Cirl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cirl
Entrez Gene ID	35846
Full Name	Calcium-independent receptor for alpha-latrotoxin
Synonyms	BcDNA:GH07331,CG8639,CIRL,Dmel\CG8639,anon-WO0170980.7,anon-WO0170980.8,dCirl,dcirl
General protein information	Preferred Names Calcium-independent receptor for alpha-latrotoxin Names CG8639 gene product from transcript CG8639-RG CG8639-PB CG8639-PE CG8639-PF CG8639-PG CG8639-PH CG8639-PI CG8639-PJ CG8639-PK Cirl-PB Cirl-PE Cirl-PF Cirl-PG Cirl-PH Cirl-PI Cirl-PJ Cirl-PK latrophilin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 44D4-44D5

mRNA and Protein(s)

mRNA	Protein	Name
NM_136553.5	NP_610397.4	Calcium-independent receptor for alpha-latrotoxin, isoform G
NM_001144148.4	NP_001137620.1	Calcium-independent receptor for alpha-latrotoxin, isoform B
NM_001169619.4	NP_001163090.1	Calcium-independent receptor for alpha-latrotoxin, isoform E
NM_001273880.2	NP_001260809.1	Calcium-independent receptor for alpha-latrotoxin, isoform K
NM_001273879.2	NP_001260808.1	Calcium-independent receptor for alpha-latrotoxin, isoform J
NM_001259276.2	NP_001246205.1	Calcium-independent receptor for alpha-latrotoxin, isoform H
NM_001273878.1	NP_001260807.1	Calcium-independent receptor for alpha-latrotoxin, isoform I
NM_001259275.2	NP_001246204.1	Calcium-independent receptor for alpha-latrotoxin, isoform F

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

dCirl shapes the perception of tactile, proprioceptive, and auditory stimuli through chordotonal neurons, the principal mechanosensors of Drosophila.
Title: The adhesion GPCR latrophilin/CIRL shapes mechanosensation.

The following Cirl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cirl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa09296	NM_001259276.2 Latest version!	Drosophila melanogaster Calcium-independent receptor for alpha-latrotoxin (Cirl), transcript variant H, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OFa09296	NM_001144148.4 Latest version!	Drosophila melanogaster Calcium-independent receptor for alpha-latrotoxin (Cirl), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OFa09297	NM_001273878.1 Latest version!	Drosophila melanogaster Calcium-independent receptor for alpha-latrotoxin (Cirl), transcript variant I, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OFa09298	NM_001273880.2 Latest version!	Drosophila melanogaster Calcium-independent receptor for alpha-latrotoxin (Cirl), transcript variant K, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa09298	NM_001259275.2 Latest version!	Drosophila melanogaster Calcium-independent receptor for alpha-latrotoxin (Cirl), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa09299	NM_001273879.2 Latest version!	Drosophila melanogaster Calcium-independent receptor for alpha-latrotoxin (Cirl), transcript variant J, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa09300	NM_001169619.4 Latest version!	Drosophila melanogaster Calcium-independent receptor for alpha-latrotoxin (Cirl), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OFa12785	NM_136553.5 Latest version!	Drosophila melanogaster Calcium-independent receptor for alpha-latrotoxin (Cirl), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cirl ( NM_001144148.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137620.1 Drosophila melanogaster Calcium-independent receptor for alpha-latrotoxin (Cirl), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa09296
Clone ID Related Accession (Same CDS sequence)	NM_001144148.4 , NM_001259276.2
Accession Version	NM_001144148.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Calcium-independent receptor for alpha-latrotoxin, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001144148.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTCCCA AGTCACTCAG CGTACTGAAC TCAAGATGTG CCCACAAGCA GAGCTGCGGC 
GTGTTGGCAG CCACGAGCAT GTTCGGGGAT CCCTGTCCCG GTACCCACAA GTATCTGGAG 
GCACACTACC AGTGCATAAG TGCAGCCCAA ACTTCGACGA CGACCAACAG GCCCAGTCCG 
CCGCCATGGG TGCTGAGCAA TGGTCCGCCG ATCTTTGGCA ACGGCAGTGG ACTGATCCAT 
CCGCCCGGGG TTGGAGCGGG TGCGCCGCCC CCGCCGAGAC TTCCCACACT TCCCGGAGTG 
GTGGGAATCA GTGGGAATCC CGGCCTGTTC AACGTACCAC CGCAACACAC CGCCGTCACG 
CACTCCACGC CCTCGAGCAG CACGACAGCC GTGGGCGGTG GACGTTTGAA GGGTGGGGCC 
ACCTCCACGA CGACCACCAA GCATCCGGCT GGCCGCCATG ATGGTCTGCC ACCGCCGCCG 
CAACTGCACC ACCACCACAA CCACCACGGT GAAGACACTG CCTCACCCAC CAAGCCGAGC 
AGCAAGCTGC CGGCTGGCGG TAATGCCACT TCACCATCCA ACACGAGGAT ACTCACGGGC 
GTCGGAGGTT CCGGAACTGA TGACGGAACC CTACTGACCA CAAAGAGCTC ACCCAACCGC 
CCACCGGGCA CTGCGGCCAG TGGATCCGTT GTCCCCGGGA ACGGCAGCGT GGTGCGCACC 
ATCAACAATA TTAATTTGAA CGCAGCCGGG ATGTCCGGAG GCGATGATGA GTCCAAGTTG 
TTTTGCGGCC CCACTCATGC CCGCAATTTG TACTGGAACA TGACTCGAGT GGGTGATGTG 
AATGTTCAGC CCTGTCCTGG CGGAGCAGCC GGCATCGCCA AGTGGCGTTG CGTTCTAATG 
AAGAGGATAC CCGACTCCGG CTACGATGAG TACGATGATG ACATCAGTTC GACAACTCCG 
GCACCCAGCG GTGGCGACTG TCTGCACAAC AGCAGCAGCT GCGAGCCGCC GGTGAGCATG 
GCCCACAAGG TAAACCAGCG TCTGCGCAAC TTTGAGCCCA CCTGGCATCC CGCGACACCT 
GATCTGACGC AATGCCGCAG CCTTTGGCTC AACAATCTGG AAATGCGAGT AAACCAGCGG 
GACTCCTCCT TGATCTCCAT CGCCAACGAT ATGTCCGAAG TGACCAGTAG CAAAACGCTC 
TACGGCGGCG ACATGTTGGT CACCACGAAG ATTATCCAAA CAGTGTCCGA GAAGATGATG 
CACGACAAGG AGACCTTCCC GGATCAGCGA CAGCGCGAGG CTATGATCAT GGAGTTGTTG 
CATTGTGTGG TCAAAACCGG CTCCAACCTG CTGGACGAAT CGCAGCTGTC CTCGTGGTTG 
GATCTCAATC CGGAGGACCA AATGCGTGTA GCCACATCCT TGCTAACTGG CCTGGAATAC 
AATGCCTTTC TGCTGGCGGA TACGATCATC AGGGAGCGCA GCGTGGTGCA AAAAGTCAAA 
AATATATTGC TCTCCGTTCG AGTTCTGGAA ACCAAGACTA TCCAGTCCAG CGTGGTCTTC 
CCAGATTCGG ATCAGTGGCC CTTGAGTTCG GATCGTATTG AGCTGCCACG AGCTGCTCTA 
ATAGATAATA GTGAAGGCGG TCTGGTGCGA ATTGTATTCG CCGCCTTCGA TCGCCTGGAA 
TCCATTCTAA AGCCCAGCTA TGATCACTTC GATCTCAAGA GCTCCCGCAG TTACGTTCGC 
AATACAGCCA TCCTGAGCAA CGACAGCGAT GTCAACGCGG GGGAGATCCA ACAGCGCCTA 
CGCATCCTGA ACAGCAAGGT GATCTCGGCC AGCTTGGGCA AGGGGCGTCA CATACAACTC 
TCCCAGCCCA TAACCCTGAC ACTGAAACAT CTGAAGACCG AGAATGTAAC GAATCCCACC 
TGCGTGTTCT GGAACTATAT TGACCATGCG TGGTCTGCCA ACGGATGCAG TCTGGAGTCC 
ACTAACCGCA CGCACAGCGT CTGCAGTTGC AACCACCTGA CAAACTTTGC CATACTAATG 
GACGTTGTGG ATGAGCACCA GCATTCGTTG TTCACCATGT TCGATGGAAA CATGCGCATA 
TTCATCTACA TAAGCATCGG CATCTGCGTG GTCTTCATAG TTATCGCCCT GCTAACGCTG 
AAGCTGTTCA ATGGGGTCTT TGTGAAGTCC GCGCGCACCT CGATCTATAC CAGCATTTAC 
CTTTGCCTCC TGGCCATCGA GCTGCTCTTT CTCCTGGGCA TTGAACAGAC CGAAACAAGC 
ATTTTCTGCG GCTTCATTAC TATTTTCCTA CACTGTGCCA TCCTATCGGG CACCGCCTGG 
TTCTGTTACG AAGCCTTCCA TTCGTACTCA ACGCTCACCT CGGACGAGCT CCTGCTGGAG 
GTGGACCAGA CGCCCAAGGT GAACTGCTAC TACCTCTTGT CCTACGGACT GTCGCTGAGC 
GTGGTGGCCA TCTCGCTGGT CATCGATCCC AGCACCTATA CCCAAAACGA TTATTGCGTG 
CTGATGGAGG CGAATGCCTT GTTTTATGCC ACCTTTGTAA TACCAGTGCT TGTCTTCTTT 
GTGGCTGCCA TTGGTTACAC ATTCCTCTCC TGGATTATAA TGTGCCGCAA AAGTCGCACG 
GGTCTAAAGA CCAAGGAACA TACTCGCCTC GCTAGCGTGC GGTTCGACAT ACGCTGCTCC 
TTTGTGTTCC TCTTGCTGCT CAGCGCTGTT TGGTGCTCGG CCTACTTCTA TTTGCGAGGA 
GCCAAAATGG ACGATGACAC GGCTGATGTG TATGGATACT GCTTCATCTG CTTCAACACA 
TTGCTGGGGC TCTATATCTT CGTGTTCCAT TGCATTCAAA ACGAAAAGAT CCGGCGGGAG 
TATCGGAAGT ATGTGAGACA GCACGCTTGG CTGCCCAAGT GCTTGCGCTG CTCGAAAACA 
TCAATTTCCT CGGGCATTGT TACCGGCAAT GGACCCACAG CCGGAACCCT TTGCAGCGTC 
TCCACGTCCA AGAAGCCCAA GCTGCCGTTA GGAGTGAGCG AAGAGGCGCA TGACGATCCC 
CAGCAGCAAC AGCAGACACC AGTGCCCATC ACAGAGGATG CCATTATGGG AGCCACCTCT 
GATTGTGAAC TGAACGAGGC CCAGCAAAGA AGAACCCTAA AAAGTGGCCT AATGACGGGC 
ACACTACAGG CTCCACCGCA GACCCTTGGT GGCCATGTTG TGCTCGAAAG AGGTAGCACT 
CTCCGCTCCA CTGGTCATGC CTCACCCACC AGCTCTGCCG GGTCCACACA CCTGATTTTT 
GCGCACAAGC AACAACAACA ACAGCAGCAA CAGGGACCTT TGGGCGAGTC TTACTACCAT 
CAGCCGGACT ACTACAGCTG GAAGCAACCA TCAACTGGAA CAGGAGGATT GAAAACACCG 
CGGGAGTACT ACAATAATGC GGGTGCTGCT GCATCATCGC CGCAACAGGC GCACGAGGTA 
TTCTACTGGA CTCAAAAGCC GAACAGCGGC CACAATGGCA AAAAGAAGAG GGGCGCCGGA 
GGAGTTCCCG CCTCGCCTAG TGGATCTCTG CACAGTCGCA CGGCCGCCGC CTCCCAGGTG 
CTTTTCTATC CATCGTACAA GAAGACCAAG CCTGGCCAGC CAACAGGCTA TCCGCAATAC 
GCGGAGGCGT TGGACCCACC ACTAGCCACT GGCAATGCGG CTGCCTACTA CCAGCAGCAG 
CAACAGTTGC GTCGCCAGCA GCTACATCAG CAGCAGCAAC AGCAGCAGCA GCAGCAACTC 
TCCTCGGACG AGGAGCAGGC CGAGCAACAT GCTCACCTGT TGCACCTGCA ACGACGAGCT 
GGTAGCCAGC AGCAGCTCCC TGCTCCACCG CCACACATGG CGCAGTACCA GCAGGAGTTT 
ATGCAGCGCC AGTATAGAAA TAAGCATTCC AACTGTGATC TGGGCATGGG CGATGCCTAC 
TACAACCAAG GCAGCGTCGG CGGCGCGGAT GGTGGGCCGG TCTACGAGGA GATCCTCAGC 
AACCGCAACT CGGATGTGCA GCATTACGAG GTGGGTGACT TCGATGTGGA CGAGGTGTAC 
AACAATAGCG TTGGCACTGG CGTCTTCAAC AACATGAGAG CGGCGGTGGC CGCCGGCGGT 
AGTCGGTACG GCGGCGGAAG CCTGAGTGGC GGCAGTGTCT CGTCCAGGAG CCAACAGCAG 
CAGCTCAAGA AGCAGCAGCA ACAACAGTCG CTGGCTCAGC AAAGATCGGC TCGACGCTGC 
ACGGCGGATG ACGATGACGA CGAAGACGAG GAGGAGGATG AGGAGGCAAC GGCCGCGGAG 
CAATTGCACG ACAGCGTCTG TGATGAGGAT GAGGAGGAGG ACGAGAGCGA CTTGGAGGAT 
GATGCTCATG GATTGCCACC GCAGAGCGAT GAGCGGATGC GTCGTCTGAT GGCGATGCAG 
GACGAGGATT TTAAGCGGCG GTTTCAACGT CAGCTGCGCA AACATGGAGC GCCTCTTGAC 
TACGGGGCTT TGCCACCAGG AGCAGGACCG CAACCCGAGC ACAACGGTGC GGTTTTTGGG 
GTTAGCGGCG GCGTTGGTGA GGGCTCCCTG CGTGGCGCAT TCCGGCAGCA GCAGCAGCAA 
GCACTGAATG CCAAGTCGCC AGGCGGCCGT TTGGCGGTAA ATGAGCTATT CGGTCACGGC 
AACTCGGGAC CACCGCTGCC GCCGGCGAAT CAGACGCCCG CGCAGAAGCG CCAGCAGCTA 
CAGAAACTGT CACCACAGTC CACCACATCA TCGTCGTCGC ATACATCACA CAGCAATCCC 
AATCCGCATC CCCACCAGCT AACCCATCCG CATCCCCATC AGCATCCTCC GCACCATCAG 
CAGCGCCACC TGTCGGCCAT GCTCGATGAG AACAACACGG TGCGATGTTA TCTGGAACCA 
CTGGCTAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137620.1
CDS	313..5244
Translation	MFPKSLSVLNSRCAHKQSCGVLAATSMFGDPCPGTHKYLEAHYQCISAAQTSTTTNRPSPPPWVLSNGPPIFGNGSGLIHPPGVGAGAPPPPRLPTLPGVVGISGNPGLFNVPPQHTAVTHSTPSSSTTAVGGGRLKGGATSTTTTKHPAGRHDGLPPPPQLHHHHNHHGEDTASPTKPSSKLPAGGNATSPSNTRILTGVGGSGTDDGTLLTTKSSPNRPPGTAASGSVVPGNGSVVRTINNINLNAAGMSGGDDESKLFCGPTHARNLYWNMTRVGDVNVQPCPGGAAGIAKWRCVLMKRIPDSGYDEYDDDISSTTPAPSGGDCLHNSSSCEPPVSMAHKVNQRLRNFEPTWHPATPDLTQCRSLWLNNLEMRVNQRDSSLISIANDMSEVTSSKTLYGGDMLVTTKIIQTVSEKMMHDKETFPDQRQREAMIMELLHCVVKTGSNLLDESQLSSWLDLNPEDQMRVATSLLTGLEYNAFLLADTIIRERSVVQKVKNILLSVRVLETKTIQSSVVFPDSDQWPLSSDRIELPRAALIDNSEGGLVRIVFAAFDRLESILKPSYDHFDLKSSRSYVRNTAILSNDSDVNAGEIQQRLRILNSKVISASLGKGRHIQLSQPITLTLKHLKTENVTNPTCVFWNYIDHAWSANGCSLESTNRTHSVCSCNHLTNFAILMDVVDEHQHSLFTMFDGNMRIFIYISIGICVVFIVIALLTLKLFNGVFVKSARTSIYTSIYLCLLAIELLFLLGIEQTETSIFCGFITIFLHCAILSGTAWFCYEAFHSYSTLTSDELLLEVDQTPKVNCYYLLSYGLSLSVVAISLVIDPSTYTQNDYCVLMEANALFYATFVIPVLVFFVAAIGYTFLSWIIMCRKSRTGLKTKEHTRLASVRFDIRCSFVFLLLLSAVWCSAYFYLRGAKMDDDTADVYGYCFICFNTLLGLYIFVFHCIQNEKIRREYRKYVRQHAWLPKCLRCSKTSISSGIVTGNGPTAGTLCSVSTSKKPKLPLGVSEEAHDDPQQQQQTPVPITEDAIMGATSDCELNEAQQRRTLKSGLMTGTLQAPPQTLGGHVVLERGSTLRSTGHASPTSSAGSTHLIFAHKQQQQQQQQGPLGESYYHQPDYYSWKQPSTGTGGLKTPREYYNNAGAAASSPQQAHEVFYWTQKPNSGHNGKKKRGAGGVPASPSGSLHSRTAAASQVLFYPSYKKTKPGQPTGYPQYAEALDPPLATGNAAAYYQQQQQLRRQQLHQQQQQQQQQQLSSDEEQAEQHAHLLHLQRRAGSQQQLPAPPPHMAQYQQEFMQRQYRNKHSNCDLGMGDAYYNQGSVGGADGGPVYEEILSNRNSDVQHYEVGDFDVDEVYNNSVGTGVFNNMRAAVAAGGSRYGGGSLSGGSVSSRSQQQQLKKQQQQQSLAQQRSARRCTADDDDDEDEEEDEEATAAEQLHDSVCDEDEEEDESDLEDDAHGLPPQSDERMRRLMAMQDEDFKRRFQRQLRKHGAPLDYGALPPGAGPQPEHNGAVFGVSGGVGEGSLRGAFRQQQQQALNAKSPGGRLAVNELFGHGNSGPPLPPANQTPAQKRQQLQKLSPQSTTSSSSHTSHSNPNPHPHQLTHPHPHQHPPHHQQRHLSAMLDENNTVRCYLEPLAK

Target ORF information:

RefSeq Version	NM_001144148.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Calcium-independent receptor for alpha-latrotoxin (Cirl), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144148.4

ORF Insert Sequence:

ATGTTTCCCA AGTCACTCAG CGTACTGAAC TCAAGATGTG CCCACAAGCA GAGCTGCGGC 
GTGTTGGCAG CCACGAGCAT GTTCGGGGAT CCCTGTCCCG GTACCCACAA GTATCTGGAG 
GCACACTACC AGTGCATAAG TGCAGCCCAA ACTTCGACGA CGACCAACAG GCCCAGTCCG 
CCGCCATGGG TGCTGAGCAA TGGTCCGCCG ATCTTTGGCA ACGGCAGTGG ACTGATCCAT 
CCGCCCGGGG TTGGAGCGGG TGCGCCGCCC CCGCCGAGAC TTCCCACACT TCCCGGAGTG 
GTGGGAATCA GTGGGAATCC CGGCCTGTTC AACGTACCAC CGCAACACAC CGCCGTCACG 
CACTCCACGC CCTCGAGCAG CACGACAGCC GTGGGCGGTG GACGTTTGAA GGGTGGGGCC 
ACCTCCACGA CGACCACCAA GCATCCGGCT GGCCGCCATG ATGGTCTGCC ACCGCCGCCG 
CAACTGCACC ACCACCACAA CCACCACGGT GAAGACACTG CCTCACCCAC CAAGCCGAGC 
AGCAAGCTGC CGGCTGGCGG TAATGCCACT TCACCATCCA ACACGAGGAT ACTCACGGGC 
GTCGGAGGTT CCGGAACTGA TGACGGAACC CTACTGACCA CAAAGAGCTC ACCCAACCGC 
CCACCGGGCA CTGCGGCCAG TGGATCCGTT GTCCCCGGGA ACGGCAGCGT GGTGCGCACC 
ATCAACAATA TTAATTTGAA CGCAGCCGGG ATGTCCGGAG GCGATGATGA GTCCAAGTTG 
TTTTGCGGCC CCACTCATGC CCGCAATTTG TACTGGAACA TGACTCGAGT GGGTGATGTG 
AATGTTCAGC CCTGTCCTGG CGGAGCAGCC GGCATCGCCA AGTGGCGTTG CGTTCTAATG 
AAGAGGATAC CCGACTCCGG CTACGATGAG TACGATGATG ACATCAGTTC GACAACTCCG 
GCACCCAGCG GTGGCGACTG TCTGCACAAC AGCAGCAGCT GCGAGCCGCC GGTGAGCATG 
GCCCACAAGG TAAACCAGCG TCTGCGCAAC TTTGAGCCCA CCTGGCATCC CGCGACACCT 
GATCTGACGC AATGCCGCAG CCTTTGGCTC AACAATCTGG AAATGCGAGT AAACCAGCGG 
GACTCCTCCT TGATCTCCAT CGCCAACGAT ATGTCCGAAG TGACCAGTAG CAAAACGCTC 
TACGGCGGCG ACATGTTGGT CACCACGAAG ATTATCCAAA CAGTGTCCGA GAAGATGATG 
CACGACAAGG AGACCTTCCC GGATCAGCGA CAGCGCGAGG CTATGATCAT GGAGTTGTTG 
CATTGTGTGG TCAAAACCGG CTCCAACCTG CTGGACGAAT CGCAGCTGTC CTCGTGGTTG 
GATCTCAATC CGGAGGACCA AATGCGTGTA GCCACATCCT TGCTAACTGG CCTGGAATAC 
AATGCCTTTC TGCTGGCGGA TACGATCATC AGGGAGCGCA GCGTGGTGCA AAAAGTCAAA 
AATATATTGC TCTCCGTTCG AGTTCTGGAA ACCAAGACTA TCCAGTCCAG CGTGGTCTTC 
CCAGATTCGG ATCAGTGGCC CTTGAGTTCG GATCGTATTG AGCTGCCACG AGCTGCTCTA 
ATAGATAATA GTGAAGGCGG TCTGGTGCGA ATTGTATTCG CCGCCTTCGA TCGCCTGGAA 
TCCATTCTAA AGCCCAGCTA TGATCACTTC GATCTCAAGA GCTCCCGCAG TTACGTTCGC 
AATACAGCCA TCCTGAGCAA CGACAGCGAT GTCAACGCGG GGGAGATCCA ACAGCGCCTA 
CGCATCCTGA ACAGCAAGGT GATCTCGGCC AGCTTGGGCA AGGGGCGTCA CATACAACTC 
TCCCAGCCCA TAACCCTGAC ACTGAAACAT CTGAAGACCG AGAATGTAAC GAATCCCACC 
TGCGTGTTCT GGAACTATAT TGACCATGCG TGGTCTGCCA ACGGATGCAG TCTGGAGTCC 
ACTAACCGCA CGCACAGCGT CTGCAGTTGC AACCACCTGA CAAACTTTGC CATACTAATG 
GACGTTGTGG ATGAGCACCA GCATTCGTTG TTCACCATGT TCGATGGAAA CATGCGCATA 
TTCATCTACA TAAGCATCGG CATCTGCGTG GTCTTCATAG TTATCGCCCT GCTAACGCTG 
AAGCTGTTCA ATGGGGTCTT TGTGAAGTCC GCGCGCACCT CGATCTATAC CAGCATTTAC 
CTTTGCCTCC TGGCCATCGA GCTGCTCTTT CTCCTGGGCA TTGAACAGAC CGAAACAAGC 
ATTTTCTGCG GCTTCATTAC TATTTTCCTA CACTGTGCCA TCCTATCGGG CACCGCCTGG 
TTCTGTTACG AAGCCTTCCA TTCGTACTCA ACGCTCACCT CGGACGAGCT CCTGCTGGAG 
GTGGACCAGA CGCCCAAGGT GAACTGCTAC TACCTCTTGT CCTACGGACT GTCGCTGAGC 
GTGGTGGCCA TCTCGCTGGT CATCGATCCC AGCACCTATA CCCAAAACGA TTATTGCGTG 
CTGATGGAGG CGAATGCCTT GTTTTATGCC ACCTTTGTAA TACCAGTGCT TGTCTTCTTT 
GTGGCTGCCA TTGGTTACAC ATTCCTCTCC TGGATTATAA TGTGCCGCAA AAGTCGCACG 
GGTCTAAAGA CCAAGGAACA TACTCGCCTC GCTAGCGTGC GGTTCGACAT ACGCTGCTCC 
TTTGTGTTCC TCTTGCTGCT CAGCGCTGTT TGGTGCTCGG CCTACTTCTA TTTGCGAGGA 
GCCAAAATGG ACGATGACAC GGCTGATGTG TATGGATACT GCTTCATCTG CTTCAACACA 
TTGCTGGGGC TCTATATCTT CGTGTTCCAT TGCATTCAAA ACGAAAAGAT CCGGCGGGAG 
TATCGGAAGT ATGTGAGACA GCACGCTTGG CTGCCCAAGT GCTTGCGCTG CTCGAAAACA 
TCAATTTCCT CGGGCATTGT TACCGGCAAT GGACCCACAG CCGGAACCCT TTGCAGCGTC 
TCCACGTCCA AGAAGCCCAA GCTGCCGTTA GGAGTGAGCG AAGAGGCGCA TGACGATCCC 
CAGCAGCAAC AGCAGACACC AGTGCCCATC ACAGAGGATG CCATTATGGG AGCCACCTCT 
GATTGTGAAC TGAACGAGGC CCAGCAAAGA AGAACCCTAA AAAGTGGCCT AATGACGGGC 
ACACTACAGG CTCCACCGCA GACCCTTGGT GGCCATGTTG TGCTCGAAAG AGGTAGCACT 
CTCCGCTCCA CTGGTCATGC CTCACCCACC AGCTCTGCCG GGTCCACACA CCTGATTTTT 
GCGCACAAGC AACAACAACA ACAGCAGCAA CAGGGACCTT TGGGCGAGTC TTACTACCAT 
CAGCCGGACT ACTACAGCTG GAAGCAACCA TCAACTGGAA CAGGAGGATT GAAAACACCG 
CGGGAGTACT ACAATAATGC GGGTGCTGCT GCATCATCGC CGCAACAGGC GCACGAGGTA 
TTCTACTGGA CTCAAAAGCC GAACAGCGGC CACAATGGCA AAAAGAAGAG GGGCGCCGGA 
GGAGTTCCCG CCTCGCCTAG TGGATCTCTG CACAGTCGCA CGGCCGCCGC CTCCCAGGTG 
CTTTTCTATC CATCGTACAA GAAGACCAAG CCTGGCCAGC CAACAGGCTA TCCGCAATAC 
GCGGAGGCGT TGGACCCACC ACTAGCCACT GGCAATGCGG CTGCCTACTA CCAGCAGCAG 
CAACAGTTGC GTCGCCAGCA GCTACATCAG CAGCAGCAAC AGCAGCAGCA GCAGCAACTC 
TCCTCGGACG AGGAGCAGGC CGAGCAACAT GCTCACCTGT TGCACCTGCA ACGACGAGCT 
GGTAGCCAGC AGCAGCTCCC TGCTCCACCG CCACACATGG CGCAGTACCA GCAGGAGTTT 
ATGCAGCGCC AGTATAGAAA TAAGCATTCC AACTGTGATC TGGGCATGGG CGATGCCTAC 
TACAACCAAG GCAGCGTCGG CGGCGCGGAT GGTGGGCCGG TCTACGAGGA GATCCTCAGC 
AACCGCAACT CGGATGTGCA GCATTACGAG GTGGGTGACT TCGATGTGGA CGAGGTGTAC 
AACAATAGCG TTGGCACTGG CGTCTTCAAC AACATGAGAG CGGCGGTGGC CGCCGGCGGT 
AGTCGGTACG GCGGCGGAAG CCTGAGTGGC GGCAGTGTCT CGTCCAGGAG CCAACAGCAG 
CAGCTCAAGA AGCAGCAGCA ACAACAGTCG CTGGCTCAGC AAAGATCGGC TCGACGCTGC 
ACGGCGGATG ACGATGACGA CGAAGACGAG GAGGAGGATG AGGAGGCAAC GGCCGCGGAG 
CAATTGCACG ACAGCGTCTG TGATGAGGAT GAGGAGGAGG ACGAGAGCGA CTTGGAGGAT 
GATGCTCATG GATTGCCACC GCAGAGCGAT GAGCGGATGC GTCGTCTGAT GGCGATGCAG 
GACGAGGATT TTAAGCGGCG GTTTCAACGT CAGCTGCGCA AACATGGAGC GCCTCTTGAC 
TACGGGGCTT TGCCACCAGG AGCAGGACCG CAACCCGAGC ACAACGGTGC GGTTTTTGGG 
GTTAGCGGCG GCGTTGGTGA GGGCTCCCTG CGTGGCGCAT TCCGGCAGCA GCAGCAGCAA 
GCACTGAATG CCAAGTCGCC AGGCGGCCGT TTGGCGGTAA ATGAGCTATT CGGTCACGGC 
AACTCGGGAC CACCGCTGCC GCCGGCGAAT CAGACGCCCG CGCAGAAGCG CCAGCAGCTA 
CAGAAACTGT CACCACAGTC CACCACATCA TCGTCGTCGC ATACATCACA CAGCAATCCC 
AATCCGCATC CCCACCAGCT AACCCATCCG CATCCCCATC AGCATCCTCC GCACCATCAG 
CAGCGCCACC TGTCGGCCAT GCTCGATGAG AACAACACGG TGCGATGTTA TCTGGAACCA 
CTGGCTAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cirl ( NM_001259276.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246205.1 Drosophila melanogaster Calcium-independent receptor for alpha-latrotoxin (Cirl), transcript variant H, mRNA.

Price & Availability↑

CloneID	OFa09296
Clone ID Related Accession (Same CDS sequence)	NM_001144148.4 , NM_001259276.2
Accession Version	NM_001259276.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Calcium-independent receptor for alpha-latrotoxin, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001259276.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTCCCA AGTCACTCAG CGTACTGAAC TCAAGATGTG CCCACAAGCA GAGCTGCGGC 
GTGTTGGCAG CCACGAGCAT GTTCGGGGAT CCCTGTCCCG GTACCCACAA GTATCTGGAG 
GCACACTACC AGTGCATAAG TGCAGCCCAA ACTTCGACGA CGACCAACAG GCCCAGTCCG 
CCGCCATGGG TGCTGAGCAA TGGTCCGCCG ATCTTTGGCA ACGGCAGTGG ACTGATCCAT 
CCGCCCGGGG TTGGAGCGGG TGCGCCGCCC CCGCCGAGAC TTCCCACACT TCCCGGAGTG 
GTGGGAATCA GTGGGAATCC CGGCCTGTTC AACGTACCAC CGCAACACAC CGCCGTCACG 
CACTCCACGC CCTCGAGCAG CACGACAGCC GTGGGCGGTG GACGTTTGAA GGGTGGGGCC 
ACCTCCACGA CGACCACCAA GCATCCGGCT GGCCGCCATG ATGGTCTGCC ACCGCCGCCG 
CAACTGCACC ACCACCACAA CCACCACGGT GAAGACACTG CCTCACCCAC CAAGCCGAGC 
AGCAAGCTGC CGGCTGGCGG TAATGCCACT TCACCATCCA ACACGAGGAT ACTCACGGGC 
GTCGGAGGTT CCGGAACTGA TGACGGAACC CTACTGACCA CAAAGAGCTC ACCCAACCGC 
CCACCGGGCA CTGCGGCCAG TGGATCCGTT GTCCCCGGGA ACGGCAGCGT GGTGCGCACC 
ATCAACAATA TTAATTTGAA CGCAGCCGGG ATGTCCGGAG GCGATGATGA GTCCAAGTTG 
TTTTGCGGCC CCACTCATGC CCGCAATTTG TACTGGAACA TGACTCGAGT GGGTGATGTG 
AATGTTCAGC CCTGTCCTGG CGGAGCAGCC GGCATCGCCA AGTGGCGTTG CGTTCTAATG 
AAGAGGATAC CCGACTCCGG CTACGATGAG TACGATGATG ACATCAGTTC GACAACTCCG 
GCACCCAGCG GTGGCGACTG TCTGCACAAC AGCAGCAGCT GCGAGCCGCC GGTGAGCATG 
GCCCACAAGG TAAACCAGCG TCTGCGCAAC TTTGAGCCCA CCTGGCATCC CGCGACACCT 
GATCTGACGC AATGCCGCAG CCTTTGGCTC AACAATCTGG AAATGCGAGT AAACCAGCGG 
GACTCCTCCT TGATCTCCAT CGCCAACGAT ATGTCCGAAG TGACCAGTAG CAAAACGCTC 
TACGGCGGCG ACATGTTGGT CACCACGAAG ATTATCCAAA CAGTGTCCGA GAAGATGATG 
CACGACAAGG AGACCTTCCC GGATCAGCGA CAGCGCGAGG CTATGATCAT GGAGTTGTTG 
CATTGTGTGG TCAAAACCGG CTCCAACCTG CTGGACGAAT CGCAGCTGTC CTCGTGGTTG 
GATCTCAATC CGGAGGACCA AATGCGTGTA GCCACATCCT TGCTAACTGG CCTGGAATAC 
AATGCCTTTC TGCTGGCGGA TACGATCATC AGGGAGCGCA GCGTGGTGCA AAAAGTCAAA 
AATATATTGC TCTCCGTTCG AGTTCTGGAA ACCAAGACTA TCCAGTCCAG CGTGGTCTTC 
CCAGATTCGG ATCAGTGGCC CTTGAGTTCG GATCGTATTG AGCTGCCACG AGCTGCTCTA 
ATAGATAATA GTGAAGGCGG TCTGGTGCGA ATTGTATTCG CCGCCTTCGA TCGCCTGGAA 
TCCATTCTAA AGCCCAGCTA TGATCACTTC GATCTCAAGA GCTCCCGCAG TTACGTTCGC 
AATACAGCCA TCCTGAGCAA CGACAGCGAT GTCAACGCGG GGGAGATCCA ACAGCGCCTA 
CGCATCCTGA ACAGCAAGGT GATCTCGGCC AGCTTGGGCA AGGGGCGTCA CATACAACTC 
TCCCAGCCCA TAACCCTGAC ACTGAAACAT CTGAAGACCG AGAATGTAAC GAATCCCACC 
TGCGTGTTCT GGAACTATAT TGACCATGCG TGGTCTGCCA ACGGATGCAG TCTGGAGTCC 
ACTAACCGCA CGCACAGCGT CTGCAGTTGC AACCACCTGA CAAACTTTGC CATACTAATG 
GACGTTGTGG ATGAGCACCA GCATTCGTTG TTCACCATGT TCGATGGAAA CATGCGCATA 
TTCATCTACA TAAGCATCGG CATCTGCGTG GTCTTCATAG TTATCGCCCT GCTAACGCTG 
AAGCTGTTCA ATGGGGTCTT TGTGAAGTCC GCGCGCACCT CGATCTATAC CAGCATTTAC 
CTTTGCCTCC TGGCCATCGA GCTGCTCTTT CTCCTGGGCA TTGAACAGAC CGAAACAAGC 
ATTTTCTGCG GCTTCATTAC TATTTTCCTA CACTGTGCCA TCCTATCGGG CACCGCCTGG 
TTCTGTTACG AAGCCTTCCA TTCGTACTCA ACGCTCACCT CGGACGAGCT CCTGCTGGAG 
GTGGACCAGA CGCCCAAGGT GAACTGCTAC TACCTCTTGT CCTACGGACT GTCGCTGAGC 
GTGGTGGCCA TCTCGCTGGT CATCGATCCC AGCACCTATA CCCAAAACGA TTATTGCGTG 
CTGATGGAGG CGAATGCCTT GTTTTATGCC ACCTTTGTAA TACCAGTGCT TGTCTTCTTT 
GTGGCTGCCA TTGGTTACAC ATTCCTCTCC TGGATTATAA TGTGCCGCAA AAGTCGCACG 
GGTCTAAAGA CCAAGGAACA TACTCGCCTC GCTAGCGTGC GGTTCGACAT ACGCTGCTCC 
TTTGTGTTCC TCTTGCTGCT CAGCGCTGTT TGGTGCTCGG CCTACTTCTA TTTGCGAGGA 
GCCAAAATGG ACGATGACAC GGCTGATGTG TATGGATACT GCTTCATCTG CTTCAACACA 
TTGCTGGGGC TCTATATCTT CGTGTTCCAT TGCATTCAAA ACGAAAAGAT CCGGCGGGAG 
TATCGGAAGT ATGTGAGACA GCACGCTTGG CTGCCCAAGT GCTTGCGCTG CTCGAAAACA 
TCAATTTCCT CGGGCATTGT TACCGGCAAT GGACCCACAG CCGGAACCCT TTGCAGCGTC 
TCCACGTCCA AGAAGCCCAA GCTGCCGTTA GGAGTGAGCG AAGAGGCGCA TGACGATCCC 
CAGCAGCAAC AGCAGACACC AGTGCCCATC ACAGAGGATG CCATTATGGG AGCCACCTCT 
GATTGTGAAC TGAACGAGGC CCAGCAAAGA AGAACCCTAA AAAGTGGCCT AATGACGGGC 
ACACTACAGG CTCCACCGCA GACCCTTGGT GGCCATGTTG TGCTCGAAAG AGGTAGCACT 
CTCCGCTCCA CTGGTCATGC CTCACCCACC AGCTCTGCCG GGTCCACACA CCTGATTTTT 
GCGCACAAGC AACAACAACA ACAGCAGCAA CAGGGACCTT TGGGCGAGTC TTACTACCAT 
CAGCCGGACT ACTACAGCTG GAAGCAACCA TCAACTGGAA CAGGAGGATT GAAAACACCG 
CGGGAGTACT ACAATAATGC GGGTGCTGCT GCATCATCGC CGCAACAGGC GCACGAGGTA 
TTCTACTGGA CTCAAAAGCC GAACAGCGGC CACAATGGCA AAAAGAAGAG GGGCGCCGGA 
GGAGTTCCCG CCTCGCCTAG TGGATCTCTG CACAGTCGCA CGGCCGCCGC CTCCCAGGTG 
CTTTTCTATC CATCGTACAA GAAGACCAAG CCTGGCCAGC CAACAGGCTA TCCGCAATAC 
GCGGAGGCGT TGGACCCACC ACTAGCCACT GGCAATGCGG CTGCCTACTA CCAGCAGCAG 
CAACAGTTGC GTCGCCAGCA GCTACATCAG CAGCAGCAAC AGCAGCAGCA GCAGCAACTC 
TCCTCGGACG AGGAGCAGGC CGAGCAACAT GCTCACCTGT TGCACCTGCA ACGACGAGCT 
GGTAGCCAGC AGCAGCTCCC TGCTCCACCG CCACACATGG CGCAGTACCA GCAGGAGTTT 
ATGCAGCGCC AGTATAGAAA TAAGCATTCC AACTGTGATC TGGGCATGGG CGATGCCTAC 
TACAACCAAG GCAGCGTCGG CGGCGCGGAT GGTGGGCCGG TCTACGAGGA GATCCTCAGC 
AACCGCAACT CGGATGTGCA GCATTACGAG GTGGGTGACT TCGATGTGGA CGAGGTGTAC 
AACAATAGCG TTGGCACTGG CGTCTTCAAC AACATGAGAG CGGCGGTGGC CGCCGGCGGT 
AGTCGGTACG GCGGCGGAAG CCTGAGTGGC GGCAGTGTCT CGTCCAGGAG CCAACAGCAG 
CAGCTCAAGA AGCAGCAGCA ACAACAGTCG CTGGCTCAGC AAAGATCGGC TCGACGCTGC 
ACGGCGGATG ACGATGACGA CGAAGACGAG GAGGAGGATG AGGAGGCAAC GGCCGCGGAG 
CAATTGCACG ACAGCGTCTG TGATGAGGAT GAGGAGGAGG ACGAGAGCGA CTTGGAGGAT 
GATGCTCATG GATTGCCACC GCAGAGCGAT GAGCGGATGC GTCGTCTGAT GGCGATGCAG 
GACGAGGATT TTAAGCGGCG GTTTCAACGT CAGCTGCGCA AACATGGAGC GCCTCTTGAC 
TACGGGGCTT TGCCACCAGG AGCAGGACCG CAACCCGAGC ACAACGGTGC GGTTTTTGGG 
GTTAGCGGCG GCGTTGGTGA GGGCTCCCTG CGTGGCGCAT TCCGGCAGCA GCAGCAGCAA 
GCACTGAATG CCAAGTCGCC AGGCGGCCGT TTGGCGGTAA ATGAGCTATT CGGTCACGGC 
AACTCGGGAC CACCGCTGCC GCCGGCGAAT CAGACGCCCG CGCAGAAGCG CCAGCAGCTA 
CAGAAACTGT CACCACAGTC CACCACATCA TCGTCGTCGC ATACATCACA CAGCAATCCC 
AATCCGCATC CCCACCAGCT AACCCATCCG CATCCCCATC AGCATCCTCC GCACCATCAG 
CAGCGCCACC TGTCGGCCAT GCTCGATGAG AACAACACGG TGCGATGTTA TCTGGAACCA 
CTGGCTAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246205.1
CDS	1178..6109
Translation	MFPKSLSVLNSRCAHKQSCGVLAATSMFGDPCPGTHKYLEAHYQCISAAQTSTTTNRPSPPPWVLSNGPPIFGNGSGLIHPPGVGAGAPPPPRLPTLPGVVGISGNPGLFNVPPQHTAVTHSTPSSSTTAVGGGRLKGGATSTTTTKHPAGRHDGLPPPPQLHHHHNHHGEDTASPTKPSSKLPAGGNATSPSNTRILTGVGGSGTDDGTLLTTKSSPNRPPGTAASGSVVPGNGSVVRTINNINLNAAGMSGGDDESKLFCGPTHARNLYWNMTRVGDVNVQPCPGGAAGIAKWRCVLMKRIPDSGYDEYDDDISSTTPAPSGGDCLHNSSSCEPPVSMAHKVNQRLRNFEPTWHPATPDLTQCRSLWLNNLEMRVNQRDSSLISIANDMSEVTSSKTLYGGDMLVTTKIIQTVSEKMMHDKETFPDQRQREAMIMELLHCVVKTGSNLLDESQLSSWLDLNPEDQMRVATSLLTGLEYNAFLLADTIIRERSVVQKVKNILLSVRVLETKTIQSSVVFPDSDQWPLSSDRIELPRAALIDNSEGGLVRIVFAAFDRLESILKPSYDHFDLKSSRSYVRNTAILSNDSDVNAGEIQQRLRILNSKVISASLGKGRHIQLSQPITLTLKHLKTENVTNPTCVFWNYIDHAWSANGCSLESTNRTHSVCSCNHLTNFAILMDVVDEHQHSLFTMFDGNMRIFIYISIGICVVFIVIALLTLKLFNGVFVKSARTSIYTSIYLCLLAIELLFLLGIEQTETSIFCGFITIFLHCAILSGTAWFCYEAFHSYSTLTSDELLLEVDQTPKVNCYYLLSYGLSLSVVAISLVIDPSTYTQNDYCVLMEANALFYATFVIPVLVFFVAAIGYTFLSWIIMCRKSRTGLKTKEHTRLASVRFDIRCSFVFLLLLSAVWCSAYFYLRGAKMDDDTADVYGYCFICFNTLLGLYIFVFHCIQNEKIRREYRKYVRQHAWLPKCLRCSKTSISSGIVTGNGPTAGTLCSVSTSKKPKLPLGVSEEAHDDPQQQQQTPVPITEDAIMGATSDCELNEAQQRRTLKSGLMTGTLQAPPQTLGGHVVLERGSTLRSTGHASPTSSAGSTHLIFAHKQQQQQQQQGPLGESYYHQPDYYSWKQPSTGTGGLKTPREYYNNAGAAASSPQQAHEVFYWTQKPNSGHNGKKKRGAGGVPASPSGSLHSRTAAASQVLFYPSYKKTKPGQPTGYPQYAEALDPPLATGNAAAYYQQQQQLRRQQLHQQQQQQQQQQLSSDEEQAEQHAHLLHLQRRAGSQQQLPAPPPHMAQYQQEFMQRQYRNKHSNCDLGMGDAYYNQGSVGGADGGPVYEEILSNRNSDVQHYEVGDFDVDEVYNNSVGTGVFNNMRAAVAAGGSRYGGGSLSGGSVSSRSQQQQLKKQQQQQSLAQQRSARRCTADDDDDEDEEEDEEATAAEQLHDSVCDEDEEEDESDLEDDAHGLPPQSDERMRRLMAMQDEDFKRRFQRQLRKHGAPLDYGALPPGAGPQPEHNGAVFGVSGGVGEGSLRGAFRQQQQQALNAKSPGGRLAVNELFGHGNSGPPLPPANQTPAQKRQQLQKLSPQSTTSSSSHTSHSNPNPHPHQLTHPHPHQHPPHHQQRHLSAMLDENNTVRCYLEPLAK

Target ORF information:

RefSeq Version	NM_001259276.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Calcium-independent receptor for alpha-latrotoxin (Cirl), transcript variant H, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259276.2

ORF Insert Sequence:

