Nak cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nak
Entrez Gene ID	35154
Full Name	Numb-associated kinase
Synonyms	CG10637,Dmel\CG10637,nak
General protein information	Preferred Names Numb-associated kinase Names CG10637 gene product from transcript CG10637-RD CG10637-PA CG10637-PC CG10637-PD CG10637-PE Nak-PA Nak-PC Nak-PD Nak-PE
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 37B7-37B7

mRNA and Protein(s)

mRNA	Protein	Name
NM_057818.4	NP_477166.2	Numb-associated kinase, isoform D
NM_206003.2	NP_995725.1	Numb-associated kinase, isoform C
NM_001299146.1	NP_001286075.1	Numb-associated kinase, isoform E
NM_057817.4	NP_477165.1	Numb-associated kinase, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nak interacts genetically with components of the endocytic pathway, colocalizes with clathrin puncta, and is required for dendritic localization of clathrin puncta.
Title: Nak regulates localization of clathrin sites in higher-order dendrites to promote local dendrite growth.

The following Nak gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nak cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa16024	NM_206003.2 Latest version!	Drosophila melanogaster Numb-associated kinase (Nak), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa21682	NM_001299146.1 Latest version!	Drosophila melanogaster Numb-associated kinase (Nak), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa16022	NM_057818.4 Latest version!	Drosophila melanogaster Numb-associated kinase (Nak), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OFa16022	NM_057817.4 Latest version!	Drosophila melanogaster Numb-associated kinase (Nak), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nak ( NM_206003.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995725.1 Drosophila melanogaster Numb-associated kinase (Nak), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa16024
Clone ID Related Accession (Same CDS sequence)	NM_206003.2
Accession Version	NM_206003.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Numb-associated kinase, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_206003.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGAAAA TCCTGTCTAA GTTCGAGCGC AATGAGAACT CTCGACTGGA GAATCCGCCC 
AACAGCAGCA GCTCGTCGTC CTCGAACGAG AAGAACAGCT TCGTGGGCAA GGTCTTCACA 
GTGGGACGCG TCACGGTCAC CGTGGAGGAT GTCCTGGCAG AAGGTGGTTT CGCCATGGTC 
TTTTTGGCAA GGGGCAACGG CGGAGGCAGT TCCTCGGCGA CCAAGTACGC CCTGAAGCGC 
ATGTACGTCA ACAATGAGCA CGATCTGAAC GTAGCCAAGC GGGAGATACA GATTGCGAGC 
AACCTGAGTG GGCACAAAAA CATCATTGGT TATGTGGACT CCAGCATCAC GCCCACGGGA 
AACGGGGTGT GCGAGGTGCT ACTCCTGATG CCCTATTGCA AGCATCACAT GCTGGCCATG 
ATGAATGCGA GATTGCACGT TGGCTTCACG GAGCCAGAGG TGCTCAACAT CTTCTGTGAT 
ATTGCCGAGG CTGTCTCTCG ATTGCACTAT TGTCAGACTC CGATTATCCA TCGGGATCTT 
AAGGTGGAGA ACATTCTGCA AACGGACGCG GGCAACTTCG TACTCTGCGA CTTCGGATCG 
GCAACGGCCA AGACGTTAAA TCCTCAACAG CACGGAGTCA CCGTGGTGCA GGAAGAGATC 
CAAAAGTACA CCACTCTATC TTATCGGGCT CCCGAGATGA TCGACCTGTA CGGGGGCAAG 
AGCATAACCA CAAAGGCGGA CATCTGGGCA CTGGGTTGCA TGCTCTATAA GCTGTGCTTC 
TTTTCCTTGC CATTTGGGGA GAGCACTTTG GCTATCCAGA ATGGACAGTT TTCCATTCCA 
GACTCCTCAA AGTACTCGAA GGGCATGCAC CAACTGATTA AATATATGTT GGAACCCGAT 
ATGGAGAAGC GACCAAACAT TTGGCAAGTT TGCGAGGTGG CCTTTCGACT GGCGGGCAAG 
GATAATCCCG TTCAGAATCT CCATAAAACT CCCATTCCAA ACTTCGACCT ACTGGTAGTG 
CCTCCGTTTG AATCGGAAGC CAAGAGGATC AGTGCAGCGG CCTCCAAGGC GGCGAAGGCT 
CAGAATGTGT CAGTTGTGGA GGCGGGAACC AGTGTAGCTC CTCGGTCTCG TCCTAAGGGT 
TCCTCATCGG TACATGGAGG AAATCCATTG GGTCTCGGTT TGCCTCCCAG TCCCTCGCCA 
AGACAGAATA TCACTTCTCC ACAACCCCAA CCTCAACCTG TGGTCGAGCA GTTCCAGGCC 
AACTTCCCAG CTATGCCACC TGCTGTTCCT CCACCTCCTC AAGCTGTTAC TCCGGCAGCT 
CCTGCTGCCA GTAAACTCTT CGAGTCAAGT GGCTATCCAG ATCCATTCAA TGAGCCAGCT 
GCAGCAGTAA TTCCTACTGA ATTTCTACCA GCAGCAGAAG AACCTAACAA GGAGGAAGTG 
CCTCAGGACT TAAACGTCAT TGCAGGCACG CCCACCAAGA GCATGTTGAC ACCGCCCAAG 
CCAAGCGGAG GAGGTGGTCA TAGGCGTAAC GTAAGCGACA CCTCGGCCTT CAATAAAACT 
TTTGCCAATG AGACAAGTCA GTTTCTGGCT CCGTTTAACA ATAACTTGGC TGCCATGGGC 
GACGGTCAGC AGACTTTGGC TAGTGCTGCC TCGAATCCCG GGATATATGC ATCTTCAACG 
GCAAACTCAG CCGCGGTATC CATTAGCGAA TTAATGAAAC CAGGACAAAC GGCGGTGGAA 
AAGCGAGTTG AAGCCTGGAA TCCGTTCGAA GAGGAGCAGC CTTTCAGCCA GATGACTGAG 
GATCACATCT TTGAGGCGGA GTTCGACAAG ATACGACAGA GAGGCAGTCA AGGAAGCATC 
ACAGCAAAAT CGGCATCCAC AACTTCCACG CTAACACCAA CGGAGCAGAT TCCCTCTGCG 
GTTGTGGCTC CTGTTACAGC TGCTCCTGGT GCAGTGCCTC CTCCGCCGAT TGCTGCCGCT 
GTGTCTCCTT ACCCGTCGCA GGTCTCCGAG GATCCATTCG GATCGGCGCC ATTCAGTTTG 
CCACCCGGAC TGCGCGAGAA GGCGAGCTCG CTGCGCAAAA CCGGAGGTAA AGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995725.1
CDS	307..2403
Translation	MKKILSKFERNENSRLENPPNSSSSSSSNEKNSFVGKVFTVGRVTVTVEDVLAEGGFAMVFLARGNGGGSSSATKYALKRMYVNNEHDLNVAKREIQIASNLSGHKNIIGYVDSSITPTGNGVCEVLLLMPYCKHHMLAMMNARLHVGFTEPEVLNIFCDIAEAVSRLHYCQTPIIHRDLKVENILQTDAGNFVLCDFGSATAKTLNPQQHGVTVVQEEIQKYTTLSYRAPEMIDLYGGKSITTKADIWALGCMLYKLCFFSLPFGESTLAIQNGQFSIPDSSKYSKGMHQLIKYMLEPDMEKRPNIWQVCEVAFRLAGKDNPVQNLHKTPIPNFDLLVVPPFESEAKRISAAASKAAKAQNVSVVEAGTSVAPRSRPKGSSSVHGGNPLGLGLPPSPSPRQNITSPQPQPQPVVEQFQANFPAMPPAVPPPPQAVTPAAPAASKLFESSGYPDPFNEPAAAVIPTEFLPAAEEPNKEEVPQDLNVIAGTPTKSMLTPPKPSGGGGHRRNVSDTSAFNKTFANETSQFLAPFNNNLAAMGDGQQTLASAASNPGIYASSTANSAAVSISELMKPGQTAVEKRVEAWNPFEEEQPFSQMTEDHIFEAEFDKIRQRGSQGSITAKSASTTSTLTPTEQIPSAVVAPVTAAPGAVPPPPIAAAVSPYPSQVSEDPFGSAPFSLPPGLREKASSLRKTGGKA

Target ORF information:

RefSeq Version	NM_206003.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Numb-associated kinase (Nak), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206003.2

ORF Insert Sequence:

ATGAAGAAAA TCCTGTCTAA GTTCGAGCGC AATGAGAACT CTCGACTGGA GAATCCGCCC 
AACAGCAGCA GCTCGTCGTC CTCGAACGAG AAGAACAGCT TCGTGGGCAA GGTCTTCACA 
GTGGGACGCG TCACGGTCAC CGTGGAGGAT GTCCTGGCAG AAGGTGGTTT CGCCATGGTC 
TTTTTGGCAA GGGGCAACGG CGGAGGCAGT TCCTCGGCGA CCAAGTACGC CCTGAAGCGC 
ATGTACGTCA ACAATGAGCA CGATCTGAAC GTAGCCAAGC GGGAGATACA GATTGCGAGC 
AACCTGAGTG GGCACAAAAA CATCATTGGT TATGTGGACT CCAGCATCAC GCCCACGGGA 
AACGGGGTGT GCGAGGTGCT ACTCCTGATG CCCTATTGCA AGCATCACAT GCTGGCCATG 
ATGAATGCGA GATTGCACGT TGGCTTCACG GAGCCAGAGG TGCTCAACAT CTTCTGTGAT 
ATTGCCGAGG CTGTCTCTCG ATTGCACTAT TGTCAGACTC CGATTATCCA TCGGGATCTT 
AAGGTGGAGA ACATTCTGCA AACGGACGCG GGCAACTTCG TACTCTGCGA CTTCGGATCG 
GCAACGGCCA AGACGTTAAA TCCTCAACAG CACGGAGTCA CCGTGGTGCA GGAAGAGATC 
CAAAAGTACA CCACTCTATC TTATCGGGCT CCCGAGATGA TCGACCTGTA CGGGGGCAAG 
AGCATAACCA CAAAGGCGGA CATCTGGGCA CTGGGTTGCA TGCTCTATAA GCTGTGCTTC 
TTTTCCTTGC CATTTGGGGA GAGCACTTTG GCTATCCAGA ATGGACAGTT TTCCATTCCA 
GACTCCTCAA AGTACTCGAA GGGCATGCAC CAACTGATTA AATATATGTT GGAACCCGAT 
ATGGAGAAGC GACCAAACAT TTGGCAAGTT TGCGAGGTGG CCTTTCGACT GGCGGGCAAG 
GATAATCCCG TTCAGAATCT CCATAAAACT CCCATTCCAA ACTTCGACCT ACTGGTAGTG 
CCTCCGTTTG AATCGGAAGC CAAGAGGATC AGTGCAGCGG CCTCCAAGGC GGCGAAGGCT 
CAGAATGTGT CAGTTGTGGA GGCGGGAACC AGTGTAGCTC CTCGGTCTCG TCCTAAGGGT 
TCCTCATCGG TACATGGAGG AAATCCATTG GGTCTCGGTT TGCCTCCCAG TCCCTCGCCA 
AGACAGAATA TCACTTCTCC ACAACCCCAA CCTCAACCTG TGGTCGAGCA GTTCCAGGCC 
AACTTCCCAG CTATGCCACC TGCTGTTCCT CCACCTCCTC AAGCTGTTAC TCCGGCAGCT 
CCTGCTGCCA GTAAACTCTT CGAGTCAAGT GGCTATCCAG ATCCATTCAA TGAGCCAGCT 
GCAGCAGTAA TTCCTACTGA ATTTCTACCA GCAGCAGAAG AACCTAACAA GGAGGAAGTG 
CCTCAGGACT TAAACGTCAT TGCAGGCACG CCCACCAAGA GCATGTTGAC ACCGCCCAAG 
CCAAGCGGAG GAGGTGGTCA TAGGCGTAAC GTAAGCGACA CCTCGGCCTT CAATAAAACT 
TTTGCCAATG AGACAAGTCA GTTTCTGGCT CCGTTTAACA ATAACTTGGC TGCCATGGGC 
GACGGTCAGC AGACTTTGGC TAGTGCTGCC TCGAATCCCG GGATATATGC ATCTTCAACG 
GCAAACTCAG CCGCGGTATC CATTAGCGAA TTAATGAAAC CAGGACAAAC GGCGGTGGAA 
AAGCGAGTTG AAGCCTGGAA TCCGTTCGAA GAGGAGCAGC CTTTCAGCCA GATGACTGAG 
GATCACATCT TTGAGGCGGA GTTCGACAAG ATACGACAGA GAGGCAGTCA AGGAAGCATC 
ACAGCAAAAT CGGCATCCAC AACTTCCACG CTAACACCAA CGGAGCAGAT TCCCTCTGCG 
GTTGTGGCTC CTGTTACAGC TGCTCCTGGT GCAGTGCCTC CTCCGCCGAT TGCTGCCGCT 
GTGTCTCCTT ACCCGTCGCA GGTCTCCGAG GATCCATTCG GATCGGCGCC ATTCAGTTTG 
CCACCCGGAC TGCGCGAGAA GGCGAGCTCG CTGCGCAAAA CCGGAGGTAA AGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nak ( NM_001299146.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286075.1 Drosophila melanogaster Numb-associated kinase (Nak), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa21682
Clone ID Related Accession (Same CDS sequence)	NM_001299146.1
Accession Version	NM_001299146.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Numb-associated kinase, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGAAAA TCCTGTCTAA GTTCGAGCGC AATGAGAACT CTCGACTGGA GAATCCGCCC 
AACAGCAGCA GCTCGTCGTC CTCGAACGAG AAGAACAGCT TCGTGGGCAA GGTCTTCACA 
GTGGGACGCG TCACGGTCAC CGTGGAGGAT GTCCTGGCAG AAGGTGGTTT CGCCATGGTC 
TTTTTGGCAA GGGGCAACGG CGGAGGCAGT TCCTCGGCGA CCAAGTACGC CCTGAAGCGC 
ATGTACGTCA ACAATGAGCA CGATCTGAAC GTAGCCAAGC GGGAGATACA GATTGCGAGC 
AACCTGAGTG GGCACAAAAA CATCATTGGT TATGTGGACT CCAGCATCAC GCCCACGGGA 
AACGGGGTGT GCGAGGTGCT ACTCCTGATG CCCTATTGCA AGCATCACAT GCTGGCCATG 
ATGAATGCGA GATTGCACGT TGGCTTCACG GAGCCAGAGG TGCTCAACAT CTTCTGTGAT 
ATTGCCGAGG CTGTCTCTCG ATTGCACTAT TGTCAGACTC CGATTATCCA TCGGGATCTT 
AAGGTGGAGA ACATTCTGCA AACGGACGCG GGCAACTTCG TACTCTGCGA CTTCGGATCG 
GCAACGGCCA AGACGTTAAA TCCTCAACAG CACGGAGTCA CCGTGGTGCA GGAAGAGATC 
CAAAAGTACA CCACTCTATC TTATCGGGCT CCCGAGATGA TCGACCTGTA CGGGGGCAAG 
AGCATAACCA CAAAGGCGGA CATCTGGGCA CTGGGTTGCA TGCTCTATAA GCTGTGCTTC 
TTTTCCTTGC CATTTGGGGA GAGCACTTTG GCTATCCAGA ATGGACAGTT TTCCATTCCA 
GACTCCTCAA AGTACTCGAA GGGCATGCAC CAACTGATTA AATATATGTT GGAACCCGAT 
ATGGAGAAGC GACCAAACAT TTGGCAAGTT TGCGAGGTGG CCTTTCGACT GGCGGGCAAG 
GATAATCCCG TTCAGAATCT CCATAAAACT CCCATTCCAA ACTTCGACCT ACTGGTAGTG 
CCTCCGTTTG AATCGGAAGC CAAGAGGATC AGTGCAGCGG CCTCCAAGGC GGCGAAGGCT 
CAGAATGTGT CAGTTGTGGA GGCGGGAACC AGTGTAGCTC CTCGGTCTCG TCCTAAGGGT 
TCCTCATCGG TACATGGAGG AAATCCATTG GGTCTCGGTT TGCCTCCCAG TCCCTCGCCA 
AGACAGAATA TCACTTCTCC ACAACCCCAA CCTCAACCTG TGGTCGAGCA GTTCCAGGCC 
AACTTCCCAG CTATGCCACC TGCTGTTCCT CCACCTCCTC AAGCTGTTAC TCCGGCAGCT 
CCTGCTGCCA GTAAACTCTT CGAGTCAAGT GGCTATCCAG ATCCATTCAA TGAGCCAGCT 
GCAGCAGTAA TTCCTACTGA ATTTCTACCA GCAGCAGAAG AACCTAACAA GGAGGAAGTG 
CCTCAGGACT TAAACGTCAT TGCAGGCACG CCCACCAAGA GCATGTTGAC ACCGCCCAAG 
CCAAGCGGAG GAGGTGGTCA TAGGCGTAAC GTAAGCGACA CCTCGGCCTT CAATAAAACT 
TTTGCCAATG AGACAAGTCA GTTTCTGGCT CCGTTTAACA ATAACTTGGC TGCCATGGGC 
GACGGTCAGC AGACTTTGGC TAGTGCTGCC TCGAATCCCG GGATATATGC ATCTTCAACG 
GCAAACTCAG CCGCGGTATC CATTAGCGAA TTAATGAAAC CAGGACAAAC GGCGGTGGAA 
AAGCGAGTTG AAGCCTGGAA TCCGTTCGAA GAGGAGCAGC CTTTCAGCCA GATGACTGAG 
GATCACATCT TTGAGGCGGA GTTCGACAAG ATACGACAGA GAGGCAGTCA AGGAAGCATC 
ACAGCAAAAT CGGCATCCAC AACTTCCACG CTAACACCAA CGGAGCAGAT TCCCTCTGCG 
GTTGTGGCTC CTGTTACAGC TGCTCCTGGT GCAGTGCCTC CTCCGCCGAT TGCTGCCGCT 
GTGTCTCCTT ACCCGTCGCA GGTCTCCGAG GATCCATTCG GATCGGCGCC ATTCAGTTTG 
CCACCCGGAC TGCGCGAGAA GGCGAGCTCG CTGCGCAAAA CCGGAGAAAG TGAAAGTGAT 
TTGAACGTAG ACGTAGACGT AGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286075.1
CDS	330..2456
Translation	MKKILSKFERNENSRLENPPNSSSSSSSNEKNSFVGKVFTVGRVTVTVEDVLAEGGFAMVFLARGNGGGSSSATKYALKRMYVNNEHDLNVAKREIQIASNLSGHKNIIGYVDSSITPTGNGVCEVLLLMPYCKHHMLAMMNARLHVGFTEPEVLNIFCDIAEAVSRLHYCQTPIIHRDLKVENILQTDAGNFVLCDFGSATAKTLNPQQHGVTVVQEEIQKYTTLSYRAPEMIDLYGGKSITTKADIWALGCMLYKLCFFSLPFGESTLAIQNGQFSIPDSSKYSKGMHQLIKYMLEPDMEKRPNIWQVCEVAFRLAGKDNPVQNLHKTPIPNFDLLVVPPFESEAKRISAAASKAAKAQNVSVVEAGTSVAPRSRPKGSSSVHGGNPLGLGLPPSPSPRQNITSPQPQPQPVVEQFQANFPAMPPAVPPPPQAVTPAAPAASKLFESSGYPDPFNEPAAAVIPTEFLPAAEEPNKEEVPQDLNVIAGTPTKSMLTPPKPSGGGGHRRNVSDTSAFNKTFANETSQFLAPFNNNLAAMGDGQQTLASAASNPGIYASSTANSAAVSISELMKPGQTAVEKRVEAWNPFEEEQPFSQMTEDHIFEAEFDKIRQRGSQGSITAKSASTTSTLTPTEQIPSAVVAPVTAAPGAVPPPPIAAAVSPYPSQVSEDPFGSAPFSLPPGLREKASSLRKTGESESDLNVDVDVV

