mtt cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	mtt
Entrez Gene ID	35832
Full Name	mangetout
Synonyms	CG18447,CG30361,CG8692,DmGluBR,DmGluRB,DmXR,Dmel\CG30361,Glu-RB,GluRB,anon-WO0170980.4,anon-WO0170980.5,mXr
General protein information	Preferred Names mangetout Names CG30361 gene product from transcript CG30361-RE CG30361-PC CG30361-PD CG30361-PE CG30361-PF CG30361-PG DmX receptor mXr mtt-PC mtt-PD mtt-PE mtt-PF mtt-PG
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 44D1-44D2

mRNA and Protein(s)

mRNA	Protein	Name
NM_136542.3	NP_610386.2	mangetout, isoform E
NM_206058.2	NP_995780.1	mangetout, isoform C
NM_001144147.2	NP_001137619.1	mangetout, isoform D
NM_165609.3	NP_724683.2	mangetout, isoform F
NM_001273877.1	NP_001260806.1	mangetout, isoform G

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC569768	XP_001922055.2
Bos taurus (cattle)	GRM8	NP_001193046.1
Drosophila melanogaster (fruit fly)	mtt	NP_610386.2
Homo sapiens (human)	GRM8	NP_000836.2
	GRM8	XP_001088843.2
Canis lupus familiaris (dog)	GRM8	XP_005628488.1
Rattus norvegicus (Norway rat)	Grm8	NP_071538.1
Gallus gallus (chicken)	GRM8	XP_425426.3
Pan troglodytes (chimpanzee)	GRM8	XP_527877.2
Mus musculus (house mouse)	Grm8	NP_032200.2
Anopheles gambiae (African malaria mosquito)	GPRMGL5	XP_313901.4
Xenopus tropicalis (tropical clawed frog)	LOC100497088	XP_004913052.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mtt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mtt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa12775	NM_206058.2 Latest version!	Drosophila melanogaster mangetout (mtt), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa09294	NM_001273877.1 Latest version!	Drosophila melanogaster mangetout (mtt), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$797.30 $1139.00
OFa12773	NM_001144147.2 Latest version!	Drosophila melanogaster mangetout (mtt), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$797.30 $1139.00
OFa12772	NM_136542.3 Latest version!	Drosophila melanogaster mangetout (mtt), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa12774	NM_165609.3 Latest version!	Drosophila melanogaster mangetout (mtt), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mtt ( NM_206058.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995780.1 Drosophila melanogaster mangetout (mtt), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa12775
Clone ID Related Accession (Same CDS sequence)	NM_206058.2
Accession Version	NM_206058.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mangetout, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_206058.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGGGCGG TGCGCCGGGC GAATGCAACT GGTTCCTTCT CCTGGATCGG ATCCGATGGC 
TGGAGTGCCC GGAATTTGGT GTCCGACGAC TACGAGCCGG AGGTGGAAGG CACGCTGTCT 
GTGCAGCCGC AGGCGAATCC GGTGCGCGGG TTCGAGGAGT ACTTCCTCAG TCTGACGGTG 
GAGAACAATC AACGCAATCC CTGGTTTGTG GAATTCTGGG AGGATCACTT TCAGTGCCGA 
TATCCCGGCA GTACGAGCAC TCCGTACAAT AACTACACCA AACAGTGCAC CACAAAGGAG 
CGACTTTCAC GCCAGAACAC TGACTTCGAG GACCAGTTGC AGTTTGTCAG CGATGCGGTG 
ATGGCATTTG CATACGCCCT AAGGGATATG CACCGCGACC TGTGCGGCGG AGGTCCTTCG 
CTCTGCGAGG CCATGAAGCC AACGAAGGGT GCAGACTTGC TGAAATATTT GCGAAAAGTG 
GAGTTTGAGG GCCTCAGCGG CGACGAGTTC CGCTTCGACG GGAACGGCGA CGGTCCCGCC 
CGGTACAACA TCATCCACTT CAAGCAGTCC CAGGCGGGAC AGTACCACTG GGTCAAGGTG 
GGCGAGTACA CCGAGGGCGA GCTGCGGCTT AATATGACGG AGGTGAAGTT TAAGCGGCTG 
AGTCCCAAGC CGCCGGAGTC TGTCTGCAGC CTGCCATGTC TTGTGGGTCA GGCCAAGAAG 
TACGTGGAAG GCGAGAGCTG CTGTTGGCAC TGCTTCAATT GCACAACCTA TCAAATCCGC 
CACCCGGATG ACGAGACCCA TTGCAAGCTG TGCAAGCTGG GCACTCTTCC CGACGCCCAC 
AAGCAGTACT GCCGCCCCAT CCCGGAGATA TATTTGCGTC CGGAGTCCGC TTGGGCCATC 
GGTGCTATGG CGTTCAGTGC GACCGGGATC TTAGTAACGC TCTTCGTAAT GGGCGTATTT 
GTCAGGCACA ACGACACGCC CATAGTGCGT GCCTCTGGTC GGGAGCTGAG CTACATCCTG 
CTGGCGGGGA TTTTCATGTG CTACGGTGTC ACCTTTGCCC TGGTCCTGAA GCCGACCAAC 
ATAGTGTGTG CCATTCAGCG GTTTGGTGTC GGCTTCTGCT TCACGGTCGT TTATGCAGCA 
CTGTTGACCA AAACGAATCG CATTGCACGG ATCTTCAAAG CGGGCAAACA ATCCGCCAAG 
AGGCCCTCCT TCATTAGTCC CAAGTCCCAG CTGGTAATCT GTGCGTGCCT GGTTAGTGTG 
CAGATACTCA TCAACGGCGT TTGGATGGTA ATTGCGCCAT CACATGCCAT GCACCATTAT 
CCCACACGCG AGGATAATCT GCTCGTCTGC GACTCGTACA TCGACGCCTC CTACATGATT 
GCGTTCTCCT ACCCAATCTT CCTGATTGTT ATTTGCACGG TCTACGCGGT GCTCACGCGA 
AAGATTCCGG AGGCTTTCAA CGAATCGAAG CATATTGGAT TCACCATGTA CACGACTTGT 
GTGATTTGGC TGGCTTTTGT ACCGCTCTAC TTCGGAACCG CCAACCACGT TCCGCTGCGC 
ATCACCAGCA TGTCGGTGAC CATCAGCTTA TCCGCCAGCG TGACCATCGC CTGTCTCTTT 
TCGCCCAAGC TGTACATAAT ACTCATACGC CCCGAGCGCA ACGTGCGCCA GAGTATGATG 
CCGCCTCGCT ACGGCAACAT GCACCGCACC GCTGGCACAG GACCCTCGTC CATGATGGCC 
GCCGCCGTGG TGACCGCCGC CACCTGTGCC CAGGAGGAGA AGATCCAGAA GCATATCACT 
CCCACAAACA CAGAAAACTC GCTAAAGACG AAGAAATTGT GCGAAATGGC CACTCAGACA 
ATTTCCAGTA TAATCACGTC GCTGGACATC AATGCGTACA ATCAAATACC ATATGCCGAT 
CAATATGTGC CAAACAATGC CACAACGGCC ACGACCACGC CCACAGATAA GGGCAGTGGC 
AACGGCAACA GCAACAGCAA TAGCAATACG AATGGTAGTA GCAATAGCAA CAGCAACAGC 
AATGGCGATG GCGATGTGGC AACAGAGCAA GTCGTGGCCA ACAATAATAA AATCAATCAG 
CGACAACATG GCCAGCCGGC CGTGGCATTT GCCATCACAT CGACTAACAA TCACATAAGC 
GGCCCAGCAG CAACCATAAC AACAACAGCG GCAACTGGCG AAACAACAAC TGAGGCGCTG 
GTGGCTACAA TAACAACTCC ATTGGCAACG GTGGATGGCA GCGAAACCAT GTCCGTGGCT 
CCCAACAATG GGCATGGAAA CAACGGGAGC CAACGTCCGC CGGTCCTGCA AACAAACTTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995780.1
CDS	93..2435
Translation	MRAVRRANATGSFSWIGSDGWSARNLVSDDYEPEVEGTLSVQPQANPVRGFEEYFLSLTVENNQRNPWFVEFWEDHFQCRYPGSTSTPYNNYTKQCTTKERLSRQNTDFEDQLQFVSDAVMAFAYALRDMHRDLCGGGPSLCEAMKPTKGADLLKYLRKVEFEGLSGDEFRFDGNGDGPARYNIIHFKQSQAGQYHWVKVGEYTEGELRLNMTEVKFKRLSPKPPESVCSLPCLVGQAKKYVEGESCCWHCFNCTTYQIRHPDDETHCKLCKLGTLPDAHKQYCRPIPEIYLRPESAWAIGAMAFSATGILVTLFVMGVFVRHNDTPIVRASGRELSYILLAGIFMCYGVTFALVLKPTNIVCAIQRFGVGFCFTVVYAALLTKTNRIARIFKAGKQSAKRPSFISPKSQLVICACLVSVQILINGVWMVIAPSHAMHHYPTREDNLLVCDSYIDASYMIAFSYPIFLIVICTVYAVLTRKIPEAFNESKHIGFTMYTTCVIWLAFVPLYFGTANHVPLRITSMSVTISLSASVTIACLFSPKLYIILIRPERNVRQSMMPPRYGNMHRTAGTGPSSMMAAAVVTAATCAQEEKIQKHITPTNTENSLKTKKLCEMATQTISSIITSLDINAYNQIPYADQYVPNNATTATTTPTDKGSGNGNSNSNSNTNGSSNSNSNSNGDGDVATEQVVANNNKINQRQHGQPAVAFAITSTNNHISGPAATITTTAATGETTTEALVATITTPLATVDGSETMSVAPNNGHGNNGSQRPPVLQTNL

Target ORF information:

RefSeq Version	NM_206058.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mangetout (mtt), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206058.2

ORF Insert Sequence:

ATGCGGGCGG TGCGCCGGGC GAATGCAACT GGTTCCTTCT CCTGGATCGG ATCCGATGGC 
TGGAGTGCCC GGAATTTGGT GTCCGACGAC TACGAGCCGG AGGTGGAAGG CACGCTGTCT 
GTGCAGCCGC AGGCGAATCC GGTGCGCGGG TTCGAGGAGT ACTTCCTCAG TCTGACGGTG 
GAGAACAATC AACGCAATCC CTGGTTTGTG GAATTCTGGG AGGATCACTT TCAGTGCCGA 
TATCCCGGCA GTACGAGCAC TCCGTACAAT AACTACACCA AACAGTGCAC CACAAAGGAG 
CGACTTTCAC GCCAGAACAC TGACTTCGAG GACCAGTTGC AGTTTGTCAG CGATGCGGTG 
ATGGCATTTG CATACGCCCT AAGGGATATG CACCGCGACC TGTGCGGCGG AGGTCCTTCG 
CTCTGCGAGG CCATGAAGCC AACGAAGGGT GCAGACTTGC TGAAATATTT GCGAAAAGTG 
GAGTTTGAGG GCCTCAGCGG CGACGAGTTC CGCTTCGACG GGAACGGCGA CGGTCCCGCC 
CGGTACAACA TCATCCACTT CAAGCAGTCC CAGGCGGGAC AGTACCACTG GGTCAAGGTG 
GGCGAGTACA CCGAGGGCGA GCTGCGGCTT AATATGACGG AGGTGAAGTT TAAGCGGCTG 
AGTCCCAAGC CGCCGGAGTC TGTCTGCAGC CTGCCATGTC TTGTGGGTCA GGCCAAGAAG 
TACGTGGAAG GCGAGAGCTG CTGTTGGCAC TGCTTCAATT GCACAACCTA TCAAATCCGC 
CACCCGGATG ACGAGACCCA TTGCAAGCTG TGCAAGCTGG GCACTCTTCC CGACGCCCAC 
AAGCAGTACT GCCGCCCCAT CCCGGAGATA TATTTGCGTC CGGAGTCCGC TTGGGCCATC 
GGTGCTATGG CGTTCAGTGC GACCGGGATC TTAGTAACGC TCTTCGTAAT GGGCGTATTT 
GTCAGGCACA ACGACACGCC CATAGTGCGT GCCTCTGGTC GGGAGCTGAG CTACATCCTG 
CTGGCGGGGA TTTTCATGTG CTACGGTGTC ACCTTTGCCC TGGTCCTGAA GCCGACCAAC 
ATAGTGTGTG CCATTCAGCG GTTTGGTGTC GGCTTCTGCT TCACGGTCGT TTATGCAGCA 
CTGTTGACCA AAACGAATCG CATTGCACGG ATCTTCAAAG CGGGCAAACA ATCCGCCAAG 
AGGCCCTCCT TCATTAGTCC CAAGTCCCAG CTGGTAATCT GTGCGTGCCT GGTTAGTGTG 
CAGATACTCA TCAACGGCGT TTGGATGGTA ATTGCGCCAT CACATGCCAT GCACCATTAT 
CCCACACGCG AGGATAATCT GCTCGTCTGC GACTCGTACA TCGACGCCTC CTACATGATT 
GCGTTCTCCT ACCCAATCTT CCTGATTGTT ATTTGCACGG TCTACGCGGT GCTCACGCGA 
AAGATTCCGG AGGCTTTCAA CGAATCGAAG CATATTGGAT TCACCATGTA CACGACTTGT 
GTGATTTGGC TGGCTTTTGT ACCGCTCTAC TTCGGAACCG CCAACCACGT TCCGCTGCGC 
ATCACCAGCA TGTCGGTGAC CATCAGCTTA TCCGCCAGCG TGACCATCGC CTGTCTCTTT 
TCGCCCAAGC TGTACATAAT ACTCATACGC CCCGAGCGCA ACGTGCGCCA GAGTATGATG 
CCGCCTCGCT ACGGCAACAT GCACCGCACC GCTGGCACAG GACCCTCGTC CATGATGGCC 
GCCGCCGTGG TGACCGCCGC CACCTGTGCC CAGGAGGAGA AGATCCAGAA GCATATCACT 
CCCACAAACA CAGAAAACTC GCTAAAGACG AAGAAATTGT GCGAAATGGC CACTCAGACA 
ATTTCCAGTA TAATCACGTC GCTGGACATC AATGCGTACA ATCAAATACC ATATGCCGAT 
CAATATGTGC CAAACAATGC CACAACGGCC ACGACCACGC CCACAGATAA GGGCAGTGGC 
AACGGCAACA GCAACAGCAA TAGCAATACG AATGGTAGTA GCAATAGCAA CAGCAACAGC 
AATGGCGATG GCGATGTGGC AACAGAGCAA GTCGTGGCCA ACAATAATAA AATCAATCAG 
CGACAACATG GCCAGCCGGC CGTGGCATTT GCCATCACAT CGACTAACAA TCACATAAGC 
GGCCCAGCAG CAACCATAAC AACAACAGCG GCAACTGGCG AAACAACAAC TGAGGCGCTG 
GTGGCTACAA TAACAACTCC ATTGGCAACG GTGGATGGCA GCGAAACCAT GTCCGTGGCT 
CCCAACAATG GGCATGGAAA CAACGGGAGC CAACGTCCGC CGGTCCTGCA AACAAACTTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mtt ( NM_001273877.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260806.1 Drosophila melanogaster mangetout (mtt), transcript variant G, mRNA.

