Nacalpha cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nacalpha
Entrez Gene ID	36409
Full Name	Nascent polypeptide associated complex protein alpha subunit
Synonyms	CG8759,Dmel\CG8759,NAC,NACalpha,aic,alpha-NAC,alphaNAC,l(2)04329
General protein information	Preferred Names Nascent polypeptide associated complex protein alpha subunit Names CG8759 gene product from transcript CG8759-RB CG8759-PA CG8759-PB CG8759-PC CG8759-PD NAC protein alpha subunit Nacalpha-PA Nacalpha-PB Nacalpha-PC Nacalpha-PD alpha NAC lethal (2) 04329 nascent polypeptide associated complex protein alpha subunit
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 49C2-49C3

mRNA and Protein(s)

mRNA	Protein	Name
NM_134312.3	NP_599139.1	nascent polypeptide associated complex protein alpha subunit, isoform A
NM_001299434.1	NP_001286363.1	nascent polypeptide associated complex protein alpha subunit, isoform D
NM_057868.4	NP_477216.1	nascent polypeptide associated complex protein alpha subunit, isoform B
NM_165950.2	NP_725229.1	nascent polypeptide associated complex protein alpha subunit, isoform C

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	naca	NP_775371.1
Eremothecium gossypii	AGOS_AER168C	NP_985026.1
Schizosaccharomyces pombe (fission yeast)	egd2	NP_596361.1
Magnaporthe oryzae (rice blast fungus)	MGG_02660	XP_003721118.1
Canis lupus familiaris (dog)	NACA	NP_001239186.1
Neurospora crassa	NCU00635	XP_965775.1
Arabidopsis thaliana (thale cress)	AT4G10480	NP_192786.1
Gallus gallus (chicken)	NACA	NP_001263232.1
Drosophila melanogaster (fruit fly)	Nacalpha	NP_599139.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006766	XP_308979.2
Saccharomyces cerevisiae (baker's yeast)	EGD2	NP_012063.3
Kluyveromyces lactis	KLLA0B04290g	XP_451723.1
Arabidopsis thaliana (thale cress)	NACA2	NP_190516.1
Pan troglodytes (chimpanzee)	NACA	XP_001168646.1
Bos taurus (cattle)	NACA	NP_001014916.1
Arabidopsis thaliana (thale cress)	NACA5	NP_564415.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nacalpha gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nacalpha cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa09815	NM_057868.4 Latest version!	Drosophila melanogaster nascent polypeptide associated complex protein alpha subunit, transcript variant B (Nacalpha), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa09815	NM_134312.3 Latest version!	Drosophila melanogaster nascent polypeptide associated complex protein alpha subunit, transcript variant A (Nacalpha), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa09815	NM_165950.2 Latest version!	Drosophila melanogaster nascent polypeptide associated complex protein alpha subunit, transcript variant C (Nacalpha), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa09815	NM_001299434.1 Latest version!	Drosophila melanogaster nascent polypeptide associated complex protein alpha subunit, transcript variant D (Nacalpha), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nacalpha ( NM_134312.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_599139.1 Drosophila melanogaster nascent polypeptide associated complex protein alpha subunit, transcript variant A (Nacalpha), mRNA.