ATGTTTCCCA AGTCACTCAG CGTACTGAAC TCAAGATGTG CCCACAAGCA GAGCTGCGGC 
GTGTTGGCAG CCACGAGCAT GTTCGGGGAT CCCTGTCCCG GTACCCACAA GTATCTGGAG 
GCACACTACC AGTGCATAAG TGCAGCCCAA ACTTCGACGA CGACCAACAG GCCCAGTCCG 
CCGCCATGGG TGCTGAGCAA TGGTCCGCCG ATCTTTGGCA ACGGCAGTGG ACTGATCCAT 
CCGCCCGGGG TTGGAGCGGG TGCGCCGCCC CCGCCGAGAC TTCCCACACT TCCCGGAGTG 
GTGGGAATCA GTGGGAATCC CGGCCTGTTC AACGTACCAC CGCAACACAC CGCCGTCACG 
CACTCCACGC CCTCGAGCAG CACGACAGCC GTGGGCGGTG GACGTTTGAA GGGTGGGGCC 
ACCTCCACGA CGACCACCAA GCATCCGGCT GGCCGCCATG ATGGTCTGCC ACCGCCGCCG 
CAACTGCACC ACCACCACAA CCACCACGGT GAAGACACTG CCTCACCCAC CAAGCCGAGC 
AGCAAGCTGC CGGCTGGCGG TAATGCCACT TCACCATCCA ACACGAGGAT ACTCACGGGC 
GTCGGAGGTT CCGGAACTGA TGACGGAACC CTACTGACCA CAAAGAGCTC ACCCAACCGC 
CCACCGGGCA CTGCGGCCAG TGGATCCGTT GTCCCCGGGA ACGGCAGCGT GGTGCGCACC 
ATCAACAATA TTAATTTGAA CGCAGCCGGG ATGTCCGGAG GCGATGATGA GTCCAAGTTG 
TTTTGCGGCC CCACTCATGC CCGCAATTTG TACTGGAACA TGACTCGAGT GGGTGATGTG 
AATGTTCAGC CCTGTCCTGG CGGAGCAGCC GGCATCGCCA AGTGGCGTTG CGTTCTAATG 
AAGAGGATAC CCGACTCCGG CTACGATGAG TACGATGATG ACATCAGTTC GACAACTCCG 
GCACCCAGCG GTGGCGACTG TCTGCACAAC AGCAGCAGCT GCGAGCCGCC GGTGAGCATG 
GCCCACAAGG TAAACCAGCG TCTGCGCAAC TTTGAGCCCA CCTGGCATCC CGCGACACCT 
GATCTGACGC AATGCCGCAG CCTTTGGCTC AACAATCTGG AAATGCGAGT AAACCAGCGG 
GACTCCTCCT TGATCTCCAT CGCCAACGAT ATGTCCGAAG TGACCAGTAG CAAAACGCTC 
TACGGCGGCG ACATGTTGGT CACCACGAAG ATTATCCAAA CAGTGTCCGA GAAGATGATG 
CACGACAAGG AGACCTTCCC GGATCAGCGA CAGCGCGAGG CTATGATCAT GGAGTTGTTG 
CATTGTGTGG TCAAAACCGG CTCCAACCTG CTGGACGAAT CGCAGCTGTC CTCGTGGTTG 
GATCTCAATC CGGAGGACCA AATGCGTGTA GCCACATCCT TGCTAACTGG CCTGGAATAC 
AATGCCTTTC TGCTGGCGGA TACGATCATC AGGGAGCGCA GCGTGGTGCA AAAAGTCAAA 
AATATATTGC TCTCCGTTCG AGTTCTGGAA ACCAAGACTA TCCAGTCCAG CGTGGTCTTC 
CCAGATTCGG ATCAGTGGCC CTTGAGTTCG GATCGTATTG AGCTGCCACG AGCTGCTCTA 
ATAGATAATA GTGAAGGCGG TCTGGTGCGA ATTGTATTCG CCGCCTTCGA TCGCCTGGAA 
TCCATTCTAA AGCCCAGCTA TGATCACTTC GATCTCAAGA GCTCCCGCAG TTACGTTCGC 
AATACAGCCA TCCTGAGCAA CGACAGCGAT GTCAACGCGG GGGAGATCCA ACAGCGCCTA 
CGCATCCTGA ACAGCAAGGT GATCTCGGCC AGCTTGGGCA AGGGGCGTCA CATACAACTC 
TCCCAGCCCA TAACCCTGAC ACTGAAACAT CTGAAGACCG AGAATGTAAC GAATCCCACC 
TGCGTGTTCT GGAACTATAT TGACCATGCG TGGTCTGCCA ACGGATGCAG TCTGGAGTCC 
ACTAACCGCA CGCACAGCGT CTGCAGTTGC AACCACCTGA CAAACTTTGC CATACTAATG 
GACGTTGTGG ATGAGCACCA GCATTCGTTG TTCACCATGT TCGATGGAAA CATGCGCATA 
TTCATCTACA TAAGCATCGG CATCTGCGTG GTCTTCATAG TTATCGCCCT GCTAACGCTG 
AAGCTGTTCA ATGGGGTCTT TGTGAAGTCC GCGCGCACCT CGATCTATAC CAGCATTTAC 
CTTTGCCTCC TGGCCATCGA GCTGCTCTTT CTCCTGGGCA TTGAACAGAC CGAAACAAGC 
ATTTTCTGCG GCTTCATTAC TATTTTCCTA CACTGTGCCA TCCTATCGGG CACCGCCTGG 
TTCTGTTACG AAGCCTTCCA TTCGTACTCA ACGCTCACCT CGGACGAGCT CCTGCTGGAG 
GTGGACCAGA CGCCCAAGGT GAACTGCTAC TACCTCTTGT CCTACGGACT GTCGCTGAGC 
GTGGTGGCCA TCTCGCTGGT CATCGATCCC AGCACCTATA CCCAAAACGA TTATTGCGTG 
CTGATGGAGG CGAATGCCTT GTTTTATGCC ACCTTTGTAA TACCAGTGCT TGTCTTCTTT 
GTGGCTGCCA TTGGTTACAC ATTCCTCTCC TGGATTATAA TGTGCCGCAA AAGTCGCACG 
GGTCTAAAGA CCAAGGAACA TACTCGCCTC GCTAGCGTGC GGTTCGACAT ACGCTGCTCC 
TTTGTGTTCC TCTTGCTGCT CAGCGCTGTT TGGTGCTCGG CCTACTTCTA TTTGCGAGGA 
GCCAAAATGG ACGATGACAC GGCTGATGTG TATGGATACT GCTTCATCTG CTTCAACACA 
TTGCTGGGGC TCTATATCTT CGTGTTCCAT TGCATTCAAA ACGAAAAGAT CCGGCGGGAG 
TATCGGAAGT ATGTGAGACA GCACGCTTGG CTGCCCAAGT GCTTGCGCTG CTCGAAAACA 
TCAATTTCCT CGGGCATTGT TACCGGCAAT GGACCCACAG CCGGAACCCT TTGCAGCGTC 
TCCACGTCCA AGAAGCCCAA GCTGCCGTTA GGAGTGAGCG AAGAGGCGCA TGACGATCCC 
CAGCAGCAAC AGCAGACACC AGTGCCCATC ACAGAGGATG CCATTATGGG AGCCACCTCT 
GATTGTGAAC TGAACGAGGC CCAGCAAAGA AGAACCCTAA AAAGTGGCCT AATGACGGGC 
ACACTACAGG CTCCACCGCA GACCCTTGGT GGCCATGTTG TGCTCGAAAG AGGTAGCACT 
CTCCGCTCCA CTGGTCATGC CTCACCCACC AGCTCTGCCG GGTCCACACA CCTGATTTTT 
GCGCACAAGC AACAACAACA ACAGCAGCAA CAGGGACCTT TGGGCGAGTC TTACTACCAT 
CAGCCGGACT ACTACAGCTG GAAGCAACCA TCAACTGGAA CAGGAGGATT GAAAACACCG 
CGGGAGTACT ACAATAATGC GGGTGCTGCT GCATCATCGC CGCAACAGGC GCACGAGGTA 
TTCTACTGGA CTCAAAAGCC GAACAGCGGC CACAATGGCA AAAAGAAGAG GGGCGCCGGA 
GGAGTTCCCG CCTCGCCTAG TGGATCTCTG CACAGTCGCA CGGCCGCCGC CTCCCAGGTG 
CTTTTCTATC CATCGTACAA GAAGACCAAG CCTGGCCAGC CAACAGGCTA TCCGCAATAC 
GCGGAGGCGT TGGACCCACC ACTAGCCACT GGCAATGCGG CTGCCTACTA CCAGCAGCAG 
CAACAGTTGC GTCGCCAGCA GCTACATCAG CAGCAGCAAC AGCAGCAGCA GCAGCAACTC 
TCCTCGGACG AGGAGCAGGC CGAGCAACAT GCTCACCTGT TGCACCTGCA ACGACGAGCT 
GGTAGCCAGC AGCAGCTCCC TGCTCCACCG CCACACATGG CGCAGTACCA GCAGGAGTTT 
ATGCAGCGCC AGTATAGAAA TAAGCATTCC AACTGTGATC TGGGCATGGG CGATGCCTAC 
TACAACCAAG GCAGCGTCGG CGGCGCGGAT GGTGGGCCGG TCTACGAGGA GATCCTCAGC 
AACCGCAACT CGGATGTGCA GCATTACGAG GTGGGTGACT TCGATGTGGA CGAGGTGTAC 
AACAATAGCG TTGGCACTGG CGTCTTCAAC AACATGAGAG CGGCGGTGGC CGCCGGCGGT 
AGTCGGTACG GCGGCGGAAG CCTGAGTGGC GGCAGTGTCT CGTCCAGGAG CCAACAGCAG 
CAGCTCAAGA AGCAGCAGCA ACAACAGTCG CTGGCTCAGC AAAGATCGGC TCGACGCTGC 
ACGGCGGATG ACGATGACGA CGAAGACGAG GAGGAGGATG AGGAGGCAAC GGCCGCGGAG 
CAATTGCACG ACAGCGTCTG TGATGAGGAT GAGGAGGAGG ACGAGAGCGA CTTGGAGGAT 
GATGCTCATG GATTGCCACC GCAGAGCGAT GAGCGGATGC GTCGTCTGAT GGCGATGCAG 
GACGAGGATT TTAAGCGGCG GTTTCAACGT CAGCTGCGCA AACATGGAGC GCCTCTTGAC 
TACGGGGCTT TGCCACCAGG AGCAGGACCG CAACCCGAGC ACAACGGTGC GGTTTTTGGG 
GTTAGCGGCG GCGTTGGTGA GGGCTCCCTG CGTGGCGCAT TCCGGCAGCA GCAGCAGCAA 
GCACTGAATG CCAAGTCGCC AGGCGGCCGT TTGGCGGTAA ATGAGCTATT CGGTCACGGC 
AACTCGGGAC CACCGCTGCC GCCGGCGAAT CAGACGCCCG CGCAGAAGCG CCAGCAGCTA 
CAGAAACTGT CACCACAGTC CACCACATCA TCGTCGTCGC ATACATCACA CAGCAATCCC 
AATCCGCATC CCCACCAGCT AACCCATCCG CATCCCCATC AGCATCCTCC GCACCATCAG 
CAGCGCCACC TGTCGGCCAT GCTCGATGAG AACAACACGG TGCGATGTTA TCTGGAACCA 
CTGGCTAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cirl ( NM_001273878.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260807.1 Drosophila melanogaster Calcium-independent receptor for alpha-latrotoxin (Cirl), transcript variant I, mRNA.

Price & Availability↑

CloneID	OFa09297
Clone ID Related Accession (Same CDS sequence)	NM_001273878.1
Accession Version	NM_001273878.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Calcium-independent receptor for alpha-latrotoxin, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTACCAA CCATATTGTC AATATCCTAT GAGCATATAT CGTTAGATCT GTCCAAGTAC 
CAAACCGCCT ACGCCTGCGA AGGTAAGAAA CTGACCATCG AGTGCGATCC CGGCGATGTG 
ATCAACCTCA TTCGGGCCAA CTATGGCCGC TTCTCGATTA CCATCTGCAA TGACCACGGG 
AATGTGGAGT GGAGTGTTAA CTGCATGTTT CCCAAGTCAC TCAGCGTACT GAACTCAAGA 
TGTGCCCACA AGCAGAGCTG CGGCGTGTTG GCAGCCACGA GCATGTTCGG GGATCCCTGT 
CCCGGTACCC ACAAGTATCT GGAGGCACAC TACCAGTGCA TAAGTGCAGC CCAAACTTCG 
ACGACGACCA ACAGGCCCAG TCCGCCGCCA TGGGTGCTGA GCAATGGTCC GCCGATCTTT 
GGCAACGGCA GTGGACTGAT CCATCCGCCC GGGGTTGGAG CGGGTGCGCC GCCCCCGCCG 
AGACTTCCCA CACTTCCCGG AGTGGTGGGA ATCAGTGGGA ATCCCGGCCT GTTCAACGTA 
CCACCGCAAC ACACCGCCGT CACGCACTCC ACGCCCTCGA GCAGCACGAC AGCCGTGGGC 
GGTGGACGTT TGAAGGGTGG GGCCACCTCC ACGACGACCA CCAAGCATCC GGCTGGCCGC 
CATGATGGTC TGCCACCGCC GCCGCAACTG CACCACCACC ACAACCACCA CGGTGAAGAC 
ACTGCCTCAC CCACCAAGCC GAGCAGCAAG CTGCCGGCTG GCGGTAATGC CACTTCACCA 
TCCAACACGA GGATACTCAC GGGCGTCGGA GGTTCCGGAA CTGATGACGG AACCCTACTG 
ACCACAAAGA GCTCACCCAA CCGCCCACCG GGCACTGCGG CCAGTGGATC CGTTGTCCCC 
GGGAACGGCA GCGTGGTGCG CACCATCAAC AATATTAATT TGAACGCAGC CGGGATGTCC 
GGAGGCGATG ATGAGTCCAA GTTGTTTTGC GGCCCCACTC ATGCCCGCAA TTTGTACTGG 
AACATGACTC GAGTGGGTGA TGTGAATGTT CAGCCCTGTC CTGGCGGAGC AGCCGGCATC 
GCCAAGTGGC GTTGCGTTCT AATGAAGAGG ATACCCGACT CCGGCTACGA TGAGTACGAT 
GATGACATCA GTTCGACAAC TCCGGCACCC AGCGGTGGCG ACTGTCTGCA CAACAGCAGC 
AGCTGCGAGC CGCCGGTGAG CATGGCCCAC AAGGTAAACC AGCGTCTGCG CAACTTTGAG 
CCCACCTGGC ATCCCGCGAC ACCTGATCTG ACGCAATGCC GCAGCCTTTG GCTCAACAAT 
CTGGAAATGC GAGTAAACCA GCGGGACTCC TCCTTGATCT CCATCGCCAA CGATATGTCC 
GAAGTGACCA GTAGCAAAAC GCTCTACGGC GGCGACATGT TGGTCACCAC GAAGATTATC 
CAAACAGTGT CCGAGAAGAT GATGCACGAC AAGGAGACCT TCCCGGATCA GCGACAGCGC 
GAGGCTATGA TCATGGAGTT GTTGCATTGT GTGGTCAAAA CCGGCTCCAA CCTGCTGGAC 
GAATCGCAGC TGTCCTCGTG GTTGGATCTC AATCCGGAGG ACCAAATGCG TGTAGCCACA 
TCCTTGCTAA CTGGCCTGGA ATACAATGCC TTTCTGCTGG CGGATACGAT CATCAGGGAG 
CGCAGCGTGG TGCAAAAAGT CAAAAATATA TTGCTCTCCG TTCGAGTTCT GGAAACCAAG 
ACTATCCAGT CCAGCGTGGT CTTCCCAGAT TCGGATCAGT GGCCCTTGAG TTCGGATCGT 
ATTGAGCTGC CACGAGCTGC TCTAATAGAT AATAGTGAAG GCGGTCTGGT GCGAATTGTA 
TTCGCCGCCT TCGATCGCCT GGAATCCATT CTAAAGCCCA GCTATGATCA CTTCGATCTC 
AAGAGCTCCC GCAGTTACGT TCGCAATACA GCCATCCTGA GCAACGACAG CGATGTCAAC 
GCGGGGGAGA TCCAACAGCG CCTACGCATC CTGAACAGCA AGGTGATCTC GGCCAGCTTG 
GGCAAGGGGC GTCACATACA ACTCTCCCAG CCCATAACCC TGACACTGAA ACATCTGAAG 
ACCGAGAATG TAACGAATCC CACCTGCGTG TTCTGGAACT ATATTGACCA TGCGTGGTCT 
GCCAACGGAT GCAGTCTGGA GTCCACTAAC CGCACGCACA GCGTCTGCAG TTGCAACCAC 
CTGACAAACT TTGCCATACT AATGGACGTT GTGGATGAGC ACCAGCATTC GTTGTTCACC 
ATGTTCGATG GAAACATGCG CATATTCATC TACATAAGCA TCGGCATCTG CGTGGTCTTC 
ATAGTTATCG CCCTGCTAAC GCTGAAGCTG TTCAATGGGG TCTTTGTGAA GTCCGCGCGC 
ACCTCGATCT ATACCAGCAT TTACCTTTGC CTCCTGGCCA TCGAGCTGCT CTTTCTCCTG 
GGCATTGAAC AGACCGAAAC AAGCATTTTC TGCGGCTTCA TTACTATTTT CCTACACTGT 
GCCATCCTAT CGGGCACCGC CTGGTTCTGT TACGAAGCCT TCCATTCGTA CTCAACGCTC 
ACCTCGGACG AGCTCCTGCT GGAGGTGGAC CAGACGCCCA AGGTGAACTG CTACTACCTC 
TTGTCCTACG GACTGTCGCT GAGCGTGGTG GCCATCTCGC TGGTCATCGA TCCCAGCACC 
TATACCCAAA ACGATTATTG CGTGCTGATG GAGGCGAATG CCTTGTTTTA TGCCACCTTT 
GTAATACCAG TGCTTGTCTT CTTTGTGGCT GCCATTGGTT ACACATTCCT CTCCTGGATT 
ATAATGTGCC GCAAAAGTCG CACGGGTCTA AAGACCAAGG AACATACTCG CCTCGCTAGC 
GTGCGGTTCG ACATACGCTG CTCCTTTGTG TTCCTCTTGC TGCTCAGCGC TGTTTGGTGC 
TCGGCCTACT TCTATTTGCG AGGAGCCAAA ATGGACGATG ACACGGCTGA TGTGTATGGA 
TACTGCTTCA TCTGCTTCAA CACATTGCTG GGGCTCTATA TCTTCGTGTT CCATTGCATT 
CAAAACGAAA AGATCCGGCG GGAGTATCGG AAGTATGTGA GACAGCACGC TTGGCTGCCC 
AAGTGCTTGC GCTGCTCGAA AACATCAATT TCCTCGGGCA TTGTTACCGG CAATGGACCC 
ACAGCCGGAA CCCTTTGCAG CGTCTCCACG TCCAAGAAGC CCAAGCTGCC GTTAGGAGTG 
AGCGAAGAGG CGCATGACGA TCCCCAGCAG CAACAGCAGA CACCAGTGCC CATCACAGAG 
GATGCCATTA TGGGAGCCAC CTCTGATTGT GAACTGAACG AGGCCCAGCA AAGAAGAACC 
CTAAAAAGTG GCCTAATGAC GGGCACACTA CAGGCTCCAC CGCAGACCCT TGGTGGCCAT 
GTTGTGCTCG AAAGAGGTAG CACTCTCCGC TCCACTGGTC ATGCCTCACC CACCAGCTCT 
GCCGGGTCCA CACACCTGAT TTTTGCGCAC AAGCAACAAC AACAACAGCA GCAACAGGGA 
CCTTTGGGCG AGTCTTACTA CCATCAGCCG GACTACTACA GCTGGAAGCA ACCATCAACT 
GGAACAGGAG GATTGAAAAC ACCGCGGGAG TACTACAATA ATGCGGGTGC TGCTGCATCA 
TCGCCGCAAC AGGCGCACGA GGTATTCTAC TGGACTCAAA AGCCGAACAG CGGCCACAAT 
GGCAAAAAGA AGAGGGGCGC CGGAGGAGTT CCCGCCTCGC CTAGTGGATC TCTGCACAGT 
CGCACGGCCG CCGCCTCCCA GGTGCTTTTC TATCCATCGT ACAAGAAGAC CAAGCCTGGC 
CAGCCAACAG GCTATCCGCA ATACGCGGAG GCGTTGGACC CACCACTAGC CACTGGCAAT 
GCGGCTGCCT ACTACCAGCA GCAGCAACAG TTGCGTCGCC AGCAGCTACA TCAGCAGCAG 
CAACAGCAGC AGCAGCAGCA ACTCTCCTCG GACGAGGAGC AGGCCGAGCA ACATGCTCAC 
CTGTTGCACC TGCAACGACG AGCTGGTAGC CAGCAGCAGC TCCCTGCTCC ACCGCCACAC 
ATGGCGCAGT ACCAGCAGGA GTTTATGCAG CGCCAGTATA GAAATAAGCA TTCCAACTGT 
GATCTGGGCA TGGGCGATGC CTACTACAAC CAAGGCAGCG TCGGCGGCGC GGATGGTGGG 
CCGGTCTACG AGGAGATCCT CAGCAACCGC AACTCGGATG TGCAGCATTA CGAGGTGGGT 
GACTTCGATG TGGACGAGGT GTACAACAAT AGCGTTGGCA CTGGCGTCTT CAACAACATG 
AGAGCGGCGG TGGCCGCCGG CGGTAGTCGG TACGGCGGCG GAAGCCTGAG TGGCGGCAGT 
GTCTCGTCCA GGAGCCAACA GCAGCAGCTC AAGAAGCAGC AGCAACAACA GTCGCTGGCT 
CAGCAAAGAT CGGCTCGACG CTGCACGGCG GATGACGATG ACGACGAAGA CGAGGAGGAG 
GATGAGGAGG CAACGGCCGC GGAGCAATTG CACGACAGCG TCTGTGATGA GGATGAGGAG 
GAGGACGAGA GCGACTTGGA GGATGATGCT CATGGATTGC CACCGCAGAG CGATGAGCGG 
ATGCGTCGTC TGATGGCGAT GCAGGACGAG GATTTTAAGC GGCGGTTTCA ACGTCAGCTG 
CGCAAACATG GAGCGCCTCT TGACTACGGG GCTTTGCCAC CAGGAGCAGG ACCGCAACCC 
GAGCACAACG GTGCGGTTTT TGGGGTTAGC GGCGGCGTTG GTGAGGGCTC CCTGCGTGGC 
GCATTCCGGC AGCAGCAGCA GCAAGCACTG AATGCCAAGT CGCCAGGCGG CCGTTTGGCG 
GTAAATGAGC TATTCGGTCA CGGCAACTCG GGACCACCGC TGCCGCCGGC GAATCAGACG 
CCCGCGCAGA AGCGCCAGCA GCTACAGAAA CTGTCACCAC AGTCCACCAC ATCATCGTCG 
TCGCATACAT CACACAGCAA TCCCAATCCG CATCCCCACC AGCTAACCCA TCCGCATCCC 
CATCAGCATC CTCCGCACCA TCAGCAGCGC CACCTGTCGG CCATGCTCGA TGAGAACAAC 
ACGGTGCGAT GTTATCTGGA ACCACTGGCT AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260807.1
CDS	800..5935
Translation	MLPTILSISYEHISLDLSKYQTAYACEGKKLTIECDPGDVINLIRANYGRFSITICNDHGNVEWSVNCMFPKSLSVLNSRCAHKQSCGVLAATSMFGDPCPGTHKYLEAHYQCISAAQTSTTTNRPSPPPWVLSNGPPIFGNGSGLIHPPGVGAGAPPPPRLPTLPGVVGISGNPGLFNVPPQHTAVTHSTPSSSTTAVGGGRLKGGATSTTTTKHPAGRHDGLPPPPQLHHHHNHHGEDTASPTKPSSKLPAGGNATSPSNTRILTGVGGSGTDDGTLLTTKSSPNRPPGTAASGSVVPGNGSVVRTINNINLNAAGMSGGDDESKLFCGPTHARNLYWNMTRVGDVNVQPCPGGAAGIAKWRCVLMKRIPDSGYDEYDDDISSTTPAPSGGDCLHNSSSCEPPVSMAHKVNQRLRNFEPTWHPATPDLTQCRSLWLNNLEMRVNQRDSSLISIANDMSEVTSSKTLYGGDMLVTTKIIQTVSEKMMHDKETFPDQRQREAMIMELLHCVVKTGSNLLDESQLSSWLDLNPEDQMRVATSLLTGLEYNAFLLADTIIRERSVVQKVKNILLSVRVLETKTIQSSVVFPDSDQWPLSSDRIELPRAALIDNSEGGLVRIVFAAFDRLESILKPSYDHFDLKSSRSYVRNTAILSNDSDVNAGEIQQRLRILNSKVISASLGKGRHIQLSQPITLTLKHLKTENVTNPTCVFWNYIDHAWSANGCSLESTNRTHSVCSCNHLTNFAILMDVVDEHQHSLFTMFDGNMRIFIYISIGICVVFIVIALLTLKLFNGVFVKSARTSIYTSIYLCLLAIELLFLLGIEQTETSIFCGFITIFLHCAILSGTAWFCYEAFHSYSTLTSDELLLEVDQTPKVNCYYLLSYGLSLSVVAISLVIDPSTYTQNDYCVLMEANALFYATFVIPVLVFFVAAIGYTFLSWIIMCRKSRTGLKTKEHTRLASVRFDIRCSFVFLLLLSAVWCSAYFYLRGAKMDDDTADVYGYCFICFNTLLGLYIFVFHCIQNEKIRREYRKYVRQHAWLPKCLRCSKTSISSGIVTGNGPTAGTLCSVSTSKKPKLPLGVSEEAHDDPQQQQQTPVPITEDAIMGATSDCELNEAQQRRTLKSGLMTGTLQAPPQTLGGHVVLERGSTLRSTGHASPTSSAGSTHLIFAHKQQQQQQQQGPLGESYYHQPDYYSWKQPSTGTGGLKTPREYYNNAGAAASSPQQAHEVFYWTQKPNSGHNGKKKRGAGGVPASPSGSLHSRTAAASQVLFYPSYKKTKPGQPTGYPQYAEALDPPLATGNAAAYYQQQQQLRRQQLHQQQQQQQQQQLSSDEEQAEQHAHLLHLQRRAGSQQQLPAPPPHMAQYQQEFMQRQYRNKHSNCDLGMGDAYYNQGSVGGADGGPVYEEILSNRNSDVQHYEVGDFDVDEVYNNSVGTGVFNNMRAAVAAGGSRYGGGSLSGGSVSSRSQQQQLKKQQQQQSLAQQRSARRCTADDDDDEDEEEDEEATAAEQLHDSVCDEDEEEDESDLEDDAHGLPPQSDERMRRLMAMQDEDFKRRFQRQLRKHGAPLDYGALPPGAGPQPEHNGAVFGVSGGVGEGSLRGAFRQQQQQALNAKSPGGRLAVNELFGHGNSGPPLPPANQTPAQKRQQLQKLSPQSTTSSSSHTSHSNPNPHPHQLTHPHPHQHPPHHQQRHLSAMLDENNTVRCYLEPLAK

