Lrch cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lrch
Entrez Gene ID	35041
Full Name	Leucine-rich-repeats and calponin homology domain protein
Synonyms	CG6860,CT21229,Dmel\CG6860,dLRCH
General protein information	Preferred Names Leucine-rich-repeats and calponin homology domain protein Names CG6860 gene product from transcript CG6860-RA CG6860-PA CG6860-PB CG6860-PC Lrch-PA Lrch-PB Lrch-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 36C7-36C7

mRNA and Protein(s)

mRNA	Protein	Name
NM_165212.2	NP_724046.1	Leucine-rich-repeats and calponin homology domain protein, isoform A
NM_135990.3	NP_609834.2	Leucine-rich-repeats and calponin homology domain protein, isoform B
NM_001299083.1	NP_001286012.1	Leucine-rich-repeats and calponin homology domain protein, isoform C

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010012	XP_319156.4
Drosophila melanogaster (fruit fly)	Lrch	NP_724046.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Lrch gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lrch cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa14809	NM_165212.2 Latest version!	Drosophila melanogaster Leucine-rich-repeats and calponin homology domain protein (Lrch), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa14810	NM_135990.3 Latest version!	Drosophila melanogaster Leucine-rich-repeats and calponin homology domain protein (Lrch), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa21667	NM_001299083.1 Latest version!	Drosophila melanogaster Leucine-rich-repeats and calponin homology domain protein (Lrch), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lrch ( NM_165212.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724046.1 Drosophila melanogaster Leucine-rich-repeats and calponin homology domain protein (Lrch), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa14809
Clone ID Related Accession (Same CDS sequence)	NM_165212.2
Accession Version	NM_165212.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2430bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Leucine-rich-repeats and calponin homology domain protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165212.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAGCAA CGGTTTACCA ACGCCTACAT AGCGTCGCAG TCGGCGTCGG CAGCGCCGCT 
GGCAGCGCCA TCGGCAGCGG GTCGGCTTCC CTTCCAGGAT CTGGAACAGG ATCGGGATCT 
GGAATAGGCC ACGCTGGAAA CACCTCCTCC TCCACCTCGT CTGCCCTGCT TAGTCATTCC 
CAGCTGACGC GCTCCCTGGA AAGAATCCTG GAAGAAGCGC ACTTCAGCGG CGAACTGATC 
CTGACCAATC GCAAGCTGAA GGACTTTCCC AGGACTGGAA CGAAGTACTC ACTTACCGAC 
ACTGTCATCG CAGATCTTTC CCGGAATAGA TTTGCCGAGC TGCCCGAGGA AGTGACTACG 
TTTGCCTTCC TAGAGACCCT GCTACTGTAT CACAATACGA TTCGCAGCAT TCCGGAATCG 
GTGAAGCAGC TATCATCGTT GACCTATTTA GATTTGCGCA GCAATCAGTT GTCCTCGCTG 
CCACGCGAGA TTTGTTTCCT GCCTCTTCAG GTTCTGCTGG TTTCCAACAA TCGATTGACT 
TCGTTACCAG ATGAACTGGG TCGATTGGAT CAGACACTGA CCGATCTGGA TGCTTCGTAT 
AATCAGTTGG CCAATCTACC CGCTCGTCTG GGTGAGCTAA GATCCCTGCG CTCGCTCTCG 
TTGAGAAGCA ATCACTTGCT GTATCTGCCC AGAGAGCTTA CCTGTTTGAG TTTGATATCA 
CTGGACGTGA GCAACAACAA GATCGCTAGC CTGCCTCTCG AGATTCGTCA CATGAGCACT 
TTGGTAGAGC TTCAGCTAGA GAACAATCCT CTGACCTCGC CTCCAGCAAG CCTATGCATG 
CGCGGCCTGG TGCACGTGTT CAAGTTTTTG GAAACGCAGG CCACCAAGGA CGAGAAGGGA 
TCCCGGTCAG GTGGCAACTA CGATGGGTAC ACCACATTGC GAAGAGCTCC TCGACATAAT 
TCCAGCGGCA ATGTTCTTGA AGCTAGCACC ACTGGCAGCA CTAACAACCA GCGGAGGCAT 
CAAGTCGACT CGGGTTACAA CACCAGCGAT GGTTTGGACA AGCGCTGGTC GCACGATGCT 
CCTACTAAAT CGAAAACGGA TTCACCGCTG CATTGTGTTT CCAATTCGAC AGCAGCCACT 
TTGCCCAGGA CGCTGCCAAG TGCGGTTCAT TCGCAAGCGG ATCTAATGGA CGCATCCCTT 
ACCTCCAGCA CTAGCACCAT TGTGGATGAG AGTCTCACCT TGAGTCCCAC AGCATCGCCC 
TGCAAGTTAA GCTATGCACA CTCGAATAGC TCCAGTAATG GATCGTCGCC AGATCAGGAC 
GATGACATAA TGCTGGACCA CCAGGAGCGC ACTGTTCACC ACGCCAATGG AAGATCCGGA 
AAAGGTCTTG GCAATATTCA GACCTACAAG GAGTACAAGG AGGCCCTTAA GCAGCAGCGC 
AACCAGGAGA TCAGCGTCTA TAAGCAAAAG CATCCGAATG CCAACCACAG TCCCAATGAC 
GACGAGGATC TTTCTCCGTC ACCGCCGAGC ATTTCAAACG GATCTTCCTC GAACACACTG 
CCAAAGTCTA TTATGAAACA GAATAGCAAC CAAATTGGTC ACAATGGGAA TGGAAATGGA 
AGCGCGAACG GAAATGGAGT CGATGATGTT GTTATACCAA AGAAACCCAT CCAGAAGGTG 
ATTCCCTCTC GGATTACAGA AATAAAGCCA GCATCCACAT CCGACATTCG CTCCGCAAAT 
TCATCGGATT GTTTGGGCTA CGTTAAGCCA CAGAGTCCCA TGAAGAATGG CACAAGCAAA 
TTTAATACGT CCAATGGTGG AGGAGTCCAA ACAACAGTGG GAATAGTCAG TAGCACCACC 
ATTAAATCCC CAGTCAGCTC AACCACCAAA CTTGGCACAG TAAAGACGCA TCGTTCGGTG 
ACCTGGAACC ATGACATCCC CGCGGAGAAG CTGAGCTTCA CCATGCGCCG GGAGTTTGAT 
CGCCAAAGGG AGGAGACTGA GTTGATGTCA CAGCTGAGAA CTATCATTGA GACGCGTCTC 
AAGATGACGT TGCCTGTGGA TATTGCCTCT GCTCTGACGG ATGGGGTTAT ACTGTGTCAT 
TTGGCCAACT ATGTGCGTCC GCGATCAGTG GCCAGCATTC ATGTGCCATC TCCCGGTGTC 
AATAAGTTGA CAATGGCCAG GTGCCGGCGG AATGTGGATA ACTTTCTGGA GGCGTGTCGA 
CGCATTGGCG TAGATGAGAA TCTAATTTGC TGCGCTGCCG ACGTGCTGGA GGGTAAAGGT 
GCCGTCCAAG TGGCCATTAC GGTGGGTGAG CTTTTCCGGC TTCATGGAAA TGGTGGCTGC 
GGCAGTGGCA ACAGCAGTGG AGCAGCCACG CCCACCAAGT CGCCTACCAG AACGACACGT 
GCCACAATGT CGCCAACGCC ATTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724046.1
CDS	334..2763
Translation	MAATVYQRLHSVAVGVGSAAGSAIGSGSASLPGSGTGSGSGIGHAGNTSSSTSSALLSHSQLTRSLERILEEAHFSGELILTNRKLKDFPRTGTKYSLTDTVIADLSRNRFAELPEEVTTFAFLETLLLYHNTIRSIPESVKQLSSLTYLDLRSNQLSSLPREICFLPLQVLLVSNNRLTSLPDELGRLDQTLTDLDASYNQLANLPARLGELRSLRSLSLRSNHLLYLPRELTCLSLISLDVSNNKIASLPLEIRHMSTLVELQLENNPLTSPPASLCMRGLVHVFKFLETQATKDEKGSRSGGNYDGYTTLRRAPRHNSSGNVLEASTTGSTNNQRRHQVDSGYNTSDGLDKRWSHDAPTKSKTDSPLHCVSNSTAATLPRTLPSAVHSQADLMDASLTSSTSTIVDESLTLSPTASPCKLSYAHSNSSSNGSSPDQDDDIMLDHQERTVHHANGRSGKGLGNIQTYKEYKEALKQQRNQEISVYKQKHPNANHSPNDDEDLSPSPPSISNGSSSNTLPKSIMKQNSNQIGHNGNGNGSANGNGVDDVVIPKKPIQKVIPSRITEIKPASTSDIRSANSSDCLGYVKPQSPMKNGTSKFNTSNGGGVQTTVGIVSSTTIKSPVSSTTKLGTVKTHRSVTWNHDIPAEKLSFTMRREFDRQREETELMSQLRTIIETRLKMTLPVDIASALTDGVILCHLANYVRPRSVASIHVPSPGVNKLTMARCRRNVDNFLEACRRIGVDENLICCAADVLEGKGAVQVAITVGELFRLHGNGGCGSGNSSGAATPTKSPTRTTRATMSPTPLA