Price & Availability↑

CloneID	OFa09294
Clone ID Related Accession (Same CDS sequence)	NM_001273877.1
Accession Version	NM_001273877.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mangetout, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAACTG CCAATATTGT GGCAGCGCCA GCAGCACCGA CCATATCGCC GGCAATAACG 
AGCACGGCGA CCAGGATTGG TGGCAAGGCG GTGCCCACGC CTCGGTTACT CGGATCCGGT 
GCGGCGAATG TGGCCCAGAT ACTGGCCAAG TCTCTGGACA GTCCGTCCCC ATCATCGTCA 
TCCGGCTGGC CGGTTAAGCA TGCCGCTGTG CTCGAGGGCG ATGTTATATT GGGCGGCCTG 
ATGATGGTCC ACTCGCGCGA GGACAGCATC ACTTGCGGAC CCATAATGCC GCAGGGCGGC 
ATCCAGGCCT TGGAGGCGAT GCTATACACC TTGGACCAGG TGAACAAGCA ACAGCTGCTG 
CCGAACGTCA CTCTGGGCGC CCACCTGCTC GACGATTGCG ACAAGGACAC CTACGGCCTG 
GAAATGGCAG TCGACTTTAT TAAAGCGCAT ATACAAATAA ATTCAGAATA TATCACGGTA 
AAAACCGAAG CCAAGGCCAA AATGAAAACA TTCTGTGACG AAAGCAAATG GTCCGTGCAA 
ATTTGTTTGC TCCTGCTCCT GGTCGTTCTG TCCACTTGTC ACATCCTGCA GACCGAAAGC 
CAGGTGGTCG GCGACATCGA ACAGGCGGAC GACGGGGTGG CGTCCAGTCA TCGTCGCAGT 
CGTAGTCACA GTGCCAGCCA TCAAGCTCAC GTTGGTAATG GCAATAATCC TGGCTGCAAG 
CACCTCCATC ATCGTCAGCA TCGCGGCAAA AGTCACGCCC GGAAGCACAG ACAGAGCCAT 
TACGAGATGC TCGAGCTGGA GCAGCGGCAG CAGCGGATGG GCAAGCACTG GCGTCAGTGG 
ATGAGCCACC TGCAGTCGCC GGATTATGCC AACGATGACC CATTGCCAGA TGGCAGGCGC 
TCCTCGCCGG CTAATGCAGA CCTTACCTAC GACCACTTCG AGCTGGAGGA CTACCAGCGA 
TATGCTAATA TTGACCTAGC ATCGCCGCGC TCCGGTCGCC ATCGCGGTCG CCATCTGCCC 
CTGAACAACA TGAACCTAAT GCTGCCGCTC AGCTCGGCGG ATGGTAGTGG CAAGTACCTG 
AGATCCCTGC CGGATTCGGT GAGCTTCCAA AACGATCGAT TGTACGCCAC CCAGCCGCAA 
AGGCACTGGC CCGTCAAGCG GGAGGCTATC GTGGAGGGCG ACGTCATTCT GGGCGGTCTG 
ATGATGGTCC ACTCGCGCGA GGACAGCATC ACCTGCGGTC CCATCATGCC GCAGGGTGGC 
ATCCAGGCCC TGGAGGCCAT GCTCTTCACT ATCGACAGGA TAAACCGGGA GCAGCTCCTG 
CCGAACATTA CCCTGGGTGC CCATATCCTC GACGACTGCG ATAAGGACTC GTATGGCCTT 
GAAATGGCCG TGGATTTTAT CAAAGGGTCC ATCAGCAACA TAGATGACGC GGAGTATCAC 
TGCAATAAGA CGCAGGTGCG CAAGGTCATC TCGGGGGTGG TGGGTGCCGC CTCCTCGGTC 
ACATCCATAC AAGTTGCCAA TTTATTGCGG TTATTCCGAA TTCCCCAGGT GTCGTACTTC 
TCCACCAGCC CCGAGCTGAG CAACAAACAG CGATTCGAGT ACTTCTCCCG CACTATTCCC 
TCGGATCACT ACCAGGTGAA GGCCATGGTG GAGATAGTGA AGCGGATGGG ATGGAGCTAC 
GTGTCGATTA TCTACGAGGA GAGCAATTAC GGAATCAAGG CTTTCGAGGA ACTGGAGGAG 
CTCCTCGCAC GCCATAACAT TTGTATTGCC ATCAAGGAGA AGCTGGTGAA GGATTCGGGA 
GTGGCCGAGG ACATCGCCTA CGACAATATA GTGCAGAAGC TGCTGACGAA GCCGCGGGCT 
CGAGGGGCCA TTATCTTCGG TTCGGATCAA GAGGTGCGCC AAGTGATGCG GGCGGTGCGC 
CGGGCGAATG CAACTGGTTC CTTCTCCTGG ATCGGATCCG ATGGCTGGAG TGCCCGGAAT 
TTGGTGTCCG ACGACTACGA GCCGGAGGTG GAAGGCACGC TGTCTGTGCA GCCGCAGGCG 
AATCCGGTGC GCGGGTTCGA GGAGTACTTC CTCAGTCTGA CGGTGGAGAA CAATCAACGC 
AATCCCTGGT TTGTGGAATT CTGGGAGGAT CACTTTCAGT GCCGATATCC CGGCAGTACG 
AGCACTCCGT ACAATAACTA CACCAAACAG TGCACCACAA AGGAGCGACT TTCACGCCAG 
AACACTGACT TCGAGGACCA GTTGCAGTTT GTCAGCGATG CGGTGATGGC ATTTGCATAC 
GCCCTAAGGG ATATGCACCG CGACCTGTGC GGCGGAGGTC CTTCGCTCTG CGAGGCCATG 
AAGCCAACGA AGGGTGCAGA CTTGCTGAAA TATTTGCGAA AAGTGGAGTT TGAGGGCCTC 
AGCGGCGACG AGTTCCGCTT CGACGGGAAC GGCGACGGTC CCGCCCGGTA CAACATCATC 
CACTTCAAGC AGTCCCAGGC GGGACAGTAC CACTGGGTCA AGGTGGGCGA GTACACCGAG 
GGCGAGCTGC GGCTTAATAT GACGGAGGTG AAGTTTAAGC GGCTGAGTCC CAAGCCGCCG 
GAGTCTGTCT GCAGCCTGCC ATGTCTTGTG GGTCAGGCCA AGAAGTACGT GGAAGGCGAG 
AGCTGCTGTT GGCACTGCTT CAATTGCACA ACCTATCAAA TCCGCCACCC GGATGACGAG 
ACCCATTGCA AGCTGTGCAA GCTGGGCACT CTTCCCGACG CCCACAAGCA GTACTGCCGC 
CCCATCCCGG AGATATATTT GCGTCCGGAG TCCGCTTGGG CCATCGGTGC TATGGCGTTC 
AGTGCGACCG GGATCTTAGT AACGCTCTTC GTAATGGGCG TATTTGTCAG GCACAACGAC 
ACGCCCATAG TGCGTGCCTC TGGTCGGGAG CTGAGCTACA TCCTGCTGGC GGGGATTTTC 
ATGTGCTACG GTGTCACCTT TGCCCTGGTC CTGAAGCCGA CCAACATAGT GTGTGCCATT 
CAGCGGTTTG GTGTCGGCTT CTGCTTCACG GTCGTTTATG CAGCACTGTT GACCAAAACG 
AATCGCATTG CACGGATCTT CAAAGCGGGC AAACAATCCG CCAAGAGGCC CTCCTTCATT 
AGTCCCAAGT CCCAGCTGGT AATCTGTGCG TGCCTGGTTA GTGTGCAGAT ACTCATCAAC 
GGCGTTTGGA TGGTAATTGC GCCATCACAT GCCATGCACC ATTATCCCAC ACGCGAGGAT 
AATCTGCTCG TCTGCGACTC GTACATCGAC GCCTCCTACA TGATTGCGTT CTCCTACCCA 
ATCTTCCTGA TTGTTATTTG CACGGTCTAC GCGGTGCTCA CGCGAAAGAT TCCGGAGGCT 
TTCAACGAAT CGAAGCATAT TGGATTCACC ATGTACACGA CTTGTGTGAT TTGGCTGGCT 
TTTGTACCGC TCTACTTCGG AACCGCCAAC CACGTTCCGC TGCGCATCAC CAGCATGTCG 
GTGACCATCA GCTTATCCGC CAGCGTGACC ATCGCCTGTC TCTTTTCGCC CAAGCTGTAC 
ATAATACTCA TACGCCCCGA GCGCAACGTG CGCCAGAGTA TGATGCCGCC TCGCTACGGC 
AACATGCACC GCACCGCTGG CACAGGACCC TCGTCCATGA TGGCCGCCGC CGTGGTGACC 
GCCGCCACCT GTGCCCAGGA GGAGAAGATC CAGAAGCATA TCACTCCCAC AAACACAGAA 
AACTCGCTAA AGACGAAGAA ATTGTGCGAA ATGGCCACTC AGACAATTTC CAGTATAATC 
ACGTCGCTGG ACATCAATGC GTACAATCAA ATACCATATG CCGATCAATA TGTGCCAAAC 
AATGCCACAA CGGCCACGAC CACGCCCACA GATAAGGGCA GTGGCAACGG CAACAGCAAC 
AGCAATAGCA ATACGAATGG TAGTAGCAAT AGCAACAGCA ACAGCAATGG CGATGGCGAT 
GTGGCAACAG AGCAAGTCGT GGCCAACAAT AATAAAATCA ATCAGCGACA ACATGGCCAG 
CCGGCCGTGG CATTTGCCAT CACATCGACT AACAATCACA TAAGCGGCCC AGCAGCAACC 
ATAACAACAA CAGCGGCAAC TGGCGAAACA ACAACTGAGG CGCTGGTGGC TACAATAACA 
ACTCCATTGG CAACGGTGGA TGGCAGCGAA ACCATGTCCG TGGCTCCCAA CAATGGGCAT 
GGAAACAACG GGAGCCAACG TCCGCCGGTC CTGCAAACAA ACTTATAGCT AAACGCGCAA 
CAATTGCAGC AACAGCTGCT GAAGGAGCAG CAGCGACAAC AAGCCGAGCT GCAAACGCAA 
CATCCGCAGC AGCAACATGG AACACAAAGC TCTTCCAGCG ACACCGCCAG CACGGGCAGC 
GGCAGTGCGG CGAAGAAGCG GGCCGCAATT CCCGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260806.1
CDS	1622..6040
Translation	MTTANIVAAPAAPTISPAITSTATRIGGKAVPTPRLLGSGAANVAQILAKSLDSPSPSSSSGWPVKHAAVLEGDVILGGLMMVHSREDSITCGPIMPQGGIQALEAMLYTLDQVNKQQLLPNVTLGAHLLDDCDKDTYGLEMAVDFIKAHIQINSEYITVKTEAKAKMKTFCDESKWSVQICLLLLLVVLSTCHILQTESQVVGDIEQADDGVASSHRRSRSHSASHQAHVGNGNNPGCKHLHHRQHRGKSHARKHRQSHYEMLELEQRQQRMGKHWRQWMSHLQSPDYANDDPLPDGRRSSPANADLTYDHFELEDYQRYANIDLASPRSGRHRGRHLPLNNMNLMLPLSSADGSGKYLRSLPDSVSFQNDRLYATQPQRHWPVKREAIVEGDVILGGLMMVHSREDSITCGPIMPQGGIQALEAMLFTIDRINREQLLPNITLGAHILDDCDKDSYGLEMAVDFIKGSISNIDDAEYHCNKTQVRKVISGVVGAASSVTSIQVANLLRLFRIPQVSYFSTSPELSNKQRFEYFSRTIPSDHYQVKAMVEIVKRMGWSYVSIIYEESNYGIKAFEELEELLARHNICIAIKEKLVKDSGVAEDIAYDNIVQKLLTKPRARGAIIFGSDQEVRQVMRAVRRANATGSFSWIGSDGWSARNLVSDDYEPEVEGTLSVQPQANPVRGFEEYFLSLTVENNQRNPWFVEFWEDHFQCRYPGSTSTPYNNYTKQCTTKERLSRQNTDFEDQLQFVSDAVMAFAYALRDMHRDLCGGGPSLCEAMKPTKGADLLKYLRKVEFEGLSGDEFRFDGNGDGPARYNIIHFKQSQAGQYHWVKVGEYTEGELRLNMTEVKFKRLSPKPPESVCSLPCLVGQAKKYVEGESCCWHCFNCTTYQIRHPDDETHCKLCKLGTLPDAHKQYCRPIPEIYLRPESAWAIGAMAFSATGILVTLFVMGVFVRHNDTPIVRASGRELSYILLAGIFMCYGVTFALVLKPTNIVCAIQRFGVGFCFTVVYAALLTKTNRIARIFKAGKQSAKRPSFISPKSQLVICACLVSVQILINGVWMVIAPSHAMHHYPTREDNLLVCDSYIDASYMIAFSYPIFLIVICTVYAVLTRKIPEAFNESKHIGFTMYTTCVIWLAFVPLYFGTANHVPLRITSMSVTISLSASVTIACLFSPKLYIILIRPERNVRQSMMPPRYGNMHRTAGTGPSSMMAAAVVTAATCAQEEKIQKHITPTNTENSLKTKKLCEMATQTISSIITSLDINAYNQIPYADQYVPNNATTATTTPTDKGSGNGNSNSNSNTNGSSNSNSNSNGDGDVATEQVVANNNKINQRQHGQPAVAFAITSTNNHISGPAATITTTAATGETTTEALVATITTPLATVDGSETMSVAPNNGHGNNGSQRPPVLQTNL*LNAQQLQQQLLKEQQRQQAELQTQHPQQQHGTQSSSSDTASTGSGSAAKKRAAIPV

