVKELIQLTRESRASEIQPLQLHIGKEMKGDQ

Target ORF information:

RefSeq Version	XM_006496375.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus major facilitator superfamily domain containing 6 (Mfsd6), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496375.2

ORF Insert Sequence:

ATGGCTGCTG ATGATAAAGT TGCTATCCTA ACTGATGATG AAGAGGAGCA GAAGAGAAAA 
TATGTGCTTG CTGATCCCTT CAATGGCATC TGCAGGGAAC CAGAACCACC TTCTAATGAA 
ACCCCCTCCT CCACAGAGAC CTCAGCCATT CCCGAGGAGG AAATAGACTG GATAGAGAAA 
CATTGTGTGA AAGTCAACAA TGACCTTCTC ATCTCCAAGG TGTTTTATTT TTTCTTTTAC 
TCTGCCTATG GCTCTCTTTA CCCTCTCTTA CCTGTATATT ATAAACAGCT GGGAATGTCA 
CCTAGCCAGA GTGGACTGTT GGTGGGCATT CGATACTTCA TTGAATTCTG CAGTGCCCCC 
TTCTGGGGTG TAGTTGCAGA TCGTTTCAGA AAGGGCAAAA TTGTCCTCCT CTTTTCGCTT 
CTGTGTTGGG TTTTGTTCAA CCTGGGCATT GGATTTGTCA AACCTGCTAC CTTGAGATGT 
CTACCAAAGA TCCCCCCAAC AGCTCACCCC ACCAATGTAA GTCACCCAGT AACTGTTCTG 
CCAATGAACT CCTCCACTGT CGCTTTCTTT AGCACACCAC CAAAATTGCT CCAGAAAAGG 
GACGTGCAAT TGTCTGAAAC AGAGCCCAAT ATCTCAGACA TAGATCTCGT GTCAACAGCA 
TTGACCTTGA CCAGTGAACC CACACGGAGG CCCCAGACAG AGGCAATTAC CCACCCTGTT 
ACAGGCTTGA TTTTGAACAC AAGCACAGTG ACTCTGCCCC CCACAGGAAA TGTCACCAGG 
GAGACAACCA TTGCTGTGGT CACCACCACC AAATCTTTAC CATCTGATCA AGTCACCCTT 
GTGTATGACC AACAGGAAGT TGAGGCGATA TTCTTAATTA TCTTAGTGGT TGTCATAATA 
GGAGAATTTT TCAGTGCCTC TTCTGTCACG ATTGTAGATA CAGTAACCCT CCAGTACCTG 
GGAAAACACC GAGACCGATA TGGGCTACAG CGCATGTGGG GCTCCCTGGG CTGGGGCCTG 
GCCATGCTGT CTGTGGGTAT TGGAATCGAC TATACTCATA TAGATGTGCT CATTGATGGG 
AAGGGGTGTA AGCCCCCTGA GTACCGGAAC TACCAGATTG TCTTCATCGT CTTTGGAGTT 
CTCATGACCA TGGCCCTGAT CGTTGCCACT CAGTTCCGGT TCCGCTACAA CCACTTCAAC 
AATAGTGATG GTAAAGGGAA AGAGGTGGAG ATCCCACAGG TGGAAAGGGA CAACTCCACA 
GAGTCCTCTG AAGAGACTCC AACTGCCGCG ACTCACTCAC AGGCCTTCAA CTTCTGGGAC 
TTGATCAAGT TGCTATGCAG TGTGCAGTAT GGCTCGGTTC TGTTTGTGGC CTGGTTCATG 
GGTTTTGGAT ACGGCTTCGT GTTCACCTTT CTCTACTGGC ATCTGGAAGA CCTAAATGGA 
ACTACAACCC TCTTTGGGGT CTGTTCAGTC CTGAGTCATG TGTCCGAGTT GACCGCTTAC 
TTCTTCAGCC ACAAGCTTAT TGAGTTGATT GGCCATATCA GGGTTTTATA CATTGGCCTG 
GCCTGTAATA CAGCTCGCTA TATTTACATT TCCTACCTGG AAAATGCCTG GACTGTCCTC 
CCTATGGAAG TTCTTCAAGG TGTCACACAC GCAGCCATCT GGGCAGCGTG CATCTCTTAC 
CTCAGTGCAG CTGTCCCCCC TGAGCTGAGG ACTTCTGCAC AGGGAATCCT GCAAGGCCTT 
CATCTGGGTC TGGGGCGAGG ATGTGGTGCC ATGATCGGAG GCGTGTTAGT CAATTATTTT 
GGGGCTGCTG CAACTTTCCG AGGGATTGGC ATGGCTTGCC TGGTGATCCT TCTCCTCTTT 
GCCCTGATCC AGTGGCTGGC AGTACCAGAT GAAGAAGAAG ACAAGACAAT GTTGGCAGAA 
AGGATCCCAG TTCCCTCTAG TCCCGTTCCC ATAGCAACCA TTGACCTGGT ACAGCAACAG 
ACAGAAGATG TCATGCCACG CGTTGAGGCC AGACTTCCGC CCAAGAAAAC CAAGCACCAG 
GAAGAGCAGG AAGATGTAAA TAAACCGGCC TGGGGGGTCA GCTCCTCGCC TTGGGTGACC 
TTTGTCTATG CTCTCTACCA AGTTAAAGAG CTAATTCAAC TGACAAGAGA GAGCCGTGCT 
TCTGAGATAC AACCTTTACA GTTGCACATA GGAAAGGAGA TGAAAGGGGA CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mfsd6 ( XM_006496374.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496437.1 Mus musculus major facilitator superfamily domain containing 6 (Mfsd6), transcript variant X1, mRNA.

