ADD1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ADD1
Entrez Gene ID	36278
Full Name	ADD domain-containing protein 1
Synonyms	BEST:LD29214,CG8290,Dmel\CG8290,LD29214,dADD1,dadd1
General protein information	Preferred Names ADD domain-containing protein 1 Names CG8290 gene product from transcript CG8290-RA ADD1-PA ADD1-PB ADD1-PE CG8290-PA CG8290-PB CG8290-PE
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 48D5-48D5

mRNA and Protein(s)

mRNA	Protein	Name
NM_165856.2	NP_725094.1	ADD domain-containing protein 1, isoform A
NM_136872.3	NP_610716.1	ADD domain-containing protein 1, isoform B
NM_001299394.1	NP_001286323.1	ADD domain-containing protein 1, isoform E

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

dadd1-encoded proteins participate with dAtrx in some cellular functions such as heterochromatin maintenance
Title: Characterization of the Drosophila group ortholog to the amino-terminus of the alpha-thalassemia and mental retardation X-Linked (ATRX) vertebrate protein.

The following ADD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ADD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa13286	NM_001299394.1 Latest version!	Drosophila melanogaster ADD domain-containing protein 1 (ADD1), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa09661	NM_165856.2 Latest version!	Drosophila melanogaster ADD domain-containing protein 1 (ADD1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa09662	NM_136872.3 Latest version!	Drosophila melanogaster ADD domain-containing protein 1 (ADD1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ADD1 ( NM_001299394.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286323.1 Drosophila melanogaster ADD domain-containing protein 1 (ADD1), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa13286
Clone ID Related Accession (Same CDS sequence)	NM_001299394.1
Accession Version	NM_001299394.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2940bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ADD domain-containing protein 1, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTAACA GTGCTCCGGG TAGCGAAAGT GGCCTGGCAA AGGTAGCGAC GCCGTCGCCA 
TCGCCACTGG CCAAATTGGA TGCGGATTTC ACGAGCTCCA AGTTCGAGAT GCACCCCTCG 
CTTTCAGACG AGGAGCGCCG CTTCTACCTG AAGATGTACC CCAACGTAGA TCTGGTGAAC 
CAGCGAAAGG TGCACTGCAC CGTGTGCAAG CTTCACCTGG GCACAGCACC CGCCGCCGAG 
AGCAACATCA AGATGCATCC CATACTGCGG GTGACGCACT GCGTCAAGTG CCACGATTTT 
TACAACAGCG GCGAGTTCTC CAAGGGCGAG GATGGCTCTG AGCTGTACTG CCGCTGGTGC 
GGACAGGGCG GCGAGGTATA CTGCTGCTCC ACTTGCCCCT ACGTCTTCTG CAAGTCGTGC 
ATCGTGAAGA ACCTCTCCAA GGGCGTCATC GTGGACATCG AGCAAAACGA GAACTGGAAC 
TGCTTCAGCT GCACCTCCAA GATCCTGTGG CCACTACGAG CCCAACACTG GGCGCTGGTC 
AACTATCTTC AGACGCAGAA AAGGGCCCTA CAAACCCTTC AGCTTCCCGA GGTGGAGGCC 
CGACGTCAAA TGCTTAAGGA TAACAGCAAT TGCTGTCGCT TGGCGAAGTC GAAAACCAGC 
AGCTTATCCG ACTCGATGGA AAGCTTGGAG TCCAATGTCT CCAAACGCAG CCAGGGCAGC 
TCAGCGGGCT CGAGTAAACG GGGATCCATG CCAAAAACCA TTCCTCCGGG ACCACCATCC 
AAGCGCGCTA AAGCTTCCAA CAACGAAGTG GTCTGCACAC CGGATCTGCT CAGCATGCTG 
GAGCCGGATT GCCAGCTCTC TGTGACGCCC AAGCCTACTG GACGTCAGAC GGTGACAGCG 
GCAAATATCA CCACTCCGAG GATTGTCACC GTCCAACAGA ACTACCAACC GGGACAAAGT 
TCCAGCAACA GCGGCAGCAA CGGAAGCACG AACCAACAGC AGCGGGCTCC GCAAGTGCGA 
