bbc cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	bbc
Entrez Gene ID	36496
Full Name	bb in a boxcar
Synonyms	Bbc,CG6016,Dmel\CG6016,cept,dCCS2
General protein information	Preferred Names bb in a boxcar Names CG6016 gene product from transcript CG6016-RC CDP-ethanolamine phosphotransferase CG6016-PA CG6016-PB CG6016-PC CG6016-PE bbc-PA bbc-PB bbc-PC bbc-PE
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 50A11-50A13

mRNA and Protein(s)

mRNA	Protein	Name
NM_137033.4	NP_610877.2	bb in a boxcar, isoform A
NM_165993.3	NP_725301.1	bb in a boxcar, isoform B
NM_001259371.2	NP_001246300.1	bb in a boxcar, isoform C
NM_001274026.1	NP_001260955.1	bb in a boxcar, isoform E

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	bbc	NP_610877.2
Danio rerio (zebrafish)	cept1a	NP_001138264.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011766	XP_552478.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following bbc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bbc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa09875	NM_165993.3 Latest version!	Drosophila melanogaster bb in a boxcar (bbc), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa09876	NM_137033.4 Latest version!	Drosophila melanogaster bb in a boxcar (bbc), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa13006	NM_001259371.2 Latest version!	Drosophila melanogaster bb in a boxcar (bbc), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa13007	NM_001274026.1 Latest version!	Drosophila melanogaster bb in a boxcar (bbc), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bbc ( NM_165993.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725301.1 Drosophila melanogaster bb in a boxcar (bbc), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa09875
Clone ID Related Accession (Same CDS sequence)	NM_165993.3
Accession Version	NM_165993.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bb in a boxcar, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165993.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGCTGC TCGCCTACCG GGACAAGCAC ATCCTGAGTG CCCAGCAGCT GAGGAAGCTC 
AGCGAACACA AGTACTCCTG CTTCAGTGTC AGCCTGCTCG ATCCGCTGCT GCAGCCGTGG 
TGGAACTGGC TGGTCGCCCA GACGCCCCTT TGGCTGGCCC CCAATCTCAT TACCATCGTG 
GGACTGATCC TCAATGTGGT CACCACCCTG ATATTAATCT GTTATAGCCC AAATGGCGTT 
GAGGCTCCGC CGCGTTGGAC CTGCTTGCTC TGCGCGCTGG GTTTGTTCAT CTACCAGAGT 
CTGGACTCCA TCGACGGGAA GCAGGCGCGA CGCACGAATA CCTCATCGCC GTTGGGCGAA 
CTTTTCGACC ATGGCTGTGA CTCCATATCG ACGGTGTTTG TGGCCCTCTC GGCGTGCATC 
TCCTGCCAAC TGGGCCACTA TCCCAACTGG CTGTTCTTCC AGTGCTTCTG TGCTATTGCG 
CTCTTCTACT GCGCCCACTG GCAGACGTAT GTGTCTGGAA CGATGCGGTT CGGCAGAATT 
GATGTGACAG AGGCTCAGTT CTCAATTATA GCCATTCATT TGGTTTCGGC TGCACTGGGT 
CCTGAGATCT GGCTCACCAA GATCGGCATT GGCAGTATAC AGCTTTGGTA TGGGCCGGCA 
GTGACGACAA TTGTGTGCGG TCTGCTCTCC CTAACTTATG TGTTTTCCGT CATTAAAGCC 
GGTGGTGTTG GCAAGAACGG CTCGACAGTT GCTGGCACCA GTGTCCTCTC GCCCAGCATT 
CCGCTCACGC TGGTGGTGCT GCCCGCCCTG ATGATTGCCC AGAAGTCGCC GCAGAACCTC 
TTCACCGAGC ACGCATCGGT GTACATCATG GCGTTCGGCA TGGTGGCCGC CAAGGTCACC 
AATAAGCTGG TGATCGCTCA CATGACCAAG GCGGAGATGG AGTATCTGGA CTGGTCGCTG 
CTCGGCCCCT CGCTGCTGTT CCTCAACCAG TACTTCAACT GCATCGTGCC AGAGATCTGG 
CTACTGTGGT TCACGCTCAT CTGGGGCACC CAGGATCTGC TGCGCTACTG CGCCCAGGTG 
TGCCTCGAGA TTTGCCAGCA CCTGCGCATC GATCTCTTCC GGATACCGTA CACGCCCAAA 
GGCGCGACCC CGCATCCAGC CACCGCATCG GTGAGCAGCC AGAGCAACTT TGGCAGCAGT 
GCGGACAAGA ACGGTGGCAG CGCGCATCGA AAGTCGAAGA GCAAACCGCA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725301.1
CDS	392..1645
Translation	MALLAYRDKHILSAQQLRKLSEHKYSCFSVSLLDPLLQPWWNWLVAQTPLWLAPNLITIVGLILNVVTTLILICYSPNGVEAPPRWTCLLCALGLFIYQSLDSIDGKQARRTNTSSPLGELFDHGCDSISTVFVALSACISCQLGHYPNWLFFQCFCAIALFYCAHWQTYVSGTMRFGRIDVTEAQFSIIAIHLVSAALGPEIWLTKIGIGSIQLWYGPAVTTIVCGLLSLTYVFSVIKAGGVGKNGSTVAGTSVLSPSIPLTLVVLPALMIAQKSPQNLFTEHASVYIMAFGMVAAKVTNKLVIAHMTKAEMEYLDWSLLGPSLLFLNQYFNCIVPEIWLLWFTLIWGTQDLLRYCAQVCLEICQHLRIDLFRIPYTPKGATPHPATASVSSQSNFGSSADKNGGSAHRKSKSKPH