Target ORF information:

RefSeq Version	NM_001299146.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Numb-associated kinase (Nak), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299146.1

ORF Insert Sequence:

ATGAAGAAAA TCCTGTCTAA GTTCGAGCGC AATGAGAACT CTCGACTGGA GAATCCGCCC 
AACAGCAGCA GCTCGTCGTC CTCGAACGAG AAGAACAGCT TCGTGGGCAA GGTCTTCACA 
GTGGGACGCG TCACGGTCAC CGTGGAGGAT GTCCTGGCAG AAGGTGGTTT CGCCATGGTC 
TTTTTGGCAA GGGGCAACGG CGGAGGCAGT TCCTCGGCGA CCAAGTACGC CCTGAAGCGC 
ATGTACGTCA ACAATGAGCA CGATCTGAAC GTAGCCAAGC GGGAGATACA GATTGCGAGC 
AACCTGAGTG GGCACAAAAA CATCATTGGT TATGTGGACT CCAGCATCAC GCCCACGGGA 
AACGGGGTGT GCGAGGTGCT ACTCCTGATG CCCTATTGCA AGCATCACAT GCTGGCCATG 
ATGAATGCGA GATTGCACGT TGGCTTCACG GAGCCAGAGG TGCTCAACAT CTTCTGTGAT 
ATTGCCGAGG CTGTCTCTCG ATTGCACTAT TGTCAGACTC CGATTATCCA TCGGGATCTT 
AAGGTGGAGA ACATTCTGCA AACGGACGCG GGCAACTTCG TACTCTGCGA CTTCGGATCG 
GCAACGGCCA AGACGTTAAA TCCTCAACAG CACGGAGTCA CCGTGGTGCA GGAAGAGATC 
CAAAAGTACA CCACTCTATC TTATCGGGCT CCCGAGATGA TCGACCTGTA CGGGGGCAAG 
AGCATAACCA CAAAGGCGGA CATCTGGGCA CTGGGTTGCA TGCTCTATAA GCTGTGCTTC 
TTTTCCTTGC CATTTGGGGA GAGCACTTTG GCTATCCAGA ATGGACAGTT TTCCATTCCA 
GACTCCTCAA AGTACTCGAA GGGCATGCAC CAACTGATTA AATATATGTT GGAACCCGAT 
ATGGAGAAGC GACCAAACAT TTGGCAAGTT TGCGAGGTGG CCTTTCGACT GGCGGGCAAG 
GATAATCCCG TTCAGAATCT CCATAAAACT CCCATTCCAA ACTTCGACCT ACTGGTAGTG 
CCTCCGTTTG AATCGGAAGC CAAGAGGATC AGTGCAGCGG CCTCCAAGGC GGCGAAGGCT 
CAGAATGTGT CAGTTGTGGA GGCGGGAACC AGTGTAGCTC CTCGGTCTCG TCCTAAGGGT 
TCCTCATCGG TACATGGAGG AAATCCATTG GGTCTCGGTT TGCCTCCCAG TCCCTCGCCA 
AGACAGAATA TCACTTCTCC ACAACCCCAA CCTCAACCTG TGGTCGAGCA GTTCCAGGCC 
AACTTCCCAG CTATGCCACC TGCTGTTCCT CCACCTCCTC AAGCTGTTAC TCCGGCAGCT 
CCTGCTGCCA GTAAACTCTT CGAGTCAAGT GGCTATCCAG ATCCATTCAA TGAGCCAGCT 
GCAGCAGTAA TTCCTACTGA ATTTCTACCA GCAGCAGAAG AACCTAACAA GGAGGAAGTG 
CCTCAGGACT TAAACGTCAT TGCAGGCACG CCCACCAAGA GCATGTTGAC ACCGCCCAAG 
CCAAGCGGAG GAGGTGGTCA TAGGCGTAAC GTAAGCGACA CCTCGGCCTT CAATAAAACT 
TTTGCCAATG AGACAAGTCA GTTTCTGGCT CCGTTTAACA ATAACTTGGC TGCCATGGGC 
GACGGTCAGC AGACTTTGGC TAGTGCTGCC TCGAATCCCG GGATATATGC ATCTTCAACG 
GCAAACTCAG CCGCGGTATC CATTAGCGAA TTAATGAAAC CAGGACAAAC GGCGGTGGAA 
AAGCGAGTTG AAGCCTGGAA TCCGTTCGAA GAGGAGCAGC CTTTCAGCCA GATGACTGAG 
GATCACATCT TTGAGGCGGA GTTCGACAAG ATACGACAGA GAGGCAGTCA AGGAAGCATC 
ACAGCAAAAT CGGCATCCAC AACTTCCACG CTAACACCAA CGGAGCAGAT TCCCTCTGCG 
GTTGTGGCTC CTGTTACAGC TGCTCCTGGT GCAGTGCCTC CTCCGCCGAT TGCTGCCGCT 
GTGTCTCCTT ACCCGTCGCA GGTCTCCGAG GATCCATTCG GATCGGCGCC ATTCAGTTTG 
CCACCCGGAC TGCGCGAGAA GGCGAGCTCG CTGCGCAAAA CCGGAGAAAG TGAAAGTGAT 
TTGAACGTAG ACGTAGACGT AGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nak ( NM_057818.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477166.2 Drosophila melanogaster Numb-associated kinase (Nak), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa16022
Clone ID Related Accession (Same CDS sequence)	NM_057818.4 , NM_057817.4
Accession Version	NM_057818.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4467bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Numb-associated kinase, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_057818.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGAAAA TCCTGTCTAA GTTCGAGCGC AATGAGAACT CTCGACTGGA GAATCCGCCC 
AACAGCAGCA GCTCGTCGTC CTCGAACGAG AAGAACAGCT TCGTGGGCAA GGTCTTCACA 
GTGGGACGCG TCACGGTCAC CGTGGAGGAT GTCCTGGCAG AAGGTGGTTT CGCCATGGTC 
TTTTTGGCAA GGGGCAACGG CGGAGGCAGT TCCTCGGCGA CCAAGTACGC CCTGAAGCGC 
ATGTACGTCA ACAATGAGCA CGATCTGAAC GTAGCCAAGC GGGAGATACA GATTGCGAGC 
AACCTGAGTG GGCACAAAAA CATCATTGGT TATGTGGACT CCAGCATCAC GCCCACGGGA 
AACGGGGTGT GCGAGGTGCT ACTCCTGATG CCCTATTGCA AGCATCACAT GCTGGCCATG 
ATGAATGCGA GATTGCACGT TGGCTTCACG GAGCCAGAGG TGCTCAACAT CTTCTGTGAT 
ATTGCCGAGG CTGTCTCTCG ATTGCACTAT TGTCAGACTC CGATTATCCA TCGGGATCTT 
AAGGTGGAGA ACATTCTGCA AACGGACGCG GGCAACTTCG TACTCTGCGA CTTCGGATCG 
GCAACGGCCA AGACGTTAAA TCCTCAACAG CACGGAGTCA CCGTGGTGCA GGAAGAGATC 
CAAAAGTACA CCACTCTATC TTATCGGGCT CCCGAGATGA TCGACCTGTA CGGGGGCAAG 
AGCATAACCA CAAAGGCGGA CATCTGGGCA CTGGGTTGCA TGCTCTATAA GCTGTGCTTC 
TTTTCCTTGC CATTTGGGGA GAGCACTTTG GCTATCCAGA ATGGACAGTT TTCCATTCCA 
GACTCCTCAA AGTACTCGAA GGGCATGCAC CAACTGATTA AATATATGTT GGAACCCGAT 
ATGGAGAAGC GACCAAACAT TTGGCAAGTT TGCGAGGTGG CCTTTCGACT GGCGGGCAAG 
GATAATCCCG TTCAGAATCT CCATAAAACT CCCATTCCAA ACTTCGACCT ACTGGTAGTG 
CCTCCGTTTG AATCGGAAGC CAAGAGGATC AGTGCAGCGG CCTCCAAGGC GGCGAAGGCT 
CAGAATGTGT CAGTTGTGGA GGCGGGAACC AGTGTAGCTC CTCGGTCTCG TCCTAAGGGT 
TCCTCATCGG TACATGGAGG AAATCCATTG GGTCTCGGTT TGCCTCCCAG TCCCTCGCCA 
AGACAGAATA TCACTTCTCC ACAACCCCAA CCTCAACCTG TGGTCGAGCA GTTCCAGGCC 
AACTTCCCAG CTATGCCACC TGCTGTTCCT CCACCTCCTC AAGCTGTTAC TCCGGCAGCT 
CCTGCTGCCA GTAAACTCTT CGAGTCAAGT GGCTATCCAG ATCCATTCAA TGAGCCAGCT 
GCAGCAGTAA TTCCTACTGA ATTTCTACCA GCAGCAGAAG AACCTAACAA GGAGGAAGTG 
CCTCAGGACT TAAACGTCAT TGCAGGCACG CCCACCAAGA GCATGTTGAC ACCGCCCAAG 