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CloneID	OMu10260
Clone ID Related Accession (Same CDS sequence)	XM_006496374.3 , NM_133829.2 , XM_006496374.4
Accession Version	XM_006496374.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	major facilitator superfamily domain-containing protein 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496374.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG ATGATAAAGT TGCTATCCTA ACTGATGATG AAGAGGAGCA GAAGAGAAAA 
TATGTGCTTG CTGATCCCTT CAATGGCATC TGCAGGGAAC CAGAACCACC TTCTAATGAA 
ACCCCCTCCT CCACAGAGAC CTCAGCCATT CCCGAGGAGG AAATAGACTG GATAGAGAAA 
CATTGTGTGA AAGTCAACAA TGACCTTCTC ATCTCCAAGG TGTTTTATTT TTTCTTTTAC 
TCTGCCTATG GCTCTCTTTA CCCTCTCTTA CCTGTATATT ATAAACAGCT GGGAATGTCA 
CCTAGCCAGA GTGGACTGTT GGTGGGCATT CGATACTTCA TTGAATTCTG CAGTGCCCCC 
TTCTGGGGTG TAGTTGCAGA TCGTTTCAGA AAGGGCAAAA TTGTCCTCCT CTTTTCGCTT 
CTGTGTTGGG TTTTGTTCAA CCTGGGCATT GGATTTGTCA AACCTGCTAC CTTGAGATGT 
CTACCAAAGA TCCCCCCAAC AGCTCACCCC ACCAATGTAA GTCACCCAGT AACTGTTCTG 
CCAATGAACT CCTCCACTGT CGCTTTCTTT AGCACACCAC CAAAATTGCT CCAGAAAAGG 
GACGTGCAAT TGTCTGAAAC AGAGCCCAAT ATCTCAGACA TAGATCTCGT GTCAACAGCA 
TTGACCTTGA CCAGTGAACC CACACGGAGG CCCCAGACAG AGGCAATTAC CCACCCTGTT 
ACAGGCTTGA TTTTGAACAC AAGCACAGTG ACTCTGCCCC CCACAGGAAA TGTCACCAGG 
GAGACAACCA TTGCTGTGGT CACCACCACC AAATCTTTAC CATCTGATCA AGTCACCCTT 
GTGTATGACC AACAGGAAGT TGAGGCGATA TTCTTAATTA TCTTAGTGGT TGTCATAATA 
GGAGAATTTT TCAGTGCCTC TTCTGTCACG ATTGTAGATA CAGTAACCCT CCAGTACCTG 
GGAAAACACC GAGACCGATA TGGGCTACAG CGCATGTGGG GCTCCCTGGG CTGGGGCCTG 
GCCATGCTGT CTGTGGGTAT TGGAATCGAC TATACTCATA TAGATGTGCT CATTGATGGG 
AAGGGGTGTA AGCCCCCTGA GTACCGGAAC TACCAGATTG TCTTCATCGT CTTTGGAGTT 
CTCATGACCA TGGCCCTGAT CGTTGCCACT CAGTTCCGGT TCCGCTACAA CCACTTCAAC 
AATAGTGATG GTAAAGGGAA AGAGGTGGAG ATCCCACAGG TGGAAAGGGA CAACTCCACA 
GAGTCCTCTG AAGAGACTCC AACTGCCGCG ACTCACTCAC AGGCCTTCAA CTTCTGGGAC 
TTGATCAAGT TGCTATGCAG TGTGCAGTAT GGCTCGGTTC TGTTTGTGGC CTGGTTCATG 
