cul4b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	cul4b
Entrez Gene ID	560313
Full Name	cullin 4B
Synonyms	im:7140950,im:7150765
General protein information	Preferred Names cullin 4B Names cullin-4B
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001122844.1	NP_001116316.1	cullin-4B

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CUL4B	XP_864628.3
Mus musculus (house mouse)	Cul4b	NP_001103612.1
Danio rerio (zebrafish)	cul4b	NP_001116316.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007727	XP_308149.4
Homo sapiens (human)	CUL4B	NP_001073341.1
	CUL4B	XP_001086195.1
Gallus gallus (chicken)	CUL4B	XP_420335.2
Rattus norvegicus (Norway rat)	Cul4b	NP_001100421.1
Arabidopsis thaliana (thale cress)	CUL4	NP_568658.1
Xenopus tropicalis (tropical clawed frog)	cul4b	NP_001120891.1
Bos taurus (cattle)	CUL4B	XP_005227530.1
Drosophila melanogaster (fruit fly)	Cul-4	NP_001163084.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Zebrafish cul4a, but not cul4b, modulates cardiac and forelimb development by upregulating tbx5a expression.
Zhao X, Jiang B, Hu H, Mao F, Mi J, Li Z, Liu Q, Shao C, Gong Y
Human molecular genetics24(3)853-64(2015 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results show that cul4a but not cul4b is required for the expression of tbx5a, an essential transcription factor in heart and limb development.
Title: Zebrafish cul4a, but not cul4b, modulates cardiac and forelimb development by upregulating tbx5a expression.

The following cul4b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cul4b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa32207	NM_001122844.1 Latest version!	Danio rerio cullin 4B (cul4b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cul4b ( NM_001122844.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001116316.1 Danio rerio cullin 4B (cul4b), mRNA.

