Pbp49 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pbp49
Entrez Gene ID	35787
Full Name	PSEA-binding protein 49kD
Synonyms	CG11508,CG42515,DMPBP49,DmPBP,DmPBP49,DmSNAP50,DmSNAPc,Dmel\CG42515,Dmel_CG11508,Dromel_CG11508_FBtr0088817_mORF
General protein information	Preferred Names PSEA-binding protein 49kD Names CG42515 gene product from transcript CG42515-RB CG42515-PA CG42515-PB CG42515-PD PSEA-binding protein PSEA-binding protein of 49 kDa Pbp49-PA Pbp49-PB Pbp49-PD drosophila melanogaster PSE-binding protein 49-kDa subunit proximal sequence Element-Binding protein 49 snRNA activator protein 50
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 44B3-44B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_165588.4	NP_724647.1	PSEA-binding protein 49kD, isoform B
NM_001144144.2	NP_001137616.2	PSEA-binding protein 49kD, isoform D
NM_136513.4	NP_610357.1	PSEA-binding protein 49kD, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data help to establish the orientation of each SNAPc subunit on DNA and reveal cases in which different domains of the subunits SNAP43, 50, and 190 differentially contact the U1 versus U6 proximal sequence elements A.
Title: Identification of SNAPc subunit domains that interact with specific nucleotide positions in the U1 and U6 gene promoters.

Data suggests that the non-conserved region of DmSNAP50 (residues 1-109) makes little if any contribution toward forming a complex with DmSNAP190 and DmSNAP43.
Title: A map of Drosophila melanogaster small nuclear RNA-activating protein complex (DmSNAPc) domains involved in subunit assembly and DNA binding.

Results support a model in which conformational differences in proximal sequence element binding protein (DmPBP)-DNA complexes lead to selective RNA polymerase recruitment to U1 and U6 promoters.
Title: Architectural arrangement of cloned proximal sequence element-binding protein subunits on Drosophila U1 and U6 snRNA gene promoters.

The following Pbp49 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pbp49 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa09258	NM_136513.4 Latest version!	Drosophila melanogaster PSEA-binding protein 49kD (Pbp49), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa09258	NM_165588.4 Latest version!	Drosophila melanogaster PSEA-binding protein 49kD (Pbp49), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa21706	NM_001144144.2 Latest version!	Drosophila melanogaster PSEA-binding protein 49kD (Pbp49), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pbp49 ( NM_165588.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724647.1 Drosophila melanogaster PSEA-binding protein 49kD (Pbp49), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa09258
Clone ID Related Accession (Same CDS sequence)	NM_165588.4 , NM_136513.4
Accession Version	NM_165588.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	PSEA-binding protein 49kD, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_165588.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATACCA CCTATATTAC AACGAATCCA GCTTTAAGCC TGCGAGACTT TCTAGCCGAC 
TATCAAAAGA AAATATCCGG TACTGCAGAC GAAGTGCCCT TCTTTCTCCA AAAAAACCCA 
GTACTTGTCG ACGTGTCTGA AAGCTGTTCC CTGGACTTGA TCGAGTCCCC AGACGACAGC 
TCGATTGCAG TCTTCCAGCC CGCCGCAGAC AAATGCCGCC CAACCTTTTC TCCGCCGCAG 
GAGAACCAGC ATGTGCCCTC CACTTTTACT GCTCTAAGCC AGCACAAGGA CAAGTCCCGT 
AAGTGTCCCT TTGGCCGCAC CCAGTACAGC CACAAACTGA ATCCGACGCC GACAGACTCG 
CCTAACCTGC ACATCGATGC TTGCGGCGAA CTGGAGCTAA CCGTGCGTCT ATATCGACCA 
CCGCGGGCCT ACCATCGCGG GTTTAAGGTG GAGATTCCCG TTTTCGCCGA GGAGTTCGTA 
TGTCTGGGCA GCAATTATCT GACCGAGCTG CGGGACAAGA TCAGCTGCGT TTGCAATGGA 
AAACGGTTTG TCGACATCAG CGACGATCCA GATGCACCGT TGCCATCCAT TGACACAAAT 
CCCGGTTACT TCTTCATCAA CGACACATTC TACAACGACC AGCGCAATCC GGACAATCCC 
GACTATTCCA AGACCGTCTT GCAGTGGGCT GCCAGAGCGA ACGGAGTGAA CGGAGAAACG 
CTTAAGGTGG AGAGTATGGA GGGCAAAAGA TTCATCGATC TCACTGTCAG CCCCGGATCA 
CCGCTACACT ACCTGCACCA CGGCAATTGC GAACACCTGT TTGTGATCTC CCAGGTAGAG 
GTGCTAACGC CACTTAGTAA ACGGCCAGAT CGCAGCCTGT ATCCCTATCC TCACGCCTTC 
AGTACGTTTA ATCGCAGGAC TTGCTATATG TGCGGCATTC GCAGCTATAG CTTCATCGTG 
AACCAGTCCC GGCGACAGCT GCACGATCCC TCCTACCTGT GCCGCAGATG TTTTCTCAGC 
TTCTTTTACG TGGATGGTGT CAAGCTGGGC CAGTTCAAAG CCTATCGCAT GTATGACCAT 
GTAGAGCTGG AGGGCGAAGA GGAGGACATT AAACAGCAAA ATCTCCCCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724647.1
CDS	613..1746
Translation	MDTTYITTNPALSLRDFLADYQKKISGTADEVPFFLQKNPVLVDVSESCSLDLIESPDDSSIAVFQPAADKCRPTFSPPQENQHVPSTFTALSQHKDKSRKCPFGRTQYSHKLNPTPTDSPNLHIDACGELELTVRLYRPPRAYHRGFKVEIPVFAEEFVCLGSNYLTELRDKISCVCNGKRFVDISDDPDAPLPSIDTNPGYFFINDTFYNDQRNPDNPDYSKTVLQWAARANGVNGETLKVESMEGKRFIDLTVSPGSPLHYLHHGNCEHLFVISQVEVLTPLSKRPDRSLYPYPHAFSTFNRRTCYMCGIRSYSFIVNQSRRQLHDPSYLCRRCFLSFFYVDGVKLGQFKAYRMYDHVELEGEEEDIKQQNLPS