Target ORF information:

RefSeq Version	NM_165212.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Leucine-rich-repeats and calponin homology domain protein (Lrch), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165212.2

ORF Insert Sequence:

ATGGCAGCAA CGGTTTACCA ACGCCTACAT AGCGTCGCAG TCGGCGTCGG CAGCGCCGCT 
GGCAGCGCCA TCGGCAGCGG GTCGGCTTCC CTTCCAGGAT CTGGAACAGG ATCGGGATCT 
GGAATAGGCC ACGCTGGAAA CACCTCCTCC TCCACCTCGT CTGCCCTGCT TAGTCATTCC 
CAGCTGACGC GCTCCCTGGA AAGAATCCTG GAAGAAGCGC ACTTCAGCGG CGAACTGATC 
CTGACCAATC GCAAGCTGAA GGACTTTCCC AGGACTGGAA CGAAGTACTC ACTTACCGAC 
ACTGTCATCG CAGATCTTTC CCGGAATAGA TTTGCCGAGC TGCCCGAGGA AGTGACTACG 
TTTGCCTTCC TAGAGACCCT GCTACTGTAT CACAATACGA TTCGCAGCAT TCCGGAATCG 
GTGAAGCAGC TATCATCGTT GACCTATTTA GATTTGCGCA GCAATCAGTT GTCCTCGCTG 
CCACGCGAGA TTTGTTTCCT GCCTCTTCAG GTTCTGCTGG TTTCCAACAA TCGATTGACT 
TCGTTACCAG ATGAACTGGG TCGATTGGAT CAGACACTGA CCGATCTGGA TGCTTCGTAT 
AATCAGTTGG CCAATCTACC CGCTCGTCTG GGTGAGCTAA GATCCCTGCG CTCGCTCTCG 
TTGAGAAGCA ATCACTTGCT GTATCTGCCC AGAGAGCTTA CCTGTTTGAG TTTGATATCA 
CTGGACGTGA GCAACAACAA GATCGCTAGC CTGCCTCTCG AGATTCGTCA CATGAGCACT 
TTGGTAGAGC TTCAGCTAGA GAACAATCCT CTGACCTCGC CTCCAGCAAG CCTATGCATG 
CGCGGCCTGG TGCACGTGTT CAAGTTTTTG GAAACGCAGG CCACCAAGGA CGAGAAGGGA 
TCCCGGTCAG GTGGCAACTA CGATGGGTAC ACCACATTGC GAAGAGCTCC TCGACATAAT 
TCCAGCGGCA ATGTTCTTGA AGCTAGCACC ACTGGCAGCA CTAACAACCA GCGGAGGCAT 
CAAGTCGACT CGGGTTACAA CACCAGCGAT GGTTTGGACA AGCGCTGGTC GCACGATGCT 
CCTACTAAAT CGAAAACGGA TTCACCGCTG CATTGTGTTT CCAATTCGAC AGCAGCCACT 
TTGCCCAGGA CGCTGCCAAG TGCGGTTCAT TCGCAAGCGG ATCTAATGGA CGCATCCCTT 
ACCTCCAGCA CTAGCACCAT TGTGGATGAG AGTCTCACCT TGAGTCCCAC AGCATCGCCC 
TGCAAGTTAA GCTATGCACA CTCGAATAGC TCCAGTAATG GATCGTCGCC AGATCAGGAC 
GATGACATAA TGCTGGACCA CCAGGAGCGC ACTGTTCACC ACGCCAATGG AAGATCCGGA 
AAAGGTCTTG GCAATATTCA GACCTACAAG GAGTACAAGG AGGCCCTTAA GCAGCAGCGC 
AACCAGGAGA TCAGCGTCTA TAAGCAAAAG CATCCGAATG CCAACCACAG TCCCAATGAC 
GACGAGGATC TTTCTCCGTC ACCGCCGAGC ATTTCAAACG GATCTTCCTC GAACACACTG 
CCAAAGTCTA TTATGAAACA GAATAGCAAC CAAATTGGTC ACAATGGGAA TGGAAATGGA 
AGCGCGAACG GAAATGGAGT CGATGATGTT GTTATACCAA AGAAACCCAT CCAGAAGGTG 
ATTCCCTCTC GGATTACAGA AATAAAGCCA GCATCCACAT CCGACATTCG CTCCGCAAAT 
TCATCGGATT GTTTGGGCTA CGTTAAGCCA CAGAGTCCCA TGAAGAATGG CACAAGCAAA 
TTTAATACGT CCAATGGTGG AGGAGTCCAA ACAACAGTGG GAATAGTCAG TAGCACCACC 
ATTAAATCCC CAGTCAGCTC AACCACCAAA CTTGGCACAG TAAAGACGCA TCGTTCGGTG 
ACCTGGAACC ATGACATCCC CGCGGAGAAG CTGAGCTTCA CCATGCGCCG GGAGTTTGAT 
CGCCAAAGGG AGGAGACTGA GTTGATGTCA CAGCTGAGAA CTATCATTGA GACGCGTCTC 
AAGATGACGT TGCCTGTGGA TATTGCCTCT GCTCTGACGG ATGGGGTTAT ACTGTGTCAT 
TTGGCCAACT ATGTGCGTCC GCGATCAGTG GCCAGCATTC ATGTGCCATC TCCCGGTGTC 
AATAAGTTGA CAATGGCCAG GTGCCGGCGG AATGTGGATA ACTTTCTGGA GGCGTGTCGA 
CGCATTGGCG TAGATGAGAA TCTAATTTGC TGCGCTGCCG ACGTGCTGGA GGGTAAAGGT 
GCCGTCCAAG TGGCCATTAC GGTGGGTGAG CTTTTCCGGC TTCATGGAAA TGGTGGCTGC 
