Cul4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cul4
Entrez Gene ID	35780
Full Name	Cullin 4
Synonyms	CG8711,CUL4,Cul-4,Cullin4,DMcul-4,Dmel\CG8711,cul-4,cul4,dCul4
General protein information	Preferred Names Cullin 4 Names CG8711 gene product from transcript CG8711-RA CG8711-PA CG8711-PB Cul4-PA Cul4-PB Cullin-4 Cullin4 cul-4 cullin 4
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 44A4-44A4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001169613.1	NP_001163084.1	cullin 4, isoform B
NM_136508.4	NP_610352.2	cullin 4, isoform A

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CUL4B	XP_864628.3
Mus musculus (house mouse)	Cul4b	NP_001103612.1
Danio rerio (zebrafish)	cul4b	NP_001116316.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007727	XP_308149.4
Homo sapiens (human)	CUL4B	NP_001073341.1
	CUL4B	XP_001086195.1
Gallus gallus (chicken)	CUL4B	XP_420335.2
Rattus norvegicus (Norway rat)	Cul4b	NP_001100421.1
Arabidopsis thaliana (thale cress)	CUL4	NP_568658.1
Xenopus tropicalis (tropical clawed frog)	cul4b	NP_001120891.1
Bos taurus (cattle)	CUL4B	XP_005227530.1
Drosophila melanogaster (fruit fly)	Cul-4	NP_001163084.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These studies provide insights into a novel role of cul-4 in promoting cell survival in the developing Drosophila eye.
Title: Cullin-4 regulates Wingless and JNK signaling-mediated cell death in the Drosophila eye.

BRWD3 functions as a Damage-specific DNA binding protein 1 (DDB1)- and CULLIN (CUL)4-associated factor in a Cullin4-RING Finger E3 Ligase (CRL4) that mediates light-dependent binding of dCRY to CUL4-ROC1-DDB1-BRWD3, inducing ubiquitylation of dCRY
Title: Ramshackle (Brwd3) promotes light-induced ubiquitylation of Drosophila Cryptochrome by DDB1-CUL4-ROC1 E3 ligase complex.

we could not detect Dup hyperaccumulation using mutations in the CRL4(Cdt2) components Cul4 and Ddb1, likely because these cause pleiotropic effects that block cell proliferation
Title: Cell type-dependent requirement for PIP box-regulated Cdt1 destruction during S phase.

Plays a conserved role in the regulation of beta-catenin levels.
Title: Cullin4B/E3-ubiquitin ligase negatively regulates beta-catenin.

loss of CUL4 in Drosophila cells causes G(1) cell cycle arrest and an increased protein level of the CDK inhibitor Dacapo
Title: Involvement of CUL4 ubiquitin E3 ligases in regulating CDK inhibitors Dacapo/p27Kip1 and cyclin E degradation.

The following Cul4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cul4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa09251	NM_136508.4 Latest version!	Drosophila melanogaster cullin 4, transcript variant A (Cul4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa09251	NM_001169613.1 Latest version!	Drosophila melanogaster cullin 4, transcript variant B (Cul4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cul4 ( NM_001169613.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163084.1 Drosophila melanogaster cullin 4, transcript variant B (Cul4), mRNA.

