ham cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ham
Entrez Gene ID	35135
Full Name	hamlet
Synonyms	CG10568,CG15906,CG15907,CG31753,Dmel\CG31753,HAM_DROME,Ham
General protein information	Preferred Names hamlet Names CG31753 gene product from transcript CG31753-RD CG31753-PB CG31753-PC CG31753-PD CG31753-PE CG31753-PF ham-PB ham-PC ham-PD ham-PE ham-PF
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 37A2-37A4

mRNA and Protein(s)

mRNA	Protein	Name
NM_165262.3	NP_724130.3	hamlet, isoform C
NM_001273616.1	NP_001260545.1	hamlet, isoform F
NM_001273615.1	NP_001260544.1	hamlet, isoform E
NM_001259152.1	NP_001246081.1	hamlet, isoform B
NM_001273614.1	NP_001260543.1	hamlet, isoform D

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	ham	NP_001260543.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008232	XP_317239.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

analysis of physical and functional interactions between Drosophila homologue of Swc6/p18Hamlet subunit of the SWR1/SRCAP chromatin-remodeling complex with the DNA repair/transcription factor TFIIH
Title: Physical and functional interactions between Drosophila homologue of Swc6/p18Hamlet subunit of the SWR1/SRCAP chromatin-remodeling complex with the DNA repair/transcription factor TFIIH.

This study demonistrated that Ham modifies cellular responses to these iteratively used Notch signals in a context-dependent manner, and controls odorant receptor gene choice and ORN axon targeting specificity.
Title: Chromatin modification of Notch targets in olfactory receptor neuron diversification.

The following ham gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ham cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa14923	NM_001259152.1 Latest version!	Drosophila melanogaster hamlet (ham), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa14923	NM_001273614.1 Latest version!	Drosophila melanogaster hamlet (ham), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa14923	NM_165262.3 Latest version!	Drosophila melanogaster hamlet (ham), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa16007	NM_001273616.1 Latest version!	Drosophila melanogaster hamlet (ham), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa16008	NM_001273615.1 Latest version!	Drosophila melanogaster hamlet (ham), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ham ( NM_165262.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724130.3 Drosophila melanogaster hamlet (ham), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa14923
Clone ID Related Accession (Same CDS sequence)	NM_165262.3 , NM_001259152.1 , NM_001273614.1
Accession Version	NM_165262.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2973bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	hamlet, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On May 8, 2012 this sequence version replaced NM_165262.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGCCT TCTTCAAGGA CCGCGCCCAG GCGGAGCACA TCCTGCAGGA GTGGGTGCGT 
AGGCGCGAAC CAGTCTGTGA GCTGGACATC CGTGATAGTG GTGGCGTCTA CGCCAAGACG 
CCGCTCCAGA GGGGCACTCG CTACGGACCC TTTCCCATGA AGCTCAGCCA CCAACCCAAC 
GATCCTCAGC TGGCCTGGAA GGCCCATAGT AGACACTATA ATGGATGGCT AGAGCCCACA 
GAAGATGTGT CCACGTGGTT GAAAAAAATC CGAAGCGTTC AGGACGATTG TATAGGCGAG 
GCTAATTTAC AGAGTTACAT TAATGCTGGT TATCTGTGGT ACGAGACGAA TCGGTACGTG 
AATGCTGGCA GTGAAATGGT CGTCGACGGG CGACCCAAGA GTCCTGTTCA ACTGAACGAG 
GACTTCATGA ACGGCGGCAA GGTCCTGGCA GCAGCCGCAG CAGCAGCCGC CGCAGTGGTG 
GCCGCCTCCT CCTCGGGAGC AAAAAGCGGC GGTGGGGGCA GCTCCGCGCC CAGCGATGAT 
CGCAGCGATC GTGATAATGG TTCTCTGTAC AGTGGTGACG AATTCTCCAA AGATAAGAAA 
AACTCCTCGT TAATCAGAGA GGGCGACATC GATTTCACAG ACGACGAAAA CGGTTTCGAT 
ATACGCTGTG AGGTCTGTGA TAAAGTCTAT CCGGATTTAG ATCTCTTGGA CGATCATCTG 
ATAGGCGCTC ACCATTTCAA GCAGGACGAA TTTCCGTGCA AGCAATGCGC TCTGCGATTT 
TGCCATCGTC CTTTGCTAAT TAAACACGAA GCTATCTCCC ACAATAACAT ACGCAAATAT 
TCCTGCGAAA ATTGCAGCAA GGTATTTTGT GACCCCTCGA ATCTACAGCG GCATATTCGC 
GCCTACCACG TCGGAGCTCG CTGTCATCCG TGTCCGGAGT GCGGGAAAAC GTTCGGAACC 
TCGTCGGGCC TGAAGCAGCA CCAGCACATC CACAGCTCCG TGAAGCCCTT CGCCTGCGAG 
GTGTGCTCCA AGGCGTATAC GCAATTCTCG AATCTGTGCC GTCACAAGCG GATGCACGCC 
ACCTGTCGGA TGCAGATCAA GTGCGACAAG TGCAACCAGA GCTTCAGCAC GTTGACCTCC 
CTGACCAAGC ACAAAAAGTT CTGTGATTCA ACGGGTCCGG GTCCGTATCG AAACCAGCAC 
GTGAATCGCC ACCATCAGCA TCCGCACCAG CACCCACTTC CGCATCAGCC CCACCTGGCC 
GCCACTGCCA CATCCACCTG TCCAGCGCCA CCAAGAGAAT CTTCGGAGTC CTCGTCCTCA 
GCAGCAGCTG CCGCCGTTGC CGCCATGTCG ACGCCACCGA ATCCTTTCCT AATGTTCCGC 
ACTGCGCCGT CTTTCTTTCC CGGATTTCCG CCTTACGGAT TTCCCCCCTT TCTACCACAA 
AATCCGCTGC ATCCTACGAA CATTCCGATG TTTTTCTCGA AAAATCCCAT GGATTTGGGT 
TGCGGTGGTC CGGAGATCAC TTCTCCCGTT TCCGCCTTTG ATCAGAAACT ACCATTTGGC 
TTTCTAAAGG GTGAGAACAG TGAATCGCAG GCCTATGACA AAGTTACGGA GAAGGAGTTG 
GTCTTCAAGG CGGAAGAAAA ACTGAAGAAA GAGCCACTTG TGCAGGCATT CGAAGGCGAA 
GAGGACGAGA GCCGGAGTTC CCTGGACATT AAGGGTAAAT TGGAGGACAC CAGAAACGAC 
TCCAAATCTG AGGAGCAGGA CGATATGAAG CAGGAACCCG AAAGGGTTAG CACTCCCGAT 
CAACAGCAGG CCGAGGATGA TAGGAAATCC ATTGACATCA TGAGCACACC ACCGCCCGCA 
GACACACCCT CTGGAGGAGA TGGACCGTTG GACCTTTCCA TCTGCCGCAA ACGCTCGGCT 
GGATCATTCT TTACCGCACC GGCAGAGGAC AACCTTATGC TCCATAGGTT TATGCCCAGA 
TTACACGAAT TCGAAGCCGA AAGGGGGCAG CCGCTGAAAA TGCGCAAATC ACACAGCTCG 
GCGGAGTCGT CCACCTCGCA GAAGTCGCAC AAGGGCAGCA GTCCCACGCC CACGCCCACC 
GCCTCGCCCG GCCTCACTCC TTCGCCGTCG CCACCCACAA GTGCGGGCGG TGAGCTGAGC 
AGCACGTCCG AGGGCGGAGC GGTACCGACA ATGGCGGCAG CAGCGTTCGC AGCGGACCAC 
AGCGCTCTGG CATCGGGGCC AACGCTTCCG CAGACTCATC CCACCTTCCA TCCGCTGTTG 
CTGGAGGAGA TCTACCGCTC GGGGTTTCCT TTTTTATGCC AACCGGGCGG ACGGCGCGGC 
ATTGAGGCGC TGCTGGCGGG AGCCGCCAGT GCAGCTGCCC CAAAGCGGCC GCTTCCGCCC 
GTGAAGTTCT CAGCGGGCAG TGTGGTGGGA CTCAAGACGA AGGATCGCTA CAGCTGCAAG 
TTCTGCGGCA AGGTGTTCCC CAGATCGGCC AATCTGACGA GGCATTTGAG GACGCACACG 
GGCGAGCAGC CGTATCCCTG CAAGTACTGC GATCGGGCAT TCAGTATATC CTCGAACCTG 
CAGCGCCATG TGAGGAACAT CCACAACAAG GAGCGTCCTT TTCGCTGCGA GCTCTGCGAT 
AGATCCTTTG GCCAGCAGAC GAATCTCGAT CGGCACGTAA AGAAGCACGA GTCGGAGGGC 
AACAATTTCC GGGATTCGCC CAGTTCCAGT GGCATTGCCG AAAGGGAGGA GTACTTCGAT 
GACATAAGGA AGTTCATGAA CCGAGTCTAC ACGCCCAACA GTCTGGCCGG CAATGAGGGC 
GATACCGAGG AGTATCCCAA TAGCGACGAT CAGTCCGTCA ATCTGGAAAA GGACAGTGGC 
AACTTCAACA ACAACAGCAG CAACATCAGC AACAACAACA GCAGCAGCGG CAACAACAAC 
AACAGCTCCA AGGCCATTAC AATAAGCTCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724130.3
CDS	651..3623
Translation	MDAFFKDRAQAEHILQEWVRRREPVCELDIRDSGGVYAKTPLQRGTRYGPFPMKLSHQPNDPQLAWKAHSRHYNGWLEPTEDVSTWLKKIRSVQDDCIGEANLQSYINAGYLWYETNRYVNAGSEMVVDGRPKSPVQLNEDFMNGGKVLAAAAAAAAAVVAASSSGAKSGGGGSSAPSDDRSDRDNGSLYSGDEFSKDKKNSSLIREGDIDFTDDENGFDIRCEVCDKVYPDLDLLDDHLIGAHHFKQDEFPCKQCALRFCHRPLLIKHEAISHNNIRKYSCENCSKVFCDPSNLQRHIRAYHVGARCHPCPECGKTFGTSSGLKQHQHIHSSVKPFACEVCSKAYTQFSNLCRHKRMHATCRMQIKCDKCNQSFSTLTSLTKHKKFCDSTGPGPYRNQHVNRHHQHPHQHPLPHQPHLAATATSTCPAPPRESSESSSSAAAAAVAAMSTPPNPFLMFRTAPSFFPGFPPYGFPPFLPQNPLHPTNIPMFFSKNPMDLGCGGPEITSPVSAFDQKLPFGFLKGENSESQAYDKVTEKELVFKAEEKLKKEPLVQAFEGEEDESRSSLDIKGKLEDTRNDSKSEEQDDMKQEPERVSTPDQQQAEDDRKSIDIMSTPPPADTPSGGDGPLDLSICRKRSAGSFFTAPAEDNLMLHRFMPRLHEFEAERGQPLKMRKSHSSAESSTSQKSHKGSSPTPTPTASPGLTPSPSPPTSAGGELSSTSEGGAVPTMAAAAFAADHSALASGPTLPQTHPTFHPLLLEEIYRSGFPFLCQPGGRRGIEALLAGAASAAAPKRPLPPVKFSAGSVVGLKTKDRYSCKFCGKVFPRSANLTRHLRTHTGEQPYPCKYCDRAFSISSNLQRHVRNIHNKERPFRCELCDRSFGQQTNLDRHVKKHESEGNNFRDSPSSSGIAEREEYFDDIRKFMNRVYTPNSLAGNEGDTEEYPNSDDQSVNLEKDSGNFNNNSSNISNNNSSSGNNNNSSKAITISS

