exp cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	exp
Entrez Gene ID	36276
Full Name	expansion
Synonyms	CG13188,Dmel\CG13188,Exp
General protein information	Preferred Names expansion Names CG13188 gene product from transcript CG13188-RA CG13188-PA CG13188-PB exp-PA exp-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 48D3-48D5

mRNA and Protein(s)

mRNA	Protein	Name
NM_136870.2	NP_610714.1	expansion, isoform B
NM_165853.2	NP_725091.1	expansion, isoform A

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007416	XP_308422.4
Drosophila melanogaster (fruit fly)	CG13188	NP_610714.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

To compare the Expansion and Smad MH2 domains, the crystal structure of the Expansion domain was determined at 1.6 A resolution, the first structure of a non-Smad MH2 domain to be characterized to date.
Title: Structure of the N-terminal domain of the protein Expansion: an 'Expansion' to the Smad MH2 fold.

This work provides a clear understanding of the role of Expansion, anovel Smad protein, in the regulation of Drosophila tracheal tube diameter.
Title: The novel Smad protein Expansion regulates the receptor tyrosine kinase pathway to control Drosophila tracheal tube size.

The following exp gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the exp cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa09658	NM_136870.2 Latest version!	Drosophila melanogaster expansion (exp), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa09659	NM_165853.2 Latest version!	Drosophila melanogaster expansion (exp), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

exp ( NM_136870.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610714.1 Drosophila melanogaster expansion (exp), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa09658
Clone ID Related Accession (Same CDS sequence)	NM_136870.2
Accession Version	NM_136870.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1542bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	expansion, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_136870.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGTCGC GTCGAAAAAT TCTATCACGT TCCCGCGACG ATCTTAATCT CGACCAGACG 