Target ORF information:

RefSeq Version	NM_001273878.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Calcium-independent receptor for alpha-latrotoxin (Cirl), transcript variant I, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273878.1

ORF Insert Sequence:

ATGCTACCAA CCATATTGTC AATATCCTAT GAGCATATAT CGTTAGATCT GTCCAAGTAC 
CAAACCGCCT ACGCCTGCGA AGGTAAGAAA CTGACCATCG AGTGCGATCC CGGCGATGTG 
ATCAACCTCA TTCGGGCCAA CTATGGCCGC TTCTCGATTA CCATCTGCAA TGACCACGGG 
AATGTGGAGT GGAGTGTTAA CTGCATGTTT CCCAAGTCAC TCAGCGTACT GAACTCAAGA 
TGTGCCCACA AGCAGAGCTG CGGCGTGTTG GCAGCCACGA GCATGTTCGG GGATCCCTGT 
CCCGGTACCC ACAAGTATCT GGAGGCACAC TACCAGTGCA TAAGTGCAGC CCAAACTTCG 
ACGACGACCA ACAGGCCCAG TCCGCCGCCA TGGGTGCTGA GCAATGGTCC GCCGATCTTT 
GGCAACGGCA GTGGACTGAT CCATCCGCCC GGGGTTGGAG CGGGTGCGCC GCCCCCGCCG 
AGACTTCCCA CACTTCCCGG AGTGGTGGGA ATCAGTGGGA ATCCCGGCCT GTTCAACGTA 
CCACCGCAAC ACACCGCCGT CACGCACTCC ACGCCCTCGA GCAGCACGAC AGCCGTGGGC 
GGTGGACGTT TGAAGGGTGG GGCCACCTCC ACGACGACCA CCAAGCATCC GGCTGGCCGC 
CATGATGGTC TGCCACCGCC GCCGCAACTG CACCACCACC ACAACCACCA CGGTGAAGAC 
ACTGCCTCAC CCACCAAGCC GAGCAGCAAG CTGCCGGCTG GCGGTAATGC CACTTCACCA 
TCCAACACGA GGATACTCAC GGGCGTCGGA GGTTCCGGAA CTGATGACGG AACCCTACTG 
ACCACAAAGA GCTCACCCAA CCGCCCACCG GGCACTGCGG CCAGTGGATC CGTTGTCCCC 
GGGAACGGCA GCGTGGTGCG CACCATCAAC AATATTAATT TGAACGCAGC CGGGATGTCC 
GGAGGCGATG ATGAGTCCAA GTTGTTTTGC GGCCCCACTC ATGCCCGCAA TTTGTACTGG 
AACATGACTC GAGTGGGTGA TGTGAATGTT CAGCCCTGTC CTGGCGGAGC AGCCGGCATC 
GCCAAGTGGC GTTGCGTTCT AATGAAGAGG ATACCCGACT CCGGCTACGA TGAGTACGAT 
GATGACATCA GTTCGACAAC TCCGGCACCC AGCGGTGGCG ACTGTCTGCA CAACAGCAGC 
AGCTGCGAGC CGCCGGTGAG CATGGCCCAC AAGGTAAACC AGCGTCTGCG CAACTTTGAG 
CCCACCTGGC ATCCCGCGAC ACCTGATCTG ACGCAATGCC GCAGCCTTTG GCTCAACAAT 
CTGGAAATGC GAGTAAACCA GCGGGACTCC TCCTTGATCT CCATCGCCAA CGATATGTCC 
GAAGTGACCA GTAGCAAAAC GCTCTACGGC GGCGACATGT TGGTCACCAC GAAGATTATC 
CAAACAGTGT CCGAGAAGAT GATGCACGAC AAGGAGACCT TCCCGGATCA GCGACAGCGC 
GAGGCTATGA TCATGGAGTT GTTGCATTGT GTGGTCAAAA CCGGCTCCAA CCTGCTGGAC 
GAATCGCAGC TGTCCTCGTG GTTGGATCTC AATCCGGAGG ACCAAATGCG TGTAGCCACA 
TCCTTGCTAA CTGGCCTGGA ATACAATGCC TTTCTGCTGG CGGATACGAT CATCAGGGAG 
CGCAGCGTGG TGCAAAAAGT CAAAAATATA TTGCTCTCCG TTCGAGTTCT GGAAACCAAG 
ACTATCCAGT CCAGCGTGGT CTTCCCAGAT TCGGATCAGT GGCCCTTGAG TTCGGATCGT 
ATTGAGCTGC CACGAGCTGC TCTAATAGAT AATAGTGAAG GCGGTCTGGT GCGAATTGTA 
TTCGCCGCCT TCGATCGCCT GGAATCCATT CTAAAGCCCA GCTATGATCA CTTCGATCTC 
AAGAGCTCCC GCAGTTACGT TCGCAATACA GCCATCCTGA GCAACGACAG CGATGTCAAC 
GCGGGGGAGA TCCAACAGCG CCTACGCATC CTGAACAGCA AGGTGATCTC GGCCAGCTTG 
GGCAAGGGGC GTCACATACA ACTCTCCCAG CCCATAACCC TGACACTGAA ACATCTGAAG 
ACCGAGAATG TAACGAATCC CACCTGCGTG TTCTGGAACT ATATTGACCA TGCGTGGTCT 
GCCAACGGAT GCAGTCTGGA GTCCACTAAC CGCACGCACA GCGTCTGCAG TTGCAACCAC 
CTGACAAACT TTGCCATACT AATGGACGTT GTGGATGAGC ACCAGCATTC GTTGTTCACC 
ATGTTCGATG GAAACATGCG CATATTCATC TACATAAGCA TCGGCATCTG CGTGGTCTTC 
ATAGTTATCG CCCTGCTAAC GCTGAAGCTG TTCAATGGGG TCTTTGTGAA GTCCGCGCGC 
ACCTCGATCT ATACCAGCAT TTACCTTTGC CTCCTGGCCA TCGAGCTGCT CTTTCTCCTG 
GGCATTGAAC AGACCGAAAC AAGCATTTTC TGCGGCTTCA TTACTATTTT CCTACACTGT 
GCCATCCTAT CGGGCACCGC CTGGTTCTGT TACGAAGCCT TCCATTCGTA CTCAACGCTC 
ACCTCGGACG AGCTCCTGCT GGAGGTGGAC CAGACGCCCA AGGTGAACTG CTACTACCTC 
TTGTCCTACG GACTGTCGCT GAGCGTGGTG GCCATCTCGC TGGTCATCGA TCCCAGCACC 
TATACCCAAA ACGATTATTG CGTGCTGATG GAGGCGAATG CCTTGTTTTA TGCCACCTTT 
GTAATACCAG TGCTTGTCTT CTTTGTGGCT GCCATTGGTT ACACATTCCT CTCCTGGATT 
ATAATGTGCC GCAAAAGTCG CACGGGTCTA AAGACCAAGG AACATACTCG CCTCGCTAGC 
GTGCGGTTCG ACATACGCTG CTCCTTTGTG TTCCTCTTGC TGCTCAGCGC TGTTTGGTGC 
TCGGCCTACT TCTATTTGCG AGGAGCCAAA ATGGACGATG ACACGGCTGA TGTGTATGGA 
TACTGCTTCA TCTGCTTCAA CACATTGCTG GGGCTCTATA TCTTCGTGTT CCATTGCATT 
CAAAACGAAA AGATCCGGCG GGAGTATCGG AAGTATGTGA GACAGCACGC TTGGCTGCCC 
AAGTGCTTGC GCTGCTCGAA AACATCAATT TCCTCGGGCA TTGTTACCGG CAATGGACCC 
ACAGCCGGAA CCCTTTGCAG CGTCTCCACG TCCAAGAAGC CCAAGCTGCC GTTAGGAGTG 
AGCGAAGAGG CGCATGACGA TCCCCAGCAG CAACAGCAGA CACCAGTGCC CATCACAGAG 
GATGCCATTA TGGGAGCCAC CTCTGATTGT GAACTGAACG AGGCCCAGCA AAGAAGAACC 
CTAAAAAGTG GCCTAATGAC GGGCACACTA CAGGCTCCAC CGCAGACCCT TGGTGGCCAT 
GTTGTGCTCG AAAGAGGTAG CACTCTCCGC TCCACTGGTC ATGCCTCACC CACCAGCTCT 
GCCGGGTCCA CACACCTGAT TTTTGCGCAC AAGCAACAAC AACAACAGCA GCAACAGGGA 
CCTTTGGGCG AGTCTTACTA CCATCAGCCG GACTACTACA GCTGGAAGCA ACCATCAACT 
GGAACAGGAG GATTGAAAAC ACCGCGGGAG TACTACAATA ATGCGGGTGC TGCTGCATCA 
TCGCCGCAAC AGGCGCACGA GGTATTCTAC TGGACTCAAA AGCCGAACAG CGGCCACAAT 
GGCAAAAAGA AGAGGGGCGC CGGAGGAGTT CCCGCCTCGC CTAGTGGATC TCTGCACAGT 
CGCACGGCCG CCGCCTCCCA GGTGCTTTTC TATCCATCGT ACAAGAAGAC CAAGCCTGGC 
CAGCCAACAG GCTATCCGCA ATACGCGGAG GCGTTGGACC CACCACTAGC CACTGGCAAT 
GCGGCTGCCT ACTACCAGCA GCAGCAACAG TTGCGTCGCC AGCAGCTACA TCAGCAGCAG 
CAACAGCAGC AGCAGCAGCA ACTCTCCTCG GACGAGGAGC AGGCCGAGCA ACATGCTCAC 
CTGTTGCACC TGCAACGACG AGCTGGTAGC CAGCAGCAGC TCCCTGCTCC ACCGCCACAC 
ATGGCGCAGT ACCAGCAGGA GTTTATGCAG CGCCAGTATA GAAATAAGCA TTCCAACTGT 
GATCTGGGCA TGGGCGATGC CTACTACAAC CAAGGCAGCG TCGGCGGCGC GGATGGTGGG 
CCGGTCTACG AGGAGATCCT CAGCAACCGC AACTCGGATG TGCAGCATTA CGAGGTGGGT 
GACTTCGATG TGGACGAGGT GTACAACAAT AGCGTTGGCA CTGGCGTCTT CAACAACATG 
AGAGCGGCGG TGGCCGCCGG CGGTAGTCGG TACGGCGGCG GAAGCCTGAG TGGCGGCAGT 
GTCTCGTCCA GGAGCCAACA GCAGCAGCTC AAGAAGCAGC AGCAACAACA GTCGCTGGCT 
CAGCAAAGAT CGGCTCGACG CTGCACGGCG GATGACGATG ACGACGAAGA CGAGGAGGAG 
GATGAGGAGG CAACGGCCGC GGAGCAATTG CACGACAGCG TCTGTGATGA GGATGAGGAG 
GAGGACGAGA GCGACTTGGA GGATGATGCT CATGGATTGC CACCGCAGAG CGATGAGCGG 
ATGCGTCGTC TGATGGCGAT GCAGGACGAG GATTTTAAGC GGCGGTTTCA ACGTCAGCTG 
CGCAAACATG GAGCGCCTCT TGACTACGGG GCTTTGCCAC CAGGAGCAGG ACCGCAACCC 
GAGCACAACG GTGCGGTTTT TGGGGTTAGC GGCGGCGTTG GTGAGGGCTC CCTGCGTGGC 
GCATTCCGGC AGCAGCAGCA GCAAGCACTG AATGCCAAGT CGCCAGGCGG CCGTTTGGCG 
GTAAATGAGC TATTCGGTCA CGGCAACTCG GGACCACCGC TGCCGCCGGC GAATCAGACG 
CCCGCGCAGA AGCGCCAGCA GCTACAGAAA CTGTCACCAC AGTCCACCAC ATCATCGTCG 
TCGCATACAT CACACAGCAA TCCCAATCCG CATCCCCACC AGCTAACCCA TCCGCATCCC 
CATCAGCATC CTCCGCACCA TCAGCAGCGC CACCTGTCGG CCATGCTCGA TGAGAACAAC 
ACGGTGCGAT GTTATCTGGA ACCACTGGCT AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cirl ( NM_001273880.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260809.1 Drosophila melanogaster Calcium-independent receptor for alpha-latrotoxin (Cirl), transcript variant K, mRNA.

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CloneID	OFa09298
Clone ID Related Accession (Same CDS sequence)	NM_001273880.2 , NM_001259275.2
Accession Version	NM_001273880.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2799bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Calcium-independent receptor for alpha-latrotoxin, isoform K
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001273880.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTCCCA AGTCACTCAG CGTACTGAAC TCAAGATGTG CCCACAAGCA GAGCTGCGGC 
GTGTTGGCAG CCACGAGCAT GTTCGGGGAT CCCTGTCCCG GTACCCACAA GTATCTGGAG 
GCACACTACC AGTGCATAAG TGCAGCCCAA ACTTCGACGA CGACCAACAG GCCCAGTCCG 
CCGCCATGGG TGCTGAGCAA TGGTCCGCCG ATCTTTGGCA ACGGCAGTGG ACTGATCCAT 
CCGCCCGGGG TTGGAGCGGG TGCGCCGCCC CCGCCGAGAC TTCCCACACT TCCCGGAGTG 
GTGGGAATCA GTGGGAATCC CGGCCTGTTC AACGTACCAC CGCAACACAC CGCCGTCACG 
CACTCCACGC CCTCGAGCAG CACGACAGCC GTGGGCGGTG GACGTTTGAA GGGTGGGGCC 
ACCTCCACGA CGACCACCAA GCATCCGGCT GGCCGCCATG ATGGTCTGCC ACCGCCGCCG 
CAACTGCACC ACCACCACAA CCACCACGGT GAAGACACTG CCTCACCCAC CAAGCCGAGC 
AGCAAGCTGC CGGCTGGCGG TAATGCCACT TCACCATCCA ACACGAGGAT ACTCACGGGC 
GTCGGAGGTT CCGGAACTGA TGACGGAACC CTACTGACCA CAAAGAGCTC ACCCAACCGC 
CCACCGGGCA CTGCGGCCAG TGGATCCGTT GTCCCCGGGA ACGGCAGCGT GGTGCGCACC 
ATCAACAATA TTAATTTGAA CGCAGCCGGG ATGTCCGGAG GCGATGATGA GTCCAAGTTG 
TTTTGCGGCC CCACTCATGC CCGCAATTTG TACTGGAACA TGACTCGAGT GGGTGATGTG 
AATGTTCAGC CCTGTCCTGG CGGAGCAGCC GGCATCGCCA AGTGGCGTTG CGTTCTAATG 
AAGAGGATAC CCGACTCCGG CTACGATGAG TACGATGATG ACATCAGTTC GACAACTCCG 
GCACCCAGCG GTGGCGACTG TCTGCACAAC AGCAGCAGCT GCGAGCCGCC GGTGAGCATG 
GCCCACAAGG TAAACCAGCG TCTGCGCAAC TTTGAGCCCA CCTGGCATCC CGCGACACCT 
GATCTGACGC AATGCCGCAG CCTTTGGCTC AACAATCTGG AAATGCGAGT AAACCAGCGG 
GACTCCTCCT TGATCTCCAT CGCCAACGAT ATGTCCGAAG TGACCAGTAG CAAAACGCTC 
TACGGCGGCG ACATGTTGGT CACCACGAAG ATTATCCAAA CAGTGTCCGA GAAGATGATG 
CACGACAAGG AGACCTTCCC GGATCAGCGA CAGCGCGAGG CTATGATCAT GGAGTTGTTG 
CATTGTGTGG TCAAAACCGG CTCCAACCTG CTGGACGAAT CGCAGCTGTC CTCGTGGTTG 
GATCTCAATC CGGAGGACCA AATGCGTGTA GCCACATCCT TGCTAACTGG CCTGGAATAC 
AATGCCTTTC TGCTGGCGGA TACGATCATC AGGGAGCGCA GCGTGGTGCA AAAAGTCAAA 
AATATATTGC TCTCCGTTCG AGTTCTGGAA ACCAAGACTA TCCAGTCCAG CGTGGTCTTC 
CCAGATTCGG ATCAGTGGCC CTTGAGTTCG GATCGTATTG AGCTGCCACG AGCTGCTCTA 
ATAGATAATA GTGAAGGCGG TCTGGTGCGA ATTGTATTCG CCGCCTTCGA TCGCCTGGAA 
TCCATTCTAA AGCCCAGCTA TGATCACTTC GATCTCAAGA GCTCCCGCAG TTACGCCATC 
CTGAGCAACG ACAGCGATGT CAACGCGGGG GAGATCCAAC AGCGCCTACG CATCCTGAAC 
AGCAAGGTGA TCTCGGCCAG CTTGGGCAAG GGGCGTCACA TACAACTCTC CCAGCCCATA 
ACCCTGACAC TGAAACATCT GAAGACCGAG AATGTAACGA ATCCCACCTG CGTGTTCTGG 
AACTATATTG ACCATGCGTG GTCTGCCAAC GGATGCAGTC TGGAGTCCAC TAACCGCACG 
CACAGCGTCT GCAGTTGCAA CCACCTGACA AACTTTGCCA TACTAATGGA CGTTGTGGAT 
GAGCACCAGC ATTCGTTGTT CACCATGTTC GATGGAAACA TGCGCATATT CATCTACATA 
AGCATCGGCA TCTGCGTGGT CTTCATAGTT ATCGCCCTGC TAACGCTGAA GCTGTTCAAT 
GGGGTCTTTG TGAAGGTAAG AAACGGCTCC AATCCCTTGC CGCATCAGCG GTCGGGCAGC 
AGACGCCAGC AAAACAATAT TCGCGACCAG ACCCACGAGT CCTTGACCCT GACCACGCCA 
ACCAGTCAGT CCAATGTGCC GCCGCCCAGT CATGGGAACA CGAACTTTAT CCAACACAAT 
TCCATCCGCA ACTCACACCG CAACAATCTG AACTACAATG TCCAACAGCA GCAGCAGCAA 
CAGCAACAAC AAGTTGTTGC GGCAGCTGCT GCTGCTGTTG TGCTGTCCAA TCAGCCGCAG 
CGAAATATGC AACATGCCAT GAACAACTTG AATCTGAATC TGCATCAGCA CGGTCAGCAG 
ACGGCTGCTG CTGCAGCTGC TGCTGCCTCA ATAGCCGCTG CACTGCAGCA ACATGCAGTT 
CAGGCCAGCA ACGCCAGCAA TAACCTCAAC ATCAGCCACA ACTATCTGCA GCAGCAGCAT 
GTCCAGCAGC AGCAGCAGCA GCAGCGTGGC CAGCAGCCGC AGCCACATCC GCACCGCAAT 
CACAACCTTA ATGTGGACGG CAATGGATTG GATAACACGA ACAACATCAT TATGCAGGCC 
AACATGGACG ACTTACAATA TAAGTGGTGG TGGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260809.1
CDS	869..3667
Translation	MFPKSLSVLNSRCAHKQSCGVLAATSMFGDPCPGTHKYLEAHYQCISAAQTSTTTNRPSPPPWVLSNGPPIFGNGSGLIHPPGVGAGAPPPPRLPTLPGVVGISGNPGLFNVPPQHTAVTHSTPSSSTTAVGGGRLKGGATSTTTTKHPAGRHDGLPPPPQLHHHHNHHGEDTASPTKPSSKLPAGGNATSPSNTRILTGVGGSGTDDGTLLTTKSSPNRPPGTAASGSVVPGNGSVVRTINNINLNAAGMSGGDDESKLFCGPTHARNLYWNMTRVGDVNVQPCPGGAAGIAKWRCVLMKRIPDSGYDEYDDDISSTTPAPSGGDCLHNSSSCEPPVSMAHKVNQRLRNFEPTWHPATPDLTQCRSLWLNNLEMRVNQRDSSLISIANDMSEVTSSKTLYGGDMLVTTKIIQTVSEKMMHDKETFPDQRQREAMIMELLHCVVKTGSNLLDESQLSSWLDLNPEDQMRVATSLLTGLEYNAFLLADTIIRERSVVQKVKNILLSVRVLETKTIQSSVVFPDSDQWPLSSDRIELPRAALIDNSEGGLVRIVFAAFDRLESILKPSYDHFDLKSSRSYAILSNDSDVNAGEIQQRLRILNSKVISASLGKGRHIQLSQPITLTLKHLKTENVTNPTCVFWNYIDHAWSANGCSLESTNRTHSVCSCNHLTNFAILMDVVDEHQHSLFTMFDGNMRIFIYISIGICVVFIVIALLTLKLFNGVFVKVRNGSNPLPHQRSGSRRQQNNIRDQTHESLTLTTPTSQSNVPPPSHGNTNFIQHNSIRNSHRNNLNYNVQQQQQQQQQQVVAAAAAAVVLSNQPQRNMQHAMNNLNLNLHQHGQQTAAAAAAAASIAAALQQHAVQASNASNNLNISHNYLQQQHVQQQQQQQRGQQPQPHPHRNHNLNVDGNGLDNTNNIIMQANMDDLQYKWWWW