Price & Availability↑

CloneID	OFa09815
Clone ID Related Accession (Same CDS sequence)	NM_134312.3 , NM_001299434.1 , NM_057868.4 , NM_165950.2
Accession Version	NM_134312.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nascent polypeptide associated complex protein alpha subunit, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_134312.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCGAAC TGACCGAAAT CAAGAGCGAG GCTGCGCCCT CCACCTCCGC TGAGGCGAAA 
CCCGAGGACG TCCGCGTTGA GGATGATGGC AGTGATAGCG ACTCCGATGG TGGCATGCCT 
GGTCTGGAGG AGGCTGTAGC GGCCACCACA CAGCTGGGTG GAGGTGCCAC CGGTCTGCCC 
ATCGACCTCG TCTCAAAGGC CAAGCAATCG CGCGGCGAGA AAAAGGCCAG GAAGATCATG 
CTGAAGCTGG GACTTAAGCA GATCCAGGGC GTCAACCGGG TGACGATCAG GAAGTCGAAG 
AACATCCTGT TCGTGATCAA CAACCCAGAT GTGTACAAGA ACCCCCACAG CGACACCTAC 
ATCGTGTTCG GTGAGGCCAA GATCGAGGAT CTGTCGCAAC AGGCCCAGGT GGCTGCCGCC 
GAGAAGTTCA AGGCCCCGGA GGCTGCTGGC GCTGCAGACA GCGTGGGCGC CACCACATCG 
GTGGCCCCCA TTGCCGAGGA AGACGAGGAG GATGTCGATG ACACCGGCGT GGATGAGAAG 
GATATCGAGC TGGTCATCAC GCAGGCCAAC ACGACGAGGG CCAAGGCGAT CAAGGCTCTG 
AAGAACAACA ACAATGACAT CGTCAACGCC ATCATGGAGC TTACTATGCT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_599139.1
CDS	73..726
Translation	MPELTEIKSEAAPSTSAEAKPEDVRVEDDGSDSDSDGGMPGLEEAVAATTQLGGGATGLPIDLVSKAKQSRGEKKARKIMLKLGLKQIQGVNRVTIRKSKNILFVINNPDVYKNPHSDTYIVFGEAKIEDLSQQAQVAAAEKFKAPEAAGAADSVGATTSVAPIAEEDEEDVDDTGVDEKDIELVITQANTTRAKAIKALKNNNNDIVNAIMELTML

Target ORF information:

RefSeq Version	NM_134312.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nascent polypeptide associated complex protein alpha subunit, transcript variant A (Nacalpha), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134312.3

ORF Insert Sequence:

ATGCCCGAAC TGACCGAAAT CAAGAGCGAG GCTGCGCCCT CCACCTCCGC TGAGGCGAAA 
CCCGAGGACG TCCGCGTTGA GGATGATGGC AGTGATAGCG ACTCCGATGG TGGCATGCCT 
GGTCTGGAGG AGGCTGTAGC GGCCACCACA CAGCTGGGTG GAGGTGCCAC CGGTCTGCCC 
ATCGACCTCG TCTCAAAGGC CAAGCAATCG CGCGGCGAGA AAAAGGCCAG GAAGATCATG 
CTGAAGCTGG GACTTAAGCA GATCCAGGGC GTCAACCGGG TGACGATCAG GAAGTCGAAG 
AACATCCTGT TCGTGATCAA CAACCCAGAT GTGTACAAGA ACCCCCACAG CGACACCTAC 
ATCGTGTTCG GTGAGGCCAA GATCGAGGAT CTGTCGCAAC AGGCCCAGGT GGCTGCCGCC 
GAGAAGTTCA AGGCCCCGGA GGCTGCTGGC GCTGCAGACA GCGTGGGCGC CACCACATCG 
GTGGCCCCCA TTGCCGAGGA AGACGAGGAG GATGTCGATG ACACCGGCGT GGATGAGAAG 
GATATCGAGC TGGTCATCAC GCAGGCCAAC ACGACGAGGG CCAAGGCGAT CAAGGCTCTG 
AAGAACAACA ACAATGACAT CGTCAACGCC ATCATGGAGC TTACTATGCT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nacalpha ( NM_001299434.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286363.1 Drosophila melanogaster nascent polypeptide associated complex protein alpha subunit, transcript variant D (Nacalpha), mRNA.

