tra2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	tra2
Entrez Gene ID	36619
Full Name	transformer 2
Synonyms	CG10128,Dmel\CG10128,TRA 2,TRA-2,TRA2,Tra-2,Tra2,tra-2
General protein information	Preferred Names transformer 2 Names CG10128 gene product from transcript CG10128-RA CG10128-PA CG10128-PB CG10128-PC CG10128-PD CG10128-PE CG10128-PF CG10128-PG Transformer-2 tra2-PA tra2-PB tra2-PC tra2-PD tra2-PE tra2-PF tra2-PG transformer2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 51B6-51B6\|2-70 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_206112.2	NP_995834.1	transformer 2, isoform G
NM_057417.3	NP_476765.1	transformer 2, isoform C
NM_057416.3	NP_476764.1	transformer 2, isoform A
NM_057418.2	NP_476766.1	transformer 2, isoform E
NM_057415.3	NP_476763.1	transformer 2, isoform B
NM_206113.2	NP_995835.1	transformer 2, isoform F
NM_134280.1	NP_599107.1	transformer 2, isoform D

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	tra2	NP_476766.1
Danio rerio (zebrafish)	tra2b	NP_957491.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Together, these results suggest a link between mRNA splicing, sex determination and lipid metabolism and may provide insight into the mechanisms underlying tissue-specific splicing and nutrient storage.
Title: The splicing factor transformer2 (tra2) functions in the Drosophila fat body to regulate lipid storage.

results indicate the Hfp/Puf68 protein is required for the negative regulation of splicing by Tra2
Title: Half pint/Puf68 is required for negative regulation of splicing by the SR splicing factor Transformer2.

Anastrepha Tra2 protein supplies tra-2 function in Drosophila
Title: Effect of the transformer-2 gene of Anastrepha on the somatic sexual development of Drosophila.

Rbp1 and Tra2 bind to the ISS independently through distinct sequences suggesting that functionally synergistic interactions of these SR factors can cause either splicing activation or repression.
Title: The doublesex splicing enhancer components Tra2 and Rbp1 also repress splicing through an intronic silencer.

Here we show that that widely used tra2 mutant alleles have residual activity and are not suitable for evaluating its effect on viability.
Title: Analysis of a null mutation in the Drosophila splicing regulator Tra2 suggests its function is restricted to sexual differentiation.

The following tra2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tra2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa09990	NM_057416.3 Latest version!	Drosophila melanogaster transformer 2 (tra2), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa09990	NM_206112.2 Latest version!	Drosophila melanogaster transformer 2 (tra2), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa09990	NM_206113.2 Latest version!	Drosophila melanogaster transformer 2 (tra2), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa09991	NM_057415.3 Latest version!	Drosophila melanogaster transformer 2 (tra2), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa09992	NM_057417.3 Latest version!	Drosophila melanogaster transformer 2 (tra2), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa09992	NM_134280.1 Latest version!	Drosophila melanogaster transformer 2 (tra2), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa09993	NM_057418.2 Latest version!	Drosophila melanogaster transformer 2 (tra2), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tra2 ( NM_206112.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995834.1 Drosophila melanogaster transformer 2 (tra2), transcript variant G, mRNA.