GTTGGAAACG CCAATCCAGC GCCTCCACCG CTGCAAGGAC TTCCGCCCAA TGCCAATGGC 
GGTCAAACTC TCATGATTAC TCCAGCACCA CAACCAGCGC CTAAGGTTCC AACTTTGGTG 
CGTCTCACCT TCCCCAACAC GCCGATGGGT CAGGCACGGG CGCAGCTGCA GGAGCAAATC 
TTTAGCGCCA TGGACATCTG CACTCATCTT ACGGGCAAAG TGGTATCCCT TACCCACTCG 
AACGCCTACA ACCAGGTTCG GAGCTATCTG GAACTGAAGG AGCTATACAT TCACATGTCC 
TATTTGATGA CCTATGCGAT TGGGCGGTTT AAGAACCTGC AGGAGAAGTG TTTGGTGGAC 
ATGCGAGAGA AGGGCTTCAA GAATGACGCC AACAGTCTGG AGAATGGTCA ACTAGCTGCT 
GCCGTGGATT ATGTGTGTTA CGGCCAACTG GACGACGTGG ATCTCTTCAA CACAGGCTGC 
AATAGCTTTC ACAATCAGGT CTATGAATAC CGCAAGAGTT TGCATGCCAA TCTTAAGGAT 
GGCGAGGACT GCGAGCCATT GCCTCCGCTG ATGCCGCTGG GAGTTCGAGC CGAAGGTGAT 
CCCATCGATG AGCCCGAGGA ACCGGAAACG GTACCGGAAC CGCAGCAGGA TGACACCGAA 
AAAGACCACG AAGATGCGGC CGAATTCGAT TATGATGAGC CAGAGGATTT GGATGATGAT 
TTGGATGATG ATGACTTGGA CAACGAGGAG TGTGGAGAAC TGGGCACCGA AGAGAGCATG 
AATCGGAATG AACAAGCTCA CGCCTACGAT CGAAAGTTGA CACGCCTTCT CCGCGAGTAT 
CCTTCGTTGT GGTGCACCCG TCATCCCGAT TACGGCAAGA AAGAGGTCAC TAATAAGCAG 
TGGCGGGTGA TTGCCAGTCA TTTTCCACGC GGTGATGACA TTAAGCTGCG CTGGAAGAAC 
GTAAGGAAGC GATACGTTCG GATTGAGAGG CGCTTAAAGC AGGGAAAGCG TTTTAAAGGC 
TACTTCGACA AAGCGACAAA CTATTTGGAG AACAGGGATC GCCCGAGGAG CGAGTGGTTG 
GCGGTGGACT GTGGCGAAGA TGACGGCGAT GGCTTTGAGC GCAAGCACCT CGACGTAATA 
GAGAATAAGA TGGAGCAAAA AGCTCATAAG CAGCCAGCAG TTAAAATCAA CCTAAAAGGT 
GGATCTAGAA TACCGACGCG ACCACTCGAG GTGCGTCCCA TTGATCAACG CATCATAACT 
TTCGCTAAAA GCCATCCAGT ATTATGGCGC AAATCCGCTG ATCCCGAATT CAACAGTATT 
GACGAGCAAA CTAGAAAGTC GCTGTGGCAA AACTTTTGGA AGTCAGCGCC CAATTATGCT 
TGCGAGTATC TTGTGGAGCG CTGGCAGCAG ATGTACGAGA TGTACAAGTC CTTCCGCCTG 
AAGAGTATTA AAGATAAGGG AATCTTTGCG AATCTGGAGC TAAAGTACTC AAAGTACTTG 
GCCAGCTTGT ATTTCTTGTA CAAGATCGAT GAGCAGGACC TGCGCGATGA GTTTGAACCG 
CTGCAGGACT GTGTCCCGAG CGGCACTGAC TCCGCTGGAA ACCTAAAAGA TATGCCCGAT 
GACAAGCAGT TCTGTCAGCA ACTGGTTTTG GCTATGCGTG CATATCCAAC ACTTTGGAAT 
CCACGACATG TTGACTACAG CGATGTGGGA GCCAGGGAGC GCTTATGGCC GGAAGTGGCC 
AGGCGGTTAC CCCGATTCAA GCGGGACGCT CAGGCCTGTA AGCTTCGCTG GCAGATGGCC 
AAGTTCGCTT ACGAGTGTTA CCTACGCGAG ATGGAGCGCC ATCCGCGTGT CAACGAACGA 
AAGTTGCAGA AGTTGCGCTC CAACTTTCCG CTCGAGGAGA TGCGCTTCCT CAACATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286323.1
CDS	297..3236
Translation	MSNSAPGSESGLAKVATPSPSPLAKLDADFTSSKFEMHPSLSDEERRFYLKMYPNVDLVNQRKVHCTVCKLHLGTAPAAESNIKMHPILRVTHCVKCHDFYNSGEFSKGEDGSELYCRWCGQGGEVYCCSTCPYVFCKSCIVKNLSKGVIVDIEQNENWNCFSCTSKILWPLRAQHWALVNYLQTQKRALQTLQLPEVEARRQMLKDNSNCCRLAKSKTSSLSDSMESLESNVSKRSQGSSAGSSKRGSMPKTIPPGPPSKRAKASNNEVVCTPDLLSMLEPDCQLSVTPKPTGRQTVTAANITTPRIVTVQQNYQPGQSSSNSGSNGSTNQQQRAPQVRVGNANPAPPPLQGLPPNANGGQTLMITPAPQPAPKVPTLVRLTFPNTPMGQARAQLQEQIFSAMDICTHLTGKVVSLTHSNAYNQVRSYLELKELYIHMSYLMTYAIGRFKNLQEKCLVDMREKGFKNDANSLENGQLAAAVDYVCYGQLDDVDLFNTGCNSFHNQVYEYRKSLHANLKDGEDCEPLPPLMPLGVRAEGDPIDEPEEPETVPEPQQDDTEKDHEDAAEFDYDEPEDLDDDLDDDDLDNEECGELGTEESMNRNEQAHAYDRKLTRLLREYPSLWCTRHPDYGKKEVTNKQWRVIASHFPRGDDIKLRWKNVRKRYVRIERRLKQGKRFKGYFDKATNYLENRDRPRSEWLAVDCGEDDGDGFERKHLDVIENKMEQKAHKQPAVKINLKGGSRIPTRPLEVRPIDQRIITFAKSHPVLWRKSADPEFNSIDEQTRKSLWQNFWKSAPNYACEYLVERWQQMYEMYKSFRLKSIKDKGIFANLELKYSKYLASLYFLYKIDEQDLRDEFEPLQDCVPSGTDSAGNLKDMPDDKQFCQQLVLAMRAYPTLWNPRHVDYSDVGARERLWPEVARRLPRFKRDAQACKLRWQMAKFAYECYLREMERHPRVNERKLQKLRSNFPLEEMRFLNI

