kuz cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	kuz
Entrez Gene ID	34772
Full Name	kuzbanian
Synonyms	11410,34Da,ADAM10,BG:DS07660.3,CG7147,CT22079,Dmel\CG7147,GS11410,Kuz,br38,l(2)03782,l(2)34Da,l(2)br38,l(2)c00136,l(2)k01403,l34Da,soy nut
General protein information	Preferred Names kuzbanian Names CG7147 gene product from transcript CG7147-RB CG7147-PA CG7147-PB CG7147-PC CG7147-PF CG7147-PG kuz-PA kuz-PB kuz-PC kuz-PF kuz-PG kuzbaninan lethal (2) c00136
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 34C4-34C6

mRNA and Protein(s)

mRNA	Protein	Name
NM_001103690.3	NP_001097160.2	kuzbanian, isoform F
NM_165047.3	NP_723825.1	kuzbanian, isoform B
NM_057839.5	NP_477187.1	kuzbanian, isoform A
NM_001014481.3	NP_001014481.1	kuzbanian, isoform C
NM_001103689.2	NP_001097159.2	kuzbanian, isoform G

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	sup-17	NP_492377.1
Drosophila melanogaster (fruit fly)	kuz	NP_001097160.2
Danio rerio (zebrafish)	adam10b	NP_956714.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008790	XP_314916.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

indicates that KUZ is required to facilitate a functional Notch signal in the Notch-dependent cell for correct cell fate specification
Title: On the roles of Notch, Delta, kuzbanian, and inscuteable during the development of Drosophila embryonic neuroblast lineages.

Kuz regulates Notch signaling primarily by activating the Notch receptor and has little overall effect on signaling via disabling Delta.
Title: Kuz and TACE can activate Notch independent of ligand.

Notch activation and KUZ expression are restricted to border cells at stage 9 of oogenesis
Title: Spatially localized Kuzbanian required for specific activation of Notch during border cell migration.

Kuzbanian acts during lateral inhibition within the cardiac primordium. Furthermore we discuss a second function of Kuzbanian in heart cell morphogenesis.
Title: The ADAM metalloprotease Kuzbanian is crucial for proper heart formation in Drosophila melanogaster.

KUZ regulates the relay between the G protein-coupled receptors and EGF ereceptors signaling pathways
Title: The metalloprotease Kuzbanian (ADAM10) mediates the transactivation of EGF receptor by G protein-coupled receptors.

The following kuz gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the kuz cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa14671	NM_165047.3 Latest version!	Drosophila melanogaster kuzbanian (kuz), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa14671	NM_057839.5 Latest version!	Drosophila melanogaster kuzbanian (kuz), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa14674	NM_001014481.3 Latest version!	Drosophila melanogaster kuzbanian (kuz), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa21645	NM_001103689.2 Latest version!	Drosophila melanogaster kuzbanian (kuz), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa14673	NM_001103690.3 Latest version!	Drosophila melanogaster kuzbanian (kuz), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