Target ORF information:

RefSeq Version	NM_165588.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster PSEA-binding protein 49kD (Pbp49), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165588.4

ORF Insert Sequence:

ATGGATACCA CCTATATTAC AACGAATCCA GCTTTAAGCC TGCGAGACTT TCTAGCCGAC 
TATCAAAAGA AAATATCCGG TACTGCAGAC GAAGTGCCCT TCTTTCTCCA AAAAAACCCA 
GTACTTGTCG ACGTGTCTGA AAGCTGTTCC CTGGACTTGA TCGAGTCCCC AGACGACAGC 
TCGATTGCAG TCTTCCAGCC CGCCGCAGAC AAATGCCGCC CAACCTTTTC TCCGCCGCAG 
GAGAACCAGC ATGTGCCCTC CACTTTTACT GCTCTAAGCC AGCACAAGGA CAAGTCCCGT 
AAGTGTCCCT TTGGCCGCAC CCAGTACAGC CACAAACTGA ATCCGACGCC GACAGACTCG 
CCTAACCTGC ACATCGATGC TTGCGGCGAA CTGGAGCTAA CCGTGCGTCT ATATCGACCA 
CCGCGGGCCT ACCATCGCGG GTTTAAGGTG GAGATTCCCG TTTTCGCCGA GGAGTTCGTA 
TGTCTGGGCA GCAATTATCT GACCGAGCTG CGGGACAAGA TCAGCTGCGT TTGCAATGGA 
AAACGGTTTG TCGACATCAG CGACGATCCA GATGCACCGT TGCCATCCAT TGACACAAAT 
CCCGGTTACT TCTTCATCAA CGACACATTC TACAACGACC AGCGCAATCC GGACAATCCC 
GACTATTCCA AGACCGTCTT GCAGTGGGCT GCCAGAGCGA ACGGAGTGAA CGGAGAAACG 
CTTAAGGTGG AGAGTATGGA GGGCAAAAGA TTCATCGATC TCACTGTCAG CCCCGGATCA 
CCGCTACACT ACCTGCACCA CGGCAATTGC GAACACCTGT TTGTGATCTC CCAGGTAGAG 
GTGCTAACGC CACTTAGTAA ACGGCCAGAT CGCAGCCTGT ATCCCTATCC TCACGCCTTC 
AGTACGTTTA ATCGCAGGAC TTGCTATATG TGCGGCATTC GCAGCTATAG CTTCATCGTG 
AACCAGTCCC GGCGACAGCT GCACGATCCC TCCTACCTGT GCCGCAGATG TTTTCTCAGC 
TTCTTTTACG TGGATGGTGT CAAGCTGGGC CAGTTCAAAG CCTATCGCAT GTATGACCAT 
GTAGAGCTGG AGGGCGAAGA GGAGGACATT AAACAGCAAA ATCTCCCCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbp49 ( NM_136513.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610357.1 Drosophila melanogaster PSEA-binding protein 49kD (Pbp49), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa09258
Clone ID Related Accession (Same CDS sequence)	NM_165588.4 , NM_136513.4
Accession Version	NM_136513.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	PSEA-binding protein 49kD, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_136513.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATACCA CCTATATTAC AACGAATCCA GCTTTAAGCC TGCGAGACTT TCTAGCCGAC 