Price & Availability↑

CloneID	OFa09815
Clone ID Related Accession (Same CDS sequence)	NM_134312.3 , NM_001299434.1 , NM_057868.4 , NM_165950.2
Accession Version	NM_001299434.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nascent polypeptide associated complex protein alpha subunit, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCGAAC TGACCGAAAT CAAGAGCGAG GCTGCGCCCT CCACCTCCGC TGAGGCGAAA 
CCCGAGGACG TCCGCGTTGA GGATGATGGC AGTGATAGCG ACTCCGATGG TGGCATGCCT 
GGTCTGGAGG AGGCTGTAGC GGCCACCACA CAGCTGGGTG GAGGTGCCAC CGGTCTGCCC 
ATCGACCTCG TCTCAAAGGC CAAGCAATCG CGCGGCGAGA AAAAGGCCAG GAAGATCATG 
CTGAAGCTGG GACTTAAGCA GATCCAGGGC GTCAACCGGG TGACGATCAG GAAGTCGAAG 
AACATCCTGT TCGTGATCAA CAACCCAGAT GTGTACAAGA ACCCCCACAG CGACACCTAC 
ATCGTGTTCG GTGAGGCCAA GATCGAGGAT CTGTCGCAAC AGGCCCAGGT GGCTGCCGCC 
GAGAAGTTCA AGGCCCCGGA GGCTGCTGGC GCTGCAGACA GCGTGGGCGC CACCACATCG 
GTGGCCCCCA TTGCCGAGGA AGACGAGGAG GATGTCGATG ACACCGGCGT GGATGAGAAG 
GATATCGAGC TGGTCATCAC GCAGGCCAAC ACGACGAGGG CCAAGGCGAT CAAGGCTCTG 
AAGAACAACA ACAATGACAT CGTCAACGCC ATCATGGAGC TTACTATGCT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286363.1
CDS	10..663
Translation	MPELTEIKSEAAPSTSAEAKPEDVRVEDDGSDSDSDGGMPGLEEAVAATTQLGGGATGLPIDLVSKAKQSRGEKKARKIMLKLGLKQIQGVNRVTIRKSKNILFVINNPDVYKNPHSDTYIVFGEAKIEDLSQQAQVAAAEKFKAPEAAGAADSVGATTSVAPIAEEDEEDVDDTGVDEKDIELVITQANTTRAKAIKALKNNNNDIVNAIMELTML

Target ORF information:

RefSeq Version	NM_001299434.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nascent polypeptide associated complex protein alpha subunit, transcript variant D (Nacalpha), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299434.1

ORF Insert Sequence:

ATGCCCGAAC TGACCGAAAT CAAGAGCGAG GCTGCGCCCT CCACCTCCGC TGAGGCGAAA 
CCCGAGGACG TCCGCGTTGA GGATGATGGC AGTGATAGCG ACTCCGATGG TGGCATGCCT 
GGTCTGGAGG AGGCTGTAGC GGCCACCACA CAGCTGGGTG GAGGTGCCAC CGGTCTGCCC 
ATCGACCTCG TCTCAAAGGC CAAGCAATCG CGCGGCGAGA AAAAGGCCAG GAAGATCATG 
CTGAAGCTGG GACTTAAGCA GATCCAGGGC GTCAACCGGG TGACGATCAG GAAGTCGAAG 
AACATCCTGT TCGTGATCAA CAACCCAGAT GTGTACAAGA ACCCCCACAG CGACACCTAC 
ATCGTGTTCG GTGAGGCCAA GATCGAGGAT CTGTCGCAAC AGGCCCAGGT GGCTGCCGCC 
GAGAAGTTCA AGGCCCCGGA GGCTGCTGGC GCTGCAGACA GCGTGGGCGC CACCACATCG 
GTGGCCCCCA TTGCCGAGGA AGACGAGGAG GATGTCGATG ACACCGGCGT GGATGAGAAG 
GATATCGAGC TGGTCATCAC GCAGGCCAAC ACGACGAGGG CCAAGGCGAT CAAGGCTCTG 
AAGAACAACA ACAATGACAT CGTCAACGCC ATCATGGAGC TTACTATGCT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nacalpha ( NM_057868.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477216.1 Drosophila melanogaster nascent polypeptide associated complex protein alpha subunit, transcript variant B (Nacalpha), mRNA.