Target ORF information:

RefSeq Version	NM_165262.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster hamlet (ham), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165262.3

ORF Insert Sequence:

ATGGATGCCT TCTTCAAGGA CCGCGCCCAG GCGGAGCACA TCCTGCAGGA GTGGGTGCGT 
AGGCGCGAAC CAGTCTGTGA GCTGGACATC CGTGATAGTG GTGGCGTCTA CGCCAAGACG 
CCGCTCCAGA GGGGCACTCG CTACGGACCC TTTCCCATGA AGCTCAGCCA CCAACCCAAC 
GATCCTCAGC TGGCCTGGAA GGCCCATAGT AGACACTATA ATGGATGGCT AGAGCCCACA 
GAAGATGTGT CCACGTGGTT GAAAAAAATC CGAAGCGTTC AGGACGATTG TATAGGCGAG 
GCTAATTTAC AGAGTTACAT TAATGCTGGT TATCTGTGGT ACGAGACGAA TCGGTACGTG 
AATGCTGGCA GTGAAATGGT CGTCGACGGG CGACCCAAGA GTCCTGTTCA ACTGAACGAG 
GACTTCATGA ACGGCGGCAA GGTCCTGGCA GCAGCCGCAG CAGCAGCCGC CGCAGTGGTG 
GCCGCCTCCT CCTCGGGAGC AAAAAGCGGC GGTGGGGGCA GCTCCGCGCC CAGCGATGAT 
CGCAGCGATC GTGATAATGG TTCTCTGTAC AGTGGTGACG AATTCTCCAA AGATAAGAAA 
AACTCCTCGT TAATCAGAGA GGGCGACATC GATTTCACAG ACGACGAAAA CGGTTTCGAT 
ATACGCTGTG AGGTCTGTGA TAAAGTCTAT CCGGATTTAG ATCTCTTGGA CGATCATCTG 
ATAGGCGCTC ACCATTTCAA GCAGGACGAA TTTCCGTGCA AGCAATGCGC TCTGCGATTT 
TGCCATCGTC CTTTGCTAAT TAAACACGAA GCTATCTCCC ACAATAACAT ACGCAAATAT 
TCCTGCGAAA ATTGCAGCAA GGTATTTTGT GACCCCTCGA ATCTACAGCG GCATATTCGC 
GCCTACCACG TCGGAGCTCG CTGTCATCCG TGTCCGGAGT GCGGGAAAAC GTTCGGAACC 
TCGTCGGGCC TGAAGCAGCA CCAGCACATC CACAGCTCCG TGAAGCCCTT CGCCTGCGAG 
GTGTGCTCCA AGGCGTATAC GCAATTCTCG AATCTGTGCC GTCACAAGCG GATGCACGCC 
ACCTGTCGGA TGCAGATCAA GTGCGACAAG TGCAACCAGA GCTTCAGCAC GTTGACCTCC 
CTGACCAAGC ACAAAAAGTT CTGTGATTCA ACGGGTCCGG GTCCGTATCG AAACCAGCAC 
GTGAATCGCC ACCATCAGCA TCCGCACCAG CACCCACTTC CGCATCAGCC CCACCTGGCC 
GCCACTGCCA CATCCACCTG TCCAGCGCCA CCAAGAGAAT CTTCGGAGTC CTCGTCCTCA 
GCAGCAGCTG CCGCCGTTGC CGCCATGTCG ACGCCACCGA ATCCTTTCCT AATGTTCCGC 
ACTGCGCCGT CTTTCTTTCC CGGATTTCCG CCTTACGGAT TTCCCCCCTT TCTACCACAA 
AATCCGCTGC ATCCTACGAA CATTCCGATG TTTTTCTCGA AAAATCCCAT GGATTTGGGT 
TGCGGTGGTC CGGAGATCAC TTCTCCCGTT TCCGCCTTTG ATCAGAAACT ACCATTTGGC 
TTTCTAAAGG GTGAGAACAG TGAATCGCAG GCCTATGACA AAGTTACGGA GAAGGAGTTG 
GTCTTCAAGG CGGAAGAAAA ACTGAAGAAA GAGCCACTTG TGCAGGCATT CGAAGGCGAA 
GAGGACGAGA GCCGGAGTTC CCTGGACATT AAGGGTAAAT TGGAGGACAC CAGAAACGAC 
TCCAAATCTG AGGAGCAGGA CGATATGAAG CAGGAACCCG AAAGGGTTAG CACTCCCGAT 
CAACAGCAGG CCGAGGATGA TAGGAAATCC ATTGACATCA TGAGCACACC ACCGCCCGCA 
GACACACCCT CTGGAGGAGA TGGACCGTTG GACCTTTCCA TCTGCCGCAA ACGCTCGGCT 
GGATCATTCT TTACCGCACC GGCAGAGGAC AACCTTATGC TCCATAGGTT TATGCCCAGA 
TTACACGAAT TCGAAGCCGA AAGGGGGCAG CCGCTGAAAA TGCGCAAATC ACACAGCTCG 
GCGGAGTCGT CCACCTCGCA GAAGTCGCAC AAGGGCAGCA GTCCCACGCC CACGCCCACC 
GCCTCGCCCG GCCTCACTCC TTCGCCGTCG CCACCCACAA GTGCGGGCGG TGAGCTGAGC 
AGCACGTCCG AGGGCGGAGC GGTACCGACA ATGGCGGCAG CAGCGTTCGC AGCGGACCAC 
AGCGCTCTGG CATCGGGGCC AACGCTTCCG CAGACTCATC CCACCTTCCA TCCGCTGTTG 
CTGGAGGAGA TCTACCGCTC GGGGTTTCCT TTTTTATGCC AACCGGGCGG ACGGCGCGGC 
ATTGAGGCGC TGCTGGCGGG AGCCGCCAGT GCAGCTGCCC CAAAGCGGCC GCTTCCGCCC 
GTGAAGTTCT CAGCGGGCAG TGTGGTGGGA CTCAAGACGA AGGATCGCTA CAGCTGCAAG 
TTCTGCGGCA AGGTGTTCCC CAGATCGGCC AATCTGACGA GGCATTTGAG GACGCACACG 
GGCGAGCAGC CGTATCCCTG CAAGTACTGC GATCGGGCAT TCAGTATATC CTCGAACCTG 
CAGCGCCATG TGAGGAACAT CCACAACAAG GAGCGTCCTT TTCGCTGCGA GCTCTGCGAT 
AGATCCTTTG GCCAGCAGAC GAATCTCGAT CGGCACGTAA AGAAGCACGA GTCGGAGGGC 
AACAATTTCC GGGATTCGCC CAGTTCCAGT GGCATTGCCG AAAGGGAGGA GTACTTCGAT 
GACATAAGGA AGTTCATGAA CCGAGTCTAC ACGCCCAACA GTCTGGCCGG CAATGAGGGC 
GATACCGAGG AGTATCCCAA TAGCGACGAT CAGTCCGTCA ATCTGGAAAA GGACAGTGGC 
AACTTCAACA ACAACAGCAG CAACATCAGC AACAACAACA GCAGCAGCGG CAACAACAAC 
AACAGCTCCA AGGCCATTAC AATAAGCTCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ham ( NM_001259152.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246081.1 Drosophila melanogaster hamlet (ham), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa14923
Clone ID Related Accession (Same CDS sequence)	NM_165262.3 , NM_001259152.1 , NM_001273614.1
Accession Version	NM_001259152.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2973bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	hamlet, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGCCT TCTTCAAGGA CCGCGCCCAG GCGGAGCACA TCCTGCAGGA GTGGGTGCGT 
AGGCGCGAAC CAGTCTGTGA GCTGGACATC CGTGATAGTG GTGGCGTCTA CGCCAAGACG 
CCGCTCCAGA GGGGCACTCG CTACGGACCC TTTCCCATGA AGCTCAGCCA CCAACCCAAC 
GATCCTCAGC TGGCCTGGAA GGCCCATAGT AGACACTATA ATGGATGGCT AGAGCCCACA 