kuz ( NM_165047.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723825.1 Drosophila melanogaster kuzbanian (kuz), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa14671
Clone ID Related Accession (Same CDS sequence)	NM_165047.3 , NM_057839.5
Accession Version	NM_165047.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	kuzbanian, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165047.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCATCAA AATGTGCTTT CAACATTGTA TTCGTATCGA TCATTTTCAT CATCATCGTA 
AATGGTTACG CAAAAGATAT TTCTGGAGTT AAAAGAGGTC ATGAACGACT TAACGAATAC 
ATATCCCACT ATGAAACACT CAACTATGAT CACGAGCACA TCCGAGCTAG TCACAATAGA 
GCGCGACGAT CAGTGACCAA AGATCAATAT GTACATTTAA AGTTTGCATC ACATGGAAGA 
GACTTCCATC TTAGATTAAA ACGTGATTTA AATACATTTA GCAATAAGTT AGACTTTTAT 
GATAGCAAAG GTCCCATTGA TGTCTCCACG GATCATATCT ATGAGGGCGA AGTGATAGGG 
GATCGTAATA GTTATGTATT TGGTTCCATA CACAATGGGG TATTCGAGGG TAAAATTATA 
ACGGAACGTG ATGCCTATTA TGTTGAACAT GCCAAACATT ATTTTCCCAC AAATCGCACG 
GCGACAACAA CACCACCATC GACTTCAACG ACATCCTCAG CAACAACAGC CACAAAAAGC 
ACACAACCAA CACGGCCTTT GGCCAAAAGC AACACCAGTA CTACTGCCGT TAATAGTAAG 
ACAGAAAACT TTATAAAGAA AATTGCTGAA TCCACAACGA CGAGCCAGCA GCTTCCAGAG 
TATACCGAAT CGTCGTCGTC GTCGACAACA ACATTCCCAC CCACAACAGA GTATTTCGAG 
GACGAAAAGG AGCGTAATGC CGAGGACGAA CTTGATTTTC ACTCCATTAT CTACAAGGAG 
TCACATGTCG AGGACGCCTA CGAAAATGTG CGCGAAGGTC ACGTGGCCGG CTGTGGCATC 
ACGGATGAGG TCTCTCAGTG GATGGAGAAC ATACAAAATT CAGCCGTCGA AGAGTTGCCG 
GAGCCCATGT CAAAGGACTA TCAAAAGCTC CACCGGAAGC AGCTGCACAA AAAGTCCGCC 
CCACAGCAAC AACAGCAGCC CCATCCGCCG AAGAAGTACA TCAGCGGGGA TGAGGACTTC 
AAGTATCCCC ACCAGAAGTA CACGAAGGAA GCTAACTTCG CCGAGGGTGC ATTCTACGAT 
CCATCGACCG GACGTCGCCT GGGCTCATCC GCCAACGTGG CCGACTGGCA TCAGCTCGTC 
CACGAGCGCG TCCGCCGCGC CACCGACAAT GGTGCTGGGG ATAGGGGCTC ATCCGGTGGA 
TCTGGACGCG GTCGCGAGGA CAACAAGAAT ACCTGCTCGC TCTACATTCA AACGGATCCA 
TTGATATGGC GCCACATACG CGAAGGCATT GCTGACCACG ATCGTGGACG CAAGTACGAG 
GTGGATGAGA AAACGCGCGA GGAAATCACA TCGTTGATTG CACATCACGT GACGGCCGTT 
AATTACATTT ACCGCAACAC AAAGTTCGAC GGACGCACCG AGCATCGCAA CATACGCTTT 
GAGGTGCAAC GCATTAAGAT CGATGACGAT TCGGCCTGTC GCAATTCCTA CAATGGTCCA 
CACAATGCCT TTTGCAATGA ACACATGGAT GTCTCGAACT TTTTGAATCT GCATTCCCTA 
GAAGATCACT CGGACTTTTG TTTGGCTTAC GTGTTCACCT ACAGAGATTT CACTGGCGGC 
ACTTTGGGTC TGGCCTGGGT GGCCAGTGCG TCGGGAGCCT CTGGTGGAAT TTGCGAGAAG 
TACAAGACGT ACACGGAAAC GGTGGGTGGA CAGTACCAGA GCACCAAGCG ATCACTCAAC 
ACGGGCATCA TCACCTTTGT CAACTACAAC AGCCGGGTGC CGCCGAAAGT GTCGCAGCTT 
ACGTTGGCAC ACGAGATTGG CCACAACTTT GGATCACCTC ACGATTACCC GCAGGAATGT 
CGTCCTGGTG GCCTAAATGG CAATTACATT ATGTTCGCCA GTGCCACCTC CGGTGATAGG 
CCAAATAACT CCAAGTTCTC GCCCTGCTCC ATTCGGAACA TCTCCAATGT CCTTGACGTG 
CTGGTGGGCA ACACGAAGCG CGACTGCTTC AAGGCCTCGG AAGGTGCCTT CTGCGGCAAC 
AAGATCGTGG AGTCTGGCGA GGAATGCGAC TGTGGCTTCA ACGAGGAGGA GTGCAAGGAC 
AAGTGCTGCT ACCCGCGTCT GATCAGCGAG TACGACCAGT CGCTGAACTC CAGTGCCAAG 
GGATGCACGC GCCGCGCCAA GACCCAGTGC TCACCATCGC AGGGTCCGTG CTGTCTGTCC 
AACTCCTGCA CCTTTGTGCC GACGAGCTAC CACCAGAAGT GCAAGGAGGA GACGGAGTGC 
AGCTGGTCGA GCACATGCAA CGGAACCACG GCCGAGTGTC CGGAGCCACG TCATCGCGAT 
GACAAGACCA TGTGCAACAA TGGAACAGCG CTATGCATCC GCGGTGAATG TAGTGGATCG 
CCATGTTTGC TCTGGAATAT GACAAAGTGC TTCCTTACCT CGACCACACT GCCGCACGTG 
AGCAAGCGCA AGTTGTGCGA CTTGGCCTGC CAGGATGGCA ATGACACCTC CACCTGCCGC 
AGCACCAGCG AGTTTGCCGA TAAATATAAT ATTCAAAAGG GTGGTATTAG TCTGCAGCCC 
GGTTCGCCAT GCGATAATTT CCAGGGCTAC TGCGATGTGT TCCTTAAGTG TCGAGCCGTG 
GATGCCGATG GTCCGCTTCT TCGGCTGAAG AATTTGTTGC TCAACCGGAA GACCCTGCAA 
ACGGTGGCCG AGTGGATCGT CGACAATTGG TACCTAGTGG TTCTGATGGG AGTGGCCTTT 
ATTGTGGTCA TGGGTTCGTT CATCAAATGT TGTGCCGTGC ACACGCCCAG TTCCAATCCG 
AAGAAGCGAC GAGCTCGTCG AATCAGCGAA ACTCTAAGAG CACCCATGAA CACGTTGCGT 
AGAATGCAAC GTCATCCCAA TCAGCGAGGA GCAGGTCCTC GAAGCATCCC ACCGCCGGCG 
CATGAGGCGC AGCATTATTC ACGCGGCGGA GATGGTCGCG GCGGCGGCGG TGGAGGCGGA 
GGTCGCCACG GTGGCTCTAG GTCACACCAT CAACAGCATC CGCACGATTG GGATCGTCAT 
CAGGGTGGCC ACTCAATCGT CCCATTGCCC ACCGGCGGCA GCCATTCAAG TCGCAACTCG 
GCGGCGAATC AAGCGAGAAG AAGCGATGGA CGAGGTCCAC GATCCACCAG CAGTGGGCGG 
CCGCAGGCTA TAGCCAGCGG AAGCGGTGCC GCGAGCGGAG CAGCGCGATC TCATGGCGGG 
TACGGAGCCG AACAGGCGAT ACCGGGTTCC ATTGGTGGTG GTGTCCAGGC GGCCATTAGC 
AGCGGCGGTG TGGTGGCTCG GGCCCAGCTG CCGCTGCCAT TGCCGCCGCC AAATGGACAG 
CAGCAAATGC AACAGCAACA ACAACTGCAA CTACAGCAAC CGGCAATTTC GCCGCAGCAG 
CAGCCGCAGC AAGCGTTCTA CACGCCGAAA GAACTACCAC CACGCAATAA GTCCCGATCA 
TCACGTACCA ACAACACCTC CAACACCACA ACCACCACCA ACTCATCCAC AGCGGCAGCC 
GGCAGTGGGT CGGTCTCGGG ACCGGGCTCG GGGGCGGGCA GTAGTAGTAA GAGCAAGAGC 
GGTAAAAGTG CCAAAGCCAA AGACTCAAAG TCGCAAAAAT CGCAGCAGGC CAACAACAGT 
CGCAGCAGCA GCAAGGAAAA GGGCGTCAAG CCAGTGCGCC GAAATATCGT TTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723825.1
CDS	617..4333
Translation	MSSKCAFNIVFVSIIFIIIVNGYAKDISGVKRGHERLNEYISHYETLNYDHEHIRASHNRARRSVTKDQYVHLKFASHGRDFHLRLKRDLNTFSNKLDFYDSKGPIDVSTDHIYEGEVIGDRNSYVFGSIHNGVFEGKIITERDAYYVEHAKHYFPTNRTATTTPPSTSTTSSATTATKSTQPTRPLAKSNTSTTAVNSKTENFIKKIAESTTTSQQLPEYTESSSSSTTTFPPTTEYFEDEKERNAEDELDFHSIIYKESHVEDAYENVREGHVAGCGITDEVSQWMENIQNSAVEELPEPMSKDYQKLHRKQLHKKSAPQQQQQPHPPKKYISGDEDFKYPHQKYTKEANFAEGAFYDPSTGRRLGSSANVADWHQLVHERVRRATDNGAGDRGSSGGSGRGREDNKNTCSLYIQTDPLIWRHIREGIADHDRGRKYEVDEKTREEITSLIAHHVTAVNYIYRNTKFDGRTEHRNIRFEVQRIKIDDDSACRNSYNGPHNAFCNEHMDVSNFLNLHSLEDHSDFCLAYVFTYRDFTGGTLGLAWVASASGASGGICEKYKTYTETVGGQYQSTKRSLNTGIITFVNYNSRVPPKVSQLTLAHEIGHNFGSPHDYPQECRPGGLNGNYIMFASATSGDRPNNSKFSPCSIRNISNVLDVLVGNTKRDCFKASEGAFCGNKIVESGEECDCGFNEEECKDKCCYPRLISEYDQSLNSSAKGCTRRAKTQCSPSQGPCCLSNSCTFVPTSYHQKCKEETECSWSSTCNGTTAECPEPRHRDDKTMCNNGTALCIRGECSGSPCLLWNMTKCFLTSTTLPHVSKRKLCDLACQDGNDTSTCRSTSEFADKYNIQKGGISLQPGSPCDNFQGYCDVFLKCRAVDADGPLLRLKNLLLNRKTLQTVAEWIVDNWYLVVLMGVAFIVVMGSFIKCCAVHTPSSNPKKRRARRISETLRAPMNTLRRMQRHPNQRGAGPRSIPPPAHEAQHYSRGGDGRGGGGGGGGRHGGSRSHHQQHPHDWDRHQGGHSIVPLPTGGSHSSRNSAANQARRSDGRGPRSTSSGRPQAIASGSGAASGAARSHGGYGAEQAIPGSIGGGVQAAISSGGVVARAQLPLPLPPPNGQQQMQQQQQLQLQQPAISPQQQPQQAFYTPKELPPRNKSRSSRTNNTSNTTTTTNSSTAAAGSGSVSGPGSGAGSSSKSKSGKSAKAKDSKSQKSQQANNSRSSSKEKGVKPVRRNIVY

Target ORF information:

RefSeq Version	NM_165047.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster kuzbanian (kuz), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165047.3

ORF Insert Sequence:

ATGTCATCAA AATGTGCTTT CAACATTGTA TTCGTATCGA TCATTTTCAT CATCATCGTA 
AATGGTTACG CAAAAGATAT TTCTGGAGTT AAAAGAGGTC ATGAACGACT TAACGAATAC 
ATATCCCACT ATGAAACACT CAACTATGAT CACGAGCACA TCCGAGCTAG TCACAATAGA 
GCGCGACGAT CAGTGACCAA AGATCAATAT GTACATTTAA AGTTTGCATC ACATGGAAGA 
GACTTCCATC TTAGATTAAA ACGTGATTTA AATACATTTA GCAATAAGTT AGACTTTTAT 
GATAGCAAAG GTCCCATTGA TGTCTCCACG GATCATATCT ATGAGGGCGA AGTGATAGGG 
GATCGTAATA GTTATGTATT TGGTTCCATA CACAATGGGG TATTCGAGGG TAAAATTATA 
ACGGAACGTG ATGCCTATTA TGTTGAACAT GCCAAACATT ATTTTCCCAC AAATCGCACG 
GCGACAACAA CACCACCATC GACTTCAACG ACATCCTCAG CAACAACAGC CACAAAAAGC 
ACACAACCAA CACGGCCTTT GGCCAAAAGC AACACCAGTA CTACTGCCGT TAATAGTAAG 
ACAGAAAACT TTATAAAGAA AATTGCTGAA TCCACAACGA CGAGCCAGCA GCTTCCAGAG 
TATACCGAAT CGTCGTCGTC GTCGACAACA ACATTCCCAC CCACAACAGA GTATTTCGAG 
GACGAAAAGG AGCGTAATGC CGAGGACGAA CTTGATTTTC ACTCCATTAT CTACAAGGAG 
TCACATGTCG AGGACGCCTA CGAAAATGTG CGCGAAGGTC ACGTGGCCGG CTGTGGCATC 
ACGGATGAGG TCTCTCAGTG GATGGAGAAC ATACAAAATT CAGCCGTCGA AGAGTTGCCG 
GAGCCCATGT CAAAGGACTA TCAAAAGCTC CACCGGAAGC AGCTGCACAA AAAGTCCGCC 
CCACAGCAAC AACAGCAGCC CCATCCGCCG AAGAAGTACA TCAGCGGGGA TGAGGACTTC 
AAGTATCCCC ACCAGAAGTA CACGAAGGAA GCTAACTTCG CCGAGGGTGC ATTCTACGAT 
CCATCGACCG GACGTCGCCT GGGCTCATCC GCCAACGTGG CCGACTGGCA TCAGCTCGTC 
CACGAGCGCG TCCGCCGCGC CACCGACAAT GGTGCTGGGG ATAGGGGCTC ATCCGGTGGA 
TCTGGACGCG GTCGCGAGGA CAACAAGAAT ACCTGCTCGC TCTACATTCA AACGGATCCA 
TTGATATGGC GCCACATACG CGAAGGCATT GCTGACCACG ATCGTGGACG CAAGTACGAG 
GTGGATGAGA AAACGCGCGA GGAAATCACA TCGTTGATTG CACATCACGT GACGGCCGTT 
AATTACATTT ACCGCAACAC AAAGTTCGAC GGACGCACCG AGCATCGCAA CATACGCTTT 
GAGGTGCAAC GCATTAAGAT CGATGACGAT TCGGCCTGTC GCAATTCCTA CAATGGTCCA 
CACAATGCCT TTTGCAATGA ACACATGGAT GTCTCGAACT TTTTGAATCT GCATTCCCTA 
GAAGATCACT CGGACTTTTG TTTGGCTTAC GTGTTCACCT ACAGAGATTT CACTGGCGGC 
ACTTTGGGTC TGGCCTGGGT GGCCAGTGCG TCGGGAGCCT CTGGTGGAAT TTGCGAGAAG 
TACAAGACGT ACACGGAAAC GGTGGGTGGA CAGTACCAGA GCACCAAGCG ATCACTCAAC 
ACGGGCATCA TCACCTTTGT CAACTACAAC AGCCGGGTGC CGCCGAAAGT GTCGCAGCTT 
ACGTTGGCAC ACGAGATTGG CCACAACTTT GGATCACCTC ACGATTACCC GCAGGAATGT 
CGTCCTGGTG GCCTAAATGG CAATTACATT ATGTTCGCCA GTGCCACCTC CGGTGATAGG 
CCAAATAACT CCAAGTTCTC GCCCTGCTCC ATTCGGAACA TCTCCAATGT CCTTGACGTG 
CTGGTGGGCA ACACGAAGCG CGACTGCTTC AAGGCCTCGG AAGGTGCCTT CTGCGGCAAC 
AAGATCGTGG AGTCTGGCGA GGAATGCGAC TGTGGCTTCA ACGAGGAGGA GTGCAAGGAC 
AAGTGCTGCT ACCCGCGTCT GATCAGCGAG TACGACCAGT CGCTGAACTC CAGTGCCAAG 
GGATGCACGC GCCGCGCCAA GACCCAGTGC TCACCATCGC AGGGTCCGTG CTGTCTGTCC 
AACTCCTGCA CCTTTGTGCC GACGAGCTAC CACCAGAAGT GCAAGGAGGA GACGGAGTGC 
AGCTGGTCGA GCACATGCAA CGGAACCACG GCCGAGTGTC CGGAGCCACG TCATCGCGAT 
GACAAGACCA TGTGCAACAA TGGAACAGCG CTATGCATCC GCGGTGAATG TAGTGGATCG 
CCATGTTTGC TCTGGAATAT GACAAAGTGC TTCCTTACCT CGACCACACT GCCGCACGTG 
AGCAAGCGCA AGTTGTGCGA CTTGGCCTGC CAGGATGGCA ATGACACCTC CACCTGCCGC 
AGCACCAGCG AGTTTGCCGA TAAATATAAT ATTCAAAAGG GTGGTATTAG TCTGCAGCCC 
GGTTCGCCAT GCGATAATTT CCAGGGCTAC TGCGATGTGT TCCTTAAGTG TCGAGCCGTG 
GATGCCGATG GTCCGCTTCT TCGGCTGAAG AATTTGTTGC TCAACCGGAA GACCCTGCAA 
ACGGTGGCCG AGTGGATCGT CGACAATTGG TACCTAGTGG TTCTGATGGG AGTGGCCTTT 
ATTGTGGTCA TGGGTTCGTT CATCAAATGT TGTGCCGTGC ACACGCCCAG TTCCAATCCG 
AAGAAGCGAC GAGCTCGTCG AATCAGCGAA ACTCTAAGAG CACCCATGAA CACGTTGCGT 
AGAATGCAAC GTCATCCCAA TCAGCGAGGA GCAGGTCCTC GAAGCATCCC ACCGCCGGCG 
CATGAGGCGC AGCATTATTC ACGCGGCGGA GATGGTCGCG GCGGCGGCGG TGGAGGCGGA 