Price & Availability↑

CloneID	OFa09251
Clone ID Related Accession (Same CDS sequence)	NM_001169613.1 , NM_136508.4
Accession Version	NM_001169613.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cullin 4, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGCGG CCAAGAAGTA CAAGCCCATG GACACAACTG AGCTGCACGA GAACACAGAG 
AATATTGCCG CCTGCCTGAA GAAGGCCGAG AACGTCGCCC CCACCGCTGC TGCCGCCTCG 
AGCGTGGGCC GCAGTGCCTT CGCTGCCCAA AACATGAATT CTGCGTCCAC CAACGGCGAG 
CGCCTGCCAA ACTTCTCCAA GCTAGGCGGC AGCCATGGCG AGATCCGCAC GGCTTCAACT 
ACCTCAAATC TCCTCAATCG CATGGGGGCT ATTCACAACA GCAAGCCTGG CGATGTCAAG 
AAGATAGTGA TTAAGAACTT TAAGGATAAG CCTACACTGC CCGATAACTA TTCCAAGGAC 
ACGTACGTGA AGCTTGAAGA AGCCGTTATC GCCATCCAAC TCTCCAAGCC CATTAAATAT 
TCGCTGGAGG AGCTCTACCA GGCGGTGGTG AACATGTGTA GCCACAAGAT GGATGCACAA 
TTATACGCGA AACTCAAGGA GCTAACAGAG CAACACGTAA AGCGCAATAT TAAGCTCAAG 
GAGCTCACCG GCGGTAGCAT GGACAAACTG ATTTTGCTGG AAAAGATCAA CCACTGGTGG 
CTCTCGTTCT GCCAACAAAT GATCATGATT CGCAGCATAT TCTTGTATAT GGATCGAACT 
TATGTGCTGC AAAATTCGAC CGTTCACTCG ATTTGGGACA TGGGGCTGGA CCTTTTTCGC 
ATCCATTTTG CTCAAAACAG CGTTGTCCAA AAGCGAACCG TCGACGGTTT ACTAACGCTT 
ATTGAAAAAG AACGACAAGG CTCCACCGTG GACCGTGGTT TGCTGAAGAG CTTGGTTCGC 
ATGTTATGCG ACCTGCAGAT CTACACCAGC TCCTTTGAGG AGAAGTTCCT CGACGCAACC 
AATCAATTGT ATAAGGCTGA GAGTCAACGC AAGATGCAAG AACTGGAAGT TCCCGAGTAC 
TTACAACACG TCAACAAGCG TTTGGCCGAG GAGAACGAGC GTTTGCGTCA CTATCTGGAC 
TCGAGCACCA AACATCCACT GATTTACAAT GTGGAAAAAG AGTTACTTGC CGAACACCTA 
ACGTCAATAT TGCAGAAGGG CTTGGATTCA TTGTTGGAAG ACAACAGACT GAGTGACCTT 
ACACTGTTGT ACGGGCTGCT TAGTCGAGTC AAAAATGGAA CATCCGAGCT GTGCGGCAAT 
TTCAACGGTT TCATTAAGAA AAAGGGACGC ACCATTGTCA TCGATCCCGA GAAGGATAAG 
AGCATGGTCC AAGATTTGCT CGATTTCAAG GACAAAATGG ATGTGATAGT TCGCACCTGC 
TTTGAGCACA ATGAGAAATT CACAAACTCG TTGCGCGAGG CCTTTGAGTT CTTTATCAAC 
CAACGTGCCA ACAAGCCGGC CGAGCTTATT GCTAAATACG TAGATATGAA ACTGCGATCT 
GGCAACAAGG GAACCACAGA TGAGGAGTTG GAAAAGACTC TAGATAAAAT CATGGTTCTG 
