Ku80 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ku80
Entrez Gene ID	34930
Full Name	CG18801 gene product from transcript CG18801-RA
Synonyms	BG:DS09217.6,CG18801,DmKu80,Dmel\CG18801,KU80,Ku,ku80
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 35D4-35D4

mRNA and Protein(s)

mRNA	Protein	Name
NM_135923.3	NP_609767.2	Ku80

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Genome-wide gene-set-based analysis and follow-up studies in Drosophila and humans generated independent evidence for the involvement of XRCC5 (Ku80) in alcohol dependence
Title: XRCC5 as a risk gene for alcohol dependence: evidence from a genome-wide gene-set-based analysis and follow-up studies in Drosophila and humans.

specific regulation of ku80 expression by UTX
Title: Drosophila UTX coordinates with p53 to regulate ku80 expression in response to DNA damage.

Role for Ku subunits in the regulation of sperm individualization.
Title: Up-regulation of the Ku heterodimer in Drosophila testicular cyst cells.

Decrease in gene dosage causes a sharp increase in the frequency of HeT-A and TART attachments to a broken chromosome end and in terminal DNA elongation by gene conversion.
Title: The Ku protein complex is involved in length regulation of Drosophila telomeres.

The following Ku80 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ku80 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa16799	NM_135923.3 Latest version!	Drosophila melanogaster Ku80 (Ku80), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ku80 ( NM_135923.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609767.2 Drosophila melanogaster Ku80 (Ku80), mRNA.