GGTTTTGGAT ACGGCTTCGT GTTCACCTTT CTCTACTGGC ATCTGGAAGA CCTAAATGGA 
ACTACAACCC TCTTTGGGGT CTGTTCAGTC CTGAGTCATG TGTCCGAGTT GACCGCTTAC 
TTCTTCAGCC ACAAGCTTAT TGAGTTGATT GGCCATATCA GGGTTTTATA CATTGGCCTG 
GCCTGTAATA CAGCTCGCTA TATTTACATT TCCTACCTGG AAAATGCCTG GACTGTCCTC 
CCTATGGAAG TTCTTCAAGG TGTCACACAC GCAGCCATCT GGGCAGCGTG CATCTCTTAC 
CTCAGTGCAG CTGTCCCCCC TGAGCTGAGG ACTTCTGCAC AGGGAATCCT GCAAGGCCTT 
CATCTGGGTC TGGGGCGAGG ATGTGGTGCC ATGATCGGAG GCGTGTTAGT CAATTATTTT 
GGGGCTGCTG CAACTTTCCG AGGGATTGGC ATGGCTTGCC TGGTGATCCT TCTCCTCTTT 
GCCCTGATCC AGTGGCTGGC AGTACCAGAT GAAGAAGAAG ACAAGACAAT GTTGGCAGAA 
AGGATCCCAG TTCCCTCTAG TCCCGTTCCC ATAGCAACCA TTGACCTGGT ACAGCAACAG 
ACAGAAGATG TCATGCCACG CGTTGAGGCC AGACTTCCGC CCAAGAAAAC CAAGCACCAG 
GAAGAGCAGG AAGATGTAAA TAAACCGGCC TGGGGGGTCA GCTCCTCGCC TTGGGTGACC 
TTTGTCTATG CTCTCTACCA AGTTAAAGAG CTAATTCAAC TGACAAGAGA GAGCCGTGCT 
TCTGAGATAC AACCTTTACA GGGGACCAGT GAGAATCGGG AAGCCTCTCC AGCTGGTGGA 
GCCCAGCGTG CCCCACGTGA AACTCACTCC GCCTCACCTA GAAACCAGCC ATCCCCTGAC 
ACAGCAGCAT CTCAAACGCA GTCCAGCCCT GCTCACCCCA GTGCTACCCC GCATGGACAG 
GAGAGTGGAG AGAAGCAGGC TCAGCCCACT GTGGGAGAAC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496437.1
CDS	372..2756
Translation	MAADDKVAILTDDEEEQKRKYVLADPFNGICREPEPPSNETPSSTETSAIPEEEIDWIEKHCVKVNNDLLISKVFYFFFYSAYGSLYPLLPVYYKQLGMSPSQSGLLVGIRYFIEFCSAPFWGVVADRFRKGKIVLLFSLLCWVLFNLGIGFVKPATLRCLPKIPPTAHPTNVSHPVTVLPMNSSTVAFFSTPPKLLQKRDVQLSETEPNISDIDLVSTALTLTSEPTRRPQTEAITHPVTGLILNTSTVTLPPTGNVTRETTIAVVTTTKSLPSDQVTLVYDQQEVEAIFLIILVVVIIGEFFSASSVTIVDTVTLQYLGKHRDRYGLQRMWGSLGWGLAMLSVGIGIDYTHIDVLIDGKGCKPPEYRNYQIVFIVFGVLMTMALIVATQFRFRYNHFNNSDGKGKEVEIPQVERDNSTESSEETPTAATHSQAFNFWDLIKLLCSVQYGSVLFVAWFMGFGYGFVFTFLYWHLEDLNGTTTLFGVCSVLSHVSELTAYFFSHKLIELIGHIRVLYIGLACNTARYIYISYLENAWTVLPMEVLQGVTHAAIWAACISYLSAAVPPELRTSAQGILQGLHLGLGRGCGAMIGGVLVNYFGAAATFRGIGMACLVILLLFALIQWLAVPDEEEDKTMLAERIPVPSSPVPIATIDLVQQQTEDVMPRVEARLPPKKTKHQEEQEDVNKPAWGVSSSPWVTFVYALYQVKELIQLTRESRASEIQPLQGTSENREASPAGGAQRAPRETHSASPRNQPSPDTAASQTQSSPAHPSATPHGQESGEKQAQPTVGEH