CCAAGCGGAG GAGGTGGTCA TAGGCGTAAC GTAAGCGACA CCTCGGCCTT CAATAAAACT 
TTTGCCAATG AGACAAGTCA GTTTCTGGCT CCGTTTAACA ATAACTTGGC TGCCATGGGC 
GACGGTCAGC AGACTTTGGC TAGTGCTGCC TCGAATCCCG GGATATATGC ATCTTCAACG 
GCAAACTCAG CCGCGGTATC CATTAGCGAA TTAATGAAAC CAGGACAAAC GGCGGTGGAA 
AAGCGAGTTG AAGCCTGGAA TCCGTTCGAA GAGGAGCAGC CTTTCAGCCA GATGACTGAG 
GATCACATCT TTGAGGCGGA GTTCGACAAG ATACGACAGA GAGGCAGTCA AGGAAGCATC 
ACAGCAAAAT CGGCATCCAC AACTTCCACG CTAACACCAA CGGAGCAGAT TCCCTCTGCG 
GTTGTGGCTC CTGTTACAGC TGCTCCTGGT GCAGTGCCTC CTCCGCCGAT TGCTGCCGCT 
GTGTCTCCTT ACCCGTCGCA GGTCTCCGAG GATCCATTCG GATCGGCGCC ATTCAGTTTG 
CCACCCGGAC TGCGCGAGAA GGCGAGCTCG CTGCGCAAAA CCGGAGCGAA ATTCATCGGC 
GTTTCATCTG GTGGAACAGG AGGCGGAGTG GCCACGCCTA TCGGCTTGGT AGCGGGAGGA 
GCAAACACCG CCACCTCTTC CAACTCATCC AAGTGGATGC TCTCCCCCAC CCTGGAGGTT 
TCCAGCGAAG AGAAGACCTC GCTTATCAGT AAACTCAAAA CAGACTCAGA ATGTGGAATT 
CCAGGCGCTG TTGGAGGAGC TGGAGAAGGT GTCTCTGGTT ATGTTAAGCT ACCTGTAGAA 
GATCGCAACA AGTACGAGAA GCTGCGCAGC AAAGATCAGC CGACTTCCGA TGACTCCGAT 
TCCGAGGCTT CGGAGTTCTT TCAACAAGAC AGCGATGAAG GAGGTGGGGG CATCTTTAGG 
CAAATGCAGA TGGTGACAAG CAATTTGCCG GAAACCATCC ATAAAATCCA ACAGGTTGCC 
TATCACAAGG TGGACAAGAC AGCTCATCTG CCAATTGTGA AGAAACTTCG CGATAAGACC 
AAGAAACCCA CAACAGCTGC CCATCAAGCG GCCCAACAAC TAAATCTCAT GGCTGCCGCG 
GTAGCTGCAC AAGCTGAAGA AGCTGCCAAG GCGGCGGAAT CCGACGGTGA TTCGATCGGA 
TCTGCCAGTG ACCTGCGGGC AGAGGATGAT GATCTGTTTG ACGAGAATCC TCGCCAGACA 
CAAGCCAAGC AACGTCGTCC TCTGCCAACA GAAATGGATG GAATAAGCGA GTCAGTGAAG 
ACCTGTAGCA GCTCTGCCTA CCATGCAGAA TGTGAAAGTG TGACCACCCA TGAAGACGAT 
GTGCGTAGTC GGGTGGTGGT GAAGGTGCGG ATGAGGAAGA AGGACCGTGC CCTTGTGACT 
GCCACTGGCG ACCCAAGTGC TCCGTCTGAA GAACTAAGTC CCACTTCGAG CGATCTCTTG 
CACAAGTTCG GCGATCGTCC GTTGCTGCTA GATGATGAAT TGGATTATGG TTCTGGAGAA 
AGCAAGAGTA GCACTGAATC TCCTGTGGCT CCAATTCTGC CAACAAATCC CGATGTGCAG 
GAATCAGAGG AGGATGTGTT CGCCCTGGCT CCATTTAAAT TACCAGTCGG AGTTCCTTTG 
AAACGAAAGA CTAAACCCAA AGCTAAGCCC AAGCCTTCGG AACCCGAGAT GTGGACTTCA 
ACGCCAGTTA AAAGCGCCGC TGTCGGAGAT CCTTCTACCA ACTTTGCCAG TTTTCCAGCG 
CAGCTCACGC CAACCAATAA TGTGGGGAAC TCTGCGCAGC TGGATGAGTT CAATGAGCCA 
ATTTTCAATC CCTTCCAGGA AAACGCACAG AACACGCAAT CAGGGGAAAC TAATCAATGC 
GACCTGTTCG GCTTGGAACC ATTTCCACAG GTAATTCGTA CAATCGAACA GAACCCACAG 
CACCACCAGA TCCACCAATT TCCCAGCCAC AACAACAATA ACAATGTGAT CAAAGTGCCC 
GCCTCCGTAT CTGTTGCTCT TCCTGCCTCC GTTCCTGTAT CTGTTTCCGT CAATTCGTCT 
GCCCCACAAC TGGTGACCAT AAATCACTCG ATTATTATCA ACAAAACGCC GGAGCCACCG 
ATGAACTATA ATAATAATAA TAATAACCAG ACTGCTAAGT CGAGTGGCAC CAGCAGTGTC 
TATGTGAATG TGCCGTCTTT CTCCAGCATG AGCACCAGTT CCTGCTCCAT TAACCAGCCA 
CAATCCTCAC CAGCAGCTGT GATTCCAGTT CAGGCACCTC TTCCAGTTCC AGTTCCTGCC 
CCTGTTTCCG TTCCAGCCGT TCCAGTTCCA ATACCAGCGG TCGTTACCCA TCTGCGTCCT 
TTGCTGCCCA TTGCCGATAA TGCGTATGTG CAAATCTCCC AAGATCGCTG CTCAGATTTC 
ACACCGGACG ACGAGGAGGA ACCCGTGGTG TTGCGTGATG CCGATGTGGT GGCAGACGGA 
ATCGGTATAC CTACCCCTGC GAAACCCAAG AAGGAGAAGT CCAATCTGGC TGTAAGGGTT 
CTGCCGGGAA AGCTCACCCA AAAGGTAAAG GGGCATTCGT ACAAAAAAGT AAGCGCGGCT 
GCCCTTGGAT CCACCAGTTC CCTTAGTTCC GGCAGCAAGC AGAAGCACCA GCGATTGCAG 
TACCAGTCCC ACGATGATGA CGACGACGAA CAGGATCAGG ATGAGAATCG AGGTGCTGGA 
GGCGGCCGCA ATTTCCAATC AAACACCATT CAAAATTCTG CCAAAACCGG CTTCAGCAAC 
ATGAGCTTTG AGGACTTTCC CTCGGATCAG GAAATGGATC GTCTGTCCAG GACCATTCCC 
TTCGAGGTGG TTAGAAACGA AAAGATGTTA CTCGAGGCGG AGAAGAAGTT CGGCTCCCTC 
AAGCGACGGA ACAACTTGTT CTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477166.2
CDS	330..4796
Translation	MKKILSKFERNENSRLENPPNSSSSSSSNEKNSFVGKVFTVGRVTVTVEDVLAEGGFAMVFLARGNGGGSSSATKYALKRMYVNNEHDLNVAKREIQIASNLSGHKNIIGYVDSSITPTGNGVCEVLLLMPYCKHHMLAMMNARLHVGFTEPEVLNIFCDIAEAVSRLHYCQTPIIHRDLKVENILQTDAGNFVLCDFGSATAKTLNPQQHGVTVVQEEIQKYTTLSYRAPEMIDLYGGKSITTKADIWALGCMLYKLCFFSLPFGESTLAIQNGQFSIPDSSKYSKGMHQLIKYMLEPDMEKRPNIWQVCEVAFRLAGKDNPVQNLHKTPIPNFDLLVVPPFESEAKRISAAASKAAKAQNVSVVEAGTSVAPRSRPKGSSSVHGGNPLGLGLPPSPSPRQNITSPQPQPQPVVEQFQANFPAMPPAVPPPPQAVTPAAPAASKLFESSGYPDPFNEPAAAVIPTEFLPAAEEPNKEEVPQDLNVIAGTPTKSMLTPPKPSGGGGHRRNVSDTSAFNKTFANETSQFLAPFNNNLAAMGDGQQTLASAASNPGIYASSTANSAAVSISELMKPGQTAVEKRVEAWNPFEEEQPFSQMTEDHIFEAEFDKIRQRGSQGSITAKSASTTSTLTPTEQIPSAVVAPVTAAPGAVPPPPIAAAVSPYPSQVSEDPFGSAPFSLPPGLREKASSLRKTGAKFIGVSSGGTGGGVATPIGLVAGGANTATSSNSSKWMLSPTLEVSSEEKTSLISKLKTDSECGIPGAVGGAGEGVSGYVKLPVEDRNKYEKLRSKDQPTSDDSDSEASEFFQQDSDEGGGGIFRQMQMVTSNLPETIHKIQQVAYHKVDKTAHLPIVKKLRDKTKKPTTAAHQAAQQLNLMAAAVAAQAEEAAKAAESDGDSIGSASDLRAEDDDLFDENPRQTQAKQRRPLPTEMDGISESVKTCSSSAYHAECESVTTHEDDVRSRVVVKVRMRKKDRALVTATGDPSAPSEELSPTSSDLLHKFGDRPLLLDDELDYGSGESKSSTESPVAPILPTNPDVQESEEDVFALAPFKLPVGVPLKRKTKPKAKPKPSEPEMWTSTPVKSAAVGDPSTNFASFPAQLTPTNNVGNSAQLDEFNEPIFNPFQENAQNTQSGETNQCDLFGLEPFPQVIRTIEQNPQHHQIHQFPSHNNNNNVIKVPASVSVALPASVPVSVSVNSSAPQLVTINHSIIINKTPEPPMNYNNNNNNQTAKSSGTSSVYVNVPSFSSMSTSSCSINQPQSSPAAVIPVQAPLPVPVPAPVSVPAVPVPIPAVVTHLRPLLPIADNAYVQISQDRCSDFTPDDEEEPVVLRDADVVADGIGIPTPAKPKKEKSNLAVRVLPGKLTQKVKGHSYKKVSAAALGSTSSLSSGSKQKHQRLQYQSHDDDDDEQDQDENRGAGGGRNFQSNTIQNSAKTGFSNMSFEDFPSDQEMDRLSRTIPFEVVRNEKMLLEAEKKFGSLKRRNNLFS

