spi cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	spi
Entrez Gene ID	35253
Full Name	spitz
Synonyms	CG10334,CT29014,Dmel\CG10334,EP(2)2378,Spi,Spitz,anon-WO0118547.158,l(2)01068,l(2)37Fa,l(2)s3547,s-spi,sSpi
General protein information	Preferred Names spitz Names CG10334 gene product from transcript CG10334-RF CG10334-PA CG10334-PB CG10334-PE CG10334-PF CG10334-PG epidermal growth factor protein spi-PA spi-PB spi-PE spi-PF spi-PG
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 37F2-37F2\|2-54 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_134292.3	NP_599119.2	spitz, isoform E
NM_134293.3	NP_599120.2	spitz, isoform B
NM_057561.6	NP_476909.2	spitz, isoform F
NM_001032110.2	NP_001027281.1	spitz, isoform G
NM_134291.5	NP_599118.2	spitz, isoform A

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	spi	NP_599120.2
Drosophila melanogaster (fruit fly)	Krn	NP_524129.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We provide evidence that miR-8-positive glia express Spitz, a transforming growth factor alpha-like ligand that triggers epidermal growth factor receptor activation to promote neuroepithelial proliferation and neuroblast formation.
Title: Conserved miR-8/miR-200 defines a glial niche that controls neuroepithelial expansion and neuroblast transition.

Study describes crystal structures of s-dEGFR bound to its growth factor ligand Spitz, showing how occupying one binding site in a receptor dimer impairs ligand binding to the second site in an asymmetric complex.
Title: Structural basis for negative cooperativity in growth factor binding to an EGF receptor.

Export of Spitz-GFP from the endoplasmic reticulum, as well as the trafficking of Spitz-GFP vesicles, depends on both Star and dynein.
Title: Dynein and Star interact in EGFR signaling and ligand trafficking.

1.6-A resolution crystal structure of Argos bound to Spitz
Title: Structural basis for EGFR ligand sequestration by Argos.

Germ cells signal somatic support cells via the Egf class ligand Spitz.
Title: Antagonistic roles of Rac and Rho in organizing the germ cell microenvironment.

The following spi gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spi cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa16903	NM_134292.3 Latest version!	Drosophila melanogaster spitz (spi), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa16903	NM_134291.5 Latest version!	Drosophila melanogaster spitz (spi), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa16903	NM_057561.6 Latest version!	Drosophila melanogaster spitz (spi), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa16903	NM_134293.3 Latest version!	Drosophila melanogaster spitz (spi), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa16903	NM_001032110.2 Latest version!	Drosophila melanogaster spitz (spi), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

spi ( NM_134292.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_599119.2 Drosophila melanogaster spitz (spi), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa16903
Clone ID Related Accession (Same CDS sequence)	NM_134292.3 , NM_134293.3 , NM_057561.6 , NM_001032110.2 , NM_134291.5
Accession Version	NM_134292.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spitz, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On May 8, 2012 this sequence version replaced NM_134292.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATTCCA CAATGAGTGT ACAACATGGG CTGGTTGCCC TGGTACTGAT CGGCTGTCTG 
GCACATCCGT GGCACGTCGA GGCCTGCTCC AGCCGTACGG TGCCCAAGCC GCGCTCTTCG 
ATCTCCTCGT CCATGTCCGG CACGGCATTG CCGCCGACCC AGGCTCCAGT CACTAGTAGC 
ACCACAATGC GCACCACCAC GACGACCACG CCCAGGCCCA ATATTACATT CCCCACATAC 
AAATGTCCGG AAACCTTCGA TGCCTGGTAC TGTTTGAACG ATGCCCATTG CTTTGCGGTG 
AAGATAGCCG ATCTACCGGT TTACAGCTGC GAGTGCGCGA TTGGCTTTAT GGGACAGCGA 
TGCGAATACA AGGAGATCGA CAATACTTAC CTGCCCAAGA GGCCGCGTCC GATGTTGGAG 
AAGGCGAGCA TTGCCAGTGG AGCCATGTGT GCCCTGGTAT TTATGCTGTT TGTCTGCCTG 
GCCTTCTATT TGCGCTTCGA GCAGCGGGCT GCCAAGAAGG CCTACGAACT GGAGCAGGAA 
CTGCAGCAGG AATACGACGA TGACGACGGC CAGTGCGAGT GCTGCCGCAA CCGGTGCTGT 
CCAGATGGCC AGGAGCCAGT CATTCTGGAG CGCAAGCTAC CCTACCACAT GCGGCTGGAG 
CACGCGCTAA TGTCCTTCGC CATTCGACGC AGCAACAAGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_599119.2
CDS	341..1045
Translation	MHSTMSVQHGLVALVLIGCLAHPWHVEACSSRTVPKPRSSISSSMSGTALPPTQAPVTSSTTMRTTTTTTPRPNITFPTYKCPETFDAWYCLNDAHCFAVKIADLPVYSCECAIGFMGQRCEYKEIDNTYLPKRPRPMLEKASIASGAMCALVFMLFVCLAFYLRFEQRAAKKAYELEQELQQEYDDDDGQCECCRNRCCPDGQEPVILERKLPYHMRLEHALMSFAIRRSNKL