TTTCGTTTTA TACATGGCAA AGATGTATTT GAAGCGTTTT ACAAAAAGGA CTTGGCCAAA 
CGATTACTTG TGGGCAAATC GGCATCAGTG GATTCCGAAA AGAGTATGCT CTCCAAGCTT 
AAGCAGGAGT GCGGTGGAGG GTTTACGTCC AAGCTGGAGG GCATGTTTAA GGACATGGAA 
CTGAGCCGAG ATATCAACAT TGCGTTCCGC GGACACGCGT TAAGCAACAA CCGCGATGTC 
CACAACTTGG ACTTGTGCGT CAGCATTCTT ACCATGGGCT ACTGGCCTAC ATATGCACCT 
ACAGAGGTCA CAATGCCGCC ACAGTTCATC AATCCTCAGC AGATTTTCAA CAAATTTTAT 
CTGGAAAAGC ATAGTGGGCG GAAGTTGCAG TGGCAGCCTA CATTAGGAAA TTGTATGCTG 
CGTGCTCAAT TCGATGCGGG ACCGAAAGAA TTGTTGGTCT CGCTGTTTCA AGCGCTTGTG 
CTTCTATTGT TTAACGATAA GCCAGTTCTG AGCTACGAGG AGATATTGGC CGCCACCCTC 
ATCGAGGATG GAGAGTTGCG TCGAACACTT CAGTCCCTGG CTTGTGGGCG CGCCCGCGTC 
ATAACAAAAA CACCGAAAGG CCGCGAAATC TTGGATGGCG ATCAGTTTGA TTTTAACAAT 
GAGTTCACAA ACAAATTGTT TAGAATCAAG ATCAACCAAA TACAGATGAA GGAGACAAAT 
GAGGAGCAAA AGGCGACGGA GGAGCGTGTG TTTCAGGATC GTCAGTATCA AATTGATGCG 
GCTATTGTGC GCATTATGAA AATGCGAAAA ACGCTGAGCC ATAATCTTCT CATCACCGAG 
CTGTTCAACC AGCTTACCTT CCCTGTCAAG CCCGCTGACC TAAAGAAGCG AATCGAGTCG 
CTCATCGACC GCGACTACAT GGAGCGAGAC AAGGATAATC AAAACCAATA CAACTATGTG 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163084.1
CDS	389..2854
Translation	MSAAKKYKPMDTTELHENTENIAACLKKAENVAPTAAAASSVGRSAFAAQNMNSASTNGERLPNFSKLGGSHGEIRTASTTSNLLNRMGAIHNSKPGDVKKIVIKNFKDKPTLPDNYSKDTYVKLEEAVIAIQLSKPIKYSLEELYQAVVNMCSHKMDAQLYAKLKELTEQHVKRNIKLKELTGGSMDKLILLEKINHWWLSFCQQMIMIRSIFLYMDRTYVLQNSTVHSIWDMGLDLFRIHFAQNSVVQKRTVDGLLTLIEKERQGSTVDRGLLKSLVRMLCDLQIYTSSFEEKFLDATNQLYKAESQRKMQELEVPEYLQHVNKRLAEENERLRHYLDSSTKHPLIYNVEKELLAEHLTSILQKGLDSLLEDNRLSDLTLLYGLLSRVKNGTSELCGNFNGFIKKKGRTIVIDPEKDKSMVQDLLDFKDKMDVIVRTCFEHNEKFTNSLREAFEFFINQRANKPAELIAKYVDMKLRSGNKGTTDEELEKTLDKIMVLFRFIHGKDVFEAFYKKDLAKRLLVGKSASVDSEKSMLSKLKQECGGGFTSKLEGMFKDMELSRDINIAFRGHALSNNRDVHNLDLCVSILTMGYWPTYAPTEVTMPPQFINPQQIFNKFYLEKHSGRKLQWQPTLGNCMLRAQFDAGPKELLVSLFQALVLLLFNDKPVLSYEEILAATLIEDGELRRTLQSLACGRARVITKTPKGREILDGDQFDFNNEFTNKLFRIKINQIQMKETNEEQKATEERVFQDRQYQIDAAIVRIMKMRKTLSHNLLITELFNQLTFPVKPADLKKRIESLIDRDYMERDKDNQNQYNYVA