GGTCGCCACG GTGGCTCTAG GTCACACCAT CAACAGCATC CGCACGATTG GGATCGTCAT 
CAGGGTGGCC ACTCAATCGT CCCATTGCCC ACCGGCGGCA GCCATTCAAG TCGCAACTCG 
GCGGCGAATC AAGCGAGAAG AAGCGATGGA CGAGGTCCAC GATCCACCAG CAGTGGGCGG 
CCGCAGGCTA TAGCCAGCGG AAGCGGTGCC GCGAGCGGAG CAGCGCGATC TCATGGCGGG 
TACGGAGCCG AACAGGCGAT ACCGGGTTCC ATTGGTGGTG GTGTCCAGGC GGCCATTAGC 
AGCGGCGGTG TGGTGGCTCG GGCCCAGCTG CCGCTGCCAT TGCCGCCGCC AAATGGACAG 
CAGCAAATGC AACAGCAACA ACAACTGCAA CTACAGCAAC CGGCAATTTC GCCGCAGCAG 
CAGCCGCAGC AAGCGTTCTA CACGCCGAAA GAACTACCAC CACGCAATAA GTCCCGATCA 
TCACGTACCA ACAACACCTC CAACACCACA ACCACCACCA ACTCATCCAC AGCGGCAGCC 
GGCAGTGGGT CGGTCTCGGG ACCGGGCTCG GGGGCGGGCA GTAGTAGTAA GAGCAAGAGC 
GGTAAAAGTG CCAAAGCCAA AGACTCAAAG TCGCAAAAAT CGCAGCAGGC CAACAACAGT 
CGCAGCAGCA GCAAGGAAAA GGGCGTCAAG CCAGTGCGCC GAAATATCGT TTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kuz ( NM_057839.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477187.1 Drosophila melanogaster kuzbanian (kuz), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa14671
Clone ID Related Accession (Same CDS sequence)	NM_165047.3 , NM_057839.5
Accession Version	NM_057839.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	kuzbanian, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_057839.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCATCAA AATGTGCTTT CAACATTGTA TTCGTATCGA TCATTTTCAT CATCATCGTA 
AATGGTTACG CAAAAGATAT TTCTGGAGTT AAAAGAGGTC ATGAACGACT TAACGAATAC 
ATATCCCACT ATGAAACACT CAACTATGAT CACGAGCACA TCCGAGCTAG TCACAATAGA 
GCGCGACGAT CAGTGACCAA AGATCAATAT GTACATTTAA AGTTTGCATC ACATGGAAGA 
GACTTCCATC TTAGATTAAA ACGTGATTTA AATACATTTA GCAATAAGTT AGACTTTTAT 
GATAGCAAAG GTCCCATTGA TGTCTCCACG GATCATATCT ATGAGGGCGA AGTGATAGGG 
GATCGTAATA GTTATGTATT TGGTTCCATA CACAATGGGG TATTCGAGGG TAAAATTATA 
ACGGAACGTG ATGCCTATTA TGTTGAACAT GCCAAACATT ATTTTCCCAC AAATCGCACG 
GCGACAACAA CACCACCATC GACTTCAACG ACATCCTCAG CAACAACAGC CACAAAAAGC 
ACACAACCAA CACGGCCTTT GGCCAAAAGC AACACCAGTA CTACTGCCGT TAATAGTAAG 
ACAGAAAACT TTATAAAGAA AATTGCTGAA TCCACAACGA CGAGCCAGCA GCTTCCAGAG 
TATACCGAAT CGTCGTCGTC GTCGACAACA ACATTCCCAC CCACAACAGA GTATTTCGAG 
GACGAAAAGG AGCGTAATGC CGAGGACGAA CTTGATTTTC ACTCCATTAT CTACAAGGAG 
TCACATGTCG AGGACGCCTA CGAAAATGTG CGCGAAGGTC ACGTGGCCGG CTGTGGCATC 
ACGGATGAGG TCTCTCAGTG GATGGAGAAC ATACAAAATT CAGCCGTCGA AGAGTTGCCG 
GAGCCCATGT CAAAGGACTA TCAAAAGCTC CACCGGAAGC AGCTGCACAA AAAGTCCGCC 
CCACAGCAAC AACAGCAGCC CCATCCGCCG AAGAAGTACA TCAGCGGGGA TGAGGACTTC 
AAGTATCCCC ACCAGAAGTA CACGAAGGAA GCTAACTTCG CCGAGGGTGC ATTCTACGAT 
CCATCGACCG GACGTCGCCT GGGCTCATCC GCCAACGTGG CCGACTGGCA TCAGCTCGTC 
CACGAGCGCG TCCGCCGCGC CACCGACAAT GGTGCTGGGG ATAGGGGCTC ATCCGGTGGA 
TCTGGACGCG GTCGCGAGGA CAACAAGAAT ACCTGCTCGC TCTACATTCA AACGGATCCA 
TTGATATGGC GCCACATACG CGAAGGCATT GCTGACCACG ATCGTGGACG CAAGTACGAG 
GTGGATGAGA AAACGCGCGA GGAAATCACA TCGTTGATTG CACATCACGT GACGGCCGTT 
AATTACATTT ACCGCAACAC AAAGTTCGAC GGACGCACCG AGCATCGCAA CATACGCTTT 
GAGGTGCAAC GCATTAAGAT CGATGACGAT TCGGCCTGTC GCAATTCCTA CAATGGTCCA 
CACAATGCCT TTTGCAATGA ACACATGGAT GTCTCGAACT TTTTGAATCT GCATTCCCTA 
GAAGATCACT CGGACTTTTG TTTGGCTTAC GTGTTCACCT ACAGAGATTT CACTGGCGGC 
ACTTTGGGTC TGGCCTGGGT GGCCAGTGCG TCGGGAGCCT CTGGTGGAAT TTGCGAGAAG 
TACAAGACGT ACACGGAAAC GGTGGGTGGA CAGTACCAGA GCACCAAGCG ATCACTCAAC 
ACGGGCATCA TCACCTTTGT CAACTACAAC AGCCGGGTGC CGCCGAAAGT GTCGCAGCTT 
ACGTTGGCAC ACGAGATTGG CCACAACTTT GGATCACCTC ACGATTACCC GCAGGAATGT 