Target ORF information:

RefSeq Version	NM_134292.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spitz (spi), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134292.3

ORF Insert Sequence:

ATGCATTCCA CAATGAGTGT ACAACATGGG CTGGTTGCCC TGGTACTGAT CGGCTGTCTG 
GCACATCCGT GGCACGTCGA GGCCTGCTCC AGCCGTACGG TGCCCAAGCC GCGCTCTTCG 
ATCTCCTCGT CCATGTCCGG CACGGCATTG CCGCCGACCC AGGCTCCAGT CACTAGTAGC 
ACCACAATGC GCACCACCAC GACGACCACG CCCAGGCCCA ATATTACATT CCCCACATAC 
AAATGTCCGG AAACCTTCGA TGCCTGGTAC TGTTTGAACG ATGCCCATTG CTTTGCGGTG 
AAGATAGCCG ATCTACCGGT TTACAGCTGC GAGTGCGCGA TTGGCTTTAT GGGACAGCGA 
TGCGAATACA AGGAGATCGA CAATACTTAC CTGCCCAAGA GGCCGCGTCC GATGTTGGAG 
AAGGCGAGCA TTGCCAGTGG AGCCATGTGT GCCCTGGTAT TTATGCTGTT TGTCTGCCTG 
GCCTTCTATT TGCGCTTCGA GCAGCGGGCT GCCAAGAAGG CCTACGAACT GGAGCAGGAA 
CTGCAGCAGG AATACGACGA TGACGACGGC CAGTGCGAGT GCTGCCGCAA CCGGTGCTGT 
CCAGATGGCC AGGAGCCAGT CATTCTGGAG CGCAAGCTAC CCTACCACAT GCGGCTGGAG 
CACGCGCTAA TGTCCTTCGC CATTCGACGC AGCAACAAGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spi ( NM_134293.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_599120.2 Drosophila melanogaster spitz (spi), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa16903
Clone ID Related Accession (Same CDS sequence)	NM_134292.3 , NM_134293.3 , NM_057561.6 , NM_001032110.2 , NM_134291.5
Accession Version	NM_134293.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spitz, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On May 8, 2012 this sequence version replaced NM_134293.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATTCCA CAATGAGTGT ACAACATGGG CTGGTTGCCC TGGTACTGAT CGGCTGTCTG 
GCACATCCGT GGCACGTCGA GGCCTGCTCC AGCCGTACGG TGCCCAAGCC GCGCTCTTCG 
ATCTCCTCGT CCATGTCCGG CACGGCATTG CCGCCGACCC AGGCTCCAGT CACTAGTAGC 
ACCACAATGC GCACCACCAC GACGACCACG CCCAGGCCCA ATATTACATT CCCCACATAC 
AAATGTCCGG AAACCTTCGA TGCCTGGTAC TGTTTGAACG ATGCCCATTG CTTTGCGGTG 
AAGATAGCCG ATCTACCGGT TTACAGCTGC GAGTGCGCGA TTGGCTTTAT GGGACAGCGA 
TGCGAATACA AGGAGATCGA CAATACTTAC CTGCCCAAGA GGCCGCGTCC GATGTTGGAG 
AAGGCGAGCA TTGCCAGTGG AGCCATGTGT GCCCTGGTAT TTATGCTGTT TGTCTGCCTG 
GCCTTCTATT TGCGCTTCGA GCAGCGGGCT GCCAAGAAGG CCTACGAACT GGAGCAGGAA 
CTGCAGCAGG AATACGACGA TGACGACGGC CAGTGCGAGT GCTGCCGCAA CCGGTGCTGT 
CCAGATGGCC AGGAGCCAGT CATTCTGGAG CGCAAGCTAC CCTACCACAT GCGGCTGGAG 
CACGCGCTAA TGTCCTTCGC CATTCGACGC AGCAACAAGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_599120.2
CDS	537..1241
Translation	MHSTMSVQHGLVALVLIGCLAHPWHVEACSSRTVPKPRSSISSSMSGTALPPTQAPVTSSTTMRTTTTTTPRPNITFPTYKCPETFDAWYCLNDAHCFAVKIADLPVYSCECAIGFMGQRCEYKEIDNTYLPKRPRPMLEKASIASGAMCALVFMLFVCLAFYLRFEQRAAKKAYELEQELQQEYDDDDGQCECCRNRCCPDGQEPVILERKLPYHMRLEHALMSFAIRRSNKL

