clumsy cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	clumsy
Entrez Gene ID	35394
Full Name	CG8681 gene product from transcript CG8681-RB
Synonyms	CG8681,CT9037,Clumsy,Dmel\CG8681,Dmelclumsy,GluR39B
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 39B2-39B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001042908.2	NP_001036373.1	clumsy, isoform B
NM_078886.3	NP_523610.2	clumsy, isoform A

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000803	XP_311343.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012447	XP_309276.4
Drosophila melanogaster (fruit fly)	clumsy	NP_001036373.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following clumsy gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the clumsy cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa15111	NM_078886.3 Latest version!	Drosophila melanogaster clumsy, transcript variant A (clumsy), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa15110	NM_001042908.2 Latest version!	Drosophila melanogaster clumsy, transcript variant B (clumsy), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

clumsy ( NM_078886.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523610.2 Drosophila melanogaster clumsy, transcript variant A (clumsy), mRNA.

Price & Availability↑

CloneID	OFa15111
Clone ID Related Accession (Same CDS sequence)	NM_078886.3
Accession Version	NM_078886.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	clumsy, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_078886.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAAAAA TGGTTTGCAA TTTGATTTCC CAAGGAGTAA TCGCCATTTT TGGACCCAGC 
ACAGGCAGCA GTTCAGACAT AATTGCCTCA ATATGCGACA CACTCGATAT TCCGCACATA 
GTATATGACT GGATACCCAA TGAATCTATC CCAGATCGGG AACACTCTAC AATGACTCTT 
AATGTTCATC CCGATAACCT CTTGTTGTCC CAGGGGCTGG CGGAGATTGT GCAGAGCTTT 
GCCTGGCGTA GCTTTACAGT TGTCTATGAG ACTGACAAGG AGCTTCAACA ACTTCAGGAT 
ATTTTGCAAG TCGGTGAGCC CATCAGCAAT CCAACCACAG TAAAACAACT GGGGCCGGGT 
GACGACCACC GGCCTTTTTT AAAGGAAATC AAACTCTCTA CCGATAACTG CCTCATCCTA 
CATTGTGCCC CCGACAATTT ATTAAAAATA TTGCAACAGG CAAATGAGCT AAAAATGTTG 
GGCGAGTATC AGAGCGTTTT CATACCACTC CTGGACACCC ATTCCATCGA TTTTGGTGAA 
CTTTCCGGGG TCGAGGCCAA CATAACTACG GTTCGACTAA TGGATCCATC CGATTTTCAC 
GTGAAAAATG TAGTGCACGA CTGGGAAGAA CGCGAAAAAC GGGAGGGACG CTACTTTAAA 
GTCGACCCAA ATAGAGTCAA ATCCCAAATG ATTCTGCTCA ATGACGCCGT TTGGCTCTTT 
TCTAAGGGAC TTACCGAACT CGGAATTTTC GAGGAGCTCA CCGCTCCCGA TCTAGAATGT 
AGGCGAAAAA AACCGTGGCC ATTTGGTAAA CGCATTATTG AGTTCATAAA GGCGCGGTCC 
