Nbr cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nbr
Entrez Gene ID	35385
Full Name	Nibbler
Synonyms	CG9247,Dmel\CG9247,nbr
General protein information	Preferred Names Nibbler Names CG9247 gene product from transcript CG9247-RA CG9247-PA Nbr-PA nibbler
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 39A6-39A7

mRNA and Protein(s)

mRNA	Protein	Name
NM_136250.4	NP_610094.1	nibbler

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	EXD3	XP_005625106.1
Danio rerio (zebrafish)	exd3	XP_003199355.2
Drosophila melanogaster (fruit fly)	Nbr	NP_610094.1
Xenopus tropicalis (tropical clawed frog)	exd3	XP_002942768.2
Homo sapiens (human)	EXD3	NP_060290.3
Gallus gallus (chicken)	EXD3	XP_415556.4
Caenorhabditis elegans (roundworm)	mut-7	NP_499105.1
Caenorhabditis elegans (roundworm)	ZK1098.3	NP_499104.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

antagonistic roles of Nbr and Hen1 define a mechanism to modulate piRNA 3' ends.
Title: Antagonistic roles of Nibbler and Hen1 in modulating piRNA 3' ends in Drosophila.

indicate a role of Nibbler on age-associated processes and to modulate length of multiple classes of small RNAs including miRNAs and piRNAs in Drosophila
Title: The exonuclease Nibbler regulates age-associated traits and modulates piRNA length in Drosophila.

The study provides a molecular explanation for the previously reported heterogeneity of miRNA 3' ends and proposes a model in which Nibbler (CG9247)converts miRNAs into isoforms that are compatible with the preferred length of Ago1-bound small RNAs.
Title: The 3'-to-5' exoribonuclease Nibbler shapes the 3' ends of microRNAs bound to Drosophila Argonaute1.

An RNA interference screen identified a putative 3'-5' exoribonuclease CG9247/nibbler essential for the generation of smaller isoforms of the microRNA miR-34. Nibbler interacts with Argonaute1 and processes miR-34 within the RNA-induced silencing complex.
Title: The exoribonuclease Nibbler controls 3' end processing of microRNAs in Drosophila.

The following Nbr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nbr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa15105	NM_136250.4 Latest version!	Drosophila melanogaster nibbler (Nbr), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nbr ( NM_136250.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610094.1 Drosophila melanogaster nibbler (Nbr), mRNA.