TATCAAAAGA AAATATCCGG TACTGCAGAC GAAGTGCCCT TCTTTCTCCA AAAAAACCCA 
GTACTTGTCG ACGTGTCTGA AAGCTGTTCC CTGGACTTGA TCGAGTCCCC AGACGACAGC 
TCGATTGCAG TCTTCCAGCC CGCCGCAGAC AAATGCCGCC CAACCTTTTC TCCGCCGCAG 
GAGAACCAGC ATGTGCCCTC CACTTTTACT GCTCTAAGCC AGCACAAGGA CAAGTCCCGT 
AAGTGTCCCT TTGGCCGCAC CCAGTACAGC CACAAACTGA ATCCGACGCC GACAGACTCG 
CCTAACCTGC ACATCGATGC TTGCGGCGAA CTGGAGCTAA CCGTGCGTCT ATATCGACCA 
CCGCGGGCCT ACCATCGCGG GTTTAAGGTG GAGATTCCCG TTTTCGCCGA GGAGTTCGTA 
TGTCTGGGCA GCAATTATCT GACCGAGCTG CGGGACAAGA TCAGCTGCGT TTGCAATGGA 
AAACGGTTTG TCGACATCAG CGACGATCCA GATGCACCGT TGCCATCCAT TGACACAAAT 
CCCGGTTACT TCTTCATCAA CGACACATTC TACAACGACC AGCGCAATCC GGACAATCCC 
GACTATTCCA AGACCGTCTT GCAGTGGGCT GCCAGAGCGA ACGGAGTGAA CGGAGAAACG 
CTTAAGGTGG AGAGTATGGA GGGCAAAAGA TTCATCGATC TCACTGTCAG CCCCGGATCA 
CCGCTACACT ACCTGCACCA CGGCAATTGC GAACACCTGT TTGTGATCTC CCAGGTAGAG 
GTGCTAACGC CACTTAGTAA ACGGCCAGAT CGCAGCCTGT ATCCCTATCC TCACGCCTTC 
AGTACGTTTA ATCGCAGGAC TTGCTATATG TGCGGCATTC GCAGCTATAG CTTCATCGTG 
AACCAGTCCC GGCGACAGCT GCACGATCCC TCCTACCTGT GCCGCAGATG TTTTCTCAGC 
TTCTTTTACG TGGATGGTGT CAAGCTGGGC CAGTTCAAAG CCTATCGCAT GTATGACCAT 
GTAGAGCTGG AGGGCGAAGA GGAGGACATT AAACAGCAAA ATCTCCCCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610357.1
CDS	696..1829
Translation	MDTTYITTNPALSLRDFLADYQKKISGTADEVPFFLQKNPVLVDVSESCSLDLIESPDDSSIAVFQPAADKCRPTFSPPQENQHVPSTFTALSQHKDKSRKCPFGRTQYSHKLNPTPTDSPNLHIDACGELELTVRLYRPPRAYHRGFKVEIPVFAEEFVCLGSNYLTELRDKISCVCNGKRFVDISDDPDAPLPSIDTNPGYFFINDTFYNDQRNPDNPDYSKTVLQWAARANGVNGETLKVESMEGKRFIDLTVSPGSPLHYLHHGNCEHLFVISQVEVLTPLSKRPDRSLYPYPHAFSTFNRRTCYMCGIRSYSFIVNQSRRQLHDPSYLCRRCFLSFFYVDGVKLGQFKAYRMYDHVELEGEEEDIKQQNLPS

Target ORF information:

RefSeq Version	NM_136513.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster PSEA-binding protein 49kD (Pbp49), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136513.4

ORF Insert Sequence:

ATGGATACCA CCTATATTAC AACGAATCCA GCTTTAAGCC TGCGAGACTT TCTAGCCGAC 
TATCAAAAGA AAATATCCGG TACTGCAGAC GAAGTGCCCT TCTTTCTCCA AAAAAACCCA 
GTACTTGTCG ACGTGTCTGA AAGCTGTTCC CTGGACTTGA TCGAGTCCCC AGACGACAGC 
TCGATTGCAG TCTTCCAGCC CGCCGCAGAC AAATGCCGCC CAACCTTTTC TCCGCCGCAG 
GAGAACCAGC ATGTGCCCTC CACTTTTACT GCTCTAAGCC AGCACAAGGA CAAGTCCCGT 
AAGTGTCCCT TTGGCCGCAC CCAGTACAGC CACAAACTGA ATCCGACGCC GACAGACTCG 
CCTAACCTGC ACATCGATGC TTGCGGCGAA CTGGAGCTAA CCGTGCGTCT ATATCGACCA 
CCGCGGGCCT ACCATCGCGG GTTTAAGGTG GAGATTCCCG TTTTCGCCGA GGAGTTCGTA 
TGTCTGGGCA GCAATTATCT GACCGAGCTG CGGGACAAGA TCAGCTGCGT TTGCAATGGA 
AAACGGTTTG TCGACATCAG CGACGATCCA GATGCACCGT TGCCATCCAT TGACACAAAT 
CCCGGTTACT TCTTCATCAA CGACACATTC TACAACGACC AGCGCAATCC GGACAATCCC 
GACTATTCCA AGACCGTCTT GCAGTGGGCT GCCAGAGCGA ACGGAGTGAA CGGAGAAACG 
CTTAAGGTGG AGAGTATGGA GGGCAAAAGA TTCATCGATC TCACTGTCAG CCCCGGATCA 
CCGCTACACT ACCTGCACCA CGGCAATTGC GAACACCTGT TTGTGATCTC CCAGGTAGAG 
GTGCTAACGC CACTTAGTAA ACGGCCAGAT CGCAGCCTGT ATCCCTATCC TCACGCCTTC 
AGTACGTTTA ATCGCAGGAC TTGCTATATG TGCGGCATTC GCAGCTATAG CTTCATCGTG 
AACCAGTCCC GGCGACAGCT GCACGATCCC TCCTACCTGT GCCGCAGATG TTTTCTCAGC 
TTCTTTTACG TGGATGGTGT CAAGCTGGGC CAGTTCAAAG CCTATCGCAT GTATGACCAT 
GTAGAGCTGG AGGGCGAAGA GGAGGACATT AAACAGCAAA ATCTCCCCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pbp49 ( NM_001144144.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137616.2 Drosophila melanogaster PSEA-binding protein 49kD (Pbp49), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa21706
Clone ID Related Accession (Same CDS sequence)	NM_001144144.2
Accession Version	NM_001144144.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	PSEA-binding protein 49kD, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001144144.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATACCA CCTATATTAC AACGAATCCA GCTTTAAGCC TGCGAGACTT TCTAGCCGAC 
TATCAAAAGA AAATATCCGG TACTGCAGAC GAAGTGCCCT TCTTTCTCCA AAAAAACCCA 
GTACTTGTCG ACGTGTCTGA AAGCTGTTCC CTGGACTTGA TCGAGTCCCC AGACGACAGC 
TCGATTGCAG TCTTCCAGCC CGCCGCAGAC AAATGCCGCC CAACCTTTTC TCCGCCGCAG 
GAGAACCAGC ATGTGCCCTC CACTTTTACT GCTCTAAGCC AGCACAAGGA CAAGTCCCAC 
TCGCCTAACC TGCACATCGA TGCTTGCGGC GAACTGGAGC TAACCGTGCG TCTATATCGA 
CCACCGCGGG CCTACCATCG CGGGTTTAAG GTGGAGATTC CCGTTTTCGC CGAGGAGTTC 
GTATGTCTGG GCAGCAATTA TCTGACCGAG CTGCGGGACA AGATCAGCTG CGTTTGCAAT 
GGAAAACGGT TTGTCGACAT CAGCGACGAT CCAGATGCAC CGTTGCCATC CATTGACACA 
AATCCCGGTT ACTTCTTCAT CAACGACACA TTCTACAACG ACCAGCGCAA TCCGGACAAT 
CCCGACTATT CCAAGACCGT CTTGCAGTGG GCTGCCAGAG CGAACGGAGT GAACGGAGAA 
ACGCTTAAGG TGGAGAGTAT GGAGGGCAAA AGATTCATCG ATCTCACTGT CAGCCCCGGA 
TCACCGCTAC ACTACCTGCA CCACGGCAAT TGCGAACACC TGTTTGTGAT CTCCCAGGTA 
GAGGTGCTAA CGCCACTTAG TAAACGGCCA GATCGCAGCC TGTATCCCTA TCCTCACGCC 
TTCAGTACGT TTAATCGCAG GACTTGCTAT ATGTGCGGCA TTCGCAGCTA TAGCTTCATC 
GTGAACCAGT CCCGGCGACA GCTGCACGAT CCCTCCTACC TGTGCCGCAG ATGTTTTCTC 
AGCTTCTTTT ACGTGGATGG TGTCAAGCTG GGCCAGTTCA AAGCCTATCG CATGTATGAC 
CATGTAGAGC TGGAGGGCGA AGAGGAGGAC ATTAAACAGC AAAATCTCCC CTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137616.2
CDS	696..1772
Translation	MDTTYITTNPALSLRDFLADYQKKISGTADEVPFFLQKNPVLVDVSESCSLDLIESPDDSSIAVFQPAADKCRPTFSPPQENQHVPSTFTALSQHKDKSHSPNLHIDACGELELTVRLYRPPRAYHRGFKVEIPVFAEEFVCLGSNYLTELRDKISCVCNGKRFVDISDDPDAPLPSIDTNPGYFFINDTFYNDQRNPDNPDYSKTVLQWAARANGVNGETLKVESMEGKRFIDLTVSPGSPLHYLHHGNCEHLFVISQVEVLTPLSKRPDRSLYPYPHAFSTFNRRTCYMCGIRSYSFIVNQSRRQLHDPSYLCRRCFLSFFYVDGVKLGQFKAYRMYDHVELEGEEEDIKQQNLPS