TTCACCCAGC AGGAGGAGGA GGAGGATATC TGGTTCCAGA AGGATAAACT ATACAAGGAG 
CACATTCAGG AAGTGCTGGA CAAATGGACC CAAATCGACG ACGAGATCTG GGCCAAAGTG 
ATCGTCTTCG AGCGGAATCG TCGCGTGGCC AAAGCGTACG CACGCGCCCC AGTGCTGACC 
ATTAATGGAT CCGACGATGG CTTCGACGGC ATGCGAATTG GACTGTGCGG CTTCGACAAT 
CCGATGAGGG ATCAGAAGAC CGACGAGATG AAGCGCGTCA TCGGACAGGG TGTTAAAATC 
AAAATGGATG ACGCTGGGAA CATCCTCATC CGGCGGTACG CCAAAAGCAA TGTCTACGTG 
AAGAGTACCG CCAGCAGTCC CAACGAGGAG ACCTCCATCG GTGCCGAGAT CTTAAAGCTG 
CCAAATCAGG CGCTCGAATC CGAAAAAATA GTCAAGCTCT TCGACATGAA GAAGTTCCAG 
TCGAATGTGA ATCGAGAACT GCGTCGCGCC TATCCGGATC GCCGCCGCTT GGAGACGCAG 
TGCCTCTCCG CGGTGGCGTT TGTCAAGTCG GAGAACGACA TCTTGGAGTG CCCCATCTGG 
GTGCTCATCG TTAATGTGGT GGCCATGGAT ATGCTGAAGA GCAAACTGCC ACCAGTGCAA 
CGTCCCATTG TGGACATCAA GAACCGCCCG CGCATTCCGA TTCCCGACGA GGATCCCTAC 
AGCGTCGCTG GCAACGGAAG TGGCGGTAGC TCCGGCAGCT CTGGCTTCGG CAGTGGCCAC 
CAGGCGCAAC TGCATCCGCA GCAACAGCTG GGCAAGCATC TCAATGGCAA CGGTGGAGGA 
CATGTGGCCG CCACGCGGGA GCAGCTGCTG CTGCAGACGC AACAACTGGC CCACAAGCGC 
AGCGAGAAAC CTCCGAAACT ACCGCCCAGG GATAATATCT ACTCGCACGA TCTGATTCCA 
AAGCCGGACT ACGATGATAT CGATTTGGAG ACCCGTATCA AACTGTCTCG CGGTAAATCT 
GACAAGGGCA AAGATAATAA GAAATATGAT GATCCCTACT ACTGCGGCCT GCGGGCCCGA 
GTTCCGAACT TTGTAAAGTC CGGCAAGCCG ATACCCGCTC AGGTTGCCAA GGATCAGCGG 
GATGGCAATG GGAACAATAT CGGGAACGGC GGCATCAGCA CGCTCAGCCA GAAGAAAACG 
TCGATGATCC ACAACCATTT GGGCGGCATG CATCCCCATC ACATTCAACA GCAGCAACTG 
ATGGCCGCGG CGGCGGCTGC GCATGCGGCA CAGCACCACC ATGCGGGTCA CCAGCGGGAG 
CAGCAGCAGC ACCACCAGAT TTGGCAGACT CGCAGCTATG AGAGCGGCAT AGATTCGGAG 
CTTGTTGAAT CACCCTACAA TCACATCTAC GGACGCCATT TACCGCTGCC AACGCGCGGC 
TACGTGCCCA CGCCACGCAT GTTCATCGGG GAATGGGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610714.1
CDS	707..2248
Translation	MVSRRKILSRSRDDLNLDQTFTQQEEEEDIWFQKDKLYKEHIQEVLDKWTQIDDEIWAKVIVFERNRRVAKAYARAPVLTINGSDDGFDGMRIGLCGFDNPMRDQKTDEMKRVIGQGVKIKMDDAGNILIRRYAKSNVYVKSTASSPNEETSIGAEILKLPNQALESEKIVKLFDMKKFQSNVNRELRRAYPDRRRLETQCLSAVAFVKSENDILECPIWVLIVNVVAMDMLKSKLPPVQRPIVDIKNRPRIPIPDEDPYSVAGNGSGGSSGSSGFGSGHQAQLHPQQQLGKHLNGNGGGHVAATREQLLLQTQQLAHKRSEKPPKLPPRDNIYSHDLIPKPDYDDIDLETRIKLSRGKSDKGKDNKKYDDPYYCGLRARVPNFVKSGKPIPAQVAKDQRDGNGNNIGNGGISTLSQKKTSMIHNHLGGMHPHHIQQQQLMAAAAAAHAAQHHHAGHQREQQQHHQIWQTRSYESGIDSELVESPYNHIYGRHLPLPTRGYVPTPRMFIGEWD