CGTCCTGGTG GCCTAAATGG CAATTACATT ATGTTCGCCA GTGCCACCTC CGGTGATAGG 
CCAAATAACT CCAAGTTCTC GCCCTGCTCC ATTCGGAACA TCTCCAATGT CCTTGACGTG 
CTGGTGGGCA ACACGAAGCG CGACTGCTTC AAGGCCTCGG AAGGTGCCTT CTGCGGCAAC 
AAGATCGTGG AGTCTGGCGA GGAATGCGAC TGTGGCTTCA ACGAGGAGGA GTGCAAGGAC 
AAGTGCTGCT ACCCGCGTCT GATCAGCGAG TACGACCAGT CGCTGAACTC CAGTGCCAAG 
GGATGCACGC GCCGCGCCAA GACCCAGTGC TCACCATCGC AGGGTCCGTG CTGTCTGTCC 
AACTCCTGCA CCTTTGTGCC GACGAGCTAC CACCAGAAGT GCAAGGAGGA GACGGAGTGC 
AGCTGGTCGA GCACATGCAA CGGAACCACG GCCGAGTGTC CGGAGCCACG TCATCGCGAT 
GACAAGACCA TGTGCAACAA TGGAACAGCG CTATGCATCC GCGGTGAATG TAGTGGATCG 
CCATGTTTGC TCTGGAATAT GACAAAGTGC TTCCTTACCT CGACCACACT GCCGCACGTG 
AGCAAGCGCA AGTTGTGCGA CTTGGCCTGC CAGGATGGCA ATGACACCTC CACCTGCCGC 
AGCACCAGCG AGTTTGCCGA TAAATATAAT ATTCAAAAGG GTGGTATTAG TCTGCAGCCC 
GGTTCGCCAT GCGATAATTT CCAGGGCTAC TGCGATGTGT TCCTTAAGTG TCGAGCCGTG 
GATGCCGATG GTCCGCTTCT TCGGCTGAAG AATTTGTTGC TCAACCGGAA GACCCTGCAA 
ACGGTGGCCG AGTGGATCGT CGACAATTGG TACCTAGTGG TTCTGATGGG AGTGGCCTTT 
ATTGTGGTCA TGGGTTCGTT CATCAAATGT TGTGCCGTGC ACACGCCCAG TTCCAATCCG 
AAGAAGCGAC GAGCTCGTCG AATCAGCGAA ACTCTAAGAG CACCCATGAA CACGTTGCGT 
AGAATGCAAC GTCATCCCAA TCAGCGAGGA GCAGGTCCTC GAAGCATCCC ACCGCCGGCG 
CATGAGGCGC AGCATTATTC ACGCGGCGGA GATGGTCGCG GCGGCGGCGG TGGAGGCGGA 
GGTCGCCACG GTGGCTCTAG GTCACACCAT CAACAGCATC CGCACGATTG GGATCGTCAT 
CAGGGTGGCC ACTCAATCGT CCCATTGCCC ACCGGCGGCA GCCATTCAAG TCGCAACTCG 
GCGGCGAATC AAGCGAGAAG AAGCGATGGA CGAGGTCCAC GATCCACCAG CAGTGGGCGG 
CCGCAGGCTA TAGCCAGCGG AAGCGGTGCC GCGAGCGGAG CAGCGCGATC TCATGGCGGG 
TACGGAGCCG AACAGGCGAT ACCGGGTTCC ATTGGTGGTG GTGTCCAGGC GGCCATTAGC 
AGCGGCGGTG TGGTGGCTCG GGCCCAGCTG CCGCTGCCAT TGCCGCCGCC AAATGGACAG 
CAGCAAATGC AACAGCAACA ACAACTGCAA CTACAGCAAC CGGCAATTTC GCCGCAGCAG 
CAGCCGCAGC AAGCGTTCTA CACGCCGAAA GAACTACCAC CACGCAATAA GTCCCGATCA 
TCACGTACCA ACAACACCTC CAACACCACA ACCACCACCA ACTCATCCAC AGCGGCAGCC 
GGCAGTGGGT CGGTCTCGGG ACCGGGCTCG GGGGCGGGCA GTAGTAGTAA GAGCAAGAGC 
GGTAAAAGTG CCAAAGCCAA AGACTCAAAG TCGCAAAAAT CGCAGCAGGC CAACAACAGT 
CGCAGCAGCA GCAAGGAAAA GGGCGTCAAG CCAGTGCGCC GAAATATCGT TTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477187.1
CDS	744..4460
Translation	MSSKCAFNIVFVSIIFIIIVNGYAKDISGVKRGHERLNEYISHYETLNYDHEHIRASHNRARRSVTKDQYVHLKFASHGRDFHLRLKRDLNTFSNKLDFYDSKGPIDVSTDHIYEGEVIGDRNSYVFGSIHNGVFEGKIITERDAYYVEHAKHYFPTNRTATTTPPSTSTTSSATTATKSTQPTRPLAKSNTSTTAVNSKTENFIKKIAESTTTSQQLPEYTESSSSSTTTFPPTTEYFEDEKERNAEDELDFHSIIYKESHVEDAYENVREGHVAGCGITDEVSQWMENIQNSAVEELPEPMSKDYQKLHRKQLHKKSAPQQQQQPHPPKKYISGDEDFKYPHQKYTKEANFAEGAFYDPSTGRRLGSSANVADWHQLVHERVRRATDNGAGDRGSSGGSGRGREDNKNTCSLYIQTDPLIWRHIREGIADHDRGRKYEVDEKTREEITSLIAHHVTAVNYIYRNTKFDGRTEHRNIRFEVQRIKIDDDSACRNSYNGPHNAFCNEHMDVSNFLNLHSLEDHSDFCLAYVFTYRDFTGGTLGLAWVASASGASGGICEKYKTYTETVGGQYQSTKRSLNTGIITFVNYNSRVPPKVSQLTLAHEIGHNFGSPHDYPQECRPGGLNGNYIMFASATSGDRPNNSKFSPCSIRNISNVLDVLVGNTKRDCFKASEGAFCGNKIVESGEECDCGFNEEECKDKCCYPRLISEYDQSLNSSAKGCTRRAKTQCSPSQGPCCLSNSCTFVPTSYHQKCKEETECSWSSTCNGTTAECPEPRHRDDKTMCNNGTALCIRGECSGSPCLLWNMTKCFLTSTTLPHVSKRKLCDLACQDGNDTSTCRSTSEFADKYNIQKGGISLQPGSPCDNFQGYCDVFLKCRAVDADGPLLRLKNLLLNRKTLQTVAEWIVDNWYLVVLMGVAFIVVMGSFIKCCAVHTPSSNPKKRRARRISETLRAPMNTLRRMQRHPNQRGAGPRSIPPPAHEAQHYSRGGDGRGGGGGGGGRHGGSRSHHQQHPHDWDRHQGGHSIVPLPTGGSHSSRNSAANQARRSDGRGPRSTSSGRPQAIASGSGAASGAARSHGGYGAEQAIPGSIGGGVQAAISSGGVVARAQLPLPLPPPNGQQQMQQQQQLQLQQPAISPQQQPQQAFYTPKELPPRNKSRSSRTNNTSNTTTTTNSSTAAAGSGSVSGPGSGAGSSSKSKSGKSAKAKDSKSQKSQQANNSRSSSKEKGVKPVRRNIVY