Target ORF information:

RefSeq Version	NM_165993.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bb in a boxcar (bbc), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165993.3

ORF Insert Sequence:

ATGGCGCTGC TCGCCTACCG GGACAAGCAC ATCCTGAGTG CCCAGCAGCT GAGGAAGCTC 
AGCGAACACA AGTACTCCTG CTTCAGTGTC AGCCTGCTCG ATCCGCTGCT GCAGCCGTGG 
TGGAACTGGC TGGTCGCCCA GACGCCCCTT TGGCTGGCCC CCAATCTCAT TACCATCGTG 
GGACTGATCC TCAATGTGGT CACCACCCTG ATATTAATCT GTTATAGCCC AAATGGCGTT 
GAGGCTCCGC CGCGTTGGAC CTGCTTGCTC TGCGCGCTGG GTTTGTTCAT CTACCAGAGT 
CTGGACTCCA TCGACGGGAA GCAGGCGCGA CGCACGAATA CCTCATCGCC GTTGGGCGAA 
CTTTTCGACC ATGGCTGTGA CTCCATATCG ACGGTGTTTG TGGCCCTCTC GGCGTGCATC 
TCCTGCCAAC TGGGCCACTA TCCCAACTGG CTGTTCTTCC AGTGCTTCTG TGCTATTGCG 
CTCTTCTACT GCGCCCACTG GCAGACGTAT GTGTCTGGAA CGATGCGGTT CGGCAGAATT 
GATGTGACAG AGGCTCAGTT CTCAATTATA GCCATTCATT TGGTTTCGGC TGCACTGGGT 
CCTGAGATCT GGCTCACCAA GATCGGCATT GGCAGTATAC AGCTTTGGTA TGGGCCGGCA 
GTGACGACAA TTGTGTGCGG TCTGCTCTCC CTAACTTATG TGTTTTCCGT CATTAAAGCC 
GGTGGTGTTG GCAAGAACGG CTCGACAGTT GCTGGCACCA GTGTCCTCTC GCCCAGCATT 
CCGCTCACGC TGGTGGTGCT GCCCGCCCTG ATGATTGCCC AGAAGTCGCC GCAGAACCTC 
TTCACCGAGC ACGCATCGGT GTACATCATG GCGTTCGGCA TGGTGGCCGC CAAGGTCACC 
AATAAGCTGG TGATCGCTCA CATGACCAAG GCGGAGATGG AGTATCTGGA CTGGTCGCTG 
CTCGGCCCCT CGCTGCTGTT CCTCAACCAG TACTTCAACT GCATCGTGCC AGAGATCTGG 
CTACTGTGGT TCACGCTCAT CTGGGGCACC CAGGATCTGC TGCGCTACTG CGCCCAGGTG 
TGCCTCGAGA TTTGCCAGCA CCTGCGCATC GATCTCTTCC GGATACCGTA CACGCCCAAA 
GGCGCGACCC CGCATCCAGC CACCGCATCG GTGAGCAGCC AGAGCAACTT TGGCAGCAGT 
GCGGACAAGA ACGGTGGCAG CGCGCATCGA AAGTCGAAGA GCAAACCGCA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bbc ( NM_137033.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610877.2 Drosophila melanogaster bb in a boxcar (bbc), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa09876
Clone ID Related Accession (Same CDS sequence)	NM_137033.4