GGCAGTGGCA ACAGCAGTGG AGCAGCCACG CCCACCAAGT CGCCTACCAG AACGACACGT 
GCCACAATGT CGCCAACGCC ATTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lrch ( NM_135990.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609834.2 Drosophila melanogaster Leucine-rich-repeats and calponin homology domain protein (Lrch), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa14810
Clone ID Related Accession (Same CDS sequence)	NM_135990.3
Accession Version	NM_135990.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Leucine-rich-repeats and calponin homology domain protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_135990.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAGCAA CGGTTTACCA ACGCCTACAT AGCGTCGCAG TCGGCGTCGG CAGCGCCGCT 
GGCAGCGCCA TCGGCAGCGG GTCGGCTTCC CTTCCAGGAT CTGGAACAGG ATCGGGATCT 
GGAATAGGCC ACGCTGGAAA CACCTCCTCC TCCACCTCGT CTGCCCTGCT TAGTCATTCC 
CAGCTGACGC GCTCCCTGGA AAGAATCCTG GAAGAAGCGC ACTTCAGCGG CGAACTGATC 
CTGACCAATC GCAAGCTGAA GGACTTTCCC AGGACTGGAA CGAAGTACTC ACTTACCGAC 
ACTGTCATCG CAGATCTTTC CCGGAATAGA TTTGCCGAGC TGCCCGAGGA AGTGACTACG 
TTTGCCTTCC TAGAGACCCT GCTACTGTAT CACAATACGA TTCGCAGCAT TCCGGAATCG 
GTGAAGCAGC TATCATCGTT GACCTATTTA GATTTGCGCA GCAATCAGTT GTCCTCGCTG 
CCACGCGAGA TTTGTTTCCT GCCTCTTCAG GTTCTGCTGG TTTCCAACAA TCGATTGACT 
TCGTTACCAG ATGAACTGGG TCGATTGGAT CAGACACTGA CCGATCTGGA TGCTTCGTAT 
AATCAGTTGG CCAATCTACC CGCTCGTCTG GGTGAGCTAA GATCCCTGCG CTCGCTCTCG 
TTGAGAAGCA ATCACTTGCT GTATCTGCCC AGAGAGCTTA CCTGTTTGAG TTTGATATCA 
CTGGACGTGA GCAACAACAA GATCGCTAGC CTGCCTCTCG AGATTCGTCA CATGAGCACT 
TTGGTAGAGC TTCAGCTAGA GAACAATCCT CTGACCTCGC CTCCAGCAAG CCTATGCATG 
CGCGGCCTGG TGCACGTGTT CAAGTTTTTG GAAACGCAGG CCACCAAGGA CGAGAAGGGA 
TCCCGGTCAG GTGGCAACTA CGATGGGTAC ACCACATTGC GAAGAGCTCC TCGACATAAT 
TCCAGCGGCA ATGTTCTTGA AGCTAGCACC ACTGGCAGCA CTAACAACCA GCGGAGGCAT 
CAAGTCGACT CGGGTTACAA CACCAGCGAT GGTTTGGACA AGCGCTGGTC GCACGATGCT 
CCTACTAAAT CGAAAACGGA TTCACCGCTG CATTGTGTTT CCAATTCGAC AGCAGCCACT 
TTGCCCAGGA CGCTGCCAAG TGCGGTTCAT TCGCAAGCGG ATCTAATGGA CGCATCCCTT 
ACCTCCAGCA CTAGCACCAT TGTGGATGAG AGTCTCACCT TGAGTCCCAC AGCATCGCCC 
TGCAAGTTAA GCTATGCACA CTCGAATAGC TCCAGTAATG GATCGTCGCC AGATCAGGAC 
GATGACATAA TGCTGGACCA CCAGGAGCGC ACTGTTCACC ACGCCAATGG AAGATCCGGA 
AAAGGTCTTG GCAATATTCA GACCTACAAG GAGTACAAGG AGGCCCTTAA GCAGCAGCGC 
AACCAGGAGA TCAGCGTCTA TAAGCAAAAG CATCCGAATG CCAACCACAG TCCCAATGAC 
GACGAGGATC TTTCTCCGTC ACCGCCGAGC ATTTCAAACG GATCTTCCTC GAACACACTG 
CCAAAGTCTA TTATGAAACA GAATAGCAAC CAAATTGGTC ACAATGGGAA TGGAAATGGA 
AGCGCGAACG GAAATGGAGT CGATGATGTT GTTATACCAA AGAAACCCAT CCAGAAGGTG 
ATTCCCTCTC GGATTACAGA AATAAAGCCA GCATCCACAT CCGACATTCG CTCCGCAAAT 
TCATCGGATT GTTTGGGCTA CGTTAAGCCA CAGAGTCCCA TGAAGAATGG CACAAGCAAA 
TTTAATACGT CCAATGGTGG AGGAGTCCAA ACAACAGTGG GAATAGTCAG TAGCACCACC 
ATTAAATCCC CAGTCAGCTC AACCACCAAA CTTGGCACAG TAAAGACGCA TCGTTCGGTG 