Target ORF information:

RefSeq Version	NM_001273877.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mangetout (mtt), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273877.1

ORF Insert Sequence:

ATGACAACTG CCAATATTGT GGCAGCGCCA GCAGCACCGA CCATATCGCC GGCAATAACG 
AGCACGGCGA CCAGGATTGG TGGCAAGGCG GTGCCCACGC CTCGGTTACT CGGATCCGGT 
GCGGCGAATG TGGCCCAGAT ACTGGCCAAG TCTCTGGACA GTCCGTCCCC ATCATCGTCA 
TCCGGCTGGC CGGTTAAGCA TGCCGCTGTG CTCGAGGGCG ATGTTATATT GGGCGGCCTG 
ATGATGGTCC ACTCGCGCGA GGACAGCATC ACTTGCGGAC CCATAATGCC GCAGGGCGGC 
ATCCAGGCCT TGGAGGCGAT GCTATACACC TTGGACCAGG TGAACAAGCA ACAGCTGCTG 
CCGAACGTCA CTCTGGGCGC CCACCTGCTC GACGATTGCG ACAAGGACAC CTACGGCCTG 
GAAATGGCAG TCGACTTTAT TAAAGCGCAT ATACAAATAA ATTCAGAATA TATCACGGTA 
AAAACCGAAG CCAAGGCCAA AATGAAAACA TTCTGTGACG AAAGCAAATG GTCCGTGCAA 
ATTTGTTTGC TCCTGCTCCT GGTCGTTCTG TCCACTTGTC ACATCCTGCA GACCGAAAGC 
CAGGTGGTCG GCGACATCGA ACAGGCGGAC GACGGGGTGG CGTCCAGTCA TCGTCGCAGT 
CGTAGTCACA GTGCCAGCCA TCAAGCTCAC GTTGGTAATG GCAATAATCC TGGCTGCAAG 
CACCTCCATC ATCGTCAGCA TCGCGGCAAA AGTCACGCCC GGAAGCACAG ACAGAGCCAT 
TACGAGATGC TCGAGCTGGA GCAGCGGCAG CAGCGGATGG GCAAGCACTG GCGTCAGTGG 
ATGAGCCACC TGCAGTCGCC GGATTATGCC AACGATGACC CATTGCCAGA TGGCAGGCGC 
TCCTCGCCGG CTAATGCAGA CCTTACCTAC GACCACTTCG AGCTGGAGGA CTACCAGCGA 
TATGCTAATA TTGACCTAGC ATCGCCGCGC TCCGGTCGCC ATCGCGGTCG CCATCTGCCC 
CTGAACAACA TGAACCTAAT GCTGCCGCTC AGCTCGGCGG ATGGTAGTGG CAAGTACCTG 
AGATCCCTGC CGGATTCGGT GAGCTTCCAA AACGATCGAT TGTACGCCAC CCAGCCGCAA 
AGGCACTGGC CCGTCAAGCG GGAGGCTATC GTGGAGGGCG ACGTCATTCT GGGCGGTCTG 
ATGATGGTCC ACTCGCGCGA GGACAGCATC ACCTGCGGTC CCATCATGCC GCAGGGTGGC 
ATCCAGGCCC TGGAGGCCAT GCTCTTCACT ATCGACAGGA TAAACCGGGA GCAGCTCCTG 
CCGAACATTA CCCTGGGTGC CCATATCCTC GACGACTGCG ATAAGGACTC GTATGGCCTT 
GAAATGGCCG TGGATTTTAT CAAAGGGTCC ATCAGCAACA TAGATGACGC GGAGTATCAC 
TGCAATAAGA CGCAGGTGCG CAAGGTCATC TCGGGGGTGG TGGGTGCCGC CTCCTCGGTC 
ACATCCATAC AAGTTGCCAA TTTATTGCGG TTATTCCGAA TTCCCCAGGT GTCGTACTTC 
TCCACCAGCC CCGAGCTGAG CAACAAACAG CGATTCGAGT ACTTCTCCCG CACTATTCCC 
TCGGATCACT ACCAGGTGAA GGCCATGGTG GAGATAGTGA AGCGGATGGG ATGGAGCTAC 
GTGTCGATTA TCTACGAGGA GAGCAATTAC GGAATCAAGG CTTTCGAGGA ACTGGAGGAG 
CTCCTCGCAC GCCATAACAT TTGTATTGCC ATCAAGGAGA AGCTGGTGAA GGATTCGGGA 
GTGGCCGAGG ACATCGCCTA CGACAATATA GTGCAGAAGC TGCTGACGAA GCCGCGGGCT 
CGAGGGGCCA TTATCTTCGG TTCGGATCAA GAGGTGCGCC AAGTGATGCG GGCGGTGCGC 
CGGGCGAATG CAACTGGTTC CTTCTCCTGG ATCGGATCCG ATGGCTGGAG TGCCCGGAAT 
TTGGTGTCCG ACGACTACGA GCCGGAGGTG GAAGGCACGC TGTCTGTGCA GCCGCAGGCG 
AATCCGGTGC GCGGGTTCGA GGAGTACTTC CTCAGTCTGA CGGTGGAGAA CAATCAACGC 
AATCCCTGGT TTGTGGAATT CTGGGAGGAT CACTTTCAGT GCCGATATCC CGGCAGTACG 
AGCACTCCGT ACAATAACTA CACCAAACAG TGCACCACAA AGGAGCGACT TTCACGCCAG 
AACACTGACT TCGAGGACCA GTTGCAGTTT GTCAGCGATG CGGTGATGGC ATTTGCATAC 
GCCCTAAGGG ATATGCACCG CGACCTGTGC GGCGGAGGTC CTTCGCTCTG CGAGGCCATG 
AAGCCAACGA AGGGTGCAGA CTTGCTGAAA TATTTGCGAA AAGTGGAGTT TGAGGGCCTC 
AGCGGCGACG AGTTCCGCTT CGACGGGAAC GGCGACGGTC CCGCCCGGTA CAACATCATC 
CACTTCAAGC AGTCCCAGGC GGGACAGTAC CACTGGGTCA AGGTGGGCGA GTACACCGAG 
GGCGAGCTGC GGCTTAATAT GACGGAGGTG AAGTTTAAGC GGCTGAGTCC CAAGCCGCCG 
GAGTCTGTCT GCAGCCTGCC ATGTCTTGTG GGTCAGGCCA AGAAGTACGT GGAAGGCGAG 
AGCTGCTGTT GGCACTGCTT CAATTGCACA ACCTATCAAA TCCGCCACCC GGATGACGAG 
ACCCATTGCA AGCTGTGCAA GCTGGGCACT CTTCCCGACG CCCACAAGCA GTACTGCCGC 
CCCATCCCGG AGATATATTT GCGTCCGGAG TCCGCTTGGG CCATCGGTGC TATGGCGTTC 
AGTGCGACCG GGATCTTAGT AACGCTCTTC GTAATGGGCG TATTTGTCAG GCACAACGAC 
ACGCCCATAG TGCGTGCCTC TGGTCGGGAG CTGAGCTACA TCCTGCTGGC GGGGATTTTC 
ATGTGCTACG GTGTCACCTT TGCCCTGGTC CTGAAGCCGA CCAACATAGT GTGTGCCATT 
CAGCGGTTTG GTGTCGGCTT CTGCTTCACG GTCGTTTATG CAGCACTGTT GACCAAAACG 
AATCGCATTG CACGGATCTT CAAAGCGGGC AAACAATCCG CCAAGAGGCC CTCCTTCATT 
AGTCCCAAGT CCCAGCTGGT AATCTGTGCG TGCCTGGTTA GTGTGCAGAT ACTCATCAAC 
GGCGTTTGGA TGGTAATTGC GCCATCACAT GCCATGCACC ATTATCCCAC ACGCGAGGAT 
AATCTGCTCG TCTGCGACTC GTACATCGAC GCCTCCTACA TGATTGCGTT CTCCTACCCA 
ATCTTCCTGA TTGTTATTTG CACGGTCTAC GCGGTGCTCA CGCGAAAGAT TCCGGAGGCT 
TTCAACGAAT CGAAGCATAT TGGATTCACC ATGTACACGA CTTGTGTGAT TTGGCTGGCT 
TTTGTACCGC TCTACTTCGG AACCGCCAAC CACGTTCCGC TGCGCATCAC CAGCATGTCG 
GTGACCATCA GCTTATCCGC CAGCGTGACC ATCGCCTGTC TCTTTTCGCC CAAGCTGTAC 
ATAATACTCA TACGCCCCGA GCGCAACGTG CGCCAGAGTA TGATGCCGCC TCGCTACGGC 
AACATGCACC GCACCGCTGG CACAGGACCC TCGTCCATGA TGGCCGCCGC CGTGGTGACC 
GCCGCCACCT GTGCCCAGGA GGAGAAGATC CAGAAGCATA TCACTCCCAC AAACACAGAA 
AACTCGCTAA AGACGAAGAA ATTGTGCGAA ATGGCCACTC AGACAATTTC CAGTATAATC 
ACGTCGCTGG ACATCAATGC GTACAATCAA ATACCATATG CCGATCAATA TGTGCCAAAC 
AATGCCACAA CGGCCACGAC CACGCCCACA GATAAGGGCA GTGGCAACGG CAACAGCAAC 
AGCAATAGCA ATACGAATGG TAGTAGCAAT AGCAACAGCA ACAGCAATGG CGATGGCGAT 
GTGGCAACAG AGCAAGTCGT GGCCAACAAT AATAAAATCA ATCAGCGACA ACATGGCCAG 
CCGGCCGTGG CATTTGCCAT CACATCGACT AACAATCACA TAAGCGGCCC AGCAGCAACC 
ATAACAACAA CAGCGGCAAC TGGCGAAACA ACAACTGAGG CGCTGGTGGC TACAATAACA 
ACTCCATTGG CAACGGTGGA TGGCAGCGAA ACCATGTCCG TGGCTCCCAA CAATGGGCAT 
GGAAACAACG GGAGCCAACG TCCGCCGGTC CTGCAAACAA ACTTATAGCT AAACGCGCAA 
CAATTGCAGC AACAGCTGCT GAAGGAGCAG CAGCGACAAC AAGCCGAGCT GCAAACGCAA 
CATCCGCAGC AGCAACATGG AACACAAAGC TCTTCCAGCG ACACCGCCAG CACGGGCAGC 
GGCAGTGCGG CGAAGAAGCG GGCCGCAATT CCCGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mtt ( NM_001144147.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137619.1 Drosophila melanogaster mangetout (mtt), transcript variant D, mRNA.