Target ORF information:

RefSeq Version	NM_057818.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Numb-associated kinase (Nak), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057818.4

ORF Insert Sequence:

ATGAAGAAAA TCCTGTCTAA GTTCGAGCGC AATGAGAACT CTCGACTGGA GAATCCGCCC 
AACAGCAGCA GCTCGTCGTC CTCGAACGAG AAGAACAGCT TCGTGGGCAA GGTCTTCACA 
GTGGGACGCG TCACGGTCAC CGTGGAGGAT GTCCTGGCAG AAGGTGGTTT CGCCATGGTC 
TTTTTGGCAA GGGGCAACGG CGGAGGCAGT TCCTCGGCGA CCAAGTACGC CCTGAAGCGC 
ATGTACGTCA ACAATGAGCA CGATCTGAAC GTAGCCAAGC GGGAGATACA GATTGCGAGC 
AACCTGAGTG GGCACAAAAA CATCATTGGT TATGTGGACT CCAGCATCAC GCCCACGGGA 
AACGGGGTGT GCGAGGTGCT ACTCCTGATG CCCTATTGCA AGCATCACAT GCTGGCCATG 
ATGAATGCGA GATTGCACGT TGGCTTCACG GAGCCAGAGG TGCTCAACAT CTTCTGTGAT 
ATTGCCGAGG CTGTCTCTCG ATTGCACTAT TGTCAGACTC CGATTATCCA TCGGGATCTT 
AAGGTGGAGA ACATTCTGCA AACGGACGCG GGCAACTTCG TACTCTGCGA CTTCGGATCG 
GCAACGGCCA AGACGTTAAA TCCTCAACAG CACGGAGTCA CCGTGGTGCA GGAAGAGATC 
CAAAAGTACA CCACTCTATC TTATCGGGCT CCCGAGATGA TCGACCTGTA CGGGGGCAAG 
AGCATAACCA CAAAGGCGGA CATCTGGGCA CTGGGTTGCA TGCTCTATAA GCTGTGCTTC 
TTTTCCTTGC CATTTGGGGA GAGCACTTTG GCTATCCAGA ATGGACAGTT TTCCATTCCA 
GACTCCTCAA AGTACTCGAA GGGCATGCAC CAACTGATTA AATATATGTT GGAACCCGAT 
ATGGAGAAGC GACCAAACAT TTGGCAAGTT TGCGAGGTGG CCTTTCGACT GGCGGGCAAG 
GATAATCCCG TTCAGAATCT CCATAAAACT CCCATTCCAA ACTTCGACCT ACTGGTAGTG 
CCTCCGTTTG AATCGGAAGC CAAGAGGATC AGTGCAGCGG CCTCCAAGGC GGCGAAGGCT 
CAGAATGTGT CAGTTGTGGA GGCGGGAACC AGTGTAGCTC CTCGGTCTCG TCCTAAGGGT 
TCCTCATCGG TACATGGAGG AAATCCATTG GGTCTCGGTT TGCCTCCCAG TCCCTCGCCA 
AGACAGAATA TCACTTCTCC ACAACCCCAA CCTCAACCTG TGGTCGAGCA GTTCCAGGCC 
AACTTCCCAG CTATGCCACC TGCTGTTCCT CCACCTCCTC AAGCTGTTAC TCCGGCAGCT 
CCTGCTGCCA GTAAACTCTT CGAGTCAAGT GGCTATCCAG ATCCATTCAA TGAGCCAGCT 
GCAGCAGTAA TTCCTACTGA ATTTCTACCA GCAGCAGAAG AACCTAACAA GGAGGAAGTG 
CCTCAGGACT TAAACGTCAT TGCAGGCACG CCCACCAAGA GCATGTTGAC ACCGCCCAAG 
CCAAGCGGAG GAGGTGGTCA TAGGCGTAAC GTAAGCGACA CCTCGGCCTT CAATAAAACT 
TTTGCCAATG AGACAAGTCA GTTTCTGGCT CCGTTTAACA ATAACTTGGC TGCCATGGGC 
GACGGTCAGC AGACTTTGGC TAGTGCTGCC TCGAATCCCG GGATATATGC ATCTTCAACG 
GCAAACTCAG CCGCGGTATC CATTAGCGAA TTAATGAAAC CAGGACAAAC GGCGGTGGAA 
AAGCGAGTTG AAGCCTGGAA TCCGTTCGAA GAGGAGCAGC CTTTCAGCCA GATGACTGAG 
GATCACATCT TTGAGGCGGA GTTCGACAAG ATACGACAGA GAGGCAGTCA AGGAAGCATC 
ACAGCAAAAT CGGCATCCAC AACTTCCACG CTAACACCAA CGGAGCAGAT TCCCTCTGCG 
GTTGTGGCTC CTGTTACAGC TGCTCCTGGT GCAGTGCCTC CTCCGCCGAT TGCTGCCGCT 
GTGTCTCCTT ACCCGTCGCA GGTCTCCGAG GATCCATTCG GATCGGCGCC ATTCAGTTTG 
CCACCCGGAC TGCGCGAGAA GGCGAGCTCG CTGCGCAAAA CCGGAGCGAA ATTCATCGGC 
GTTTCATCTG GTGGAACAGG AGGCGGAGTG GCCACGCCTA TCGGCTTGGT AGCGGGAGGA 
GCAAACACCG CCACCTCTTC CAACTCATCC AAGTGGATGC TCTCCCCCAC CCTGGAGGTT 
TCCAGCGAAG AGAAGACCTC GCTTATCAGT AAACTCAAAA CAGACTCAGA ATGTGGAATT 
CCAGGCGCTG TTGGAGGAGC TGGAGAAGGT GTCTCTGGTT ATGTTAAGCT ACCTGTAGAA 
GATCGCAACA AGTACGAGAA GCTGCGCAGC AAAGATCAGC CGACTTCCGA TGACTCCGAT 
TCCGAGGCTT CGGAGTTCTT TCAACAAGAC AGCGATGAAG GAGGTGGGGG CATCTTTAGG 
CAAATGCAGA TGGTGACAAG CAATTTGCCG GAAACCATCC ATAAAATCCA ACAGGTTGCC 
TATCACAAGG TGGACAAGAC AGCTCATCTG CCAATTGTGA AGAAACTTCG CGATAAGACC 
AAGAAACCCA CAACAGCTGC CCATCAAGCG GCCCAACAAC TAAATCTCAT GGCTGCCGCG 
GTAGCTGCAC AAGCTGAAGA AGCTGCCAAG GCGGCGGAAT CCGACGGTGA TTCGATCGGA 
TCTGCCAGTG ACCTGCGGGC AGAGGATGAT GATCTGTTTG ACGAGAATCC TCGCCAGACA 
CAAGCCAAGC AACGTCGTCC TCTGCCAACA GAAATGGATG GAATAAGCGA GTCAGTGAAG 
ACCTGTAGCA GCTCTGCCTA CCATGCAGAA TGTGAAAGTG TGACCACCCA TGAAGACGAT 
GTGCGTAGTC GGGTGGTGGT GAAGGTGCGG ATGAGGAAGA AGGACCGTGC CCTTGTGACT 
GCCACTGGCG ACCCAAGTGC TCCGTCTGAA GAACTAAGTC CCACTTCGAG CGATCTCTTG 
CACAAGTTCG GCGATCGTCC GTTGCTGCTA GATGATGAAT TGGATTATGG TTCTGGAGAA 
AGCAAGAGTA GCACTGAATC TCCTGTGGCT CCAATTCTGC CAACAAATCC CGATGTGCAG 
GAATCAGAGG AGGATGTGTT CGCCCTGGCT CCATTTAAAT TACCAGTCGG AGTTCCTTTG 
AAACGAAAGA CTAAACCCAA AGCTAAGCCC AAGCCTTCGG AACCCGAGAT GTGGACTTCA 
ACGCCAGTTA AAAGCGCCGC TGTCGGAGAT CCTTCTACCA ACTTTGCCAG TTTTCCAGCG 
CAGCTCACGC CAACCAATAA TGTGGGGAAC TCTGCGCAGC TGGATGAGTT CAATGAGCCA 
ATTTTCAATC CCTTCCAGGA AAACGCACAG AACACGCAAT CAGGGGAAAC TAATCAATGC 
GACCTGTTCG GCTTGGAACC ATTTCCACAG GTAATTCGTA CAATCGAACA GAACCCACAG 
CACCACCAGA TCCACCAATT TCCCAGCCAC AACAACAATA ACAATGTGAT CAAAGTGCCC 
GCCTCCGTAT CTGTTGCTCT TCCTGCCTCC GTTCCTGTAT CTGTTTCCGT CAATTCGTCT 
GCCCCACAAC TGGTGACCAT AAATCACTCG ATTATTATCA ACAAAACGCC GGAGCCACCG 
ATGAACTATA ATAATAATAA TAATAACCAG ACTGCTAAGT CGAGTGGCAC CAGCAGTGTC 
TATGTGAATG TGCCGTCTTT CTCCAGCATG AGCACCAGTT CCTGCTCCAT TAACCAGCCA 
CAATCCTCAC CAGCAGCTGT GATTCCAGTT CAGGCACCTC TTCCAGTTCC AGTTCCTGCC 
CCTGTTTCCG TTCCAGCCGT TCCAGTTCCA ATACCAGCGG TCGTTACCCA TCTGCGTCCT 
TTGCTGCCCA TTGCCGATAA TGCGTATGTG CAAATCTCCC AAGATCGCTG CTCAGATTTC 
ACACCGGACG ACGAGGAGGA ACCCGTGGTG TTGCGTGATG CCGATGTGGT GGCAGACGGA 
ATCGGTATAC CTACCCCTGC GAAACCCAAG AAGGAGAAGT CCAATCTGGC TGTAAGGGTT 
CTGCCGGGAA AGCTCACCCA AAAGGTAAAG GGGCATTCGT ACAAAAAAGT AAGCGCGGCT 
GCCCTTGGAT CCACCAGTTC CCTTAGTTCC GGCAGCAAGC AGAAGCACCA GCGATTGCAG 
TACCAGTCCC ACGATGATGA CGACGACGAA CAGGATCAGG ATGAGAATCG AGGTGCTGGA 
GGCGGCCGCA ATTTCCAATC AAACACCATT CAAAATTCTG CCAAAACCGG CTTCAGCAAC 
ATGAGCTTTG AGGACTTTCC CTCGGATCAG GAAATGGATC GTCTGTCCAG GACCATTCCC 
TTCGAGGTGG TTAGAAACGA AAAGATGTTA CTCGAGGCGG AGAAGAAGTT CGGCTCCCTC 
AAGCGACGGA ACAACTTGTT CTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nak ( NM_057817.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477165.1 Drosophila melanogaster Numb-associated kinase (Nak), transcript variant A, mRNA.