GAGGAGACTT CGACGGGACG AATTGACTTT AATGAGAATG GACAAAGAAG TTTTTTCACC 
CTGCGTTTTA TGGAATTAAA CTCTGATGGC TTTTTGGATT TAGCCACTTG GGATCCAGTG 
AACGGATTGG ATGTGCTTAA TGACGACGAA GAGAGCGAGA AGAGAGTTGG CCAGAAGCTG 
TCGAACAAAA CATTTATTGT ATCCTCACGC CTGGGAGCCC CATTCCTTAC ACTCCGAGAG 
CCCCAGGAAG GTGAAATCCT GACGGGGAAT TCCCGTTACG AGGGATACTC TATAGACTTA 
ATCAACGAAA TTGCCAAAAT GCTGAACTTT AAGTTCGAAT TCCGAATGTC CCCCGACGGA 
AAGTACGGTG CTCTAAATAA GGTGACCCAG ACCTGGGATG GCATAGTGAG GCAACTGATT 
GATGGGAATG CTGACCTTGG AATCTGCGAT TTGACAATGA CGTCATCCCG ACGTCAGGCA 
GTTGACTTTA CACCTCCATT CATGACCCTA GGAATCAGCA TACTGTTTTC GAAGCCTCCC 
ACACCACCCA CTGACTTGTT TTCGTTTTTA TCTCCATTCT CCTTGGATGT GTGGATCTAC 
ATGGGCTCCG CATATCTCTT TATTTCCCTT CTTTTGTTCG CATTGGCTCG GATGGCGCCC 
GACGACTGGG AAAACCCTCA TCCCTGCAAG GAACCCGAAG AAGTGGAGAA CATATGGTCG 
ATTATGAACA CTACATGGCT CTCGATAGGC TCATTGATGG GGCAAGGCTG TGACATTCTG 
CCGAAAGCTG CCTCAACTCG GTTGGTCACT GGAATGTGGT GGTTCTTCGC TCTAATGATG 
CTGAATTCCT ACACGGCTAA TCTGGCTGCC TTTCTAACCA ATTCTCGGCA GGCGAACTCC 
ATCAACAGTG CCGAGGATCT GGCTGCACAA AGTAAAATTA AATACGGTGC TATGGCTGGT 
GGTTCCACAA TGGGGTTCTT TCGGGACTCG AACTTTAGCA CTTACCAGAA GATGTGGACC 
GCCATGGAAA GCGCCAGTCC TTCAGTTTTC ACAAAAACCA ACGACGAGGG CGTCGAGAGG 
GTACAAAAAG GCAAGAATTT ATACGCCTTT TTAATGGAGT CCACCACATT GGAGTACAAT 
GTGGAACGAA AGTGCGACCT GGTGCAGATC GGTGGCTGGC TAGACTACAA GAGCTATGGC 
ATAGCAATGC CCTTCACTCC ACTGCTTGCC AGGTTACACA AGTCCAATGG CCTGATGGCA 
CTTTGCCCTT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523610.2
CDS	370..2484
Translation	MEKMVCNLISQGVIAIFGPSTGSSSDIIASICDTLDIPHIVYDWIPNESIPDREHSTMTLNVHPDNLLLSQGLAEIVQSFAWRSFTVVYETDKELQQLQDILQVGEPISNPTTVKQLGPGDDHRPFLKEIKLSTDNCLILHCAPDNLLKILQQANELKMLGEYQSVFIPLLDTHSIDFGELSGVEANITTVRLMDPSDFHVKNVVHDWEEREKREGRYFKVDPNRVKSQMILLNDAVWLFSKGLTELGIFEELTAPDLECRRKKPWPFGKRIIEFIKARSEETSTGRIDFNENGQRSFFTLRFMELNSDGFLDLATWDPVNGLDVLNDDEESEKRVGQKLSNKTFIVSSRLGAPFLTLREPQEGEILTGNSRYEGYSIDLINEIAKMLNFKFEFRMSPDGKYGALNKVTQTWDGIVRQLIDGNADLGICDLTMTSSRRQAVDFTPPFMTLGISILFSKPPTPPTDLFSFLSPFSLDVWIYMGSAYLFISLLLFALARMAPDDWENPHPCKEPEEVENIWSIMNTTWLSIGSLMGQGCDILPKAASTRLVTGMWWFFALMMLNSYTANLAAFLTNSRQANSINSAEDLAAQSKIKYGAMAGGSTMGFFRDSNFSTYQKMWTAMESASPSVFTKTNDEGVERVQKGKNLYAFLMESTTLEYNVERKCDLVQIGGWLDYKSYGIAMPFTPLLARLHKSNGLMALCPF

Target ORF information:

RefSeq Version	NM_078886.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster clumsy, transcript variant A (clumsy), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078886.3

ORF Insert Sequence:

ATGGAAAAAA TGGTTTGCAA TTTGATTTCC CAAGGAGTAA TCGCCATTTT TGGACCCAGC 
ACAGGCAGCA GTTCAGACAT AATTGCCTCA ATATGCGACA CACTCGATAT TCCGCACATA 
GTATATGACT GGATACCCAA TGAATCTATC CCAGATCGGG AACACTCTAC AATGACTCTT 
AATGTTCATC CCGATAACCT CTTGTTGTCC CAGGGGCTGG CGGAGATTGT GCAGAGCTTT 
GCCTGGCGTA GCTTTACAGT TGTCTATGAG ACTGACAAGG AGCTTCAACA ACTTCAGGAT 
ATTTTGCAAG TCGGTGAGCC CATCAGCAAT CCAACCACAG TAAAACAACT GGGGCCGGGT 
GACGACCACC GGCCTTTTTT AAAGGAAATC AAACTCTCTA CCGATAACTG CCTCATCCTA 
CATTGTGCCC CCGACAATTT ATTAAAAATA TTGCAACAGG CAAATGAGCT AAAAATGTTG 
GGCGAGTATC AGAGCGTTTT CATACCACTC CTGGACACCC ATTCCATCGA TTTTGGTGAA 
CTTTCCGGGG TCGAGGCCAA CATAACTACG GTTCGACTAA TGGATCCATC CGATTTTCAC 
GTGAAAAATG TAGTGCACGA CTGGGAAGAA CGCGAAAAAC GGGAGGGACG CTACTTTAAA 
GTCGACCCAA ATAGAGTCAA ATCCCAAATG ATTCTGCTCA ATGACGCCGT TTGGCTCTTT 
TCTAAGGGAC TTACCGAACT CGGAATTTTC GAGGAGCTCA CCGCTCCCGA TCTAGAATGT 
AGGCGAAAAA AACCGTGGCC ATTTGGTAAA CGCATTATTG AGTTCATAAA GGCGCGGTCC 
GAGGAGACTT CGACGGGACG AATTGACTTT AATGAGAATG GACAAAGAAG TTTTTTCACC 
CTGCGTTTTA TGGAATTAAA CTCTGATGGC TTTTTGGATT TAGCCACTTG GGATCCAGTG 
AACGGATTGG ATGTGCTTAA TGACGACGAA GAGAGCGAGA AGAGAGTTGG CCAGAAGCTG 
TCGAACAAAA CATTTATTGT ATCCTCACGC CTGGGAGCCC CATTCCTTAC ACTCCGAGAG 
CCCCAGGAAG GTGAAATCCT GACGGGGAAT TCCCGTTACG AGGGATACTC TATAGACTTA 
ATCAACGAAA TTGCCAAAAT GCTGAACTTT AAGTTCGAAT TCCGAATGTC CCCCGACGGA 
AAGTACGGTG CTCTAAATAA GGTGACCCAG ACCTGGGATG GCATAGTGAG GCAACTGATT 
GATGGGAATG CTGACCTTGG AATCTGCGAT TTGACAATGA CGTCATCCCG ACGTCAGGCA 
GTTGACTTTA CACCTCCATT CATGACCCTA GGAATCAGCA TACTGTTTTC GAAGCCTCCC 
ACACCACCCA CTGACTTGTT TTCGTTTTTA TCTCCATTCT CCTTGGATGT GTGGATCTAC 
ATGGGCTCCG CATATCTCTT TATTTCCCTT CTTTTGTTCG CATTGGCTCG GATGGCGCCC 
GACGACTGGG AAAACCCTCA TCCCTGCAAG GAACCCGAAG AAGTGGAGAA CATATGGTCG 
ATTATGAACA CTACATGGCT CTCGATAGGC TCATTGATGG GGCAAGGCTG TGACATTCTG 
CCGAAAGCTG CCTCAACTCG GTTGGTCACT GGAATGTGGT GGTTCTTCGC TCTAATGATG 
CTGAATTCCT ACACGGCTAA TCTGGCTGCC TTTCTAACCA ATTCTCGGCA GGCGAACTCC 
ATCAACAGTG CCGAGGATCT GGCTGCACAA AGTAAAATTA AATACGGTGC TATGGCTGGT 
GGTTCCACAA TGGGGTTCTT TCGGGACTCG AACTTTAGCA CTTACCAGAA GATGTGGACC 
GCCATGGAAA GCGCCAGTCC TTCAGTTTTC ACAAAAACCA ACGACGAGGG CGTCGAGAGG 
GTACAAAAAG GCAAGAATTT ATACGCCTTT TTAATGGAGT CCACCACATT GGAGTACAAT 
GTGGAACGAA AGTGCGACCT GGTGCAGATC GGTGGCTGGC TAGACTACAA GAGCTATGGC 
ATAGCAATGC CCTTCACTCC ACTGCTTGCC AGGTTACACA AGTCCAATGG CCTGATGGCA 
CTTTGCCCTT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

clumsy ( NM_001042908.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001036373.1 Drosophila melanogaster clumsy, transcript variant B (clumsy), mRNA.