Accession Version	NM_137033.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bb in a boxcar, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_137033.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGCTGC TCGCCTACCG GGACAAGCAC ATCCTGAGTG CCCAGCAGCT GAGGAAGCTC 
AGCGAACACA AGTACTCCTG CTTCAGTGTC AGCCTGCTCG ATCCGCTGCT GCAGCCGTGG 
TGGAACTGGC TGGTCGCCCA GACGCCCCTT TGGCTGGCCC CCAATCTCAT TACCATCGTG 
GGACTGATCC TCAATGTGGT CACCACCCTG ATATTAATCT GTTATAGCCC AAATGGCGTT 
GAGGCTCCGC CGCGTTGGAC CTGCTTGCTC TGCGCGCTGG GTTTGTTCAT CTACCAGAGT 
CTGGACTCCA TCGACGGGAA GCAGGCGCGA CGCACGAATA CCTCATCGCC GTTGGGCGAA 
CTTTTCGACC ATGGCTGTGA CTCCATATCG ACGGTGTTTG TGGCCCTCTC GGCGTGCATC 
TCCTGCCAAC TGGGCCACTA TCCCAACTGG CTGTTCTTCC AGTGCTTCTG TGCTATTGCG 
CTCTTCTACT GCGCCCACTG GCAGACGTAT GTGTCTGGAA CGATGCGGTT CGGCAGAATT 
GATGTGACAG AGGCTCAGTT CTCAATTATA GCCATTCATT TGGTTTCGGC TGCACTGGGT 
CCTGAGATCT GGCTCACCAA GATACCAATC GTGGGCCTTT CATGGAATTA CACAATATTA 
GTATTTATCA CATTCGGTTA TACCTTGAAT ATAATCAATT TCCTAAAAAT GTTTACTCAA 
GGCGGCAGTG GCAAAAACGG CTCCTCAGTT GCTGGCACCA GTGTCCTCTC GCCCAGCATT 
CCGCTCACGC TGGTGGTGCT GCCCGCCCTG ATGATTGCCC AGAAGTCGCC GCAGAACCTC 
TTCACCGAGC ACGCATCGGT GTACATCATG GCGTTCGGCA TGGTGGCCGC CAAGGTCACC 
AATAAGCTGG TGATCGCTCA CATGACCAAG GCGGAGATGG AGTATCTGGA CTGGTCGCTG 
CTCGGCCCCT CGCTGCTGTT CCTCAACCAG TACTTCAACT GCATCGTGCC AGAGATCTGG 
CTACTGTGGT TCACGCTCAT CTGGGGCACC CAGGATCTGC TGCGCTACTG CGCCCAGGTG 
TGCCTCGAGA TTTGCCAGCA CCTGCGCATC GATCTCTTCC GGATACCGTA CACGCCCAAA 
GGCGCGACCC CGCATCCAGC CACCGCATCG GTGAGCAGCC AGAGCAACTT TGGCAGCAGT 
GCGGACAAGA ACGGTGGCAG CGCGCATCGA AAGTCGAAGA GCAAACCGCA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610877.2
CDS	392..1645
Translation	MALLAYRDKHILSAQQLRKLSEHKYSCFSVSLLDPLLQPWWNWLVAQTPLWLAPNLITIVGLILNVVTTLILICYSPNGVEAPPRWTCLLCALGLFIYQSLDSIDGKQARRTNTSSPLGELFDHGCDSISTVFVALSACISCQLGHYPNWLFFQCFCAIALFYCAHWQTYVSGTMRFGRIDVTEAQFSIIAIHLVSAALGPEIWLTKIPIVGLSWNYTILVFITFGYTLNIINFLKMFTQGGSGKNGSSVAGTSVLSPSIPLTLVVLPALMIAQKSPQNLFTEHASVYIMAFGMVAAKVTNKLVIAHMTKAEMEYLDWSLLGPSLLFLNQYFNCIVPEIWLLWFTLIWGTQDLLRYCAQVCLEICQHLRIDLFRIPYTPKGATPHPATASVSSQSNFGSSADKNGGSAHRKSKSKPH