ACCTGGAACC ATGACATCCC CGCGGAGAAG CTGAGCTTCA CCATGCGCCG GGAGTTTGAT 
CGCCAAAGGG AGGAGACTGA GTTGATGTCA CAGCTGAGAA CTATCATTGA GACGCGTCTC 
AAGATGACGT TGCCTGTGGA TATTGCCTCT GCTCTGACGG ATGGGGTTAT ACTGTGTCAT 
TTGGCCAACT ATGTGCGTCC GCGATCAGTG GCCAGCATTC ATGTGCCATC TCCCGGTGTC 
AATAAGTTGA CAATGGCCAG GTGCCGGCGG AATGTGGATA ACTTTCTGGA GGCGTGTCGA 
CGCATTGGCG TAGATGAGGA GTTGATTTGC TCCTGCGAAG ATGTTGTGCC ACAAATTGAA 
CCGCAACAAC CACAATTGCC ACGTGCTGAC GATGACTATG ATGCTGATGT TTATGTCCAA 
GATGATGGCA GCATCAGCCG CAGCAATGAC AGCAGCCACC TGCAATGCAG CGACGATTGC 
AACAGCAGCA GCAACCATAT TAATAGAGTG CCCAATGCAT TGGCCCTACT GCGCACAGTG 
TCCGCTCTGA TTGCCATGGA TGAAAATCCG TATCATCAGC AGCTTCATCA TCATCTTCAG 
CTTCAGCTGG AGCAGCAACA CTTCGAGCAA GAGCAATTCA GTGAGCCAAC TACCTGCAGC 
CAACAGCAAG AACAGATGTT GCTGTTGCAG CAGCAACAGC AGCAGCTAGA GCACGTTTTG 
TATGAGAATG GCCTGCCGAG GGCTGAAAAT GATGATGGGG TTGAGGATCG TGATGATGAT 
ATAGGCAAGC GGCAGCAGCA ACTAGCGACA GTGGGTCAAA TGCTGCTGAA GGCGAAGCAA 
AAGACATATG AAAAAATAAA ACACAATCTG CTAAGTGCCC ATGGTCAGCA TAACTTTCAG 
GGCAATAATA ACAATAGAAT GTTGCATACG ATTGTGGAGA ATGAGCACGA TGTGGCCGCC 
GCAGGACCGG CGGGCCATTA TCAAATAATA GACGAAAAGC AAGAGGAGGA CCCTTACCAG 
CCAAAATCAG AGGACTTGAA GGATGCAGCC GCACTGGGCA ACGCGACCAA GGAAGCCATC 
AGCAAGCGGA TCGAGTTCTT CGAACAGCAG AAGAGTGGAA AACCAAAGCT GGAGGTCTTC 
AGCATCTATT TGCCGAAGGA GAAAACCAAG GAACTCGAGC TTGAGCAGAA AATAAAAGCA 
AAAACCAACG AAGCTGGATC TTCGATATTA AAGCACGATT CCCATACATT GACAGTGATC 
CTGTCATGCG TTTTCATCGC CACCTTCCTC TGGCTGAATC TAATTTGCTG CGCTGCCGAC 
GTGCTGGAGG GTAAAGGTGC CGTCCAAGTG GCCATTACGG TGGGTGAGCT TTTCCGGCTT 
CATGGAAATG GTGGCTGCGG CAGTGGCAAC AGCAGTGGAG CAGCCACGCC CACCAAGTCG 
CCTACCAGAA CGACACGTGC CACAATGTCG CCAACGCCAT TGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609834.2
CDS	334..3741
Translation	MAATVYQRLHSVAVGVGSAAGSAIGSGSASLPGSGTGSGSGIGHAGNTSSSTSSALLSHSQLTRSLERILEEAHFSGELILTNRKLKDFPRTGTKYSLTDTVIADLSRNRFAELPEEVTTFAFLETLLLYHNTIRSIPESVKQLSSLTYLDLRSNQLSSLPREICFLPLQVLLVSNNRLTSLPDELGRLDQTLTDLDASYNQLANLPARLGELRSLRSLSLRSNHLLYLPRELTCLSLISLDVSNNKIASLPLEIRHMSTLVELQLENNPLTSPPASLCMRGLVHVFKFLETQATKDEKGSRSGGNYDGYTTLRRAPRHNSSGNVLEASTTGSTNNQRRHQVDSGYNTSDGLDKRWSHDAPTKSKTDSPLHCVSNSTAATLPRTLPSAVHSQADLMDASLTSSTSTIVDESLTLSPTASPCKLSYAHSNSSSNGSSPDQDDDIMLDHQERTVHHANGRSGKGLGNIQTYKEYKEALKQQRNQEISVYKQKHPNANHSPNDDEDLSPSPPSISNGSSSNTLPKSIMKQNSNQIGHNGNGNGSANGNGVDDVVIPKKPIQKVIPSRITEIKPASTSDIRSANSSDCLGYVKPQSPMKNGTSKFNTSNGGGVQTTVGIVSSTTIKSPVSSTTKLGTVKTHRSVTWNHDIPAEKLSFTMRREFDRQREETELMSQLRTIIETRLKMTLPVDIASALTDGVILCHLANYVRPRSVASIHVPSPGVNKLTMARCRRNVDNFLEACRRIGVDEELICSCEDVVPQIEPQQPQLPRADDDYDADVYVQDDGSISRSNDSSHLQCSDDCNSSSNHINRVPNALALLRTVSALIAMDENPYHQQLHHHLQLQLEQQHFEQEQFSEPTTCSQQQEQMLLLQQQQQQLEHVLYENGLPRAENDDGVEDRDDDIGKRQQQLATVGQMLLKAKQKTYEKIKHNLLSAHGQHNFQGNNNNRMLHTIVENEHDVAAAGPAGHYQIIDEKQEEDPYQPKSEDLKDAAALGNATKEAISKRIEFFEQQKSGKPKLEVFSIYLPKEKTKELELEQKIKAKTNEAGSSILKHDSHTLTVILSCVFIATFLWLNLICCAADVLEGKGAVQVAITVGELFRLHGNGGCGSGNSSGAATPTKSPTRTTRATMSPTPLA