Target ORF information:

RefSeq Version	NM_134293.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spitz (spi), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134293.3

ORF Insert Sequence:

ATGCATTCCA CAATGAGTGT ACAACATGGG CTGGTTGCCC TGGTACTGAT CGGCTGTCTG 
GCACATCCGT GGCACGTCGA GGCCTGCTCC AGCCGTACGG TGCCCAAGCC GCGCTCTTCG 
ATCTCCTCGT CCATGTCCGG CACGGCATTG CCGCCGACCC AGGCTCCAGT CACTAGTAGC 
ACCACAATGC GCACCACCAC GACGACCACG CCCAGGCCCA ATATTACATT CCCCACATAC 
AAATGTCCGG AAACCTTCGA TGCCTGGTAC TGTTTGAACG ATGCCCATTG CTTTGCGGTG 
AAGATAGCCG ATCTACCGGT TTACAGCTGC GAGTGCGCGA TTGGCTTTAT GGGACAGCGA 
TGCGAATACA AGGAGATCGA CAATACTTAC CTGCCCAAGA GGCCGCGTCC GATGTTGGAG 
AAGGCGAGCA TTGCCAGTGG AGCCATGTGT GCCCTGGTAT TTATGCTGTT TGTCTGCCTG 
GCCTTCTATT TGCGCTTCGA GCAGCGGGCT GCCAAGAAGG CCTACGAACT GGAGCAGGAA 
CTGCAGCAGG AATACGACGA TGACGACGGC CAGTGCGAGT GCTGCCGCAA CCGGTGCTGT 
CCAGATGGCC AGGAGCCAGT CATTCTGGAG CGCAAGCTAC CCTACCACAT GCGGCTGGAG 
CACGCGCTAA TGTCCTTCGC CATTCGACGC AGCAACAAGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spi ( NM_057561.6 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476909.2 Drosophila melanogaster spitz (spi), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa16903
Clone ID Related Accession (Same CDS sequence)	NM_134292.3 , NM_134293.3 , NM_057561.6 , NM_001032110.2 , NM_134291.5
Accession Version	NM_057561.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spitz, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057561.5. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATTCCA CAATGAGTGT ACAACATGGG CTGGTTGCCC TGGTACTGAT CGGCTGTCTG 
GCACATCCGT GGCACGTCGA GGCCTGCTCC AGCCGTACGG TGCCCAAGCC GCGCTCTTCG 
ATCTCCTCGT CCATGTCCGG CACGGCATTG CCGCCGACCC AGGCTCCAGT CACTAGTAGC 
ACCACAATGC GCACCACCAC GACGACCACG CCCAGGCCCA ATATTACATT CCCCACATAC 
AAATGTCCGG AAACCTTCGA TGCCTGGTAC TGTTTGAACG ATGCCCATTG CTTTGCGGTG 
AAGATAGCCG ATCTACCGGT TTACAGCTGC GAGTGCGCGA TTGGCTTTAT GGGACAGCGA 
TGCGAATACA AGGAGATCGA CAATACTTAC CTGCCCAAGA GGCCGCGTCC GATGTTGGAG 
AAGGCGAGCA TTGCCAGTGG AGCCATGTGT GCCCTGGTAT TTATGCTGTT TGTCTGCCTG 
GCCTTCTATT TGCGCTTCGA GCAGCGGGCT GCCAAGAAGG CCTACGAACT GGAGCAGGAA 
CTGCAGCAGG AATACGACGA TGACGACGGC CAGTGCGAGT GCTGCCGCAA CCGGTGCTGT 
CCAGATGGCC AGGAGCCAGT CATTCTGGAG CGCAAGCTAC CCTACCACAT GCGGCTGGAG 
CACGCGCTAA TGTCCTTCGC CATTCGACGC AGCAACAAGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476909.2
CDS	360..1064
Translation	MHSTMSVQHGLVALVLIGCLAHPWHVEACSSRTVPKPRSSISSSMSGTALPPTQAPVTSSTTMRTTTTTTPRPNITFPTYKCPETFDAWYCLNDAHCFAVKIADLPVYSCECAIGFMGQRCEYKEIDNTYLPKRPRPMLEKASIASGAMCALVFMLFVCLAFYLRFEQRAAKKAYELEQELQQEYDDDDGQCECCRNRCCPDGQEPVILERKLPYHMRLEHALMSFAIRRSNKL