Target ORF information:

RefSeq Version	NM_137033.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bb in a boxcar (bbc), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137033.4

ORF Insert Sequence:

ATGGCGCTGC TCGCCTACCG GGACAAGCAC ATCCTGAGTG CCCAGCAGCT GAGGAAGCTC 
AGCGAACACA AGTACTCCTG CTTCAGTGTC AGCCTGCTCG ATCCGCTGCT GCAGCCGTGG 
TGGAACTGGC TGGTCGCCCA GACGCCCCTT TGGCTGGCCC CCAATCTCAT TACCATCGTG 
GGACTGATCC TCAATGTGGT CACCACCCTG ATATTAATCT GTTATAGCCC AAATGGCGTT 
GAGGCTCCGC CGCGTTGGAC CTGCTTGCTC TGCGCGCTGG GTTTGTTCAT CTACCAGAGT 
CTGGACTCCA TCGACGGGAA GCAGGCGCGA CGCACGAATA CCTCATCGCC GTTGGGCGAA 
CTTTTCGACC ATGGCTGTGA CTCCATATCG ACGGTGTTTG TGGCCCTCTC GGCGTGCATC 
TCCTGCCAAC TGGGCCACTA TCCCAACTGG CTGTTCTTCC AGTGCTTCTG TGCTATTGCG 
CTCTTCTACT GCGCCCACTG GCAGACGTAT GTGTCTGGAA CGATGCGGTT CGGCAGAATT 
GATGTGACAG AGGCTCAGTT CTCAATTATA GCCATTCATT TGGTTTCGGC TGCACTGGGT 
CCTGAGATCT GGCTCACCAA GATACCAATC GTGGGCCTTT CATGGAATTA CACAATATTA 
GTATTTATCA CATTCGGTTA TACCTTGAAT ATAATCAATT TCCTAAAAAT GTTTACTCAA 
GGCGGCAGTG GCAAAAACGG CTCCTCAGTT GCTGGCACCA GTGTCCTCTC GCCCAGCATT 
CCGCTCACGC TGGTGGTGCT GCCCGCCCTG ATGATTGCCC AGAAGTCGCC GCAGAACCTC 
TTCACCGAGC ACGCATCGGT GTACATCATG GCGTTCGGCA TGGTGGCCGC CAAGGTCACC 
AATAAGCTGG TGATCGCTCA CATGACCAAG GCGGAGATGG AGTATCTGGA CTGGTCGCTG 
CTCGGCCCCT CGCTGCTGTT CCTCAACCAG TACTTCAACT GCATCGTGCC AGAGATCTGG 
CTACTGTGGT TCACGCTCAT CTGGGGCACC CAGGATCTGC TGCGCTACTG CGCCCAGGTG 
TGCCTCGAGA TTTGCCAGCA CCTGCGCATC GATCTCTTCC GGATACCGTA CACGCCCAAA 
GGCGCGACCC CGCATCCAGC CACCGCATCG GTGAGCAGCC AGAGCAACTT TGGCAGCAGT 
GCGGACAAGA ACGGTGGCAG CGCGCATCGA AAGTCGAAGA GCAAACCGCA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bbc ( NM_001259371.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246300.1 Drosophila melanogaster bb in a boxcar (bbc), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa13006
Clone ID Related Accession (Same CDS sequence)	NM_001259371.2