Target ORF information:

RefSeq Version	NM_001273880.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Calcium-independent receptor for alpha-latrotoxin (Cirl), transcript variant K, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273880.2

ORF Insert Sequence:

ATGTTTCCCA AGTCACTCAG CGTACTGAAC TCAAGATGTG CCCACAAGCA GAGCTGCGGC 
GTGTTGGCAG CCACGAGCAT GTTCGGGGAT CCCTGTCCCG GTACCCACAA GTATCTGGAG 
GCACACTACC AGTGCATAAG TGCAGCCCAA ACTTCGACGA CGACCAACAG GCCCAGTCCG 
CCGCCATGGG TGCTGAGCAA TGGTCCGCCG ATCTTTGGCA ACGGCAGTGG ACTGATCCAT 
CCGCCCGGGG TTGGAGCGGG TGCGCCGCCC CCGCCGAGAC TTCCCACACT TCCCGGAGTG 
GTGGGAATCA GTGGGAATCC CGGCCTGTTC AACGTACCAC CGCAACACAC CGCCGTCACG 
CACTCCACGC CCTCGAGCAG CACGACAGCC GTGGGCGGTG GACGTTTGAA GGGTGGGGCC 
ACCTCCACGA CGACCACCAA GCATCCGGCT GGCCGCCATG ATGGTCTGCC ACCGCCGCCG 
CAACTGCACC ACCACCACAA CCACCACGGT GAAGACACTG CCTCACCCAC CAAGCCGAGC 
AGCAAGCTGC CGGCTGGCGG TAATGCCACT TCACCATCCA ACACGAGGAT ACTCACGGGC 
GTCGGAGGTT CCGGAACTGA TGACGGAACC CTACTGACCA CAAAGAGCTC ACCCAACCGC 
CCACCGGGCA CTGCGGCCAG TGGATCCGTT GTCCCCGGGA ACGGCAGCGT GGTGCGCACC 
ATCAACAATA TTAATTTGAA CGCAGCCGGG ATGTCCGGAG GCGATGATGA GTCCAAGTTG 
TTTTGCGGCC CCACTCATGC CCGCAATTTG TACTGGAACA TGACTCGAGT GGGTGATGTG 
AATGTTCAGC CCTGTCCTGG CGGAGCAGCC GGCATCGCCA AGTGGCGTTG CGTTCTAATG 
AAGAGGATAC CCGACTCCGG CTACGATGAG TACGATGATG ACATCAGTTC GACAACTCCG 
GCACCCAGCG GTGGCGACTG TCTGCACAAC AGCAGCAGCT GCGAGCCGCC GGTGAGCATG 
GCCCACAAGG TAAACCAGCG TCTGCGCAAC TTTGAGCCCA CCTGGCATCC CGCGACACCT 
GATCTGACGC AATGCCGCAG CCTTTGGCTC AACAATCTGG AAATGCGAGT AAACCAGCGG 
GACTCCTCCT TGATCTCCAT CGCCAACGAT ATGTCCGAAG TGACCAGTAG CAAAACGCTC 
TACGGCGGCG ACATGTTGGT CACCACGAAG ATTATCCAAA CAGTGTCCGA GAAGATGATG 
CACGACAAGG AGACCTTCCC GGATCAGCGA CAGCGCGAGG CTATGATCAT GGAGTTGTTG 
CATTGTGTGG TCAAAACCGG CTCCAACCTG CTGGACGAAT CGCAGCTGTC CTCGTGGTTG 
GATCTCAATC CGGAGGACCA AATGCGTGTA GCCACATCCT TGCTAACTGG CCTGGAATAC 
AATGCCTTTC TGCTGGCGGA TACGATCATC AGGGAGCGCA GCGTGGTGCA AAAAGTCAAA 
AATATATTGC TCTCCGTTCG AGTTCTGGAA ACCAAGACTA TCCAGTCCAG CGTGGTCTTC 
CCAGATTCGG ATCAGTGGCC CTTGAGTTCG GATCGTATTG AGCTGCCACG AGCTGCTCTA 
ATAGATAATA GTGAAGGCGG TCTGGTGCGA ATTGTATTCG CCGCCTTCGA TCGCCTGGAA 
TCCATTCTAA AGCCCAGCTA TGATCACTTC GATCTCAAGA GCTCCCGCAG TTACGCCATC 
CTGAGCAACG ACAGCGATGT CAACGCGGGG GAGATCCAAC AGCGCCTACG CATCCTGAAC 
AGCAAGGTGA TCTCGGCCAG CTTGGGCAAG GGGCGTCACA TACAACTCTC CCAGCCCATA 
ACCCTGACAC TGAAACATCT GAAGACCGAG AATGTAACGA ATCCCACCTG CGTGTTCTGG 
AACTATATTG ACCATGCGTG GTCTGCCAAC GGATGCAGTC TGGAGTCCAC TAACCGCACG 
CACAGCGTCT GCAGTTGCAA CCACCTGACA AACTTTGCCA TACTAATGGA CGTTGTGGAT 
GAGCACCAGC ATTCGTTGTT CACCATGTTC GATGGAAACA TGCGCATATT CATCTACATA 
AGCATCGGCA TCTGCGTGGT CTTCATAGTT ATCGCCCTGC TAACGCTGAA GCTGTTCAAT 
GGGGTCTTTG TGAAGGTAAG AAACGGCTCC AATCCCTTGC CGCATCAGCG GTCGGGCAGC 
AGACGCCAGC AAAACAATAT TCGCGACCAG ACCCACGAGT CCTTGACCCT GACCACGCCA 
ACCAGTCAGT CCAATGTGCC GCCGCCCAGT CATGGGAACA CGAACTTTAT CCAACACAAT 
TCCATCCGCA ACTCACACCG CAACAATCTG AACTACAATG TCCAACAGCA GCAGCAGCAA 
CAGCAACAAC AAGTTGTTGC GGCAGCTGCT GCTGCTGTTG TGCTGTCCAA TCAGCCGCAG 
CGAAATATGC AACATGCCAT GAACAACTTG AATCTGAATC TGCATCAGCA CGGTCAGCAG 
ACGGCTGCTG CTGCAGCTGC TGCTGCCTCA ATAGCCGCTG CACTGCAGCA ACATGCAGTT 
CAGGCCAGCA ACGCCAGCAA TAACCTCAAC ATCAGCCACA ACTATCTGCA GCAGCAGCAT 
GTCCAGCAGC AGCAGCAGCA GCAGCGTGGC CAGCAGCCGC AGCCACATCC GCACCGCAAT 
CACAACCTTA ATGTGGACGG CAATGGATTG GATAACACGA ACAACATCAT TATGCAGGCC 
AACATGGACG ACTTACAATA TAAGTGGTGG TGGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cirl ( NM_001259275.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246204.1 Drosophila melanogaster Calcium-independent receptor for alpha-latrotoxin (Cirl), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa09298
Clone ID Related Accession (Same CDS sequence)	NM_001273880.2 , NM_001259275.2
Accession Version	NM_001259275.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2799bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Calcium-independent receptor for alpha-latrotoxin, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001259275.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTCCCA AGTCACTCAG CGTACTGAAC TCAAGATGTG CCCACAAGCA GAGCTGCGGC 
GTGTTGGCAG CCACGAGCAT GTTCGGGGAT CCCTGTCCCG GTACCCACAA GTATCTGGAG 
GCACACTACC AGTGCATAAG TGCAGCCCAA ACTTCGACGA CGACCAACAG GCCCAGTCCG 
CCGCCATGGG TGCTGAGCAA TGGTCCGCCG ATCTTTGGCA ACGGCAGTGG ACTGATCCAT 
CCGCCCGGGG TTGGAGCGGG TGCGCCGCCC CCGCCGAGAC TTCCCACACT TCCCGGAGTG 
GTGGGAATCA GTGGGAATCC CGGCCTGTTC AACGTACCAC CGCAACACAC CGCCGTCACG 
CACTCCACGC CCTCGAGCAG CACGACAGCC GTGGGCGGTG GACGTTTGAA GGGTGGGGCC 
ACCTCCACGA CGACCACCAA GCATCCGGCT GGCCGCCATG ATGGTCTGCC ACCGCCGCCG 
CAACTGCACC ACCACCACAA CCACCACGGT GAAGACACTG CCTCACCCAC CAAGCCGAGC 
AGCAAGCTGC CGGCTGGCGG TAATGCCACT TCACCATCCA ACACGAGGAT ACTCACGGGC 
GTCGGAGGTT CCGGAACTGA TGACGGAACC CTACTGACCA CAAAGAGCTC ACCCAACCGC 
CCACCGGGCA CTGCGGCCAG TGGATCCGTT GTCCCCGGGA ACGGCAGCGT GGTGCGCACC 
ATCAACAATA TTAATTTGAA CGCAGCCGGG ATGTCCGGAG GCGATGATGA GTCCAAGTTG 
TTTTGCGGCC CCACTCATGC CCGCAATTTG TACTGGAACA TGACTCGAGT GGGTGATGTG 
AATGTTCAGC CCTGTCCTGG CGGAGCAGCC GGCATCGCCA AGTGGCGTTG CGTTCTAATG 
AAGAGGATAC CCGACTCCGG CTACGATGAG TACGATGATG ACATCAGTTC GACAACTCCG 
GCACCCAGCG GTGGCGACTG TCTGCACAAC AGCAGCAGCT GCGAGCCGCC GGTGAGCATG 
GCCCACAAGG TAAACCAGCG TCTGCGCAAC TTTGAGCCCA CCTGGCATCC CGCGACACCT 
GATCTGACGC AATGCCGCAG CCTTTGGCTC AACAATCTGG AAATGCGAGT AAACCAGCGG 
GACTCCTCCT TGATCTCCAT CGCCAACGAT ATGTCCGAAG TGACCAGTAG CAAAACGCTC 
TACGGCGGCG ACATGTTGGT CACCACGAAG ATTATCCAAA CAGTGTCCGA GAAGATGATG 
CACGACAAGG AGACCTTCCC GGATCAGCGA CAGCGCGAGG CTATGATCAT GGAGTTGTTG 
CATTGTGTGG TCAAAACCGG CTCCAACCTG CTGGACGAAT CGCAGCTGTC CTCGTGGTTG 
GATCTCAATC CGGAGGACCA AATGCGTGTA GCCACATCCT TGCTAACTGG CCTGGAATAC 
AATGCCTTTC TGCTGGCGGA TACGATCATC AGGGAGCGCA GCGTGGTGCA AAAAGTCAAA 
AATATATTGC TCTCCGTTCG AGTTCTGGAA ACCAAGACTA TCCAGTCCAG CGTGGTCTTC 
CCAGATTCGG ATCAGTGGCC CTTGAGTTCG GATCGTATTG AGCTGCCACG AGCTGCTCTA 
ATAGATAATA GTGAAGGCGG TCTGGTGCGA ATTGTATTCG CCGCCTTCGA TCGCCTGGAA 
TCCATTCTAA AGCCCAGCTA TGATCACTTC GATCTCAAGA GCTCCCGCAG TTACGCCATC 
CTGAGCAACG ACAGCGATGT CAACGCGGGG GAGATCCAAC AGCGCCTACG CATCCTGAAC 
AGCAAGGTGA TCTCGGCCAG CTTGGGCAAG GGGCGTCACA TACAACTCTC CCAGCCCATA 
ACCCTGACAC TGAAACATCT GAAGACCGAG AATGTAACGA ATCCCACCTG CGTGTTCTGG 
AACTATATTG ACCATGCGTG GTCTGCCAAC GGATGCAGTC TGGAGTCCAC TAACCGCACG 
CACAGCGTCT GCAGTTGCAA CCACCTGACA AACTTTGCCA TACTAATGGA CGTTGTGGAT 
GAGCACCAGC ATTCGTTGTT CACCATGTTC GATGGAAACA TGCGCATATT CATCTACATA 
AGCATCGGCA TCTGCGTGGT CTTCATAGTT ATCGCCCTGC TAACGCTGAA GCTGTTCAAT 
GGGGTCTTTG TGAAGGTAAG AAACGGCTCC AATCCCTTGC CGCATCAGCG GTCGGGCAGC 
AGACGCCAGC AAAACAATAT TCGCGACCAG ACCCACGAGT CCTTGACCCT GACCACGCCA 
ACCAGTCAGT CCAATGTGCC GCCGCCCAGT CATGGGAACA CGAACTTTAT CCAACACAAT 
TCCATCCGCA ACTCACACCG CAACAATCTG AACTACAATG TCCAACAGCA GCAGCAGCAA 
CAGCAACAAC AAGTTGTTGC GGCAGCTGCT GCTGCTGTTG TGCTGTCCAA TCAGCCGCAG 
CGAAATATGC AACATGCCAT GAACAACTTG AATCTGAATC TGCATCAGCA CGGTCAGCAG 
ACGGCTGCTG CTGCAGCTGC TGCTGCCTCA ATAGCCGCTG CACTGCAGCA ACATGCAGTT 
CAGGCCAGCA ACGCCAGCAA TAACCTCAAC ATCAGCCACA ACTATCTGCA GCAGCAGCAT 
GTCCAGCAGC AGCAGCAGCA GCAGCGTGGC CAGCAGCCGC AGCCACATCC GCACCGCAAT 
CACAACCTTA ATGTGGACGG CAATGGATTG GATAACACGA ACAACATCAT TATGCAGGCC 
AACATGGACG ACTTACAATA TAAGTGGTGG TGGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246204.1
CDS	313..3111
Translation	MFPKSLSVLNSRCAHKQSCGVLAATSMFGDPCPGTHKYLEAHYQCISAAQTSTTTNRPSPPPWVLSNGPPIFGNGSGLIHPPGVGAGAPPPPRLPTLPGVVGISGNPGLFNVPPQHTAVTHSTPSSSTTAVGGGRLKGGATSTTTTKHPAGRHDGLPPPPQLHHHHNHHGEDTASPTKPSSKLPAGGNATSPSNTRILTGVGGSGTDDGTLLTTKSSPNRPPGTAASGSVVPGNGSVVRTINNINLNAAGMSGGDDESKLFCGPTHARNLYWNMTRVGDVNVQPCPGGAAGIAKWRCVLMKRIPDSGYDEYDDDISSTTPAPSGGDCLHNSSSCEPPVSMAHKVNQRLRNFEPTWHPATPDLTQCRSLWLNNLEMRVNQRDSSLISIANDMSEVTSSKTLYGGDMLVTTKIIQTVSEKMMHDKETFPDQRQREAMIMELLHCVVKTGSNLLDESQLSSWLDLNPEDQMRVATSLLTGLEYNAFLLADTIIRERSVVQKVKNILLSVRVLETKTIQSSVVFPDSDQWPLSSDRIELPRAALIDNSEGGLVRIVFAAFDRLESILKPSYDHFDLKSSRSYAILSNDSDVNAGEIQQRLRILNSKVISASLGKGRHIQLSQPITLTLKHLKTENVTNPTCVFWNYIDHAWSANGCSLESTNRTHSVCSCNHLTNFAILMDVVDEHQHSLFTMFDGNMRIFIYISIGICVVFIVIALLTLKLFNGVFVKVRNGSNPLPHQRSGSRRQQNNIRDQTHESLTLTTPTSQSNVPPPSHGNTNFIQHNSIRNSHRNNLNYNVQQQQQQQQQQVVAAAAAAVVLSNQPQRNMQHAMNNLNLNLHQHGQQTAAAAAAAASIAAALQQHAVQASNASNNLNISHNYLQQQHVQQQQQQQRGQQPQPHPHRNHNLNVDGNGLDNTNNIIMQANMDDLQYKWWWW

Target ORF information:

RefSeq Version	NM_001259275.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Calcium-independent receptor for alpha-latrotoxin (Cirl), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259275.2

ORF Insert Sequence:

ATGTTTCCCA AGTCACTCAG CGTACTGAAC TCAAGATGTG CCCACAAGCA GAGCTGCGGC 
GTGTTGGCAG CCACGAGCAT GTTCGGGGAT CCCTGTCCCG GTACCCACAA GTATCTGGAG 
GCACACTACC AGTGCATAAG TGCAGCCCAA ACTTCGACGA CGACCAACAG GCCCAGTCCG 
CCGCCATGGG TGCTGAGCAA TGGTCCGCCG ATCTTTGGCA ACGGCAGTGG ACTGATCCAT 
CCGCCCGGGG TTGGAGCGGG TGCGCCGCCC CCGCCGAGAC TTCCCACACT TCCCGGAGTG 
GTGGGAATCA GTGGGAATCC CGGCCTGTTC AACGTACCAC CGCAACACAC CGCCGTCACG 
CACTCCACGC CCTCGAGCAG CACGACAGCC GTGGGCGGTG GACGTTTGAA GGGTGGGGCC 
ACCTCCACGA CGACCACCAA GCATCCGGCT GGCCGCCATG ATGGTCTGCC ACCGCCGCCG 
CAACTGCACC ACCACCACAA CCACCACGGT GAAGACACTG CCTCACCCAC CAAGCCGAGC 
AGCAAGCTGC CGGCTGGCGG TAATGCCACT TCACCATCCA ACACGAGGAT ACTCACGGGC 
GTCGGAGGTT CCGGAACTGA TGACGGAACC CTACTGACCA CAAAGAGCTC ACCCAACCGC 
CCACCGGGCA CTGCGGCCAG TGGATCCGTT GTCCCCGGGA ACGGCAGCGT GGTGCGCACC 
ATCAACAATA TTAATTTGAA CGCAGCCGGG ATGTCCGGAG GCGATGATGA GTCCAAGTTG 
TTTTGCGGCC CCACTCATGC CCGCAATTTG TACTGGAACA TGACTCGAGT GGGTGATGTG 
AATGTTCAGC CCTGTCCTGG CGGAGCAGCC GGCATCGCCA AGTGGCGTTG CGTTCTAATG 
AAGAGGATAC CCGACTCCGG CTACGATGAG TACGATGATG ACATCAGTTC GACAACTCCG 
GCACCCAGCG GTGGCGACTG TCTGCACAAC AGCAGCAGCT GCGAGCCGCC GGTGAGCATG 
GCCCACAAGG TAAACCAGCG TCTGCGCAAC TTTGAGCCCA CCTGGCATCC CGCGACACCT 
GATCTGACGC AATGCCGCAG CCTTTGGCTC AACAATCTGG AAATGCGAGT AAACCAGCGG 
GACTCCTCCT TGATCTCCAT CGCCAACGAT ATGTCCGAAG TGACCAGTAG CAAAACGCTC 
TACGGCGGCG ACATGTTGGT CACCACGAAG ATTATCCAAA CAGTGTCCGA GAAGATGATG 
CACGACAAGG AGACCTTCCC GGATCAGCGA CAGCGCGAGG CTATGATCAT GGAGTTGTTG 
CATTGTGTGG TCAAAACCGG CTCCAACCTG CTGGACGAAT CGCAGCTGTC CTCGTGGTTG 
GATCTCAATC CGGAGGACCA AATGCGTGTA GCCACATCCT TGCTAACTGG CCTGGAATAC 
AATGCCTTTC TGCTGGCGGA TACGATCATC AGGGAGCGCA GCGTGGTGCA AAAAGTCAAA 
AATATATTGC TCTCCGTTCG AGTTCTGGAA ACCAAGACTA TCCAGTCCAG CGTGGTCTTC 
CCAGATTCGG ATCAGTGGCC CTTGAGTTCG GATCGTATTG AGCTGCCACG AGCTGCTCTA 
ATAGATAATA GTGAAGGCGG TCTGGTGCGA ATTGTATTCG CCGCCTTCGA TCGCCTGGAA 
TCCATTCTAA AGCCCAGCTA TGATCACTTC GATCTCAAGA GCTCCCGCAG TTACGCCATC 
CTGAGCAACG ACAGCGATGT CAACGCGGGG GAGATCCAAC AGCGCCTACG CATCCTGAAC 
AGCAAGGTGA TCTCGGCCAG CTTGGGCAAG GGGCGTCACA TACAACTCTC CCAGCCCATA 
ACCCTGACAC TGAAACATCT GAAGACCGAG AATGTAACGA ATCCCACCTG CGTGTTCTGG 
AACTATATTG ACCATGCGTG GTCTGCCAAC GGATGCAGTC TGGAGTCCAC TAACCGCACG 
CACAGCGTCT GCAGTTGCAA CCACCTGACA AACTTTGCCA TACTAATGGA CGTTGTGGAT 
GAGCACCAGC ATTCGTTGTT CACCATGTTC GATGGAAACA TGCGCATATT CATCTACATA 
AGCATCGGCA TCTGCGTGGT CTTCATAGTT ATCGCCCTGC TAACGCTGAA GCTGTTCAAT 
GGGGTCTTTG TGAAGGTAAG AAACGGCTCC AATCCCTTGC CGCATCAGCG GTCGGGCAGC 
AGACGCCAGC AAAACAATAT TCGCGACCAG ACCCACGAGT CCTTGACCCT GACCACGCCA 
ACCAGTCAGT CCAATGTGCC GCCGCCCAGT CATGGGAACA CGAACTTTAT CCAACACAAT 
TCCATCCGCA ACTCACACCG CAACAATCTG AACTACAATG TCCAACAGCA GCAGCAGCAA 
CAGCAACAAC AAGTTGTTGC GGCAGCTGCT GCTGCTGTTG TGCTGTCCAA TCAGCCGCAG 
CGAAATATGC AACATGCCAT GAACAACTTG AATCTGAATC TGCATCAGCA CGGTCAGCAG 
ACGGCTGCTG CTGCAGCTGC TGCTGCCTCA ATAGCCGCTG CACTGCAGCA ACATGCAGTT 
CAGGCCAGCA ACGCCAGCAA TAACCTCAAC ATCAGCCACA ACTATCTGCA GCAGCAGCAT 
GTCCAGCAGC AGCAGCAGCA GCAGCGTGGC CAGCAGCCGC AGCCACATCC GCACCGCAAT 
CACAACCTTA ATGTGGACGG CAATGGATTG GATAACACGA ACAACATCAT TATGCAGGCC 
AACATGGACG ACTTACAATA TAAGTGGTGG TGGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cirl ( NM_001273879.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260808.1 Drosophila melanogaster Calcium-independent receptor for alpha-latrotoxin (Cirl), transcript variant J, mRNA.