Target ORF information:

RefSeq Version	NM_001299394.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ADD domain-containing protein 1 (ADD1), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299394.1

ORF Insert Sequence:

ATGAGTAACA GTGCTCCGGG TAGCGAAAGT GGCCTGGCAA AGGTAGCGAC GCCGTCGCCA 
TCGCCACTGG CCAAATTGGA TGCGGATTTC ACGAGCTCCA AGTTCGAGAT GCACCCCTCG 
CTTTCAGACG AGGAGCGCCG CTTCTACCTG AAGATGTACC CCAACGTAGA TCTGGTGAAC 
CAGCGAAAGG TGCACTGCAC CGTGTGCAAG CTTCACCTGG GCACAGCACC CGCCGCCGAG 
AGCAACATCA AGATGCATCC CATACTGCGG GTGACGCACT GCGTCAAGTG CCACGATTTT 
TACAACAGCG GCGAGTTCTC CAAGGGCGAG GATGGCTCTG AGCTGTACTG CCGCTGGTGC 
GGACAGGGCG GCGAGGTATA CTGCTGCTCC ACTTGCCCCT ACGTCTTCTG CAAGTCGTGC 
ATCGTGAAGA ACCTCTCCAA GGGCGTCATC GTGGACATCG AGCAAAACGA GAACTGGAAC 
TGCTTCAGCT GCACCTCCAA GATCCTGTGG CCACTACGAG CCCAACACTG GGCGCTGGTC 
AACTATCTTC AGACGCAGAA AAGGGCCCTA CAAACCCTTC AGCTTCCCGA GGTGGAGGCC 
CGACGTCAAA TGCTTAAGGA TAACAGCAAT TGCTGTCGCT TGGCGAAGTC GAAAACCAGC 
AGCTTATCCG ACTCGATGGA AAGCTTGGAG TCCAATGTCT CCAAACGCAG CCAGGGCAGC 
TCAGCGGGCT CGAGTAAACG GGGATCCATG CCAAAAACCA TTCCTCCGGG ACCACCATCC 
AAGCGCGCTA AAGCTTCCAA CAACGAAGTG GTCTGCACAC CGGATCTGCT CAGCATGCTG 
GAGCCGGATT GCCAGCTCTC TGTGACGCCC AAGCCTACTG GACGTCAGAC GGTGACAGCG 
GCAAATATCA CCACTCCGAG GATTGTCACC GTCCAACAGA ACTACCAACC GGGACAAAGT 
TCCAGCAACA GCGGCAGCAA CGGAAGCACG AACCAACAGC AGCGGGCTCC GCAAGTGCGA 
GTTGGAAACG CCAATCCAGC GCCTCCACCG CTGCAAGGAC TTCCGCCCAA TGCCAATGGC 
GGTCAAACTC TCATGATTAC TCCAGCACCA CAACCAGCGC CTAAGGTTCC AACTTTGGTG 
CGTCTCACCT TCCCCAACAC GCCGATGGGT CAGGCACGGG CGCAGCTGCA GGAGCAAATC 
TTTAGCGCCA TGGACATCTG CACTCATCTT ACGGGCAAAG TGGTATCCCT TACCCACTCG 
AACGCCTACA ACCAGGTTCG GAGCTATCTG GAACTGAAGG AGCTATACAT TCACATGTCC 
TATTTGATGA CCTATGCGAT TGGGCGGTTT AAGAACCTGC AGGAGAAGTG TTTGGTGGAC 
ATGCGAGAGA AGGGCTTCAA GAATGACGCC AACAGTCTGG AGAATGGTCA ACTAGCTGCT 
GCCGTGGATT ATGTGTGTTA CGGCCAACTG GACGACGTGG ATCTCTTCAA CACAGGCTGC 
AATAGCTTTC ACAATCAGGT CTATGAATAC CGCAAGAGTT TGCATGCCAA TCTTAAGGAT 
GGCGAGGACT GCGAGCCATT GCCTCCGCTG ATGCCGCTGG GAGTTCGAGC CGAAGGTGAT 
CCCATCGATG AGCCCGAGGA ACCGGAAACG GTACCGGAAC CGCAGCAGGA TGACACCGAA 
AAAGACCACG AAGATGCGGC CGAATTCGAT TATGATGAGC CAGAGGATTT GGATGATGAT 
TTGGATGATG ATGACTTGGA CAACGAGGAG TGTGGAGAAC TGGGCACCGA AGAGAGCATG 
AATCGGAATG AACAAGCTCA CGCCTACGAT CGAAAGTTGA CACGCCTTCT CCGCGAGTAT 
CCTTCGTTGT GGTGCACCCG TCATCCCGAT TACGGCAAGA AAGAGGTCAC TAATAAGCAG 
TGGCGGGTGA TTGCCAGTCA TTTTCCACGC GGTGATGACA TTAAGCTGCG CTGGAAGAAC 
GTAAGGAAGC GATACGTTCG GATTGAGAGG CGCTTAAAGC AGGGAAAGCG TTTTAAAGGC 
TACTTCGACA AAGCGACAAA CTATTTGGAG AACAGGGATC GCCCGAGGAG CGAGTGGTTG 
GCGGTGGACT GTGGCGAAGA TGACGGCGAT GGCTTTGAGC GCAAGCACCT CGACGTAATA 
GAGAATAAGA TGGAGCAAAA AGCTCATAAG CAGCCAGCAG TTAAAATCAA CCTAAAAGGT 
GGATCTAGAA TACCGACGCG ACCACTCGAG GTGCGTCCCA TTGATCAACG CATCATAACT 
TTCGCTAAAA GCCATCCAGT ATTATGGCGC AAATCCGCTG ATCCCGAATT CAACAGTATT 
GACGAGCAAA CTAGAAAGTC GCTGTGGCAA AACTTTTGGA AGTCAGCGCC CAATTATGCT 
TGCGAGTATC TTGTGGAGCG CTGGCAGCAG ATGTACGAGA TGTACAAGTC CTTCCGCCTG 
AAGAGTATTA AAGATAAGGG AATCTTTGCG AATCTGGAGC TAAAGTACTC AAAGTACTTG 
GCCAGCTTGT ATTTCTTGTA CAAGATCGAT GAGCAGGACC TGCGCGATGA GTTTGAACCG 