Target ORF information:

RefSeq Version	NM_135990.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Leucine-rich-repeats and calponin homology domain protein (Lrch), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135990.3

ORF Insert Sequence:

ATGGCAGCAA CGGTTTACCA ACGCCTACAT AGCGTCGCAG TCGGCGTCGG CAGCGCCGCT 
GGCAGCGCCA TCGGCAGCGG GTCGGCTTCC CTTCCAGGAT CTGGAACAGG ATCGGGATCT 
GGAATAGGCC ACGCTGGAAA CACCTCCTCC TCCACCTCGT CTGCCCTGCT TAGTCATTCC 
CAGCTGACGC GCTCCCTGGA AAGAATCCTG GAAGAAGCGC ACTTCAGCGG CGAACTGATC 
CTGACCAATC GCAAGCTGAA GGACTTTCCC AGGACTGGAA CGAAGTACTC ACTTACCGAC 
ACTGTCATCG CAGATCTTTC CCGGAATAGA TTTGCCGAGC TGCCCGAGGA AGTGACTACG 
TTTGCCTTCC TAGAGACCCT GCTACTGTAT CACAATACGA TTCGCAGCAT TCCGGAATCG 
GTGAAGCAGC TATCATCGTT GACCTATTTA GATTTGCGCA GCAATCAGTT GTCCTCGCTG 
CCACGCGAGA TTTGTTTCCT GCCTCTTCAG GTTCTGCTGG TTTCCAACAA TCGATTGACT 
TCGTTACCAG ATGAACTGGG TCGATTGGAT CAGACACTGA CCGATCTGGA TGCTTCGTAT 
AATCAGTTGG CCAATCTACC CGCTCGTCTG GGTGAGCTAA GATCCCTGCG CTCGCTCTCG 
TTGAGAAGCA ATCACTTGCT GTATCTGCCC AGAGAGCTTA CCTGTTTGAG TTTGATATCA 
CTGGACGTGA GCAACAACAA GATCGCTAGC CTGCCTCTCG AGATTCGTCA CATGAGCACT 
TTGGTAGAGC TTCAGCTAGA GAACAATCCT CTGACCTCGC CTCCAGCAAG CCTATGCATG 
CGCGGCCTGG TGCACGTGTT CAAGTTTTTG GAAACGCAGG CCACCAAGGA CGAGAAGGGA 
TCCCGGTCAG GTGGCAACTA CGATGGGTAC ACCACATTGC GAAGAGCTCC TCGACATAAT 
TCCAGCGGCA ATGTTCTTGA AGCTAGCACC ACTGGCAGCA CTAACAACCA GCGGAGGCAT 
CAAGTCGACT CGGGTTACAA CACCAGCGAT GGTTTGGACA AGCGCTGGTC GCACGATGCT 
CCTACTAAAT CGAAAACGGA TTCACCGCTG CATTGTGTTT CCAATTCGAC AGCAGCCACT 
TTGCCCAGGA CGCTGCCAAG TGCGGTTCAT TCGCAAGCGG ATCTAATGGA CGCATCCCTT 
ACCTCCAGCA CTAGCACCAT TGTGGATGAG AGTCTCACCT TGAGTCCCAC AGCATCGCCC 
TGCAAGTTAA GCTATGCACA CTCGAATAGC TCCAGTAATG GATCGTCGCC AGATCAGGAC 
GATGACATAA TGCTGGACCA CCAGGAGCGC ACTGTTCACC ACGCCAATGG AAGATCCGGA 
AAAGGTCTTG GCAATATTCA GACCTACAAG GAGTACAAGG AGGCCCTTAA GCAGCAGCGC 
AACCAGGAGA TCAGCGTCTA TAAGCAAAAG CATCCGAATG CCAACCACAG TCCCAATGAC 
GACGAGGATC TTTCTCCGTC ACCGCCGAGC ATTTCAAACG GATCTTCCTC GAACACACTG 
CCAAAGTCTA TTATGAAACA GAATAGCAAC CAAATTGGTC ACAATGGGAA TGGAAATGGA 
AGCGCGAACG GAAATGGAGT CGATGATGTT GTTATACCAA AGAAACCCAT CCAGAAGGTG 
ATTCCCTCTC GGATTACAGA AATAAAGCCA GCATCCACAT CCGACATTCG CTCCGCAAAT 
TCATCGGATT GTTTGGGCTA CGTTAAGCCA CAGAGTCCCA TGAAGAATGG CACAAGCAAA 
TTTAATACGT CCAATGGTGG AGGAGTCCAA ACAACAGTGG GAATAGTCAG TAGCACCACC 
ATTAAATCCC CAGTCAGCTC AACCACCAAA CTTGGCACAG TAAAGACGCA TCGTTCGGTG 
ACCTGGAACC ATGACATCCC CGCGGAGAAG CTGAGCTTCA CCATGCGCCG GGAGTTTGAT 
CGCCAAAGGG AGGAGACTGA GTTGATGTCA CAGCTGAGAA CTATCATTGA GACGCGTCTC 
AAGATGACGT TGCCTGTGGA TATTGCCTCT GCTCTGACGG ATGGGGTTAT ACTGTGTCAT 
TTGGCCAACT ATGTGCGTCC GCGATCAGTG GCCAGCATTC ATGTGCCATC TCCCGGTGTC 
AATAAGTTGA CAATGGCCAG GTGCCGGCGG AATGTGGATA ACTTTCTGGA GGCGTGTCGA 
CGCATTGGCG TAGATGAGGA GTTGATTTGC TCCTGCGAAG ATGTTGTGCC ACAAATTGAA 
CCGCAACAAC CACAATTGCC ACGTGCTGAC GATGACTATG ATGCTGATGT TTATGTCCAA 
GATGATGGCA GCATCAGCCG CAGCAATGAC AGCAGCCACC TGCAATGCAG CGACGATTGC 
AACAGCAGCA GCAACCATAT TAATAGAGTG CCCAATGCAT TGGCCCTACT GCGCACAGTG 
TCCGCTCTGA TTGCCATGGA TGAAAATCCG TATCATCAGC AGCTTCATCA TCATCTTCAG 
CTTCAGCTGG AGCAGCAACA CTTCGAGCAA GAGCAATTCA GTGAGCCAAC TACCTGCAGC 
CAACAGCAAG AACAGATGTT GCTGTTGCAG CAGCAACAGC AGCAGCTAGA GCACGTTTTG 
TATGAGAATG GCCTGCCGAG GGCTGAAAAT GATGATGGGG TTGAGGATCG TGATGATGAT 
ATAGGCAAGC GGCAGCAGCA ACTAGCGACA GTGGGTCAAA TGCTGCTGAA GGCGAAGCAA 
AAGACATATG AAAAAATAAA ACACAATCTG CTAAGTGCCC ATGGTCAGCA TAACTTTCAG 
GGCAATAATA ACAATAGAAT GTTGCATACG ATTGTGGAGA ATGAGCACGA TGTGGCCGCC 
GCAGGACCGG CGGGCCATTA TCAAATAATA GACGAAAAGC AAGAGGAGGA CCCTTACCAG 
CCAAAATCAG AGGACTTGAA GGATGCAGCC GCACTGGGCA ACGCGACCAA GGAAGCCATC 
AGCAAGCGGA TCGAGTTCTT CGAACAGCAG AAGAGTGGAA AACCAAAGCT GGAGGTCTTC 
AGCATCTATT TGCCGAAGGA GAAAACCAAG GAACTCGAGC TTGAGCAGAA AATAAAAGCA 
AAAACCAACG AAGCTGGATC TTCGATATTA AAGCACGATT CCCATACATT GACAGTGATC 
CTGTCATGCG TTTTCATCGC CACCTTCCTC TGGCTGAATC TAATTTGCTG CGCTGCCGAC 
GTGCTGGAGG GTAAAGGTGC CGTCCAAGTG GCCATTACGG TGGGTGAGCT TTTCCGGCTT 
CATGGAAATG GTGGCTGCGG CAGTGGCAAC AGCAGTGGAG CAGCCACGCC CACCAAGTCG 
CCTACCAGAA CGACACGTGC CACAATGTCG CCAACGCCAT TGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lrch ( NM_001299083.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286012.1 Drosophila melanogaster Leucine-rich-repeats and calponin homology domain protein (Lrch), transcript variant C, mRNA.