Price & Availability↑

CloneID	OFa09299
Clone ID Related Accession (Same CDS sequence)	NM_001273879.2
Accession Version	NM_001273879.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3003bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Calcium-independent receptor for alpha-latrotoxin, isoform J
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001273879.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTACCAA CCATATTGTC AATATCCTAT GAGCATATAT CGTTAGATCT GTCCAAGTAC 
CAAACCGCCT ACGCCTGCGA AGGTAAGAAA CTGACCATCG AGTGCGATCC CGGCGATGTG 
ATCAACCTCA TTCGGGCCAA CTATGGCCGC TTCTCGATTA CCATCTGCAA TGACCACGGG 
AATGTGGAGT GGAGTGTTAA CTGCATGTTT CCCAAGTCAC TCAGCGTACT GAACTCAAGA 
TGTGCCCACA AGCAGAGCTG CGGCGTGTTG GCAGCCACGA GCATGTTCGG GGATCCCTGT 
CCCGGTACCC ACAAGTATCT GGAGGCACAC TACCAGTGCA TAAGTGCAGC CCAAACTTCG 
ACGACGACCA ACAGGCCCAG TCCGCCGCCA TGGGTGCTGA GCAATGGTCC GCCGATCTTT 
GGCAACGGCA GTGGACTGAT CCATCCGCCC GGGGTTGGAG CGGGTGCGCC GCCCCCGCCG 
AGACTTCCCA CACTTCCCGG AGTGGTGGGA ATCAGTGGGA ATCCCGGCCT GTTCAACGTA 
CCACCGCAAC ACACCGCCGT CACGCACTCC ACGCCCTCGA GCAGCACGAC AGCCGTGGGC 
GGTGGACGTT TGAAGGGTGG GGCCACCTCC ACGACGACCA CCAAGCATCC GGCTGGCCGC 
CATGATGGTC TGCCACCGCC GCCGCAACTG CACCACCACC ACAACCACCA CGGTGAAGAC 
ACTGCCTCAC CCACCAAGCC GAGCAGCAAG CTGCCGGCTG GCGGTAATGC CACTTCACCA 
TCCAACACGA GGATACTCAC GGGCGTCGGA GGTTCCGGAA CTGATGACGG AACCCTACTG 
ACCACAAAGA GCTCACCCAA CCGCCCACCG GGCACTGCGG CCAGTGGATC CGTTGTCCCC 
GGGAACGGCA GCGTGGTGCG CACCATCAAC AATATTAATT TGAACGCAGC CGGGATGTCC 
GGAGGCGATG ATGAGTCCAA GTTGTTTTGC GGCCCCACTC ATGCCCGCAA TTTGTACTGG 
AACATGACTC GAGTGGGTGA TGTGAATGTT CAGCCCTGTC CTGGCGGAGC AGCCGGCATC 
GCCAAGTGGC GTTGCGTTCT AATGAAGAGG ATACCCGACT CCGGCTACGA TGAGTACGAT 
GATGACATCA GTTCGACAAC TCCGGCACCC AGCGGTGGCG ACTGTCTGCA CAACAGCAGC 
AGCTGCGAGC CGCCGGTGAG CATGGCCCAC AAGGTAAACC AGCGTCTGCG CAACTTTGAG 
CCCACCTGGC ATCCCGCGAC ACCTGATCTG ACGCAATGCC GCAGCCTTTG GCTCAACAAT 
CTGGAAATGC GAGTAAACCA GCGGGACTCC TCCTTGATCT CCATCGCCAA CGATATGTCC 
GAAGTGACCA GTAGCAAAAC GCTCTACGGC GGCGACATGT TGGTCACCAC GAAGATTATC 
CAAACAGTGT CCGAGAAGAT GATGCACGAC AAGGAGACCT TCCCGGATCA GCGACAGCGC 
GAGGCTATGA TCATGGAGTT GTTGCATTGT GTGGTCAAAA CCGGCTCCAA CCTGCTGGAC 
GAATCGCAGC TGTCCTCGTG GTTGGATCTC AATCCGGAGG ACCAAATGCG TGTAGCCACA 
TCCTTGCTAA CTGGCCTGGA ATACAATGCC TTTCTGCTGG CGGATACGAT CATCAGGGAG 
CGCAGCGTGG TGCAAAAAGT CAAAAATATA TTGCTCTCCG TTCGAGTTCT GGAAACCAAG 
ACTATCCAGT CCAGCGTGGT CTTCCCAGAT TCGGATCAGT GGCCCTTGAG TTCGGATCGT 
ATTGAGCTGC CACGAGCTGC TCTAATAGAT AATAGTGAAG GCGGTCTGGT GCGAATTGTA 
TTCGCCGCCT TCGATCGCCT GGAATCCATT CTAAAGCCCA GCTATGATCA CTTCGATCTC 
AAGAGCTCCC GCAGTTACGC CATCCTGAGC AACGACAGCG ATGTCAACGC GGGGGAGATC 
CAACAGCGCC TACGCATCCT GAACAGCAAG GTGATCTCGG CCAGCTTGGG CAAGGGGCGT 
CACATACAAC TCTCCCAGCC CATAACCCTG ACACTGAAAC ATCTGAAGAC CGAGAATGTA 
ACGAATCCCA CCTGCGTGTT CTGGAACTAT ATTGACCATG CGTGGTCTGC CAACGGATGC 
AGTCTGGAGT CCACTAACCG CACGCACAGC GTCTGCAGTT GCAACCACCT GACAAACTTT 
GCCATACTAA TGGACGTTGT GGATGAGCAC CAGCATTCGT TGTTCACCAT GTTCGATGGA 
AACATGCGCA TATTCATCTA CATAAGCATC GGCATCTGCG TGGTCTTCAT AGTTATCGCC 
CTGCTAACGC TGAAGCTGTT CAATGGGGTC TTTGTGAAGG TAAGAAACGG CTCCAATCCC 
TTGCCGCATC AGCGGTCGGG CAGCAGACGC CAGCAAAACA ATATTCGCGA CCAGACCCAC 
GAGTCCTTGA CCCTGACCAC GCCAACCAGT CAGTCCAATG TGCCGCCGCC CAGTCATGGG 
AACACGAACT TTATCCAACA CAATTCCATC CGCAACTCAC ACCGCAACAA TCTGAACTAC 
AATGTCCAAC AGCAGCAGCA GCAACAGCAA CAACAAGTTG TTGCGGCAGC TGCTGCTGCT 
GTTGTGCTGT CCAATCAGCC GCAGCGAAAT ATGCAACATG CCATGAACAA CTTGAATCTG 
AATCTGCATC AGCACGGTCA GCAGACGGCT GCTGCTGCAG CTGCTGCTGC CTCAATAGCC 
GCTGCACTGC AGCAACATGC AGTTCAGGCC AGCAACGCCA GCAATAACCT CAACATCAGC 
CACAACTATC TGCAGCAGCA GCATGTCCAG CAGCAGCAGC AGCAGCAGCG TGGCCAGCAG 
CCGCAGCCAC ATCCGCACCG CAATCACAAC CTTAATGTGG ACGGCAATGG ATTGGATAAC 
ACGAACAACA TCATTATGCA GGCCAACATG GACGACTTAC AATATAAGTG GTGGTGGTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260808.1
CDS	804..3806
Translation	MLPTILSISYEHISLDLSKYQTAYACEGKKLTIECDPGDVINLIRANYGRFSITICNDHGNVEWSVNCMFPKSLSVLNSRCAHKQSCGVLAATSMFGDPCPGTHKYLEAHYQCISAAQTSTTTNRPSPPPWVLSNGPPIFGNGSGLIHPPGVGAGAPPPPRLPTLPGVVGISGNPGLFNVPPQHTAVTHSTPSSSTTAVGGGRLKGGATSTTTTKHPAGRHDGLPPPPQLHHHHNHHGEDTASPTKPSSKLPAGGNATSPSNTRILTGVGGSGTDDGTLLTTKSSPNRPPGTAASGSVVPGNGSVVRTINNINLNAAGMSGGDDESKLFCGPTHARNLYWNMTRVGDVNVQPCPGGAAGIAKWRCVLMKRIPDSGYDEYDDDISSTTPAPSGGDCLHNSSSCEPPVSMAHKVNQRLRNFEPTWHPATPDLTQCRSLWLNNLEMRVNQRDSSLISIANDMSEVTSSKTLYGGDMLVTTKIIQTVSEKMMHDKETFPDQRQREAMIMELLHCVVKTGSNLLDESQLSSWLDLNPEDQMRVATSLLTGLEYNAFLLADTIIRERSVVQKVKNILLSVRVLETKTIQSSVVFPDSDQWPLSSDRIELPRAALIDNSEGGLVRIVFAAFDRLESILKPSYDHFDLKSSRSYAILSNDSDVNAGEIQQRLRILNSKVISASLGKGRHIQLSQPITLTLKHLKTENVTNPTCVFWNYIDHAWSANGCSLESTNRTHSVCSCNHLTNFAILMDVVDEHQHSLFTMFDGNMRIFIYISIGICVVFIVIALLTLKLFNGVFVKVRNGSNPLPHQRSGSRRQQNNIRDQTHESLTLTTPTSQSNVPPPSHGNTNFIQHNSIRNSHRNNLNYNVQQQQQQQQQQVVAAAAAAVVLSNQPQRNMQHAMNNLNLNLHQHGQQTAAAAAAAASIAAALQQHAVQASNASNNLNISHNYLQQQHVQQQQQQQRGQQPQPHPHRNHNLNVDGNGLDNTNNIIMQANMDDLQYKWWWW

Target ORF information:

RefSeq Version	NM_001273879.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Calcium-independent receptor for alpha-latrotoxin (Cirl), transcript variant J, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273879.2

ORF Insert Sequence:

ATGCTACCAA CCATATTGTC AATATCCTAT GAGCATATAT CGTTAGATCT GTCCAAGTAC 
CAAACCGCCT ACGCCTGCGA AGGTAAGAAA CTGACCATCG AGTGCGATCC CGGCGATGTG 
ATCAACCTCA TTCGGGCCAA CTATGGCCGC TTCTCGATTA CCATCTGCAA TGACCACGGG 
AATGTGGAGT GGAGTGTTAA CTGCATGTTT CCCAAGTCAC TCAGCGTACT GAACTCAAGA 
TGTGCCCACA AGCAGAGCTG CGGCGTGTTG GCAGCCACGA GCATGTTCGG GGATCCCTGT 
CCCGGTACCC ACAAGTATCT GGAGGCACAC TACCAGTGCA TAAGTGCAGC CCAAACTTCG 
ACGACGACCA ACAGGCCCAG TCCGCCGCCA TGGGTGCTGA GCAATGGTCC GCCGATCTTT 
GGCAACGGCA GTGGACTGAT CCATCCGCCC GGGGTTGGAG CGGGTGCGCC GCCCCCGCCG 
AGACTTCCCA CACTTCCCGG AGTGGTGGGA ATCAGTGGGA ATCCCGGCCT GTTCAACGTA 
CCACCGCAAC ACACCGCCGT CACGCACTCC ACGCCCTCGA GCAGCACGAC AGCCGTGGGC 
GGTGGACGTT TGAAGGGTGG GGCCACCTCC ACGACGACCA CCAAGCATCC GGCTGGCCGC 
CATGATGGTC TGCCACCGCC GCCGCAACTG CACCACCACC ACAACCACCA CGGTGAAGAC 
ACTGCCTCAC CCACCAAGCC GAGCAGCAAG CTGCCGGCTG GCGGTAATGC CACTTCACCA 
TCCAACACGA GGATACTCAC GGGCGTCGGA GGTTCCGGAA CTGATGACGG AACCCTACTG 
ACCACAAAGA GCTCACCCAA CCGCCCACCG GGCACTGCGG CCAGTGGATC CGTTGTCCCC 
GGGAACGGCA GCGTGGTGCG CACCATCAAC AATATTAATT TGAACGCAGC CGGGATGTCC 
GGAGGCGATG ATGAGTCCAA GTTGTTTTGC GGCCCCACTC ATGCCCGCAA TTTGTACTGG 
AACATGACTC GAGTGGGTGA TGTGAATGTT CAGCCCTGTC CTGGCGGAGC AGCCGGCATC 
GCCAAGTGGC GTTGCGTTCT AATGAAGAGG ATACCCGACT CCGGCTACGA TGAGTACGAT 
GATGACATCA GTTCGACAAC TCCGGCACCC AGCGGTGGCG ACTGTCTGCA CAACAGCAGC 
AGCTGCGAGC CGCCGGTGAG CATGGCCCAC AAGGTAAACC AGCGTCTGCG CAACTTTGAG 
CCCACCTGGC ATCCCGCGAC ACCTGATCTG ACGCAATGCC GCAGCCTTTG GCTCAACAAT 
CTGGAAATGC GAGTAAACCA GCGGGACTCC TCCTTGATCT CCATCGCCAA CGATATGTCC 
GAAGTGACCA GTAGCAAAAC GCTCTACGGC GGCGACATGT TGGTCACCAC GAAGATTATC 
CAAACAGTGT CCGAGAAGAT GATGCACGAC AAGGAGACCT TCCCGGATCA GCGACAGCGC 
GAGGCTATGA TCATGGAGTT GTTGCATTGT GTGGTCAAAA CCGGCTCCAA CCTGCTGGAC 
GAATCGCAGC TGTCCTCGTG GTTGGATCTC AATCCGGAGG ACCAAATGCG TGTAGCCACA 
TCCTTGCTAA CTGGCCTGGA ATACAATGCC TTTCTGCTGG CGGATACGAT CATCAGGGAG 
CGCAGCGTGG TGCAAAAAGT CAAAAATATA TTGCTCTCCG TTCGAGTTCT GGAAACCAAG 
ACTATCCAGT CCAGCGTGGT CTTCCCAGAT TCGGATCAGT GGCCCTTGAG TTCGGATCGT 
ATTGAGCTGC CACGAGCTGC TCTAATAGAT AATAGTGAAG GCGGTCTGGT GCGAATTGTA 
TTCGCCGCCT TCGATCGCCT GGAATCCATT CTAAAGCCCA GCTATGATCA CTTCGATCTC 
AAGAGCTCCC GCAGTTACGC CATCCTGAGC AACGACAGCG ATGTCAACGC GGGGGAGATC 
CAACAGCGCC TACGCATCCT GAACAGCAAG GTGATCTCGG CCAGCTTGGG CAAGGGGCGT 
CACATACAAC TCTCCCAGCC CATAACCCTG ACACTGAAAC ATCTGAAGAC CGAGAATGTA 
ACGAATCCCA CCTGCGTGTT CTGGAACTAT ATTGACCATG CGTGGTCTGC CAACGGATGC 
AGTCTGGAGT CCACTAACCG CACGCACAGC GTCTGCAGTT GCAACCACCT GACAAACTTT 
GCCATACTAA TGGACGTTGT GGATGAGCAC CAGCATTCGT TGTTCACCAT GTTCGATGGA 
AACATGCGCA TATTCATCTA CATAAGCATC GGCATCTGCG TGGTCTTCAT AGTTATCGCC 
CTGCTAACGC TGAAGCTGTT CAATGGGGTC TTTGTGAAGG TAAGAAACGG CTCCAATCCC 
TTGCCGCATC AGCGGTCGGG CAGCAGACGC CAGCAAAACA ATATTCGCGA CCAGACCCAC 
GAGTCCTTGA CCCTGACCAC GCCAACCAGT CAGTCCAATG TGCCGCCGCC CAGTCATGGG 
AACACGAACT TTATCCAACA CAATTCCATC CGCAACTCAC ACCGCAACAA TCTGAACTAC 
AATGTCCAAC AGCAGCAGCA GCAACAGCAA CAACAAGTTG TTGCGGCAGC TGCTGCTGCT 
GTTGTGCTGT CCAATCAGCC GCAGCGAAAT ATGCAACATG CCATGAACAA CTTGAATCTG 
AATCTGCATC AGCACGGTCA GCAGACGGCT GCTGCTGCAG CTGCTGCTGC CTCAATAGCC 
GCTGCACTGC AGCAACATGC AGTTCAGGCC AGCAACGCCA GCAATAACCT CAACATCAGC 
CACAACTATC TGCAGCAGCA GCATGTCCAG CAGCAGCAGC AGCAGCAGCG TGGCCAGCAG 
CCGCAGCCAC ATCCGCACCG CAATCACAAC CTTAATGTGG ACGGCAATGG ATTGGATAAC 
ACGAACAACA TCATTATGCA GGCCAACATG GACGACTTAC AATATAAGTG GTGGTGGTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cirl ( NM_001169619.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163090.1 Drosophila melanogaster Calcium-independent receptor for alpha-latrotoxin (Cirl), transcript variant E, mRNA.

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CloneID	OFa09300
Clone ID Related Accession (Same CDS sequence)	NM_001169619.4
Accession Version	NM_001169619.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4920bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Calcium-independent receptor for alpha-latrotoxin, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001169619.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTCCCA AGTCACTCAG CGTACTGAAC TCAAGATGTG CCCACAAGCA GAGCTGCGGC 
GTGTTGGCAG CCACGAGCAT GTTCGGGGAT CCCTGTCCCG GTACCCACAA GTATCTGGAG 
GCACACTACC AGTGCATAAG TGCAGCCCAA ACTTCGACGA CGACCAACAG GCCCAGTCCG 
CCGCCATGGG TGCTGAGCAA TGGTCCGCCG ATCTTTGGCA ACGGCAGTGG ACTGATCCAT 
CCGCCCGGGG TTGGAGCGGG TGCGCCGCCC CCGCCGAGAC TTCCCACACT TCCCGGAGTG 
GTGGGAATCA GTGGGAATCC CGGCCTGTTC AACGTACCAC CGCAACACAC CGCCGTCACG 
CACTCCACGC CCTCGAGCAG CACGACAGCC GTGGGCGGTG GACGTTTGAA GGGTGGGGCC 
ACCTCCACGA CGACCACCAA GCATCCGGCT GGCCGCCATG ATGGTCTGCC ACCGCCGCCG 
CAACTGCACC ACCACCACAA CCACCACGGT GAAGACACTG CCTCACCCAC CAAGCCGAGC 
AGCAAGCTGC CGGCTGGCGG TAATGCCACT TCACCATCCA ACACGAGGAT ACTCACGGGC 
GTCGGAGGTT CCGGAACTGA TGACGGAACC CTACTGACCA CAAAGAGCTC ACCCAACCGC 
CCACCGGGCA CTGCGGCCAG TGGATCCGTT GTCCCCGGGA ACGGCAGCGT GGTGCGCACC 
ATCAACAATA TTAATTTGAA CGCAGCCGGG ATGTCCGGAG GCGATGATGA GTCCAAGTTG 
TTTTGCGGCC CCACTCATGC CCGCAATTTG TACTGGAACA TGACTCGAGT GGGTGATGTG 
AATGTTCAGC CCTGTCCTGG CGGAGCAGCC GGCATCGCCA AGTGGCGTTG CGTTCTAATG 
AAGAGGATAC CCGACTCCGG CTACGATGAG TACGATGATG ACATCAGTTC GACAACTCCG 
GCACCCAGCG GTGGCGACTG TCTGCACAAC AGCAGCAGCT GCGAGCCGCC GGTGAGCATG 
GCCCACAAGG TAAACCAGCG TCTGCGCAAC TTTGAGCCCA CCTGGCATCC CGCGACACCT 
GATCTGACGC AATGCCGCAG CCTTTGGCTC AACAATCTGG AAATGCGAGT AAACCAGCGG 
GACTCCTCCT TGATCTCCAT CGCCAACGAT ATGTCCGAAG TGACCAGTAG CAAAACGCTC 
TACGGCGGCG ACATGTTGGT CACCACGAAG ATTATCCAAA CAGTGTCCGA GAAGATGATG 
CACGACAAGG AGACCTTCCC GGATCAGCGA CAGCGCGAGG CTATGATCAT GGAGTTGTTG 
CATTGTGTGG TCAAAACCGG CTCCAACCTG CTGGACGAAT CGCAGCTGTC CTCGTGGTTG 
GATCTCAATC CGGAGGACCA AATGCGTGTA GCCACATCCT TGCTAACTGG CCTGGAATAC 
AATGCCTTTC TGCTGGCGGA TACGATCATC AGGGAGCGCA GCGTGGTGCA AAAAGTCAAA 
AATATATTGC TCTCCGTTCG AGTTCTGGAA ACCAAGACTA TCCAGTCCAG CGTGGTCTTC 
CCAGATTCGG ATCAGTGGCC CTTGAGTTCG GATCGTATTG AGCTGCCACG AGCTGCTCTA 
ATAGATAATA GTGAAGGCGG TCTGGTGCGA ATTGTATTCG CCGCCTTCGA TCGCCTGGAA 
TCCATTCTAA AGCCCAGCTA TGATCACTTC GATCTCAAGA GCTCCCGCAG TTACGCCATC 
CTGAGCAACG ACAGCGATGT CAACGCGGGG GAGATCCAAC AGCGCCTACG CATCCTGAAC 
AGCAAGGTGA TCTCGGCCAG CTTGGGCAAG GGGCGTCACA TACAACTCTC CCAGCCCATA 
ACCCTGACAC TGAAACATCT GAAGACCGAG AATGTAACGA ATCCCACCTG CGTGTTCTGG 
AACTATATTG ACCATGCGTG GTCTGCCAAC GGATGCAGTC TGGAGTCCAC TAACCGCACG 
CACAGCGTCT GCAGTTGCAA CCACCTGACA AACTTTGCCA TACTAATGGA CGTTGTGGAT 
GAGCACCAGC ATTCGTTGTT CACCATGTTC GATGGAAACA TGCGCATATT CATCTACATA 
AGCATCGGCA TCTGCGTGGT CTTCATAGTT ATCGCCCTGC TAACGCTGAA GCTGTTCAAT 
GGGGTCTTTG TGAAGTCCGC GCGCACCTCG ATCTATACCA GCATTTACCT TTGCCTCCTG 
GCCATCGAGC TGCTCTTTCT CCTGGGCATT GAACAGACCG AAACAAGCAT TTTCTGCGGC 
TTCATTACTA TTTTCCTACA CTGTGCCATC CTATCGGGCA CCGCCTGGTT CTGTTACGAA 
GCCTTCCATT CGTACTCAAC GCTCACCTCG GACGAGCTCC TGCTGGAGGT GGACCAGACG 
CCCAAGGTGA ACTGCTACTA CCTCTTGTCC TACGGACTGT CGCTGAGCGT GGTGGCCATC 
TCGCTGGTCA TCGATCCCAG CACCTATACC CAAAACGATT ATTGCGTGCT GATGGAGGCG 
AATGCCTTGT TTTATGCCAC CTTTGTAATA CCAGTGCTTG TCTTCTTTGT GGCTGCCATT 
GGTTACACAT TCCTCTCCTG GATTATAATG TGCCGCAAAA GTCGCACGGG TCTAAAGACC 
AAGGAACATA CTCGCCTCGC TAGCGTGCGG TTCGACATAC GCTGCTCCTT TGTGTTCCTC 
TTGCTGCTCA GCGCTGTTTG GTGCTCGGCC TACTTCTATT TGCGAGGAGC CAAAATGGAC 
GATGACACGG CTGATGTGTA TGGATACTGC TTCATCTGCT TCAACACATT GCTGGGGCTC 
TATATCTTCG TGTTCCATTG CATTCAAAAC GAAAAGATCC GGCGGGAGTA TCGGAAGTAT 
GTGAGACAGC ACGCTTGGCT GCCCAAGTGC TTGCGCTGCT CGAAAACATC AATTTCCTCG 
GGCATTGTTA CCGGCAATGG ACCCACAGCC GGAACCCTTT GCAGCGTCTC CACGTCCAAG 
AAGCCCAAGC TGCCGTTAGG AGTGAGCGAA GAGGCGCATG ACGATCCCCA GCAGCAACAG 
CAGACACCAG TGCCCATCAC AGAGGATGCC ATTATGGGAG CCACCTCTGA TTGTGAACTG 
AACGAGGCCC AGCAAAGAAG AACCCTAAAA AGTGGCCTAA TGACGGGCAC ACTACAGGCT 
CCACCGCAGA CCCTTGGTGG CCATGTTGTG CTCGAAAGAG GTAGCACTCT CCGCTCCACT 
GGTCATGCCT CACCCACCAG CTCTGCCGGG TCCACACACC TGATTTTTGC GCACAAGCAA 
CAACAACAAC AGCAGCAACA GGGACCTTTG GGCGAGTCTT ACTACCATCA GCCGGACTAC 
TACAGCTGGA AGCAACCATC AACTGGAACA GGAGGATTGA AAACACCGCG GGAGTACTAC 
AATAATGCGG GTGCTGCTGC ATCATCGCCG CAACAGGCGC ACGAGGTATT CTACTGGACT 
CAAAAGCCGA ACAGCGGCCA CAATGGCAAA AAGAAGAGGG GCGCCGGAGG AGTTCCCGCC 
TCGCCTAGTG GATCTCTGCA CAGTCGCACG GCCGCCGCCT CCCAGGTGCT TTTCTATCCA 
TCGTACAAGA AGACCAAGCC TGGCCAGCCA ACAGGCTATC CGCAATACGC GGAGGCGTTG 
GACCCACCAC TAGCCACTGG CAATGCGGCT GCCTACTACC AGCAGCAGCA ACAGTTGCGT 
CGCCAGCAGC TACATCAGCA GCAGCAACAG CAGCAGCAGC AGCAACTCTC CTCGGACGAG 
GAGCAGGCCG AGCAACATGC TCACCTGTTG CACCTGCAAC GACGAGCTGG TAGCCAGCAG 
CAGCTCCCTG CTCCACCGCC ACACATGGCG CAGTACCAGC AGGAGTTTAT GCAGCGCCAG 
TATAGAAATA AGCATTCCAA CTGTGATCTG GGCATGGGCG ATGCCTACTA CAACCAAGGC 
AGCGTCGGCG GCGCGGATGG TGGGCCGGTC TACGAGGAGA TCCTCAGCAA CCGCAACTCG 
GATGTGCAGC ATTACGAGGT GGGTGACTTC GATGTGGACG AGGTGTACAA CAATAGCGTT 
GGCACTGGCG TCTTCAACAA CATGAGAGCG GCGGTGGCCG CCGGCGGTAG TCGGTACGGC 
GGCGGAAGCC TGAGTGGCGG CAGTGTCTCG TCCAGGAGCC AACAGCAGCA GCTCAAGAAG 
CAGCAGCAAC AACAGTCGCT GGCTCAGCAA AGATCGGCTC GACGCTGCAC GGCGGATGAC 
GATGACGACG AAGACGAGGA GGAGGATGAG GAGGCAACGG CCGCGGAGCA ATTGCACGAC 
AGCGTCTGTG ATGAGGATGA GGAGGAGGAC GAGAGCGACT TGGAGGATGA TGCTCATGGA 
TTGCCACCGC AGAGCGATGA GCGGATGCGT CGTCTGATGG CGATGCAGGA CGAGGATTTT 
AAGCGGCGGT TTCAACGTCA GCTGCGCAAA CATGGAGCGC CTCTTGACTA CGGGGCTTTG 
CCACCAGGAG CAGGACCGCA ACCCGAGCAC AACGGTGCGG TTTTTGGGGT TAGCGGCGGC 
GTTGGTGAGG GCTCCCTGCG TGGCGCATTC CGGCAGCAGC AGCAGCAAGC ACTGAATGCC 
AAGTCGCCAG GCGGCCGTTT GGCGGTAAAT GAGCTATTCG GTCACGGCAA CTCGGGACCA 
CCGCTGCCGC CGGCGAATCA GACGCCCGCG CAGAAGCGCC AGCAGCTACA GAAACTGTCA 
CCACAGTCCA CCACATCATC GTCGTCGCAT ACATCACACA GCAATCCCAA TCCGCATCCC 
CACCAGCTAA CCCATCCGCA TCCCCATCAG CATCCTCCGC ACCATCAGCA GCGCCACCTG 
TCGGCCATGC TCGATGAGAA CAACACGGTG CGATGTTATC TGGAACCACT GGCTAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163090.1
CDS	313..5232
Translation	MFPKSLSVLNSRCAHKQSCGVLAATSMFGDPCPGTHKYLEAHYQCISAAQTSTTTNRPSPPPWVLSNGPPIFGNGSGLIHPPGVGAGAPPPPRLPTLPGVVGISGNPGLFNVPPQHTAVTHSTPSSSTTAVGGGRLKGGATSTTTTKHPAGRHDGLPPPPQLHHHHNHHGEDTASPTKPSSKLPAGGNATSPSNTRILTGVGGSGTDDGTLLTTKSSPNRPPGTAASGSVVPGNGSVVRTINNINLNAAGMSGGDDESKLFCGPTHARNLYWNMTRVGDVNVQPCPGGAAGIAKWRCVLMKRIPDSGYDEYDDDISSTTPAPSGGDCLHNSSSCEPPVSMAHKVNQRLRNFEPTWHPATPDLTQCRSLWLNNLEMRVNQRDSSLISIANDMSEVTSSKTLYGGDMLVTTKIIQTVSEKMMHDKETFPDQRQREAMIMELLHCVVKTGSNLLDESQLSSWLDLNPEDQMRVATSLLTGLEYNAFLLADTIIRERSVVQKVKNILLSVRVLETKTIQSSVVFPDSDQWPLSSDRIELPRAALIDNSEGGLVRIVFAAFDRLESILKPSYDHFDLKSSRSYAILSNDSDVNAGEIQQRLRILNSKVISASLGKGRHIQLSQPITLTLKHLKTENVTNPTCVFWNYIDHAWSANGCSLESTNRTHSVCSCNHLTNFAILMDVVDEHQHSLFTMFDGNMRIFIYISIGICVVFIVIALLTLKLFNGVFVKSARTSIYTSIYLCLLAIELLFLLGIEQTETSIFCGFITIFLHCAILSGTAWFCYEAFHSYSTLTSDELLLEVDQTPKVNCYYLLSYGLSLSVVAISLVIDPSTYTQNDYCVLMEANALFYATFVIPVLVFFVAAIGYTFLSWIIMCRKSRTGLKTKEHTRLASVRFDIRCSFVFLLLLSAVWCSAYFYLRGAKMDDDTADVYGYCFICFNTLLGLYIFVFHCIQNEKIRREYRKYVRQHAWLPKCLRCSKTSISSGIVTGNGPTAGTLCSVSTSKKPKLPLGVSEEAHDDPQQQQQTPVPITEDAIMGATSDCELNEAQQRRTLKSGLMTGTLQAPPQTLGGHVVLERGSTLRSTGHASPTSSAGSTHLIFAHKQQQQQQQQGPLGESYYHQPDYYSWKQPSTGTGGLKTPREYYNNAGAAASSPQQAHEVFYWTQKPNSGHNGKKKRGAGGVPASPSGSLHSRTAAASQVLFYPSYKKTKPGQPTGYPQYAEALDPPLATGNAAAYYQQQQQLRRQQLHQQQQQQQQQQLSSDEEQAEQHAHLLHLQRRAGSQQQLPAPPPHMAQYQQEFMQRQYRNKHSNCDLGMGDAYYNQGSVGGADGGPVYEEILSNRNSDVQHYEVGDFDVDEVYNNSVGTGVFNNMRAAVAAGGSRYGGGSLSGGSVSSRSQQQQLKKQQQQQSLAQQRSARRCTADDDDDEDEEEDEEATAAEQLHDSVCDEDEEEDESDLEDDAHGLPPQSDERMRRLMAMQDEDFKRRFQRQLRKHGAPLDYGALPPGAGPQPEHNGAVFGVSGGVGEGSLRGAFRQQQQQALNAKSPGGRLAVNELFGHGNSGPPLPPANQTPAQKRQQLQKLSPQSTTSSSSHTSHSNPNPHPHQLTHPHPHQHPPHHQQRHLSAMLDENNTVRCYLEPLAK