Target ORF information:

RefSeq Version	NM_057839.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster kuzbanian (kuz), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057839.5

ORF Insert Sequence:

ATGTCATCAA AATGTGCTTT CAACATTGTA TTCGTATCGA TCATTTTCAT CATCATCGTA 
AATGGTTACG CAAAAGATAT TTCTGGAGTT AAAAGAGGTC ATGAACGACT TAACGAATAC 
ATATCCCACT ATGAAACACT CAACTATGAT CACGAGCACA TCCGAGCTAG TCACAATAGA 
GCGCGACGAT CAGTGACCAA AGATCAATAT GTACATTTAA AGTTTGCATC ACATGGAAGA 
GACTTCCATC TTAGATTAAA ACGTGATTTA AATACATTTA GCAATAAGTT AGACTTTTAT 
GATAGCAAAG GTCCCATTGA TGTCTCCACG GATCATATCT ATGAGGGCGA AGTGATAGGG 
GATCGTAATA GTTATGTATT TGGTTCCATA CACAATGGGG TATTCGAGGG TAAAATTATA 
ACGGAACGTG ATGCCTATTA TGTTGAACAT GCCAAACATT ATTTTCCCAC AAATCGCACG 
GCGACAACAA CACCACCATC GACTTCAACG ACATCCTCAG CAACAACAGC CACAAAAAGC 
ACACAACCAA CACGGCCTTT GGCCAAAAGC AACACCAGTA CTACTGCCGT TAATAGTAAG 
ACAGAAAACT TTATAAAGAA AATTGCTGAA TCCACAACGA CGAGCCAGCA GCTTCCAGAG 
TATACCGAAT CGTCGTCGTC GTCGACAACA ACATTCCCAC CCACAACAGA GTATTTCGAG 
GACGAAAAGG AGCGTAATGC CGAGGACGAA CTTGATTTTC ACTCCATTAT CTACAAGGAG 
TCACATGTCG AGGACGCCTA CGAAAATGTG CGCGAAGGTC ACGTGGCCGG CTGTGGCATC 
ACGGATGAGG TCTCTCAGTG GATGGAGAAC ATACAAAATT CAGCCGTCGA AGAGTTGCCG 
GAGCCCATGT CAAAGGACTA TCAAAAGCTC CACCGGAAGC AGCTGCACAA AAAGTCCGCC 
CCACAGCAAC AACAGCAGCC CCATCCGCCG AAGAAGTACA TCAGCGGGGA TGAGGACTTC 
AAGTATCCCC ACCAGAAGTA CACGAAGGAA GCTAACTTCG CCGAGGGTGC ATTCTACGAT 
CCATCGACCG GACGTCGCCT GGGCTCATCC GCCAACGTGG CCGACTGGCA TCAGCTCGTC 
CACGAGCGCG TCCGCCGCGC CACCGACAAT GGTGCTGGGG ATAGGGGCTC ATCCGGTGGA 
TCTGGACGCG GTCGCGAGGA CAACAAGAAT ACCTGCTCGC TCTACATTCA AACGGATCCA 
TTGATATGGC GCCACATACG CGAAGGCATT GCTGACCACG ATCGTGGACG CAAGTACGAG 
GTGGATGAGA AAACGCGCGA GGAAATCACA TCGTTGATTG CACATCACGT GACGGCCGTT 
AATTACATTT ACCGCAACAC AAAGTTCGAC GGACGCACCG AGCATCGCAA CATACGCTTT 
GAGGTGCAAC GCATTAAGAT CGATGACGAT TCGGCCTGTC GCAATTCCTA CAATGGTCCA 
CACAATGCCT TTTGCAATGA ACACATGGAT GTCTCGAACT TTTTGAATCT GCATTCCCTA 
GAAGATCACT CGGACTTTTG TTTGGCTTAC GTGTTCACCT ACAGAGATTT CACTGGCGGC 
ACTTTGGGTC TGGCCTGGGT GGCCAGTGCG TCGGGAGCCT CTGGTGGAAT TTGCGAGAAG 
TACAAGACGT ACACGGAAAC GGTGGGTGGA CAGTACCAGA GCACCAAGCG ATCACTCAAC 
ACGGGCATCA TCACCTTTGT CAACTACAAC AGCCGGGTGC CGCCGAAAGT GTCGCAGCTT 
ACGTTGGCAC ACGAGATTGG CCACAACTTT GGATCACCTC ACGATTACCC GCAGGAATGT 
CGTCCTGGTG GCCTAAATGG CAATTACATT ATGTTCGCCA GTGCCACCTC CGGTGATAGG 
CCAAATAACT CCAAGTTCTC GCCCTGCTCC ATTCGGAACA TCTCCAATGT CCTTGACGTG 
CTGGTGGGCA ACACGAAGCG CGACTGCTTC AAGGCCTCGG AAGGTGCCTT CTGCGGCAAC 
AAGATCGTGG AGTCTGGCGA GGAATGCGAC TGTGGCTTCA ACGAGGAGGA GTGCAAGGAC 
AAGTGCTGCT ACCCGCGTCT GATCAGCGAG TACGACCAGT CGCTGAACTC CAGTGCCAAG 
GGATGCACGC GCCGCGCCAA GACCCAGTGC TCACCATCGC AGGGTCCGTG CTGTCTGTCC 
AACTCCTGCA CCTTTGTGCC GACGAGCTAC CACCAGAAGT GCAAGGAGGA GACGGAGTGC 
AGCTGGTCGA GCACATGCAA CGGAACCACG GCCGAGTGTC CGGAGCCACG TCATCGCGAT 
GACAAGACCA TGTGCAACAA TGGAACAGCG CTATGCATCC GCGGTGAATG TAGTGGATCG 
CCATGTTTGC TCTGGAATAT GACAAAGTGC TTCCTTACCT CGACCACACT GCCGCACGTG 
AGCAAGCGCA AGTTGTGCGA CTTGGCCTGC CAGGATGGCA ATGACACCTC CACCTGCCGC 
AGCACCAGCG AGTTTGCCGA TAAATATAAT ATTCAAAAGG GTGGTATTAG TCTGCAGCCC 
GGTTCGCCAT GCGATAATTT CCAGGGCTAC TGCGATGTGT TCCTTAAGTG TCGAGCCGTG 
GATGCCGATG GTCCGCTTCT TCGGCTGAAG AATTTGTTGC TCAACCGGAA GACCCTGCAA 
ACGGTGGCCG AGTGGATCGT CGACAATTGG TACCTAGTGG TTCTGATGGG AGTGGCCTTT 
ATTGTGGTCA TGGGTTCGTT CATCAAATGT TGTGCCGTGC ACACGCCCAG TTCCAATCCG 
AAGAAGCGAC GAGCTCGTCG AATCAGCGAA ACTCTAAGAG CACCCATGAA CACGTTGCGT 
AGAATGCAAC GTCATCCCAA TCAGCGAGGA GCAGGTCCTC GAAGCATCCC ACCGCCGGCG 
CATGAGGCGC AGCATTATTC ACGCGGCGGA GATGGTCGCG GCGGCGGCGG TGGAGGCGGA 
GGTCGCCACG GTGGCTCTAG GTCACACCAT CAACAGCATC CGCACGATTG GGATCGTCAT 
CAGGGTGGCC ACTCAATCGT CCCATTGCCC ACCGGCGGCA GCCATTCAAG TCGCAACTCG 
GCGGCGAATC AAGCGAGAAG AAGCGATGGA CGAGGTCCAC GATCCACCAG CAGTGGGCGG 
CCGCAGGCTA TAGCCAGCGG AAGCGGTGCC GCGAGCGGAG CAGCGCGATC TCATGGCGGG 
TACGGAGCCG AACAGGCGAT ACCGGGTTCC ATTGGTGGTG GTGTCCAGGC GGCCATTAGC 
AGCGGCGGTG TGGTGGCTCG GGCCCAGCTG CCGCTGCCAT TGCCGCCGCC AAATGGACAG 
CAGCAAATGC AACAGCAACA ACAACTGCAA CTACAGCAAC CGGCAATTTC GCCGCAGCAG 
CAGCCGCAGC AAGCGTTCTA CACGCCGAAA GAACTACCAC CACGCAATAA GTCCCGATCA 
TCACGTACCA ACAACACCTC CAACACCACA ACCACCACCA ACTCATCCAC AGCGGCAGCC 
GGCAGTGGGT CGGTCTCGGG ACCGGGCTCG GGGGCGGGCA GTAGTAGTAA GAGCAAGAGC 
GGTAAAAGTG CCAAAGCCAA AGACTCAAAG TCGCAAAAAT CGCAGCAGGC CAACAACAGT 
CGCAGCAGCA GCAAGGAAAA GGGCGTCAAG CCAGTGCGCC GAAATATCGT TTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kuz ( NM_001014481.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001014481.1 Drosophila melanogaster kuzbanian (kuz), transcript variant C, mRNA.