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CloneID	OFa16022
Clone ID Related Accession (Same CDS sequence)	NM_057818.4 , NM_057817.4
Accession Version	NM_057817.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4467bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Numb-associated kinase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_057817.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGAAAA TCCTGTCTAA GTTCGAGCGC AATGAGAACT CTCGACTGGA GAATCCGCCC 
AACAGCAGCA GCTCGTCGTC CTCGAACGAG AAGAACAGCT TCGTGGGCAA GGTCTTCACA 
GTGGGACGCG TCACGGTCAC CGTGGAGGAT GTCCTGGCAG AAGGTGGTTT CGCCATGGTC 
TTTTTGGCAA GGGGCAACGG CGGAGGCAGT TCCTCGGCGA CCAAGTACGC CCTGAAGCGC 
ATGTACGTCA ACAATGAGCA CGATCTGAAC GTAGCCAAGC GGGAGATACA GATTGCGAGC 
AACCTGAGTG GGCACAAAAA CATCATTGGT TATGTGGACT CCAGCATCAC GCCCACGGGA 
AACGGGGTGT GCGAGGTGCT ACTCCTGATG CCCTATTGCA AGCATCACAT GCTGGCCATG 
ATGAATGCGA GATTGCACGT TGGCTTCACG GAGCCAGAGG TGCTCAACAT CTTCTGTGAT 
ATTGCCGAGG CTGTCTCTCG ATTGCACTAT TGTCAGACTC CGATTATCCA TCGGGATCTT 
AAGGTGGAGA ACATTCTGCA AACGGACGCG GGCAACTTCG TACTCTGCGA CTTCGGATCG 
GCAACGGCCA AGACGTTAAA TCCTCAACAG CACGGAGTCA CCGTGGTGCA GGAAGAGATC 
CAAAAGTACA CCACTCTATC TTATCGGGCT CCCGAGATGA TCGACCTGTA CGGGGGCAAG 
AGCATAACCA CAAAGGCGGA CATCTGGGCA CTGGGTTGCA TGCTCTATAA GCTGTGCTTC 
TTTTCCTTGC CATTTGGGGA GAGCACTTTG GCTATCCAGA ATGGACAGTT TTCCATTCCA 
GACTCCTCAA AGTACTCGAA GGGCATGCAC CAACTGATTA AATATATGTT GGAACCCGAT 
ATGGAGAAGC GACCAAACAT TTGGCAAGTT TGCGAGGTGG CCTTTCGACT GGCGGGCAAG 
GATAATCCCG TTCAGAATCT CCATAAAACT CCCATTCCAA ACTTCGACCT ACTGGTAGTG 
CCTCCGTTTG AATCGGAAGC CAAGAGGATC AGTGCAGCGG CCTCCAAGGC GGCGAAGGCT 
CAGAATGTGT CAGTTGTGGA GGCGGGAACC AGTGTAGCTC CTCGGTCTCG TCCTAAGGGT 
TCCTCATCGG TACATGGAGG AAATCCATTG GGTCTCGGTT TGCCTCCCAG TCCCTCGCCA 
AGACAGAATA TCACTTCTCC ACAACCCCAA CCTCAACCTG TGGTCGAGCA GTTCCAGGCC 
AACTTCCCAG CTATGCCACC TGCTGTTCCT CCACCTCCTC AAGCTGTTAC TCCGGCAGCT 
CCTGCTGCCA GTAAACTCTT CGAGTCAAGT GGCTATCCAG ATCCATTCAA TGAGCCAGCT 
GCAGCAGTAA TTCCTACTGA ATTTCTACCA GCAGCAGAAG AACCTAACAA GGAGGAAGTG 
CCTCAGGACT TAAACGTCAT TGCAGGCACG CCCACCAAGA GCATGTTGAC ACCGCCCAAG 
CCAAGCGGAG GAGGTGGTCA TAGGCGTAAC GTAAGCGACA CCTCGGCCTT CAATAAAACT 
TTTGCCAATG AGACAAGTCA GTTTCTGGCT CCGTTTAACA ATAACTTGGC TGCCATGGGC 
GACGGTCAGC AGACTTTGGC TAGTGCTGCC TCGAATCCCG GGATATATGC ATCTTCAACG 
GCAAACTCAG CCGCGGTATC CATTAGCGAA TTAATGAAAC CAGGACAAAC GGCGGTGGAA 
AAGCGAGTTG AAGCCTGGAA TCCGTTCGAA GAGGAGCAGC CTTTCAGCCA GATGACTGAG 
GATCACATCT TTGAGGCGGA GTTCGACAAG ATACGACAGA GAGGCAGTCA AGGAAGCATC 
ACAGCAAAAT CGGCATCCAC AACTTCCACG CTAACACCAA CGGAGCAGAT TCCCTCTGCG 
GTTGTGGCTC CTGTTACAGC TGCTCCTGGT GCAGTGCCTC CTCCGCCGAT TGCTGCCGCT 
GTGTCTCCTT ACCCGTCGCA GGTCTCCGAG GATCCATTCG GATCGGCGCC ATTCAGTTTG 
CCACCCGGAC TGCGCGAGAA GGCGAGCTCG CTGCGCAAAA CCGGAGCGAA ATTCATCGGC 
GTTTCATCTG GTGGAACAGG AGGCGGAGTG GCCACGCCTA TCGGCTTGGT AGCGGGAGGA 
GCAAACACCG CCACCTCTTC CAACTCATCC AAGTGGATGC TCTCCCCCAC CCTGGAGGTT 
TCCAGCGAAG AGAAGACCTC GCTTATCAGT AAACTCAAAA CAGACTCAGA ATGTGGAATT 
CCAGGCGCTG TTGGAGGAGC TGGAGAAGGT GTCTCTGGTT ATGTTAAGCT ACCTGTAGAA 
GATCGCAACA AGTACGAGAA GCTGCGCAGC AAAGATCAGC CGACTTCCGA TGACTCCGAT 
TCCGAGGCTT CGGAGTTCTT TCAACAAGAC AGCGATGAAG GAGGTGGGGG CATCTTTAGG 
CAAATGCAGA TGGTGACAAG CAATTTGCCG GAAACCATCC ATAAAATCCA ACAGGTTGCC 
TATCACAAGG TGGACAAGAC AGCTCATCTG CCAATTGTGA AGAAACTTCG CGATAAGACC 
AAGAAACCCA CAACAGCTGC CCATCAAGCG GCCCAACAAC TAAATCTCAT GGCTGCCGCG 
GTAGCTGCAC AAGCTGAAGA AGCTGCCAAG GCGGCGGAAT CCGACGGTGA TTCGATCGGA 
TCTGCCAGTG ACCTGCGGGC AGAGGATGAT GATCTGTTTG ACGAGAATCC TCGCCAGACA 