Price & Availability↑

CloneID	OFa15105
Clone ID Related Accession (Same CDS sequence)	NM_136250.4
Accession Version	NM_136250.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nibbler
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_136250.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCACGCA AGAGCCACAT GTACAACGCA ATACCCGCCG GCTTTGAGTC GGACGAGGAG 
AACATGGAGA ACCTAATGAG CAACCTGAAG ATCAAGCGCT TGGAGGACAT CACGACAGGT 
GCTGGAATCG ACGGCTGTAA CTTTGACGCC ACCTTGGACG CAAAGGCGGA GGAGTTTTTT 
AAGCTGTTCC GCGAGAAGTG GAACATGTAT AGCAAGAAAA AGTCGCCACA TCTGCGCCAG 
GAATTTGGAA GGGCTTTAAT GGGCCATCAG GATCCTTTGC TCCTGGCCCT GAAGATCTTC 
GCCAACTGCC CAGACAGCAG CAACATCAAG ACGAAGAGCT TGTCACATTT TGTGCTGGAC 
ACGGTTTGCA AGCTCCACAA GGATTTCCCG CATCTTGGCG AGGGCTGTGA TCCCAACACC 
AGCATGATAG CCTTTAACTT TGTCAAGACC TCCGGCTTGC TGGCCCTCAA CAATGCTGTT 
ATCCATGCCT ACAGTCTACG TCAGATCCGC GACCTACTGC TTCCCAAGTT GCGCGAACTC 
CTGGACAACG GGCTTTACAA AGAGGTCACC CAATGGTCCA TCAGTCTGCA GTTGACGCAC 
GAGTTCGACA TGCTGGAACT GGCTTTCCCG CTGATTGCAA TTGAGAAACT ACCACTGGCC 
GAGGAATATC TGGACCATGC CACACAGCAG AGGTTGCCCT TTGTCAAGTT CTTGGATTCG 
TTGCTGCACA AGGAGAAGTC GGTTTTAGAG CTCTGCGAGC ATCTACTCGA TCGGTACAAA 
AATCTCAAAA TTTCCCATAA CGTGCTCAGC TATCGGCCTA TGGCCAAGAT TGTGGCGCGA 
CTGGCCAAGA AGTATGGTTT TGACGACGCC GTCACGCCCA ACTATAAGTT CACGAAGACG 
TGCAGCTACT TGCATTACCT TTACCGCGAG TACGAGAAGA CGCGCATGAA CCTGGCCAGT 
TTCCGGGAGG TTGTGAGCGT CCATGCCTTT AACCACGAGC TGCGCACGGA CTTCGTAAAG 
TATTTGGCCT CTGCTGGAGC TCATTCTGAA GCCATCTACT GGTACACGGA GTTCAATATT 
GACCCGAAAG ATTGTCCGTT GGAGATCGAG ACGCAGGTAT CGCAAAACGG TGCTGGAAAA 
GCGTCAGGCT GGGAATCCCC CGGCAAGGAA CGCTGTCCTT CGAGTAGATG TGATATGTAC 
CTGACCATGG ACCTGCCCGA CGAGTGCCTT ATCATTGTGA ACAAGGCTGA TGAGTTCGAT 
CGTATGTTAT ACCATCTGCA GCAGGAGTGC GTCATATACC TGGATTCTGA ATGGATGCAG 
AGCGTGTGCG GAGACAATCA GCTGTGCGTG CTACAGATCG CCACCGGCCA CAATGTCTAC 
CTGATCGATT GCCTGGCGCG GGAGAGCTTA CGCTCGGAAC ACTGGCGCTT GCTGGGCGCG 
AATATCTTTA ATAACGTGAA CATCCGTAAG GTGGGGTTCT CTATGGTCAG TGATCTCAGT 
GTATTGCAGC GGTCACTGCC CCTGCAACTG CGCCTTCAAA TGCCTCACCA CTACTTGGAC 
CTTCGCAATC TCTGGTTGGA GTTAAAAAAG CAGCGCTTCG GCGTTGAACT TCCCTTCGGC 
AATGTTAACC GGGCAGGAGA CGCCCTTACG GATCTCTCAT TGGCATGCCT TGGCAAGAAA 
CTGAACAAAT CAAACCAGTG CTCAAACTGG GCCAATAGAC CGCTGCGTCG CGAGCAGATT 
CTTTATGCAG CCATCGATGC GCGGTGTTTG ATGCTGATCT ACAACACGCT CATTGAACGC 
GTGTCCTTCA TACAAGCGGT TATCGAAAAG AGCATCGCCA GCAATAACTT TCTCAGGCGG 
GGTGCCCATG TTAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610094.1
CDS	82..1959
Translation	MARKSHMYNAIPAGFESDEENMENLMSNLKIKRLEDITTGAGIDGCNFDATLDAKAEEFFKLFREKWNMYSKKKSPHLRQEFGRALMGHQDPLLLALKIFANCPDSSNIKTKSLSHFVLDTVCKLHKDFPHLGEGCDPNTSMIAFNFVKTSGLLALNNAVIHAYSLRQIRDLLLPKLRELLDNGLYKEVTQWSISLQLTHEFDMLELAFPLIAIEKLPLAEEYLDHATQQRLPFVKFLDSLLHKEKSVLELCEHLLDRYKNLKISHNVLSYRPMAKIVARLAKKYGFDDAVTPNYKFTKTCSYLHYLYREYEKTRMNLASFREVVSVHAFNHELRTDFVKYLASAGAHSEAIYWYTEFNIDPKDCPLEIETQVSQNGAGKASGWESPGKERCPSSRCDMYLTMDLPDECLIIVNKADEFDRMLYHLQQECVIYLDSEWMQSVCGDNQLCVLQIATGHNVYLIDCLARESLRSEHWRLLGANIFNNVNIRKVGFSMVSDLSVLQRSLPLQLRLQMPHHYLDLRNLWLELKKQRFGVELPFGNVNRAGDALTDLSLACLGKKLNKSNQCSNWANRPLRREQILYAAIDARCLMLIYNTLIERVSFIQAVIEKSIASNNFLRRGAHVK

Target ORF information:

RefSeq Version	NM_136250.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nibbler (Nbr), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136250.4

ORF Insert Sequence:

ATGGCACGCA AGAGCCACAT GTACAACGCA ATACCCGCCG GCTTTGAGTC GGACGAGGAG 
AACATGGAGA ACCTAATGAG CAACCTGAAG ATCAAGCGCT TGGAGGACAT CACGACAGGT 
GCTGGAATCG ACGGCTGTAA CTTTGACGCC ACCTTGGACG CAAAGGCGGA GGAGTTTTTT 
AAGCTGTTCC GCGAGAAGTG GAACATGTAT AGCAAGAAAA AGTCGCCACA TCTGCGCCAG 
GAATTTGGAA GGGCTTTAAT GGGCCATCAG GATCCTTTGC TCCTGGCCCT GAAGATCTTC 
GCCAACTGCC CAGACAGCAG CAACATCAAG ACGAAGAGCT TGTCACATTT TGTGCTGGAC 
ACGGTTTGCA AGCTCCACAA GGATTTCCCG CATCTTGGCG AGGGCTGTGA TCCCAACACC 
AGCATGATAG CCTTTAACTT TGTCAAGACC TCCGGCTTGC TGGCCCTCAA CAATGCTGTT 
ATCCATGCCT ACAGTCTACG TCAGATCCGC GACCTACTGC TTCCCAAGTT GCGCGAACTC 
CTGGACAACG GGCTTTACAA AGAGGTCACC CAATGGTCCA TCAGTCTGCA GTTGACGCAC 
GAGTTCGACA TGCTGGAACT GGCTTTCCCG CTGATTGCAA TTGAGAAACT ACCACTGGCC 
GAGGAATATC TGGACCATGC CACACAGCAG AGGTTGCCCT TTGTCAAGTT CTTGGATTCG 
TTGCTGCACA AGGAGAAGTC GGTTTTAGAG CTCTGCGAGC ATCTACTCGA TCGGTACAAA 
AATCTCAAAA TTTCCCATAA CGTGCTCAGC TATCGGCCTA TGGCCAAGAT TGTGGCGCGA 
CTGGCCAAGA AGTATGGTTT TGACGACGCC GTCACGCCCA ACTATAAGTT CACGAAGACG 
TGCAGCTACT TGCATTACCT TTACCGCGAG TACGAGAAGA CGCGCATGAA CCTGGCCAGT 
TTCCGGGAGG TTGTGAGCGT CCATGCCTTT AACCACGAGC TGCGCACGGA CTTCGTAAAG 
TATTTGGCCT CTGCTGGAGC TCATTCTGAA GCCATCTACT GGTACACGGA GTTCAATATT 
GACCCGAAAG ATTGTCCGTT GGAGATCGAG ACGCAGGTAT CGCAAAACGG TGCTGGAAAA 
GCGTCAGGCT GGGAATCCCC CGGCAAGGAA CGCTGTCCTT CGAGTAGATG TGATATGTAC 
CTGACCATGG ACCTGCCCGA CGAGTGCCTT ATCATTGTGA ACAAGGCTGA TGAGTTCGAT 
CGTATGTTAT ACCATCTGCA GCAGGAGTGC GTCATATACC TGGATTCTGA ATGGATGCAG 
AGCGTGTGCG GAGACAATCA GCTGTGCGTG CTACAGATCG CCACCGGCCA CAATGTCTAC 
CTGATCGATT GCCTGGCGCG GGAGAGCTTA CGCTCGGAAC ACTGGCGCTT GCTGGGCGCG 
AATATCTTTA ATAACGTGAA CATCCGTAAG GTGGGGTTCT CTATGGTCAG TGATCTCAGT 
GTATTGCAGC GGTCACTGCC CCTGCAACTG CGCCTTCAAA TGCCTCACCA CTACTTGGAC 
CTTCGCAATC TCTGGTTGGA GTTAAAAAAG CAGCGCTTCG GCGTTGAACT TCCCTTCGGC 
AATGTTAACC GGGCAGGAGA CGCCCTTACG GATCTCTCAT TGGCATGCCT TGGCAAGAAA 
CTGAACAAAT CAAACCAGTG CTCAAACTGG GCCAATAGAC CGCTGCGTCG CGAGCAGATT 
CTTTATGCAG CCATCGATGC GCGGTGTTTG ATGCTGATCT ACAACACGCT CATTGAACGC 
GTGTCCTTCA TACAAGCGGT TATCGAAAAG AGCATCGCCA GCAATAACTT TCTCAGGCGG 
GGTGCCCATG TTAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nbr cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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