dap cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	dap
Entrez Gene ID	36001
Full Name	dacapo
Synonyms	CDI4,CDKN2B,CG1772,CIB1,Cdi4,DAP,Dac,Dap,Decapo,Dmel\CG1772,E(Sev-CycE)2B,P15,cdi4,dacapo/cyclin-dependent kinase interactor 4,l(2)04454,p21,p21[dacapo],p27,p27[Dap],p27cip/kip
General protein information	Preferred Names dacapo Names CG1772 gene product from transcript CG1772-RA CG1772-PA Cyclin-dependent kinase interactor 4 Dacap0 Dacapo/p21 chakra dacpao dap-PA decapo p21/Dacapo
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 46B1-46B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_057600.6	NP_476948.1	dacapo

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

in both Dicer-1 and bantam depleted genetic backgrounds, Dacapo, an inhibitor of cyclin E-Cdk complex, was found to have elevated expression.
Title: Dicer-1 regulates proliferative potential of Drosophila larval neural stem cells through bantam miRNA based down-regulation of the G1/S inhibitor Dacapo.

endo S phase-coupled destruction of Dap reduces the threshold of Cyclin E-Cdk2 activity necessary to trigger the subsequent G-S transition.
Title: Expression of an S phase-stabilized version of the CDK inhibitor Dacapo can alter endoreplication.

The Drosophila Skp2 regulates cell proliferation by targeting Dacapo for degradation.
Title: The Drosophila F-box protein dSkp2 regulates cell proliferation by targeting Dacapo for degradation.

PROSPERO provides a transient signal to ensure a precise timing for cell cycle exit of prospective neurons by activating the expression of DACAPO
Title: A transient expression of Prospero promotes cell cycle exit of Drosophila postembryonic neurons through the regulation of Dacapo.

Dcr-1 and Dap are required for nutrition-dependent cell cycle regulation in germline stem cells (GSCs) and reduction of dap partially rescues the cell cycle defect of Insulin receptor-deficient GSCs
Title: Dicer-1-dependent Dacapo suppression acts downstream of Insulin receptor in regulating cell division of Drosophila germline stem cells.

The following dap gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dap cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa12344	NM_057600.6 Latest version!	Drosophila melanogaster dacapo (dap), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dap ( NM_057600.6 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476948.1 Drosophila melanogaster dacapo (dap), mRNA.

Price & Availability↑

CloneID	OFa12344
Clone ID Related Accession (Same CDS sequence)	NM_057600.6
Accession Version	NM_057600.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 738bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dacapo
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_057600.5. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTCAGTG CCCGAGTCCT GAATCCTGTG ATGATCAGTG AGTTCTGCAA GATGAGCAGC 
AGCCCGGCGG TGAGTCGCAA TCTGGCATGC GGTCGTCAGT TGAATCGCAT AAAGCGTGAC 
CTCTTCGGTA GCTCCAAATC CGCCGAGGGT ACAGCCAACA AAACACCCTT TAATTCCGAA 
CTGGAGCGCC ATCAAGAACT GGCCACGCAA AAATGGGGCT TTGACTTCCG CGCCGGCTGC 
CCTTTAGCTG AAAAATCACC CTACATTTGG GAGCGCGTCA GCTTCCAGGA GTCGAGCTTC 
GCCCCAGAGA TGTACACCCT AACACGGGCC GCCCACGTAC GCCCTTCCGC GGATGCAAGT 
CCTAGCGACA TGGACATTTT GGTCAACGAG CGCTCAGAAC GGGAGAACTT TGGCTCCAAT 
CTGGTCAATT CCTCGCTAGA GTCGAACACG GATAATGAAT CTTGTTACGA CTCCCAGGAC 
GAGTCGCTGG CAATGCGGCT TAGCTCCTCC AGCACGACAA GCACATCCTC CATAGTCCTG 
CGCAAGAGAC AGCCAAAAAT CACAGAATTC ATGAAGGAGC GCAAACGTCT GGCCCAGGCA 
CCCAAGAAAC TATCGCCCGC AAAGCGCATG CGCACCAGTT CTCCCAGTTC GTCCGCCACT 
TCCAGCTCCA GCTCCAGCGG TGGATTTGGG TTGGGGGCAC ACTTTGGGGC GATGTTGAAG 
CGGCCGCGCC ACAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476948.1
CDS	620..1357
Translation	MVSARVLNPVMISEFCKMSSSPAVSRNLACGRQLNRIKRDLFGSSKSAEGTANKTPFNSELERHQELATQKWGFDFRAGCPLAEKSPYIWERVSFQESSFAPEMYTLTRAAHVRPSADASPSDMDILVNERSERENFGSNLVNSSLESNTDNESCYDSQDESLAMRLSSSSTTSTSSIVLRKRQPKITEFMKERKRLAQAPKKLSPAKRMRTSSPSSSATSSSSSSGGFGLGAHFGAMLKRPRHN

Target ORF information:

RefSeq Version	NM_057600.6
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dacapo (dap), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057600.6

ORF Insert Sequence:

ATGGTCAGTG CCCGAGTCCT GAATCCTGTG ATGATCAGTG AGTTCTGCAA GATGAGCAGC 
AGCCCGGCGG TGAGTCGCAA TCTGGCATGC GGTCGTCAGT TGAATCGCAT AAAGCGTGAC 
CTCTTCGGTA GCTCCAAATC CGCCGAGGGT ACAGCCAACA AAACACCCTT TAATTCCGAA 
CTGGAGCGCC ATCAAGAACT GGCCACGCAA AAATGGGGCT TTGACTTCCG CGCCGGCTGC 
CCTTTAGCTG AAAAATCACC CTACATTTGG GAGCGCGTCA GCTTCCAGGA GTCGAGCTTC 
GCCCCAGAGA TGTACACCCT AACACGGGCC GCCCACGTAC GCCCTTCCGC GGATGCAAGT 
CCTAGCGACA TGGACATTTT GGTCAACGAG CGCTCAGAAC GGGAGAACTT TGGCTCCAAT 
CTGGTCAATT CCTCGCTAGA GTCGAACACG GATAATGAAT CTTGTTACGA CTCCCAGGAC 
GAGTCGCTGG CAATGCGGCT TAGCTCCTCC AGCACGACAA GCACATCCTC CATAGTCCTG 
CGCAAGAGAC AGCCAAAAAT CACAGAATTC ATGAAGGAGC GCAAACGTCT GGCCCAGGCA 
CCCAAGAAAC TATCGCCCGC AAAGCGCATG CGCACCAGTT CTCCCAGTTC GTCCGCCACT 
TCCAGCTCCA GCTCCAGCGG TGGATTTGGG TTGGGGGCAC ACTTTGGGGC GATGTTGAAG 
CGGCCGCGCC ACAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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dap cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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