Price & Availability↑

CloneID	OFa09815
Clone ID Related Accession (Same CDS sequence)	NM_134312.3 , NM_001299434.1 , NM_057868.4 , NM_165950.2
Accession Version	NM_057868.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nascent polypeptide associated complex protein alpha subunit, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_057868.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCGAAC TGACCGAAAT CAAGAGCGAG GCTGCGCCCT CCACCTCCGC TGAGGCGAAA 
CCCGAGGACG TCCGCGTTGA GGATGATGGC AGTGATAGCG ACTCCGATGG TGGCATGCCT 
GGTCTGGAGG AGGCTGTAGC GGCCACCACA CAGCTGGGTG GAGGTGCCAC CGGTCTGCCC 
ATCGACCTCG TCTCAAAGGC CAAGCAATCG CGCGGCGAGA AAAAGGCCAG GAAGATCATG 
CTGAAGCTGG GACTTAAGCA GATCCAGGGC GTCAACCGGG TGACGATCAG GAAGTCGAAG 
AACATCCTGT TCGTGATCAA CAACCCAGAT GTGTACAAGA ACCCCCACAG CGACACCTAC 
ATCGTGTTCG GTGAGGCCAA GATCGAGGAT CTGTCGCAAC AGGCCCAGGT GGCTGCCGCC 
GAGAAGTTCA AGGCCCCGGA GGCTGCTGGC GCTGCAGACA GCGTGGGCGC CACCACATCG 
GTGGCCCCCA TTGCCGAGGA AGACGAGGAG GATGTCGATG ACACCGGCGT GGATGAGAAG 
GATATCGAGC TGGTCATCAC GCAGGCCAAC ACGACGAGGG CCAAGGCGAT CAAGGCTCTG 
AAGAACAACA ACAATGACAT CGTCAACGCC ATCATGGAGC TTACTATGCT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477216.1
CDS	202..855
Translation	MPELTEIKSEAAPSTSAEAKPEDVRVEDDGSDSDSDGGMPGLEEAVAATTQLGGGATGLPIDLVSKAKQSRGEKKARKIMLKLGLKQIQGVNRVTIRKSKNILFVINNPDVYKNPHSDTYIVFGEAKIEDLSQQAQVAAAEKFKAPEAAGAADSVGATTSVAPIAEEDEEDVDDTGVDEKDIELVITQANTTRAKAIKALKNNNNDIVNAIMELTML

Target ORF information:

RefSeq Version	NM_057868.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nascent polypeptide associated complex protein alpha subunit, transcript variant B (Nacalpha), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057868.4

ORF Insert Sequence:

ATGCCCGAAC TGACCGAAAT CAAGAGCGAG GCTGCGCCCT CCACCTCCGC TGAGGCGAAA 
CCCGAGGACG TCCGCGTTGA GGATGATGGC AGTGATAGCG ACTCCGATGG TGGCATGCCT 
GGTCTGGAGG AGGCTGTAGC GGCCACCACA CAGCTGGGTG GAGGTGCCAC CGGTCTGCCC 
ATCGACCTCG TCTCAAAGGC CAAGCAATCG CGCGGCGAGA AAAAGGCCAG GAAGATCATG 
CTGAAGCTGG GACTTAAGCA GATCCAGGGC GTCAACCGGG TGACGATCAG GAAGTCGAAG 
AACATCCTGT TCGTGATCAA CAACCCAGAT GTGTACAAGA ACCCCCACAG CGACACCTAC 
ATCGTGTTCG GTGAGGCCAA GATCGAGGAT CTGTCGCAAC AGGCCCAGGT GGCTGCCGCC 
GAGAAGTTCA AGGCCCCGGA GGCTGCTGGC GCTGCAGACA GCGTGGGCGC CACCACATCG 
GTGGCCCCCA TTGCCGAGGA AGACGAGGAG GATGTCGATG ACACCGGCGT GGATGAGAAG 
GATATCGAGC TGGTCATCAC GCAGGCCAAC ACGACGAGGG CCAAGGCGAT CAAGGCTCTG 
AAGAACAACA ACAATGACAT CGTCAACGCC ATCATGGAGC TTACTATGCT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nacalpha ( NM_165950.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725229.1 Drosophila melanogaster nascent polypeptide associated complex protein alpha subunit, transcript variant C (Nacalpha), mRNA.