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CloneID	OFa14674
Clone ID Related Accession (Same CDS sequence)	NM_001014481.3
Accession Version	NM_001014481.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	kuzbanian, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001014481.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCATCAA AATGTGCTTT CAACATTGTA TTCGTATCGA TCATTTTCAT CATCATCGTA 
AATGGTTACG CAAAAGATAT TTCTGGAGTT AAAAGAGGTC ATGAACGACT TAACGAATAC 
ATATCCCACT ATGAAACACT CAACTATGAT CACGAGCACA TCCGAGCTAG TCACAATAGA 
GCGCGACGAT CAGTGACCAA AGATCAATAT GTACATTTAA AGTTTGCATC ACATGGAAGA 
GACTTCCATC TTAGATTAAA ACGTGATTTA AATACATTTA GCAATAAGTT AGACTTTTAT 
GATAGCAAAG GTCCCATTGA TGTCTCCACG GATCATATCT ATGAGGGCGA AGTGATAGGG 
GATCGTAATA GTTATGTATT TGGTTCCATA CACAATGGGG TATTCGAGGG TAAAATTATA 
ACGGAACGTG ATGCCTATTA TGTTGAACAT GCCAAACATT ATTTTCCCAC AAATCGCACG 
GCGACAACAA CACCACCATC GACTTCAACG ACATCCTCAG CAACAACAGC CACAAAAAGC 
ACACAACCAA CACGGCCTTT GGCCAAAAGC AACACCAGTA CTACTGCCGT TAATAGTAAG 
ACAGAAAACT TTATAAAGAA AATTGCTGAA TCCACAACGA CGAGCCAGCA GCTTCCAGAG 
TATACCGAAT CGTCGTCGTC GTCGACAACA ACATTCCCAC CCACAACAGA GTATTTCGAG 
GACGAAAAGG AGCGTAATGC CGAGGACGAA CTTGATTTTC ACTCCATTAT CTACAAGGAG 
TCACATGTCG AGGACGCCTA CGAAAATGTG CGCGAAGGTC ACGTGGCCGG CTGTGGCATC 
ACGGATGAGG TCTCTCAGTG GATGGAGAAC ATACAAAATT CAGCCGTCGA AGAGTTGCCG 
GAGCCCATGT CAAAGGACTA TCAAAAGCTC CACCGGAAGC AGCTGCACAA AAAGTCCGCC 
CCACAGCAAC AACAGCAGCC CCATCCGCCG AAGAAGTACA TCAGCGGGGA TGAGGACTTC 
AAGTATCCCC ACCAGAAGTA CACGAAGGAA GCTAACTTCG CCGAGGGTGC ATTCTACGAT 
CCATCGACCG GACGTCGCCT GGGCTCATCC GCCAACGTGG CCGACTGGCA TCAGCTCGTC 
CACGAGCGCG TCCGCCGCGC CACCGACAAT GGTGCTGGGG ATAGGGGCTC ATCCGGTGGA 
TCTGGACGCG GTCGCGAGGA CAACAAGAAT ACCTGCTCGC TCTACATTCA AACGGATCCA 
TTGATATGGC GCCACATACG CGAAGGCATT GCTGACCACG ATCGTGGACG CAAGTACGAG 
GTGGATGAGA AAACGCGCGA GGAAATCACA TCGTTGATTG CACATCACGT GACGGCCGTT 
AATTACATTT ACCGCAACAC AAAGTTCGAC GGACGCACCG AGCATCGCAA CATACGCTTT 
GAGGTGCAAC GCATTAAGAT CGATGACGAT TCGGCCTGTC GCAATTCCTA CAATGGTCCA 
CACAATGCCT TTTGCAATGA ACACATGGAT GTCTCGAACT TTTTGAATCT GCATTCCCTA 
GAAGATCACT CGGACTTTTG TTTGGCTTAC GTGTTCACCT ACAGAGATTT CACTGGCGGC 
ACTTTGGGTC TGGCCTGGGT GGCCAGTGCG TCGGGAGCCT CTGGTGGAAT TTGCGAGAAG 
TACAAGACGT ACACGGAAAC GGTGGGTGGA CAGTACCAGA GCACCAAGCG ATCACTCAAC 
ACGGGCATCA TCACCTTTGT CAACTACAAC AGCCGGGTGC CGCCGAAAGT GTCGCAGCTT 
ACGTTGGCAC ACGAGATTGG CCACAACTTT GGATCACCTC ACGATTACCC GCAGGAATGT 
CGTCCTGGTG GCCTAAATGG CAATTACATT ATGTTCGCCA GTGCCACCTC CGGTGATAGG 
CCAAATAACT CCAAGTTCTC GCCCTGCTCC ATTCGGAACA TCTCCAATGT CCTTGACGTG 
CTGGTGGGCA ACACGAAGCG CGACTGCTTC AAGGCCTCGG AAGGTGCCTT CTGCGGCAAC 
AAGATCGTGG AGTCTGGCGA GGAATGCGAC TGTGGCTTCA ACGAGGAGGA GTGCAAGGAC 
AAGTGCTGCT ACCCGCGTCT GATCAGCGAG TACGACCAGT CGCTGAACTC CAGTGCCAAG 
GGATGCACGC GCCGCGCCAA GACCCAGTGC TCACCATCGC AGGGTCCGTG CTGTCTGTCC 
AACTCCTGCA CCTTTGTGCC GACGAGCTAC CACCAGAAGT GCAAGGAGGA GACGGAGTGC 
AGCTGGTCGA GCACATGCAA CGGAACCACG GCCGAGTGTC CGGAGCCACG TCATCGCGAT 
GACAAGACCA TGTGCAACAA TGGAACAGCG CTATGCATCC GCGGTGAATG TAGTGGATCG 
CCATGTTTGC TCTGGAATAT GACAAAGTGC TTCCTTACCT CGACCACACT GCCGCACGTG 
AGCAAGCGCA AGTTGTGCGA CTTGGCCTGC CAGGATGGCA ATGACACCTC CACCTGCCGC 
AGCACCAGCG AGTTTGCCGA TAAATATAAT ATTCAAAAGG GTGGTATTAG TCTGCAGCCC 
GGTTCGCCAT GCGATAATTT CCAGGGCTAC TGCGATGTGT TCCTTAAGTG TCGAGCCGTG 
GATGCCGATG GTCCGCTTCT TCGGCTGAAG AATTTGTTGC TCAACCGGAA GACCCTGCAA 
ACGGTGGCCG AGTGGATCGT CGACAATTGG TACCTAGTGG TTCTGATGGG AGTGGCCTTT 
ATTGTGGTCA TGGGTTCGTT CATCAAATGT TGTGCCGTGC ACACGCCCAG TTCCAATCCG 
AAGAAGCGAC GAGCTCGTCG AATCAGCGAA ACTCTAAGAG CACCCATGAA CACGTTGCGT 
AGAATGCAAC GTCATCCCAA TCAGCGAGGA GCAGGTCCTC GAAGCATCCC ACCGCCGGCG 
CATGAGGCGC AGCATTATTC ACGCGGCGGA GATGGTCGCG GCGGCGGCGG TGGAGGCGGA 
GGTCGCCACG GTGGCTCTAG GTCACACCAT CAACAGCATC CGCACGATTG GGATCGTCAT 
CAGGGTGGCC ACTCAATCGT CCCATTGCCC ACCGGCGGCA GCCATTCAAG TCGCAACTCG 
GCGGCGAATC AAGCGAGAAG AAGCGATGGA CGAGGTCCAC GATCCACCAG CAGTGGGCGG 
CCGCAGGCTA TAGCCAGCGG AAGCGGTGCC GCGAGCGGAG CAGCGCGATC TCATGGCGGG 
CGTCGCGCCG CCTCCGCTAC AAGTAAGCTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014481.1
CDS	617..3889
Translation	MSSKCAFNIVFVSIIFIIIVNGYAKDISGVKRGHERLNEYISHYETLNYDHEHIRASHNRARRSVTKDQYVHLKFASHGRDFHLRLKRDLNTFSNKLDFYDSKGPIDVSTDHIYEGEVIGDRNSYVFGSIHNGVFEGKIITERDAYYVEHAKHYFPTNRTATTTPPSTSTTSSATTATKSTQPTRPLAKSNTSTTAVNSKTENFIKKIAESTTTSQQLPEYTESSSSSTTTFPPTTEYFEDEKERNAEDELDFHSIIYKESHVEDAYENVREGHVAGCGITDEVSQWMENIQNSAVEELPEPMSKDYQKLHRKQLHKKSAPQQQQQPHPPKKYISGDEDFKYPHQKYTKEANFAEGAFYDPSTGRRLGSSANVADWHQLVHERVRRATDNGAGDRGSSGGSGRGREDNKNTCSLYIQTDPLIWRHIREGIADHDRGRKYEVDEKTREEITSLIAHHVTAVNYIYRNTKFDGRTEHRNIRFEVQRIKIDDDSACRNSYNGPHNAFCNEHMDVSNFLNLHSLEDHSDFCLAYVFTYRDFTGGTLGLAWVASASGASGGICEKYKTYTETVGGQYQSTKRSLNTGIITFVNYNSRVPPKVSQLTLAHEIGHNFGSPHDYPQECRPGGLNGNYIMFASATSGDRPNNSKFSPCSIRNISNVLDVLVGNTKRDCFKASEGAFCGNKIVESGEECDCGFNEEECKDKCCYPRLISEYDQSLNSSAKGCTRRAKTQCSPSQGPCCLSNSCTFVPTSYHQKCKEETECSWSSTCNGTTAECPEPRHRDDKTMCNNGTALCIRGECSGSPCLLWNMTKCFLTSTTLPHVSKRKLCDLACQDGNDTSTCRSTSEFADKYNIQKGGISLQPGSPCDNFQGYCDVFLKCRAVDADGPLLRLKNLLLNRKTLQTVAEWIVDNWYLVVLMGVAFIVVMGSFIKCCAVHTPSSNPKKRRARRISETLRAPMNTLRRMQRHPNQRGAGPRSIPPPAHEAQHYSRGGDGRGGGGGGGGRHGGSRSHHQQHPHDWDRHQGGHSIVPLPTGGSHSSRNSAANQARRSDGRGPRSTSSGRPQAIASGSGAASGAARSHGGRRAASATSKL