Price & Availability↑

CloneID	ODa32207
Clone ID Related Accession (Same CDS sequence)	NM_001122844.1
Accession Version	NM_001122844.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2595bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-14
Organism	Danio rerio(zebrafish)
Product	cullin-4B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX004980.8. On Mar 27, 2008 this sequence version replaced XM_680523.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GDQH01010799.1, GDQQ01014232.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTTTCCAA CAGGTTTACC TTCCCCTAAT CCCCCACCGC CAGCCCAAGA GCCCAGACCG 
GCAGCTTCTG ATGTTCACAA CGACAGTAGC TTAACGTCTT CTAAGAAGAG GAAAATCAAC 
AGTTCAGACA AGGAAGACAT TGATTCAATA TCATCGTCTC CTAAAGCCAT CTGCAATTCC 
TCCTCCACAA CGTCCTGCTC TTCCAGTTCA CAACAACAGC AGCAACAACA GCACATCCAG 
AAGAAGTTGA GGTTCGAGGA CCCGCTGGAT TTGATCGGAC TGGATGTGAA GATGGCCGAG 
GAGTCCTGTA ACCCATCCGA ATCCTGTTCA AAGGCGAGAA ATGTGTTTCT GCCCGGCGGT 
GTTGGGCATC ATGCTAACGG ACTGACCAAG TCTGCGGGCT CCGCTACCTT CTCCAACAGT 
AAACCCGGTG CAGCGAAGAA ACTTGTTATC AAGAACTTTA AAGAAAAGCC CAAATTACCA 
GAGAATTACA CAAATGAGAC CTGGCAGAAA CTAAAGGAGG CAGTAGAGGC CATACAGAAC 
AGCACTTCAA TAAAGTACAA TCTAGAGGAG CTCTATCAGG CTGTTGAAAA CTTGTGCTCT 
CATAAGATAT CTGCCAAGCT GTATAAACAG CTCAGAGTGG TTTGTGAAGA CCACATCAAG 
GCACAGATTG ACCAGTTCAG AGAGGATGCC CTAGACAGTG TTCTGTTCCT CAAGAAAATA 
GACAAATGTT GGCAAGATCA CTGCAGACAA ATGATCATGA TTAGGAGTAT ATTTTTGTTT 
CTGGATCGCA CATATGTTTT ACAAAATTCT ATGCTGCCAT CAATCTGGGA CATGGGCTTG 
GAGTTGTTTC GTTTCTATAT CATCAGCGAC CTGAAGGTGC AGAGTAAGAC AATCGATGGG 
ATTCTGCTTC TTATTGAGAG GGAGCGAAGC GGCGAGGCTG TAGACCGCAG TCTTCTGAGG 
AGTCTTCTGA GCATGCTCTC TGACCTGCAG ATTTATCAGG ATTCATTTGA GCAGCGCTTT 
CTGGAGGAGA CAAATCGACT GTATGCTGCT GAGGGCCAGA GGCTCATGCA GGAAAGAGAG 
GTGCCAGAGT ATCTCCATCA TGTCAACAAA CGTCTAGAAG AAGAGGCAGA CAGAGTCATC 
ACATACTTAG ACCAGAGCAC ACAAAAATCC CTCATTGCCA CAGTGGAAAA GCAGCTGCTT 
GGAGAACACC TCACTGCAAT ACTTCAGAAA GGTTTGAATA ACTTGCTGGA TGAGAACCGT 
ATTCAGGATC TGTCTCTGCT GTATCAGCTC TTCAGTCGCG TGAGAGGTGG TGTGCAGGTT 
CTTCTGCAGC ATTGGATCGA GTACATCAAG GCCTTTGGAA GCACAATTGT GATCAACCCT 
GAGAAAGACA AGACGATGGT TCAGGAGCTT CTGGATTTCA AGGATAAAGT CGACCACATA 
ATTGATGTAT GTTTCATGAA GAATGAAAAG TTTGTAAATG GCATGAAGGA AGCCTTCGAG 