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CloneID	OFa21667
Clone ID Related Accession (Same CDS sequence)	NM_001299083.1
Accession Version	NM_001299083.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Leucine-rich-repeats and calponin homology domain protein, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAGCAA CGGTTTACCA ACGCCTACAT AGCGTCGCAG TCGGCGTCGG CAGCGCCGCT 
GGCAGCGCCA TCGGCAGCGG GTCGGCTTCC CTTCCAGGAT CTGGAACAGG ATCGGGATCT 
GGAATAGGCC ACGCTGGAAA CACCTCCTCC TCCACCTCGT CTGCCCTGCT TAGTCATTCC 
CAGCTGACGC GCTCCCTGGA AAGAATCCTG GAAGAAGCGC ACTTCAGCGG CGAACTGATC 
CTGACCAATC GCAAGCTGAA GGACTTTCCC AGGACTGGAA CGAAGTACTC ACTTACCGAC 
ACTGTCATCG CAGATCTTTC CCGGAATAGA TTTGCCGAGC TGCCCGAGGA AGTGACTACG 
TTTGCCTTCC TAGAGACCCT GCTACTGTAT CACAATACGA TTCGCAGCAT TCCGGAATCG 
GTGAAGCAGC TATCATCGTT GACCTATTTA GATTTGCGCA GCAATCAGTT GTCCTCGCTG 
CCACGCGAGA TTTGTTTCCT GCCTCTTCAG GTTCTGCTGG TTTCCAACAA TCGATTGACT 
TCGTTACCAG ATGAACTGGG TCGATTGGAT CAGACACTGA CCGATCTGGA TGCTTCGTAT 
AATCAGTTGG CCAATCTACC CGCTCGTCTG GGTGAGCTAA GATCCCTGCG CTCGCTCTCG 
TTGAGAAGCA ATCACTTGCT GTATCTGCCC AGAGAGCTTA CCTGTTTGAG TTTGATATCA 
CTGGACGTGA GCAACAACAA GATCGCTAGC CTGCCTCTCG AGATTCGTCA CATGAGCACT 
TTGGTAGAGC TTCAGCTAGA GAACAATCCT CTGACCTCGC CTCCAGCAAG CCTATGCATG 
CGCGGCCTGG TGCACGTGTT CAAGTTTTTG GAAACGCAGG CCACCAAGGA CGAGAAGGGA 
TCCCGGTCAG GTGGCAACTA CGATGGGTAC ACCACATTGC GAAGAGCTCC TCGACATAAT 
TCCAGCGGCA ATGTTCTTGA AGCTAGCACC ACTGGCAGCA CTAACAACCA GCGGAGGCAT 
CAAGTCGACT CGGGTTACAA CACCAGCGAT GGTTTGGACA AGCGCTGGTC GCACGATGCT 
CCTACTAAAT CGAAAACGGA TTCACCGCTG CATTGTGTTT CCAATTCGAC AGCAGCCACT 
TTGCCCAGGA CGCTGCCAAG TGCGGTTCAT TCGCAAGCGG ATCTAATGGA CGCATCCCTT 
ACCTCCAGCA CTAGCACCAT TGTGGATGAG AGTCTCACCT TGAGTCCCAC AGCATCGCCC 
TGCAAGTTAA GCTATGCACA CTCGAATAGC TCCAGTAATG GATCGTCGCC AGATCAGGAC 
GATGACATAA TGCTGGACCA CCAGGAGCGC ACTGTTCACC ACGCCAATGG AAGATCCGGA 
AAAGGTCTTG GCAATATTCA GACCTACAAG GAGTACAAGG AGGCCCTTAA GCAGCAGCGC 
AACCAGGAGA TCAGCGTCTA TAAGCAAAAG CATCCGAATG CCAACCACAG TCCCAATGAC 
GACGAGGATC TTTCTCCGTC ACCGCCGAGC ATTTCAAACG GATCTTCCTC GAACACACTG 
CCAAAGTCTA TTATGAAACA GAATAGCAAC CAAATTGGTC ACAATGGGAA TGGAAATGGA 
AGCGCGAACG GAAATGGAGT CGATGATGTT GTTATACCAA AGAAACCCAT CCAGAAGGTG 
ATTCCCTCTC GGATTACAGA AATAAAGCCA GCATCCACAT CCGACATTCG CTCCGCAAAT 
TCATCGGATT GTTTGGGCTA CGTTAAGCCA CAGAGTCCCA TGAAGAATGG CACAAGCAAA 
TTTAATACGT CCAATGGTGG AGGAGTCCAA ACAACAGTGG GAATAGTCAG TAGCACCACC 
ATTAAATCCC CAGTCAGCTC AACCACCAAA CTTGGCACAG TAAAGACGCA TCGTTCGGTG 
ACCTGGAACC ATGACATCCC CGCGGAGAAG CTGAGCTTCA CCATGCGCCG GGAGTTTGAT 