Target ORF information:

RefSeq Version	NM_136870.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster expansion (exp), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136870.2

ORF Insert Sequence:

ATGGTGTCGC GTCGAAAAAT TCTATCACGT TCCCGCGACG ATCTTAATCT CGACCAGACG 
TTCACCCAGC AGGAGGAGGA GGAGGATATC TGGTTCCAGA AGGATAAACT ATACAAGGAG 
CACATTCAGG AAGTGCTGGA CAAATGGACC CAAATCGACG ACGAGATCTG GGCCAAAGTG 
ATCGTCTTCG AGCGGAATCG TCGCGTGGCC AAAGCGTACG CACGCGCCCC AGTGCTGACC 
ATTAATGGAT CCGACGATGG CTTCGACGGC ATGCGAATTG GACTGTGCGG CTTCGACAAT 
CCGATGAGGG ATCAGAAGAC CGACGAGATG AAGCGCGTCA TCGGACAGGG TGTTAAAATC 
AAAATGGATG ACGCTGGGAA CATCCTCATC CGGCGGTACG CCAAAAGCAA TGTCTACGTG 
AAGAGTACCG CCAGCAGTCC CAACGAGGAG ACCTCCATCG GTGCCGAGAT CTTAAAGCTG 
CCAAATCAGG CGCTCGAATC CGAAAAAATA GTCAAGCTCT TCGACATGAA GAAGTTCCAG 
TCGAATGTGA ATCGAGAACT GCGTCGCGCC TATCCGGATC GCCGCCGCTT GGAGACGCAG 
TGCCTCTCCG CGGTGGCGTT TGTCAAGTCG GAGAACGACA TCTTGGAGTG CCCCATCTGG 
GTGCTCATCG TTAATGTGGT GGCCATGGAT ATGCTGAAGA GCAAACTGCC ACCAGTGCAA 
CGTCCCATTG TGGACATCAA GAACCGCCCG CGCATTCCGA TTCCCGACGA GGATCCCTAC 
AGCGTCGCTG GCAACGGAAG TGGCGGTAGC TCCGGCAGCT CTGGCTTCGG CAGTGGCCAC 
CAGGCGCAAC TGCATCCGCA GCAACAGCTG GGCAAGCATC TCAATGGCAA CGGTGGAGGA 
CATGTGGCCG CCACGCGGGA GCAGCTGCTG CTGCAGACGC AACAACTGGC CCACAAGCGC 
AGCGAGAAAC CTCCGAAACT ACCGCCCAGG GATAATATCT ACTCGCACGA TCTGATTCCA 
AAGCCGGACT ACGATGATAT CGATTTGGAG ACCCGTATCA AACTGTCTCG CGGTAAATCT 
GACAAGGGCA AAGATAATAA GAAATATGAT GATCCCTACT ACTGCGGCCT GCGGGCCCGA 
GTTCCGAACT TTGTAAAGTC CGGCAAGCCG ATACCCGCTC AGGTTGCCAA GGATCAGCGG 
GATGGCAATG GGAACAATAT CGGGAACGGC GGCATCAGCA CGCTCAGCCA GAAGAAAACG 
TCGATGATCC ACAACCATTT GGGCGGCATG CATCCCCATC ACATTCAACA GCAGCAACTG 
ATGGCCGCGG CGGCGGCTGC GCATGCGGCA CAGCACCACC ATGCGGGTCA CCAGCGGGAG 
CAGCAGCAGC ACCACCAGAT TTGGCAGACT CGCAGCTATG AGAGCGGCAT AGATTCGGAG 
CTTGTTGAAT CACCCTACAA TCACATCTAC GGACGCCATT TACCGCTGCC AACGCGCGGC 
TACGTGCCCA CGCCACGCAT GTTCATCGGG GAATGGGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