Target ORF information:

RefSeq Version	NM_001169613.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cullin 4, transcript variant B (Cul4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169613.1

ORF Insert Sequence:

ATGAGTGCGG CCAAGAAGTA CAAGCCCATG GACACAACTG AGCTGCACGA GAACACAGAG 
AATATTGCCG CCTGCCTGAA GAAGGCCGAG AACGTCGCCC CCACCGCTGC TGCCGCCTCG 
AGCGTGGGCC GCAGTGCCTT CGCTGCCCAA AACATGAATT CTGCGTCCAC CAACGGCGAG 
CGCCTGCCAA ACTTCTCCAA GCTAGGCGGC AGCCATGGCG AGATCCGCAC GGCTTCAACT 
ACCTCAAATC TCCTCAATCG CATGGGGGCT ATTCACAACA GCAAGCCTGG CGATGTCAAG 
AAGATAGTGA TTAAGAACTT TAAGGATAAG CCTACACTGC CCGATAACTA TTCCAAGGAC 
ACGTACGTGA AGCTTGAAGA AGCCGTTATC GCCATCCAAC TCTCCAAGCC CATTAAATAT 
TCGCTGGAGG AGCTCTACCA GGCGGTGGTG AACATGTGTA GCCACAAGAT GGATGCACAA 
TTATACGCGA AACTCAAGGA GCTAACAGAG CAACACGTAA AGCGCAATAT TAAGCTCAAG 
GAGCTCACCG GCGGTAGCAT GGACAAACTG ATTTTGCTGG AAAAGATCAA CCACTGGTGG 
CTCTCGTTCT GCCAACAAAT GATCATGATT CGCAGCATAT TCTTGTATAT GGATCGAACT 
TATGTGCTGC AAAATTCGAC CGTTCACTCG ATTTGGGACA TGGGGCTGGA CCTTTTTCGC 
ATCCATTTTG CTCAAAACAG CGTTGTCCAA AAGCGAACCG TCGACGGTTT ACTAACGCTT 
ATTGAAAAAG AACGACAAGG CTCCACCGTG GACCGTGGTT TGCTGAAGAG CTTGGTTCGC 
ATGTTATGCG ACCTGCAGAT CTACACCAGC TCCTTTGAGG AGAAGTTCCT CGACGCAACC 
AATCAATTGT ATAAGGCTGA GAGTCAACGC AAGATGCAAG AACTGGAAGT TCCCGAGTAC 
TTACAACACG TCAACAAGCG TTTGGCCGAG GAGAACGAGC GTTTGCGTCA CTATCTGGAC 
TCGAGCACCA AACATCCACT GATTTACAAT GTGGAAAAAG AGTTACTTGC CGAACACCTA 
ACGTCAATAT TGCAGAAGGG CTTGGATTCA TTGTTGGAAG ACAACAGACT GAGTGACCTT 
ACACTGTTGT ACGGGCTGCT TAGTCGAGTC AAAAATGGAA CATCCGAGCT GTGCGGCAAT 