CTGCAGGACT GTGTCCCGAG CGGCACTGAC TCCGCTGGAA ACCTAAAAGA TATGCCCGAT 
GACAAGCAGT TCTGTCAGCA ACTGGTTTTG GCTATGCGTG CATATCCAAC ACTTTGGAAT 
CCACGACATG TTGACTACAG CGATGTGGGA GCCAGGGAGC GCTTATGGCC GGAAGTGGCC 
AGGCGGTTAC CCCGATTCAA GCGGGACGCT CAGGCCTGTA AGCTTCGCTG GCAGATGGCC 
AAGTTCGCTT ACGAGTGTTA CCTACGCGAG ATGGAGCGCC ATCCGCGTGT CAACGAACGA 
AAGTTGCAGA AGTTGCGCTC CAACTTTCCG CTCGAGGAGA TGCGCTTCCT CAACATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADD1 ( NM_165856.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725094.1 Drosophila melanogaster ADD domain-containing protein 1 (ADD1), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa09661
Clone ID Related Accession (Same CDS sequence)	NM_165856.2
Accession Version	NM_165856.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ADD domain-containing protein 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165856.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTAACA GTGCTCCGGG TAGCGAAAGT GGCCTGGCAA AGGTAGCGAC GCCGTCGCCA 
TCGCCACTGG CCAAATTGGA TGCGGATTTC ACGAGCTCCA AGTTCGAGAT GCACCCCTCG 
CTTTCAGACG AGGAGCGCCG CTTCTACCTG AAGATGTACC CCAACGTAGA TCTGGTGAAC 
CAGCGAAAGG TGCACTGCAC CGTGTGCAAG CTTCACCTGG GCACAGCACC CGCCGCCGAG 
AGCAACATCA AGATGCATCC CATACTGCGG GTGACGCACT GCGTCAAGTG CCACGATTTT 
TACAACAGCG GCGAGTTCTC CAAGGGCGAG GATGGCTCTG AGCTGTACTG CCGCTGGTGC 
GGACAGGGCG GCGAGGTATA CTGCTGCTCC ACTTGCCCCT ACGTCTTCTG CAAGTCGTGC 
ATCGTGAAGA ACCTCTCCAA GGGCGTCATC GTGGACATCG AGCAAAACGA GAACTGGAAC 
TGCTTCAGCT GCACCTCCAA GATCCTGTGG CCACTACGAG CCCAACACTG GGCGCTGGTC 
AACTATCTTC AGACGCAGAA AAGGGCCCTA CAAACCCTTC AGCTTCCCGA GGTGGAGGCC 
CGACGTCAAA TGCTTAAGGA TAACAGCAAT TGCTGTCGCT TGGCGAAGTC GAAAACCAGC 
AGCTTATCCG ACTCGATGGA AAGCTTGGAG TCCAATGTCT CCAAACGCAG CCAGGGCAGC 
TCAGCGGGCT CGAGTAAACG GGGATCCATG CCAAAAACCA TTCCTCCGGG ACCACCATCC 
AAGCGCGCTA AAGCTTCCAA CAACGAAGTG GTCTGCACAC CGGATCTGCT CAGCATGCTG 
GAGCCGGATT GCCAGCTCTC TGTGACGCCC AAGCCTACTG GACGTCAGAC GGTGACAGCG 
GCAAATATCA CCACTCCGAG GATTGTCACC GTCCAACAGA ACTACCAACC GGGACAAAGT 
TCCAGCAACA GCGGCAGCAA CGGAAGCGTA AGTGGTTCTT CATCAGTGCC ACCGACACGT 
AGTAGCCTGC CACCTCCCTT GGTTCTCAGT GGATCTGGAA TCCGCTACCG CCAGAATGCC 
CCTGGTGGGG TCTCTGGTGT GGTACGTCGT ACTGTGGTTC CCAACTCTGT AAAAACCACT 
GGGCCAGTCT TTCACACAAT CAATGGGTTC AAAGTAGATT TAAATAGCGC TGCACAACAG 
GATTCCTATC GCCTGCCCAA TGGTAGACTA ATTCAGGTAA AACGTCAGGT ACCACAGACA 
ATTCAGCCGG CATCAACAGT ACCAACACCA CCAACACCGC CTGCTTTGGT CGTTCCTCAA 
GTGGGTCCGG GTGTGGTTAT CCGACCTCGA GCTCCAATTA CGACTAATAG ACCTTCACTG 
CATATCAGCA CGTACGGTTC TAACATGGGT ATCTATAACC CGCAGACGAA CCAACAGCAG 
CGGGCTCCGC AAGTGCGAGT TGGAAACGCC AATCCAGCGC CTCCACCGCT GCAAGGACTT 
CCGCCCAATG CCAATGGCGG TCAAACTCTC ATGATTACTC CAGCACCACA ACCAGCGCCT 
AAGGTTCCAA CTTTGGTGCG TCTCACCTTC CCCAACACGC CGATGGGTCA GGCACGGGCG 
CAGCTGCAGG AGCAAATCTT TAGCGCCATG GACATCTGCA CTCATCTTAC GGGCAAAGTG 
GTATCCCTTA CCCACTCGAA CGCCTACAAC CAGGTTCGGA GCTATCTGGA ACTGAAGGAG 
CTATACATTC ACATGTCCTA TTTGATGACC TATGCGATTG GGCGGTTTAA GAACCTGCAG 
GAGAAGTGTT TGGTGGACAT GCGAGAGAAG GGCTTCAAGA ATGACGCCAA CAGTCTGGAG 
AATGGTCAAC TAGCTGCTGA TAAACAAGCT AGTGATGACG AAGATAATGA AATCGAGATT 
GTGGAGCCCA AAACAGACAC CATTACCATT GATTCGGACA ACGAAGATGA CATGCCATCC 