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CloneID	OFa12773
Clone ID Related Accession (Same CDS sequence)	NM_001144147.2
Accession Version	NM_001144147.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4248bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mangetout, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001144147.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAACTG CCAATATTGT GGCAGCGCCA GCAGCACCGA CCATATCGCC GGCAATAACG 
AGCACGGCGA CCAGGATTGG TGGCAAGGCG GTGCCCACGC CTCGGTTACT CGGATCCGGT 
GCGGCGAATG TGGCCCAGAT ACTGGCCAAG TCTCTGGACA GTCCGTCCCC ATCATCGTCA 
TCCGGCTGGC CGGTTAAGCA TGCCGCTGTG CTCGAGGGCG ATGTTATATT GGGCGGCCTG 
ATGATGGTCC ACTCGCGCGA GGACAGCATC ACTTGCGGAC CCATAATGCC GCAGGGCGGC 
ATCCAGGCCT TGGAGGCGAT GCTATACACC TTGGACCAGG TGAACAAGCA ACAGCTGCTG 
CCGAACGTCA CTCTGGGCGC CCACCTGCTC GACGATTGCG ACAAGGACAC CTACGGCCTG 
GAAATGGCAG TCGACTTTAT TAAAGCGCAT ATACAAATAA ATTCAGAATA TATCACGGTA 
AAAACCGAAG CCAAGGCCAA AATGAAAACA TTCTGTGACG AAAGCAAATG GTCCGTGCAA 
ATTTGTTTGC TCCTGCTCCT GGTCGTTCTG TCCACTTGTC ACATCCTGCA GACCGAAAGC 
CAGGTGGTCG GCGACATCGA ACAGGCGGAC GACGGGGTGG CGTCCAGTCA TCGTCGCAGT 
CGTAGTCACA GTGCCAGCCA TCAAGCTCAC GTTGGTAATG GCAATAATCC TGGCTGCAAG 
CACCTCCATC ATCGTCAGCA TCGCGGCAAA AGTCACGCCC GGAAGCACAG ACAGAGCCAT 
TACGAGATGC TCGAGCTGGA GCAGCGGCAG CAGCGGATGG GCAAGCACTG GCGTCAGTGG 
ATGAGCCACC TGCAGTCGCC GGATTATGCC AACGATGACC CATTGCCAGA TGGCAGGCGC 
TCCTCGCCGG CTAATGCAGA CCTTACCTAC GACCACTTCG AGCTGGAGGA CTACCAGCGA 
TATGCTAATA TTGACCTAGC ATCGCCGCGC TCCGGTCGCC ATCGCGGTCG CCATCTGCCC 
CTGAACAACA TGAACCTAAT GCTGCCGCTC AGCTCGGCGG ATGGTAGTGG CAAGTACCTG 
AGATCCCTGC CGGATTCGGT GAGCTTCCAA AACGATCGAT TGTACGCCAC CCAGCCGCAA 
AGGCACTGGC CCGTCAAGCG GGAGGCTATC GTGGAGGGCG ACGTCATTCT GGGCGGTCTG 
ATGATGGTCC ACTCGCGCGA GGACAGCATC ACCTGCGGTC CCATCATGCC GCAGGGTGGC 
ATCCAGGCCC TGGAGGCCAT GCTCTTCACT ATCGACAGGA TAAACCGGGA GCAGCTCCTG 
CCGAACATTA CCCTGGGTGC CCATATCCTC GACGACTGCG ATAAGGACTC GTATGGCCTT 
GAAATGGCCG TGGATTTTAT CAAAGGGTCC ATCAGCAACA TAGATGACGC GGAGTATCAC 
TGCAATAAGA CGCAGGTGCG CAAGGTCATC TCGGGGGTGG TGGGTGCCGC CTCCTCGGTC 
ACATCCATAC AAGTTGCCAA TTTATTGCGG TTATTCCGAA TTCCCCAGGT GTCGTACTTC 
TCCACCAGCC CCGAGCTGAG CAACAAACAG CGATTCGAGT ACTTCTCCCG CACTATTCCC 
TCGGATCACT ACCAGGTGAA GGCCATGGTG GAGATAGTGA AGCGGATGGG ATGGAGCTAC 
GTGTCGATTA TCTACGAGGA GAGCAATTAC GGAATCAAGG CTTTCGAGGA ACTGGAGGAG 
CTCCTCGCAC GCCATAACAT TTGTATTGCC ATCAAGGAGA AGCTGGTGAA GGATTCGGGA 
GTGGCCGAGG ACATCGCCTA CGACAATATA GTGCAGAAGC TGCTGACGAA GCCGCGGGCT 
CGAGGGGCCA TTATCTTCGG TTCGGATCAA GAGGTGCGCC AAGTGATGCG GGCGGTGCGC 
CGGGCGAATG CAACTGGTTC CTTCTCCTGG ATCGGATCCG ATGGCTGGAG TGCCCGGAAT 
TTGGTGTCCG ACGACTACGA GCCGGAGGTG GAAGGCACGC TGTCTGTGCA GCCGCAGGCG 
AATCCGGTGC GCGGGTTCGA GGAGTACTTC CTCAGTCTGA CGGTGGAGAA CAATCAACGC 
AATCCCTGGT TTGTGGAATT CTGGGAGGAT CACTTTCAGT GCCGATATCC CGGCAGTACG 
AGCACTCCGT ACAATAACTA CACCAAACAG TGCACCACAA AGGAGCGACT TTCACGCCAG 
AACACTGACT TCGAGGACCA GTTGCAGTTT GTCAGCGATG CGGTGATGGC ATTTGCATAC 
GCCCTAAGGG ATATGCACCG CGACCTGTGC GGCGGAGGTC CTTCGCTCTG CGAGGCCATG 
AAGCCAACGA AGGGTGCAGA CTTGCTGAAA TATTTGCGAA AAGTGGAGTT TGAGGGCCTC 
AGCGGCGACG AGTTCCGCTT CGACGGGAAC GGCGACGGTC CCGCCCGGTA CAACATCATC 
CACTTCAAGC AGTCCCAGGC GGGACAGTAC CACTGGGTCA AGGTGGGCGA GTACACCGAG 
GGCGAGCTGC GGCTTAATAT GACGGAGGTG AAGTTTAAGC GGCTGAGTCC CAAGCCGCCG 
GAGTCTGTCT GCAGCCTGCC ATGTCTTGTG GGTCAGGCCA AGAAGTACGT GGAAGGCGAG 
AGCTGCTGTT GGCACTGCTT CAATTGCACA ACCTATCAAA TCCGCCACCC GGATGACGAG 
ACCCATTGCA AGCTGTGCAA GCTGGGCACT CTTCCCGACG CCCACAAGCA GTACTGCCGC 
CCCATCCCGG AGATATATTT GCGTCCGGAG TCCGCTTGGG CCATCGGTGC TATGGCGTTC 
AGTGCGACCG GGATCTTAGT AACGCTCTTC GTAATGGGCG TATTTGTCAG GCACAACGAC 
ACGCCCATAG TGCGTGCCTC TGGTCGGGAG CTGAGCTACA TCCTGCTGGC GGGGATTTTC 
ATGTGCTACG GTGTCACCTT TGCCCTGGTC CTGAAGCCGA CCAACATAGT GTGTGCCATT 
CAGCGGTTTG GTGTCGGCTT CTGCTTCACG GTCGTTTATG CAGCACTGTT GACCAAAACG 
AATCGCATTG CACGGATCTT CAAAGCGGGC AAACAATCCG CCAAGAGGCC CTCCTTCATT 
AGTCCCAAGT CCCAGCTGGT AATCTGTGCG TGCCTGGTTA GTGTGCAGAT ACTCATCAAC 
GGCGTTTGGA TGGTAATTGC GCCATCACAT GCCATGCACC ATTATCCCAC ACGCGAGGAT 
AATCTGCTCG TCTGCGACTC GTACATCGAC GCCTCCTACA TGATTGCGTT CTCCTACCCA 
ATCTTCCTGA TTGTTATTTG CACGGTCTAC GCGGTGCTCA CGCGAAAGAT TCCGGAGGCT 
TTCAACGAAT CGAAGCATAT TGGATTCACC ATGTACACGA CTTGTGTGAT TTGGCTGGCT 
TTTGTACCGC TCTACTTCGG AACCGCCAAC CACGTTCCGC TGCGCATCAC CAGCATGTCG 
GTGACCATCA GCTTATCCGC CAGCGTGACC ATCGCCTGTC TCTTTTCGCC CAAGCTGTAC 
ATAATACTCA TACGCCCCGA GCGCAACGTG CGCCAGAGTA TGATGCCGCC TCGCTACGGC 
AACATGCACC GCACCGCTGG CACAGGACCC TCGTCCATGA TGGCCGCCGC CGTGGTGACC 
GCCGCCACCT GTGCCCAGGA GGAGAAGATC CAGAAGCATA TCACTCCCAC AAACACAGAA 
AACTCGCTAA AGACGAAGAA ATTGTGCGAA ATGGCCACTC AGACAATTTC CAGTATAATC 
ACGTCGCTGG ACATCAATGC GTACAATCAA ATACCATATG CCGATCAATA TGTGCCAAAC 
AATGCCACAA CGGCCACGAC CACGCCCACA GATAAGGGCA GTGGCAACGG CAACAGCAAC 
AGCAATAGCA ATACGAATGG TAGTAGCAAT AGCAACAGCA ACAGCAATGG CGATGGCGAT 
GTGGCAACAG AGCAAGTCGT GGCCAACAAT AATAAAATCA ATCAGCGACA ACATGGCCAG 
CCGGCCGTGG CATTTGCCAT CACATCGACT AACAATCACA TAAGCGGCCC AGCAGCAACC 
ATAACAACAA CAGCGGCAAC TGGCGAAACA ACAACTGAGG CGCTGGTGGC TACAATAACA 
ACTCCATTGG CAACGGTGGA TGGCAGCGAA ACCATGTCCG TGGCTCCCAA CAATGGGCAT 
GGAAACAACG GGAGCCAACG TCCGCCGGTC CTGCAAACAA ACTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137619.1
CDS	1622..5869
Translation	MTTANIVAAPAAPTISPAITSTATRIGGKAVPTPRLLGSGAANVAQILAKSLDSPSPSSSSGWPVKHAAVLEGDVILGGLMMVHSREDSITCGPIMPQGGIQALEAMLYTLDQVNKQQLLPNVTLGAHLLDDCDKDTYGLEMAVDFIKAHIQINSEYITVKTEAKAKMKTFCDESKWSVQICLLLLLVVLSTCHILQTESQVVGDIEQADDGVASSHRRSRSHSASHQAHVGNGNNPGCKHLHHRQHRGKSHARKHRQSHYEMLELEQRQQRMGKHWRQWMSHLQSPDYANDDPLPDGRRSSPANADLTYDHFELEDYQRYANIDLASPRSGRHRGRHLPLNNMNLMLPLSSADGSGKYLRSLPDSVSFQNDRLYATQPQRHWPVKREAIVEGDVILGGLMMVHSREDSITCGPIMPQGGIQALEAMLFTIDRINREQLLPNITLGAHILDDCDKDSYGLEMAVDFIKGSISNIDDAEYHCNKTQVRKVISGVVGAASSVTSIQVANLLRLFRIPQVSYFSTSPELSNKQRFEYFSRTIPSDHYQVKAMVEIVKRMGWSYVSIIYEESNYGIKAFEELEELLARHNICIAIKEKLVKDSGVAEDIAYDNIVQKLLTKPRARGAIIFGSDQEVRQVMRAVRRANATGSFSWIGSDGWSARNLVSDDYEPEVEGTLSVQPQANPVRGFEEYFLSLTVENNQRNPWFVEFWEDHFQCRYPGSTSTPYNNYTKQCTTKERLSRQNTDFEDQLQFVSDAVMAFAYALRDMHRDLCGGGPSLCEAMKPTKGADLLKYLRKVEFEGLSGDEFRFDGNGDGPARYNIIHFKQSQAGQYHWVKVGEYTEGELRLNMTEVKFKRLSPKPPESVCSLPCLVGQAKKYVEGESCCWHCFNCTTYQIRHPDDETHCKLCKLGTLPDAHKQYCRPIPEIYLRPESAWAIGAMAFSATGILVTLFVMGVFVRHNDTPIVRASGRELSYILLAGIFMCYGVTFALVLKPTNIVCAIQRFGVGFCFTVVYAALLTKTNRIARIFKAGKQSAKRPSFISPKSQLVICACLVSVQILINGVWMVIAPSHAMHHYPTREDNLLVCDSYIDASYMIAFSYPIFLIVICTVYAVLTRKIPEAFNESKHIGFTMYTTCVIWLAFVPLYFGTANHVPLRITSMSVTISLSASVTIACLFSPKLYIILIRPERNVRQSMMPPRYGNMHRTAGTGPSSMMAAAVVTAATCAQEEKIQKHITPTNTENSLKTKKLCEMATQTISSIITSLDINAYNQIPYADQYVPNNATTATTTPTDKGSGNGNSNSNSNTNGSSNSNSNSNGDGDVATEQVVANNNKINQRQHGQPAVAFAITSTNNHISGPAATITTTAATGETTTEALVATITTPLATVDGSETMSVAPNNGHGNNGSQRPPVLQTNL