TTCAACGGTT TCATTAAGAA AAAGGGACGC ACCATTGTCA TCGATCCCGA GAAGGATAAG 
AGCATGGTCC AAGATTTGCT CGATTTCAAG GACAAAATGG ATGTGATAGT TCGCACCTGC 
TTTGAGCACA ATGAGAAATT CACAAACTCG TTGCGCGAGG CCTTTGAGTT CTTTATCAAC 
CAACGTGCCA ACAAGCCGGC CGAGCTTATT GCTAAATACG TAGATATGAA ACTGCGATCT 
GGCAACAAGG GAACCACAGA TGAGGAGTTG GAAAAGACTC TAGATAAAAT CATGGTTCTG 
TTTCGTTTTA TACATGGCAA AGATGTATTT GAAGCGTTTT ACAAAAAGGA CTTGGCCAAA 
CGATTACTTG TGGGCAAATC GGCATCAGTG GATTCCGAAA AGAGTATGCT CTCCAAGCTT 
AAGCAGGAGT GCGGTGGAGG GTTTACGTCC AAGCTGGAGG GCATGTTTAA GGACATGGAA 
CTGAGCCGAG ATATCAACAT TGCGTTCCGC GGACACGCGT TAAGCAACAA CCGCGATGTC 
CACAACTTGG ACTTGTGCGT CAGCATTCTT ACCATGGGCT ACTGGCCTAC ATATGCACCT 
ACAGAGGTCA CAATGCCGCC ACAGTTCATC AATCCTCAGC AGATTTTCAA CAAATTTTAT 
CTGGAAAAGC ATAGTGGGCG GAAGTTGCAG TGGCAGCCTA CATTAGGAAA TTGTATGCTG 
CGTGCTCAAT TCGATGCGGG ACCGAAAGAA TTGTTGGTCT CGCTGTTTCA AGCGCTTGTG 
CTTCTATTGT TTAACGATAA GCCAGTTCTG AGCTACGAGG AGATATTGGC CGCCACCCTC 
ATCGAGGATG GAGAGTTGCG TCGAACACTT CAGTCCCTGG CTTGTGGGCG CGCCCGCGTC 
ATAACAAAAA CACCGAAAGG CCGCGAAATC TTGGATGGCG ATCAGTTTGA TTTTAACAAT 
GAGTTCACAA ACAAATTGTT TAGAATCAAG ATCAACCAAA TACAGATGAA GGAGACAAAT 
GAGGAGCAAA AGGCGACGGA GGAGCGTGTG TTTCAGGATC GTCAGTATCA AATTGATGCG 
GCTATTGTGC GCATTATGAA AATGCGAAAA ACGCTGAGCC ATAATCTTCT CATCACCGAG 
CTGTTCAACC AGCTTACCTT CCCTGTCAAG CCCGCTGACC TAAAGAAGCG AATCGAGTCG 
CTCATCGACC GCGACTACAT GGAGCGAGAC AAGGATAATC AAAACCAATA CAACTATGTG 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cul4 ( NM_136508.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610352.2 Drosophila melanogaster cullin 4, transcript variant A (Cul4), mRNA.