ACGTTTATCA ACAAACGGCC AAACAAACCT GCAGAGCTCA TAGCAAAATA TGTGGATTCA 
AAGCTCCGTG CTGGGAACAA AGAGGCAACT GATGAGGAGC TGGAGAAAAT GTTGGACAAG 
ATAATGATCA TATTCAGGTT CATTTACGGC AAAGATGTTT TTGAAGCCTT CTACAAGAAA 
GACTTAGCGA AGAGGTTACT GGTGGGAAAG AGTGCCTCTG TAGATGCAGA GAAATCAATG 
CTGTCAAAAC TGAAGCATGA ATGTGGAGCA GCATTTACTA GTAAACTGGA GGGAATGTTC 
AAAGACATGG AGCTGTCGAA AGATATTATG GTGCAGTTCA AACAGCATAT GCAGTGCCAA 
AATATTCCTG GTAACATTGA GCTGACAGTG AACATCCTCA CTATGGGCTA TTGGCCAACA 
TACGTCCCTA TGGAAGTACA TCTGCCAGCA GAGATGGTGA GATTGCAAGA GATCTTCAAA 
ACGTTTTACC TGGGTAAACA CAGTGGACGG AAACTACAGT GGCAATCAAC GTTAGGGCAT 
TGTGTTTTAA AGGCAGAATT TAAAGAGGGG AAAAAGGAGC TGCAGGTGTC TCTGTTTCAG 
ACTTTAGTTC TGCTTATGTT TAATGAAGGG GAGGAATTCT CTCTGGAGGA CATTAAGCTG 
GCCACAGGCA TTGAGGACAG TGAGCTGAGG CGGACACTGC AGTCTCTGGC CTGCGGGAAA 
GCAAGAGTCC TCACCAAAAT CCCAAAGAGT AAAGATGTGG AGGATGGAGA CAAGTTCTCA 
TGCAACGATG ATTTCAAACA CAAACTCTTC AGAATTAAAA TCAACCAGAT CCAGATGAAA 
GAAACGGTGG AGGAGCAAGC CAGCACTACA GAGAGAGTAT TCCAGGATCG TCAGTACCAA 
ATCGATGCTG CCATTGTTCG AATCATGAAG ATGAGGAAAA CTCTCAGCCA TAATCTTCTG 
GTGTCTGAGG TGTACAACCA ACTTAAGTTC CCAGTCAAGC CTGCTGATCT GAAGAAGAGG 
ATAGAGTCAC TTATTGACAG GGACTACATG GAAAGGGATA AAGAGAATCC CAATCAATAC 
AACTATGTGG CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001116316.1
CDS	441..3035
Translation	MFPTGLPSPNPPPPAQEPRPAASDVHNDSSLTSSKKRKINSSDKEDIDSISSSPKAICNSSSTTSCSSSSQQQQQQQHIQKKLRFEDPLDLIGLDVKMAEESCNPSESCSKARNVFLPGGVGHHANGLTKSAGSATFSNSKPGAAKKLVIKNFKEKPKLPENYTNETWQKLKEAVEAIQNSTSIKYNLEELYQAVENLCSHKISAKLYKQLRVVCEDHIKAQIDQFREDALDSVLFLKKIDKCWQDHCRQMIMIRSIFLFLDRTYVLQNSMLPSIWDMGLELFRFYIISDLKVQSKTIDGILLLIERERSGEAVDRSLLRSLLSMLSDLQIYQDSFEQRFLEETNRLYAAEGQRLMQEREVPEYLHHVNKRLEEEADRVITYLDQSTQKSLIATVEKQLLGEHLTAILQKGLNNLLDENRIQDLSLLYQLFSRVRGGVQVLLQHWIEYIKAFGSTIVINPEKDKTMVQELLDFKDKVDHIIDVCFMKNEKFVNGMKEAFETFINKRPNKPAELIAKYVDSKLRAGNKEATDEELEKMLDKIMIIFRFIYGKDVFEAFYKKDLAKRLLVGKSASVDAEKSMLSKLKHECGAAFTSKLEGMFKDMELSKDIMVQFKQHMQCQNIPGNIELTVNILTMGYWPTYVPMEVHLPAEMVRLQEIFKTFYLGKHSGRKLQWQSTLGHCVLKAEFKEGKKELQVSLFQTLVLLMFNEGEEFSLEDIKLATGIEDSELRRTLQSLACGKARVLTKIPKSKDVEDGDKFSCNDDFKHKLFRIKINQIQMKETVEEQASTTERVFQDRQYQIDAAIVRIMKMRKTLSHNLLVSEVYNQLKFPVKPADLKKRIESLIDRDYMERDKENPNQYNYVA