Target ORF information:

RefSeq Version	NM_057561.6
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spitz (spi), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057561.6

ORF Insert Sequence:

ATGCATTCCA CAATGAGTGT ACAACATGGG CTGGTTGCCC TGGTACTGAT CGGCTGTCTG 
GCACATCCGT GGCACGTCGA GGCCTGCTCC AGCCGTACGG TGCCCAAGCC GCGCTCTTCG 
ATCTCCTCGT CCATGTCCGG CACGGCATTG CCGCCGACCC AGGCTCCAGT CACTAGTAGC 
ACCACAATGC GCACCACCAC GACGACCACG CCCAGGCCCA ATATTACATT CCCCACATAC 
AAATGTCCGG AAACCTTCGA TGCCTGGTAC TGTTTGAACG ATGCCCATTG CTTTGCGGTG 
AAGATAGCCG ATCTACCGGT TTACAGCTGC GAGTGCGCGA TTGGCTTTAT GGGACAGCGA 
TGCGAATACA AGGAGATCGA CAATACTTAC CTGCCCAAGA GGCCGCGTCC GATGTTGGAG 
AAGGCGAGCA TTGCCAGTGG AGCCATGTGT GCCCTGGTAT TTATGCTGTT TGTCTGCCTG 
GCCTTCTATT TGCGCTTCGA GCAGCGGGCT GCCAAGAAGG CCTACGAACT GGAGCAGGAA 
CTGCAGCAGG AATACGACGA TGACGACGGC CAGTGCGAGT GCTGCCGCAA CCGGTGCTGT 
CCAGATGGCC AGGAGCCAGT CATTCTGGAG CGCAAGCTAC CCTACCACAT GCGGCTGGAG 
CACGCGCTAA TGTCCTTCGC CATTCGACGC AGCAACAAGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spi ( NM_001032110.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001027281.1 Drosophila melanogaster spitz (spi), transcript variant G, mRNA.