Price & Availability↑

CloneID	OFa09251
Clone ID Related Accession (Same CDS sequence)	NM_001169613.1 , NM_136508.4
Accession Version	NM_136508.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cullin 4, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_136508.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGCGG CCAAGAAGTA CAAGCCCATG GACACAACTG AGCTGCACGA GAACACAGAG 
AATATTGCCG CCTGCCTGAA GAAGGCCGAG AACGTCGCCC CCACCGCTGC TGCCGCCTCG 
AGCGTGGGCC GCAGTGCCTT CGCTGCCCAA AACATGAATT CTGCGTCCAC CAACGGCGAG 
CGCCTGCCAA ACTTCTCCAA GCTAGGCGGC AGCCATGGCG AGATCCGCAC GGCTTCAACT 
ACCTCAAATC TCCTCAATCG CATGGGGGCT ATTCACAACA GCAAGCCTGG CGATGTCAAG 
AAGATAGTGA TTAAGAACTT TAAGGATAAG CCTACACTGC CCGATAACTA TTCCAAGGAC 
ACGTACGTGA AGCTTGAAGA AGCCGTTATC GCCATCCAAC TCTCCAAGCC CATTAAATAT 
TCGCTGGAGG AGCTCTACCA GGCGGTGGTG AACATGTGTA GCCACAAGAT GGATGCACAA 
TTATACGCGA AACTCAAGGA GCTAACAGAG CAACACGTAA AGCGCAATAT TAAGCTCAAG 
GAGCTCACCG GCGGTAGCAT GGACAAACTG ATTTTGCTGG AAAAGATCAA CCACTGGTGG 
CTCTCGTTCT GCCAACAAAT GATCATGATT CGCAGCATAT TCTTGTATAT GGATCGAACT 
TATGTGCTGC AAAATTCGAC CGTTCACTCG ATTTGGGACA TGGGGCTGGA CCTTTTTCGC 
ATCCATTTTG CTCAAAACAG CGTTGTCCAA AAGCGAACCG TCGACGGTTT ACTAACGCTT 
ATTGAAAAAG AACGACAAGG CTCCACCGTG GACCGTGGTT TGCTGAAGAG CTTGGTTCGC 
ATGTTATGCG ACCTGCAGAT CTACACCAGC TCCTTTGAGG AGAAGTTCCT CGACGCAACC 
AATCAATTGT ATAAGGCTGA GAGTCAACGC AAGATGCAAG AACTGGAAGT TCCCGAGTAC 
TTACAACACG TCAACAAGCG TTTGGCCGAG GAGAACGAGC GTTTGCGTCA CTATCTGGAC 
TCGAGCACCA AACATCCACT GATTTACAAT GTGGAAAAAG AGTTACTTGC CGAACACCTA 
ACGTCAATAT TGCAGAAGGG CTTGGATTCA TTGTTGGAAG ACAACAGACT GAGTGACCTT 
ACACTGTTGT ACGGGCTGCT TAGTCGAGTC AAAAATGGAA CATCCGAGCT GTGCGGCAAT 
TTCAACGGTT TCATTAAGAA AAAGGGACGC ACCATTGTCA TCGATCCCGA GAAGGATAAG 
AGCATGGTCC AAGATTTGCT CGATTTCAAG GACAAAATGG ATGTGATAGT TCGCACCTGC 
TTTGAGCACA ATGAGAAATT CACAAACTCG TTGCGCGAGG CCTTTGAGTT CTTTATCAAC 
CAACGTGCCA ACAAGCCGGC CGAGCTTATT GCTAAATACG TAGATATGAA ACTGCGATCT 
GGCAACAAGG GAACCACAGA TGAGGAGTTG GAAAAGACTC TAGATAAAAT CATGGTTCTG 