GAAGATGTGT CCACGTGGTT GAAAAAAATC CGAAGCGTTC AGGACGATTG TATAGGCGAG 
GCTAATTTAC AGAGTTACAT TAATGCTGGT TATCTGTGGT ACGAGACGAA TCGGTACGTG 
AATGCTGGCA GTGAAATGGT CGTCGACGGG CGACCCAAGA GTCCTGTTCA ACTGAACGAG 
GACTTCATGA ACGGCGGCAA GGTCCTGGCA GCAGCCGCAG CAGCAGCCGC CGCAGTGGTG 
GCCGCCTCCT CCTCGGGAGC AAAAAGCGGC GGTGGGGGCA GCTCCGCGCC CAGCGATGAT 
CGCAGCGATC GTGATAATGG TTCTCTGTAC AGTGGTGACG AATTCTCCAA AGATAAGAAA 
AACTCCTCGT TAATCAGAGA GGGCGACATC GATTTCACAG ACGACGAAAA CGGTTTCGAT 
ATACGCTGTG AGGTCTGTGA TAAAGTCTAT CCGGATTTAG ATCTCTTGGA CGATCATCTG 
ATAGGCGCTC ACCATTTCAA GCAGGACGAA TTTCCGTGCA AGCAATGCGC TCTGCGATTT 
TGCCATCGTC CTTTGCTAAT TAAACACGAA GCTATCTCCC ACAATAACAT ACGCAAATAT 
TCCTGCGAAA ATTGCAGCAA GGTATTTTGT GACCCCTCGA ATCTACAGCG GCATATTCGC 
GCCTACCACG TCGGAGCTCG CTGTCATCCG TGTCCGGAGT GCGGGAAAAC GTTCGGAACC 
TCGTCGGGCC TGAAGCAGCA CCAGCACATC CACAGCTCCG TGAAGCCCTT CGCCTGCGAG 
GTGTGCTCCA AGGCGTATAC GCAATTCTCG AATCTGTGCC GTCACAAGCG GATGCACGCC 
ACCTGTCGGA TGCAGATCAA GTGCGACAAG TGCAACCAGA GCTTCAGCAC GTTGACCTCC 
CTGACCAAGC ACAAAAAGTT CTGTGATTCA ACGGGTCCGG GTCCGTATCG AAACCAGCAC 
GTGAATCGCC ACCATCAGCA TCCGCACCAG CACCCACTTC CGCATCAGCC CCACCTGGCC 
GCCACTGCCA CATCCACCTG TCCAGCGCCA CCAAGAGAAT CTTCGGAGTC CTCGTCCTCA 
GCAGCAGCTG CCGCCGTTGC CGCCATGTCG ACGCCACCGA ATCCTTTCCT AATGTTCCGC 
ACTGCGCCGT CTTTCTTTCC CGGATTTCCG CCTTACGGAT TTCCCCCCTT TCTACCACAA 
AATCCGCTGC ATCCTACGAA CATTCCGATG TTTTTCTCGA AAAATCCCAT GGATTTGGGT 
TGCGGTGGTC CGGAGATCAC TTCTCCCGTT TCCGCCTTTG ATCAGAAACT ACCATTTGGC 
TTTCTAAAGG GTGAGAACAG TGAATCGCAG GCCTATGACA AAGTTACGGA GAAGGAGTTG 
GTCTTCAAGG CGGAAGAAAA ACTGAAGAAA GAGCCACTTG TGCAGGCATT CGAAGGCGAA 
GAGGACGAGA GCCGGAGTTC CCTGGACATT AAGGGTAAAT TGGAGGACAC CAGAAACGAC 
TCCAAATCTG AGGAGCAGGA CGATATGAAG CAGGAACCCG AAAGGGTTAG CACTCCCGAT 
CAACAGCAGG CCGAGGATGA TAGGAAATCC ATTGACATCA TGAGCACACC ACCGCCCGCA 
GACACACCCT CTGGAGGAGA TGGACCGTTG GACCTTTCCA TCTGCCGCAA ACGCTCGGCT 
GGATCATTCT TTACCGCACC GGCAGAGGAC AACCTTATGC TCCATAGGTT TATGCCCAGA 
TTACACGAAT TCGAAGCCGA AAGGGGGCAG CCGCTGAAAA TGCGCAAATC ACACAGCTCG 
GCGGAGTCGT CCACCTCGCA GAAGTCGCAC AAGGGCAGCA GTCCCACGCC CACGCCCACC 
GCCTCGCCCG GCCTCACTCC TTCGCCGTCG CCACCCACAA GTGCGGGCGG TGAGCTGAGC 
AGCACGTCCG AGGGCGGAGC GGTACCGACA ATGGCGGCAG CAGCGTTCGC AGCGGACCAC 
AGCGCTCTGG CATCGGGGCC AACGCTTCCG CAGACTCATC CCACCTTCCA TCCGCTGTTG 
CTGGAGGAGA TCTACCGCTC GGGGTTTCCT TTTTTATGCC AACCGGGCGG ACGGCGCGGC 
ATTGAGGCGC TGCTGGCGGG AGCCGCCAGT GCAGCTGCCC CAAAGCGGCC GCTTCCGCCC 
GTGAAGTTCT CAGCGGGCAG TGTGGTGGGA CTCAAGACGA AGGATCGCTA CAGCTGCAAG 
TTCTGCGGCA AGGTGTTCCC CAGATCGGCC AATCTGACGA GGCATTTGAG GACGCACACG 
GGCGAGCAGC CGTATCCCTG CAAGTACTGC GATCGGGCAT TCAGTATATC CTCGAACCTG 
CAGCGCCATG TGAGGAACAT CCACAACAAG GAGCGTCCTT TTCGCTGCGA GCTCTGCGAT 
AGATCCTTTG GCCAGCAGAC GAATCTCGAT CGGCACGTAA AGAAGCACGA GTCGGAGGGC 
AACAATTTCC GGGATTCGCC CAGTTCCAGT GGCATTGCCG AAAGGGAGGA GTACTTCGAT 
GACATAAGGA AGTTCATGAA CCGAGTCTAC ACGCCCAACA GTCTGGCCGG CAATGAGGGC 
GATACCGAGG AGTATCCCAA TAGCGACGAT CAGTCCGTCA ATCTGGAAAA GGACAGTGGC 
AACTTCAACA ACAACAGCAG CAACATCAGC AACAACAACA GCAGCAGCGG CAACAACAAC 
AACAGCTCCA AGGCCATTAC AATAAGCTCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246081.1
CDS	650..3622
Translation	MDAFFKDRAQAEHILQEWVRRREPVCELDIRDSGGVYAKTPLQRGTRYGPFPMKLSHQPNDPQLAWKAHSRHYNGWLEPTEDVSTWLKKIRSVQDDCIGEANLQSYINAGYLWYETNRYVNAGSEMVVDGRPKSPVQLNEDFMNGGKVLAAAAAAAAAVVAASSSGAKSGGGGSSAPSDDRSDRDNGSLYSGDEFSKDKKNSSLIREGDIDFTDDENGFDIRCEVCDKVYPDLDLLDDHLIGAHHFKQDEFPCKQCALRFCHRPLLIKHEAISHNNIRKYSCENCSKVFCDPSNLQRHIRAYHVGARCHPCPECGKTFGTSSGLKQHQHIHSSVKPFACEVCSKAYTQFSNLCRHKRMHATCRMQIKCDKCNQSFSTLTSLTKHKKFCDSTGPGPYRNQHVNRHHQHPHQHPLPHQPHLAATATSTCPAPPRESSESSSSAAAAAVAAMSTPPNPFLMFRTAPSFFPGFPPYGFPPFLPQNPLHPTNIPMFFSKNPMDLGCGGPEITSPVSAFDQKLPFGFLKGENSESQAYDKVTEKELVFKAEEKLKKEPLVQAFEGEEDESRSSLDIKGKLEDTRNDSKSEEQDDMKQEPERVSTPDQQQAEDDRKSIDIMSTPPPADTPSGGDGPLDLSICRKRSAGSFFTAPAEDNLMLHRFMPRLHEFEAERGQPLKMRKSHSSAESSTSQKSHKGSSPTPTPTASPGLTPSPSPPTSAGGELSSTSEGGAVPTMAAAAFAADHSALASGPTLPQTHPTFHPLLLEEIYRSGFPFLCQPGGRRGIEALLAGAASAAAPKRPLPPVKFSAGSVVGLKTKDRYSCKFCGKVFPRSANLTRHLRTHTGEQPYPCKYCDRAFSISSNLQRHVRNIHNKERPFRCELCDRSFGQQTNLDRHVKKHESEGNNFRDSPSSSGIAEREEYFDDIRKFMNRVYTPNSLAGNEGDTEEYPNSDDQSVNLEKDSGNFNNNSSNISNNNSSSGNNNNSSKAITISS

Target ORF information:

RefSeq Version	NM_001259152.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster hamlet (ham), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259152.1

ORF Insert Sequence:

ATGGATGCCT TCTTCAAGGA CCGCGCCCAG GCGGAGCACA TCCTGCAGGA GTGGGTGCGT 
AGGCGCGAAC CAGTCTGTGA GCTGGACATC CGTGATAGTG GTGGCGTCTA CGCCAAGACG 
CCGCTCCAGA GGGGCACTCG CTACGGACCC TTTCCCATGA AGCTCAGCCA CCAACCCAAC 
GATCCTCAGC TGGCCTGGAA GGCCCATAGT AGACACTATA ATGGATGGCT AGAGCCCACA 
GAAGATGTGT CCACGTGGTT GAAAAAAATC CGAAGCGTTC AGGACGATTG TATAGGCGAG 
GCTAATTTAC AGAGTTACAT TAATGCTGGT TATCTGTGGT ACGAGACGAA TCGGTACGTG 
AATGCTGGCA GTGAAATGGT CGTCGACGGG CGACCCAAGA GTCCTGTTCA ACTGAACGAG 
GACTTCATGA ACGGCGGCAA GGTCCTGGCA GCAGCCGCAG CAGCAGCCGC CGCAGTGGTG 
GCCGCCTCCT CCTCGGGAGC AAAAAGCGGC GGTGGGGGCA GCTCCGCGCC CAGCGATGAT 
CGCAGCGATC GTGATAATGG TTCTCTGTAC AGTGGTGACG AATTCTCCAA AGATAAGAAA 
AACTCCTCGT TAATCAGAGA GGGCGACATC GATTTCACAG ACGACGAAAA CGGTTTCGAT 
ATACGCTGTG AGGTCTGTGA TAAAGTCTAT CCGGATTTAG ATCTCTTGGA CGATCATCTG 
ATAGGCGCTC ACCATTTCAA GCAGGACGAA TTTCCGTGCA AGCAATGCGC TCTGCGATTT 
TGCCATCGTC CTTTGCTAAT TAAACACGAA GCTATCTCCC ACAATAACAT ACGCAAATAT 
TCCTGCGAAA ATTGCAGCAA GGTATTTTGT GACCCCTCGA ATCTACAGCG GCATATTCGC 
GCCTACCACG TCGGAGCTCG CTGTCATCCG TGTCCGGAGT GCGGGAAAAC GTTCGGAACC 
TCGTCGGGCC TGAAGCAGCA CCAGCACATC CACAGCTCCG TGAAGCCCTT CGCCTGCGAG 
GTGTGCTCCA AGGCGTATAC GCAATTCTCG AATCTGTGCC GTCACAAGCG GATGCACGCC 
ACCTGTCGGA TGCAGATCAA GTGCGACAAG TGCAACCAGA GCTTCAGCAC GTTGACCTCC 
CTGACCAAGC ACAAAAAGTT CTGTGATTCA ACGGGTCCGG GTCCGTATCG AAACCAGCAC 
GTGAATCGCC ACCATCAGCA TCCGCACCAG CACCCACTTC CGCATCAGCC CCACCTGGCC 
GCCACTGCCA CATCCACCTG TCCAGCGCCA CCAAGAGAAT CTTCGGAGTC CTCGTCCTCA 
GCAGCAGCTG CCGCCGTTGC CGCCATGTCG ACGCCACCGA ATCCTTTCCT AATGTTCCGC 
ACTGCGCCGT CTTTCTTTCC CGGATTTCCG CCTTACGGAT TTCCCCCCTT TCTACCACAA 
AATCCGCTGC ATCCTACGAA CATTCCGATG TTTTTCTCGA AAAATCCCAT GGATTTGGGT 
TGCGGTGGTC CGGAGATCAC TTCTCCCGTT TCCGCCTTTG ATCAGAAACT ACCATTTGGC 
TTTCTAAAGG GTGAGAACAG TGAATCGCAG GCCTATGACA AAGTTACGGA GAAGGAGTTG 
GTCTTCAAGG CGGAAGAAAA ACTGAAGAAA GAGCCACTTG TGCAGGCATT CGAAGGCGAA 
GAGGACGAGA GCCGGAGTTC CCTGGACATT AAGGGTAAAT TGGAGGACAC CAGAAACGAC 
TCCAAATCTG AGGAGCAGGA CGATATGAAG CAGGAACCCG AAAGGGTTAG CACTCCCGAT 
CAACAGCAGG CCGAGGATGA TAGGAAATCC ATTGACATCA TGAGCACACC ACCGCCCGCA 
GACACACCCT CTGGAGGAGA TGGACCGTTG GACCTTTCCA TCTGCCGCAA ACGCTCGGCT 
GGATCATTCT TTACCGCACC GGCAGAGGAC AACCTTATGC TCCATAGGTT TATGCCCAGA 
TTACACGAAT TCGAAGCCGA AAGGGGGCAG CCGCTGAAAA TGCGCAAATC ACACAGCTCG 
GCGGAGTCGT CCACCTCGCA GAAGTCGCAC AAGGGCAGCA GTCCCACGCC CACGCCCACC 
GCCTCGCCCG GCCTCACTCC TTCGCCGTCG CCACCCACAA GTGCGGGCGG TGAGCTGAGC 
AGCACGTCCG AGGGCGGAGC GGTACCGACA ATGGCGGCAG CAGCGTTCGC AGCGGACCAC 
AGCGCTCTGG CATCGGGGCC AACGCTTCCG CAGACTCATC CCACCTTCCA TCCGCTGTTG 
CTGGAGGAGA TCTACCGCTC GGGGTTTCCT TTTTTATGCC AACCGGGCGG ACGGCGCGGC 
ATTGAGGCGC TGCTGGCGGG AGCCGCCAGT GCAGCTGCCC CAAAGCGGCC GCTTCCGCCC 
GTGAAGTTCT CAGCGGGCAG TGTGGTGGGA CTCAAGACGA AGGATCGCTA CAGCTGCAAG 
TTCTGCGGCA AGGTGTTCCC CAGATCGGCC AATCTGACGA GGCATTTGAG GACGCACACG 
GGCGAGCAGC CGTATCCCTG CAAGTACTGC GATCGGGCAT TCAGTATATC CTCGAACCTG 
CAGCGCCATG TGAGGAACAT CCACAACAAG GAGCGTCCTT TTCGCTGCGA GCTCTGCGAT 
AGATCCTTTG GCCAGCAGAC GAATCTCGAT CGGCACGTAA AGAAGCACGA GTCGGAGGGC 
AACAATTTCC GGGATTCGCC CAGTTCCAGT GGCATTGCCG AAAGGGAGGA GTACTTCGAT 
GACATAAGGA AGTTCATGAA CCGAGTCTAC ACGCCCAACA GTCTGGCCGG CAATGAGGGC 
GATACCGAGG AGTATCCCAA TAGCGACGAT CAGTCCGTCA ATCTGGAAAA GGACAGTGGC 
AACTTCAACA ACAACAGCAG CAACATCAGC AACAACAACA GCAGCAGCGG CAACAACAAC 
AACAGCTCCA AGGCCATTAC AATAAGCTCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ham ( NM_001273614.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260543.1 Drosophila melanogaster hamlet (ham), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa14923
Clone ID Related Accession (Same CDS sequence)	NM_165262.3 , NM_001259152.1 , NM_001273614.1
Accession Version	NM_001273614.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2973bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	hamlet, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGCCT TCTTCAAGGA CCGCGCCCAG GCGGAGCACA TCCTGCAGGA GTGGGTGCGT 
AGGCGCGAAC CAGTCTGTGA GCTGGACATC CGTGATAGTG GTGGCGTCTA CGCCAAGACG 
CCGCTCCAGA GGGGCACTCG CTACGGACCC TTTCCCATGA AGCTCAGCCA CCAACCCAAC 
GATCCTCAGC TGGCCTGGAA GGCCCATAGT AGACACTATA ATGGATGGCT AGAGCCCACA 