Price & Availability↑

CloneID	OFa15110
Clone ID Related Accession (Same CDS sequence)	NM_001042908.2
Accession Version	NM_001042908.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3009bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	clumsy, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001042908.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTATTCTC TATTCCTCAC TCACTTTCTT CTGATCGCGT TGCCAGTCCT CGCAGACATA 
GACAGAAGTC AGTTTATGGT GGGTAGCATC TTCACATCAG ACAAAGATGA GTCGGAAATC 
GCATTCCGAA CAGCGGTAGA CCGTGCAAAC ATATTGGAGC GGAACGTAGA GTTGGTTCCA 
ATTGTGGTGT ATGCTAACAC CGATGATAGT TTCATCATGG AAAAAATGGT TTGCAATTTG 
ATTTCCCAAG GAGTAATCGC CATTTTTGGA CCCAGCACAG GCAGCAGTTC AGACATAATT 
GCCTCAATAT GCGACACACT CGATATTCCG CACATAGTAT ATGACTGGAT ACCCAATGAA 
TCTATCCCAG ATCGGGAACA CTCTACAATG ACTCTTAATG TTCATCCCGA TAACCTCTTG 
TTGTCCCAGG GGCTGGCGGA GATTGTGCAG AGCTTTGCCT GGCGTAGCTT TACAGTTGTC 
TATGAGACTG ACAAGGAGCT TCAACAACTT CAGGATATTT TGCAAGTCGG TGAGCCCATC 
AGCAATCCAA CCACAGTAAA ACAACTGGGG CCGGGTGACG ACCACCGGCC TTTTTTAAAG 
GAAATCAAAC TCTCTACCGA TAACTGCCTC ATCCTACATT GTGCCCCCGA CAATTTATTA 
AAAATATTGC AACAGGCAAA TGAGCTAAAA ATGTTGGGCG AGTATCAGAG CGTTTTCATA 
CCACTCCTGG ACACCCATTC CATCGATTTT GGTGAACTTT CCGGGGTCGA GGCCAACATA 
ACTACGGTTC GACTAATGGA TCCATCCGAT TTTCACGTGA AAAATGTAGT GCACGACTGG 
GAAGAACGCG AAAAACGGGA GGGACGCTAC TTTAAAGTCG ACCCAAATAG AGTCAAATCC 
CAAATGATTC TGCTCAATGA CGCCGTTTGG CTCTTTTCTA AGGGACTTAC CGAACTCGGA 
ATTTTCGAGG AGCTCACCGC TCCCGATCTA GAATGTAGGC GAAAAAAACC GTGGCCATTT 
GGTAAACGCA TTATTGAGTT CATAAAGGCG CGGTCCGAGG AGACTTCGAC GGGACGAATT 
GACTTTAATG AGAATGGACA AAGAAGTTTT TTCACCCTGC GTTTTATGGA ATTAAACTCT 
GATGGCTTTT TGGATTTAGC CACTTGGGAT CCAGTGAACG GATTGGATGT GCTTAATGAC 
GACGAAGAGA GCGAGAAGAG AGTTGGCCAG AAGCTGTCGA ACAAAACATT TATTGTATCC 
TCACGCCTGG GAGCCCCATT CCTTACACTC CGAGAGCCCC AGGAAGGTGA AATCCTGACG 
GGGAATTCCC GTTACGAGGG ATACTCTATA GACTTAATCA ACGAAATTGC CAAAATGCTG 
AACTTTAAGT TCGAATTCCG AATGTCCCCC GACGGAAAGT ACGGTGCTCT AAATAAGGTG 
ACCCAGACCT GGGATGGCAT AGTGAGGCAA CTGATTGATG GGAATGCTGA CCTTGGAATC 
TGCGATTTGA CAATGACGTC ATCCCGACGT CAGGCAGTTG ACTTTACACC TCCATTCATG 
ACCCTAGGAA TCAGCATACT GTTTTCGAAG CCTCCCACAC CACCCACTGA CTTGTTTTCG 
TTTTTATCTC CATTCTCCTT GGATGTGTGG ATCTACATGG GCTCCGCATA TCTCTTTATT 
TCCCTTCTTT TGTTCGCATT GGCTCGGATG GCGCCCGACG ACTGGGAAAA CCCTCATCCC 
TGCAAGGAAC CCGAAGAAGT GGAGAACATA TGGTCGATTA TGAACACTAC ATGGCTCTCG 