CGCCAAAGGG AGGAGACTGA GTTGATGTCA CAGCTGAGAA CTATCATTGA GACGCGTCTC 
AAGATGACGT TGCCTGTGGA TATTGCCTCT GCTCTGACGG ATGGGGTTAT ACTGTGTCAT 
TTGGCCAACT ATGTGCGTCC GCGATCAGTG GCCAGCATTC ATGTGCCATC TCCCGGTGTC 
AATAAGTTGA CAATGGCCAG GTGCCGGCGG AATGTGGATA ACTTTCTGGA GGCGTGTCGA 
CGCATTGGCG TAGATGAGGA GTTGATTTGC TCCTGCGAAG ATGTTGTGCC ACAAATTGAA 
CCGCAACAAC CACAATTGCC ACGTGCTGAC GATGACTATG ATGCTGATGT TTATGTCCAA 
GATGATGGCA GCATCAGCCG CAGCAATGAC AGCAGCCACC TGCAATGCAG CGACGATTGC 
AACAGCAGCA GCAACCATAT TAATAGAGTG CCCAATGCAT TGGCCCTACT GCGCACAGTG 
TCCGCTCTGA TTGCCATGGA TGAAAATCCG TATCATCAGC AGCTTCATCA TCATCTTCAG 
CTTCAGCTGG AGCAGCAACA CTTCGAGCAA GAGCAATTCA GTGAGCCAAC TACCTGCAGC 
CAACAGCAAG AACAGATGTT GCTGTTGCAG CAGCAACAGC AGCAGCTAGA GCACGTTTTG 
TATGAGAATG GCCTGCCGAG GGCTGAAAAT GATGATGGGG TTGAGGATCG TGATGATGAT 
ATAGGCAAGC GGCAGCAGCA ACTAGCGACA GTGGGTCAAA TGCTGCTGAA GGCGAAGCAA 
AAGACATATG AAAAAATAAA ACACAATCTG CTAAGTGCCC ATGGTCAGCA TAACTTTCAG 
GGCAATAATA ACAATAGAAT GTTGCATACG ATTGTGGAGA ATGAGCACGA TGTGGCCGCC 
GCAGGACCGG CGGGCCATTA TCAAATAATA GACGAAAAGC AAGAGGAGGA CCCTTACCAG 
CCAAAATCAG AGGACTTGAA GGATGCAGCC GCACTGGGCA ACGCGACCAA GGAAGCCATC 
AGCAAGCGGA TCGAGTTCTT CGAACAGCAG AAGAGTGGAA AACCAAAGCT GGAGGTCTTC 
AGCATCTATT TGCCGAAGGA GAAAACCAAG GAACTCGAGC TTGAGCAGAA AATAAAAGCA 
AAAACCAACG AAGCTGGATC TTCGATATTA AAGCACGATT CCCATACATT GACAGTGATC 
CTGTCATGCG TTTTCATCGC CACCTTCCTC TGGCTGGTAT TTTTCCCGCT GCCTGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286012.1
CDS	334..3573
Translation	MAATVYQRLHSVAVGVGSAAGSAIGSGSASLPGSGTGSGSGIGHAGNTSSSTSSALLSHSQLTRSLERILEEAHFSGELILTNRKLKDFPRTGTKYSLTDTVIADLSRNRFAELPEEVTTFAFLETLLLYHNTIRSIPESVKQLSSLTYLDLRSNQLSSLPREICFLPLQVLLVSNNRLTSLPDELGRLDQTLTDLDASYNQLANLPARLGELRSLRSLSLRSNHLLYLPRELTCLSLISLDVSNNKIASLPLEIRHMSTLVELQLENNPLTSPPASLCMRGLVHVFKFLETQATKDEKGSRSGGNYDGYTTLRRAPRHNSSGNVLEASTTGSTNNQRRHQVDSGYNTSDGLDKRWSHDAPTKSKTDSPLHCVSNSTAATLPRTLPSAVHSQADLMDASLTSSTSTIVDESLTLSPTASPCKLSYAHSNSSSNGSSPDQDDDIMLDHQERTVHHANGRSGKGLGNIQTYKEYKEALKQQRNQEISVYKQKHPNANHSPNDDEDLSPSPPSISNGSSSNTLPKSIMKQNSNQIGHNGNGNGSANGNGVDDVVIPKKPIQKVIPSRITEIKPASTSDIRSANSSDCLGYVKPQSPMKNGTSKFNTSNGGGVQTTVGIVSSTTIKSPVSSTTKLGTVKTHRSVTWNHDIPAEKLSFTMRREFDRQREETELMSQLRTIIETRLKMTLPVDIASALTDGVILCHLANYVRPRSVASIHVPSPGVNKLTMARCRRNVDNFLEACRRIGVDEELICSCEDVVPQIEPQQPQLPRADDDYDADVYVQDDGSISRSNDSSHLQCSDDCNSSSNHINRVPNALALLRTVSALIAMDENPYHQQLHHHLQLQLEQQHFEQEQFSEPTTCSQQQEQMLLLQQQQQQLEHVLYENGLPRAENDDGVEDRDDDIGKRQQQLATVGQMLLKAKQKTYEKIKHNLLSAHGQHNFQGNNNNRMLHTIVENEHDVAAAGPAGHYQIIDEKQEEDPYQPKSEDLKDAAALGNATKEAISKRIEFFEQQKSGKPKLEVFSIYLPKEKTKELELEQKIKAKTNEAGSSILKHDSHTLTVILSCVFIATFLWLVFFPLPG