Accession Version	NM_001259371.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bb in a boxcar, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001259371.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGCTGC TCGCCTACCG GGACAAGCAC ATCCTGAGTG CCCAGCAGCT GAGGAAGCTC 
AGCGAACACA AGTACTCCTG CTTCAGTGTC AGCCTGCTCG ATCCGCTGCT GCAGCCGTGG 
TGGAACTGGC TGGTCGCCCA GACGCCCCTT TGGCTGGCCC CCAATCTCAT TACCATCGTG 
GGACTGATCC TCAATGTGGT CACCACCCTG ATATTAATCT GTTATAGCCC AAATGGCGTT 
GAGGCTCCGC CGCGTTGGAC CTGCTTGCTC TGCGCGCTGG GTTTGTTCAT CTACCAGAGT 
CTGGACTCCA TCGACGGGAA GCAGGCGCGA CGCACGAATA CCTCATCGCC GTTGGGCGAA 
CTTTTCGACC ATGGCTGTGA CTCCATATCG ACGGTGTTTG TGGCCCTCTC GGCGTGCATC 
TCCTGCCAAC TGGGCCACTA TCCCAACTGG CTGTTCTTCC AGTGCTTCTG TGCTATTGCG 
CTCTTCTACT GCGCCCACTG GCAGACGTAT GTGTCTGGAA CGATGCGGTT CGGCAGAATT 
GATGTGACAG AGGCTCAGTT CTCAATTATA GCCATTCATT TGGTTTCGGC TGCACTGGGT 
CCTGAGATCT GGCTCACCAA GCTGCCGCAC TTCAACTGCG AATCGCGATT TCTGCCAATT 
TATGTGGCCT GCGGCGTTGA TTTGATTCTA GCACTGCGCT ACACGAAATG CATTTTGACC 
GAAGGTTGCG GCAAGAACGG ATCATCGGTT GCTGGCACCA GTGTCCTCTC GCCCAGCATT 
CCGCTCACGC TGGTGGTGCT GCCCGCCCTG ATGATTGCCC AGAAGTCGCC GCAGAACCTC 
TTCACCGAGC ACGCATCGGT GTACATCATG GCGTTCGGCA TGGTGGCCGC CAAGGTCACC 
AATAAGCTGG TGATCGCTCA CATGACCAAG GCGGAGATGG AGTATCTGGA CTGGTCGCTG 
CTCGGCCCCT CGCTGCTGTT CCTCAACCAG TACTTCAACT GCATCGTGCC AGAGATCTGG 
CTACTGTGGT TCACGCTCAT CTGGGGCACC CAGGATCTGC TGCGCTACTG CGCCCAGGTG 
TGCCTCGAGA TTTGCCAGCA CCTGCGCATC GATCTCTTCC GGATACCGTA CACGCCCAAA 
GGCGCGACCC CGCATCCAGC CACCGCATCG GTGAGCAGCC AGAGCAACTT TGGCAGCAGT 
GCGGACAAGA ACGGTGGCAG CGCGCATCGA AAGTCGAAGA GCAAACCGCA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246300.1
CDS	392..1645
Translation	MALLAYRDKHILSAQQLRKLSEHKYSCFSVSLLDPLLQPWWNWLVAQTPLWLAPNLITIVGLILNVVTTLILICYSPNGVEAPPRWTCLLCALGLFIYQSLDSIDGKQARRTNTSSPLGELFDHGCDSISTVFVALSACISCQLGHYPNWLFFQCFCAIALFYCAHWQTYVSGTMRFGRIDVTEAQFSIIAIHLVSAALGPEIWLTKLPHFNCESRFLPIYVACGVDLILALRYTKCILTEGCGKNGSSVAGTSVLSPSIPLTLVVLPALMIAQKSPQNLFTEHASVYIMAFGMVAAKVTNKLVIAHMTKAEMEYLDWSLLGPSLLFLNQYFNCIVPEIWLLWFTLIWGTQDLLRYCAQVCLEICQHLRIDLFRIPYTPKGATPHPATASVSSQSNFGSSADKNGGSAHRKSKSKPH