Target ORF information:

RefSeq Version	XM_006496374.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus major facilitator superfamily domain containing 6 (Mfsd6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496374.4

ORF Insert Sequence:

ATGGCTGCTG ATGATAAAGT TGCTATCCTA ACTGATGATG AAGAGGAGCA GAAGAGAAAA 
TATGTGCTTG CTGATCCCTT CAATGGCATC TGCAGGGAAC CAGAACCACC TTCTAATGAA 
ACCCCCTCCT CCACAGAGAC CTCAGCCATT CCCGAGGAGG AAATAGACTG GATAGAGAAA 
CATTGTGTGA AAGTCAACAA TGACCTTCTC ATCTCCAAGG TGTTTTATTT TTTCTTTTAC 
TCTGCCTATG GCTCTCTTTA CCCTCTCTTA CCTGTATATT ATAAACAGCT GGGAATGTCA 
CCTAGCCAGA GTGGACTGTT GGTGGGCATT CGATACTTCA TTGAATTCTG CAGTGCCCCC 
TTCTGGGGTG TAGTTGCAGA TCGTTTCAGA AAGGGCAAAA TTGTCCTCCT CTTTTCGCTT 
CTGTGTTGGG TTTTGTTCAA CCTGGGCATT GGATTTGTCA AACCTGCTAC CTTGAGATGT 
CTACCAAAGA TCCCCCCAAC AGCTCACCCC ACCAATGTAA GTCACCCAGT AACTGTTCTG 
CCAATGAACT CCTCCACTGT CGCTTTCTTT AGCACACCAC CAAAATTGCT CCAGAAAAGG 
GACGTGCAAT TGTCTGAAAC AGAGCCCAAT ATCTCAGACA TAGATCTCGT GTCAACAGCA 
TTGACCTTGA CCAGTGAACC CACACGGAGG CCCCAGACAG AGGCAATTAC CCACCCTGTT 
ACAGGCTTGA TTTTGAACAC AAGCACAGTG ACTCTGCCCC CCACAGGAAA TGTCACCAGG 
GAGACAACCA TTGCTGTGGT CACCACCACC AAATCTTTAC CATCTGATCA AGTCACCCTT 
GTGTATGACC AACAGGAAGT TGAGGCGATA TTCTTAATTA TCTTAGTGGT TGTCATAATA 
GGAGAATTTT TCAGTGCCTC TTCTGTCACG ATTGTAGATA CAGTAACCCT CCAGTACCTG 
GGAAAACACC GAGACCGATA TGGGCTACAG CGCATGTGGG GCTCCCTGGG CTGGGGCCTG 
GCCATGCTGT CTGTGGGTAT TGGAATCGAC TATACTCATA TAGATGTGCT CATTGATGGG 
AAGGGGTGTA AGCCCCCTGA GTACCGGAAC TACCAGATTG TCTTCATCGT CTTTGGAGTT 
CTCATGACCA TGGCCCTGAT CGTTGCCACT CAGTTCCGGT TCCGCTACAA CCACTTCAAC 
AATAGTGATG GTAAAGGGAA AGAGGTGGAG ATCCCACAGG TGGAAAGGGA CAACTCCACA 
GAGTCCTCTG AAGAGACTCC AACTGCCGCG ACTCACTCAC AGGCCTTCAA CTTCTGGGAC 
TTGATCAAGT TGCTATGCAG TGTGCAGTAT GGCTCGGTTC TGTTTGTGGC CTGGTTCATG 
GGTTTTGGAT ACGGCTTCGT GTTCACCTTT CTCTACTGGC ATCTGGAAGA CCTAAATGGA 
ACTACAACCC TCTTTGGGGT CTGTTCAGTC CTGAGTCATG TGTCCGAGTT GACCGCTTAC 
TTCTTCAGCC ACAAGCTTAT TGAGTTGATT GGCCATATCA GGGTTTTATA CATTGGCCTG 