Target ORF information:

RefSeq Version	NM_001169619.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Calcium-independent receptor for alpha-latrotoxin (Cirl), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169619.4

ORF Insert Sequence:

ATGTTTCCCA AGTCACTCAG CGTACTGAAC TCAAGATGTG CCCACAAGCA GAGCTGCGGC 
GTGTTGGCAG CCACGAGCAT GTTCGGGGAT CCCTGTCCCG GTACCCACAA GTATCTGGAG 
GCACACTACC AGTGCATAAG TGCAGCCCAA ACTTCGACGA CGACCAACAG GCCCAGTCCG 
CCGCCATGGG TGCTGAGCAA TGGTCCGCCG ATCTTTGGCA ACGGCAGTGG ACTGATCCAT 
CCGCCCGGGG TTGGAGCGGG TGCGCCGCCC CCGCCGAGAC TTCCCACACT TCCCGGAGTG 
GTGGGAATCA GTGGGAATCC CGGCCTGTTC AACGTACCAC CGCAACACAC CGCCGTCACG 
CACTCCACGC CCTCGAGCAG CACGACAGCC GTGGGCGGTG GACGTTTGAA GGGTGGGGCC 
ACCTCCACGA CGACCACCAA GCATCCGGCT GGCCGCCATG ATGGTCTGCC ACCGCCGCCG 
CAACTGCACC ACCACCACAA CCACCACGGT GAAGACACTG CCTCACCCAC CAAGCCGAGC 
AGCAAGCTGC CGGCTGGCGG TAATGCCACT TCACCATCCA ACACGAGGAT ACTCACGGGC 
GTCGGAGGTT CCGGAACTGA TGACGGAACC CTACTGACCA CAAAGAGCTC ACCCAACCGC 
CCACCGGGCA CTGCGGCCAG TGGATCCGTT GTCCCCGGGA ACGGCAGCGT GGTGCGCACC 
ATCAACAATA TTAATTTGAA CGCAGCCGGG ATGTCCGGAG GCGATGATGA GTCCAAGTTG 
TTTTGCGGCC CCACTCATGC CCGCAATTTG TACTGGAACA TGACTCGAGT GGGTGATGTG 
AATGTTCAGC CCTGTCCTGG CGGAGCAGCC GGCATCGCCA AGTGGCGTTG CGTTCTAATG 
AAGAGGATAC CCGACTCCGG CTACGATGAG TACGATGATG ACATCAGTTC GACAACTCCG 
GCACCCAGCG GTGGCGACTG TCTGCACAAC AGCAGCAGCT GCGAGCCGCC GGTGAGCATG 
GCCCACAAGG TAAACCAGCG TCTGCGCAAC TTTGAGCCCA CCTGGCATCC CGCGACACCT 
GATCTGACGC AATGCCGCAG CCTTTGGCTC AACAATCTGG AAATGCGAGT AAACCAGCGG 
GACTCCTCCT TGATCTCCAT CGCCAACGAT ATGTCCGAAG TGACCAGTAG CAAAACGCTC 
TACGGCGGCG ACATGTTGGT CACCACGAAG ATTATCCAAA CAGTGTCCGA GAAGATGATG 
CACGACAAGG AGACCTTCCC GGATCAGCGA CAGCGCGAGG CTATGATCAT GGAGTTGTTG 
CATTGTGTGG TCAAAACCGG CTCCAACCTG CTGGACGAAT CGCAGCTGTC CTCGTGGTTG 
GATCTCAATC CGGAGGACCA AATGCGTGTA GCCACATCCT TGCTAACTGG CCTGGAATAC 
AATGCCTTTC TGCTGGCGGA TACGATCATC AGGGAGCGCA GCGTGGTGCA AAAAGTCAAA 
AATATATTGC TCTCCGTTCG AGTTCTGGAA ACCAAGACTA TCCAGTCCAG CGTGGTCTTC 
CCAGATTCGG ATCAGTGGCC CTTGAGTTCG GATCGTATTG AGCTGCCACG AGCTGCTCTA 
ATAGATAATA GTGAAGGCGG TCTGGTGCGA ATTGTATTCG CCGCCTTCGA TCGCCTGGAA 
TCCATTCTAA AGCCCAGCTA TGATCACTTC GATCTCAAGA GCTCCCGCAG TTACGCCATC 
CTGAGCAACG ACAGCGATGT CAACGCGGGG GAGATCCAAC AGCGCCTACG CATCCTGAAC 
AGCAAGGTGA TCTCGGCCAG CTTGGGCAAG GGGCGTCACA TACAACTCTC CCAGCCCATA 
ACCCTGACAC TGAAACATCT GAAGACCGAG AATGTAACGA ATCCCACCTG CGTGTTCTGG 
AACTATATTG ACCATGCGTG GTCTGCCAAC GGATGCAGTC TGGAGTCCAC TAACCGCACG 
CACAGCGTCT GCAGTTGCAA CCACCTGACA AACTTTGCCA TACTAATGGA CGTTGTGGAT 
GAGCACCAGC ATTCGTTGTT CACCATGTTC GATGGAAACA TGCGCATATT CATCTACATA 
AGCATCGGCA TCTGCGTGGT CTTCATAGTT ATCGCCCTGC TAACGCTGAA GCTGTTCAAT 
GGGGTCTTTG TGAAGTCCGC GCGCACCTCG ATCTATACCA GCATTTACCT TTGCCTCCTG 
GCCATCGAGC TGCTCTTTCT CCTGGGCATT GAACAGACCG AAACAAGCAT TTTCTGCGGC 
TTCATTACTA TTTTCCTACA CTGTGCCATC CTATCGGGCA CCGCCTGGTT CTGTTACGAA 
GCCTTCCATT CGTACTCAAC GCTCACCTCG GACGAGCTCC TGCTGGAGGT GGACCAGACG 
CCCAAGGTGA ACTGCTACTA CCTCTTGTCC TACGGACTGT CGCTGAGCGT GGTGGCCATC 
TCGCTGGTCA TCGATCCCAG CACCTATACC CAAAACGATT ATTGCGTGCT GATGGAGGCG 
AATGCCTTGT TTTATGCCAC CTTTGTAATA CCAGTGCTTG TCTTCTTTGT GGCTGCCATT 
GGTTACACAT TCCTCTCCTG GATTATAATG TGCCGCAAAA GTCGCACGGG TCTAAAGACC 
AAGGAACATA CTCGCCTCGC TAGCGTGCGG TTCGACATAC GCTGCTCCTT TGTGTTCCTC 
TTGCTGCTCA GCGCTGTTTG GTGCTCGGCC TACTTCTATT TGCGAGGAGC CAAAATGGAC 
GATGACACGG CTGATGTGTA TGGATACTGC TTCATCTGCT TCAACACATT GCTGGGGCTC 
TATATCTTCG TGTTCCATTG CATTCAAAAC GAAAAGATCC GGCGGGAGTA TCGGAAGTAT 
GTGAGACAGC ACGCTTGGCT GCCCAAGTGC TTGCGCTGCT CGAAAACATC AATTTCCTCG 
GGCATTGTTA CCGGCAATGG ACCCACAGCC GGAACCCTTT GCAGCGTCTC CACGTCCAAG 
AAGCCCAAGC TGCCGTTAGG AGTGAGCGAA GAGGCGCATG ACGATCCCCA GCAGCAACAG 
CAGACACCAG TGCCCATCAC AGAGGATGCC ATTATGGGAG CCACCTCTGA TTGTGAACTG 
AACGAGGCCC AGCAAAGAAG AACCCTAAAA AGTGGCCTAA TGACGGGCAC ACTACAGGCT 
CCACCGCAGA CCCTTGGTGG CCATGTTGTG CTCGAAAGAG GTAGCACTCT CCGCTCCACT 
GGTCATGCCT CACCCACCAG CTCTGCCGGG TCCACACACC TGATTTTTGC GCACAAGCAA 
CAACAACAAC AGCAGCAACA GGGACCTTTG GGCGAGTCTT ACTACCATCA GCCGGACTAC 
TACAGCTGGA AGCAACCATC AACTGGAACA GGAGGATTGA AAACACCGCG GGAGTACTAC 
AATAATGCGG GTGCTGCTGC ATCATCGCCG CAACAGGCGC ACGAGGTATT CTACTGGACT 
CAAAAGCCGA ACAGCGGCCA CAATGGCAAA AAGAAGAGGG GCGCCGGAGG AGTTCCCGCC 
TCGCCTAGTG GATCTCTGCA CAGTCGCACG GCCGCCGCCT CCCAGGTGCT TTTCTATCCA 
TCGTACAAGA AGACCAAGCC TGGCCAGCCA ACAGGCTATC CGCAATACGC GGAGGCGTTG 
GACCCACCAC TAGCCACTGG CAATGCGGCT GCCTACTACC AGCAGCAGCA ACAGTTGCGT 
CGCCAGCAGC TACATCAGCA GCAGCAACAG CAGCAGCAGC AGCAACTCTC CTCGGACGAG 
GAGCAGGCCG AGCAACATGC TCACCTGTTG CACCTGCAAC GACGAGCTGG TAGCCAGCAG 
CAGCTCCCTG CTCCACCGCC ACACATGGCG CAGTACCAGC AGGAGTTTAT GCAGCGCCAG 
TATAGAAATA AGCATTCCAA CTGTGATCTG GGCATGGGCG ATGCCTACTA CAACCAAGGC 
AGCGTCGGCG GCGCGGATGG TGGGCCGGTC TACGAGGAGA TCCTCAGCAA CCGCAACTCG 
GATGTGCAGC ATTACGAGGT GGGTGACTTC GATGTGGACG AGGTGTACAA CAATAGCGTT 
GGCACTGGCG TCTTCAACAA CATGAGAGCG GCGGTGGCCG CCGGCGGTAG TCGGTACGGC 
GGCGGAAGCC TGAGTGGCGG CAGTGTCTCG TCCAGGAGCC AACAGCAGCA GCTCAAGAAG 
CAGCAGCAAC AACAGTCGCT GGCTCAGCAA AGATCGGCTC GACGCTGCAC GGCGGATGAC 
GATGACGACG AAGACGAGGA GGAGGATGAG GAGGCAACGG CCGCGGAGCA ATTGCACGAC 
AGCGTCTGTG ATGAGGATGA GGAGGAGGAC GAGAGCGACT TGGAGGATGA TGCTCATGGA 
TTGCCACCGC AGAGCGATGA GCGGATGCGT CGTCTGATGG CGATGCAGGA CGAGGATTTT 
AAGCGGCGGT TTCAACGTCA GCTGCGCAAA CATGGAGCGC CTCTTGACTA CGGGGCTTTG 
CCACCAGGAG CAGGACCGCA ACCCGAGCAC AACGGTGCGG TTTTTGGGGT TAGCGGCGGC 
GTTGGTGAGG GCTCCCTGCG TGGCGCATTC CGGCAGCAGC AGCAGCAAGC ACTGAATGCC 
AAGTCGCCAG GCGGCCGTTT GGCGGTAAAT GAGCTATTCG GTCACGGCAA CTCGGGACCA 
CCGCTGCCGC CGGCGAATCA GACGCCCGCG CAGAAGCGCC AGCAGCTACA GAAACTGTCA 
CCACAGTCCA CCACATCATC GTCGTCGCAT ACATCACACA GCAATCCCAA TCCGCATCCC 
CACCAGCTAA CCCATCCGCA TCCCCATCAG CATCCTCCGC ACCATCAGCA GCGCCACCTG 
TCGGCCATGC TCGATGAGAA CAACACGGTG CGATGTTATC TGGAACCACT GGCTAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cirl ( NM_136553.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610397.4 Drosophila melanogaster Calcium-independent receptor for alpha-latrotoxin (Cirl), transcript variant G, mRNA.