Price & Availability↑

CloneID	OFa09815
Clone ID Related Accession (Same CDS sequence)	NM_134312.3 , NM_001299434.1 , NM_057868.4 , NM_165950.2
Accession Version	NM_165950.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nascent polypeptide associated complex protein alpha subunit, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_165950.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCGAAC TGACCGAAAT CAAGAGCGAG GCTGCGCCCT CCACCTCCGC TGAGGCGAAA 
CCCGAGGACG TCCGCGTTGA GGATGATGGC AGTGATAGCG ACTCCGATGG TGGCATGCCT 
GGTCTGGAGG AGGCTGTAGC GGCCACCACA CAGCTGGGTG GAGGTGCCAC CGGTCTGCCC 
ATCGACCTCG TCTCAAAGGC CAAGCAATCG CGCGGCGAGA AAAAGGCCAG GAAGATCATG 
CTGAAGCTGG GACTTAAGCA GATCCAGGGC GTCAACCGGG TGACGATCAG GAAGTCGAAG 
AACATCCTGT TCGTGATCAA CAACCCAGAT GTGTACAAGA ACCCCCACAG CGACACCTAC 
ATCGTGTTCG GTGAGGCCAA GATCGAGGAT CTGTCGCAAC AGGCCCAGGT GGCTGCCGCC 
GAGAAGTTCA AGGCCCCGGA GGCTGCTGGC GCTGCAGACA GCGTGGGCGC CACCACATCG 
GTGGCCCCCA TTGCCGAGGA AGACGAGGAG GATGTCGATG ACACCGGCGT GGATGAGAAG 
GATATCGAGC TGGTCATCAC GCAGGCCAAC ACGACGAGGG CCAAGGCGAT CAAGGCTCTG 
AAGAACAACA ACAATGACAT CGTCAACGCC ATCATGGAGC TTACTATGCT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725229.1
CDS	43..696
Translation	MPELTEIKSEAAPSTSAEAKPEDVRVEDDGSDSDSDGGMPGLEEAVAATTQLGGGATGLPIDLVSKAKQSRGEKKARKIMLKLGLKQIQGVNRVTIRKSKNILFVINNPDVYKNPHSDTYIVFGEAKIEDLSQQAQVAAAEKFKAPEAAGAADSVGATTSVAPIAEEDEEDVDDTGVDEKDIELVITQANTTRAKAIKALKNNNNDIVNAIMELTML

Target ORF information:

RefSeq Version	NM_165950.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nascent polypeptide associated complex protein alpha subunit, transcript variant C (Nacalpha), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165950.2

ORF Insert Sequence:

ATGCCCGAAC TGACCGAAAT CAAGAGCGAG GCTGCGCCCT CCACCTCCGC TGAGGCGAAA 
CCCGAGGACG TCCGCGTTGA GGATGATGGC AGTGATAGCG ACTCCGATGG TGGCATGCCT 
GGTCTGGAGG AGGCTGTAGC GGCCACCACA CAGCTGGGTG GAGGTGCCAC CGGTCTGCCC 
ATCGACCTCG TCTCAAAGGC CAAGCAATCG CGCGGCGAGA AAAAGGCCAG GAAGATCATG 
CTGAAGCTGG GACTTAAGCA GATCCAGGGC GTCAACCGGG TGACGATCAG GAAGTCGAAG 
AACATCCTGT TCGTGATCAA CAACCCAGAT GTGTACAAGA ACCCCCACAG CGACACCTAC 
ATCGTGTTCG GTGAGGCCAA GATCGAGGAT CTGTCGCAAC AGGCCCAGGT GGCTGCCGCC 
GAGAAGTTCA AGGCCCCGGA GGCTGCTGGC GCTGCAGACA GCGTGGGCGC CACCACATCG 
GTGGCCCCCA TTGCCGAGGA AGACGAGGAG GATGTCGATG ACACCGGCGT GGATGAGAAG 
GATATCGAGC TGGTCATCAC GCAGGCCAAC ACGACGAGGG CCAAGGCGAT CAAGGCTCTG 
AAGAACAACA ACAATGACAT CGTCAACGCC ATCATGGAGC TTACTATGCT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nacalpha cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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