CAAGCCAAGC AACGTCGTCC TCTGCCAACA GAAATGGATG GAATAAGCGA GTCAGTGAAG 
ACCTGTAGCA GCTCTGCCTA CCATGCAGAA TGTGAAAGTG TGACCACCCA TGAAGACGAT 
GTGCGTAGTC GGGTGGTGGT GAAGGTGCGG ATGAGGAAGA AGGACCGTGC CCTTGTGACT 
GCCACTGGCG ACCCAAGTGC TCCGTCTGAA GAACTAAGTC CCACTTCGAG CGATCTCTTG 
CACAAGTTCG GCGATCGTCC GTTGCTGCTA GATGATGAAT TGGATTATGG TTCTGGAGAA 
AGCAAGAGTA GCACTGAATC TCCTGTGGCT CCAATTCTGC CAACAAATCC CGATGTGCAG 
GAATCAGAGG AGGATGTGTT CGCCCTGGCT CCATTTAAAT TACCAGTCGG AGTTCCTTTG 
AAACGAAAGA CTAAACCCAA AGCTAAGCCC AAGCCTTCGG AACCCGAGAT GTGGACTTCA 
ACGCCAGTTA AAAGCGCCGC TGTCGGAGAT CCTTCTACCA ACTTTGCCAG TTTTCCAGCG 
CAGCTCACGC CAACCAATAA TGTGGGGAAC TCTGCGCAGC TGGATGAGTT CAATGAGCCA 
ATTTTCAATC CCTTCCAGGA AAACGCACAG AACACGCAAT CAGGGGAAAC TAATCAATGC 
GACCTGTTCG GCTTGGAACC ATTTCCACAG GTAATTCGTA CAATCGAACA GAACCCACAG 
CACCACCAGA TCCACCAATT TCCCAGCCAC AACAACAATA ACAATGTGAT CAAAGTGCCC 
GCCTCCGTAT CTGTTGCTCT TCCTGCCTCC GTTCCTGTAT CTGTTTCCGT CAATTCGTCT 
GCCCCACAAC TGGTGACCAT AAATCACTCG ATTATTATCA ACAAAACGCC GGAGCCACCG 
ATGAACTATA ATAATAATAA TAATAACCAG ACTGCTAAGT CGAGTGGCAC CAGCAGTGTC 
TATGTGAATG TGCCGTCTTT CTCCAGCATG AGCACCAGTT CCTGCTCCAT TAACCAGCCA 
CAATCCTCAC CAGCAGCTGT GATTCCAGTT CAGGCACCTC TTCCAGTTCC AGTTCCTGCC 
CCTGTTTCCG TTCCAGCCGT TCCAGTTCCA ATACCAGCGG TCGTTACCCA TCTGCGTCCT 
TTGCTGCCCA TTGCCGATAA TGCGTATGTG CAAATCTCCC AAGATCGCTG CTCAGATTTC 
ACACCGGACG ACGAGGAGGA ACCCGTGGTG TTGCGTGATG CCGATGTGGT GGCAGACGGA 
ATCGGTATAC CTACCCCTGC GAAACCCAAG AAGGAGAAGT CCAATCTGGC TGTAAGGGTT 
CTGCCGGGAA AGCTCACCCA AAAGGTAAAG GGGCATTCGT ACAAAAAAGT AAGCGCGGCT 
GCCCTTGGAT CCACCAGTTC CCTTAGTTCC GGCAGCAAGC AGAAGCACCA GCGATTGCAG 
TACCAGTCCC ACGATGATGA CGACGACGAA CAGGATCAGG ATGAGAATCG AGGTGCTGGA 
GGCGGCCGCA ATTTCCAATC AAACACCATT CAAAATTCTG CCAAAACCGG CTTCAGCAAC 
ATGAGCTTTG AGGACTTTCC CTCGGATCAG GAAATGGATC GTCTGTCCAG GACCATTCCC 
TTCGAGGTGG TTAGAAACGA AAAGATGTTA CTCGAGGCGG AGAAGAAGTT CGGCTCCCTC 
AAGCGACGGA ACAACTTGTT CTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477165.1
CDS	330..4796
Translation	MKKILSKFERNENSRLENPPNSSSSSSSNEKNSFVGKVFTVGRVTVTVEDVLAEGGFAMVFLARGNGGGSSSATKYALKRMYVNNEHDLNVAKREIQIASNLSGHKNIIGYVDSSITPTGNGVCEVLLLMPYCKHHMLAMMNARLHVGFTEPEVLNIFCDIAEAVSRLHYCQTPIIHRDLKVENILQTDAGNFVLCDFGSATAKTLNPQQHGVTVVQEEIQKYTTLSYRAPEMIDLYGGKSITTKADIWALGCMLYKLCFFSLPFGESTLAIQNGQFSIPDSSKYSKGMHQLIKYMLEPDMEKRPNIWQVCEVAFRLAGKDNPVQNLHKTPIPNFDLLVVPPFESEAKRISAAASKAAKAQNVSVVEAGTSVAPRSRPKGSSSVHGGNPLGLGLPPSPSPRQNITSPQPQPQPVVEQFQANFPAMPPAVPPPPQAVTPAAPAASKLFESSGYPDPFNEPAAAVIPTEFLPAAEEPNKEEVPQDLNVIAGTPTKSMLTPPKPSGGGGHRRNVSDTSAFNKTFANETSQFLAPFNNNLAAMGDGQQTLASAASNPGIYASSTANSAAVSISELMKPGQTAVEKRVEAWNPFEEEQPFSQMTEDHIFEAEFDKIRQRGSQGSITAKSASTTSTLTPTEQIPSAVVAPVTAAPGAVPPPPIAAAVSPYPSQVSEDPFGSAPFSLPPGLREKASSLRKTGAKFIGVSSGGTGGGVATPIGLVAGGANTATSSNSSKWMLSPTLEVSSEEKTSLISKLKTDSECGIPGAVGGAGEGVSGYVKLPVEDRNKYEKLRSKDQPTSDDSDSEASEFFQQDSDEGGGGIFRQMQMVTSNLPETIHKIQQVAYHKVDKTAHLPIVKKLRDKTKKPTTAAHQAAQQLNLMAAAVAAQAEEAAKAAESDGDSIGSASDLRAEDDDLFDENPRQTQAKQRRPLPTEMDGISESVKTCSSSAYHAECESVTTHEDDVRSRVVVKVRMRKKDRALVTATGDPSAPSEELSPTSSDLLHKFGDRPLLLDDELDYGSGESKSSTESPVAPILPTNPDVQESEEDVFALAPFKLPVGVPLKRKTKPKAKPKPSEPEMWTSTPVKSAAVGDPSTNFASFPAQLTPTNNVGNSAQLDEFNEPIFNPFQENAQNTQSGETNQCDLFGLEPFPQVIRTIEQNPQHHQIHQFPSHNNNNNVIKVPASVSVALPASVPVSVSVNSSAPQLVTINHSIIINKTPEPPMNYNNNNNNQTAKSSGTSSVYVNVPSFSSMSTSSCSINQPQSSPAAVIPVQAPLPVPVPAPVSVPAVPVPIPAVVTHLRPLLPIADNAYVQISQDRCSDFTPDDEEEPVVLRDADVVADGIGIPTPAKPKKEKSNLAVRVLPGKLTQKVKGHSYKKVSAAALGSTSSLSSGSKQKHQRLQYQSHDDDDDEQDQDENRGAGGGRNFQSNTIQNSAKTGFSNMSFEDFPSDQEMDRLSRTIPFEVVRNEKMLLEAEKKFGSLKRRNNLFS