exp ( NM_165853.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725091.1 Drosophila melanogaster expansion (exp), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa09659
Clone ID Related Accession (Same CDS sequence)	NM_165853.2
Accession Version	NM_165853.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	expansion, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_165853.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGTCGC GTCGAAAAAT TCTATCACGT TCCCGCGACG ATCTTAATCT CGACCAGACG 
TTCACCCAGC AGGAGGAGGA GGAGGATATC TGGTTCCAGA AGGATAAACT ATACAAGGAG 
CACATTCAGG AAGTGCTGGA CAAATGGACC CAAATCGACG ACGAGATCTG GGCCAAAGTG 
ATCGTCTTCG AGCGGAATCG TCGCGTGGCC AAAGCGTACG CACGCGCCCC AGTGCTGACC 
ATTAATGGAT CCGACGATGG CTTCGACGGC ATGCGAATTG GACTGTGCGG CTTCGACAAT 
CCGATGAGGG ATCAGAAGAC CGACGAGATG AAGCGCGTCA TCGGACAGGG TGTTAAAATC 
AAAATGGATG ACGCTGGGAA CATCCTCATC CGGCGGTACG CCAAAAGCAA TGTCTACGTG 
AAGAGTACCG CCAGCAGTCC CAACGAGGAG ACCTCCATCG GTGCCGAGAT CTTAAAGCTG 
CCAAATCAGG CGCTCGAATC CGAAAAAATA GTCAAGCTCT TCGACATGAA GAAGTTCCAG 
TCGAATGTGA ATCGAGAACT GCGTCGCGCC TATCCGGATC GCCGCCGCTT GGAGACGCAG 
TGCCTCTCCG CGGTGGCGTT TGTCAAGTCG GAGAACGACA TCTTGGAGTG CCCCATCTGG 
GTGCTCATCG TTAATGTGGT GGCCATGGAT ATGCTGAAGA GCAAACTGCC ACCAGTGCAA 
CGTCCCATTG TGGACATCAA GAACCGCCCG CGCATTCCGA TTCCCGACGA GGATCCCTAC 
AGCGTCGCTG GCAACGGAAG TGGCGGTAGC TCCGGCAGCT CTGGCTTCGG CAGTGGCCAC 
CAGGCGCAAC TGCATCCGCA GCAACAGCTG GGCAAGCATC TCAATGGCAA CGGTGGAGGA 
CATGTGGCCG CCACGCGGGA GCAGCTGCTG CTGCAGACGC AACAACTGGC CCACAAGCGC 
AGCGAGAAAC CTCCGAAACT ACCGCCCAGG GATAATATCT ACTCGCACGA TCTGATTCCA 
AAGCCGGACT ACGATGATAT CGATTTGGAG ACCCGTATCA AACTGTCTCG CGGTAAATCT 
GACAAGGGCA AAGATAATAA GAAATATGAT GATCCCTACT ACTGCGGCCT GCGGGCCCGA 
GTTCCGAACT TTGTAAAGTC CGGCAAGCCG ATACCCGCTC AGGTTGCCAA GGATCAGCGG 
GATGGCAATG GGAACAATAT CGGGAACGGC GGCATCAGCA CGCTCAGCCA GAAGAAAACG 
TCGATGATCC ACAACCATTT GGGCGGCATG CATCCCCATC ACATTCAACA GCAGCAACTG 
ATGGCCGCGG CGGCGGCTGC GCATGCGGCA CAGCACCACC ATGCGGGTCA CCAGCGGGAG 
CAGCAGCAGC ACCACCAGAT TTGGCAGACT CGCAGCTATG AGAGCGGCAT AGGTATTTAT 
AAACAGGGTG GGCCCAACGC CGCCCACCAA TCACACAAAT CGCACGCACT GCAGATGCAA 
ATGCATCAGC AACTGCAAAT GCAAATGCAA CATCAACAAC AACAACAACC ACAGTCACTG 
CCAACGGACC TAACAGCAAC CACTGCAACA GCAACCACCA AGACACAAAG CACAGCTAAC 
CAAGCACGTA GCCACCAGCC ACATCCGCAT CTGCATCCGC ACTCGAATCC GCACGCACAT 
CCGCACCACA GACTCCACCG GCATGTCCAC GTCCGCTGCG AGCGGCACGC CCACTTGCAG 
CAGCAACACT CTGCACCGCT GCAATCAGTG CAGCACCAGC CGCAACAGCA GCAACAACCG 
TACTACGACA ACTTGGAGGA CTCTGTGGCT CGCCGGGTGA CACGGCGCCA CTCCACCCGT 