ATAGGCTCAT TGATGGGGCA AGGCTGTGAC ATTCTGCCGA AAGCTGCCTC AACTCGGTTG 
GTCACTGGAA TGTGGTGGTT CTTCGCTCTA ATGATGCTGA ATTCCTACAC GGCTAATCTG 
GCTGCCTTTC TAACCAATTC TCGGCAGGCG AACTCCATCA ACAGTGCCGA GGATCTGGCT 
GCACAAAGTA AAATTAAATA CGGTGCTATG GCTGGTGGTT CCACAATGGG GTTCTTTCGG 
GACTCGAACT TTAGCACTTA CCAGAAGATG TGGACCGCCA TGGAAAGCGC CAGTCCTTCA 
GTTTTCACAA AAACCAACGA CGAGGGCGTC GAGAGGGTAC AAAAAGGCAA GAATTTATAC 
GCCTTTTTAA TGGAGTCCAC CACATTGGAG TACAATGTGG AACGAAAGTG CGACCTGGTG 
CAGATCGGTG GCTGGCTAGA CTACAAGAGC TATGGCATAG CAATGCCCTT CAATTCTCCG 
TATCGCAAAC AGATCAGTGC GGCAGTGCTG AAGTTGGGCG AACTTGGACA GCTGGCGGAA 
CTAAAGCGAA AGTGGTGGAA GGAGATGCAC GGCGGCGGGA ACTGCGAAAA GAGCGACGAA 
GATGGGGGAG ACACGCCAGA GCTTGGATTG GAAAACGTGG GTGGAGTCTT CCTGGTGCTT 
GGTCTCGGCT TGCTTTCAGC AATGGTTTTG GGATGCACGG AATTTCTATG GAACGTGAAA 
TCGGTAGCAA TTGAAGAAAA GATCTCGCTT AAGGAGGCTT TTAAATCAGA GGCTTTGTTT 
GCGGCTAGAA TATGGATAAC CACTAAACCT GTACATACAA GTTCTGAGTC TGGAAGCTCA 
AATTCGTCTA GTTCCTCGTC AAGCCGCTCC AAGCATTCTT TCAAGTCGCA GGGATTGTCA 
ATGAAAAGCC TTAAAAGCTC AGGATATCAG GACGTAGAAG CTTCAGTCCA CAGCAAACTA 
AAAAAAATCG GTTCCATGTT CTCACTAAAG TCACAGAAGA CAGTCACTCC ACCTCCAGAG 
ATAGGCTGGA AACTGGACAA GTCAACACAA ATCGATGTAG TTCCAACATC TGATGTAGAT 
CAAGAATTGA TACCGGAGGT AGAACCACAT CTACCACATC GGCACCATCA TCACCATCAT 
CATCGCCATC ATCATCACCA TCATCAACCC GATCAGGAAC ATGATAGGAA TCCCTCGCCT 
CCTGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036373.1
CDS	158..3166
Translation	MYSLFLTHFLLIALPVLADIDRSQFMVGSIFTSDKDESEIAFRTAVDRANILERNVELVPIVVYANTDDSFIMEKMVCNLISQGVIAIFGPSTGSSSDIIASICDTLDIPHIVYDWIPNESIPDREHSTMTLNVHPDNLLLSQGLAEIVQSFAWRSFTVVYETDKELQQLQDILQVGEPISNPTTVKQLGPGDDHRPFLKEIKLSTDNCLILHCAPDNLLKILQQANELKMLGEYQSVFIPLLDTHSIDFGELSGVEANITTVRLMDPSDFHVKNVVHDWEEREKREGRYFKVDPNRVKSQMILLNDAVWLFSKGLTELGIFEELTAPDLECRRKKPWPFGKRIIEFIKARSEETSTGRIDFNENGQRSFFTLRFMELNSDGFLDLATWDPVNGLDVLNDDEESEKRVGQKLSNKTFIVSSRLGAPFLTLREPQEGEILTGNSRYEGYSIDLINEIAKMLNFKFEFRMSPDGKYGALNKVTQTWDGIVRQLIDGNADLGICDLTMTSSRRQAVDFTPPFMTLGISILFSKPPTPPTDLFSFLSPFSLDVWIYMGSAYLFISLLLFALARMAPDDWENPHPCKEPEEVENIWSIMNTTWLSIGSLMGQGCDILPKAASTRLVTGMWWFFALMMLNSYTANLAAFLTNSRQANSINSAEDLAAQSKIKYGAMAGGSTMGFFRDSNFSTYQKMWTAMESASPSVFTKTNDEGVERVQKGKNLYAFLMESTTLEYNVERKCDLVQIGGWLDYKSYGIAMPFNSPYRKQISAAVLKLGELGQLAELKRKWWKEMHGGGNCEKSDEDGGDTPELGLENVGGVFLVLGLGLLSAMVLGCTEFLWNVKSVAIEEKISLKEAFKSEALFAARIWITTKPVHTSSESGSSNSSSSSSSRSKHSFKSQGLSMKSLKSSGYQDVEASVHSKLKKIGSMFSLKSQKTVTPPPEIGWKLDKSTQIDVVPTSDVDQELIPEVEPHLPHRHHHHHHHRHHHHHHQPDQEHDRNPSPPE