Target ORF information:

RefSeq Version	NM_001299083.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Leucine-rich-repeats and calponin homology domain protein (Lrch), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299083.1

ORF Insert Sequence:

ATGGCAGCAA CGGTTTACCA ACGCCTACAT AGCGTCGCAG TCGGCGTCGG CAGCGCCGCT 
GGCAGCGCCA TCGGCAGCGG GTCGGCTTCC CTTCCAGGAT CTGGAACAGG ATCGGGATCT 
GGAATAGGCC ACGCTGGAAA CACCTCCTCC TCCACCTCGT CTGCCCTGCT TAGTCATTCC 
CAGCTGACGC GCTCCCTGGA AAGAATCCTG GAAGAAGCGC ACTTCAGCGG CGAACTGATC 
CTGACCAATC GCAAGCTGAA GGACTTTCCC AGGACTGGAA CGAAGTACTC ACTTACCGAC 
ACTGTCATCG CAGATCTTTC CCGGAATAGA TTTGCCGAGC TGCCCGAGGA AGTGACTACG 
TTTGCCTTCC TAGAGACCCT GCTACTGTAT CACAATACGA TTCGCAGCAT TCCGGAATCG 
GTGAAGCAGC TATCATCGTT GACCTATTTA GATTTGCGCA GCAATCAGTT GTCCTCGCTG 
CCACGCGAGA TTTGTTTCCT GCCTCTTCAG GTTCTGCTGG TTTCCAACAA TCGATTGACT 
TCGTTACCAG ATGAACTGGG TCGATTGGAT CAGACACTGA CCGATCTGGA TGCTTCGTAT 
AATCAGTTGG CCAATCTACC CGCTCGTCTG GGTGAGCTAA GATCCCTGCG CTCGCTCTCG 
TTGAGAAGCA ATCACTTGCT GTATCTGCCC AGAGAGCTTA CCTGTTTGAG TTTGATATCA 
CTGGACGTGA GCAACAACAA GATCGCTAGC CTGCCTCTCG AGATTCGTCA CATGAGCACT 
TTGGTAGAGC TTCAGCTAGA GAACAATCCT CTGACCTCGC CTCCAGCAAG CCTATGCATG 
CGCGGCCTGG TGCACGTGTT CAAGTTTTTG GAAACGCAGG CCACCAAGGA CGAGAAGGGA 
TCCCGGTCAG GTGGCAACTA CGATGGGTAC ACCACATTGC GAAGAGCTCC TCGACATAAT 
TCCAGCGGCA ATGTTCTTGA AGCTAGCACC ACTGGCAGCA CTAACAACCA GCGGAGGCAT 
CAAGTCGACT CGGGTTACAA CACCAGCGAT GGTTTGGACA AGCGCTGGTC GCACGATGCT 
CCTACTAAAT CGAAAACGGA TTCACCGCTG CATTGTGTTT CCAATTCGAC AGCAGCCACT 
TTGCCCAGGA CGCTGCCAAG TGCGGTTCAT TCGCAAGCGG ATCTAATGGA CGCATCCCTT 
ACCTCCAGCA CTAGCACCAT TGTGGATGAG AGTCTCACCT TGAGTCCCAC AGCATCGCCC 
TGCAAGTTAA GCTATGCACA CTCGAATAGC TCCAGTAATG GATCGTCGCC AGATCAGGAC 
GATGACATAA TGCTGGACCA CCAGGAGCGC ACTGTTCACC ACGCCAATGG AAGATCCGGA 
AAAGGTCTTG GCAATATTCA GACCTACAAG GAGTACAAGG AGGCCCTTAA GCAGCAGCGC 
AACCAGGAGA TCAGCGTCTA TAAGCAAAAG CATCCGAATG CCAACCACAG TCCCAATGAC 
GACGAGGATC TTTCTCCGTC ACCGCCGAGC ATTTCAAACG GATCTTCCTC GAACACACTG 
CCAAAGTCTA TTATGAAACA GAATAGCAAC CAAATTGGTC ACAATGGGAA TGGAAATGGA 
AGCGCGAACG GAAATGGAGT CGATGATGTT GTTATACCAA AGAAACCCAT CCAGAAGGTG 
ATTCCCTCTC GGATTACAGA AATAAAGCCA GCATCCACAT CCGACATTCG CTCCGCAAAT 
TCATCGGATT GTTTGGGCTA CGTTAAGCCA CAGAGTCCCA TGAAGAATGG CACAAGCAAA 
TTTAATACGT CCAATGGTGG AGGAGTCCAA ACAACAGTGG GAATAGTCAG TAGCACCACC 
ATTAAATCCC CAGTCAGCTC AACCACCAAA CTTGGCACAG TAAAGACGCA TCGTTCGGTG 
ACCTGGAACC ATGACATCCC CGCGGAGAAG CTGAGCTTCA CCATGCGCCG GGAGTTTGAT 
CGCCAAAGGG AGGAGACTGA GTTGATGTCA CAGCTGAGAA CTATCATTGA GACGCGTCTC 
AAGATGACGT TGCCTGTGGA TATTGCCTCT GCTCTGACGG ATGGGGTTAT ACTGTGTCAT 
TTGGCCAACT ATGTGCGTCC GCGATCAGTG GCCAGCATTC ATGTGCCATC TCCCGGTGTC 
AATAAGTTGA CAATGGCCAG GTGCCGGCGG AATGTGGATA ACTTTCTGGA GGCGTGTCGA 
CGCATTGGCG TAGATGAGGA GTTGATTTGC TCCTGCGAAG ATGTTGTGCC ACAAATTGAA 
CCGCAACAAC CACAATTGCC ACGTGCTGAC GATGACTATG ATGCTGATGT TTATGTCCAA 
GATGATGGCA GCATCAGCCG CAGCAATGAC AGCAGCCACC TGCAATGCAG CGACGATTGC 
AACAGCAGCA GCAACCATAT TAATAGAGTG CCCAATGCAT TGGCCCTACT GCGCACAGTG 
TCCGCTCTGA TTGCCATGGA TGAAAATCCG TATCATCAGC AGCTTCATCA TCATCTTCAG 
CTTCAGCTGG AGCAGCAACA CTTCGAGCAA GAGCAATTCA GTGAGCCAAC TACCTGCAGC 
CAACAGCAAG AACAGATGTT GCTGTTGCAG CAGCAACAGC AGCAGCTAGA GCACGTTTTG 
TATGAGAATG GCCTGCCGAG GGCTGAAAAT GATGATGGGG TTGAGGATCG TGATGATGAT 
ATAGGCAAGC GGCAGCAGCA ACTAGCGACA GTGGGTCAAA TGCTGCTGAA GGCGAAGCAA 
AAGACATATG AAAAAATAAA ACACAATCTG CTAAGTGCCC ATGGTCAGCA TAACTTTCAG 
GGCAATAATA ACAATAGAAT GTTGCATACG ATTGTGGAGA ATGAGCACGA TGTGGCCGCC 
GCAGGACCGG CGGGCCATTA TCAAATAATA GACGAAAAGC AAGAGGAGGA CCCTTACCAG 
CCAAAATCAG AGGACTTGAA GGATGCAGCC GCACTGGGCA ACGCGACCAA GGAAGCCATC 
AGCAAGCGGA TCGAGTTCTT CGAACAGCAG AAGAGTGGAA AACCAAAGCT GGAGGTCTTC 
AGCATCTATT TGCCGAAGGA GAAAACCAAG GAACTCGAGC TTGAGCAGAA AATAAAAGCA 
AAAACCAACG AAGCTGGATC TTCGATATTA AAGCACGATT CCCATACATT GACAGTGATC 
CTGTCATGCG TTTTCATCGC CACCTTCCTC TGGCTGGTAT TTTTCCCGCT GCCTGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Lrch cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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