Target ORF information:

RefSeq Version	NM_001259371.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bb in a boxcar (bbc), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259371.2

ORF Insert Sequence:

ATGGCGCTGC TCGCCTACCG GGACAAGCAC ATCCTGAGTG CCCAGCAGCT GAGGAAGCTC 
AGCGAACACA AGTACTCCTG CTTCAGTGTC AGCCTGCTCG ATCCGCTGCT GCAGCCGTGG 
TGGAACTGGC TGGTCGCCCA GACGCCCCTT TGGCTGGCCC CCAATCTCAT TACCATCGTG 
GGACTGATCC TCAATGTGGT CACCACCCTG ATATTAATCT GTTATAGCCC AAATGGCGTT 
GAGGCTCCGC CGCGTTGGAC CTGCTTGCTC TGCGCGCTGG GTTTGTTCAT CTACCAGAGT 
CTGGACTCCA TCGACGGGAA GCAGGCGCGA CGCACGAATA CCTCATCGCC GTTGGGCGAA 
CTTTTCGACC ATGGCTGTGA CTCCATATCG ACGGTGTTTG TGGCCCTCTC GGCGTGCATC 
TCCTGCCAAC TGGGCCACTA TCCCAACTGG CTGTTCTTCC AGTGCTTCTG TGCTATTGCG 
CTCTTCTACT GCGCCCACTG GCAGACGTAT GTGTCTGGAA CGATGCGGTT CGGCAGAATT 
GATGTGACAG AGGCTCAGTT CTCAATTATA GCCATTCATT TGGTTTCGGC TGCACTGGGT 
CCTGAGATCT GGCTCACCAA GCTGCCGCAC TTCAACTGCG AATCGCGATT TCTGCCAATT 
TATGTGGCCT GCGGCGTTGA TTTGATTCTA GCACTGCGCT ACACGAAATG CATTTTGACC 
GAAGGTTGCG GCAAGAACGG ATCATCGGTT GCTGGCACCA GTGTCCTCTC GCCCAGCATT 
CCGCTCACGC TGGTGGTGCT GCCCGCCCTG ATGATTGCCC AGAAGTCGCC GCAGAACCTC 
TTCACCGAGC ACGCATCGGT GTACATCATG GCGTTCGGCA TGGTGGCCGC CAAGGTCACC 
AATAAGCTGG TGATCGCTCA CATGACCAAG GCGGAGATGG AGTATCTGGA CTGGTCGCTG 
CTCGGCCCCT CGCTGCTGTT CCTCAACCAG TACTTCAACT GCATCGTGCC AGAGATCTGG 
CTACTGTGGT TCACGCTCAT CTGGGGCACC CAGGATCTGC TGCGCTACTG CGCCCAGGTG 
TGCCTCGAGA TTTGCCAGCA CCTGCGCATC GATCTCTTCC GGATACCGTA CACGCCCAAA 
GGCGCGACCC CGCATCCAGC CACCGCATCG GTGAGCAGCC AGAGCAACTT TGGCAGCAGT 
GCGGACAAGA ACGGTGGCAG CGCGCATCGA AAGTCGAAGA GCAAACCGCA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bbc ( NM_001274026.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260955.1 Drosophila melanogaster bb in a boxcar (bbc), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa13007
Clone ID Related Accession (Same CDS sequence)	NM_001274026.1
Accession Version	NM_001274026.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bb in a boxcar, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGCTGC TCGCCTACCG GGACAAGCAC ATCCTGAGTG CCCAGCAGCT GAGGAAGCTC 
AGCGAACACA AGTACTCCTG CTTCAGTGTC AGCCTGCTCG ATCCGCTGCT GCAGCCGTGG 
TGGAACTGGC TGGTCGCCCA GACGCCCCTT TGGCTGGCCC CCAATCTCAT TACCATCGTG 
GGACTGATCC TCAATGTGGT CACCACCCTG ATATTAATCT GTTATAGCCC AAATGGCGTT 
GAGGCTCCGC CGCGTTGGAC CTGCTTGCTC TGCGCGCTGG GTTTGTTCAT CTACCAGAGT 
CTGGACTCCA TCGACGGGAA GCAGGCGCGA CGCACGAATA CCTCATCGCC GTTGGGCGAA 
CTTTTCGACC ATGGCTGTGA CTCCATATCG ACGGTGTTTG TGGCCCTCTC GGCGTGCATC 
TCCTGCCAAC TGGGCCACTA TCCCAACTGG CTGTTCTTCC AGTGCTTCTG TGCTATTGCG 
CTCTTCTACT GCGCCCACTG GCAGACGTAT GTGTCTGGAA CGATGCGGTT CGGCAGAATT 
GATGTGACAG AGGCTCAGTT CTCAATTATA GCCATTCATT TGGTTTCGGC TGCACTGGGT 
CCTGAGATCT GGCTCACCAA GGGCACCAGT GTCCTCTCGC CCAGCATTCC GCTCACGCTG 
GTGGTGCTGC CCGCCCTGAT GATTGCCCAG AAGTCGCCGC AGAACCTCTT CACCGAGCAC 
GCATCGGTGT ACATCATGGC GTTCGGCATG GTGGCCGCCA AGGTCACCAA TAAGCTGGTG 
ATCGCTCACA TGACCAAGGC GGAGATGGAG TATCTGGACT GGTCGCTGCT CGGCCCCTCG 
CTGCTGTTCC TCAACCAGTA CTTCAACTGC ATCGTGCCAG AGATCTGGCT ACTGTGGTTC 
ACGCTCATCT GGGGCACCCA GGATCTGCTG CGCTACTGCG CCCAGGTGTG CCTCGAGATT 
TGCCAGCACC TGCGCATCGA TCTCTTCCGG ATACCGTACA CGCCCAAAGG CGCGACCCCG 
CATCCAGCCA CCGCATCGGT GAGCAGCCAG AGCAACTTTG GCAGCAGTGC GGACAAGAAC 
GGTGGCAGCG CGCATCGAAA GTCGAAGAGC AAACCGCACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260955.1
CDS	392..1513
Translation	MALLAYRDKHILSAQQLRKLSEHKYSCFSVSLLDPLLQPWWNWLVAQTPLWLAPNLITIVGLILNVVTTLILICYSPNGVEAPPRWTCLLCALGLFIYQSLDSIDGKQARRTNTSSPLGELFDHGCDSISTVFVALSACISCQLGHYPNWLFFQCFCAIALFYCAHWQTYVSGTMRFGRIDVTEAQFSIIAIHLVSAALGPEIWLTKGTSVLSPSIPLTLVVLPALMIAQKSPQNLFTEHASVYIMAFGMVAAKVTNKLVIAHMTKAEMEYLDWSLLGPSLLFLNQYFNCIVPEIWLLWFTLIWGTQDLLRYCAQVCLEICQHLRIDLFRIPYTPKGATPHPATASVSSQSNFGSSADKNGGSAHRKSKSKPH