Target ORF information:

RefSeq Version	NM_001042908.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster clumsy, transcript variant B (clumsy), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042908.2

ORF Insert Sequence:

ATGTATTCTC TATTCCTCAC TCACTTTCTT CTGATCGCGT TGCCAGTCCT CGCAGACATA 
GACAGAAGTC AGTTTATGGT GGGTAGCATC TTCACATCAG ACAAAGATGA GTCGGAAATC 
GCATTCCGAA CAGCGGTAGA CCGTGCAAAC ATATTGGAGC GGAACGTAGA GTTGGTTCCA 
ATTGTGGTGT ATGCTAACAC CGATGATAGT TTCATCATGG AAAAAATGGT TTGCAATTTG 
ATTTCCCAAG GAGTAATCGC CATTTTTGGA CCCAGCACAG GCAGCAGTTC AGACATAATT 
GCCTCAATAT GCGACACACT CGATATTCCG CACATAGTAT ATGACTGGAT ACCCAATGAA 
TCTATCCCAG ATCGGGAACA CTCTACAATG ACTCTTAATG TTCATCCCGA TAACCTCTTG 
TTGTCCCAGG GGCTGGCGGA GATTGTGCAG AGCTTTGCCT GGCGTAGCTT TACAGTTGTC 
TATGAGACTG ACAAGGAGCT TCAACAACTT CAGGATATTT TGCAAGTCGG TGAGCCCATC 
AGCAATCCAA CCACAGTAAA ACAACTGGGG CCGGGTGACG ACCACCGGCC TTTTTTAAAG 
GAAATCAAAC TCTCTACCGA TAACTGCCTC ATCCTACATT GTGCCCCCGA CAATTTATTA 
AAAATATTGC AACAGGCAAA TGAGCTAAAA ATGTTGGGCG AGTATCAGAG CGTTTTCATA 
CCACTCCTGG ACACCCATTC CATCGATTTT GGTGAACTTT CCGGGGTCGA GGCCAACATA 
ACTACGGTTC GACTAATGGA TCCATCCGAT TTTCACGTGA AAAATGTAGT GCACGACTGG 
GAAGAACGCG AAAAACGGGA GGGACGCTAC TTTAAAGTCG ACCCAAATAG AGTCAAATCC 
CAAATGATTC TGCTCAATGA CGCCGTTTGG CTCTTTTCTA AGGGACTTAC CGAACTCGGA 
ATTTTCGAGG AGCTCACCGC TCCCGATCTA GAATGTAGGC GAAAAAAACC GTGGCCATTT 
GGTAAACGCA TTATTGAGTT CATAAAGGCG CGGTCCGAGG AGACTTCGAC GGGACGAATT 
GACTTTAATG AGAATGGACA AAGAAGTTTT TTCACCCTGC GTTTTATGGA ATTAAACTCT 
GATGGCTTTT TGGATTTAGC CACTTGGGAT CCAGTGAACG GATTGGATGT GCTTAATGAC 
GACGAAGAGA GCGAGAAGAG AGTTGGCCAG AAGCTGTCGA ACAAAACATT TATTGTATCC 
TCACGCCTGG GAGCCCCATT CCTTACACTC CGAGAGCCCC AGGAAGGTGA AATCCTGACG 
GGGAATTCCC GTTACGAGGG ATACTCTATA GACTTAATCA ACGAAATTGC CAAAATGCTG 
AACTTTAAGT TCGAATTCCG AATGTCCCCC GACGGAAAGT ACGGTGCTCT AAATAAGGTG 
ACCCAGACCT GGGATGGCAT AGTGAGGCAA CTGATTGATG GGAATGCTGA CCTTGGAATC 