GCCTGTAATA CAGCTCGCTA TATTTACATT TCCTACCTGG AAAATGCCTG GACTGTCCTC 
CCTATGGAAG TTCTTCAAGG TGTCACACAC GCAGCCATCT GGGCAGCGTG CATCTCTTAC 
CTCAGTGCAG CTGTCCCCCC TGAGCTGAGG ACTTCTGCAC AGGGAATCCT GCAAGGCCTT 
CATCTGGGTC TGGGGCGAGG ATGTGGTGCC ATGATCGGAG GCGTGTTAGT CAATTATTTT 
GGGGCTGCTG CAACTTTCCG AGGGATTGGC ATGGCTTGCC TGGTGATCCT TCTCCTCTTT 
GCCCTGATCC AGTGGCTGGC AGTACCAGAT GAAGAAGAAG ACAAGACAAT GTTGGCAGAA 
AGGATCCCAG TTCCCTCTAG TCCCGTTCCC ATAGCAACCA TTGACCTGGT ACAGCAACAG 
ACAGAAGATG TCATGCCACG CGTTGAGGCC AGACTTCCGC CCAAGAAAAC CAAGCACCAG 
GAAGAGCAGG AAGATGTAAA TAAACCGGCC TGGGGGGTCA GCTCCTCGCC TTGGGTGACC 
TTTGTCTATG CTCTCTACCA AGTTAAAGAG CTAATTCAAC TGACAAGAGA GAGCCGTGCT 
TCTGAGATAC AACCTTTACA GGGGACCAGT GAGAATCGGG AAGCCTCTCC AGCTGGTGGA 
GCCCAGCGTG CCCCACGTGA AACTCACTCC GCCTCACCTA GAAACCAGCC ATCCCCTGAC 
ACAGCAGCAT CTCAAACGCA GTCCAGCCCT GCTCACCCCA GTGCTACCCC GCATGGACAG 
GAGAGTGGAG AGAAGCAGGC TCAGCCCACT GTGGGAGAAC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Macrophage MHC and T-cell receptors essential for rejection of allografted skin and lymphoma.
Transplantation96(3)251-7(2013 Aug)
Tashiro-Yamaji J,Maeda S,Ikawa M,Okabe M,Kubota T,Yoshida R

A mutation in the Srrm4 gene causes alternative splicing defects and deafness in the Bronx waltzer mouse.
PLoS genetics8(10)e1002966(2012)
Nakano Y,Jahan I,Bonde G,Sun X,Hildebrand MS,Engelhardt JF,Smith RJ,Cornell RA,Fritzsch B,B?nfi B

HLA-B62 as a possible ligand for the human homologue of mouse macrophage MHC receptor 2 (MMR2) on monocytes.
Gene454(1-2)31-8(2010 Apr)
Shimizu T,Tashiro-Yamaji J,Hayashi M,Inoue Y,Ibata M,Kubota T,Tanigawa N,Yoshida R

Macrophage MHC receptor 2: a novel receptor on allograft (H-2D(d)K(d))-induced macrophage (H-2D(b)K(b)) recognizing an MHC class I molecule, H-2K(d), in mice.
Gene3841-8(2006 Dec)
Tashiro-Yamaji J,Kubota T,Yoshida R

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Mfsd6 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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