GAAGATGTGT CCACGTGGTT GAAAAAAATC CGAAGCGTTC AGGACGATTG TATAGGCGAG 
GCTAATTTAC AGAGTTACAT TAATGCTGGT TATCTGTGGT ACGAGACGAA TCGGTACGTG 
AATGCTGGCA GTGAAATGGT CGTCGACGGG CGACCCAAGA GTCCTGTTCA ACTGAACGAG 
GACTTCATGA ACGGCGGCAA GGTCCTGGCA GCAGCCGCAG CAGCAGCCGC CGCAGTGGTG 
GCCGCCTCCT CCTCGGGAGC AAAAAGCGGC GGTGGGGGCA GCTCCGCGCC CAGCGATGAT 
CGCAGCGATC GTGATAATGG TTCTCTGTAC AGTGGTGACG AATTCTCCAA AGATAAGAAA 
AACTCCTCGT TAATCAGAGA GGGCGACATC GATTTCACAG ACGACGAAAA CGGTTTCGAT 
ATACGCTGTG AGGTCTGTGA TAAAGTCTAT CCGGATTTAG ATCTCTTGGA CGATCATCTG 
ATAGGCGCTC ACCATTTCAA GCAGGACGAA TTTCCGTGCA AGCAATGCGC TCTGCGATTT 
TGCCATCGTC CTTTGCTAAT TAAACACGAA GCTATCTCCC ACAATAACAT ACGCAAATAT 
TCCTGCGAAA ATTGCAGCAA GGTATTTTGT GACCCCTCGA ATCTACAGCG GCATATTCGC 
GCCTACCACG TCGGAGCTCG CTGTCATCCG TGTCCGGAGT GCGGGAAAAC GTTCGGAACC 
TCGTCGGGCC TGAAGCAGCA CCAGCACATC CACAGCTCCG TGAAGCCCTT CGCCTGCGAG 
GTGTGCTCCA AGGCGTATAC GCAATTCTCG AATCTGTGCC GTCACAAGCG GATGCACGCC 
ACCTGTCGGA TGCAGATCAA GTGCGACAAG TGCAACCAGA GCTTCAGCAC GTTGACCTCC 
CTGACCAAGC ACAAAAAGTT CTGTGATTCA ACGGGTCCGG GTCCGTATCG AAACCAGCAC 
GTGAATCGCC ACCATCAGCA TCCGCACCAG CACCCACTTC CGCATCAGCC CCACCTGGCC 
GCCACTGCCA CATCCACCTG TCCAGCGCCA CCAAGAGAAT CTTCGGAGTC CTCGTCCTCA 
GCAGCAGCTG CCGCCGTTGC CGCCATGTCG ACGCCACCGA ATCCTTTCCT AATGTTCCGC 
ACTGCGCCGT CTTTCTTTCC CGGATTTCCG CCTTACGGAT TTCCCCCCTT TCTACCACAA 
AATCCGCTGC ATCCTACGAA CATTCCGATG TTTTTCTCGA AAAATCCCAT GGATTTGGGT 
TGCGGTGGTC CGGAGATCAC TTCTCCCGTT TCCGCCTTTG ATCAGAAACT ACCATTTGGC 
TTTCTAAAGG GTGAGAACAG TGAATCGCAG GCCTATGACA AAGTTACGGA GAAGGAGTTG 
GTCTTCAAGG CGGAAGAAAA ACTGAAGAAA GAGCCACTTG TGCAGGCATT CGAAGGCGAA 
GAGGACGAGA GCCGGAGTTC CCTGGACATT AAGGGTAAAT TGGAGGACAC CAGAAACGAC 
TCCAAATCTG AGGAGCAGGA CGATATGAAG CAGGAACCCG AAAGGGTTAG CACTCCCGAT 
CAACAGCAGG CCGAGGATGA TAGGAAATCC ATTGACATCA TGAGCACACC ACCGCCCGCA 
GACACACCCT CTGGAGGAGA TGGACCGTTG GACCTTTCCA TCTGCCGCAA ACGCTCGGCT 
GGATCATTCT TTACCGCACC GGCAGAGGAC AACCTTATGC TCCATAGGTT TATGCCCAGA 
TTACACGAAT TCGAAGCCGA AAGGGGGCAG CCGCTGAAAA TGCGCAAATC ACACAGCTCG 
GCGGAGTCGT CCACCTCGCA GAAGTCGCAC AAGGGCAGCA GTCCCACGCC CACGCCCACC 
GCCTCGCCCG GCCTCACTCC TTCGCCGTCG CCACCCACAA GTGCGGGCGG TGAGCTGAGC 
AGCACGTCCG AGGGCGGAGC GGTACCGACA ATGGCGGCAG CAGCGTTCGC AGCGGACCAC 
AGCGCTCTGG CATCGGGGCC AACGCTTCCG CAGACTCATC CCACCTTCCA TCCGCTGTTG 
CTGGAGGAGA TCTACCGCTC GGGGTTTCCT TTTTTATGCC AACCGGGCGG ACGGCGCGGC 
ATTGAGGCGC TGCTGGCGGG AGCCGCCAGT GCAGCTGCCC CAAAGCGGCC GCTTCCGCCC 
GTGAAGTTCT CAGCGGGCAG TGTGGTGGGA CTCAAGACGA AGGATCGCTA CAGCTGCAAG 
TTCTGCGGCA AGGTGTTCCC CAGATCGGCC AATCTGACGA GGCATTTGAG GACGCACACG 
GGCGAGCAGC CGTATCCCTG CAAGTACTGC GATCGGGCAT TCAGTATATC CTCGAACCTG 
CAGCGCCATG TGAGGAACAT CCACAACAAG GAGCGTCCTT TTCGCTGCGA GCTCTGCGAT 
AGATCCTTTG GCCAGCAGAC GAATCTCGAT CGGCACGTAA AGAAGCACGA GTCGGAGGGC 
AACAATTTCC GGGATTCGCC CAGTTCCAGT GGCATTGCCG AAAGGGAGGA GTACTTCGAT 
GACATAAGGA AGTTCATGAA CCGAGTCTAC ACGCCCAACA GTCTGGCCGG CAATGAGGGC 
GATACCGAGG AGTATCCCAA TAGCGACGAT CAGTCCGTCA ATCTGGAAAA GGACAGTGGC 
AACTTCAACA ACAACAGCAG CAACATCAGC AACAACAACA GCAGCAGCGG CAACAACAAC 
AACAGCTCCA AGGCCATTAC AATAAGCTCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260543.1
CDS	982..3954
Translation	MDAFFKDRAQAEHILQEWVRRREPVCELDIRDSGGVYAKTPLQRGTRYGPFPMKLSHQPNDPQLAWKAHSRHYNGWLEPTEDVSTWLKKIRSVQDDCIGEANLQSYINAGYLWYETNRYVNAGSEMVVDGRPKSPVQLNEDFMNGGKVLAAAAAAAAAVVAASSSGAKSGGGGSSAPSDDRSDRDNGSLYSGDEFSKDKKNSSLIREGDIDFTDDENGFDIRCEVCDKVYPDLDLLDDHLIGAHHFKQDEFPCKQCALRFCHRPLLIKHEAISHNNIRKYSCENCSKVFCDPSNLQRHIRAYHVGARCHPCPECGKTFGTSSGLKQHQHIHSSVKPFACEVCSKAYTQFSNLCRHKRMHATCRMQIKCDKCNQSFSTLTSLTKHKKFCDSTGPGPYRNQHVNRHHQHPHQHPLPHQPHLAATATSTCPAPPRESSESSSSAAAAAVAAMSTPPNPFLMFRTAPSFFPGFPPYGFPPFLPQNPLHPTNIPMFFSKNPMDLGCGGPEITSPVSAFDQKLPFGFLKGENSESQAYDKVTEKELVFKAEEKLKKEPLVQAFEGEEDESRSSLDIKGKLEDTRNDSKSEEQDDMKQEPERVSTPDQQQAEDDRKSIDIMSTPPPADTPSGGDGPLDLSICRKRSAGSFFTAPAEDNLMLHRFMPRLHEFEAERGQPLKMRKSHSSAESSTSQKSHKGSSPTPTPTASPGLTPSPSPPTSAGGELSSTSEGGAVPTMAAAAFAADHSALASGPTLPQTHPTFHPLLLEEIYRSGFPFLCQPGGRRGIEALLAGAASAAAPKRPLPPVKFSAGSVVGLKTKDRYSCKFCGKVFPRSANLTRHLRTHTGEQPYPCKYCDRAFSISSNLQRHVRNIHNKERPFRCELCDRSFGQQTNLDRHVKKHESEGNNFRDSPSSSGIAEREEYFDDIRKFMNRVYTPNSLAGNEGDTEEYPNSDDQSVNLEKDSGNFNNNSSNISNNNSSSGNNNNSSKAITISS