Target ORF information:

RefSeq Version	NM_001144147.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mangetout (mtt), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144147.2

ORF Insert Sequence:

ATGACAACTG CCAATATTGT GGCAGCGCCA GCAGCACCGA CCATATCGCC GGCAATAACG 
AGCACGGCGA CCAGGATTGG TGGCAAGGCG GTGCCCACGC CTCGGTTACT CGGATCCGGT 
GCGGCGAATG TGGCCCAGAT ACTGGCCAAG TCTCTGGACA GTCCGTCCCC ATCATCGTCA 
TCCGGCTGGC CGGTTAAGCA TGCCGCTGTG CTCGAGGGCG ATGTTATATT GGGCGGCCTG 
ATGATGGTCC ACTCGCGCGA GGACAGCATC ACTTGCGGAC CCATAATGCC GCAGGGCGGC 
ATCCAGGCCT TGGAGGCGAT GCTATACACC TTGGACCAGG TGAACAAGCA ACAGCTGCTG 
CCGAACGTCA CTCTGGGCGC CCACCTGCTC GACGATTGCG ACAAGGACAC CTACGGCCTG 
GAAATGGCAG TCGACTTTAT TAAAGCGCAT ATACAAATAA ATTCAGAATA TATCACGGTA 
AAAACCGAAG CCAAGGCCAA AATGAAAACA TTCTGTGACG AAAGCAAATG GTCCGTGCAA 
ATTTGTTTGC TCCTGCTCCT GGTCGTTCTG TCCACTTGTC ACATCCTGCA GACCGAAAGC 
CAGGTGGTCG GCGACATCGA ACAGGCGGAC GACGGGGTGG CGTCCAGTCA TCGTCGCAGT 
CGTAGTCACA GTGCCAGCCA TCAAGCTCAC GTTGGTAATG GCAATAATCC TGGCTGCAAG 
CACCTCCATC ATCGTCAGCA TCGCGGCAAA AGTCACGCCC GGAAGCACAG ACAGAGCCAT 
TACGAGATGC TCGAGCTGGA GCAGCGGCAG CAGCGGATGG GCAAGCACTG GCGTCAGTGG 
ATGAGCCACC TGCAGTCGCC GGATTATGCC AACGATGACC CATTGCCAGA TGGCAGGCGC 
TCCTCGCCGG CTAATGCAGA CCTTACCTAC GACCACTTCG AGCTGGAGGA CTACCAGCGA 
TATGCTAATA TTGACCTAGC ATCGCCGCGC TCCGGTCGCC ATCGCGGTCG CCATCTGCCC 
CTGAACAACA TGAACCTAAT GCTGCCGCTC AGCTCGGCGG ATGGTAGTGG CAAGTACCTG 
AGATCCCTGC CGGATTCGGT GAGCTTCCAA AACGATCGAT TGTACGCCAC CCAGCCGCAA 
AGGCACTGGC CCGTCAAGCG GGAGGCTATC GTGGAGGGCG ACGTCATTCT GGGCGGTCTG 
ATGATGGTCC ACTCGCGCGA GGACAGCATC ACCTGCGGTC CCATCATGCC GCAGGGTGGC 
ATCCAGGCCC TGGAGGCCAT GCTCTTCACT ATCGACAGGA TAAACCGGGA GCAGCTCCTG 
CCGAACATTA CCCTGGGTGC CCATATCCTC GACGACTGCG ATAAGGACTC GTATGGCCTT 
GAAATGGCCG TGGATTTTAT CAAAGGGTCC ATCAGCAACA TAGATGACGC GGAGTATCAC 
TGCAATAAGA CGCAGGTGCG CAAGGTCATC TCGGGGGTGG TGGGTGCCGC CTCCTCGGTC 
ACATCCATAC AAGTTGCCAA TTTATTGCGG TTATTCCGAA TTCCCCAGGT GTCGTACTTC 
TCCACCAGCC CCGAGCTGAG CAACAAACAG CGATTCGAGT ACTTCTCCCG CACTATTCCC 
TCGGATCACT ACCAGGTGAA GGCCATGGTG GAGATAGTGA AGCGGATGGG ATGGAGCTAC 
GTGTCGATTA TCTACGAGGA GAGCAATTAC GGAATCAAGG CTTTCGAGGA ACTGGAGGAG 
CTCCTCGCAC GCCATAACAT TTGTATTGCC ATCAAGGAGA AGCTGGTGAA GGATTCGGGA 
GTGGCCGAGG ACATCGCCTA CGACAATATA GTGCAGAAGC TGCTGACGAA GCCGCGGGCT 
CGAGGGGCCA TTATCTTCGG TTCGGATCAA GAGGTGCGCC AAGTGATGCG GGCGGTGCGC 
CGGGCGAATG CAACTGGTTC CTTCTCCTGG ATCGGATCCG ATGGCTGGAG TGCCCGGAAT 
TTGGTGTCCG ACGACTACGA GCCGGAGGTG GAAGGCACGC TGTCTGTGCA GCCGCAGGCG 
AATCCGGTGC GCGGGTTCGA GGAGTACTTC CTCAGTCTGA CGGTGGAGAA CAATCAACGC 
AATCCCTGGT TTGTGGAATT CTGGGAGGAT CACTTTCAGT GCCGATATCC CGGCAGTACG 
AGCACTCCGT ACAATAACTA CACCAAACAG TGCACCACAA AGGAGCGACT TTCACGCCAG 
AACACTGACT TCGAGGACCA GTTGCAGTTT GTCAGCGATG CGGTGATGGC ATTTGCATAC 
GCCCTAAGGG ATATGCACCG CGACCTGTGC GGCGGAGGTC CTTCGCTCTG CGAGGCCATG 
AAGCCAACGA AGGGTGCAGA CTTGCTGAAA TATTTGCGAA AAGTGGAGTT TGAGGGCCTC 
AGCGGCGACG AGTTCCGCTT CGACGGGAAC GGCGACGGTC CCGCCCGGTA CAACATCATC 
CACTTCAAGC AGTCCCAGGC GGGACAGTAC CACTGGGTCA AGGTGGGCGA GTACACCGAG 
GGCGAGCTGC GGCTTAATAT GACGGAGGTG AAGTTTAAGC GGCTGAGTCC CAAGCCGCCG 
GAGTCTGTCT GCAGCCTGCC ATGTCTTGTG GGTCAGGCCA AGAAGTACGT GGAAGGCGAG 
AGCTGCTGTT GGCACTGCTT CAATTGCACA ACCTATCAAA TCCGCCACCC GGATGACGAG 
ACCCATTGCA AGCTGTGCAA GCTGGGCACT CTTCCCGACG CCCACAAGCA GTACTGCCGC 
CCCATCCCGG AGATATATTT GCGTCCGGAG TCCGCTTGGG CCATCGGTGC TATGGCGTTC 
AGTGCGACCG GGATCTTAGT AACGCTCTTC GTAATGGGCG TATTTGTCAG GCACAACGAC 
ACGCCCATAG TGCGTGCCTC TGGTCGGGAG CTGAGCTACA TCCTGCTGGC GGGGATTTTC 
ATGTGCTACG GTGTCACCTT TGCCCTGGTC CTGAAGCCGA CCAACATAGT GTGTGCCATT 
CAGCGGTTTG GTGTCGGCTT CTGCTTCACG GTCGTTTATG CAGCACTGTT GACCAAAACG 
AATCGCATTG CACGGATCTT CAAAGCGGGC AAACAATCCG CCAAGAGGCC CTCCTTCATT 
AGTCCCAAGT CCCAGCTGGT AATCTGTGCG TGCCTGGTTA GTGTGCAGAT ACTCATCAAC 
GGCGTTTGGA TGGTAATTGC GCCATCACAT GCCATGCACC ATTATCCCAC ACGCGAGGAT 
AATCTGCTCG TCTGCGACTC GTACATCGAC GCCTCCTACA TGATTGCGTT CTCCTACCCA 
ATCTTCCTGA TTGTTATTTG CACGGTCTAC GCGGTGCTCA CGCGAAAGAT TCCGGAGGCT 
TTCAACGAAT CGAAGCATAT TGGATTCACC ATGTACACGA CTTGTGTGAT TTGGCTGGCT 
TTTGTACCGC TCTACTTCGG AACCGCCAAC CACGTTCCGC TGCGCATCAC CAGCATGTCG 
GTGACCATCA GCTTATCCGC CAGCGTGACC ATCGCCTGTC TCTTTTCGCC CAAGCTGTAC 
ATAATACTCA TACGCCCCGA GCGCAACGTG CGCCAGAGTA TGATGCCGCC TCGCTACGGC 
AACATGCACC GCACCGCTGG CACAGGACCC TCGTCCATGA TGGCCGCCGC CGTGGTGACC 
GCCGCCACCT GTGCCCAGGA GGAGAAGATC CAGAAGCATA TCACTCCCAC AAACACAGAA 
AACTCGCTAA AGACGAAGAA ATTGTGCGAA ATGGCCACTC AGACAATTTC CAGTATAATC 
ACGTCGCTGG ACATCAATGC GTACAATCAA ATACCATATG CCGATCAATA TGTGCCAAAC 
AATGCCACAA CGGCCACGAC CACGCCCACA GATAAGGGCA GTGGCAACGG CAACAGCAAC 
AGCAATAGCA ATACGAATGG TAGTAGCAAT AGCAACAGCA ACAGCAATGG CGATGGCGAT 
GTGGCAACAG AGCAAGTCGT GGCCAACAAT AATAAAATCA ATCAGCGACA ACATGGCCAG 
CCGGCCGTGG CATTTGCCAT CACATCGACT AACAATCACA TAAGCGGCCC AGCAGCAACC 
ATAACAACAA CAGCGGCAAC TGGCGAAACA ACAACTGAGG CGCTGGTGGC TACAATAACA 
ACTCCATTGG CAACGGTGGA TGGCAGCGAA ACCATGTCCG TGGCTCCCAA CAATGGGCAT 
GGAAACAACG GGAGCCAACG TCCGCCGGTC CTGCAAACAA ACTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mtt ( NM_136542.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610386.2 Drosophila melanogaster mangetout (mtt), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa12772
