Cyt-c-d cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cyt-c-d
Entrez Gene ID	34995
Full Name	Cytochrome c distal
Synonyms	CG13263,CYC,Cyt C,Cyt c,Cyt-c1,CytC,CytC-1,CytC1,Cytc,Cytc-d,DC3,Dmel\CG13263,bln,bs30f07.y1,cyt c,cyt c-d,cyt-c-d,cyt.c,cyt.c-d,cytochrome c,dc3,ms(2)04445,pDMc02
General protein information	Preferred Names Cytochrome c distal Names CG13263 gene product from transcript CG13263-RA CG13263-PA CG13263-PB Cyt-c-d-PA Cyt-c-d-PB blanks cytochrome c cytochrome c distal cytochrome c-d cytochrome-c-d
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 36A11-36A11

mRNA and Protein(s)

mRNA	Protein	Name
NM_057816.5	NP_477164.1	cytochrome c distal, isoform A
NM_001273580.1	NP_001260509.1	cytochrome c distal, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We found that cyt c-d--as with ark and dronc-regulates scutellar bristle number, which is known to depend on caspase activity. Collectively, our results indicate a role of Cyt c in caspase regulation of Drosophila somatic cells.
Title: Cytochrome c-d regulates developmental apoptosis in the Drosophila retina.

DARK-mediated DRONC activation occurs independently of Cyt-c-d
Title: The two cytochrome c species, DC3 and DC4, are not required for caspase activation and apoptosis in Drosophila cells.

We further identified loss-of-function mutations in one of the two Drosophila cyt-c genes, cyt-c-d, which block caspase activation and subsequent spermatid terminal differentiation.
Title: Caspase activity and a specific cytochrome C are required for sperm differentiation in Drosophila.

The following Cyt-c-d gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cyt-c-d cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14762	NM_057816.5 Latest version!	Drosophila melanogaster cytochrome c distal, transcript variant A (Cyt-c-d), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa14762	NM_001273580.1 Latest version!	Drosophila melanogaster cytochrome c distal, transcript variant B (Cyt-c-d), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cyt-c-d ( NM_057816.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477164.1 Drosophila melanogaster cytochrome c distal, transcript variant A (Cyt-c-d), mRNA.

Price & Availability↑

CloneID	OFa14762
Clone ID Related Accession (Same CDS sequence)	NM_057816.5 , NM_001273580.1
Accession Version	NM_057816.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cytochrome c distal, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057816.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTTCTG GTGATGCAGA GAACGGCAAG AAGATATTTG TGCAGAAGTG CGCCCAGTGC 
CACACCTACG AAGTGGGCGG CAAACACAAG GTGGGCCCAA ATCTTGGCGG GGTCGTGGGT 
CGCAAGTGTG GCACAGCAGC GGGATACAAG TATACCGATG CCAATATAAA GAAGGGCGTT 
ACCTGGACAG AGGGGAATTT GGACGAGTAC CTCAAGGACC CGAAGAAATA CATTCCCGGA 
ACAAAGATGG TGTTCGCAGG TCTTAAAAAG GCTGAGGAGC GGGCCGATTT GATTGCCTTC 
CTCAAGTCAA ACAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477164.1
CDS	337..654
Translation	MGSGDAENGKKIFVQKCAQCHTYEVGGKHKVGPNLGGVVGRKCGTAAGYKYTDANIKKGVTWTEGNLDEYLKDPKKYIPGTKMVFAGLKKAEERADLIAFLKSNK

Target ORF information:

RefSeq Version	NM_057816.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cytochrome c distal, transcript variant A (Cyt-c-d), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057816.5

ORF Insert Sequence:

ATGGGTTCTG GTGATGCAGA GAACGGCAAG AAGATATTTG TGCAGAAGTG CGCCCAGTGC 
CACACCTACG AAGTGGGCGG CAAACACAAG GTGGGCCCAA ATCTTGGCGG GGTCGTGGGT 
CGCAAGTGTG GCACAGCAGC GGGATACAAG TATACCGATG CCAATATAAA GAAGGGCGTT 
ACCTGGACAG AGGGGAATTT GGACGAGTAC CTCAAGGACC CGAAGAAATA CATTCCCGGA 
ACAAAGATGG TGTTCGCAGG TCTTAAAAAG GCTGAGGAGC GGGCCGATTT GATTGCCTTC 
CTCAAGTCAA ACAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cyt-c-d ( NM_001273580.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260509.1 Drosophila melanogaster cytochrome c distal, transcript variant B (Cyt-c-d), mRNA.

Price & Availability↑

CloneID	OFa14762
Clone ID Related Accession (Same CDS sequence)	NM_057816.5 , NM_001273580.1
Accession Version	NM_001273580.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cytochrome c distal, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTTCTG GTGATGCAGA GAACGGCAAG AAGATATTTG TGCAGAAGTG CGCCCAGTGC 
CACACCTACG AAGTGGGCGG CAAACACAAG GTGGGCCCAA ATCTTGGCGG GGTCGTGGGT 
CGCAAGTGTG GCACAGCAGC GGGATACAAG TATACCGATG CCAATATAAA GAAGGGCGTT 
ACCTGGACAG AGGGGAATTT GGACGAGTAC CTCAAGGACC CGAAGAAATA CATTCCCGGA 
ACAAAGATGG TGTTCGCAGG TCTTAAAAAG GCTGAGGAGC GGGCCGATTT GATTGCCTTC 
CTCAAGTCAA ACAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260509.1
CDS	267..584
Translation	MGSGDAENGKKIFVQKCAQCHTYEVGGKHKVGPNLGGVVGRKCGTAAGYKYTDANIKKGVTWTEGNLDEYLKDPKKYIPGTKMVFAGLKKAEERADLIAFLKSNK

Target ORF information:

RefSeq Version	NM_001273580.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cytochrome c distal, transcript variant B (Cyt-c-d), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273580.1

ORF Insert Sequence:

ATGGGTTCTG GTGATGCAGA GAACGGCAAG AAGATATTTG TGCAGAAGTG CGCCCAGTGC 
CACACCTACG AAGTGGGCGG CAAACACAAG GTGGGCCCAA ATCTTGGCGG GGTCGTGGGT 
CGCAAGTGTG GCACAGCAGC GGGATACAAG TATACCGATG CCAATATAAA GAAGGGCGTT 
ACCTGGACAG AGGGGAATTT GGACGAGTAC CTCAAGGACC CGAAGAAATA CATTCCCGGA 
ACAAAGATGG TGTTCGCAGG TCTTAAAAAG GCTGAGGAGC GGGCCGATTT GATTGCCTTC 
CTCAAGTCAA ACAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Cyt-c-d cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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