Price & Availability↑

CloneID	OFa16799
Clone ID Related Accession (Same CDS sequence)	NM_135923.3
Accession Version	NM_135923.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ku80
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_135923.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTTCTA ACAAGGAATG CCTTATAATT GTACTGGATG TGCGAACATG TGCCGCCGAA 
GAAGTGAAGC TGAAGTCCGC AAAATGTGTG GCGGAGATTC TTAAGGACAA GATCGTGTGC 
GATAGGAAGG ACTACGTGTC CTTCGTCCTC GTGGGTTGCG ACACCGATGA AATAAAAACT 
GAGGATGCTT CCCACCCAAA TGTGCTGCCC TTTGGTGAAC CAAGACTTTG CAGCTGGCAG 
CTCTTGCTGG AATTCTTCCA GTTTGTGAAT AAGACAGCCT GCGAGGATGG AGAATGGCTA 
AATGGTCTAC AGGCGGCCCT GGAGCTTCAG AATGTCGCAA CTACCTTGAG AGTCGCCAGG 
CGGAGGATCC TCCTGCTCTT CGATTTCAAC GACTTTCCAC AGGACTACGA GAAGTTCAAC 
GAAATCACGG ACGAATTACT TGGCGAAAAC ATCGAGTTAA TTGTGGGAAC TCACAACATA 
GCCTACATAG ACAATGCCAT CACTTCTCAG CCTCAGGCTA TTTTCAACTT TTCGCGCAAA 
TGCGGCCCCG ATGAGCTGAA TAACCAGAAG TACGCCCTCA GTCTCGTTCC TCGCTGCAAT 
GCGACGTTGT GCAGCTTCAA GGAGGCACTG CATACCGTCT TCAAGGTGAC CAACCGGCGA 
CCCTGGGTGT GGAACGCAAA GCTGAACATC GGCAGCAAGA TCTCCATTAG CCTGCAGGGC 
ATAATCGCCA TGAAGAACCA GACGCCCGTA AAGCTGGTAA AGGTCTGGGC GGAAAAGGAT 
GAGATAGTGA TACGGGAGAC TCGTCATTAT ATCAAAGGCA CTGAGATCAC CCCGCTGCCG 
GAGAATCTGA TAACCGGTTA CATGCTGGGC GGCACTCCGG TGCCATACGA CGAGGCCGTG 
CTAGAGCCAA AGGAACCACA CCCACCTGGT CTCCACTTCT TTGGCTTTAT CAAAAGAAAT 
GCCGTGCCGG ATGAGTACTT TTGTGGCGAG TCCTTGTACC TCCTGGTGCA TCAGAAGCAC 
AATCAATCTG CCGCCGTGAA GCTGGACGCT CTGGTGCGTG CTCTCGTTTC TTCGGATCGG 
GCCATACTGT GCTGGAAAAT ATATAGCACC AAGTTTAATA GACCGCAAAT GGTTGTGCTA 
CTGCCCCGCT TAGCAGACGA TACCCATCCG GCTACTTTGT ATATGCTGGA AGTTTCCTAT 
ACGTCTCAGC ACCACTTCTG GGACTTTCCG GCACTGCGAA CCACCAAGAC GGAGTGCAGC 
GAGGAACAGC TGAATGCCAT TGATCAGTTA ATCGATAGCA CCGATCTGGA GTGCACACTG 
CGGGACACGC AGCAGCCACG TCCGTGGGCG CAGAATGATC TGCTGCCGTT CGACGCACTT 
CCCAGCATCT TTGAGCAGAA TGTCATGGAC ATCCTAGAGC GAAAAGTTAT CTATGATAAC 
GACAAGGAAG ACAAAATGCT TAAGGACAAA AACTTCGCAG ATGTATTCTG GCGAGTGCCA 
GATCCCTTGG AGGAGAAGTC AAAGAGAGCG GCAGCCATCG TAAAGAAGCT ATTTCCATTG 
CGCTATAGTC GCGCCTGGCA GGAAAAGCTG TTGGCCAAGG AGCAGGCAGA GAATGGTGTG 
GCAGTCAAGT CGGAACCAGC GGAGAAGGAA ATCCCGTTGC CATCAGACGG TGTGGGCTTA 
ATTGATCCAA TCTCTGATTT CAGGCGAGTC CTGGCTAGCG TTCACACAAT CAGTAATGCC 
ACTGAGCGAG ATGCCCGCTT TCAGGCGCTG GCTGCCGATA CCCGCGTGGT GATCATCACA 
CTGCTGCAGC GCAGGAAACA GAACATCGGG CAACTGGGCG AGCTCATCAC CCTGTACCGA 
CAGAGCTGTA TAGACTTCAA CACCTTTTTG GAATACGACA AGTTCGCTGA GGAGCTGAAG 
AAGATCGCGC TGGCCAAGAA TCGCAGCGAA TTCTGGCAAG ACGTTATGGT CGACAAGCAA 
TTGGGCCCTT TGGTGCTTGG CGAGCCGACG CTGGACGACG AATTGGCTCT AAAAGCCTAT 
TACACCATCG AGAATTGGGT GGAGAGTGGG GCGAACGATA TGGAAGACGT TGAGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609767.2
CDS	96..2195
Translation	MASNKECLIIVLDVRTCAAEEVKLKSAKCVAEILKDKIVCDRKDYVSFVLVGCDTDEIKTEDASHPNVLPFGEPRLCSWQLLLEFFQFVNKTACEDGEWLNGLQAALELQNVATTLRVARRRILLLFDFNDFPQDYEKFNEITDELLGENIELIVGTHNIAYIDNAITSQPQAIFNFSRKCGPDELNNQKYALSLVPRCNATLCSFKEALHTVFKVTNRRPWVWNAKLNIGSKISISLQGIIAMKNQTPVKLVKVWAEKDEIVIRETRHYIKGTEITPLPENLITGYMLGGTPVPYDEAVLEPKEPHPPGLHFFGFIKRNAVPDEYFCGESLYLLVHQKHNQSAAVKLDALVRALVSSDRAILCWKIYSTKFNRPQMVVLLPRLADDTHPATLYMLEVSYTSQHHFWDFPALRTTKTECSEEQLNAIDQLIDSTDLECTLRDTQQPRPWAQNDLLPFDALPSIFEQNVMDILERKVIYDNDKEDKMLKDKNFADVFWRVPDPLEEKSKRAAAIVKKLFPLRYSRAWQEKLLAKEQAENGVAVKSEPAEKEIPLPSDGVGLIDPISDFRRVLASVHTISNATERDARFQALAADTRVVIITLLQRRKQNIGQLGELITLYRQSCIDFNTFLEYDKFAEELKKIALAKNRSEFWQDVMVDKQLGPLVLGEPTLDDELALKAYYTIENWVESGANDMEDVEM

Target ORF information:

RefSeq Version	NM_135923.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ku80 (Ku80), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135923.3

ORF Insert Sequence:

ATGGCTTCTA ACAAGGAATG CCTTATAATT GTACTGGATG TGCGAACATG TGCCGCCGAA 
GAAGTGAAGC TGAAGTCCGC AAAATGTGTG GCGGAGATTC TTAAGGACAA GATCGTGTGC 
GATAGGAAGG ACTACGTGTC CTTCGTCCTC GTGGGTTGCG ACACCGATGA AATAAAAACT 
GAGGATGCTT CCCACCCAAA TGTGCTGCCC TTTGGTGAAC CAAGACTTTG CAGCTGGCAG 
CTCTTGCTGG AATTCTTCCA GTTTGTGAAT AAGACAGCCT GCGAGGATGG AGAATGGCTA 
AATGGTCTAC AGGCGGCCCT GGAGCTTCAG AATGTCGCAA CTACCTTGAG AGTCGCCAGG 
CGGAGGATCC TCCTGCTCTT CGATTTCAAC GACTTTCCAC AGGACTACGA GAAGTTCAAC 
GAAATCACGG ACGAATTACT TGGCGAAAAC ATCGAGTTAA TTGTGGGAAC TCACAACATA 
GCCTACATAG ACAATGCCAT CACTTCTCAG CCTCAGGCTA TTTTCAACTT TTCGCGCAAA 
TGCGGCCCCG ATGAGCTGAA TAACCAGAAG TACGCCCTCA GTCTCGTTCC TCGCTGCAAT 
GCGACGTTGT GCAGCTTCAA GGAGGCACTG CATACCGTCT TCAAGGTGAC CAACCGGCGA 
CCCTGGGTGT GGAACGCAAA GCTGAACATC GGCAGCAAGA TCTCCATTAG CCTGCAGGGC 
ATAATCGCCA TGAAGAACCA GACGCCCGTA AAGCTGGTAA AGGTCTGGGC GGAAAAGGAT 
GAGATAGTGA TACGGGAGAC TCGTCATTAT ATCAAAGGCA CTGAGATCAC CCCGCTGCCG 
GAGAATCTGA TAACCGGTTA CATGCTGGGC GGCACTCCGG TGCCATACGA CGAGGCCGTG 
CTAGAGCCAA AGGAACCACA CCCACCTGGT CTCCACTTCT TTGGCTTTAT CAAAAGAAAT 
GCCGTGCCGG ATGAGTACTT TTGTGGCGAG TCCTTGTACC TCCTGGTGCA TCAGAAGCAC 
AATCAATCTG CCGCCGTGAA GCTGGACGCT CTGGTGCGTG CTCTCGTTTC TTCGGATCGG 
GCCATACTGT GCTGGAAAAT ATATAGCACC AAGTTTAATA GACCGCAAAT GGTTGTGCTA 
CTGCCCCGCT TAGCAGACGA TACCCATCCG GCTACTTTGT ATATGCTGGA AGTTTCCTAT 
ACGTCTCAGC ACCACTTCTG GGACTTTCCG GCACTGCGAA CCACCAAGAC GGAGTGCAGC 
GAGGAACAGC TGAATGCCAT TGATCAGTTA ATCGATAGCA CCGATCTGGA GTGCACACTG 
CGGGACACGC AGCAGCCACG TCCGTGGGCG CAGAATGATC TGCTGCCGTT CGACGCACTT 
CCCAGCATCT TTGAGCAGAA TGTCATGGAC ATCCTAGAGC GAAAAGTTAT CTATGATAAC 
GACAAGGAAG ACAAAATGCT TAAGGACAAA AACTTCGCAG ATGTATTCTG GCGAGTGCCA 
GATCCCTTGG AGGAGAAGTC AAAGAGAGCG GCAGCCATCG TAAAGAAGCT ATTTCCATTG 
CGCTATAGTC GCGCCTGGCA GGAAAAGCTG TTGGCCAAGG AGCAGGCAGA GAATGGTGTG 
GCAGTCAAGT CGGAACCAGC GGAGAAGGAA ATCCCGTTGC CATCAGACGG TGTGGGCTTA 
ATTGATCCAA TCTCTGATTT CAGGCGAGTC CTGGCTAGCG TTCACACAAT CAGTAATGCC 
ACTGAGCGAG ATGCCCGCTT TCAGGCGCTG GCTGCCGATA CCCGCGTGGT GATCATCACA 
CTGCTGCAGC GCAGGAAACA GAACATCGGG CAACTGGGCG AGCTCATCAC CCTGTACCGA 
CAGAGCTGTA TAGACTTCAA CACCTTTTTG GAATACGACA AGTTCGCTGA GGAGCTGAAG 
AAGATCGCGC TGGCCAAGAA TCGCAGCGAA TTCTGGCAAG ACGTTATGGT CGACAAGCAA 
TTGGGCCCTT TGGTGCTTGG CGAGCCGACG CTGGACGACG AATTGGCTCT AAAAGCCTAT 
TACACCATCG AGAATTGGGT GGAGAGTGGG GCGAACGATA TGGAAGACGT TGAGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Ku80 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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