Clone ID Related Accession (Same CDS sequence)	NM_136542.3
Accession Version	NM_136542.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mangetout, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_136542.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAACTG CCAATATTGT GGCAGCGCCA GCAGCACCGA CCATATCGCC GGCAATAACG 
AGCACGGCGA CCAGGATTGG TGGCAAGGCG GTGCCCACGC CTCGGTTACT CGGATCCGGT 
GCGGCGAATG TGGCCCAGAT ACTGGCCAAG TCTCTGGACA GTCCGTCCCC ATCATCGTCA 
TCCGGCTGGC CGGTTAAGCA TGCCGCTGTG CTCGAGGGCG ATGTTATATT GGGCGGCCTG 
ATGATGGTCC ACTCGCGCGA GGACAGCATC ACTTGCGGAC CCATAATGCC GCAGGGCGGC 
ATCCAGGCCT TGGAGGCGAT GCTATACACC TTGGACCAGG TGAACAAGCA ACAGCTGCTG 
CCGAACGTCA CTCTGGGCGC CCACCTGCTC GACGATTGCG ACAAGGACAC CTACGGCCTG 
GAAATGGCAG TCGACTTTAT TAAAGGGTCC ATCAGCAACA TAGATGACGC GGAGTATCAC 
TGCAATAAGA CGCAGGTGCG CAAGGTCATC TCGGGGGTGG TGGGTGCCGC CTCCTCGGTC 
ACATCCATAC AAGTTGCCAA TTTATTGCGG TTATTCCGAA TTCCCCAGGT GTCGTACTTC 
TCCACCAGCC CCGAGCTGAG CAACAAACAG CGATTCGAGT ACTTCTCCCG CACTATTCCC 
TCGGATCACT ACCAGGTGAA GGCCATGGTG GAGATAGTGA AGCGGATGGG ATGGAGCTAC 
GTGTCGATTA TCTACGAGGA GAGCAATTAC GGAATCAAGG CTTTCGAGGA ACTGGAGGAG 
CTCCTCGCAC GCCATAACAT TTGTATTGCC ATCAAGGAGA AGCTGGTGAA GGATTCGGGA 
GTGGCCGAGG ACATCGCCTA CGACAATATA GTGCAGAAGC TGCTGACGAA GCCGCGGGCT 
CGAGGGGCCA TTATCTTCGG TTCGGATCAA GAGGTGCGCC AAGTGATGCG GGCGGTGCGC 
CGGGCGAATG CAACTGGTTC CTTCTCCTGG ATCGGATCCG ATGGCTGGAG TGCCCGGAAT 
TTGGTGTCCG ACGACTACGA GCCGGAGGTG GAAGGCACGC TGTCTGTGCA GCCGCAGGCG 
AATCCGGTGC GCGGGTTCGA GGAGTACTTC CTCAGTCTGA CGGTGGAGAA CAATCAACGC 
AATCCCTGGT TTGTGGAATT CTGGGAGGAT CACTTTCAGT GCCGATATCC CGGCAGTACG 
AGCACTCCGT ACAATAACTA CACCAAACAG TGCACCACAA AGGAGCGACT TTCACGCCAG 
AACACTGACT TCGAGGACCA GTTGCAGTTT GTCAGCGATG CGGTGATGGC ATTTGCATAC 
GCCCTAAGGG ATATGCACCG CGACCTGTGC GGCGGAGGTC CTTCGCTCTG CGAGGCCATG 
AAGCCAACGA AGGGTGCAGA CTTGCTGAAA TATTTGCGAA AAGTGGAGTT TGAGGGCCTC 
AGCGGCGACG AGTTCCGCTT CGACGGGAAC GGCGACGGTC CCGCCCGGTA CAACATCATC 
CACTTCAAGC AGTCCCAGGC GGGACAGTAC CACTGGGTCA AGGTGGGCGA GTACACCGAG 
GGCGAGCTGC GGCTTAATAT GACGGAGGTG AAGTTTAAGC GGCTGAGTCC CAAGCCGCCG 
GAGTCTGTCT GCAGCCTGCC ATGTCTTGTG GGTCAGGCCA AGAAGTACGT GGAAGGCGAG 
AGCTGCTGTT GGCACTGCTT CAATTGCACA ACCTATCAAA TCCGCCACCC GGATGACGAG 
ACCCATTGCA AGCTGTGCAA GCTGGGCACT CTTCCCGACG CCCACAAGCA GTACTGCCGC 
CCCATCCCGG AGATATATTT GCGTCCGGAG TCCGCTTGGG CCATCGGTGC TATGGCGTTC 
AGTGCGACCG GGATCTTAGT AACGCTCTTC GTAATGGGCG TATTTGTCAG GCACAACGAC 
ACGCCCATAG TGCGTGCCTC TGGTCGGGAG CTGAGCTACA TCCTGCTGGC GGGGATTTTC 
ATGTGCTACG GTGTCACCTT TGCCCTGGTC CTGAAGCCGA CCAACATAGT GTGTGCCATT 
CAGCGGTTTG GTGTCGGCTT CTGCTTCACG GTCGTTTATG CAGCACTGTT GACCAAAACG 
AATCGCATTG CACGGATCTT CAAAGCGGGC AAACAATCCG CCAAGAGGCC CTCCTTCATT 
AGTCCCAAGT CCCAGCTGGT AATCTGTGCG TGCCTGGTTA GTGTGCAGAT ACTCATCAAC 
GGCGTTTGGA TGGTAATTGC GCCATCACAT GCCATGCACC ATTATCCCAC ACGCGAGGAT 
AATCTGCTCG TCTGCGACTC GTACATCGAC GCCTCCTACA TGATTGCGTT CTCCTACCCA 
ATCTTCCTGA TTGTTATTTG CACGGTCTAC GCGGTGCTCA CGCGAAAGAT TCCGGAGGCT 
TTCAACGAAT CGAAGCATAT TGGATTCACC ATGTACACGA CTTGTGTGAT TTGGCTGGCT 
TTTGTACCGC TCTACTTCGG AACCGCCAAC CACGTTCCGC TGCGCATCAC CAGCATGTCG 
GTGACCATCA GCTTATCCGC CAGCGTGACC ATCGCCTGTC TCTTTTCGCC CAAGCTGTAC 
ATAATACTCA TACGCCCCGA GCGCAACGTG CGCCAGAGTA TGATGCCGCC TCGCTACGGC 
AACATGCACC GCACCGCTGG CACAGGACCC TCGTCCATGA TGGCCGCCGC CGTGGTGACC 
GCCGCCACCT GTGCCCAGGA GGAGAAGATC CAGAAGCATA TCACTCCCAC AAACACAGAA 
AACTCGCTAA AGACGAAGAA ATTGTGCGAA ATGGCCACTC AGACAATTTC CAGTATAATC 
ACGTCGCTGG ACATCAATGC GTACAATCAA ATACCATATG CCGATCAATA TGTGCCAAAC 
AATGCCACAA CGGCCACGAC CACGCCCACA GATAAGGGCA GTGGCAACGG CAACAGCAAC 
AGCAATAGCA ATACGAATGG TAGTAGCAAT AGCAACAGCA ACAGCAATGG CGATGGCGAT 
GTGGCAACAG AGCAAGTCGT GGCCAACAAT AATAAAATCA ATCAGCGACA ACATGGCCAG 
CCGGCCGTGG CATTTGCCAT CACATCGACT AACAATCACA TAAGCGGCCC AGCAGCAACC 
ATAACAACAA CAGCGGCAAC TGGCGAAACA ACAACTGAGG CGCTGGTGGC TACAATAACA 
ACTCCATTGG CAACGGTGGA TGGCAGCGAA ACCATGTCCG TGGCTCCCAA CAATGGGCAT 
GGAAACAACG GGAGCCAACG TCCGCCGGTC CTGCAAACAA ACTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610386.2
CDS	1622..4909
Translation	MTTANIVAAPAAPTISPAITSTATRIGGKAVPTPRLLGSGAANVAQILAKSLDSPSPSSSSGWPVKHAAVLEGDVILGGLMMVHSREDSITCGPIMPQGGIQALEAMLYTLDQVNKQQLLPNVTLGAHLLDDCDKDTYGLEMAVDFIKGSISNIDDAEYHCNKTQVRKVISGVVGAASSVTSIQVANLLRLFRIPQVSYFSTSPELSNKQRFEYFSRTIPSDHYQVKAMVEIVKRMGWSYVSIIYEESNYGIKAFEELEELLARHNICIAIKEKLVKDSGVAEDIAYDNIVQKLLTKPRARGAIIFGSDQEVRQVMRAVRRANATGSFSWIGSDGWSARNLVSDDYEPEVEGTLSVQPQANPVRGFEEYFLSLTVENNQRNPWFVEFWEDHFQCRYPGSTSTPYNNYTKQCTTKERLSRQNTDFEDQLQFVSDAVMAFAYALRDMHRDLCGGGPSLCEAMKPTKGADLLKYLRKVEFEGLSGDEFRFDGNGDGPARYNIIHFKQSQAGQYHWVKVGEYTEGELRLNMTEVKFKRLSPKPPESVCSLPCLVGQAKKYVEGESCCWHCFNCTTYQIRHPDDETHCKLCKLGTLPDAHKQYCRPIPEIYLRPESAWAIGAMAFSATGILVTLFVMGVFVRHNDTPIVRASGRELSYILLAGIFMCYGVTFALVLKPTNIVCAIQRFGVGFCFTVVYAALLTKTNRIARIFKAGKQSAKRPSFISPKSQLVICACLVSVQILINGVWMVIAPSHAMHHYPTREDNLLVCDSYIDASYMIAFSYPIFLIVICTVYAVLTRKIPEAFNESKHIGFTMYTTCVIWLAFVPLYFGTANHVPLRITSMSVTISLSASVTIACLFSPKLYIILIRPERNVRQSMMPPRYGNMHRTAGTGPSSMMAAAVVTAATCAQEEKIQKHITPTNTENSLKTKKLCEMATQTISSIITSLDINAYNQIPYADQYVPNNATTATTTPTDKGSGNGNSNSNSNTNGSSNSNSNSNGDGDVATEQVVANNNKINQRQHGQPAVAFAITSTNNHISGPAATITTTAATGETTTEALVATITTPLATVDGSETMSVAPNNGHGNNGSQRPPVLQTNL