ACCTCGACGG CAACACAACA AGAACAGCCT CAGGAAACGG CCATTATGAA GATTACATCC 
GTGACATCGG AGGCGTCGAA AAAGGAACTG CAAGAGAACA ACCAGGCCGA CATCGACGTG 
GCTGCATTCA GTTCCACGAT ATTGGCCAGC CTGCTGGAGG TGGAGGTCAA CGAAGGCGCC 
AGTGAGCCCA AGCCCGTATC TCCTGGCCAA AAGAAGCAGC CGCGCAAGAA AACTACAAAT 
AAGCCAAATC CAGCACTCTT GCAATTGGAA CGCCAACGCA TGGAGGACAT GAAAAAATCC 
GATGCCAAGC TGAAAATGAA GGTCATGGTC AAGCTGAAGC GAGCTGAGGA TGAGTTCGAG 
GTGGCGCGAA AATGGGTCGA GGACATGAAA CAGGGACAGG AATTGGCCAC CAAAATAAAG 
GACTCCAAAG CCGCAACACC ATCAAACAAA ACTCAAAAAC CAAGTGAAAA TCTGAAAATT 
TCTGAAAAAG GATCGTCAGC CAAAAAAGAG AGCACAAAGC AACACGGTGA GGGTGATAAG 
GAAAATACCA AGAATAAGGA AAAAATTGGA GATATATTGA AGGCGGCCAT AAAAGCCAAA 
ATTGGTGAAA ACGAGAAACT CATGGCTGAA GATGTACAGC TGCCAGTGGA TGAACTTCTA 
ACAGAAAAAG ATAAGGAATT GGACAAGGAA ACATTGACAT CCACTGAACC AGAAACAAAT 
AAAAAATCAG GAACAGAAAA AGGATCAGAC AAAAAACAAC ATTTAACAAA AGAAAACAAT 
GTAAAGGGAG AAAGTAAGGA TGAATCAAAA ACTGATCAGA AATCCGATTG TAAAAAAGGC 
CCGGATTCTG AATGTACAGA AATAGAAGAG GAGACTGAGA TGCTATCGGA AAAAGGATAT 
GGCGGGTCCG AAAAGGAACA AGGTGATACT GAAAATATGG AAGATGAAAC ATCTTCCGAA 
AGACCCACTT TAATTGGAGA AATCGAAAAA AATCTTCTCA AAGAGGGAAA AGAAGAGAGC 
TCGGAAAAAT TTGACAAGGA AGTGGAACCA TCAAAAGATG TTGGAAAAGA TTCCCCAACG 
ACCCAAGGTA AAGGTCAAGA TTCCGATAAA CTTAAGAACG CACGTGCGGG GCTAAAAAAT 
TCGACCCAAG ACGCTTCAAA CACAGTTGAA CCCCCCGTGG CCGCTTCCAA TGAAAATGAT 
CAGTCTTCCG TAGGGGAACT CATTGAAAGA ATGGACGTTA CCGAACTTGA TGAAAAGCCC 
AAAGAACTAC TAGATTGCGC CTTAGACGAA GTAATTCCCA TGGAGGGCAT CGAACTAAGT 
GATGATACAG AAGATCTGAT AAAAGCCTTG GGCAGCCCCA GCGTCCTGCC TTCCCAAAAT 
ACAAAGGATA ATGAGACTGA TTTGGATGCA AACGATTTTA TTGGAAATCT CCCACTCGAA 
ACAACGGAGC CACCTGAGAT TCTCTTGAAG GAGGATGAGT GCAAGGTGGA AAAGACAACA 
GGGATTTCAC AAGTAGGAGC ACCCAGCCCC GCCGAAAATG GTGACACAGA AATGGAATCC 
ACACTTGAAG CCAGTGACTC TGCGCCAGAC GAGTTCGCCA CGGCTGAGAT TGTTGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725094.1
CDS	297..3896
Translation	MSNSAPGSESGLAKVATPSPSPLAKLDADFTSSKFEMHPSLSDEERRFYLKMYPNVDLVNQRKVHCTVCKLHLGTAPAAESNIKMHPILRVTHCVKCHDFYNSGEFSKGEDGSELYCRWCGQGGEVYCCSTCPYVFCKSCIVKNLSKGVIVDIEQNENWNCFSCTSKILWPLRAQHWALVNYLQTQKRALQTLQLPEVEARRQMLKDNSNCCRLAKSKTSSLSDSMESLESNVSKRSQGSSAGSSKRGSMPKTIPPGPPSKRAKASNNEVVCTPDLLSMLEPDCQLSVTPKPTGRQTVTAANITTPRIVTVQQNYQPGQSSSNSGSNGSVSGSSSVPPTRSSLPPPLVLSGSGIRYRQNAPGGVSGVVRRTVVPNSVKTTGPVFHTINGFKVDLNSAAQQDSYRLPNGRLIQVKRQVPQTIQPASTVPTPPTPPALVVPQVGPGVVIRPRAPITTNRPSLHISTYGSNMGIYNPQTNQQQRAPQVRVGNANPAPPPLQGLPPNANGGQTLMITPAPQPAPKVPTLVRLTFPNTPMGQARAQLQEQIFSAMDICTHLTGKVVSLTHSNAYNQVRSYLELKELYIHMSYLMTYAIGRFKNLQEKCLVDMREKGFKNDANSLENGQLAADKQASDDEDNEIEIVEPKTDTITIDSDNEDDMPSTSTATQQEQPQETAIMKITSVTSEASKKELQENNQADIDVAAFSSTILASLLEVEVNEGASEPKPVSPGQKKQPRKKTTNKPNPALLQLERQRMEDMKKSDAKLKMKVMVKLKRAEDEFEVARKWVEDMKQGQELATKIKDSKAATPSNKTQKPSENLKISEKGSSAKKESTKQHGEGDKENTKNKEKIGDILKAAIKAKIGENEKLMAEDVQLPVDELLTEKDKELDKETLTSTEPETNKKSGTEKGSDKKQHLTKENNVKGESKDESKTDQKSDCKKGPDSECTEIEEETEMLSEKGYGGSEKEQGDTENMEDETSSERPTLIGEIEKNLLKEGKEESSEKFDKEVEPSKDVGKDSPTTQGKGQDSDKLKNARAGLKNSTQDASNTVEPPVAASNENDQSSVGELIERMDVTELDEKPKELLDCALDEVIPMEGIELSDDTEDLIKALGSPSVLPSQNTKDNETDLDANDFIGNLPLETTEPPEILLKEDECKVEKTTGISQVGAPSPAENGDTEMESTLEASDSAPDEFATAEIVG