CGTTCTGCGC CGGAGGAGAG CTCCGCCTGC AATTGCGTGA GCTGCTATGC GCTGGCTCCA 
CTCTGTGGTG CAGTTCCTCC GCCACGTCCG CAAAGGAGCC TGAGTCAGCA GCAGCTGAGG 
CGTCACCAGC GGCTAAGTGG AAGCCTGGTG GAGCAGGAGG TCCGGCCGGC GGTGGTCAAG 
TGGTGCAGCG AGAGCGATGT CTCGGTGCTG GAGCTGGAAC CTACGGAACC CACAAACGAA 
CGCTACGACG ACTGCTACGA CTACGACGAC TTCGACATGG CATTCGACCT GGACGATGAC 
CTGGGCTTGA CCGAGAACAT ACCGCGCCGC AAGGGCACCG AAACCGTTGA CATGTACATG 
GATCGCTCCC ATCTCCGCCA GCTGCAGGCG CCGGCCGTGC TGCGCACCAA GTCACAGTCC 
ACCGAGAGCT TCTTCGAACA GCCCAACGAG GAGGGCTCAC TGTACATGTA CGGCGGCAGA 
AAGCGGATTG CGGTGAAGGC GCCGTCGGCG ACCAACATCT ATGACCGCGT GCGCGGCACC 
ATCGACTACT CGGACAGGAG ACGCAGTGCG GCAGTAGCAA AAGGGGGCCA GTGCCACAAG 
AAGCCACAGC TGCCAGTGCC CAGTTGGCGG GGCGGCAAGC GACAGGAGCA GGCTCAAAAC 
GCTGACAAAA GACAAAGATA TGACAATGCC ACCAATGGCA ACAGCCATGG CAACGGCAAC 
AACAGCAGCA ACAACCAGCA CAAAAGACAG CTAACGAACG CCAAGCAAGA CAATGAGGAC 
ATGTTGAAAA GCAAAAATTA TTTCAATAGT AAAAACAAAA GTATAGCAGC GGCAGCCGCA 
AATGCAGCAG CAGCAGCAGC CGCATCAGCA GCAACAACAG CAGCAGCGAA AGCGACAGCA 
GCATCAGCGA GCATTGCAAC AACATCTGGA AGCGGTGGAG CTGAGCCGGG AGCGGGAGCG 
GGCGCAACAG GAGTGGCTGG GGGAAAGGCC AGAGGAGGCG GTGGGCCAAC GATGGCCCCA 
CCACTGGCCA CCACTCCAGC CGCAAACAAT GCAACTGCGG TTAGTAAGGC ACCTTCTGCT 
CCTCCGCCGA CGGCCAGCAG AACCGGACGC AGTACCAGCC GCCTTGACAT CAGCGACATG 
GAATCCATTT ATGGGTACCT AAAGCCCCGC AAGCCGCGCA GCCTCAGCCT GAAGAAAATC 
CAAATACTCG ACTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725091.1
CDS	707..3784
Translation	MVSRRKILSRSRDDLNLDQTFTQQEEEEDIWFQKDKLYKEHIQEVLDKWTQIDDEIWAKVIVFERNRRVAKAYARAPVLTINGSDDGFDGMRIGLCGFDNPMRDQKTDEMKRVIGQGVKIKMDDAGNILIRRYAKSNVYVKSTASSPNEETSIGAEILKLPNQALESEKIVKLFDMKKFQSNVNRELRRAYPDRRRLETQCLSAVAFVKSENDILECPIWVLIVNVVAMDMLKSKLPPVQRPIVDIKNRPRIPIPDEDPYSVAGNGSGGSSGSSGFGSGHQAQLHPQQQLGKHLNGNGGGHVAATREQLLLQTQQLAHKRSEKPPKLPPRDNIYSHDLIPKPDYDDIDLETRIKLSRGKSDKGKDNKKYDDPYYCGLRARVPNFVKSGKPIPAQVAKDQRDGNGNNIGNGGISTLSQKKTSMIHNHLGGMHPHHIQQQQLMAAAAAAHAAQHHHAGHQREQQQHHQIWQTRSYESGIGIYKQGGPNAAHQSHKSHALQMQMHQQLQMQMQHQQQQQPQSLPTDLTATTATATTKTQSTANQARSHQPHPHLHPHSNPHAHPHHRLHRHVHVRCERHAHLQQQHSAPLQSVQHQPQQQQQPYYDNLEDSVARRVTRRHSTRRSAPEESSACNCVSCYALAPLCGAVPPPRPQRSLSQQQLRRHQRLSGSLVEQEVRPAVVKWCSESDVSVLELEPTEPTNERYDDCYDYDDFDMAFDLDDDLGLTENIPRRKGTETVDMYMDRSHLRQLQAPAVLRTKSQSTESFFEQPNEEGSLYMYGGRKRIAVKAPSATNIYDRVRGTIDYSDRRRSAAVAKGGQCHKKPQLPVPSWRGGKRQEQAQNADKRQRYDNATNGNSHGNGNNSSNNQHKRQLTNAKQDNEDMLKSKNYFNSKNKSIAAAAANAAAAAAASAATTAAAKATAASASIATTSGSGGAEPGAGAGATGVAGGKARGGGGPTMAPPLATTPAANNATAVSKAPSAPPPTASRTGRSTSRLDISDMESIYGYLKPRKPRSLSLKKIQILDY