Target ORF information:

RefSeq Version	NM_136542.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mangetout (mtt), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136542.3

ORF Insert Sequence:

ATGACAACTG CCAATATTGT GGCAGCGCCA GCAGCACCGA CCATATCGCC GGCAATAACG 
AGCACGGCGA CCAGGATTGG TGGCAAGGCG GTGCCCACGC CTCGGTTACT CGGATCCGGT 
GCGGCGAATG TGGCCCAGAT ACTGGCCAAG TCTCTGGACA GTCCGTCCCC ATCATCGTCA 
TCCGGCTGGC CGGTTAAGCA TGCCGCTGTG CTCGAGGGCG ATGTTATATT GGGCGGCCTG 
ATGATGGTCC ACTCGCGCGA GGACAGCATC ACTTGCGGAC CCATAATGCC GCAGGGCGGC 
ATCCAGGCCT TGGAGGCGAT GCTATACACC TTGGACCAGG TGAACAAGCA ACAGCTGCTG 
CCGAACGTCA CTCTGGGCGC CCACCTGCTC GACGATTGCG ACAAGGACAC CTACGGCCTG 
GAAATGGCAG TCGACTTTAT TAAAGGGTCC ATCAGCAACA TAGATGACGC GGAGTATCAC 
TGCAATAAGA CGCAGGTGCG CAAGGTCATC TCGGGGGTGG TGGGTGCCGC CTCCTCGGTC 
ACATCCATAC AAGTTGCCAA TTTATTGCGG TTATTCCGAA TTCCCCAGGT GTCGTACTTC 
TCCACCAGCC CCGAGCTGAG CAACAAACAG CGATTCGAGT ACTTCTCCCG CACTATTCCC 
TCGGATCACT ACCAGGTGAA GGCCATGGTG GAGATAGTGA AGCGGATGGG ATGGAGCTAC 
GTGTCGATTA TCTACGAGGA GAGCAATTAC GGAATCAAGG CTTTCGAGGA ACTGGAGGAG 
CTCCTCGCAC GCCATAACAT TTGTATTGCC ATCAAGGAGA AGCTGGTGAA GGATTCGGGA 
GTGGCCGAGG ACATCGCCTA CGACAATATA GTGCAGAAGC TGCTGACGAA GCCGCGGGCT 
CGAGGGGCCA TTATCTTCGG TTCGGATCAA GAGGTGCGCC AAGTGATGCG GGCGGTGCGC 
CGGGCGAATG CAACTGGTTC CTTCTCCTGG ATCGGATCCG ATGGCTGGAG TGCCCGGAAT 
TTGGTGTCCG ACGACTACGA GCCGGAGGTG GAAGGCACGC TGTCTGTGCA GCCGCAGGCG 
AATCCGGTGC GCGGGTTCGA GGAGTACTTC CTCAGTCTGA CGGTGGAGAA CAATCAACGC 
AATCCCTGGT TTGTGGAATT CTGGGAGGAT CACTTTCAGT GCCGATATCC CGGCAGTACG 
AGCACTCCGT ACAATAACTA CACCAAACAG TGCACCACAA AGGAGCGACT TTCACGCCAG 
AACACTGACT TCGAGGACCA GTTGCAGTTT GTCAGCGATG CGGTGATGGC ATTTGCATAC 
GCCCTAAGGG ATATGCACCG CGACCTGTGC GGCGGAGGTC CTTCGCTCTG CGAGGCCATG 
AAGCCAACGA AGGGTGCAGA CTTGCTGAAA TATTTGCGAA AAGTGGAGTT TGAGGGCCTC 
AGCGGCGACG AGTTCCGCTT CGACGGGAAC GGCGACGGTC CCGCCCGGTA CAACATCATC 
CACTTCAAGC AGTCCCAGGC GGGACAGTAC CACTGGGTCA AGGTGGGCGA GTACACCGAG 
GGCGAGCTGC GGCTTAATAT GACGGAGGTG AAGTTTAAGC GGCTGAGTCC CAAGCCGCCG 
GAGTCTGTCT GCAGCCTGCC ATGTCTTGTG GGTCAGGCCA AGAAGTACGT GGAAGGCGAG 
AGCTGCTGTT GGCACTGCTT CAATTGCACA ACCTATCAAA TCCGCCACCC GGATGACGAG 
ACCCATTGCA AGCTGTGCAA GCTGGGCACT CTTCCCGACG CCCACAAGCA GTACTGCCGC 
CCCATCCCGG AGATATATTT GCGTCCGGAG TCCGCTTGGG CCATCGGTGC TATGGCGTTC 
AGTGCGACCG GGATCTTAGT AACGCTCTTC GTAATGGGCG TATTTGTCAG GCACAACGAC 
ACGCCCATAG TGCGTGCCTC TGGTCGGGAG CTGAGCTACA TCCTGCTGGC GGGGATTTTC 
ATGTGCTACG GTGTCACCTT TGCCCTGGTC CTGAAGCCGA CCAACATAGT GTGTGCCATT 
CAGCGGTTTG GTGTCGGCTT CTGCTTCACG GTCGTTTATG CAGCACTGTT GACCAAAACG 
AATCGCATTG CACGGATCTT CAAAGCGGGC AAACAATCCG CCAAGAGGCC CTCCTTCATT 
AGTCCCAAGT CCCAGCTGGT AATCTGTGCG TGCCTGGTTA GTGTGCAGAT ACTCATCAAC 
GGCGTTTGGA TGGTAATTGC GCCATCACAT GCCATGCACC ATTATCCCAC ACGCGAGGAT 
AATCTGCTCG TCTGCGACTC GTACATCGAC GCCTCCTACA TGATTGCGTT CTCCTACCCA 
ATCTTCCTGA TTGTTATTTG CACGGTCTAC GCGGTGCTCA CGCGAAAGAT TCCGGAGGCT 
TTCAACGAAT CGAAGCATAT TGGATTCACC ATGTACACGA CTTGTGTGAT TTGGCTGGCT 
TTTGTACCGC TCTACTTCGG AACCGCCAAC CACGTTCCGC TGCGCATCAC CAGCATGTCG 
GTGACCATCA GCTTATCCGC CAGCGTGACC ATCGCCTGTC TCTTTTCGCC CAAGCTGTAC 
ATAATACTCA TACGCCCCGA GCGCAACGTG CGCCAGAGTA TGATGCCGCC TCGCTACGGC 
AACATGCACC GCACCGCTGG CACAGGACCC TCGTCCATGA TGGCCGCCGC CGTGGTGACC 
GCCGCCACCT GTGCCCAGGA GGAGAAGATC CAGAAGCATA TCACTCCCAC AAACACAGAA 
AACTCGCTAA AGACGAAGAA ATTGTGCGAA ATGGCCACTC AGACAATTTC CAGTATAATC 
ACGTCGCTGG ACATCAATGC GTACAATCAA ATACCATATG CCGATCAATA TGTGCCAAAC 
AATGCCACAA CGGCCACGAC CACGCCCACA GATAAGGGCA GTGGCAACGG CAACAGCAAC 
AGCAATAGCA ATACGAATGG TAGTAGCAAT AGCAACAGCA ACAGCAATGG CGATGGCGAT 
GTGGCAACAG AGCAAGTCGT GGCCAACAAT AATAAAATCA ATCAGCGACA ACATGGCCAG 
CCGGCCGTGG CATTTGCCAT CACATCGACT AACAATCACA TAAGCGGCCC AGCAGCAACC 
ATAACAACAA CAGCGGCAAC TGGCGAAACA ACAACTGAGG CGCTGGTGGC TACAATAACA 
ACTCCATTGG CAACGGTGGA TGGCAGCGAA ACCATGTCCG TGGCTCCCAA CAATGGGCAT 
GGAAACAACG GGAGCCAACG TCCGCCGGTC CTGCAAACAA ACTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mtt ( NM_165609.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724683.2 Drosophila melanogaster mangetout (mtt), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa12774
Clone ID Related Accession (Same CDS sequence)	NM_165609.3
Accession Version	NM_165609.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mangetout, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165609.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAACAT TCTGTGACGA AAGCAAATGG TCCGTGCAAA TTTGTTTGCT CCTGCTCCTG 
GTCGTTCTGT CCACTTGTCA CATCCTGCAG ACCGAAAGCC AGGTGGTCGG CGACATCGAA 
CAGGCGGACG ACGGGGTGGC GTCCAGTCAT CGTCGCAGTC GTAGTCACAG TGCCAGCCAT 
CAAGCTCACG TTGGTAATGG CAATAATCCT GGCTGCAAGC ACCTCCATCA TCGTCAGCAT 
CGCGGCAAAA GTCACGCCCG GAAGCACAGA CAGAGCCATT ACGAGATGCT CGAGCTGGAG 
CAGCGGCAGC AGCGGATGGG CAAGCACTGG CGTCAGTGGA TGAGCCACCT GCAGTCGCCG 
GATTATGCCA ACGATGACCC ATTGCCAGAT GGCAGGCGCT CCTCGCCGGC TAATGCAGAC 
CTTACCTACG ACCACTTCGA GCTGGAGGAC TACCAGCGAT ATGCTAATAT TGACCTAGCA 
TCGCCGCGCT CCGGTCGCCA TCGCGGTCGC CATCTGCCCC TGAACAACAT GAACCTAATG 
CTGCCGCTCA GCTCGGCGGA TGGTAGTGGC AAGTACCTGA GATCCCTGCC GGATTCGGTG 
AGCTTCCAAA ACGATCGATT GTACGCCACC CAGCCGCAAA GGCACTGGCC CGTCAAGCGG 
GAGGCTATCG TGGAGGGCGA CGTCATTCTG GGCGGTCTGA TGATGGTCCA CTCGCGCGAG 
GACAGCATCA CCTGCGGTCC CATCATGCCG CAGGGTGGCA TCCAGGCCCT GGAGGCCATG 
CTCTTCACTA TCGACAGGAT AAACCGGGAG CAGCTCCTGC CGAACATTAC CCTGGGTGCC 
CATATCCTCG ACGACTGCGA TAAGGACTCG TATGGCCTTG AAATGGCCGT GGATTTTATC 
AAAGGGTCCA TCAGCAACAT AGATGACGCG GAGTATCACT GCAATAAGAC GCAGGTGCGC 
AAGGTCATCT CGGGGGTGGT GGGTGCCGCC TCCTCGGTCA CATCCATACA AGTTGCCAAT 
TTATTGCGGT TATTCCGAAT TCCCCAGGTG TCGTACTTCT CCACCAGCCC CGAGCTGAGC 
AACAAACAGC GATTCGAGTA CTTCTCCCGC ACTATTCCCT CGGATCACTA CCAGGTGAAG 
GCCATGGTGG AGATAGTGAA GCGGATGGGA TGGAGCTACG TGTCGATTAT CTACGAGGAG 
AGCAATTACG GAATCAAGGC TTTCGAGGAA CTGGAGGAGC TCCTCGCACG CCATAACATT 
TGTATTGCCA TCAAGGAGAA GCTGGTGAAG GATTCGGGAG TGGCCGAGGA CATCGCCTAC 
GACAATATAG TGCAGAAGCT GCTGACGAAG CCGCGGGCTC GAGGGGCCAT TATCTTCGGT 
TCGGATCAAG AGGTGCGCCA AGTGATGCGG GCGGTGCGCC GGGCGAATGC AACTGGTTCC 
TTCTCCTGGA TCGGATCCGA TGGCTGGAGT GCCCGGAATT TGGTGTCCGA CGACTACGAG 
CCGGAGGTGG AAGGCACGCT GTCTGTGCAG CCGCAGGCGA ATCCGGTGCG CGGGTTCGAG 
GAGTACTTCC TCAGTCTGAC GGTGGAGAAC AATCAACGCA ATCCCTGGTT TGTGGAATTC 
TGGGAGGATC ACTTTCAGTG CCGATATCCC GGCAGTACGA GCACTCCGTA CAATAACTAC 
ACCAAACAGT GCACCACAAA GGAGCGACTT TCACGCCAGA ACACTGACTT CGAGGACCAG 
TTGCAGTTTG TCAGCGATGC GGTGATGGCA TTTGCATACG CCCTAAGGGA TATGCACCGC 
GACCTGTGCG GCGGAGGTCC TTCGCTCTGC GAGGCCATGA AGCCAACGAA GGGTGCAGAC 
TTGCTGAAAT ATTTGCGAAA AGTGGAGTTT GAGGGCCTCA GCGGCGACGA GTTCCGCTTC 
GACGGGAACG GCGACGGTCC CGCCCGGTAC AACATCATCC ACTTCAAGCA GTCCCAGGCG 
GGACAGTACC ACTGGGTCAA GGTGGGCGAG TACACCGAGG GCGAGCTGCG GCTTAATATG 
ACGGAGGTGA AGTTTAAGCG GCTGAGTCCC AAGCCGCCGG AGTCTGTCTG CAGCCTGCCA 
TGTCTTGTGG GTCAGGCCAA GAAGTACGTG GAAGGCGAGA GCTGCTGTTG GCACTGCTTC 
AATTGCACAA CCTATCAAAT CCGCCACCCG GATGACGAGA CCCATTGCAA GCTGTGCAAG 
CTGGGCACTC TTCCCGACGC CCACAAGCAG TACTGCCGCC CCATCCCGGA GATATATTTG 
CGTCCGGAGT CCGCTTGGGC CATCGGTGCT ATGGCGTTCA GTGCGACCGG GATCTTAGTA 
ACGCTCTTCG TAATGGGCGT ATTTGTCAGG CACAACGACA CGCCCATAGT GCGTGCCTCT 
GGTCGGGAGC TGAGCTACAT CCTGCTGGCG GGGATTTTCA TGTGCTACGG TGTCACCTTT 
GCCCTGGTCC TGAAGCCGAC CAACATAGTG TGTGCCATTC AGCGGTTTGG TGTCGGCTTC 
TGCTTCACGG TCGTTTATGC AGCACTGTTG ACCAAAACGA ATCGCATTGC ACGGATCTTC 
AAAGCGGGCA AACAATCCGC CAAGAGGCCC TCCTTCATTA GTCCCAAGTC CCAGCTGGTA 
ATCTGTGCGT GCCTGGTTAG TGTGCAGATA CTCATCAACG GCGTTTGGAT GGTAATTGCG 
CCATCACATG CCATGCACCA TTATCCCACA CGCGAGGATA ATCTGCTCGT CTGCGACTCG 
TACATCGACG CCTCCTACAT GATTGCGTTC TCCTACCCAA TCTTCCTGAT TGTTATTTGC 
ACGGTCTACG CGGTGCTCAC GCGAAAGATT CCGGAGGCTT TCAACGAATC GAAGCATATT 
GGATTCACCA TGTACACGAC TTGTGTGATT TGGCTGGCTT TTGTACCGCT CTACTTCGGA 
ACCGCCAACC ACGTTCCGCT GCGCATCACC AGCATGTCGG TGACCATCAG CTTATCCGCC 
AGCGTGACCA TCGCCTGTCT CTTTTCGCCC AAGCTGTACA TAATACTCAT ACGCCCCGAG 
CGCAACGTGC GCCAGAGTAT GATGCCGCCT CGCTACGGCA ACATGCACCG CACCGCTGGC 
ACAGGACCCT CGTCCATGAT GGCCGCCGCC GTGGTGACCG CCGCCACCTG TGCCCAGGAG 
GAGAAGATCC AGAAGCATAT CACTCCCACA AACACAGAAA ACTCGCTAAA GACGAAGAAA 
TTGTGCGAAA TGGCCACTCA GACAATTTCC AGTATAATCA CGTCGCTGGA CATCAATGCG 
TACAATCAAA TACCATATGC CGATCAATAT GTGCCAAACA ATGCCACAAC GGCCACGACC 
ACGCCCACAG ATAAGGGCAG TGGCAACGGC AACAGCAACA GCAATAGCAA TACGAATGGT 
AGTAGCAATA GCAACAGCAA CAGCAATGGC GATGGCGATG TGGCAACAGA GCAAGTCGTG 
GCCAACAATA ATAAAATCAA TCAGCGACAA CATGGCCAGC CGGCCGTGGC ATTTGCCATC 
ACATCGACTA ACAATCACAT AAGCGGCCCA GCAGCAACCA TAACAACAAC AGCGGCAACT 
GGCGAAACAA CAACTGAGGC GCTGGTGGCT ACAATAACAA CTCCATTGGC AACGGTGGAT 
GGCAGCGAAA CCATGTCCGT GGCTCCCAAC AATGGGCATG GAAACAACGG GAGCCAACGT 
CCGCCGGTCC TGCAAACAAA CTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724683.2
CDS	217..3963
Translation	MKTFCDESKWSVQICLLLLLVVLSTCHILQTESQVVGDIEQADDGVASSHRRSRSHSASHQAHVGNGNNPGCKHLHHRQHRGKSHARKHRQSHYEMLELEQRQQRMGKHWRQWMSHLQSPDYANDDPLPDGRRSSPANADLTYDHFELEDYQRYANIDLASPRSGRHRGRHLPLNNMNLMLPLSSADGSGKYLRSLPDSVSFQNDRLYATQPQRHWPVKREAIVEGDVILGGLMMVHSREDSITCGPIMPQGGIQALEAMLFTIDRINREQLLPNITLGAHILDDCDKDSYGLEMAVDFIKGSISNIDDAEYHCNKTQVRKVISGVVGAASSVTSIQVANLLRLFRIPQVSYFSTSPELSNKQRFEYFSRTIPSDHYQVKAMVEIVKRMGWSYVSIIYEESNYGIKAFEELEELLARHNICIAIKEKLVKDSGVAEDIAYDNIVQKLLTKPRARGAIIFGSDQEVRQVMRAVRRANATGSFSWIGSDGWSARNLVSDDYEPEVEGTLSVQPQANPVRGFEEYFLSLTVENNQRNPWFVEFWEDHFQCRYPGSTSTPYNNYTKQCTTKERLSRQNTDFEDQLQFVSDAVMAFAYALRDMHRDLCGGGPSLCEAMKPTKGADLLKYLRKVEFEGLSGDEFRFDGNGDGPARYNIIHFKQSQAGQYHWVKVGEYTEGELRLNMTEVKFKRLSPKPPESVCSLPCLVGQAKKYVEGESCCWHCFNCTTYQIRHPDDETHCKLCKLGTLPDAHKQYCRPIPEIYLRPESAWAIGAMAFSATGILVTLFVMGVFVRHNDTPIVRASGRELSYILLAGIFMCYGVTFALVLKPTNIVCAIQRFGVGFCFTVVYAALLTKTNRIARIFKAGKQSAKRPSFISPKSQLVICACLVSVQILINGVWMVIAPSHAMHHYPTREDNLLVCDSYIDASYMIAFSYPIFLIVICTVYAVLTRKIPEAFNESKHIGFTMYTTCVIWLAFVPLYFGTANHVPLRITSMSVTISLSASVTIACLFSPKLYIILIRPERNVRQSMMPPRYGNMHRTAGTGPSSMMAAAVVTAATCAQEEKIQKHITPTNTENSLKTKKLCEMATQTISSIITSLDINAYNQIPYADQYVPNNATTATTTPTDKGSGNGNSNSNSNTNGSSNSNSNSNGDGDVATEQVVANNNKINQRQHGQPAVAFAITSTNNHISGPAATITTTAATGETTTEALVATITTPLATVDGSETMSVAPNNGHGNNGSQRPPVLQTNL