Target ORF information:

RefSeq Version	NM_165856.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ADD domain-containing protein 1 (ADD1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165856.2

ORF Insert Sequence:

ATGAGTAACA GTGCTCCGGG TAGCGAAAGT GGCCTGGCAA AGGTAGCGAC GCCGTCGCCA 
TCGCCACTGG CCAAATTGGA TGCGGATTTC ACGAGCTCCA AGTTCGAGAT GCACCCCTCG 
CTTTCAGACG AGGAGCGCCG CTTCTACCTG AAGATGTACC CCAACGTAGA TCTGGTGAAC 
CAGCGAAAGG TGCACTGCAC CGTGTGCAAG CTTCACCTGG GCACAGCACC CGCCGCCGAG 
AGCAACATCA AGATGCATCC CATACTGCGG GTGACGCACT GCGTCAAGTG CCACGATTTT 
TACAACAGCG GCGAGTTCTC CAAGGGCGAG GATGGCTCTG AGCTGTACTG CCGCTGGTGC 
GGACAGGGCG GCGAGGTATA CTGCTGCTCC ACTTGCCCCT ACGTCTTCTG CAAGTCGTGC 
ATCGTGAAGA ACCTCTCCAA GGGCGTCATC GTGGACATCG AGCAAAACGA GAACTGGAAC 
TGCTTCAGCT GCACCTCCAA GATCCTGTGG CCACTACGAG CCCAACACTG GGCGCTGGTC 
AACTATCTTC AGACGCAGAA AAGGGCCCTA CAAACCCTTC AGCTTCCCGA GGTGGAGGCC 
CGACGTCAAA TGCTTAAGGA TAACAGCAAT TGCTGTCGCT TGGCGAAGTC GAAAACCAGC 
AGCTTATCCG ACTCGATGGA AAGCTTGGAG TCCAATGTCT CCAAACGCAG CCAGGGCAGC 
TCAGCGGGCT CGAGTAAACG GGGATCCATG CCAAAAACCA TTCCTCCGGG ACCACCATCC 
AAGCGCGCTA AAGCTTCCAA CAACGAAGTG GTCTGCACAC CGGATCTGCT CAGCATGCTG 
GAGCCGGATT GCCAGCTCTC TGTGACGCCC AAGCCTACTG GACGTCAGAC GGTGACAGCG 
GCAAATATCA CCACTCCGAG GATTGTCACC GTCCAACAGA ACTACCAACC GGGACAAAGT 
TCCAGCAACA GCGGCAGCAA CGGAAGCGTA AGTGGTTCTT CATCAGTGCC ACCGACACGT 
AGTAGCCTGC CACCTCCCTT GGTTCTCAGT GGATCTGGAA TCCGCTACCG CCAGAATGCC 
CCTGGTGGGG TCTCTGGTGT GGTACGTCGT ACTGTGGTTC CCAACTCTGT AAAAACCACT 
GGGCCAGTCT TTCACACAAT CAATGGGTTC AAAGTAGATT TAAATAGCGC TGCACAACAG 
GATTCCTATC GCCTGCCCAA TGGTAGACTA ATTCAGGTAA AACGTCAGGT ACCACAGACA 
ATTCAGCCGG CATCAACAGT ACCAACACCA CCAACACCGC CTGCTTTGGT CGTTCCTCAA 
GTGGGTCCGG GTGTGGTTAT CCGACCTCGA GCTCCAATTA CGACTAATAG ACCTTCACTG 
CATATCAGCA CGTACGGTTC TAACATGGGT ATCTATAACC CGCAGACGAA CCAACAGCAG 
CGGGCTCCGC AAGTGCGAGT TGGAAACGCC AATCCAGCGC CTCCACCGCT GCAAGGACTT 
CCGCCCAATG CCAATGGCGG TCAAACTCTC ATGATTACTC CAGCACCACA ACCAGCGCCT 
AAGGTTCCAA CTTTGGTGCG TCTCACCTTC CCCAACACGC CGATGGGTCA GGCACGGGCG 
CAGCTGCAGG AGCAAATCTT TAGCGCCATG GACATCTGCA CTCATCTTAC GGGCAAAGTG 
GTATCCCTTA CCCACTCGAA CGCCTACAAC CAGGTTCGGA GCTATCTGGA ACTGAAGGAG 
CTATACATTC ACATGTCCTA TTTGATGACC TATGCGATTG GGCGGTTTAA GAACCTGCAG 
GAGAAGTGTT TGGTGGACAT GCGAGAGAAG GGCTTCAAGA ATGACGCCAA CAGTCTGGAG 
AATGGTCAAC TAGCTGCTGA TAAACAAGCT AGTGATGACG AAGATAATGA AATCGAGATT 
GTGGAGCCCA AAACAGACAC CATTACCATT GATTCGGACA ACGAAGATGA CATGCCATCC 
ACCTCGACGG CAACACAACA AGAACAGCCT CAGGAAACGG CCATTATGAA GATTACATCC 
GTGACATCGG AGGCGTCGAA AAAGGAACTG CAAGAGAACA ACCAGGCCGA CATCGACGTG 
GCTGCATTCA GTTCCACGAT ATTGGCCAGC CTGCTGGAGG TGGAGGTCAA CGAAGGCGCC 
AGTGAGCCCA AGCCCGTATC TCCTGGCCAA AAGAAGCAGC CGCGCAAGAA AACTACAAAT 
AAGCCAAATC CAGCACTCTT GCAATTGGAA CGCCAACGCA TGGAGGACAT GAAAAAATCC 
GATGCCAAGC TGAAAATGAA GGTCATGGTC AAGCTGAAGC GAGCTGAGGA TGAGTTCGAG 
GTGGCGCGAA AATGGGTCGA GGACATGAAA CAGGGACAGG AATTGGCCAC CAAAATAAAG 
GACTCCAAAG CCGCAACACC ATCAAACAAA ACTCAAAAAC CAAGTGAAAA TCTGAAAATT 
TCTGAAAAAG GATCGTCAGC CAAAAAAGAG AGCACAAAGC AACACGGTGA GGGTGATAAG 
GAAAATACCA AGAATAAGGA AAAAATTGGA GATATATTGA AGGCGGCCAT AAAAGCCAAA 
ATTGGTGAAA ACGAGAAACT CATGGCTGAA GATGTACAGC TGCCAGTGGA TGAACTTCTA 
ACAGAAAAAG ATAAGGAATT GGACAAGGAA ACATTGACAT CCACTGAACC AGAAACAAAT 
AAAAAATCAG GAACAGAAAA AGGATCAGAC AAAAAACAAC ATTTAACAAA AGAAAACAAT 
GTAAAGGGAG AAAGTAAGGA TGAATCAAAA ACTGATCAGA AATCCGATTG TAAAAAAGGC 
CCGGATTCTG AATGTACAGA AATAGAAGAG GAGACTGAGA TGCTATCGGA AAAAGGATAT 
GGCGGGTCCG AAAAGGAACA AGGTGATACT GAAAATATGG AAGATGAAAC ATCTTCCGAA 
AGACCCACTT TAATTGGAGA AATCGAAAAA AATCTTCTCA AAGAGGGAAA AGAAGAGAGC 
TCGGAAAAAT TTGACAAGGA AGTGGAACCA TCAAAAGATG TTGGAAAAGA TTCCCCAACG 
ACCCAAGGTA AAGGTCAAGA TTCCGATAAA CTTAAGAACG CACGTGCGGG GCTAAAAAAT 
TCGACCCAAG ACGCTTCAAA CACAGTTGAA CCCCCCGTGG CCGCTTCCAA TGAAAATGAT 
CAGTCTTCCG TAGGGGAACT CATTGAAAGA ATGGACGTTA CCGAACTTGA TGAAAAGCCC 
AAAGAACTAC TAGATTGCGC CTTAGACGAA GTAATTCCCA TGGAGGGCAT CGAACTAAGT 
GATGATACAG AAGATCTGAT AAAAGCCTTG GGCAGCCCCA GCGTCCTGCC TTCCCAAAAT 
ACAAAGGATA ATGAGACTGA TTTGGATGCA AACGATTTTA TTGGAAATCT CCCACTCGAA 
ACAACGGAGC CACCTGAGAT TCTCTTGAAG GAGGATGAGT GCAAGGTGGA AAAGACAACA 
GGGATTTCAC AAGTAGGAGC ACCCAGCCCC GCCGAAAATG GTGACACAGA AATGGAATCC 
ACACTTGAAG CCAGTGACTC TGCGCCAGAC GAGTTCGCCA CGGCTGAGAT TGTTGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADD1 ( NM_136872.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610716.1 Drosophila melanogaster ADD domain-containing protein 1 (ADD1), transcript variant B, mRNA.