Target ORF information:

RefSeq Version	NM_165853.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster expansion (exp), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165853.2

ORF Insert Sequence:

ATGGTGTCGC GTCGAAAAAT TCTATCACGT TCCCGCGACG ATCTTAATCT CGACCAGACG 
TTCACCCAGC AGGAGGAGGA GGAGGATATC TGGTTCCAGA AGGATAAACT ATACAAGGAG 
CACATTCAGG AAGTGCTGGA CAAATGGACC CAAATCGACG ACGAGATCTG GGCCAAAGTG 
ATCGTCTTCG AGCGGAATCG TCGCGTGGCC AAAGCGTACG CACGCGCCCC AGTGCTGACC 
ATTAATGGAT CCGACGATGG CTTCGACGGC ATGCGAATTG GACTGTGCGG CTTCGACAAT 
CCGATGAGGG ATCAGAAGAC CGACGAGATG AAGCGCGTCA TCGGACAGGG TGTTAAAATC 
AAAATGGATG ACGCTGGGAA CATCCTCATC CGGCGGTACG CCAAAAGCAA TGTCTACGTG 
AAGAGTACCG CCAGCAGTCC CAACGAGGAG ACCTCCATCG GTGCCGAGAT CTTAAAGCTG 
CCAAATCAGG CGCTCGAATC CGAAAAAATA GTCAAGCTCT TCGACATGAA GAAGTTCCAG 
TCGAATGTGA ATCGAGAACT GCGTCGCGCC TATCCGGATC GCCGCCGCTT GGAGACGCAG 
TGCCTCTCCG CGGTGGCGTT TGTCAAGTCG GAGAACGACA TCTTGGAGTG CCCCATCTGG 
GTGCTCATCG TTAATGTGGT GGCCATGGAT ATGCTGAAGA GCAAACTGCC ACCAGTGCAA 
CGTCCCATTG TGGACATCAA GAACCGCCCG CGCATTCCGA TTCCCGACGA GGATCCCTAC 
AGCGTCGCTG GCAACGGAAG TGGCGGTAGC TCCGGCAGCT CTGGCTTCGG CAGTGGCCAC 
CAGGCGCAAC TGCATCCGCA GCAACAGCTG GGCAAGCATC TCAATGGCAA CGGTGGAGGA 
CATGTGGCCG CCACGCGGGA GCAGCTGCTG CTGCAGACGC AACAACTGGC CCACAAGCGC 
AGCGAGAAAC CTCCGAAACT ACCGCCCAGG GATAATATCT ACTCGCACGA TCTGATTCCA 
AAGCCGGACT ACGATGATAT CGATTTGGAG ACCCGTATCA AACTGTCTCG CGGTAAATCT 
GACAAGGGCA AAGATAATAA GAAATATGAT GATCCCTACT ACTGCGGCCT GCGGGCCCGA 
GTTCCGAACT TTGTAAAGTC CGGCAAGCCG ATACCCGCTC AGGTTGCCAA GGATCAGCGG 
GATGGCAATG GGAACAATAT CGGGAACGGC GGCATCAGCA CGCTCAGCCA GAAGAAAACG 
TCGATGATCC ACAACCATTT GGGCGGCATG CATCCCCATC ACATTCAACA GCAGCAACTG 
ATGGCCGCGG CGGCGGCTGC GCATGCGGCA CAGCACCACC ATGCGGGTCA CCAGCGGGAG 
CAGCAGCAGC ACCACCAGAT TTGGCAGACT CGCAGCTATG AGAGCGGCAT AGGTATTTAT 
AAACAGGGTG GGCCCAACGC CGCCCACCAA TCACACAAAT CGCACGCACT GCAGATGCAA 
ATGCATCAGC AACTGCAAAT GCAAATGCAA CATCAACAAC AACAACAACC ACAGTCACTG 