TTTCGTTTTA TACATGGCAA AGATGTATTT GAAGCGTTTT ACAAAAAGGA CTTGGCCAAA 
CGATTACTTG TGGGCAAATC GGCATCAGTG GATTCCGAAA AGAGTATGCT CTCCAAGCTT 
AAGCAGGAGT GCGGTGGAGG GTTTACGTCC AAGCTGGAGG GCATGTTTAA GGACATGGAA 
CTGAGCCGAG ATATCAACAT TGCGTTCCGC GGACACGCGT TAAGCAACAA CCGCGATGTC 
CACAACTTGG ACTTGTGCGT CAGCATTCTT ACCATGGGCT ACTGGCCTAC ATATGCACCT 
ACAGAGGTCA CAATGCCGCC ACAGTTCATC AATCCTCAGC AGATTTTCAA CAAATTTTAT 
CTGGAAAAGC ATAGTGGGCG GAAGTTGCAG TGGCAGCCTA CATTAGGAAA TTGTATGCTG 
CGTGCTCAAT TCGATGCGGG ACCGAAAGAA TTGTTGGTCT CGCTGTTTCA AGCGCTTGTG 
CTTCTATTGT TTAACGATAA GCCAGTTCTG AGCTACGAGG AGATATTGGC CGCCACCCTC 
ATCGAGGATG GAGAGTTGCG TCGAACACTT CAGTCCCTGG CTTGTGGGCG CGCCCGCGTC 
ATAACAAAAA CACCGAAAGG CCGCGAAATC TTGGATGGCG ATCAGTTTGA TTTTAACAAT 
GAGTTCACAA ACAAATTGTT TAGAATCAAG ATCAACCAAA TACAGATGAA GGAGACAAAT 
GAGGAGCAAA AGGCGACGGA GGAGCGTGTG TTTCAGGATC GTCAGTATCA AATTGATGCG 
GCTATTGTGC GCATTATGAA AATGCGAAAA ACGCTGAGCC ATAATCTTCT CATCACCGAG 
CTGTTCAACC AGCTTACCTT CCCTGTCAAG CCCGCTGACC TAAAGAAGCG AATCGAGTCG 
CTCATCGACC GCGACTACAT GGAGCGAGAC AAGGATAATC AAAACCAATA CAACTATGTG 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610352.2
CDS	393..2858
Translation	MSAAKKYKPMDTTELHENTENIAACLKKAENVAPTAAAASSVGRSAFAAQNMNSASTNGERLPNFSKLGGSHGEIRTASTTSNLLNRMGAIHNSKPGDVKKIVIKNFKDKPTLPDNYSKDTYVKLEEAVIAIQLSKPIKYSLEELYQAVVNMCSHKMDAQLYAKLKELTEQHVKRNIKLKELTGGSMDKLILLEKINHWWLSFCQQMIMIRSIFLYMDRTYVLQNSTVHSIWDMGLDLFRIHFAQNSVVQKRTVDGLLTLIEKERQGSTVDRGLLKSLVRMLCDLQIYTSSFEEKFLDATNQLYKAESQRKMQELEVPEYLQHVNKRLAEENERLRHYLDSSTKHPLIYNVEKELLAEHLTSILQKGLDSLLEDNRLSDLTLLYGLLSRVKNGTSELCGNFNGFIKKKGRTIVIDPEKDKSMVQDLLDFKDKMDVIVRTCFEHNEKFTNSLREAFEFFINQRANKPAELIAKYVDMKLRSGNKGTTDEELEKTLDKIMVLFRFIHGKDVFEAFYKKDLAKRLLVGKSASVDSEKSMLSKLKQECGGGFTSKLEGMFKDMELSRDINIAFRGHALSNNRDVHNLDLCVSILTMGYWPTYAPTEVTMPPQFINPQQIFNKFYLEKHSGRKLQWQPTLGNCMLRAQFDAGPKELLVSLFQALVLLLFNDKPVLSYEEILAATLIEDGELRRTLQSLACGRARVITKTPKGREILDGDQFDFNNEFTNKLFRIKINQIQMKETNEEQKATEERVFQDRQYQIDAAIVRIMKMRKTLSHNLLITELFNQLTFPVKPADLKKRIESLIDRDYMERDKDNQNQYNYVA