Target ORF information:

RefSeq Version	NM_057817.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Numb-associated kinase (Nak), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057817.4

ORF Insert Sequence:

ATGAAGAAAA TCCTGTCTAA GTTCGAGCGC AATGAGAACT CTCGACTGGA GAATCCGCCC 
AACAGCAGCA GCTCGTCGTC CTCGAACGAG AAGAACAGCT TCGTGGGCAA GGTCTTCACA 
GTGGGACGCG TCACGGTCAC CGTGGAGGAT GTCCTGGCAG AAGGTGGTTT CGCCATGGTC 
TTTTTGGCAA GGGGCAACGG CGGAGGCAGT TCCTCGGCGA CCAAGTACGC CCTGAAGCGC 
ATGTACGTCA ACAATGAGCA CGATCTGAAC GTAGCCAAGC GGGAGATACA GATTGCGAGC 
AACCTGAGTG GGCACAAAAA CATCATTGGT TATGTGGACT CCAGCATCAC GCCCACGGGA 
AACGGGGTGT GCGAGGTGCT ACTCCTGATG CCCTATTGCA AGCATCACAT GCTGGCCATG 
ATGAATGCGA GATTGCACGT TGGCTTCACG GAGCCAGAGG TGCTCAACAT CTTCTGTGAT 
ATTGCCGAGG CTGTCTCTCG ATTGCACTAT TGTCAGACTC CGATTATCCA TCGGGATCTT 
AAGGTGGAGA ACATTCTGCA AACGGACGCG GGCAACTTCG TACTCTGCGA CTTCGGATCG 
GCAACGGCCA AGACGTTAAA TCCTCAACAG CACGGAGTCA CCGTGGTGCA GGAAGAGATC 
CAAAAGTACA CCACTCTATC TTATCGGGCT CCCGAGATGA TCGACCTGTA CGGGGGCAAG 
AGCATAACCA CAAAGGCGGA CATCTGGGCA CTGGGTTGCA TGCTCTATAA GCTGTGCTTC 
TTTTCCTTGC CATTTGGGGA GAGCACTTTG GCTATCCAGA ATGGACAGTT TTCCATTCCA 
GACTCCTCAA AGTACTCGAA GGGCATGCAC CAACTGATTA AATATATGTT GGAACCCGAT 
ATGGAGAAGC GACCAAACAT TTGGCAAGTT TGCGAGGTGG CCTTTCGACT GGCGGGCAAG 
GATAATCCCG TTCAGAATCT CCATAAAACT CCCATTCCAA ACTTCGACCT ACTGGTAGTG 
CCTCCGTTTG AATCGGAAGC CAAGAGGATC AGTGCAGCGG CCTCCAAGGC GGCGAAGGCT 
CAGAATGTGT CAGTTGTGGA GGCGGGAACC AGTGTAGCTC CTCGGTCTCG TCCTAAGGGT 
TCCTCATCGG TACATGGAGG AAATCCATTG GGTCTCGGTT TGCCTCCCAG TCCCTCGCCA 
AGACAGAATA TCACTTCTCC ACAACCCCAA CCTCAACCTG TGGTCGAGCA GTTCCAGGCC 
AACTTCCCAG CTATGCCACC TGCTGTTCCT CCACCTCCTC AAGCTGTTAC TCCGGCAGCT 
CCTGCTGCCA GTAAACTCTT CGAGTCAAGT GGCTATCCAG ATCCATTCAA TGAGCCAGCT 
GCAGCAGTAA TTCCTACTGA ATTTCTACCA GCAGCAGAAG AACCTAACAA GGAGGAAGTG 
CCTCAGGACT TAAACGTCAT TGCAGGCACG CCCACCAAGA GCATGTTGAC ACCGCCCAAG 
CCAAGCGGAG GAGGTGGTCA TAGGCGTAAC GTAAGCGACA CCTCGGCCTT CAATAAAACT 
TTTGCCAATG AGACAAGTCA GTTTCTGGCT CCGTTTAACA ATAACTTGGC TGCCATGGGC 
GACGGTCAGC AGACTTTGGC TAGTGCTGCC TCGAATCCCG GGATATATGC ATCTTCAACG 
GCAAACTCAG CCGCGGTATC CATTAGCGAA TTAATGAAAC CAGGACAAAC GGCGGTGGAA 
AAGCGAGTTG AAGCCTGGAA TCCGTTCGAA GAGGAGCAGC CTTTCAGCCA GATGACTGAG 
GATCACATCT TTGAGGCGGA GTTCGACAAG ATACGACAGA GAGGCAGTCA AGGAAGCATC 
ACAGCAAAAT CGGCATCCAC AACTTCCACG CTAACACCAA CGGAGCAGAT TCCCTCTGCG 
GTTGTGGCTC CTGTTACAGC TGCTCCTGGT GCAGTGCCTC CTCCGCCGAT TGCTGCCGCT 
GTGTCTCCTT ACCCGTCGCA GGTCTCCGAG GATCCATTCG GATCGGCGCC ATTCAGTTTG 
CCACCCGGAC TGCGCGAGAA GGCGAGCTCG CTGCGCAAAA CCGGAGCGAA ATTCATCGGC 
GTTTCATCTG GTGGAACAGG AGGCGGAGTG GCCACGCCTA TCGGCTTGGT AGCGGGAGGA 
GCAAACACCG CCACCTCTTC CAACTCATCC AAGTGGATGC TCTCCCCCAC CCTGGAGGTT 
TCCAGCGAAG AGAAGACCTC GCTTATCAGT AAACTCAAAA CAGACTCAGA ATGTGGAATT 
CCAGGCGCTG TTGGAGGAGC TGGAGAAGGT GTCTCTGGTT ATGTTAAGCT ACCTGTAGAA 
GATCGCAACA AGTACGAGAA GCTGCGCAGC AAAGATCAGC CGACTTCCGA TGACTCCGAT 
TCCGAGGCTT CGGAGTTCTT TCAACAAGAC AGCGATGAAG GAGGTGGGGG CATCTTTAGG 
CAAATGCAGA TGGTGACAAG CAATTTGCCG GAAACCATCC ATAAAATCCA ACAGGTTGCC 
TATCACAAGG TGGACAAGAC AGCTCATCTG CCAATTGTGA AGAAACTTCG CGATAAGACC 
AAGAAACCCA CAACAGCTGC CCATCAAGCG GCCCAACAAC TAAATCTCAT GGCTGCCGCG 
GTAGCTGCAC AAGCTGAAGA AGCTGCCAAG GCGGCGGAAT CCGACGGTGA TTCGATCGGA 
TCTGCCAGTG ACCTGCGGGC AGAGGATGAT GATCTGTTTG ACGAGAATCC TCGCCAGACA 
CAAGCCAAGC AACGTCGTCC TCTGCCAACA GAAATGGATG GAATAAGCGA GTCAGTGAAG 
ACCTGTAGCA GCTCTGCCTA CCATGCAGAA TGTGAAAGTG TGACCACCCA TGAAGACGAT 
GTGCGTAGTC GGGTGGTGGT GAAGGTGCGG ATGAGGAAGA AGGACCGTGC CCTTGTGACT 
GCCACTGGCG ACCCAAGTGC TCCGTCTGAA GAACTAAGTC CCACTTCGAG CGATCTCTTG 
CACAAGTTCG GCGATCGTCC GTTGCTGCTA GATGATGAAT TGGATTATGG TTCTGGAGAA 
AGCAAGAGTA GCACTGAATC TCCTGTGGCT CCAATTCTGC CAACAAATCC CGATGTGCAG 
GAATCAGAGG AGGATGTGTT CGCCCTGGCT CCATTTAAAT TACCAGTCGG AGTTCCTTTG 
AAACGAAAGA CTAAACCCAA AGCTAAGCCC AAGCCTTCGG AACCCGAGAT GTGGACTTCA 
ACGCCAGTTA AAAGCGCCGC TGTCGGAGAT CCTTCTACCA ACTTTGCCAG TTTTCCAGCG 
CAGCTCACGC CAACCAATAA TGTGGGGAAC TCTGCGCAGC TGGATGAGTT CAATGAGCCA 
ATTTTCAATC CCTTCCAGGA AAACGCACAG AACACGCAAT CAGGGGAAAC TAATCAATGC 
GACCTGTTCG GCTTGGAACC ATTTCCACAG GTAATTCGTA CAATCGAACA GAACCCACAG 
CACCACCAGA TCCACCAATT TCCCAGCCAC AACAACAATA ACAATGTGAT CAAAGTGCCC 
GCCTCCGTAT CTGTTGCTCT TCCTGCCTCC GTTCCTGTAT CTGTTTCCGT CAATTCGTCT 
GCCCCACAAC TGGTGACCAT AAATCACTCG ATTATTATCA ACAAAACGCC GGAGCCACCG 
ATGAACTATA ATAATAATAA TAATAACCAG ACTGCTAAGT CGAGTGGCAC CAGCAGTGTC 
TATGTGAATG TGCCGTCTTT CTCCAGCATG AGCACCAGTT CCTGCTCCAT TAACCAGCCA 
CAATCCTCAC CAGCAGCTGT GATTCCAGTT CAGGCACCTC TTCCAGTTCC AGTTCCTGCC 
CCTGTTTCCG TTCCAGCCGT TCCAGTTCCA ATACCAGCGG TCGTTACCCA TCTGCGTCCT 
TTGCTGCCCA TTGCCGATAA TGCGTATGTG CAAATCTCCC AAGATCGCTG CTCAGATTTC 
ACACCGGACG ACGAGGAGGA ACCCGTGGTG TTGCGTGATG CCGATGTGGT GGCAGACGGA 
ATCGGTATAC CTACCCCTGC GAAACCCAAG AAGGAGAAGT CCAATCTGGC TGTAAGGGTT 
CTGCCGGGAA AGCTCACCCA AAAGGTAAAG GGGCATTCGT ACAAAAAAGT AAGCGCGGCT 
GCCCTTGGAT CCACCAGTTC CCTTAGTTCC GGCAGCAAGC AGAAGCACCA GCGATTGCAG 
TACCAGTCCC ACGATGATGA CGACGACGAA CAGGATCAGG ATGAGAATCG AGGTGCTGGA 
GGCGGCCGCA ATTTCCAATC AAACACCATT CAAAATTCTG CCAAAACCGG CTTCAGCAAC 
ATGAGCTTTG AGGACTTTCC CTCGGATCAG GAAATGGATC GTCTGTCCAG GACCATTCCC 
TTCGAGGTGG TTAGAAACGA AAAGATGTTA CTCGAGGCGG AGAAGAAGTT CGGCTCCCTC 
AAGCGACGGA ACAACTTGTT CTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nak cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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