Target ORF information:

RefSeq Version	NM_001273614.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster hamlet (ham), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273614.1

ORF Insert Sequence:

ATGGATGCCT TCTTCAAGGA CCGCGCCCAG GCGGAGCACA TCCTGCAGGA GTGGGTGCGT 
AGGCGCGAAC CAGTCTGTGA GCTGGACATC CGTGATAGTG GTGGCGTCTA CGCCAAGACG 
CCGCTCCAGA GGGGCACTCG CTACGGACCC TTTCCCATGA AGCTCAGCCA CCAACCCAAC 
GATCCTCAGC TGGCCTGGAA GGCCCATAGT AGACACTATA ATGGATGGCT AGAGCCCACA 
GAAGATGTGT CCACGTGGTT GAAAAAAATC CGAAGCGTTC AGGACGATTG TATAGGCGAG 
GCTAATTTAC AGAGTTACAT TAATGCTGGT TATCTGTGGT ACGAGACGAA TCGGTACGTG 
AATGCTGGCA GTGAAATGGT CGTCGACGGG CGACCCAAGA GTCCTGTTCA ACTGAACGAG 
GACTTCATGA ACGGCGGCAA GGTCCTGGCA GCAGCCGCAG CAGCAGCCGC CGCAGTGGTG 
GCCGCCTCCT CCTCGGGAGC AAAAAGCGGC GGTGGGGGCA GCTCCGCGCC CAGCGATGAT 
CGCAGCGATC GTGATAATGG TTCTCTGTAC AGTGGTGACG AATTCTCCAA AGATAAGAAA 
AACTCCTCGT TAATCAGAGA GGGCGACATC GATTTCACAG ACGACGAAAA CGGTTTCGAT 
ATACGCTGTG AGGTCTGTGA TAAAGTCTAT CCGGATTTAG ATCTCTTGGA CGATCATCTG 
ATAGGCGCTC ACCATTTCAA GCAGGACGAA TTTCCGTGCA AGCAATGCGC TCTGCGATTT 
TGCCATCGTC CTTTGCTAAT TAAACACGAA GCTATCTCCC ACAATAACAT ACGCAAATAT 
TCCTGCGAAA ATTGCAGCAA GGTATTTTGT GACCCCTCGA ATCTACAGCG GCATATTCGC 
GCCTACCACG TCGGAGCTCG CTGTCATCCG TGTCCGGAGT GCGGGAAAAC GTTCGGAACC 
TCGTCGGGCC TGAAGCAGCA CCAGCACATC CACAGCTCCG TGAAGCCCTT CGCCTGCGAG 
GTGTGCTCCA AGGCGTATAC GCAATTCTCG AATCTGTGCC GTCACAAGCG GATGCACGCC 
ACCTGTCGGA TGCAGATCAA GTGCGACAAG TGCAACCAGA GCTTCAGCAC GTTGACCTCC 
CTGACCAAGC ACAAAAAGTT CTGTGATTCA ACGGGTCCGG GTCCGTATCG AAACCAGCAC 
GTGAATCGCC ACCATCAGCA TCCGCACCAG CACCCACTTC CGCATCAGCC CCACCTGGCC 
GCCACTGCCA CATCCACCTG TCCAGCGCCA CCAAGAGAAT CTTCGGAGTC CTCGTCCTCA 
GCAGCAGCTG CCGCCGTTGC CGCCATGTCG ACGCCACCGA ATCCTTTCCT AATGTTCCGC 
ACTGCGCCGT CTTTCTTTCC CGGATTTCCG CCTTACGGAT TTCCCCCCTT TCTACCACAA 
AATCCGCTGC ATCCTACGAA CATTCCGATG TTTTTCTCGA AAAATCCCAT GGATTTGGGT 
TGCGGTGGTC CGGAGATCAC TTCTCCCGTT TCCGCCTTTG ATCAGAAACT ACCATTTGGC 
TTTCTAAAGG GTGAGAACAG TGAATCGCAG GCCTATGACA AAGTTACGGA GAAGGAGTTG 
GTCTTCAAGG CGGAAGAAAA ACTGAAGAAA GAGCCACTTG TGCAGGCATT CGAAGGCGAA 
GAGGACGAGA GCCGGAGTTC CCTGGACATT AAGGGTAAAT TGGAGGACAC CAGAAACGAC 
TCCAAATCTG AGGAGCAGGA CGATATGAAG CAGGAACCCG AAAGGGTTAG CACTCCCGAT 
CAACAGCAGG CCGAGGATGA TAGGAAATCC ATTGACATCA TGAGCACACC ACCGCCCGCA 
GACACACCCT CTGGAGGAGA TGGACCGTTG GACCTTTCCA TCTGCCGCAA ACGCTCGGCT 
GGATCATTCT TTACCGCACC GGCAGAGGAC AACCTTATGC TCCATAGGTT TATGCCCAGA 
TTACACGAAT TCGAAGCCGA AAGGGGGCAG CCGCTGAAAA TGCGCAAATC ACACAGCTCG 
GCGGAGTCGT CCACCTCGCA GAAGTCGCAC AAGGGCAGCA GTCCCACGCC CACGCCCACC 
GCCTCGCCCG GCCTCACTCC TTCGCCGTCG CCACCCACAA GTGCGGGCGG TGAGCTGAGC 
AGCACGTCCG AGGGCGGAGC GGTACCGACA ATGGCGGCAG CAGCGTTCGC AGCGGACCAC 
AGCGCTCTGG CATCGGGGCC AACGCTTCCG CAGACTCATC CCACCTTCCA TCCGCTGTTG 
CTGGAGGAGA TCTACCGCTC GGGGTTTCCT TTTTTATGCC AACCGGGCGG ACGGCGCGGC 
ATTGAGGCGC TGCTGGCGGG AGCCGCCAGT GCAGCTGCCC CAAAGCGGCC GCTTCCGCCC 
GTGAAGTTCT CAGCGGGCAG TGTGGTGGGA CTCAAGACGA AGGATCGCTA CAGCTGCAAG 
TTCTGCGGCA AGGTGTTCCC CAGATCGGCC AATCTGACGA GGCATTTGAG GACGCACACG 
GGCGAGCAGC CGTATCCCTG CAAGTACTGC GATCGGGCAT TCAGTATATC CTCGAACCTG 
CAGCGCCATG TGAGGAACAT CCACAACAAG GAGCGTCCTT TTCGCTGCGA GCTCTGCGAT 
AGATCCTTTG GCCAGCAGAC GAATCTCGAT CGGCACGTAA AGAAGCACGA GTCGGAGGGC 
AACAATTTCC GGGATTCGCC CAGTTCCAGT GGCATTGCCG AAAGGGAGGA GTACTTCGAT 
GACATAAGGA AGTTCATGAA CCGAGTCTAC ACGCCCAACA GTCTGGCCGG CAATGAGGGC 
GATACCGAGG AGTATCCCAA TAGCGACGAT CAGTCCGTCA ATCTGGAAAA GGACAGTGGC 
AACTTCAACA ACAACAGCAG CAACATCAGC AACAACAACA GCAGCAGCGG CAACAACAAC 
AACAGCTCCA AGGCCATTAC AATAAGCTCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ham ( NM_001273616.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260545.1 Drosophila melanogaster hamlet (ham), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa16007
Clone ID Related Accession (Same CDS sequence)	NM_001273616.1
Accession Version	NM_001273616.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2841bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	hamlet, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGCCT TCTTCAAGGA CCGCGCCCAG GCGGAGCACA TCCTGCAGGA GTGGGTGCGT 
AGGCGCGAAC CAGTCTGTGA GCTGGACATC CGTGATAGTG GTGGCGTCTA CGCCAAGACG 
CCGCTCCAGA GGGGCACTCG CTACGGACCC TTTCCCATGA AGCTCAGCCA CCAACCCAAC 
GATCCTCAGC TGGCCTGGAA GCTGTGGTAC GAGACGAATC GGTACGTGAA TGCTGGCAGT 
GAAATGGTCG TCGACGGGCG ACCCAAGAGT CCTGTTCAAC TGAACGAGGA CTTCATGAAC 
GGCGGCAAGG TCCTGGCAGC AGCCGCAGCA GCAGCCGCCG CAGTGGTGGC CGCCTCCTCC 
TCGGGAGCAA AAAGCGGCGG TGGGGGCAGC TCCGCGCCCA GCGATGATCG CAGCGATCGT 
GATAATGGTT CTCTGTACAG TGGTGACGAA TTCTCCAAAG ATAAGAAAAA CTCCTCGTTA 
ATCAGAGAGG GCGACATCGA TTTCACAGAC GACGAAAACG GTTTCGATAT ACGCTGTGAG 
GTCTGTGATA AAGTCTATCC GGATTTAGAT CTCTTGGACG ATCATCTGAT AGGCGCTCAC 
CATTTCAAGC AGGACGAATT TCCGTGCAAG CAATGCGCTC TGCGATTTTG CCATCGTCCT 
TTGCTAATTA AACACGAAGC TATCTCCCAC AATAACATAC GCAAATATTC CTGCGAAAAT 
TGCAGCAAGG TATTTTGTGA CCCCTCGAAT CTACAGCGGC ATATTCGCGC CTACCACGTC 
GGAGCTCGCT GTCATCCGTG TCCGGAGTGC GGGAAAACGT TCGGAACCTC GTCGGGCCTG 
AAGCAGCACC AGCACATCCA CAGCTCCGTG AAGCCCTTCG CCTGCGAGGT GTGCTCCAAG 
GCGTATACGC AATTCTCGAA TCTGTGCCGT CACAAGCGGA TGCACGCCAC CTGTCGGATG 
CAGATCAAGT GCGACAAGTG CAACCAGAGC TTCAGCACGT TGACCTCCCT GACCAAGCAC 
AAAAAGTTCT GTGATTCAAC GGGTCCGGGT CCGTATCGAA ACCAGCACGT GAATCGCCAC 
CATCAGCATC CGCACCAGCA CCCACTTCCG CATCAGCCCC ACCTGGCCGC CACTGCCACA 
TCCACCTGTC CAGCGCCACC AAGAGAATCT TCGGAGTCCT CGTCCTCAGC AGCAGCTGCC 
GCCGTTGCCG CCATGTCGAC GCCACCGAAT CCTTTCCTAA TGTTCCGCAC TGCGCCGTCT 
TTCTTTCCCG GATTTCCGCC TTACGGATTT CCCCCCTTTC TACCACAAAA TCCGCTGCAT 
CCTACGAACA TTCCGATGTT TTTCTCGAAA AATCCCATGG ATTTGGGTTG CGGTGGTCCG 
GAGATCACTT CTCCCGTTTC CGCCTTTGAT CAGAAACTAC CATTTGGCTT TCTAAAGGGT 
GAGAACAGTG AATCGCAGGC CTATGACAAA GTTACGGAGA AGGAGTTGGT CTTCAAGGCG 
GAAGAAAAAC TGAAGAAAGA GCCACTTGTG CAGGCATTCG AAGGCGAAGA GGACGAGAGC 
CGGAGTTCCC TGGACATTAA GGGTAAATTG GAGGACACCA GAAACGACTC CAAATCTGAG 
GAGCAGGACG ATATGAAGCA GGAACCCGAA AGGGTTAGCA CTCCCGATCA ACAGCAGGCC 
GAGGATGATA GGAAATCCAT TGACATCATG AGCACACCAC CGCCCGCAGA CACACCCTCT 
GGAGGAGATG GACCGTTGGA CCTTTCCATC TGCCGCAAAC GCTCGGCTGG ATCATTCTTT 
ACCGCACCGG CAGAGGACAA CCTTATGCTC CATAGGTTTA TGCCCAGATT ACACGAATTC 
GAAGCCGAAA GGGGGCAGCC GCTGAAAATG CGCAAATCAC ACAGCTCGGC GGAGTCGTCC 
ACCTCGCAGA AGTCGCACAA GGGCAGCAGT CCCACGCCCA CGCCCACCGC CTCGCCCGGC 
CTCACTCCTT CGCCGTCGCC ACCCACAAGT GCGGGCGGTG AGCTGAGCAG CACGTCCGAG 
GGCGGAGCGG TACCGACAAT GGCGGCAGCA GCGTTCGCAG CGGACCACAG CGCTCTGGCA 
TCGGGGCCAA CGCTTCCGCA GACTCATCCC ACCTTCCATC CGCTGTTGCT GGAGGAGATC 
TACCGCTCGG GGTTTCCTTT TTTATGCCAA CCGGGCGGAC GGCGCGGCAT TGAGGCGCTG 
CTGGCGGGAG CCGCCAGTGC AGCTGCCCCA AAGCGGCCGC TTCCGCCCGT GAAGTTCTCA 
GCGGGCAGTG TGGTGGGACT CAAGACGAAG GATCGCTACA GCTGCAAGTT CTGCGGCAAG 
GTGTTCCCCA GATCGGCCAA TCTGACGAGG CATTTGAGGA CGCACACGGG CGAGCAGCCG 
TATCCCTGCA AGTACTGCGA TCGGGCATTC AGTATATCCT CGAACCTGCA GCGCCATGTG 
AGGAACATCC ACAACAAGGA GCGTCCTTTT CGCTGCGAGC TCTGCGATAG ATCCTTTGGC 
CAGCAGACGA ATCTCGATCG GCACGTAAAG AAGCACGAGT CGGAGGGCAA CAATTTCCGG 
GATTCGCCCA GTTCCAGTGG CATTGCCGAA AGGGAGGAGT ACTTCGATGA CATAAGGAAG 
TTCATGAACC GAGTCTACAC GCCCAACAGT CTGGCCGGCA ATGAGGGCGA TACCGAGGAG 
TATCCCAATA GCGACGATCA GTCCGTCAAT CTGGAAAAGG ACAGTGGCAA CTTCAACAAC 
AACAGCAGCA ACATCAGCAA CAACAACAGC AGCAGCGGCA ACAACAACAA CAGCTCCAAG 
GCCATTACAA TAAGCTCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260545.1
CDS	651..3491
Translation	MDAFFKDRAQAEHILQEWVRRREPVCELDIRDSGGVYAKTPLQRGTRYGPFPMKLSHQPNDPQLAWKLWYETNRYVNAGSEMVVDGRPKSPVQLNEDFMNGGKVLAAAAAAAAAVVAASSSGAKSGGGGSSAPSDDRSDRDNGSLYSGDEFSKDKKNSSLIREGDIDFTDDENGFDIRCEVCDKVYPDLDLLDDHLIGAHHFKQDEFPCKQCALRFCHRPLLIKHEAISHNNIRKYSCENCSKVFCDPSNLQRHIRAYHVGARCHPCPECGKTFGTSSGLKQHQHIHSSVKPFACEVCSKAYTQFSNLCRHKRMHATCRMQIKCDKCNQSFSTLTSLTKHKKFCDSTGPGPYRNQHVNRHHQHPHQHPLPHQPHLAATATSTCPAPPRESSESSSSAAAAAVAAMSTPPNPFLMFRTAPSFFPGFPPYGFPPFLPQNPLHPTNIPMFFSKNPMDLGCGGPEITSPVSAFDQKLPFGFLKGENSESQAYDKVTEKELVFKAEEKLKKEPLVQAFEGEEDESRSSLDIKGKLEDTRNDSKSEEQDDMKQEPERVSTPDQQQAEDDRKSIDIMSTPPPADTPSGGDGPLDLSICRKRSAGSFFTAPAEDNLMLHRFMPRLHEFEAERGQPLKMRKSHSSAESSTSQKSHKGSSPTPTPTASPGLTPSPSPPTSAGGELSSTSEGGAVPTMAAAAFAADHSALASGPTLPQTHPTFHPLLLEEIYRSGFPFLCQPGGRRGIEALLAGAASAAAPKRPLPPVKFSAGSVVGLKTKDRYSCKFCGKVFPRSANLTRHLRTHTGEQPYPCKYCDRAFSISSNLQRHVRNIHNKERPFRCELCDRSFGQQTNLDRHVKKHESEGNNFRDSPSSSGIAEREEYFDDIRKFMNRVYTPNSLAGNEGDTEEYPNSDDQSVNLEKDSGNFNNNSSNISNNNSSSGNNNNSSKAITISS