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CloneID	OFa12785
Clone ID Related Accession (Same CDS sequence)	NM_136553.5
Accession Version	NM_136553.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Calcium-independent receptor for alpha-latrotoxin, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_136553.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAAGT ACCAAACCGC CTACGCCTGC GAAGGTAAGA AACTGACCAT CGAGTGCGAT 
CCCGGCGATG TGATCAACCT CATTCGGGCC AACTATGGCC GCTTCTCGAT TACCATCTGC 
AATGACCACG GGAATGTGGA GTGGAGTGTT AACTGCATGT TTCCCAAGTC ACTCAGCGTA 
CTGAACTCAA GATGTGCCCA CAAGCAGAGC TGCGGCGTGT TGGCAGCCAC GAGCATGTTC 
GGGGATCCCT GTCCCGGTAC CCACAAGTAT CTGGAGGCAC ACTACCAGTG CATAAGTGCA 
GCCCAAACTT CGACGACGAC CAACAGGCCC AGTCCGCCGC CATGGGTGCT GAGCAATGGT 
CCGCCGATCT TTGGCAACGG CAGTGGACTG ATCCATCCGC CCGGGGTTGG AGCGGGTGCG 
CCGCCCCCGC CGAGACTTCC CACACTTCCC GGAGTGGTGG GAATCAGTGG GAATCCCGGC 
CTGTTCAACG TACCACCGCA ACACACCGCC GTCACGCACT CCACGCCCTC GAGCAGCACG 
ACAGCCGTGG GCGGTGGACG TTTGAAGGGT GGGGCCACCT CCACGACGAC CACCAAGCAT 
CCGGCTGGCC GCCATGATGG TCTGCCACCG CCGCCGCAAC TGCACCACCA CCACAACCAC 
CACGGTGAAG ACACTGCCTC ACCCACCAAG CCGAGCAGCA AGCTGCCGGC TGGCGGTAAT 
GCCACTTCAC CATCCAACAC GAGGATACTC ACGGGCGTCG GAGGTTCCGG AACTGATGAC 
GGAACCCTAC TGACCACAAA GAGCTCACCC AACCGCCCAC CGGGCACTGC GGCCAGTGGA 
TCCGTTGTCC CCGGGAACGG CAGCGTGGTG CGCACCATCA ACAATATTAA TTTGAACGCA 
GCCGGGATGT CCGGAGGCGA TGATGAGTCC AAGTTGTTTT GCGGCCCCAC TCATGCCCGC 
AATTTGTACT GGAACATGAC TCGAGTGGGT GATGTGAATG TTCAGCCCTG TCCTGGCGGA 
GCAGCCGGCA TCGCCAAGTG GCGTTGCGTT CTAATGAAGA GGATACCCGA CTCCGGCTAC 
GATGAGTACG ATGATGACAT CAGTTCGACA ACTCCGGCAC CCAGCGGTGG CGACTGTCTG 
CACAACAGCA GCAGCTGCGA GCCGCCGGTG AGCATGGCCC ACAAGGTAAA CCAGCGTCTG 
CGCAACTTTG AGCCCACCTG GCATCCCGCG ACACCTGATC TGACGCAATG CCGCAGCCTT 
TGGCTCAACA ATCTGGAAAT GCGAGTAAAC CAGCGGGACT CCTCCTTGAT CTCCATCGCC 
AACGATATGT CCGAAGTGAC CAGTAGCAAA ACGCTCTACG GCGGCGACAT GTTGGTCACC 
ACGAAGATTA TCCAAACAGT GTCCGAGAAG ATGATGCACG ACAAGGAGAC CTTCCCGGAT 
CAGCGACAGC GCGAGGCTAT GATCATGGAG TTGTTGCATT GTGTGGTCAA AACCGGCTCC 
AACCTGCTGG ACGAATCGCA GCTGTCCTCG TGGTTGGATC TCAATCCGGA GGACCAAATG 
CGTGTAGCCA CATCCTTGCT AACTGGCCTG GAATACAATG CCTTTCTGCT GGCGGATACG 
ATCATCAGGG AGCGCAGCGT GGTGCAAAAA GTCAAAAATA TATTGCTCTC CGTTCGAGTT 
CTGGAAACCA AGACTATCCA GTCCAGCGTG GTCTTCCCAG ATTCGGATCA GTGGCCCTTG 
AGTTCGGATC GTATTGAGCT GCCACGAGCT GCTCTAATAG ATAATAGTGA AGGCGGTCTG 
GTGCGAATTG TATTCGCCGC CTTCGATCGC CTGGAATCCA TTCTAAAGCC CAGCTATGAT 
CACTTCGATC TCAAGAGCTC CCGCAGTTAC GTTCGCAATA CAGCCATCCT GAGCAACGAC 
AGCGATGTCA ACGCGGGGGA GATCCAACAG CGCCTACGCA TCCTGAACAG CAAGGTGATC 
TCGGCCAGCT TGGGCAAGGG GCGTCACATA CAACTCTCCC AGCCCATAAC CCTGACACTG 
AAACATCTGA AGACCGAGAA TGTAACGAAT CCCACCTGCG TGTTCTGGAA CTATATTGAC 
CATGCGTGGT CTGCCAACGG ATGCAGTCTG GAGTCCACTA ACCGCACGCA CAGCGTCTGC 
AGTTGCAACC ACCTGACAAA CTTTGCCATA CTAATGGACG TTGTGGATGA GCACCAGCAT 
TCGTTGTTCA CCATGTTCGA TGGAAACATG CGCATATTCA TCTACATAAG CATCGGCATC 
TGCGTGGTCT TCATAGTTAT CGCCCTGCTA ACGCTGAAGC TGTTCAATGG GGTCTTTGTG 
AAGTCCGCGC GCACCTCGAT CTATACCAGC ATTTACCTTT GCCTCCTGGC CATCGAGCTG 
CTCTTTCTCC TGGGCATTGA ACAGACCGAA ACAAGCATTT TCTGCGGCTT CATTACTATT 
TTCCTACACT GTGCCATCCT ATCGGGCACC GCCTGGTTCT GTTACGAAGC CTTCCATTCG 
TACTCAACGC TCACCTCGGA CGAGCTCCTG CTGGAGGTGG ACCAGACGCC CAAGGTGAAC 
TGCTACTACC TCTTGTCCTA CGGACTGTCG CTGAGCGTGG TGGCCATCTC GCTGGTCATC 
GATCCCAGCA CCTATACCCA AAACGATTAT TGCGTGCTGA TGGAGGCGAA TGCCTTGTTT 
TATGCCACCT TTGTAATACC AGTGCTTGTC TTCTTTGTGG CTGCCATTGG TTACACATTC 
CTCTCCTGGA TTATAATGTG CCGCAAAAGT CGCACGGGTC TAAAGACCAA GGAACATACT 
CGCCTCGCTA GCGTGCGGTT CGACATACGC TGCTCCTTTG TGTTCCTCTT GCTGCTCAGC 
GCTGTTTGGT GCTCGGCCTA CTTCTATTTG CGAGGAGCCA AAATGGACGA TGACACGGCT 
GATGTGTATG GATACTGCTT CATCTGCTTC AACACATTGC TGGGGCTCTA TATCTTCGTG 
TTCCATTGCA TTCAAAACGA AAAGATCCGG CGGGAGTATC GGAAGTATGT GAGACAGCAC 
GCTTGGCTGC CCAAGTGCTT GCGCTGCTCG AAAACATCAA TTTCCTCGGG CATTGTTACC 
GGCAATGGAC CCACAGCCGG AACCCTTTGC AGCGTCTCCA CGTCCAAGAA GCCCAAGCTG 
CCGTTAGGAG TGAGCGAAGA GGCGCATGAC GATCCCCAGC AGCAACAGCA GACACCAGTG 
CCCATCACAG AGGATGCCAT TATGGGAGCC ACCTCTGATT GTGAACTGAA CGAGGCCCAG 
CAAAGAAGAA CCCTAAAAAG TGGCCTAATG ACGGGCACAC TACAGGCTCC ACCGCAGACC 
CTTGGTGGCC ATGTTGTGCT CGAAAGAGGT AGCACTCTCC GCTCCACTGG TCATGCCTCA 
CCCACCAGCT CTGCCGGGTC CACACACCTG ATTTTTGCGC ACAAGCAACA ACAACAACAG 
CAGCAACAGG GACCTTTGGG CGAGTCTTAC TACCATCAGC CGGACTACTA CAGCTGGAAG 
CAACCATCAA CTGGAACAGG AGGATTGAAA ACACCGCGGG AGTACTACAA TAATGCGGGT 
GCTGCTGCAT CATCGCCGCA ACAGGCGCAC GAGGTATTCT ACTGGACTCA AAAGCCGAAC 
AGCGGCCACA ATGGCAAAAA GAAGAGGGGC GCCGGAGGAG TTCCCGCCTC GCCTAGTGGA 
TCTCTGCACA GTCGCACGGC CGCCGCCTCC CAGGTGCTTT TCTATCCATC GTACAAGAAG 
ACCAAGCCTG GCCAGCCAAC AGGCTATCCG CAATACGCGG AGGCGTTGGA CCCACCACTA 
GCCACTGGCA ATGCGGCTGC CTACTACCAG CAGCAGCAAC AGTTGCGTCG CCAGCAGCTA 
CATCAGCAGC AGCAACAGCA GCAGCAGCAG CAACTCTCCT CGGACGAGGA GCAGGCCGAG 
CAACATGCTC ACCTGTTGCA CCTGCAACGA CGAGCTGGTA GCCAGCAGCA GCTCCCTGCT 
CCACCGCCAC ACATGGCGCA GTACCAGCAG GAGTTTATGC AGCGCCAGTA TAGAAATAAG 
CATTCCAACT GTGATCTGGG CATGGGCGAT GCCTACTACA ACCAAGGCAG CGTCGGCGGC 
GCGGATGGTG GGCCGGTCTA CGAGGAGATC CTCAGCAACC GCAACTCGGA TGTGCAGCAT 
TACGAGGTGG GTGACTTCGA TGTGGACGAG GTGTACAACA ATAGCGTTGG CACTGGCGTC 
TTCAACAACA TGAGAGCGGC GGTGGCCGCC GGCGGTAGTC GGTACGGCGG CGGAAGCCTG 
AGTGGCGGCA GTGTCTCGTC CAGGAGCCAA CAGCAGCAGC TCAAGAAGCA GCAGCAACAA 
CAGTCGCTGG CTCAGCAAAG ATCGGCTCGA CGCTGCACGG CGGATGACGA TGACGACGAA 
GACGAGGAGG AGGATGAGGA GGCAACGGCC GCGGAGCAAT TGCACGACAG CGTCTGTGAT 
GAGGATGAGG AGGAGGACGA GAGCGACTTG GAGGATGATG CTCATGGATT GCCACCGCAG 
AGCGATGAGC GGATGCGTCG TCTGATGGCG ATGCAGGACG AGGATTTTAA GCGGCGGTTT 
CAACGTCAGC TGCGCAAACA TGGAGCGCCT CTTGACTACG GGGCTTTGCC ACCAGGAGCA 
GGACCGCAAC CCGAGCACAA CGGTGCGGTT TTTGGGGTTA GCGGCGGCGT TGGTGAGGGC 
TCCCTGCGTG GCGCATTCCG GCAGCAGCAG CAGCAAGCAC TGAATGCCAA GTCGCCAGGC 
GGCCGTTTGG CGGTAAATGA GCTATTCGGT CACGGCAACT CGGGACCACC GCTGCCGCCG 
GCGAATCAGA CGCCCGCGCA GAAGCGCCAG CAGCTACAGA AACTGTCACC ACAGTCCACC 
ACATCATCGT CGTCGCATAC ATCACACAGC AATCCCAATC CGCATCCCCA CCAGCTAACC 
CATCCGCATC CCCATCAGCA TCCTCCGCAC CATCAGCAGC GCCACCTGTC GGCCATGCTC 
GATGAGAACA ACACGGTGCG ATGTTATCTG GAACCACTGG CTAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610397.4
CDS	788..5875
Translation	MSKYQTAYACEGKKLTIECDPGDVINLIRANYGRFSITICNDHGNVEWSVNCMFPKSLSVLNSRCAHKQSCGVLAATSMFGDPCPGTHKYLEAHYQCISAAQTSTTTNRPSPPPWVLSNGPPIFGNGSGLIHPPGVGAGAPPPPRLPTLPGVVGISGNPGLFNVPPQHTAVTHSTPSSSTTAVGGGRLKGGATSTTTTKHPAGRHDGLPPPPQLHHHHNHHGEDTASPTKPSSKLPAGGNATSPSNTRILTGVGGSGTDDGTLLTTKSSPNRPPGTAASGSVVPGNGSVVRTINNINLNAAGMSGGDDESKLFCGPTHARNLYWNMTRVGDVNVQPCPGGAAGIAKWRCVLMKRIPDSGYDEYDDDISSTTPAPSGGDCLHNSSSCEPPVSMAHKVNQRLRNFEPTWHPATPDLTQCRSLWLNNLEMRVNQRDSSLISIANDMSEVTSSKTLYGGDMLVTTKIIQTVSEKMMHDKETFPDQRQREAMIMELLHCVVKTGSNLLDESQLSSWLDLNPEDQMRVATSLLTGLEYNAFLLADTIIRERSVVQKVKNILLSVRVLETKTIQSSVVFPDSDQWPLSSDRIELPRAALIDNSEGGLVRIVFAAFDRLESILKPSYDHFDLKSSRSYVRNTAILSNDSDVNAGEIQQRLRILNSKVISASLGKGRHIQLSQPITLTLKHLKTENVTNPTCVFWNYIDHAWSANGCSLESTNRTHSVCSCNHLTNFAILMDVVDEHQHSLFTMFDGNMRIFIYISIGICVVFIVIALLTLKLFNGVFVKSARTSIYTSIYLCLLAIELLFLLGIEQTETSIFCGFITIFLHCAILSGTAWFCYEAFHSYSTLTSDELLLEVDQTPKVNCYYLLSYGLSLSVVAISLVIDPSTYTQNDYCVLMEANALFYATFVIPVLVFFVAAIGYTFLSWIIMCRKSRTGLKTKEHTRLASVRFDIRCSFVFLLLLSAVWCSAYFYLRGAKMDDDTADVYGYCFICFNTLLGLYIFVFHCIQNEKIRREYRKYVRQHAWLPKCLRCSKTSISSGIVTGNGPTAGTLCSVSTSKKPKLPLGVSEEAHDDPQQQQQTPVPITEDAIMGATSDCELNEAQQRRTLKSGLMTGTLQAPPQTLGGHVVLERGSTLRSTGHASPTSSAGSTHLIFAHKQQQQQQQQGPLGESYYHQPDYYSWKQPSTGTGGLKTPREYYNNAGAAASSPQQAHEVFYWTQKPNSGHNGKKKRGAGGVPASPSGSLHSRTAAASQVLFYPSYKKTKPGQPTGYPQYAEALDPPLATGNAAAYYQQQQQLRRQQLHQQQQQQQQQQLSSDEEQAEQHAHLLHLQRRAGSQQQLPAPPPHMAQYQQEFMQRQYRNKHSNCDLGMGDAYYNQGSVGGADGGPVYEEILSNRNSDVQHYEVGDFDVDEVYNNSVGTGVFNNMRAAVAAGGSRYGGGSLSGGSVSSRSQQQQLKKQQQQQSLAQQRSARRCTADDDDDEDEEEDEEATAAEQLHDSVCDEDEEEDESDLEDDAHGLPPQSDERMRRLMAMQDEDFKRRFQRQLRKHGAPLDYGALPPGAGPQPEHNGAVFGVSGGVGEGSLRGAFRQQQQQALNAKSPGGRLAVNELFGHGNSGPPLPPANQTPAQKRQQLQKLSPQSTTSSSSHTSHSNPNPHPHQLTHPHPHQHPPHHQQRHLSAMLDENNTVRCYLEPLAK

Target ORF information:

RefSeq Version	NM_136553.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Calcium-independent receptor for alpha-latrotoxin (Cirl), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136553.5

ORF Insert Sequence:

ATGTCCAAGT ACCAAACCGC CTACGCCTGC GAAGGTAAGA AACTGACCAT CGAGTGCGAT 
CCCGGCGATG TGATCAACCT CATTCGGGCC AACTATGGCC GCTTCTCGAT TACCATCTGC 
AATGACCACG GGAATGTGGA GTGGAGTGTT AACTGCATGT TTCCCAAGTC ACTCAGCGTA 
CTGAACTCAA GATGTGCCCA CAAGCAGAGC TGCGGCGTGT TGGCAGCCAC GAGCATGTTC 
GGGGATCCCT GTCCCGGTAC CCACAAGTAT CTGGAGGCAC ACTACCAGTG CATAAGTGCA 
GCCCAAACTT CGACGACGAC CAACAGGCCC AGTCCGCCGC CATGGGTGCT GAGCAATGGT 
CCGCCGATCT TTGGCAACGG CAGTGGACTG ATCCATCCGC CCGGGGTTGG AGCGGGTGCG 
CCGCCCCCGC CGAGACTTCC CACACTTCCC GGAGTGGTGG GAATCAGTGG GAATCCCGGC 
CTGTTCAACG TACCACCGCA ACACACCGCC GTCACGCACT CCACGCCCTC GAGCAGCACG 
ACAGCCGTGG GCGGTGGACG TTTGAAGGGT GGGGCCACCT CCACGACGAC CACCAAGCAT 
CCGGCTGGCC GCCATGATGG TCTGCCACCG CCGCCGCAAC TGCACCACCA CCACAACCAC 
CACGGTGAAG ACACTGCCTC ACCCACCAAG CCGAGCAGCA AGCTGCCGGC TGGCGGTAAT 
GCCACTTCAC CATCCAACAC GAGGATACTC ACGGGCGTCG GAGGTTCCGG AACTGATGAC 
GGAACCCTAC TGACCACAAA GAGCTCACCC AACCGCCCAC CGGGCACTGC GGCCAGTGGA 
TCCGTTGTCC CCGGGAACGG CAGCGTGGTG CGCACCATCA ACAATATTAA TTTGAACGCA 
GCCGGGATGT CCGGAGGCGA TGATGAGTCC AAGTTGTTTT GCGGCCCCAC TCATGCCCGC 
AATTTGTACT GGAACATGAC TCGAGTGGGT GATGTGAATG TTCAGCCCTG TCCTGGCGGA 
GCAGCCGGCA TCGCCAAGTG GCGTTGCGTT CTAATGAAGA GGATACCCGA CTCCGGCTAC 
GATGAGTACG ATGATGACAT CAGTTCGACA ACTCCGGCAC CCAGCGGTGG CGACTGTCTG 
CACAACAGCA GCAGCTGCGA GCCGCCGGTG AGCATGGCCC ACAAGGTAAA CCAGCGTCTG 
CGCAACTTTG AGCCCACCTG GCATCCCGCG ACACCTGATC TGACGCAATG CCGCAGCCTT 
TGGCTCAACA ATCTGGAAAT GCGAGTAAAC CAGCGGGACT CCTCCTTGAT CTCCATCGCC 
AACGATATGT CCGAAGTGAC CAGTAGCAAA ACGCTCTACG GCGGCGACAT GTTGGTCACC 
ACGAAGATTA TCCAAACAGT GTCCGAGAAG ATGATGCACG ACAAGGAGAC CTTCCCGGAT 
CAGCGACAGC GCGAGGCTAT GATCATGGAG TTGTTGCATT GTGTGGTCAA AACCGGCTCC 
AACCTGCTGG ACGAATCGCA GCTGTCCTCG TGGTTGGATC TCAATCCGGA GGACCAAATG 
CGTGTAGCCA CATCCTTGCT AACTGGCCTG GAATACAATG CCTTTCTGCT GGCGGATACG 
ATCATCAGGG AGCGCAGCGT GGTGCAAAAA GTCAAAAATA TATTGCTCTC CGTTCGAGTT 
CTGGAAACCA AGACTATCCA GTCCAGCGTG GTCTTCCCAG ATTCGGATCA GTGGCCCTTG 
AGTTCGGATC GTATTGAGCT GCCACGAGCT GCTCTAATAG ATAATAGTGA AGGCGGTCTG 
GTGCGAATTG TATTCGCCGC CTTCGATCGC CTGGAATCCA TTCTAAAGCC CAGCTATGAT 
CACTTCGATC TCAAGAGCTC CCGCAGTTAC GTTCGCAATA CAGCCATCCT GAGCAACGAC 
AGCGATGTCA ACGCGGGGGA GATCCAACAG CGCCTACGCA TCCTGAACAG CAAGGTGATC 
TCGGCCAGCT TGGGCAAGGG GCGTCACATA CAACTCTCCC AGCCCATAAC CCTGACACTG 
AAACATCTGA AGACCGAGAA TGTAACGAAT CCCACCTGCG TGTTCTGGAA CTATATTGAC 
CATGCGTGGT CTGCCAACGG ATGCAGTCTG GAGTCCACTA ACCGCACGCA CAGCGTCTGC 
AGTTGCAACC ACCTGACAAA CTTTGCCATA CTAATGGACG TTGTGGATGA GCACCAGCAT 
TCGTTGTTCA CCATGTTCGA TGGAAACATG CGCATATTCA TCTACATAAG CATCGGCATC 
TGCGTGGTCT TCATAGTTAT CGCCCTGCTA ACGCTGAAGC TGTTCAATGG GGTCTTTGTG 
AAGTCCGCGC GCACCTCGAT CTATACCAGC ATTTACCTTT GCCTCCTGGC CATCGAGCTG 
CTCTTTCTCC TGGGCATTGA ACAGACCGAA ACAAGCATTT TCTGCGGCTT CATTACTATT 
TTCCTACACT GTGCCATCCT ATCGGGCACC GCCTGGTTCT GTTACGAAGC CTTCCATTCG 
TACTCAACGC TCACCTCGGA CGAGCTCCTG CTGGAGGTGG ACCAGACGCC CAAGGTGAAC 
TGCTACTACC TCTTGTCCTA CGGACTGTCG CTGAGCGTGG TGGCCATCTC GCTGGTCATC 
GATCCCAGCA CCTATACCCA AAACGATTAT TGCGTGCTGA TGGAGGCGAA TGCCTTGTTT 
TATGCCACCT TTGTAATACC AGTGCTTGTC TTCTTTGTGG CTGCCATTGG TTACACATTC 
CTCTCCTGGA TTATAATGTG CCGCAAAAGT CGCACGGGTC TAAAGACCAA GGAACATACT 
CGCCTCGCTA GCGTGCGGTT CGACATACGC TGCTCCTTTG TGTTCCTCTT GCTGCTCAGC 
GCTGTTTGGT GCTCGGCCTA CTTCTATTTG CGAGGAGCCA AAATGGACGA TGACACGGCT 
GATGTGTATG GATACTGCTT CATCTGCTTC AACACATTGC TGGGGCTCTA TATCTTCGTG 
TTCCATTGCA TTCAAAACGA AAAGATCCGG CGGGAGTATC GGAAGTATGT GAGACAGCAC 
GCTTGGCTGC CCAAGTGCTT GCGCTGCTCG AAAACATCAA TTTCCTCGGG CATTGTTACC 
GGCAATGGAC CCACAGCCGG AACCCTTTGC AGCGTCTCCA CGTCCAAGAA GCCCAAGCTG 
CCGTTAGGAG TGAGCGAAGA GGCGCATGAC GATCCCCAGC AGCAACAGCA GACACCAGTG 
CCCATCACAG AGGATGCCAT TATGGGAGCC ACCTCTGATT GTGAACTGAA CGAGGCCCAG 
CAAAGAAGAA CCCTAAAAAG TGGCCTAATG ACGGGCACAC TACAGGCTCC ACCGCAGACC 
CTTGGTGGCC ATGTTGTGCT CGAAAGAGGT AGCACTCTCC GCTCCACTGG TCATGCCTCA 
CCCACCAGCT CTGCCGGGTC CACACACCTG ATTTTTGCGC ACAAGCAACA ACAACAACAG 
CAGCAACAGG GACCTTTGGG CGAGTCTTAC TACCATCAGC CGGACTACTA CAGCTGGAAG 
CAACCATCAA CTGGAACAGG AGGATTGAAA ACACCGCGGG AGTACTACAA TAATGCGGGT 
GCTGCTGCAT CATCGCCGCA ACAGGCGCAC GAGGTATTCT ACTGGACTCA AAAGCCGAAC 
AGCGGCCACA ATGGCAAAAA GAAGAGGGGC GCCGGAGGAG TTCCCGCCTC GCCTAGTGGA 
TCTCTGCACA GTCGCACGGC CGCCGCCTCC CAGGTGCTTT TCTATCCATC GTACAAGAAG 
ACCAAGCCTG GCCAGCCAAC AGGCTATCCG CAATACGCGG AGGCGTTGGA CCCACCACTA 
GCCACTGGCA ATGCGGCTGC CTACTACCAG CAGCAGCAAC AGTTGCGTCG CCAGCAGCTA 
CATCAGCAGC AGCAACAGCA GCAGCAGCAG CAACTCTCCT CGGACGAGGA GCAGGCCGAG 
CAACATGCTC ACCTGTTGCA CCTGCAACGA CGAGCTGGTA GCCAGCAGCA GCTCCCTGCT 
CCACCGCCAC ACATGGCGCA GTACCAGCAG GAGTTTATGC AGCGCCAGTA TAGAAATAAG 
CATTCCAACT GTGATCTGGG CATGGGCGAT GCCTACTACA ACCAAGGCAG CGTCGGCGGC 
GCGGATGGTG GGCCGGTCTA CGAGGAGATC CTCAGCAACC GCAACTCGGA TGTGCAGCAT 
TACGAGGTGG GTGACTTCGA TGTGGACGAG GTGTACAACA ATAGCGTTGG CACTGGCGTC 
TTCAACAACA TGAGAGCGGC GGTGGCCGCC GGCGGTAGTC GGTACGGCGG CGGAAGCCTG 
AGTGGCGGCA GTGTCTCGTC CAGGAGCCAA CAGCAGCAGC TCAAGAAGCA GCAGCAACAA 
CAGTCGCTGG CTCAGCAAAG ATCGGCTCGA CGCTGCACGG CGGATGACGA TGACGACGAA 
GACGAGGAGG AGGATGAGGA GGCAACGGCC GCGGAGCAAT TGCACGACAG CGTCTGTGAT 
GAGGATGAGG AGGAGGACGA GAGCGACTTG GAGGATGATG CTCATGGATT GCCACCGCAG 
AGCGATGAGC GGATGCGTCG TCTGATGGCG ATGCAGGACG AGGATTTTAA GCGGCGGTTT 
CAACGTCAGC TGCGCAAACA TGGAGCGCCT CTTGACTACG GGGCTTTGCC ACCAGGAGCA 
GGACCGCAAC CCGAGCACAA CGGTGCGGTT TTTGGGGTTA GCGGCGGCGT TGGTGAGGGC 
TCCCTGCGTG GCGCATTCCG GCAGCAGCAG CAGCAAGCAC TGAATGCCAA GTCGCCAGGC 
GGCCGTTTGG CGGTAAATGA GCTATTCGGT CACGGCAACT CGGGACCACC GCTGCCGCCG 
GCGAATCAGA CGCCCGCGCA GAAGCGCCAG CAGCTACAGA AACTGTCACC ACAGTCCACC 
ACATCATCGT CGTCGCATAC ATCACACAGC AATCCCAATC CGCATCCCCA CCAGCTAACC 
CATCCGCATC CCCATCAGCA TCCTCCGCAC CATCAGCAGC GCCACCTGTC GGCCATGCTC 
GATGAGAACA ACACGGTGCG ATGTTATCTG GAACCACTGG CTAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Cirl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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