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CloneID	OFa09662
Clone ID Related Accession (Same CDS sequence)	NM_136872.3
Accession Version	NM_136872.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ADD domain-containing protein 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_136872.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTAACA GTGCTCCGGG TAGCGAAAGT GGCCTGGCAA AGGTAGCGAC GCCGTCGCCA 
TCGCCACTGG CCAAATTGGA TGCGGATTTC ACGAGCTCCA AGTTCGAGAT GCACCCCTCG 
CTTTCAGACG AGGAGCGCCG CTTCTACCTG AAGATGTACC CCAACGTAGA TCTGGTGAAC 
CAGCGAAAGG TGCACTGCAC CGTGTGCAAG CTTCACCTGG GCACAGCACC CGCCGCCGAG 
AGCAACATCA AGATGCATCC CATACTGCGG GTGACGCACT GCGTCAAGTG CCACGATTTT 
TACAACAGCG GCGAGTTCTC CAAGGGCGAG GATGGCTCTG AGCTGTACTG CCGCTGGTGC 
GGACAGGGCG GCGAGGTATA CTGCTGCTCC ACTTGCCCCT ACGTCTTCTG CAAGTCGTGC 
ATCGTGAAGA ACCTCTCCAA GGGCGTCATC GTGGACATCG AGCAAAACGA GAACTGGAAC 
TGCTTCAGCT GCACCTCCAA GATCCTGTGG CCACTACGAG CCCAACACTG GGCGCTGGTC 
AACTATCTTC AGACGCAGAA AAGGGCCCTA CAAACCCTTC AGCTTCCCGA GGTGGAGGCC 
CGACGTCAAA TGCTTAAGGA TAACAGCAAT TGCTGTCGCT TGGCGAAGTC GAAAACCAGC 
AGCTTATCCG ACTCGATGGA AAGCTTGGAG TCCAATGTCT CCAAACGCAG CCAGGGCAGC 
TCAGCGGGCT CGAGTAAACG GGGATCCATG CCAAAAACCA TTCCTCCGGG ACCACCATCC 
AAGCGCGCTA AAGCTTCCAA CAACGAAGTG GTCTGCACAC CGGATCTGCT CAGCATGCTG 
GAGCCGGATT GCCAGCTCTC TGTGACGCCC AAGCCTACTG GACGTCAGAC GGTGACAGCG 
GCAAATATCA CCACTCCGAG GATTGTCACC GTCCAACAGA ACTACCAACC GGGACAAAGT 
TCCAGCAACA GCGGCAGCAA CGGAAGCGTA AGTGGTTCTT CATCAGTGCC ACCGACACGT 
AGTAGCCTGC CACCTCCCTT GGTTCTCAGT GGATCTGGAA TCCGCTACCG CCAGAATGCC 
CCTGGTGGGG TCTCTGGTGT GGTACGTCGT ACTGTGGTTC CCAACTCTGT AAAAACCACT 
GGGCCAGTCT TTCACACAAT CAATGGGTTC AAAGTAGATT TAAATAGCGC TGCACAACAG 
GATTCCTATC GCCTGCCCAA TGGTAGACTA ATTCAGGTAA AACGTCAGGT ACCACAGACA 
ATTCAGCCGG CATCAACAGT ACCAACACCA CCAACACCGC CTGCTTTGGT CGTTCCTCAA 
GTGGGTCCGG GTGTGGTTAT CCGACCTCGA GCTCCAATTA CGACTAATAG ACCTTCACTG 
CATATCAGCA CGTACGGTTC TAACATGGGT ATCTATAACC CGCAGACGAA CCAACAGCAG 
CGGGCTCCGC AAGTGCGAGT TGGAAACGCC AATCCAGCGC CTCCACCGCT GCAAGGACTT 
CCGCCCAATG CCAATGGCGG TCAAACTCTC ATGATTACTC CAGCACCACA ACCAGCGCCT 
AAGGTTCCAA CTTTGGTGCG TCTCACCTTC CCCAACACGC CGATGGGTCA GGCACGGGCG 
CAGCTGCAGG AGCAAATCTT TAGCGCCATG GACATCTGCA CTCATCTTAC GGGCAAAGTG 
GTATCCCTTA CCCACTCGAA CGCCTACAAC CAGGTTCGGA GCTATCTGGA ACTGAAGGAG 
CTATACATTC ACATGTCCTA TTTGATGACC TATGCGATTG GGCGGTTTAA GAACCTGCAG 
GAGAAGTGTT TGGTGGACAT GCGAGAGAAG GGCTTCAAGA ATGACGCCAA CAGTCTGGAG 
AATGGTCAAC TAGCTGCTGC CGTGGATTAT GTGTGTTACG GCCAACTGGA CGACGTGGAT 
CTCTTCAACA CAGGCTGCAA TAGCTTTCAC AATCAGGTCT ATGAATACCG CAAGAGTTTG 
CATGCCAATC TTAAGGATGG CGAGGACTGC GAGCCATTGC CTCCGCTGAT GCCGCTGGGA 
GTTCGAGCCG AAGGTGATCC CATCGATGAG CCCGAGGAAC CGGAAACGGT ACCGGAACCG 
CAGCAGGATG ACACCGAAAA AGACCACGAA GATGCGGCCG AATTCGATTA TGATGAGCCA 
GAGGATTTGG ATGATGATTT GGATGATGAT GACTTGGACA ACGAGGAGTG TGGAGAACTG 
GGCACCGAAG AGAGCATGAA TCGGAATGAA CAAGCTCACG CCTACGATCG AAAGTTGACA 
CGCCTTCTCC GCGAGTATCC TTCGTTGTGG TGCACCCGTC ATCCCGATTA CGGCAAGAAA 
GAGGTCACTA ATAAGCAGTG GCGGGTGATT GCCAGTCATT TTCCACGCGG TGATGACATT 
AAGCTGCGCT GGAAGAACGT AAGGAAGCGA TACGTTCGGA TTGAGAGGCG CTTAAAGCAG 
GGAAAGCGTT TTAAAGGCTA CTTCGACAAA GCGACAAACT ATTTGGAGAA CAGGGATCGC 
CCGAGGAGCG AGTGGTTGGC GGTGGACTGT GGCGAAGATG ACGGCGATGG CTTTGAGCGC 
AAGCACCTCG ACGTAATAGA GAATAAGATG GAGCAAAAAG CTCATAAGCA GCCAGCAGTT 
AAAATCAACC TAAAAGGTGG ATCTAGAATA CCGACGCGAC CACTCGAGGT GCGTCCCATT 
GATCAACGCA TCATAACTTT CGCTAAAAGC CATCCAGTAT TATGGCGCAA ATCCGCTGAT 
CCCGAATTCA ACAGTATTGA CGAGCAAACT AGAAAGTCGC TGTGGCAAAA CTTTTGGAAG 
TCAGCGCCCA ATTATGCTTG CGAGTATCTT GTGGAGCGCT GGCAGCAGAT GTACGAGATG 
TACAAGTCCT TCCGCCTGAA GAGTATTAAA GATAAGGGAA TCTTTGCGAA TCTGGAGCTA 
AAGTACTCAA AGTACTTGGC CAGCTTGTAT TTCTTGTACA AGATCGATGA GCAGGACCTG 
CGCGATGAGT TTGAACCGCT GCAGGACTGT GTCCCGAGCG GCACTGACTC CGCTGGAAAC 
CTAAAAGATA TGCCCGATGA CAAGCAGTTC TGTCAGCAAC TGGTTTTGGC TATGCGTGCA 
TATCCAACAC TTTGGAATCC ACGACATGTT GACTACAGCG ATGTGGGAGC CAGGGAGCGC 
TTATGGCCGG AAGTGGCCAG GCGGTTACCC CGATTCAAGC GGGACGCTCA GGCCTGTAAG 
CTTCGCTGGC AGATGGCCAA GTTCGCTTAC GAGTGTTACC TACGCGAGAT GGAGCGCCAT 
CCGCGTGTCA ACGAACGAAA GTTGCAGAAG TTGCGCTCCA ACTTTCCGCT CGAGGAGATG 
CGCTTCCTCA ACATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610716.1
CDS	297..3674
Translation	MSNSAPGSESGLAKVATPSPSPLAKLDADFTSSKFEMHPSLSDEERRFYLKMYPNVDLVNQRKVHCTVCKLHLGTAPAAESNIKMHPILRVTHCVKCHDFYNSGEFSKGEDGSELYCRWCGQGGEVYCCSTCPYVFCKSCIVKNLSKGVIVDIEQNENWNCFSCTSKILWPLRAQHWALVNYLQTQKRALQTLQLPEVEARRQMLKDNSNCCRLAKSKTSSLSDSMESLESNVSKRSQGSSAGSSKRGSMPKTIPPGPPSKRAKASNNEVVCTPDLLSMLEPDCQLSVTPKPTGRQTVTAANITTPRIVTVQQNYQPGQSSSNSGSNGSVSGSSSVPPTRSSLPPPLVLSGSGIRYRQNAPGGVSGVVRRTVVPNSVKTTGPVFHTINGFKVDLNSAAQQDSYRLPNGRLIQVKRQVPQTIQPASTVPTPPTPPALVVPQVGPGVVIRPRAPITTNRPSLHISTYGSNMGIYNPQTNQQQRAPQVRVGNANPAPPPLQGLPPNANGGQTLMITPAPQPAPKVPTLVRLTFPNTPMGQARAQLQEQIFSAMDICTHLTGKVVSLTHSNAYNQVRSYLELKELYIHMSYLMTYAIGRFKNLQEKCLVDMREKGFKNDANSLENGQLAAAVDYVCYGQLDDVDLFNTGCNSFHNQVYEYRKSLHANLKDGEDCEPLPPLMPLGVRAEGDPIDEPEEPETVPEPQQDDTEKDHEDAAEFDYDEPEDLDDDLDDDDLDNEECGELGTEESMNRNEQAHAYDRKLTRLLREYPSLWCTRHPDYGKKEVTNKQWRVIASHFPRGDDIKLRWKNVRKRYVRIERRLKQGKRFKGYFDKATNYLENRDRPRSEWLAVDCGEDDGDGFERKHLDVIENKMEQKAHKQPAVKINLKGGSRIPTRPLEVRPIDQRIITFAKSHPVLWRKSADPEFNSIDEQTRKSLWQNFWKSAPNYACEYLVERWQQMYEMYKSFRLKSIKDKGIFANLELKYSKYLASLYFLYKIDEQDLRDEFEPLQDCVPSGTDSAGNLKDMPDDKQFCQQLVLAMRAYPTLWNPRHVDYSDVGARERLWPEVARRLPRFKRDAQACKLRWQMAKFAYECYLREMERHPRVNERKLQKLRSNFPLEEMRFLNI