Target ORF information:

RefSeq Version	NM_165609.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mangetout (mtt), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165609.3

ORF Insert Sequence:

ATGAAAACAT TCTGTGACGA AAGCAAATGG TCCGTGCAAA TTTGTTTGCT CCTGCTCCTG 
GTCGTTCTGT CCACTTGTCA CATCCTGCAG ACCGAAAGCC AGGTGGTCGG CGACATCGAA 
CAGGCGGACG ACGGGGTGGC GTCCAGTCAT CGTCGCAGTC GTAGTCACAG TGCCAGCCAT 
CAAGCTCACG TTGGTAATGG CAATAATCCT GGCTGCAAGC ACCTCCATCA TCGTCAGCAT 
CGCGGCAAAA GTCACGCCCG GAAGCACAGA CAGAGCCATT ACGAGATGCT CGAGCTGGAG 
CAGCGGCAGC AGCGGATGGG CAAGCACTGG CGTCAGTGGA TGAGCCACCT GCAGTCGCCG 
GATTATGCCA ACGATGACCC ATTGCCAGAT GGCAGGCGCT CCTCGCCGGC TAATGCAGAC 
CTTACCTACG ACCACTTCGA GCTGGAGGAC TACCAGCGAT ATGCTAATAT TGACCTAGCA 
TCGCCGCGCT CCGGTCGCCA TCGCGGTCGC CATCTGCCCC TGAACAACAT GAACCTAATG 
CTGCCGCTCA GCTCGGCGGA TGGTAGTGGC AAGTACCTGA GATCCCTGCC GGATTCGGTG 
AGCTTCCAAA ACGATCGATT GTACGCCACC CAGCCGCAAA GGCACTGGCC CGTCAAGCGG 
GAGGCTATCG TGGAGGGCGA CGTCATTCTG GGCGGTCTGA TGATGGTCCA CTCGCGCGAG 
GACAGCATCA CCTGCGGTCC CATCATGCCG CAGGGTGGCA TCCAGGCCCT GGAGGCCATG 
CTCTTCACTA TCGACAGGAT AAACCGGGAG CAGCTCCTGC CGAACATTAC CCTGGGTGCC 
CATATCCTCG ACGACTGCGA TAAGGACTCG TATGGCCTTG AAATGGCCGT GGATTTTATC 
AAAGGGTCCA TCAGCAACAT AGATGACGCG GAGTATCACT GCAATAAGAC GCAGGTGCGC 
AAGGTCATCT CGGGGGTGGT GGGTGCCGCC TCCTCGGTCA CATCCATACA AGTTGCCAAT 
TTATTGCGGT TATTCCGAAT TCCCCAGGTG TCGTACTTCT CCACCAGCCC CGAGCTGAGC 
AACAAACAGC GATTCGAGTA CTTCTCCCGC ACTATTCCCT CGGATCACTA CCAGGTGAAG 
GCCATGGTGG AGATAGTGAA GCGGATGGGA TGGAGCTACG TGTCGATTAT CTACGAGGAG 
AGCAATTACG GAATCAAGGC TTTCGAGGAA CTGGAGGAGC TCCTCGCACG CCATAACATT 
TGTATTGCCA TCAAGGAGAA GCTGGTGAAG GATTCGGGAG TGGCCGAGGA CATCGCCTAC 
GACAATATAG TGCAGAAGCT GCTGACGAAG CCGCGGGCTC GAGGGGCCAT TATCTTCGGT 
TCGGATCAAG AGGTGCGCCA AGTGATGCGG GCGGTGCGCC GGGCGAATGC AACTGGTTCC 
TTCTCCTGGA TCGGATCCGA TGGCTGGAGT GCCCGGAATT TGGTGTCCGA CGACTACGAG 
CCGGAGGTGG AAGGCACGCT GTCTGTGCAG CCGCAGGCGA ATCCGGTGCG CGGGTTCGAG 
GAGTACTTCC TCAGTCTGAC GGTGGAGAAC AATCAACGCA ATCCCTGGTT TGTGGAATTC 
TGGGAGGATC ACTTTCAGTG CCGATATCCC GGCAGTACGA GCACTCCGTA CAATAACTAC 
ACCAAACAGT GCACCACAAA GGAGCGACTT TCACGCCAGA ACACTGACTT CGAGGACCAG 
TTGCAGTTTG TCAGCGATGC GGTGATGGCA TTTGCATACG CCCTAAGGGA TATGCACCGC 
GACCTGTGCG GCGGAGGTCC TTCGCTCTGC GAGGCCATGA AGCCAACGAA GGGTGCAGAC 
TTGCTGAAAT ATTTGCGAAA AGTGGAGTTT GAGGGCCTCA GCGGCGACGA GTTCCGCTTC 
GACGGGAACG GCGACGGTCC CGCCCGGTAC AACATCATCC ACTTCAAGCA GTCCCAGGCG 
GGACAGTACC ACTGGGTCAA GGTGGGCGAG TACACCGAGG GCGAGCTGCG GCTTAATATG 
ACGGAGGTGA AGTTTAAGCG GCTGAGTCCC AAGCCGCCGG AGTCTGTCTG CAGCCTGCCA 
TGTCTTGTGG GTCAGGCCAA GAAGTACGTG GAAGGCGAGA GCTGCTGTTG GCACTGCTTC 
AATTGCACAA CCTATCAAAT CCGCCACCCG GATGACGAGA CCCATTGCAA GCTGTGCAAG 
CTGGGCACTC TTCCCGACGC CCACAAGCAG TACTGCCGCC CCATCCCGGA GATATATTTG 
CGTCCGGAGT CCGCTTGGGC CATCGGTGCT ATGGCGTTCA GTGCGACCGG GATCTTAGTA 
ACGCTCTTCG TAATGGGCGT ATTTGTCAGG CACAACGACA CGCCCATAGT GCGTGCCTCT 
GGTCGGGAGC TGAGCTACAT CCTGCTGGCG GGGATTTTCA TGTGCTACGG TGTCACCTTT 
GCCCTGGTCC TGAAGCCGAC CAACATAGTG TGTGCCATTC AGCGGTTTGG TGTCGGCTTC 
TGCTTCACGG TCGTTTATGC AGCACTGTTG ACCAAAACGA ATCGCATTGC ACGGATCTTC 
AAAGCGGGCA AACAATCCGC CAAGAGGCCC TCCTTCATTA GTCCCAAGTC CCAGCTGGTA 
ATCTGTGCGT GCCTGGTTAG TGTGCAGATA CTCATCAACG GCGTTTGGAT GGTAATTGCG 
CCATCACATG CCATGCACCA TTATCCCACA CGCGAGGATA ATCTGCTCGT CTGCGACTCG 
TACATCGACG CCTCCTACAT GATTGCGTTC TCCTACCCAA TCTTCCTGAT TGTTATTTGC 
ACGGTCTACG CGGTGCTCAC GCGAAAGATT CCGGAGGCTT TCAACGAATC GAAGCATATT 
GGATTCACCA TGTACACGAC TTGTGTGATT TGGCTGGCTT TTGTACCGCT CTACTTCGGA 
ACCGCCAACC ACGTTCCGCT GCGCATCACC AGCATGTCGG TGACCATCAG CTTATCCGCC 
AGCGTGACCA TCGCCTGTCT CTTTTCGCCC AAGCTGTACA TAATACTCAT ACGCCCCGAG 
CGCAACGTGC GCCAGAGTAT GATGCCGCCT CGCTACGGCA ACATGCACCG CACCGCTGGC 
ACAGGACCCT CGTCCATGAT GGCCGCCGCC GTGGTGACCG CCGCCACCTG TGCCCAGGAG 
GAGAAGATCC AGAAGCATAT CACTCCCACA AACACAGAAA ACTCGCTAAA GACGAAGAAA 
TTGTGCGAAA TGGCCACTCA GACAATTTCC AGTATAATCA CGTCGCTGGA CATCAATGCG 
TACAATCAAA TACCATATGC CGATCAATAT GTGCCAAACA ATGCCACAAC GGCCACGACC 
ACGCCCACAG ATAAGGGCAG TGGCAACGGC AACAGCAACA GCAATAGCAA TACGAATGGT 
AGTAGCAATA GCAACAGCAA CAGCAATGGC GATGGCGATG TGGCAACAGA GCAAGTCGTG 
GCCAACAATA ATAAAATCAA TCAGCGACAA CATGGCCAGC CGGCCGTGGC ATTTGCCATC 
ACATCGACTA ACAATCACAT AAGCGGCCCA GCAGCAACCA TAACAACAAC AGCGGCAACT 
GGCGAAACAA CAACTGAGGC GCTGGTGGCT ACAATAACAA CTCCATTGGC AACGGTGGAT 
GGCAGCGAAA CCATGTCCGT GGCTCCCAAC AATGGGCATG GAAACAACGG GAGCCAACGT 
CCGCCGGTCC TGCAAACAAA CTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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mtt cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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