Price & Availability↑

CloneID	OFa09990
Clone ID Related Accession (Same CDS sequence)	NM_206112.2 , NM_057416.3 , NM_206113.2
Accession Version	NM_206112.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	transformer 2, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_206112.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCGGG AGCCACTCAG TAGCGGGCGT CTCCATTGCA GCGCCAGATA TAAACACAAG 
CGATCGGCGT CATCATCGTC AGCGGGGACA ACTTCATCCG GACACAAGGA CCGCAGGTCT 
GACTACGATT ACTGTGGCAG TCGCCGGCAC CAGCGGTCCT CCTCTCGCCG ACGCTCCCGT 
TCGCGTTCCT CCTCGGAGTC GCCGCCACCG GAGCCGCGTC ATCGCTCCGG ACGTTCATCA 
CGCGATCGCG AACGAATGCA CAAGTCTCGC GAACATCCAC AAGCAAGCCG CTGCATAGGA 
GTCTTTGGAC TGAACACAAA TACCTCGCAG CACAAGGTAC GCGAGCTGTT CAACAAGTAC 
GGACCTATCG AACGCATCCA GATGGTGATT GACGCACAAA CACAGCGTTC CCGGGGCTTT 
TGTTTCATTT ACTTTGAGAA ACTCAGCGAT GCCCGCGCGG CTAAGGACAG CTGCTCCGGA 
ATAGAAGTGG ATGGTCGCCG TATTCGCGTC GATTTCTCTA TAACCCAACG GGCTCATACC 
CCAACTCCGG GTGTGTATTT GGGTCGTCAG CCGCGTGGAA AAGCTCCACG CTCATTTTCA 
CCGCGTAGAG GACGCCGTGT GTATCACGAT CGCTCCGCTT CGCCCTATGA CAACTATCGT 
GATCGCTATG ATTACCGCAA CGATCGCTAC GACCGTAATC TCCGCAGGAG CCCTAGTCGC 
AACCGTTACA CTCGCAACAG GAGCTACAGC CGTTCACGCT CTCCGCAACT ACGTCGAACT 
TCATCGCGCT ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995834.1
CDS	181..975
Translation	MDREPLSSGRLHCSARYKHKRSASSSSAGTTSSGHKDRRSDYDYCGSRRHQRSSSRRRSRSRSSSESPPPEPRHRSGRSSRDRERMHKSREHPQASRCIGVFGLNTNTSQHKVRELFNKYGPIERIQMVIDAQTQRSRGFCFIYFEKLSDARAAKDSCSGIEVDGRRIRVDFSITQRAHTPTPGVYLGRQPRGKAPRSFSPRRGRRVYHDRSASPYDNYRDRYDYRNDRYDRNLRRSPSRNRYTRNRSYSRSRSPQLRRTSSRY

Target ORF information:

RefSeq Version	NM_206112.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster transformer 2 (tra2), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206112.2

ORF Insert Sequence:

ATGGATCGGG AGCCACTCAG TAGCGGGCGT CTCCATTGCA GCGCCAGATA TAAACACAAG 
CGATCGGCGT CATCATCGTC AGCGGGGACA ACTTCATCCG GACACAAGGA CCGCAGGTCT 
GACTACGATT ACTGTGGCAG TCGCCGGCAC CAGCGGTCCT CCTCTCGCCG ACGCTCCCGT 
TCGCGTTCCT CCTCGGAGTC GCCGCCACCG GAGCCGCGTC ATCGCTCCGG ACGTTCATCA 
CGCGATCGCG AACGAATGCA CAAGTCTCGC GAACATCCAC AAGCAAGCCG CTGCATAGGA 
GTCTTTGGAC TGAACACAAA TACCTCGCAG CACAAGGTAC GCGAGCTGTT CAACAAGTAC 
GGACCTATCG AACGCATCCA GATGGTGATT GACGCACAAA CACAGCGTTC CCGGGGCTTT 
TGTTTCATTT ACTTTGAGAA ACTCAGCGAT GCCCGCGCGG CTAAGGACAG CTGCTCCGGA 
ATAGAAGTGG ATGGTCGCCG TATTCGCGTC GATTTCTCTA TAACCCAACG GGCTCATACC 
CCAACTCCGG GTGTGTATTT GGGTCGTCAG CCGCGTGGAA AAGCTCCACG CTCATTTTCA 
CCGCGTAGAG GACGCCGTGT GTATCACGAT CGCTCCGCTT CGCCCTATGA CAACTATCGT 
GATCGCTATG ATTACCGCAA CGATCGCTAC GACCGTAATC TCCGCAGGAG CCCTAGTCGC 
AACCGTTACA CTCGCAACAG GAGCTACAGC CGTTCACGCT CTCCGCAACT ACGTCGAACT 
TCATCGCGCT ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tra2 ( NM_057416.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476764.1 Drosophila melanogaster transformer 2 (tra2), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa09990
Clone ID Related Accession (Same CDS sequence)	NM_206112.2 , NM_057416.3 , NM_206113.2
Accession Version	NM_057416.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	transformer 2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_057416.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCGGG AGCCACTCAG TAGCGGGCGT CTCCATTGCA GCGCCAGATA TAAACACAAG 
CGATCGGCGT CATCATCGTC AGCGGGGACA ACTTCATCCG GACACAAGGA CCGCAGGTCT 
GACTACGATT ACTGTGGCAG TCGCCGGCAC CAGCGGTCCT CCTCTCGCCG ACGCTCCCGT 
TCGCGTTCCT CCTCGGAGTC GCCGCCACCG GAGCCGCGTC ATCGCTCCGG ACGTTCATCA 
CGCGATCGCG AACGAATGCA CAAGTCTCGC GAACATCCAC AAGCAAGCCG CTGCATAGGA 
GTCTTTGGAC TGAACACAAA TACCTCGCAG CACAAGGTAC GCGAGCTGTT CAACAAGTAC 
GGACCTATCG AACGCATCCA GATGGTGATT GACGCACAAA CACAGCGTTC CCGGGGCTTT 
TGTTTCATTT ACTTTGAGAA ACTCAGCGAT GCCCGCGCGG CTAAGGACAG CTGCTCCGGA 
ATAGAAGTGG ATGGTCGCCG TATTCGCGTC GATTTCTCTA TAACCCAACG GGCTCATACC 
CCAACTCCGG GTGTGTATTT GGGTCGTCAG CCGCGTGGAA AAGCTCCACG CTCATTTTCA 
CCGCGTAGAG GACGCCGTGT GTATCACGAT CGCTCCGCTT CGCCCTATGA CAACTATCGT 
GATCGCTATG ATTACCGCAA CGATCGCTAC GACCGTAATC TCCGCAGGAG CCCTAGTCGC 
AACCGTTACA CTCGCAACAG GAGCTACAGC CGTTCACGCT CTCCGCAACT ACGTCGAACT 
TCATCGCGCT ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476764.1
CDS	166..960
Translation	MDREPLSSGRLHCSARYKHKRSASSSSAGTTSSGHKDRRSDYDYCGSRRHQRSSSRRRSRSRSSSESPPPEPRHRSGRSSRDRERMHKSREHPQASRCIGVFGLNTNTSQHKVRELFNKYGPIERIQMVIDAQTQRSRGFCFIYFEKLSDARAAKDSCSGIEVDGRRIRVDFSITQRAHTPTPGVYLGRQPRGKAPRSFSPRRGRRVYHDRSASPYDNYRDRYDYRNDRYDRNLRRSPSRNRYTRNRSYSRSRSPQLRRTSSRY

Target ORF information:

RefSeq Version	NM_057416.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster transformer 2 (tra2), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057416.3

ORF Insert Sequence:

ATGGATCGGG AGCCACTCAG TAGCGGGCGT CTCCATTGCA GCGCCAGATA TAAACACAAG 
CGATCGGCGT CATCATCGTC AGCGGGGACA ACTTCATCCG GACACAAGGA CCGCAGGTCT 
GACTACGATT ACTGTGGCAG TCGCCGGCAC CAGCGGTCCT CCTCTCGCCG ACGCTCCCGT 
TCGCGTTCCT CCTCGGAGTC GCCGCCACCG GAGCCGCGTC ATCGCTCCGG ACGTTCATCA 
CGCGATCGCG AACGAATGCA CAAGTCTCGC GAACATCCAC AAGCAAGCCG CTGCATAGGA 
GTCTTTGGAC TGAACACAAA TACCTCGCAG CACAAGGTAC GCGAGCTGTT CAACAAGTAC 
GGACCTATCG AACGCATCCA GATGGTGATT GACGCACAAA CACAGCGTTC CCGGGGCTTT 
TGTTTCATTT ACTTTGAGAA ACTCAGCGAT GCCCGCGCGG CTAAGGACAG CTGCTCCGGA 
ATAGAAGTGG ATGGTCGCCG TATTCGCGTC GATTTCTCTA TAACCCAACG GGCTCATACC 
CCAACTCCGG GTGTGTATTT GGGTCGTCAG CCGCGTGGAA AAGCTCCACG CTCATTTTCA 
CCGCGTAGAG GACGCCGTGT GTATCACGAT CGCTCCGCTT CGCCCTATGA CAACTATCGT 
GATCGCTATG ATTACCGCAA CGATCGCTAC GACCGTAATC TCCGCAGGAG CCCTAGTCGC 
AACCGTTACA CTCGCAACAG GAGCTACAGC CGTTCACGCT CTCCGCAACT ACGTCGAACT 
TCATCGCGCT ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tra2 ( NM_206113.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995835.1 Drosophila melanogaster transformer 2 (tra2), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa09990
Clone ID Related Accession (Same CDS sequence)	NM_206112.2 , NM_057416.3 , NM_206113.2
Accession Version	NM_206113.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	transformer 2, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_206113.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCGGG AGCCACTCAG TAGCGGGCGT CTCCATTGCA GCGCCAGATA TAAACACAAG 
CGATCGGCGT CATCATCGTC AGCGGGGACA ACTTCATCCG GACACAAGGA CCGCAGGTCT 
GACTACGATT ACTGTGGCAG TCGCCGGCAC CAGCGGTCCT CCTCTCGCCG ACGCTCCCGT 
TCGCGTTCCT CCTCGGAGTC GCCGCCACCG GAGCCGCGTC ATCGCTCCGG ACGTTCATCA 
CGCGATCGCG AACGAATGCA CAAGTCTCGC GAACATCCAC AAGCAAGCCG CTGCATAGGA 
GTCTTTGGAC TGAACACAAA TACCTCGCAG CACAAGGTAC GCGAGCTGTT CAACAAGTAC 
GGACCTATCG AACGCATCCA GATGGTGATT GACGCACAAA CACAGCGTTC CCGGGGCTTT 
TGTTTCATTT ACTTTGAGAA ACTCAGCGAT GCCCGCGCGG CTAAGGACAG CTGCTCCGGA 
ATAGAAGTGG ATGGTCGCCG TATTCGCGTC GATTTCTCTA TAACCCAACG GGCTCATACC 
CCAACTCCGG GTGTGTATTT GGGTCGTCAG CCGCGTGGAA AAGCTCCACG CTCATTTTCA 
CCGCGTAGAG GACGCCGTGT GTATCACGAT CGCTCCGCTT CGCCCTATGA CAACTATCGT 
GATCGCTATG ATTACCGCAA CGATCGCTAC GACCGTAATC TCCGCAGGAG CCCTAGTCGC 
AACCGTTACA CTCGCAACAG GAGCTACAGC CGTTCACGCT CTCCGCAACT ACGTCGAACT 
TCATCGCGCT ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995835.1
CDS	171..965
Translation	MDREPLSSGRLHCSARYKHKRSASSSSAGTTSSGHKDRRSDYDYCGSRRHQRSSSRRRSRSRSSSESPPPEPRHRSGRSSRDRERMHKSREHPQASRCIGVFGLNTNTSQHKVRELFNKYGPIERIQMVIDAQTQRSRGFCFIYFEKLSDARAAKDSCSGIEVDGRRIRVDFSITQRAHTPTPGVYLGRQPRGKAPRSFSPRRGRRVYHDRSASPYDNYRDRYDYRNDRYDRNLRRSPSRNRYTRNRSYSRSRSPQLRRTSSRY

Target ORF information:

RefSeq Version	NM_206113.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster transformer 2 (tra2), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206113.2

ORF Insert Sequence:

ATGGATCGGG AGCCACTCAG TAGCGGGCGT CTCCATTGCA GCGCCAGATA TAAACACAAG 
CGATCGGCGT CATCATCGTC AGCGGGGACA ACTTCATCCG GACACAAGGA CCGCAGGTCT 
GACTACGATT ACTGTGGCAG TCGCCGGCAC CAGCGGTCCT CCTCTCGCCG ACGCTCCCGT 
TCGCGTTCCT CCTCGGAGTC GCCGCCACCG GAGCCGCGTC ATCGCTCCGG ACGTTCATCA 
CGCGATCGCG AACGAATGCA CAAGTCTCGC GAACATCCAC AAGCAAGCCG CTGCATAGGA 
GTCTTTGGAC TGAACACAAA TACCTCGCAG CACAAGGTAC GCGAGCTGTT CAACAAGTAC 
GGACCTATCG AACGCATCCA GATGGTGATT GACGCACAAA CACAGCGTTC CCGGGGCTTT 
TGTTTCATTT ACTTTGAGAA ACTCAGCGAT GCCCGCGCGG CTAAGGACAG CTGCTCCGGA 
ATAGAAGTGG ATGGTCGCCG TATTCGCGTC GATTTCTCTA TAACCCAACG GGCTCATACC 
CCAACTCCGG GTGTGTATTT GGGTCGTCAG CCGCGTGGAA AAGCTCCACG CTCATTTTCA 
CCGCGTAGAG GACGCCGTGT GTATCACGAT CGCTCCGCTT CGCCCTATGA CAACTATCGT 
GATCGCTATG ATTACCGCAA CGATCGCTAC GACCGTAATC TCCGCAGGAG CCCTAGTCGC 
AACCGTTACA CTCGCAACAG GAGCTACAGC CGTTCACGCT CTCCGCAACT ACGTCGAACT 
TCATCGCGCT ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tra2 ( NM_057415.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476763.1 Drosophila melanogaster transformer 2 (tra2), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa09991
Clone ID Related Accession (Same CDS sequence)	NM_057415.3
Accession Version	NM_057415.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	transformer 2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_057415.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCGGG AGCCACTCAG TAGCGGGCGT CTCCATTGCA GCGCCAGATA TAAACACAAG 
CGATCGGCGT CATCATCGTC AGCGGGGACA ACTTCATCCG GACACAAGGA CCGCAGGTCT 
GACTACGATT ACTGTGGCAG TCGCCGGCAC CAGCGGTCCT CCTCTCGCCG ACGCTCCCGT 
TCGCGTTCCT CCTCGGAGTC GCCGCCACCG GAGCCGCGTC ATCGCTCCGG ACGTTCATCA 
CGCGATCGCG AACGAATGCA CAAGTCTCGC GAACATCCAC AAGCAAGCCG CTGCATAGGA 
GTCTTTGGAC TGAACACAAA TACCTCGCAG CACAAGGTAC GCGAGCTGTT CAACAAGTAC 
GGACCTATCG AACGCATCCA GATGGTGATT GACGCACAAA TTAACCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476763.1
CDS	166..576
Translation	MDREPLSSGRLHCSARYKHKRSASSSSAGTTSSGHKDRRSDYDYCGSRRHQRSSSRRRSRSRSSSESPPPEPRHRSGRSSRDRERMHKSREHPQASRCIGVFGLNTNTSQHKVRELFNKYGPIERIQMVIDAQINP

Target ORF information:

RefSeq Version	NM_057415.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster transformer 2 (tra2), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057415.3

ORF Insert Sequence:

ATGGATCGGG AGCCACTCAG TAGCGGGCGT CTCCATTGCA GCGCCAGATA TAAACACAAG 
CGATCGGCGT CATCATCGTC AGCGGGGACA ACTTCATCCG GACACAAGGA CCGCAGGTCT 
GACTACGATT ACTGTGGCAG TCGCCGGCAC CAGCGGTCCT CCTCTCGCCG ACGCTCCCGT 
TCGCGTTCCT CCTCGGAGTC GCCGCCACCG GAGCCGCGTC ATCGCTCCGG ACGTTCATCA 
CGCGATCGCG AACGAATGCA CAAGTCTCGC GAACATCCAC AAGCAAGCCG CTGCATAGGA 
GTCTTTGGAC TGAACACAAA TACCTCGCAG CACAAGGTAC GCGAGCTGTT CAACAAGTAC 
GGACCTATCG AACGCATCCA GATGGTGATT GACGCACAAA TTAACCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tra2 ( NM_057417.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476765.1 Drosophila melanogaster transformer 2 (tra2), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa09992
Clone ID Related Accession (Same CDS sequence)	NM_057417.3 , NM_134280.1
Accession Version	NM_057417.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	transformer 2, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_057417.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGACT ACGATTACTG TGGCAGTCGC CGGCACCAGC GGTCCTCCTC TCGCCGACGC 
TCCCGTTCGC GTTCCTCCTC GGAGTCGCCG CCACCGGAGC CGCGTCATCG CTCCGGACGT 
TCATCACGCG ATCGCGAACG AATGCACAAG TCTCGCGAAC ATCCACAAGC AAGCCGCTGC 
ATAGGAGTCT TTGGACTGAA CACAAATACC TCGCAGCACA AGGTACGCGA GCTGTTCAAC 
AAGTACGGAC CTATCGAACG CATCCAGATG GTGATTGACG CACAAACACA GCGTTCCCGG 
GGCTTTTGTT TCATTTACTT TGAGAAACTC AGCGATGCCC GCGCGGCTAA GGACAGCTGC 
TCCGGAATAG AAGTGGATGG TCGCCGTATT CGCGTCGATT TCTCTATAAC CCAACGGGCT 
CATACCCCAA CTCCGGGTGT GTATTTGGGT CGTCAGCCGC GTGGAAAAGC TCCACGCTCA 
TTTTCACCGC GTAGAGGACG CCGTGTGTAT CACGATCGCT CCGCTTCGCC CTATGACAAC 
TATCGTGATC GCTATGATTA CCGCAACGAT CGCTACGACC GTAATCTCCG CAGGAGCCCT 
AGTCGCAACC GTTACACTCG CAACAGGAGC TACAGCCGTT CACGCTCTCC GCAACTACGT 
CGAACTTCAT CGCGCTATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476765.1
CDS	472..1152
Translation	MSDYDYCGSRRHQRSSSRRRSRSRSSSESPPPEPRHRSGRSSRDRERMHKSREHPQASRCIGVFGLNTNTSQHKVRELFNKYGPIERIQMVIDAQTQRSRGFCFIYFEKLSDARAAKDSCSGIEVDGRRIRVDFSITQRAHTPTPGVYLGRQPRGKAPRSFSPRRGRRVYHDRSASPYDNYRDRYDYRNDRYDRNLRRSPSRNRYTRNRSYSRSRSPQLRRTSSRY

Target ORF information:

RefSeq Version	NM_057417.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster transformer 2 (tra2), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057417.3

ORF Insert Sequence:

ATGTCTGACT ACGATTACTG TGGCAGTCGC CGGCACCAGC GGTCCTCCTC TCGCCGACGC 
TCCCGTTCGC GTTCCTCCTC GGAGTCGCCG CCACCGGAGC CGCGTCATCG CTCCGGACGT 
TCATCACGCG ATCGCGAACG AATGCACAAG TCTCGCGAAC ATCCACAAGC AAGCCGCTGC 
ATAGGAGTCT TTGGACTGAA CACAAATACC TCGCAGCACA AGGTACGCGA GCTGTTCAAC 
AAGTACGGAC CTATCGAACG CATCCAGATG GTGATTGACG CACAAACACA GCGTTCCCGG 
GGCTTTTGTT TCATTTACTT TGAGAAACTC AGCGATGCCC GCGCGGCTAA GGACAGCTGC 
TCCGGAATAG AAGTGGATGG TCGCCGTATT CGCGTCGATT TCTCTATAAC CCAACGGGCT 
CATACCCCAA CTCCGGGTGT GTATTTGGGT CGTCAGCCGC GTGGAAAAGC TCCACGCTCA 
TTTTCACCGC GTAGAGGACG CCGTGTGTAT CACGATCGCT CCGCTTCGCC CTATGACAAC 
TATCGTGATC GCTATGATTA CCGCAACGAT CGCTACGACC GTAATCTCCG CAGGAGCCCT 
AGTCGCAACC GTTACACTCG CAACAGGAGC TACAGCCGTT CACGCTCTCC GCAACTACGT 
CGAACTTCAT CGCGCTATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tra2 ( NM_134280.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_599107.1 Drosophila melanogaster transformer 2 (tra2), transcript variant D, mRNA.