Target ORF information:

RefSeq Version	NM_136508.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cullin 4, transcript variant A (Cul4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136508.4

ORF Insert Sequence:

ATGAGTGCGG CCAAGAAGTA CAAGCCCATG GACACAACTG AGCTGCACGA GAACACAGAG 
AATATTGCCG CCTGCCTGAA GAAGGCCGAG AACGTCGCCC CCACCGCTGC TGCCGCCTCG 
AGCGTGGGCC GCAGTGCCTT CGCTGCCCAA AACATGAATT CTGCGTCCAC CAACGGCGAG 
CGCCTGCCAA ACTTCTCCAA GCTAGGCGGC AGCCATGGCG AGATCCGCAC GGCTTCAACT 
ACCTCAAATC TCCTCAATCG CATGGGGGCT ATTCACAACA GCAAGCCTGG CGATGTCAAG 
AAGATAGTGA TTAAGAACTT TAAGGATAAG CCTACACTGC CCGATAACTA TTCCAAGGAC 
ACGTACGTGA AGCTTGAAGA AGCCGTTATC GCCATCCAAC TCTCCAAGCC CATTAAATAT 
TCGCTGGAGG AGCTCTACCA GGCGGTGGTG AACATGTGTA GCCACAAGAT GGATGCACAA 
TTATACGCGA AACTCAAGGA GCTAACAGAG CAACACGTAA AGCGCAATAT TAAGCTCAAG 
GAGCTCACCG GCGGTAGCAT GGACAAACTG ATTTTGCTGG AAAAGATCAA CCACTGGTGG 
CTCTCGTTCT GCCAACAAAT GATCATGATT CGCAGCATAT TCTTGTATAT GGATCGAACT 
TATGTGCTGC AAAATTCGAC CGTTCACTCG ATTTGGGACA TGGGGCTGGA CCTTTTTCGC 
ATCCATTTTG CTCAAAACAG CGTTGTCCAA AAGCGAACCG TCGACGGTTT ACTAACGCTT 
ATTGAAAAAG AACGACAAGG CTCCACCGTG GACCGTGGTT TGCTGAAGAG CTTGGTTCGC 
ATGTTATGCG ACCTGCAGAT CTACACCAGC TCCTTTGAGG AGAAGTTCCT CGACGCAACC 
AATCAATTGT ATAAGGCTGA GAGTCAACGC AAGATGCAAG AACTGGAAGT TCCCGAGTAC 
TTACAACACG TCAACAAGCG TTTGGCCGAG GAGAACGAGC GTTTGCGTCA CTATCTGGAC 
TCGAGCACCA AACATCCACT GATTTACAAT GTGGAAAAAG AGTTACTTGC CGAACACCTA 
ACGTCAATAT TGCAGAAGGG CTTGGATTCA TTGTTGGAAG ACAACAGACT GAGTGACCTT 
ACACTGTTGT ACGGGCTGCT TAGTCGAGTC AAAAATGGAA CATCCGAGCT GTGCGGCAAT 
TTCAACGGTT TCATTAAGAA AAAGGGACGC ACCATTGTCA TCGATCCCGA GAAGGATAAG 
AGCATGGTCC AAGATTTGCT CGATTTCAAG GACAAAATGG ATGTGATAGT TCGCACCTGC 
TTTGAGCACA ATGAGAAATT CACAAACTCG TTGCGCGAGG CCTTTGAGTT CTTTATCAAC 
CAACGTGCCA ACAAGCCGGC CGAGCTTATT GCTAAATACG TAGATATGAA ACTGCGATCT 
GGCAACAAGG GAACCACAGA TGAGGAGTTG GAAAAGACTC TAGATAAAAT CATGGTTCTG 
TTTCGTTTTA TACATGGCAA AGATGTATTT GAAGCGTTTT ACAAAAAGGA CTTGGCCAAA 
CGATTACTTG TGGGCAAATC GGCATCAGTG GATTCCGAAA AGAGTATGCT CTCCAAGCTT 
AAGCAGGAGT GCGGTGGAGG GTTTACGTCC AAGCTGGAGG GCATGTTTAA GGACATGGAA 
CTGAGCCGAG ATATCAACAT TGCGTTCCGC GGACACGCGT TAAGCAACAA CCGCGATGTC 
CACAACTTGG ACTTGTGCGT CAGCATTCTT ACCATGGGCT ACTGGCCTAC ATATGCACCT 
ACAGAGGTCA CAATGCCGCC ACAGTTCATC AATCCTCAGC AGATTTTCAA CAAATTTTAT 
CTGGAAAAGC ATAGTGGGCG GAAGTTGCAG TGGCAGCCTA CATTAGGAAA TTGTATGCTG 
CGTGCTCAAT TCGATGCGGG ACCGAAAGAA TTGTTGGTCT CGCTGTTTCA AGCGCTTGTG 
CTTCTATTGT TTAACGATAA GCCAGTTCTG AGCTACGAGG AGATATTGGC CGCCACCCTC 
ATCGAGGATG GAGAGTTGCG TCGAACACTT CAGTCCCTGG CTTGTGGGCG CGCCCGCGTC 
ATAACAAAAA CACCGAAAGG CCGCGAAATC TTGGATGGCG ATCAGTTTGA TTTTAACAAT 
GAGTTCACAA ACAAATTGTT TAGAATCAAG ATCAACCAAA TACAGATGAA GGAGACAAAT 
GAGGAGCAAA AGGCGACGGA GGAGCGTGTG TTTCAGGATC GTCAGTATCA AATTGATGCG 
GCTATTGTGC GCATTATGAA AATGCGAAAA ACGCTGAGCC ATAATCTTCT CATCACCGAG 
CTGTTCAACC AGCTTACCTT CCCTGTCAAG CCCGCTGACC TAAAGAAGCG AATCGAGTCG 
CTCATCGACC GCGACTACAT GGAGCGAGAC AAGGATAATC AAAACCAATA CAACTATGTG 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Cul4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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