Price & Availability↑

CloneID	OFa16903
Clone ID Related Accession (Same CDS sequence)	NM_134292.3 , NM_134293.3 , NM_057561.6 , NM_001032110.2 , NM_134291.5
Accession Version	NM_001032110.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spitz, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On May 8, 2012 this sequence version replaced NM_001032110.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATTCCA CAATGAGTGT ACAACATGGG CTGGTTGCCC TGGTACTGAT CGGCTGTCTG 
GCACATCCGT GGCACGTCGA GGCCTGCTCC AGCCGTACGG TGCCCAAGCC GCGCTCTTCG 
ATCTCCTCGT CCATGTCCGG CACGGCATTG CCGCCGACCC AGGCTCCAGT CACTAGTAGC 
ACCACAATGC GCACCACCAC GACGACCACG CCCAGGCCCA ATATTACATT CCCCACATAC 
AAATGTCCGG AAACCTTCGA TGCCTGGTAC TGTTTGAACG ATGCCCATTG CTTTGCGGTG 
AAGATAGCCG ATCTACCGGT TTACAGCTGC GAGTGCGCGA TTGGCTTTAT GGGACAGCGA 
TGCGAATACA AGGAGATCGA CAATACTTAC CTGCCCAAGA GGCCGCGTCC GATGTTGGAG 
AAGGCGAGCA TTGCCAGTGG AGCCATGTGT GCCCTGGTAT TTATGCTGTT TGTCTGCCTG 
GCCTTCTATT TGCGCTTCGA GCAGCGGGCT GCCAAGAAGG CCTACGAACT GGAGCAGGAA 
CTGCAGCAGG AATACGACGA TGACGACGGC CAGTGCGAGT GCTGCCGCAA CCGGTGCTGT 
CCAGATGGCC AGGAGCCAGT CATTCTGGAG CGCAAGCTAC CCTACCACAT GCGGCTGGAG 
CACGCGCTAA TGTCCTTCGC CATTCGACGC AGCAACAAGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001027281.1
CDS	479..1183
Translation	MHSTMSVQHGLVALVLIGCLAHPWHVEACSSRTVPKPRSSISSSMSGTALPPTQAPVTSSTTMRTTTTTTPRPNITFPTYKCPETFDAWYCLNDAHCFAVKIADLPVYSCECAIGFMGQRCEYKEIDNTYLPKRPRPMLEKASIASGAMCALVFMLFVCLAFYLRFEQRAAKKAYELEQELQQEYDDDDGQCECCRNRCCPDGQEPVILERKLPYHMRLEHALMSFAIRRSNKL

Target ORF information:

RefSeq Version	NM_001032110.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spitz (spi), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001032110.2

ORF Insert Sequence:

ATGCATTCCA CAATGAGTGT ACAACATGGG CTGGTTGCCC TGGTACTGAT CGGCTGTCTG 
GCACATCCGT GGCACGTCGA GGCCTGCTCC AGCCGTACGG TGCCCAAGCC GCGCTCTTCG 
ATCTCCTCGT CCATGTCCGG CACGGCATTG CCGCCGACCC AGGCTCCAGT CACTAGTAGC 
ACCACAATGC GCACCACCAC GACGACCACG CCCAGGCCCA ATATTACATT CCCCACATAC 
AAATGTCCGG AAACCTTCGA TGCCTGGTAC TGTTTGAACG ATGCCCATTG CTTTGCGGTG 
AAGATAGCCG ATCTACCGGT TTACAGCTGC GAGTGCGCGA TTGGCTTTAT GGGACAGCGA 
TGCGAATACA AGGAGATCGA CAATACTTAC CTGCCCAAGA GGCCGCGTCC GATGTTGGAG 
AAGGCGAGCA TTGCCAGTGG AGCCATGTGT GCCCTGGTAT TTATGCTGTT TGTCTGCCTG 
GCCTTCTATT TGCGCTTCGA GCAGCGGGCT GCCAAGAAGG CCTACGAACT GGAGCAGGAA 
CTGCAGCAGG AATACGACGA TGACGACGGC CAGTGCGAGT GCTGCCGCAA CCGGTGCTGT 
CCAGATGGCC AGGAGCCAGT CATTCTGGAG CGCAAGCTAC CCTACCACAT GCGGCTGGAG 
CACGCGCTAA TGTCCTTCGC CATTCGACGC AGCAACAAGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spi ( NM_134291.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_599118.2 Drosophila melanogaster spitz (spi), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa16903
Clone ID Related Accession (Same CDS sequence)	NM_134292.3 , NM_134293.3 , NM_057561.6 , NM_001032110.2 , NM_134291.5
Accession Version	NM_134291.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spitz, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_134291.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATTCCA CAATGAGTGT ACAACATGGG CTGGTTGCCC TGGTACTGAT CGGCTGTCTG 
GCACATCCGT GGCACGTCGA GGCCTGCTCC AGCCGTACGG TGCCCAAGCC GCGCTCTTCG 
ATCTCCTCGT CCATGTCCGG CACGGCATTG CCGCCGACCC AGGCTCCAGT CACTAGTAGC 
ACCACAATGC GCACCACCAC GACGACCACG CCCAGGCCCA ATATTACATT CCCCACATAC 
AAATGTCCGG AAACCTTCGA TGCCTGGTAC TGTTTGAACG ATGCCCATTG CTTTGCGGTG 
AAGATAGCCG ATCTACCGGT TTACAGCTGC GAGTGCGCGA TTGGCTTTAT GGGACAGCGA 
TGCGAATACA AGGAGATCGA CAATACTTAC CTGCCCAAGA GGCCGCGTCC GATGTTGGAG 
AAGGCGAGCA TTGCCAGTGG AGCCATGTGT GCCCTGGTAT TTATGCTGTT TGTCTGCCTG 
GCCTTCTATT TGCGCTTCGA GCAGCGGGCT GCCAAGAAGG CCTACGAACT GGAGCAGGAA 
CTGCAGCAGG AATACGACGA TGACGACGGC CAGTGCGAGT GCTGCCGCAA CCGGTGCTGT 
CCAGATGGCC AGGAGCCAGT CATTCTGGAG CGCAAGCTAC CCTACCACAT GCGGCTGGAG 
CACGCGCTAA TGTCCTTCGC CATTCGACGC AGCAACAAGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_599118.2
CDS	556..1260
Translation	MHSTMSVQHGLVALVLIGCLAHPWHVEACSSRTVPKPRSSISSSMSGTALPPTQAPVTSSTTMRTTTTTTPRPNITFPTYKCPETFDAWYCLNDAHCFAVKIADLPVYSCECAIGFMGQRCEYKEIDNTYLPKRPRPMLEKASIASGAMCALVFMLFVCLAFYLRFEQRAAKKAYELEQELQQEYDDDDGQCECCRNRCCPDGQEPVILERKLPYHMRLEHALMSFAIRRSNKL

Target ORF information:

RefSeq Version	NM_134291.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spitz (spi), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134291.5

ORF Insert Sequence:

ATGCATTCCA CAATGAGTGT ACAACATGGG CTGGTTGCCC TGGTACTGAT CGGCTGTCTG 
GCACATCCGT GGCACGTCGA GGCCTGCTCC AGCCGTACGG TGCCCAAGCC GCGCTCTTCG 
ATCTCCTCGT CCATGTCCGG CACGGCATTG CCGCCGACCC AGGCTCCAGT CACTAGTAGC 
ACCACAATGC GCACCACCAC GACGACCACG CCCAGGCCCA ATATTACATT CCCCACATAC 
AAATGTCCGG AAACCTTCGA TGCCTGGTAC TGTTTGAACG ATGCCCATTG CTTTGCGGTG 
AAGATAGCCG ATCTACCGGT TTACAGCTGC GAGTGCGCGA TTGGCTTTAT GGGACAGCGA 
TGCGAATACA AGGAGATCGA CAATACTTAC CTGCCCAAGA GGCCGCGTCC GATGTTGGAG 
AAGGCGAGCA TTGCCAGTGG AGCCATGTGT GCCCTGGTAT TTATGCTGTT TGTCTGCCTG 
GCCTTCTATT TGCGCTTCGA GCAGCGGGCT GCCAAGAAGG CCTACGAACT GGAGCAGGAA 
CTGCAGCAGG AATACGACGA TGACGACGGC CAGTGCGAGT GCTGCCGCAA CCGGTGCTGT 
CCAGATGGCC AGGAGCCAGT CATTCTGGAG CGCAAGCTAC CCTACCACAT GCGGCTGGAG 
CACGCGCTAA TGTCCTTCGC CATTCGACGC AGCAACAAGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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spi cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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