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CloneID	OFa09992
Clone ID Related Accession (Same CDS sequence)	NM_057417.3 , NM_134280.1
Accession Version	NM_134280.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	transformer 2, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGACT ACGATTACTG TGGCAGTCGC CGGCACCAGC GGTCCTCCTC TCGCCGACGC 
TCCCGTTCGC GTTCCTCCTC GGAGTCGCCG CCACCGGAGC CGCGTCATCG CTCCGGACGT 
TCATCACGCG ATCGCGAACG AATGCACAAG TCTCGCGAAC ATCCACAAGC AAGCCGCTGC 
ATAGGAGTCT TTGGACTGAA CACAAATACC TCGCAGCACA AGGTACGCGA GCTGTTCAAC 
AAGTACGGAC CTATCGAACG CATCCAGATG GTGATTGACG CACAAACACA GCGTTCCCGG 
GGCTTTTGTT TCATTTACTT TGAGAAACTC AGCGATGCCC GCGCGGCTAA GGACAGCTGC 
TCCGGAATAG AAGTGGATGG TCGCCGTATT CGCGTCGATT TCTCTATAAC CCAACGGGCT 
CATACCCCAA CTCCGGGTGT GTATTTGGGT CGTCAGCCGC GTGGAAAAGC TCCACGCTCA 
TTTTCACCGC GTAGAGGACG CCGTGTGTAT CACGATCGCT CCGCTTCGCC CTATGACAAC 
TATCGTGATC GCTATGATTA CCGCAACGAT CGCTACGACC GTAATCTCCG CAGGAGCCCT 
AGTCGCAACC GTTACACTCG CAACAGGAGC TACAGCCGTT CACGCTCTCC GCAACTACGT 
CGAACTTCAT CGCGCTATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_599107.1
CDS	132..812
Translation	MSDYDYCGSRRHQRSSSRRRSRSRSSSESPPPEPRHRSGRSSRDRERMHKSREHPQASRCIGVFGLNTNTSQHKVRELFNKYGPIERIQMVIDAQTQRSRGFCFIYFEKLSDARAAKDSCSGIEVDGRRIRVDFSITQRAHTPTPGVYLGRQPRGKAPRSFSPRRGRRVYHDRSASPYDNYRDRYDYRNDRYDRNLRRSPSRNRYTRNRSYSRSRSPQLRRTSSRY

Target ORF information:

RefSeq Version	NM_134280.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster transformer 2 (tra2), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134280.1

ORF Insert Sequence:

ATGTCTGACT ACGATTACTG TGGCAGTCGC CGGCACCAGC GGTCCTCCTC TCGCCGACGC 
TCCCGTTCGC GTTCCTCCTC GGAGTCGCCG CCACCGGAGC CGCGTCATCG CTCCGGACGT 
TCATCACGCG ATCGCGAACG AATGCACAAG TCTCGCGAAC ATCCACAAGC AAGCCGCTGC 
ATAGGAGTCT TTGGACTGAA CACAAATACC TCGCAGCACA AGGTACGCGA GCTGTTCAAC 
AAGTACGGAC CTATCGAACG CATCCAGATG GTGATTGACG CACAAACACA GCGTTCCCGG 
GGCTTTTGTT TCATTTACTT TGAGAAACTC AGCGATGCCC GCGCGGCTAA GGACAGCTGC 
TCCGGAATAG AAGTGGATGG TCGCCGTATT CGCGTCGATT TCTCTATAAC CCAACGGGCT 
CATACCCCAA CTCCGGGTGT GTATTTGGGT CGTCAGCCGC GTGGAAAAGC TCCACGCTCA 
TTTTCACCGC GTAGAGGACG CCGTGTGTAT CACGATCGCT CCGCTTCGCC CTATGACAAC 
TATCGTGATC GCTATGATTA CCGCAACGAT CGCTACGACC GTAATCTCCG CAGGAGCCCT 
AGTCGCAACC GTTACACTCG CAACAGGAGC TACAGCCGTT CACGCTCTCC GCAACTACGT 
CGAACTTCAT CGCGCTATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tra2 ( NM_057418.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476766.1 Drosophila melanogaster transformer 2 (tra2), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa09993
Clone ID Related Accession (Same CDS sequence)	NM_057418.2
Accession Version	NM_057418.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	transformer 2, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Mar 19, 2002 this sequence version replaced NM_057418.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCACAAGT CTCGCGAACA TCCACAAGCA AGCCGCTGCA TAGGAGTCTT TGGACTGAAC 
ACAAATACCT CGCAGCACAA GGTACGCGAG CTGTTCAACA AGTACGGACC TATCGAACGC 
ATCCAGATGG TGATTGACGC ACAAACACAG CGTTCCCGGG GCTTTTGTTT CATTTACTTT 
GAGAAACTCA GCGATGCCCG CGCGGCTAAG GACAGCTGCT CCGGAATAGA AGTGGATGGT 
CGCCGTATTC GCGTCGATTT CTCTATAACC CAACGGGCTC ATACCCCAAC TCCGGGTGTG 
TATTTGGGTC GTCAGCCGCG TGGAAAAGCT CCACGCTCAT TTTCACCGCG TAGAGGACGC 
CGTGTGTATC ACGATCGCTC CGCTTCGCCC TATGACAACT ATCGTGATCG CTATGATTAC 
CGCAACGATC GCTACGACCG TAATCTCCGC AGGAGCCCTA GTCGCAACCG TTACACTCGC 
AACAGGAGCT ACAGCCGTTC ACGCTCTCCG CAACTACGTC GAACTTCATC GCGCTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476766.1
CDS	505..1044
Translation	MHKSREHPQASRCIGVFGLNTNTSQHKVRELFNKYGPIERIQMVIDAQTQRSRGFCFIYFEKLSDARAAKDSCSGIEVDGRRIRVDFSITQRAHTPTPGVYLGRQPRGKAPRSFSPRRGRRVYHDRSASPYDNYRDRYDYRNDRYDRNLRRSPSRNRYTRNRSYSRSRSPQLRRTSSRY

Target ORF information:

RefSeq Version	NM_057418.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster transformer 2 (tra2), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057418.2

ORF Insert Sequence:

ATGCACAAGT CTCGCGAACA TCCACAAGCA AGCCGCTGCA TAGGAGTCTT TGGACTGAAC 
ACAAATACCT CGCAGCACAA GGTACGCGAG CTGTTCAACA AGTACGGACC TATCGAACGC 
ATCCAGATGG TGATTGACGC ACAAACACAG CGTTCCCGGG GCTTTTGTTT CATTTACTTT 
GAGAAACTCA GCGATGCCCG CGCGGCTAAG GACAGCTGCT CCGGAATAGA AGTGGATGGT 
CGCCGTATTC GCGTCGATTT CTCTATAACC CAACGGGCTC ATACCCCAAC TCCGGGTGTG 
TATTTGGGTC GTCAGCCGCG TGGAAAAGCT CCACGCTCAT TTTCACCGCG TAGAGGACGC 
CGTGTGTATC ACGATCGCTC CGCTTCGCCC TATGACAACT ATCGTGATCG CTATGATTAC 
CGCAACGATC GCTACGACCG TAATCTCCGC AGGAGCCCTA GTCGCAACCG TTACACTCGC 
AACAGGAGCT ACAGCCGTTC ACGCTCTCCG CAACTACGTC GAACTTCATC GCGCTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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tra2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

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Target ORF information:

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Target ORF information:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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