Target ORF information:

RefSeq Version	NM_136872.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ADD domain-containing protein 1 (ADD1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136872.3

ORF Insert Sequence:

ATGAGTAACA GTGCTCCGGG TAGCGAAAGT GGCCTGGCAA AGGTAGCGAC GCCGTCGCCA 
TCGCCACTGG CCAAATTGGA TGCGGATTTC ACGAGCTCCA AGTTCGAGAT GCACCCCTCG 
CTTTCAGACG AGGAGCGCCG CTTCTACCTG AAGATGTACC CCAACGTAGA TCTGGTGAAC 
CAGCGAAAGG TGCACTGCAC CGTGTGCAAG CTTCACCTGG GCACAGCACC CGCCGCCGAG 
AGCAACATCA AGATGCATCC CATACTGCGG GTGACGCACT GCGTCAAGTG CCACGATTTT 
TACAACAGCG GCGAGTTCTC CAAGGGCGAG GATGGCTCTG AGCTGTACTG CCGCTGGTGC 
GGACAGGGCG GCGAGGTATA CTGCTGCTCC ACTTGCCCCT ACGTCTTCTG CAAGTCGTGC 
ATCGTGAAGA ACCTCTCCAA GGGCGTCATC GTGGACATCG AGCAAAACGA GAACTGGAAC 
TGCTTCAGCT GCACCTCCAA GATCCTGTGG CCACTACGAG CCCAACACTG GGCGCTGGTC 
AACTATCTTC AGACGCAGAA AAGGGCCCTA CAAACCCTTC AGCTTCCCGA GGTGGAGGCC 
CGACGTCAAA TGCTTAAGGA TAACAGCAAT TGCTGTCGCT TGGCGAAGTC GAAAACCAGC 
AGCTTATCCG ACTCGATGGA AAGCTTGGAG TCCAATGTCT CCAAACGCAG CCAGGGCAGC 
TCAGCGGGCT CGAGTAAACG GGGATCCATG CCAAAAACCA TTCCTCCGGG ACCACCATCC 
AAGCGCGCTA AAGCTTCCAA CAACGAAGTG GTCTGCACAC CGGATCTGCT CAGCATGCTG 
GAGCCGGATT GCCAGCTCTC TGTGACGCCC AAGCCTACTG GACGTCAGAC GGTGACAGCG 
GCAAATATCA CCACTCCGAG GATTGTCACC GTCCAACAGA ACTACCAACC GGGACAAAGT 
TCCAGCAACA GCGGCAGCAA CGGAAGCGTA AGTGGTTCTT CATCAGTGCC ACCGACACGT 
AGTAGCCTGC CACCTCCCTT GGTTCTCAGT GGATCTGGAA TCCGCTACCG CCAGAATGCC 
CCTGGTGGGG TCTCTGGTGT GGTACGTCGT ACTGTGGTTC CCAACTCTGT AAAAACCACT 
GGGCCAGTCT TTCACACAAT CAATGGGTTC AAAGTAGATT TAAATAGCGC TGCACAACAG 
GATTCCTATC GCCTGCCCAA TGGTAGACTA ATTCAGGTAA AACGTCAGGT ACCACAGACA 
ATTCAGCCGG CATCAACAGT ACCAACACCA CCAACACCGC CTGCTTTGGT CGTTCCTCAA 
GTGGGTCCGG GTGTGGTTAT CCGACCTCGA GCTCCAATTA CGACTAATAG ACCTTCACTG 
CATATCAGCA CGTACGGTTC TAACATGGGT ATCTATAACC CGCAGACGAA CCAACAGCAG 
CGGGCTCCGC AAGTGCGAGT TGGAAACGCC AATCCAGCGC CTCCACCGCT GCAAGGACTT 
CCGCCCAATG CCAATGGCGG TCAAACTCTC ATGATTACTC CAGCACCACA ACCAGCGCCT 
AAGGTTCCAA CTTTGGTGCG TCTCACCTTC CCCAACACGC CGATGGGTCA GGCACGGGCG 
CAGCTGCAGG AGCAAATCTT TAGCGCCATG GACATCTGCA CTCATCTTAC GGGCAAAGTG 
GTATCCCTTA CCCACTCGAA CGCCTACAAC CAGGTTCGGA GCTATCTGGA ACTGAAGGAG 
CTATACATTC ACATGTCCTA TTTGATGACC TATGCGATTG GGCGGTTTAA GAACCTGCAG 
GAGAAGTGTT TGGTGGACAT GCGAGAGAAG GGCTTCAAGA ATGACGCCAA CAGTCTGGAG 
AATGGTCAAC TAGCTGCTGC CGTGGATTAT GTGTGTTACG GCCAACTGGA CGACGTGGAT 
CTCTTCAACA CAGGCTGCAA TAGCTTTCAC AATCAGGTCT ATGAATACCG CAAGAGTTTG 
CATGCCAATC TTAAGGATGG CGAGGACTGC GAGCCATTGC CTCCGCTGAT GCCGCTGGGA 
GTTCGAGCCG AAGGTGATCC CATCGATGAG CCCGAGGAAC CGGAAACGGT ACCGGAACCG 
CAGCAGGATG ACACCGAAAA AGACCACGAA GATGCGGCCG AATTCGATTA TGATGAGCCA 
GAGGATTTGG ATGATGATTT GGATGATGAT GACTTGGACA ACGAGGAGTG TGGAGAACTG 
GGCACCGAAG AGAGCATGAA TCGGAATGAA CAAGCTCACG CCTACGATCG AAAGTTGACA 
CGCCTTCTCC GCGAGTATCC TTCGTTGTGG TGCACCCGTC ATCCCGATTA CGGCAAGAAA 
GAGGTCACTA ATAAGCAGTG GCGGGTGATT GCCAGTCATT TTCCACGCGG TGATGACATT 
AAGCTGCGCT GGAAGAACGT AAGGAAGCGA TACGTTCGGA TTGAGAGGCG CTTAAAGCAG 
GGAAAGCGTT TTAAAGGCTA CTTCGACAAA GCGACAAACT ATTTGGAGAA CAGGGATCGC 
CCGAGGAGCG AGTGGTTGGC GGTGGACTGT GGCGAAGATG ACGGCGATGG CTTTGAGCGC 
AAGCACCTCG ACGTAATAGA GAATAAGATG GAGCAAAAAG CTCATAAGCA GCCAGCAGTT 
AAAATCAACC TAAAAGGTGG ATCTAGAATA CCGACGCGAC CACTCGAGGT GCGTCCCATT 
GATCAACGCA TCATAACTTT CGCTAAAAGC CATCCAGTAT TATGGCGCAA ATCCGCTGAT 
CCCGAATTCA ACAGTATTGA CGAGCAAACT AGAAAGTCGC TGTGGCAAAA CTTTTGGAAG 
TCAGCGCCCA ATTATGCTTG CGAGTATCTT GTGGAGCGCT GGCAGCAGAT GTACGAGATG 
TACAAGTCCT TCCGCCTGAA GAGTATTAAA GATAAGGGAA TCTTTGCGAA TCTGGAGCTA 
AAGTACTCAA AGTACTTGGC CAGCTTGTAT TTCTTGTACA AGATCGATGA GCAGGACCTG 
CGCGATGAGT TTGAACCGCT GCAGGACTGT GTCCCGAGCG GCACTGACTC CGCTGGAAAC 
CTAAAAGATA TGCCCGATGA CAAGCAGTTC TGTCAGCAAC TGGTTTTGGC TATGCGTGCA 
TATCCAACAC TTTGGAATCC ACGACATGTT GACTACAGCG ATGTGGGAGC CAGGGAGCGC 
TTATGGCCGG AAGTGGCCAG GCGGTTACCC CGATTCAAGC GGGACGCTCA GGCCTGTAAG 
CTTCGCTGGC AGATGGCCAA GTTCGCTTAC GAGTGTTACC TACGCGAGAT GGAGCGCCAT 
CCGCGTGTCA ACGAACGAAA GTTGCAGAAG TTGCGCTCCA ACTTTCCGCT CGAGGAGATG 
CGCTTCCTCA ACATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ADD1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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