Target ORF information:

RefSeq Version	NM_001273616.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster hamlet (ham), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273616.1

ORF Insert Sequence:

ATGGATGCCT TCTTCAAGGA CCGCGCCCAG GCGGAGCACA TCCTGCAGGA GTGGGTGCGT 
AGGCGCGAAC CAGTCTGTGA GCTGGACATC CGTGATAGTG GTGGCGTCTA CGCCAAGACG 
CCGCTCCAGA GGGGCACTCG CTACGGACCC TTTCCCATGA AGCTCAGCCA CCAACCCAAC 
GATCCTCAGC TGGCCTGGAA GCTGTGGTAC GAGACGAATC GGTACGTGAA TGCTGGCAGT 
GAAATGGTCG TCGACGGGCG ACCCAAGAGT CCTGTTCAAC TGAACGAGGA CTTCATGAAC 
GGCGGCAAGG TCCTGGCAGC AGCCGCAGCA GCAGCCGCCG CAGTGGTGGC CGCCTCCTCC 
TCGGGAGCAA AAAGCGGCGG TGGGGGCAGC TCCGCGCCCA GCGATGATCG CAGCGATCGT 
GATAATGGTT CTCTGTACAG TGGTGACGAA TTCTCCAAAG ATAAGAAAAA CTCCTCGTTA 
ATCAGAGAGG GCGACATCGA TTTCACAGAC GACGAAAACG GTTTCGATAT ACGCTGTGAG 
GTCTGTGATA AAGTCTATCC GGATTTAGAT CTCTTGGACG ATCATCTGAT AGGCGCTCAC 
CATTTCAAGC AGGACGAATT TCCGTGCAAG CAATGCGCTC TGCGATTTTG CCATCGTCCT 
TTGCTAATTA AACACGAAGC TATCTCCCAC AATAACATAC GCAAATATTC CTGCGAAAAT 
TGCAGCAAGG TATTTTGTGA CCCCTCGAAT CTACAGCGGC ATATTCGCGC CTACCACGTC 
GGAGCTCGCT GTCATCCGTG TCCGGAGTGC GGGAAAACGT TCGGAACCTC GTCGGGCCTG 
AAGCAGCACC AGCACATCCA CAGCTCCGTG AAGCCCTTCG CCTGCGAGGT GTGCTCCAAG 
GCGTATACGC AATTCTCGAA TCTGTGCCGT CACAAGCGGA TGCACGCCAC CTGTCGGATG 
CAGATCAAGT GCGACAAGTG CAACCAGAGC TTCAGCACGT TGACCTCCCT GACCAAGCAC 
AAAAAGTTCT GTGATTCAAC GGGTCCGGGT CCGTATCGAA ACCAGCACGT GAATCGCCAC 
CATCAGCATC CGCACCAGCA CCCACTTCCG CATCAGCCCC ACCTGGCCGC CACTGCCACA 
TCCACCTGTC CAGCGCCACC AAGAGAATCT TCGGAGTCCT CGTCCTCAGC AGCAGCTGCC 
GCCGTTGCCG CCATGTCGAC GCCACCGAAT CCTTTCCTAA TGTTCCGCAC TGCGCCGTCT 
TTCTTTCCCG GATTTCCGCC TTACGGATTT CCCCCCTTTC TACCACAAAA TCCGCTGCAT 
CCTACGAACA TTCCGATGTT TTTCTCGAAA AATCCCATGG ATTTGGGTTG CGGTGGTCCG 
GAGATCACTT CTCCCGTTTC CGCCTTTGAT CAGAAACTAC CATTTGGCTT TCTAAAGGGT 
GAGAACAGTG AATCGCAGGC CTATGACAAA GTTACGGAGA AGGAGTTGGT CTTCAAGGCG 
GAAGAAAAAC TGAAGAAAGA GCCACTTGTG CAGGCATTCG AAGGCGAAGA GGACGAGAGC 
CGGAGTTCCC TGGACATTAA GGGTAAATTG GAGGACACCA GAAACGACTC CAAATCTGAG 
GAGCAGGACG ATATGAAGCA GGAACCCGAA AGGGTTAGCA CTCCCGATCA ACAGCAGGCC 
GAGGATGATA GGAAATCCAT TGACATCATG AGCACACCAC CGCCCGCAGA CACACCCTCT 
GGAGGAGATG GACCGTTGGA CCTTTCCATC TGCCGCAAAC GCTCGGCTGG ATCATTCTTT 
ACCGCACCGG CAGAGGACAA CCTTATGCTC CATAGGTTTA TGCCCAGATT ACACGAATTC 
GAAGCCGAAA GGGGGCAGCC GCTGAAAATG CGCAAATCAC ACAGCTCGGC GGAGTCGTCC 
ACCTCGCAGA AGTCGCACAA GGGCAGCAGT CCCACGCCCA CGCCCACCGC CTCGCCCGGC 
CTCACTCCTT CGCCGTCGCC ACCCACAAGT GCGGGCGGTG AGCTGAGCAG CACGTCCGAG 
GGCGGAGCGG TACCGACAAT GGCGGCAGCA GCGTTCGCAG CGGACCACAG CGCTCTGGCA 
TCGGGGCCAA CGCTTCCGCA GACTCATCCC ACCTTCCATC CGCTGTTGCT GGAGGAGATC 
TACCGCTCGG GGTTTCCTTT TTTATGCCAA CCGGGCGGAC GGCGCGGCAT TGAGGCGCTG 
CTGGCGGGAG CCGCCAGTGC AGCTGCCCCA AAGCGGCCGC TTCCGCCCGT GAAGTTCTCA 
GCGGGCAGTG TGGTGGGACT CAAGACGAAG GATCGCTACA GCTGCAAGTT CTGCGGCAAG 
GTGTTCCCCA GATCGGCCAA TCTGACGAGG CATTTGAGGA CGCACACGGG CGAGCAGCCG 
TATCCCTGCA AGTACTGCGA TCGGGCATTC AGTATATCCT CGAACCTGCA GCGCCATGTG 
AGGAACATCC ACAACAAGGA GCGTCCTTTT CGCTGCGAGC TCTGCGATAG ATCCTTTGGC 
CAGCAGACGA ATCTCGATCG GCACGTAAAG AAGCACGAGT CGGAGGGCAA CAATTTCCGG 
GATTCGCCCA GTTCCAGTGG CATTGCCGAA AGGGAGGAGT ACTTCGATGA CATAAGGAAG 
TTCATGAACC GAGTCTACAC GCCCAACAGT CTGGCCGGCA ATGAGGGCGA TACCGAGGAG 
TATCCCAATA GCGACGATCA GTCCGTCAAT CTGGAAAAGG ACAGTGGCAA CTTCAACAAC 
AACAGCAGCA ACATCAGCAA CAACAACAGC AGCAGCGGCA ACAACAACAA CAGCTCCAAG 
GCCATTACAA TAAGCTCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ham ( NM_001273615.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260544.1 Drosophila melanogaster hamlet (ham), transcript variant E, mRNA.