TGCGATTTGA CAATGACGTC ATCCCGACGT CAGGCAGTTG ACTTTACACC TCCATTCATG 
ACCCTAGGAA TCAGCATACT GTTTTCGAAG CCTCCCACAC CACCCACTGA CTTGTTTTCG 
TTTTTATCTC CATTCTCCTT GGATGTGTGG ATCTACATGG GCTCCGCATA TCTCTTTATT 
TCCCTTCTTT TGTTCGCATT GGCTCGGATG GCGCCCGACG ACTGGGAAAA CCCTCATCCC 
TGCAAGGAAC CCGAAGAAGT GGAGAACATA TGGTCGATTA TGAACACTAC ATGGCTCTCG 
ATAGGCTCAT TGATGGGGCA AGGCTGTGAC ATTCTGCCGA AAGCTGCCTC AACTCGGTTG 
GTCACTGGAA TGTGGTGGTT CTTCGCTCTA ATGATGCTGA ATTCCTACAC GGCTAATCTG 
GCTGCCTTTC TAACCAATTC TCGGCAGGCG AACTCCATCA ACAGTGCCGA GGATCTGGCT 
GCACAAAGTA AAATTAAATA CGGTGCTATG GCTGGTGGTT CCACAATGGG GTTCTTTCGG 
GACTCGAACT TTAGCACTTA CCAGAAGATG TGGACCGCCA TGGAAAGCGC CAGTCCTTCA 
GTTTTCACAA AAACCAACGA CGAGGGCGTC GAGAGGGTAC AAAAAGGCAA GAATTTATAC 
GCCTTTTTAA TGGAGTCCAC CACATTGGAG TACAATGTGG AACGAAAGTG CGACCTGGTG 
CAGATCGGTG GCTGGCTAGA CTACAAGAGC TATGGCATAG CAATGCCCTT CAATTCTCCG 
TATCGCAAAC AGATCAGTGC GGCAGTGCTG AAGTTGGGCG AACTTGGACA GCTGGCGGAA 
CTAAAGCGAA AGTGGTGGAA GGAGATGCAC GGCGGCGGGA ACTGCGAAAA GAGCGACGAA 
GATGGGGGAG ACACGCCAGA GCTTGGATTG GAAAACGTGG GTGGAGTCTT CCTGGTGCTT 
GGTCTCGGCT TGCTTTCAGC AATGGTTTTG GGATGCACGG AATTTCTATG GAACGTGAAA 
TCGGTAGCAA TTGAAGAAAA GATCTCGCTT AAGGAGGCTT TTAAATCAGA GGCTTTGTTT 
GCGGCTAGAA TATGGATAAC CACTAAACCT GTACATACAA GTTCTGAGTC TGGAAGCTCA 
AATTCGTCTA GTTCCTCGTC AAGCCGCTCC AAGCATTCTT TCAAGTCGCA GGGATTGTCA 
ATGAAAAGCC TTAAAAGCTC AGGATATCAG GACGTAGAAG CTTCAGTCCA CAGCAAACTA 
AAAAAAATCG GTTCCATGTT CTCACTAAAG TCACAGAAGA CAGTCACTCC ACCTCCAGAG 
ATAGGCTGGA AACTGGACAA GTCAACACAA ATCGATGTAG TTCCAACATC TGATGTAGAT 
CAAGAATTGA TACCGGAGGT AGAACCACAT CTACCACATC GGCACCATCA TCACCATCAT 
CATCGCCATC ATCATCACCA TCATCAACCC GATCAGGAAC ATGATAGGAA TCCCTCGCCT 
CCTGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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clumsy cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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