Target ORF information:

RefSeq Version	NM_001274026.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bb in a boxcar (bbc), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274026.1

ORF Insert Sequence:

ATGGCGCTGC TCGCCTACCG GGACAAGCAC ATCCTGAGTG CCCAGCAGCT GAGGAAGCTC 
AGCGAACACA AGTACTCCTG CTTCAGTGTC AGCCTGCTCG ATCCGCTGCT GCAGCCGTGG 
TGGAACTGGC TGGTCGCCCA GACGCCCCTT TGGCTGGCCC CCAATCTCAT TACCATCGTG 
GGACTGATCC TCAATGTGGT CACCACCCTG ATATTAATCT GTTATAGCCC AAATGGCGTT 
GAGGCTCCGC CGCGTTGGAC CTGCTTGCTC TGCGCGCTGG GTTTGTTCAT CTACCAGAGT 
CTGGACTCCA TCGACGGGAA GCAGGCGCGA CGCACGAATA CCTCATCGCC GTTGGGCGAA 
CTTTTCGACC ATGGCTGTGA CTCCATATCG ACGGTGTTTG TGGCCCTCTC GGCGTGCATC 
TCCTGCCAAC TGGGCCACTA TCCCAACTGG CTGTTCTTCC AGTGCTTCTG TGCTATTGCG 
CTCTTCTACT GCGCCCACTG GCAGACGTAT GTGTCTGGAA CGATGCGGTT CGGCAGAATT 
GATGTGACAG AGGCTCAGTT CTCAATTATA GCCATTCATT TGGTTTCGGC TGCACTGGGT 
CCTGAGATCT GGCTCACCAA GGGCACCAGT GTCCTCTCGC CCAGCATTCC GCTCACGCTG 
GTGGTGCTGC CCGCCCTGAT GATTGCCCAG AAGTCGCCGC AGAACCTCTT CACCGAGCAC 
GCATCGGTGT ACATCATGGC GTTCGGCATG GTGGCCGCCA AGGTCACCAA TAAGCTGGTG 
ATCGCTCACA TGACCAAGGC GGAGATGGAG TATCTGGACT GGTCGCTGCT CGGCCCCTCG 
CTGCTGTTCC TCAACCAGTA CTTCAACTGC ATCGTGCCAG AGATCTGGCT ACTGTGGTTC 
ACGCTCATCT GGGGCACCCA GGATCTGCTG CGCTACTGCG CCCAGGTGTG CCTCGAGATT 
TGCCAGCACC TGCGCATCGA TCTCTTCCGG ATACCGTACA CGCCCAAAGG CGCGACCCCG 
CATCCAGCCA CCGCATCGGT GAGCAGCCAG AGCAACTTTG GCAGCAGTGC GGACAAGAAC 
GGTGGCAGCG CGCATCGAAA GTCGAAGAGC AAACCGCACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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bbc cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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