Target ORF information:

RefSeq Version	NM_001144144.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster PSEA-binding protein 49kD (Pbp49), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144144.2

ORF Insert Sequence:

ATGGATACCA CCTATATTAC AACGAATCCA GCTTTAAGCC TGCGAGACTT TCTAGCCGAC 
TATCAAAAGA AAATATCCGG TACTGCAGAC GAAGTGCCCT TCTTTCTCCA AAAAAACCCA 
GTACTTGTCG ACGTGTCTGA AAGCTGTTCC CTGGACTTGA TCGAGTCCCC AGACGACAGC 
TCGATTGCAG TCTTCCAGCC CGCCGCAGAC AAATGCCGCC CAACCTTTTC TCCGCCGCAG 
GAGAACCAGC ATGTGCCCTC CACTTTTACT GCTCTAAGCC AGCACAAGGA CAAGTCCCAC 
TCGCCTAACC TGCACATCGA TGCTTGCGGC GAACTGGAGC TAACCGTGCG TCTATATCGA 
CCACCGCGGG CCTACCATCG CGGGTTTAAG GTGGAGATTC CCGTTTTCGC CGAGGAGTTC 
GTATGTCTGG GCAGCAATTA TCTGACCGAG CTGCGGGACA AGATCAGCTG CGTTTGCAAT 
GGAAAACGGT TTGTCGACAT CAGCGACGAT CCAGATGCAC CGTTGCCATC CATTGACACA 
AATCCCGGTT ACTTCTTCAT CAACGACACA TTCTACAACG ACCAGCGCAA TCCGGACAAT 
CCCGACTATT CCAAGACCGT CTTGCAGTGG GCTGCCAGAG CGAACGGAGT GAACGGAGAA 
ACGCTTAAGG TGGAGAGTAT GGAGGGCAAA AGATTCATCG ATCTCACTGT CAGCCCCGGA 
TCACCGCTAC ACTACCTGCA CCACGGCAAT TGCGAACACC TGTTTGTGAT CTCCCAGGTA 
GAGGTGCTAA CGCCACTTAG TAAACGGCCA GATCGCAGCC TGTATCCCTA TCCTCACGCC 
TTCAGTACGT TTAATCGCAG GACTTGCTAT ATGTGCGGCA TTCGCAGCTA TAGCTTCATC 
GTGAACCAGT CCCGGCGACA GCTGCACGAT CCCTCCTACC TGTGCCGCAG ATGTTTTCTC 
AGCTTCTTTT ACGTGGATGG TGTCAAGCTG GGCCAGTTCA AAGCCTATCG CATGTATGAC 
CATGTAGAGC TGGAGGGCGA AGAGGAGGAC ATTAAACAGC AAAATCTCCC CTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Pbp49 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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