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CloneID	OFa16008
Clone ID Related Accession (Same CDS sequence)	NM_001273615.1
Accession Version	NM_001273615.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	hamlet, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCACGCCA CCTGTCGGAT GCAGATCAAG TGCGACAAGT GCAACCAGAG CTTCAGCACG 
TTGACCTCCC TGACCAAGCA CAAAAAGTTC TGTGATTCAA CGGGTCCGGG TCCGTATCGA 
AACCAGCACG TGAATCGCCA CCATCAGCAT CCGCACCAGC ACCCACTTCC GCATCAGCCC 
CACCTGGCCG CCACTGCCAC ATCCACCTGT CCAGCGCCAC CAAGAGAATC TTCGGAGTCC 
TCGTCCTCAG CAGCAGCTGC CGCCGTTGCC GCCATGTCGA CGCCACCGAA TCCTTTCCTA 
ATGTTCCGCA CTGCGCCGTC TTTCTTTCCC GGATTTCCGC CTTACGGATT TCCCCCCTTT 
CTACCACAAA ATCCGCTGCA TCCTACGAAC ATTCCGATGT TTTTCTCGAA AAATCCCATG 
GATTTGGGTT GCGGTGGTCC GGAGATCACT TCTCCCGTTT CCGCCTTTGA TCAGAAACTA 
CCATTTGGCT TTCTAAAGGG TGAGAACAGT GAATCGCAGG CCTATGACAA AGTTACGGAG 
AAGGAGTTGG TCTTCAAGGC GGAAGAAAAA CTGAAGAAAG AGCCACTTGT GCAGGCATTC 
GAAGGCGAAG AGGACGAGAG CCGGAGTTCC CTGGACATTA AGGGTAAATT GGAGGACACC 
AGAAACGACT CCAAATCTGA GGAGCAGGAC GATATGAAGC AGGAACCCGA AAGGGTTAGC 
ACTCCCGATC AACAGCAGGC CGAGGATGAT AGGAAATCCA TTGACATCAT GAGCACACCA 
CCGCCCGCAG ACACACCCTC TGGAGGAGAT GGACCGTTGG ACCTTTCCAT CTGCCGCAAA 
CGCTCGGCTG GATCATTCTT TACCGCACCG GCAGAGGACA ACCTTATGCT CCATAGGTTT 
ATGCCCAGAT TACACGAATT CGAAGCCGAA AGGGGGCAGC CGCTGAAAAT GCGCAAATCA 
CACAGCTCGG CGGAGTCGTC CACCTCGCAG AAGTCGCACA AGGGCAGCAG TCCCACGCCC 
ACGCCCACCG CCTCGCCCGG CCTCACTCCT TCGCCGTCGC CACCCACAAG TGCGGGCGGT 
GAGCTGAGCA GCACGTCCGA GGGCGGAGCG GTACCGACAA TGGCGGCAGC AGCGTTCGCA 
GCGGACCACA GCGCTCTGGC ATCGGGGCCA ACGCTTCCGC AGACTCATCC CACCTTCCAT 
CCGCTGTTGC TGGAGGAGAT CTACCGCTCG GGGTTTCCTT TTTTATGCCA ACCGGGCGGA 
CGGCGCGGCA TTGAGGCGCT GCTGGCGGGA GCCGCCAGTG CAGCTGCCCC AAAGCGGCCG 
CTTCCGCCCG TGAAGTTCTC AGCGGGCAGT GTGGTGGGAC TCAAGACGAA GGATCGCTAC 
AGCTGCAAGT TCTGCGGCAA GGTGTTCCCC AGATCGGCCA ATCTGACGAG GCATTTGAGG 
ACGCACACGG GCGAGCAGCC GTATCCCTGC AAGTACTGCG ATCGGGCATT CAGTATATCC 
TCGAACCTGC AGCGCCATGT GAGGAACATC CACAACAAGG AGCGTCCTTT TCGCTGCGAG 
CTCTGCGATA GATCCTTTGG CCAGCAGACG AATCTCGATC GGCACGTAAA GAAGCACGAG 
TCGGAGGGCA ACAATTTCCG GGATTCGCCC AGTTCCAGTG GCATTGCCGA AAGGGAGGAG 
TACTTCGATG ACATAAGGAA GTTCATGAAC CGAGTCTACA CGCCCAACAG TCTGGCCGGC 
AATGAGGGCG ATACCGAGGA GTATCCCAAT AGCGACGATC AGTCCGTCAA TCTGGAAAAG 
GACAGTGGCA ACTTCAACAA CAACAGCAGC AACATCAGCA ACAACAACAG CAGCAGCGGC 
AACAACAACA ACAGCTCCAA GGCCATTACA ATAAGCTCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260544.1
CDS	686..2587
Translation	MHATCRMQIKCDKCNQSFSTLTSLTKHKKFCDSTGPGPYRNQHVNRHHQHPHQHPLPHQPHLAATATSTCPAPPRESSESSSSAAAAAVAAMSTPPNPFLMFRTAPSFFPGFPPYGFPPFLPQNPLHPTNIPMFFSKNPMDLGCGGPEITSPVSAFDQKLPFGFLKGENSESQAYDKVTEKELVFKAEEKLKKEPLVQAFEGEEDESRSSLDIKGKLEDTRNDSKSEEQDDMKQEPERVSTPDQQQAEDDRKSIDIMSTPPPADTPSGGDGPLDLSICRKRSAGSFFTAPAEDNLMLHRFMPRLHEFEAERGQPLKMRKSHSSAESSTSQKSHKGSSPTPTPTASPGLTPSPSPPTSAGGELSSTSEGGAVPTMAAAAFAADHSALASGPTLPQTHPTFHPLLLEEIYRSGFPFLCQPGGRRGIEALLAGAASAAAPKRPLPPVKFSAGSVVGLKTKDRYSCKFCGKVFPRSANLTRHLRTHTGEQPYPCKYCDRAFSISSNLQRHVRNIHNKERPFRCELCDRSFGQQTNLDRHVKKHESEGNNFRDSPSSSGIAEREEYFDDIRKFMNRVYTPNSLAGNEGDTEEYPNSDDQSVNLEKDSGNFNNNSSNISNNNSSSGNNNNSSKAITISS

Target ORF information:

RefSeq Version	NM_001273615.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster hamlet (ham), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273615.1

ORF Insert Sequence:

ATGCACGCCA CCTGTCGGAT GCAGATCAAG TGCGACAAGT GCAACCAGAG CTTCAGCACG 
TTGACCTCCC TGACCAAGCA CAAAAAGTTC TGTGATTCAA CGGGTCCGGG TCCGTATCGA 
AACCAGCACG TGAATCGCCA CCATCAGCAT CCGCACCAGC ACCCACTTCC GCATCAGCCC 
CACCTGGCCG CCACTGCCAC ATCCACCTGT CCAGCGCCAC CAAGAGAATC TTCGGAGTCC 
TCGTCCTCAG CAGCAGCTGC CGCCGTTGCC GCCATGTCGA CGCCACCGAA TCCTTTCCTA 
ATGTTCCGCA CTGCGCCGTC TTTCTTTCCC GGATTTCCGC CTTACGGATT TCCCCCCTTT 
CTACCACAAA ATCCGCTGCA TCCTACGAAC ATTCCGATGT TTTTCTCGAA AAATCCCATG 
GATTTGGGTT GCGGTGGTCC GGAGATCACT TCTCCCGTTT CCGCCTTTGA TCAGAAACTA 
CCATTTGGCT TTCTAAAGGG TGAGAACAGT GAATCGCAGG CCTATGACAA AGTTACGGAG 
AAGGAGTTGG TCTTCAAGGC GGAAGAAAAA CTGAAGAAAG AGCCACTTGT GCAGGCATTC 
GAAGGCGAAG AGGACGAGAG CCGGAGTTCC CTGGACATTA AGGGTAAATT GGAGGACACC 
AGAAACGACT CCAAATCTGA GGAGCAGGAC GATATGAAGC AGGAACCCGA AAGGGTTAGC 
ACTCCCGATC AACAGCAGGC CGAGGATGAT AGGAAATCCA TTGACATCAT GAGCACACCA 
CCGCCCGCAG ACACACCCTC TGGAGGAGAT GGACCGTTGG ACCTTTCCAT CTGCCGCAAA 
CGCTCGGCTG GATCATTCTT TACCGCACCG GCAGAGGACA ACCTTATGCT CCATAGGTTT 
ATGCCCAGAT TACACGAATT CGAAGCCGAA AGGGGGCAGC CGCTGAAAAT GCGCAAATCA 
CACAGCTCGG CGGAGTCGTC CACCTCGCAG AAGTCGCACA AGGGCAGCAG TCCCACGCCC 
ACGCCCACCG CCTCGCCCGG CCTCACTCCT TCGCCGTCGC CACCCACAAG TGCGGGCGGT 
GAGCTGAGCA GCACGTCCGA GGGCGGAGCG GTACCGACAA TGGCGGCAGC AGCGTTCGCA 
GCGGACCACA GCGCTCTGGC ATCGGGGCCA ACGCTTCCGC AGACTCATCC CACCTTCCAT 
CCGCTGTTGC TGGAGGAGAT CTACCGCTCG GGGTTTCCTT TTTTATGCCA ACCGGGCGGA 
CGGCGCGGCA TTGAGGCGCT GCTGGCGGGA GCCGCCAGTG CAGCTGCCCC AAAGCGGCCG 
CTTCCGCCCG TGAAGTTCTC AGCGGGCAGT GTGGTGGGAC TCAAGACGAA GGATCGCTAC 
AGCTGCAAGT TCTGCGGCAA GGTGTTCCCC AGATCGGCCA ATCTGACGAG GCATTTGAGG 
ACGCACACGG GCGAGCAGCC GTATCCCTGC AAGTACTGCG ATCGGGCATT CAGTATATCC 
TCGAACCTGC AGCGCCATGT GAGGAACATC CACAACAAGG AGCGTCCTTT TCGCTGCGAG 
CTCTGCGATA GATCCTTTGG CCAGCAGACG AATCTCGATC GGCACGTAAA GAAGCACGAG 
TCGGAGGGCA ACAATTTCCG GGATTCGCCC AGTTCCAGTG GCATTGCCGA AAGGGAGGAG 
TACTTCGATG ACATAAGGAA GTTCATGAAC CGAGTCTACA CGCCCAACAG TCTGGCCGGC 
AATGAGGGCG ATACCGAGGA GTATCCCAAT AGCGACGATC AGTCCGTCAA TCTGGAAAAG 
GACAGTGGCA ACTTCAACAA CAACAGCAGC AACATCAGCA ACAACAACAG CAGCAGCGGC 
AACAACAACA ACAGCTCCAA GGCCATTACA ATAAGCTCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ham cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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