CCAACGGACC TAACAGCAAC CACTGCAACA GCAACCACCA AGACACAAAG CACAGCTAAC 
CAAGCACGTA GCCACCAGCC ACATCCGCAT CTGCATCCGC ACTCGAATCC GCACGCACAT 
CCGCACCACA GACTCCACCG GCATGTCCAC GTCCGCTGCG AGCGGCACGC CCACTTGCAG 
CAGCAACACT CTGCACCGCT GCAATCAGTG CAGCACCAGC CGCAACAGCA GCAACAACCG 
TACTACGACA ACTTGGAGGA CTCTGTGGCT CGCCGGGTGA CACGGCGCCA CTCCACCCGT 
CGTTCTGCGC CGGAGGAGAG CTCCGCCTGC AATTGCGTGA GCTGCTATGC GCTGGCTCCA 
CTCTGTGGTG CAGTTCCTCC GCCACGTCCG CAAAGGAGCC TGAGTCAGCA GCAGCTGAGG 
CGTCACCAGC GGCTAAGTGG AAGCCTGGTG GAGCAGGAGG TCCGGCCGGC GGTGGTCAAG 
TGGTGCAGCG AGAGCGATGT CTCGGTGCTG GAGCTGGAAC CTACGGAACC CACAAACGAA 
CGCTACGACG ACTGCTACGA CTACGACGAC TTCGACATGG CATTCGACCT GGACGATGAC 
CTGGGCTTGA CCGAGAACAT ACCGCGCCGC AAGGGCACCG AAACCGTTGA CATGTACATG 
GATCGCTCCC ATCTCCGCCA GCTGCAGGCG CCGGCCGTGC TGCGCACCAA GTCACAGTCC 
ACCGAGAGCT TCTTCGAACA GCCCAACGAG GAGGGCTCAC TGTACATGTA CGGCGGCAGA 
AAGCGGATTG CGGTGAAGGC GCCGTCGGCG ACCAACATCT ATGACCGCGT GCGCGGCACC 
ATCGACTACT CGGACAGGAG ACGCAGTGCG GCAGTAGCAA AAGGGGGCCA GTGCCACAAG 
AAGCCACAGC TGCCAGTGCC CAGTTGGCGG GGCGGCAAGC GACAGGAGCA GGCTCAAAAC 
GCTGACAAAA GACAAAGATA TGACAATGCC ACCAATGGCA ACAGCCATGG CAACGGCAAC 
AACAGCAGCA ACAACCAGCA CAAAAGACAG CTAACGAACG CCAAGCAAGA CAATGAGGAC 
ATGTTGAAAA GCAAAAATTA TTTCAATAGT AAAAACAAAA GTATAGCAGC GGCAGCCGCA 
AATGCAGCAG CAGCAGCAGC CGCATCAGCA GCAACAACAG CAGCAGCGAA AGCGACAGCA 
GCATCAGCGA GCATTGCAAC AACATCTGGA AGCGGTGGAG CTGAGCCGGG AGCGGGAGCG 
GGCGCAACAG GAGTGGCTGG GGGAAAGGCC AGAGGAGGCG GTGGGCCAAC GATGGCCCCA 
CCACTGGCCA CCACTCCAGC CGCAAACAAT GCAACTGCGG TTAGTAAGGC ACCTTCTGCT 
CCTCCGCCGA CGGCCAGCAG AACCGGACGC AGTACCAGCC GCCTTGACAT CAGCGACATG 
GAATCCATTT ATGGGTACCT AAAGCCCCGC AAGCCGCGCA GCCTCAGCCT GAAGAAAATC 
CAAATACTCG ACTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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exp cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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