Target ORF information:

RefSeq Version	NM_001122844.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cullin 4B (cul4b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001122844.1

ORF Insert Sequence:

ATGTTTCCAA CAGGTTTACC TTCCCCTAAT CCCCCACCGC CAGCCCAAGA GCCCAGACCG 
GCAGCTTCTG ATGTTCACAA CGACAGTAGC TTAACGTCTT CTAAGAAGAG GAAAATCAAC 
AGTTCAGACA AGGAAGACAT TGATTCAATA TCATCGTCTC CTAAAGCCAT CTGCAATTCC 
TCCTCCACAA CGTCCTGCTC TTCCAGTTCA CAACAACAGC AGCAACAACA GCACATCCAG 
AAGAAGTTGA GGTTCGAGGA CCCGCTGGAT TTGATCGGAC TGGATGTGAA GATGGCCGAG 
GAGTCCTGTA ACCCATCCGA ATCCTGTTCA AAGGCGAGAA ATGTGTTTCT GCCCGGCGGT 
GTTGGGCATC ATGCTAACGG ACTGACCAAG TCTGCGGGCT CCGCTACCTT CTCCAACAGT 
AAACCCGGTG CAGCGAAGAA ACTTGTTATC AAGAACTTTA AAGAAAAGCC CAAATTACCA 
GAGAATTACA CAAATGAGAC CTGGCAGAAA CTAAAGGAGG CAGTAGAGGC CATACAGAAC 
AGCACTTCAA TAAAGTACAA TCTAGAGGAG CTCTATCAGG CTGTTGAAAA CTTGTGCTCT 
CATAAGATAT CTGCCAAGCT GTATAAACAG CTCAGAGTGG TTTGTGAAGA CCACATCAAG 
GCACAGATTG ACCAGTTCAG AGAGGATGCC CTAGACAGTG TTCTGTTCCT CAAGAAAATA 
GACAAATGTT GGCAAGATCA CTGCAGACAA ATGATCATGA TTAGGAGTAT ATTTTTGTTT 
CTGGATCGCA CATATGTTTT ACAAAATTCT ATGCTGCCAT CAATCTGGGA CATGGGCTTG 
GAGTTGTTTC GTTTCTATAT CATCAGCGAC CTGAAGGTGC AGAGTAAGAC AATCGATGGG 
ATTCTGCTTC TTATTGAGAG GGAGCGAAGC GGCGAGGCTG TAGACCGCAG TCTTCTGAGG 
AGTCTTCTGA GCATGCTCTC TGACCTGCAG ATTTATCAGG ATTCATTTGA GCAGCGCTTT 
CTGGAGGAGA CAAATCGACT GTATGCTGCT GAGGGCCAGA GGCTCATGCA GGAAAGAGAG 
GTGCCAGAGT ATCTCCATCA TGTCAACAAA CGTCTAGAAG AAGAGGCAGA CAGAGTCATC 
ACATACTTAG ACCAGAGCAC ACAAAAATCC CTCATTGCCA CAGTGGAAAA GCAGCTGCTT 
GGAGAACACC TCACTGCAAT ACTTCAGAAA GGTTTGAATA ACTTGCTGGA TGAGAACCGT 
ATTCAGGATC TGTCTCTGCT GTATCAGCTC TTCAGTCGCG TGAGAGGTGG TGTGCAGGTT 
CTTCTGCAGC ATTGGATCGA GTACATCAAG GCCTTTGGAA GCACAATTGT GATCAACCCT 
GAGAAAGACA AGACGATGGT TCAGGAGCTT CTGGATTTCA AGGATAAAGT CGACCACATA 
ATTGATGTAT GTTTCATGAA GAATGAAAAG TTTGTAAATG GCATGAAGGA AGCCTTCGAG 
ACGTTTATCA ACAAACGGCC AAACAAACCT GCAGAGCTCA TAGCAAAATA TGTGGATTCA 
AAGCTCCGTG CTGGGAACAA AGAGGCAACT GATGAGGAGC TGGAGAAAAT GTTGGACAAG 
ATAATGATCA TATTCAGGTT CATTTACGGC AAAGATGTTT TTGAAGCCTT CTACAAGAAA 
GACTTAGCGA AGAGGTTACT GGTGGGAAAG AGTGCCTCTG TAGATGCAGA GAAATCAATG 
CTGTCAAAAC TGAAGCATGA ATGTGGAGCA GCATTTACTA GTAAACTGGA GGGAATGTTC 
AAAGACATGG AGCTGTCGAA AGATATTATG GTGCAGTTCA AACAGCATAT GCAGTGCCAA 
AATATTCCTG GTAACATTGA GCTGACAGTG AACATCCTCA CTATGGGCTA TTGGCCAACA 
TACGTCCCTA TGGAAGTACA TCTGCCAGCA GAGATGGTGA GATTGCAAGA GATCTTCAAA 
ACGTTTTACC TGGGTAAACA CAGTGGACGG AAACTACAGT GGCAATCAAC GTTAGGGCAT 
TGTGTTTTAA AGGCAGAATT TAAAGAGGGG AAAAAGGAGC TGCAGGTGTC TCTGTTTCAG 
ACTTTAGTTC TGCTTATGTT TAATGAAGGG GAGGAATTCT CTCTGGAGGA CATTAAGCTG 
GCCACAGGCA TTGAGGACAG TGAGCTGAGG CGGACACTGC AGTCTCTGGC CTGCGGGAAA 
GCAAGAGTCC TCACCAAAAT CCCAAAGAGT AAAGATGTGG AGGATGGAGA CAAGTTCTCA 
TGCAACGATG ATTTCAAACA CAAACTCTTC AGAATTAAAA TCAACCAGAT CCAGATGAAA 
GAAACGGTGG AGGAGCAAGC CAGCACTACA GAGAGAGTAT TCCAGGATCG TCAGTACCAA 
ATCGATGCTG CCATTGTTCG AATCATGAAG ATGAGGAAAA CTCTCAGCCA TAATCTTCTG 
GTGTCTGAGG TGTACAACCA ACTTAAGTTC CCAGTCAAGC CTGCTGATCT GAAGAAGAGG 
ATAGAGTCAC TTATTGACAG GGACTACATG GAAAGGGATA AAGAGAATCC CAATCAATAC 
AACTATGTGG CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Zebrafish cul4a, but not cul4b, modulates cardiac and forelimb development by upregulating tbx5a expression.
Human molecular genetics24(3)853-64(2015 Feb)
Zhao X,Jiang B,Hu H,Mao F,Mi J,Li Z,Liu Q,Shao C,Gong Y

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cul4b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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