Target ORF information:

RefSeq Version	NM_001014481.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster kuzbanian (kuz), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014481.3

ORF Insert Sequence:

ATGTCATCAA AATGTGCTTT CAACATTGTA TTCGTATCGA TCATTTTCAT CATCATCGTA 
AATGGTTACG CAAAAGATAT TTCTGGAGTT AAAAGAGGTC ATGAACGACT TAACGAATAC 
ATATCCCACT ATGAAACACT CAACTATGAT CACGAGCACA TCCGAGCTAG TCACAATAGA 
GCGCGACGAT CAGTGACCAA AGATCAATAT GTACATTTAA AGTTTGCATC ACATGGAAGA 
GACTTCCATC TTAGATTAAA ACGTGATTTA AATACATTTA GCAATAAGTT AGACTTTTAT 
GATAGCAAAG GTCCCATTGA TGTCTCCACG GATCATATCT ATGAGGGCGA AGTGATAGGG 
GATCGTAATA GTTATGTATT TGGTTCCATA CACAATGGGG TATTCGAGGG TAAAATTATA 
ACGGAACGTG ATGCCTATTA TGTTGAACAT GCCAAACATT ATTTTCCCAC AAATCGCACG 
GCGACAACAA CACCACCATC GACTTCAACG ACATCCTCAG CAACAACAGC CACAAAAAGC 
ACACAACCAA CACGGCCTTT GGCCAAAAGC AACACCAGTA CTACTGCCGT TAATAGTAAG 
ACAGAAAACT TTATAAAGAA AATTGCTGAA TCCACAACGA CGAGCCAGCA GCTTCCAGAG 
TATACCGAAT CGTCGTCGTC GTCGACAACA ACATTCCCAC CCACAACAGA GTATTTCGAG 
GACGAAAAGG AGCGTAATGC CGAGGACGAA CTTGATTTTC ACTCCATTAT CTACAAGGAG 
TCACATGTCG AGGACGCCTA CGAAAATGTG CGCGAAGGTC ACGTGGCCGG CTGTGGCATC 
ACGGATGAGG TCTCTCAGTG GATGGAGAAC ATACAAAATT CAGCCGTCGA AGAGTTGCCG 
GAGCCCATGT CAAAGGACTA TCAAAAGCTC CACCGGAAGC AGCTGCACAA AAAGTCCGCC 
CCACAGCAAC AACAGCAGCC CCATCCGCCG AAGAAGTACA TCAGCGGGGA TGAGGACTTC 
AAGTATCCCC ACCAGAAGTA CACGAAGGAA GCTAACTTCG CCGAGGGTGC ATTCTACGAT 
CCATCGACCG GACGTCGCCT GGGCTCATCC GCCAACGTGG CCGACTGGCA TCAGCTCGTC 
CACGAGCGCG TCCGCCGCGC CACCGACAAT GGTGCTGGGG ATAGGGGCTC ATCCGGTGGA 
TCTGGACGCG GTCGCGAGGA CAACAAGAAT ACCTGCTCGC TCTACATTCA AACGGATCCA 
TTGATATGGC GCCACATACG CGAAGGCATT GCTGACCACG ATCGTGGACG CAAGTACGAG 
GTGGATGAGA AAACGCGCGA GGAAATCACA TCGTTGATTG CACATCACGT GACGGCCGTT 
AATTACATTT ACCGCAACAC AAAGTTCGAC GGACGCACCG AGCATCGCAA CATACGCTTT 
GAGGTGCAAC GCATTAAGAT CGATGACGAT TCGGCCTGTC GCAATTCCTA CAATGGTCCA 
CACAATGCCT TTTGCAATGA ACACATGGAT GTCTCGAACT TTTTGAATCT GCATTCCCTA 
GAAGATCACT CGGACTTTTG TTTGGCTTAC GTGTTCACCT ACAGAGATTT CACTGGCGGC 
ACTTTGGGTC TGGCCTGGGT GGCCAGTGCG TCGGGAGCCT CTGGTGGAAT TTGCGAGAAG 
TACAAGACGT ACACGGAAAC GGTGGGTGGA CAGTACCAGA GCACCAAGCG ATCACTCAAC 
ACGGGCATCA TCACCTTTGT CAACTACAAC AGCCGGGTGC CGCCGAAAGT GTCGCAGCTT 
ACGTTGGCAC ACGAGATTGG CCACAACTTT GGATCACCTC ACGATTACCC GCAGGAATGT 
CGTCCTGGTG GCCTAAATGG CAATTACATT ATGTTCGCCA GTGCCACCTC CGGTGATAGG 
CCAAATAACT CCAAGTTCTC GCCCTGCTCC ATTCGGAACA TCTCCAATGT CCTTGACGTG 
CTGGTGGGCA ACACGAAGCG CGACTGCTTC AAGGCCTCGG AAGGTGCCTT CTGCGGCAAC 
AAGATCGTGG AGTCTGGCGA GGAATGCGAC TGTGGCTTCA ACGAGGAGGA GTGCAAGGAC 
AAGTGCTGCT ACCCGCGTCT GATCAGCGAG TACGACCAGT CGCTGAACTC CAGTGCCAAG 
GGATGCACGC GCCGCGCCAA GACCCAGTGC TCACCATCGC AGGGTCCGTG CTGTCTGTCC 
AACTCCTGCA CCTTTGTGCC GACGAGCTAC CACCAGAAGT GCAAGGAGGA GACGGAGTGC 
AGCTGGTCGA GCACATGCAA CGGAACCACG GCCGAGTGTC CGGAGCCACG TCATCGCGAT 
GACAAGACCA TGTGCAACAA TGGAACAGCG CTATGCATCC GCGGTGAATG TAGTGGATCG 
CCATGTTTGC TCTGGAATAT GACAAAGTGC TTCCTTACCT CGACCACACT GCCGCACGTG 
AGCAAGCGCA AGTTGTGCGA CTTGGCCTGC CAGGATGGCA ATGACACCTC CACCTGCCGC 
AGCACCAGCG AGTTTGCCGA TAAATATAAT ATTCAAAAGG GTGGTATTAG TCTGCAGCCC 
GGTTCGCCAT GCGATAATTT CCAGGGCTAC TGCGATGTGT TCCTTAAGTG TCGAGCCGTG 
GATGCCGATG GTCCGCTTCT TCGGCTGAAG AATTTGTTGC TCAACCGGAA GACCCTGCAA 
ACGGTGGCCG AGTGGATCGT CGACAATTGG TACCTAGTGG TTCTGATGGG AGTGGCCTTT 
ATTGTGGTCA TGGGTTCGTT CATCAAATGT TGTGCCGTGC ACACGCCCAG TTCCAATCCG 
AAGAAGCGAC GAGCTCGTCG AATCAGCGAA ACTCTAAGAG CACCCATGAA CACGTTGCGT 
AGAATGCAAC GTCATCCCAA TCAGCGAGGA GCAGGTCCTC GAAGCATCCC ACCGCCGGCG 
CATGAGGCGC AGCATTATTC ACGCGGCGGA GATGGTCGCG GCGGCGGCGG TGGAGGCGGA 
GGTCGCCACG GTGGCTCTAG GTCACACCAT CAACAGCATC CGCACGATTG GGATCGTCAT 
CAGGGTGGCC ACTCAATCGT CCCATTGCCC ACCGGCGGCA GCCATTCAAG TCGCAACTCG 
GCGGCGAATC AAGCGAGAAG AAGCGATGGA CGAGGTCCAC GATCCACCAG CAGTGGGCGG 
CCGCAGGCTA TAGCCAGCGG AAGCGGTGCC GCGAGCGGAG CAGCGCGATC TCATGGCGGG 
CGTCGCGCCG CCTCCGCTAC AAGTAAGCTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kuz ( NM_001103689.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097159.2 Drosophila melanogaster kuzbanian (kuz), transcript variant G, mRNA.

Price & Availability↑

CloneID	OFa21645
Clone ID Related Accession (Same CDS sequence)	NM_001103689.2
Accession Version	NM_001103689.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	kuzbanian, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001103689.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCATCAA AATGTGCTTT CAACATTGTA TTCGTATCGA TCATTTTCAT CATCATCGTA 
AATGGTTACG CAAAAGATAT TTCTGGAGTT AAAAGAGGTC ATGAACGACT TAACGAATAC 
ATATCCCACT ATGAAACACT CAACTATGAT CACGAGCACA TCCGAGCTAG TCACAATAGA 
GCGCGACGAT CAGTGACCAA AGATCAATAT GTACATTTAA AGTTTGCATC ACATGGAAGA 
GACTTCCATC TTAGATTAAA ACGTGATTTA AATACATTTA GCAATAAGTT AGACTTTTAT 
GATAGCAAAG GTCCCATTGA TGTCTCCACG GATCATATCT ATGAGGGCGA AGTGATAGGG 
GATCGTAATA GTTATGTATT TGGTTCCATA CACAATGGGG TATTCGAGGG TAAAATTATA 
ACGGAACGTG ATGCCTATTA TGTTGAACAT GCCAAACATT ATTTTCCCAC AAATCGCACG 
GCGACAACAA CACCACCATC GACTTCAACG ACATCCTCAG CAACAACAGC CACAAAAAGC 
ACACAACCAA CACGGCCTTT GGCCAAAAGC AACACCAGTA CTACTGCCGT TAATAGTAAG 
ACAGAAAACT TTATAAAGAA AATTGCTGAA TCCACAACGA CGAGCCAGCA GCTTCCAGAG 
TATACCGAAT CGTCGTCGTC GTCGACAACA ACATTCCCAC CCACAACAGA GTATTTCGAG 
GACGAAAAGG AGCGTAATGC CGAGGACGAA CTTGATTTTC ACTCCATTAT CTACAAGGAG 
TCACATGTCG AGGACGCCTA CGAAAATGTG CGCGAAGGTC ACGTGGCCGG CTGTGGCATC 
ACGGATGAGG TCTCTCAGTG GATGGAGAAC ATACAAAATT CAGCCGTCGA AGAGTTGCCG 
GAGCCCATGT CAAAGGACTA TCAAAAGCTC CACCGGAAGC AGCTGCACAA AAAGTCCGCC 
CCACAGCAAC AACAGCAGCC CCATCCGCCG AAGAAGTACA TCAGCGGGGA TGAGGACTTC 
AAGTATCCCC ACCAGAAGTA CACGAAGGAA GCTAACTTCG CCGAGGGTGC ATTCTACGAT 
CCATCGACCG GACGTCGCCT GGGCTCATCC GCCAACGTGG CCGACTGGCA TCAGCTCGTC 
CACGAGCGCG TCCGCCGCGC CACCGACAAT GGTGCTGGGG ATAGGGGCTC ATCCGGTGGA 
TCTGGACGCG GTCGCGAGGA CAACAAGAAT ACCTGCTCGC TCTACATTCA AACGGATCCA 
TTGATATGGC GCCACATACG CGAAGGCATT GCTGACCACG ATCGTGGACG CAAGTACGAG 
GTGGATGAGA AAACGCGCGA GGAAATCACA TCGTTGATTG CACATCACGT GACGGCCGTT 
AATTACATTT ACCGCAACAC AAAGTTCGAC GGACGCACCG AGCATCGCAA CATACGCTTT 
GAGGTGCAAC GCATTAAGAT CGATGACGAT TCGGCCTGTC GCAATTCCTA CAATGGTCCA 
CACAATGCCT TTTGCAATGA ACACATGGAT GTCTCGAACT TTTTGAATCT GCATTCCCTA 
GAAGATCACT CGGACTTTTG TTTGGCTTAC GTGTTCACCT ACAGAGATTT CACTGGCGGC 
ACTTTGGGTC TGGCCTGGGT GGCCAGTGCG TCGGGAGCCT CTGGTGGAAT TTGCGAGAAG 
TACAAGACGT ACACGGAAAC GGTGGGTGGA CAGTACCAGA GCACCAAGCG ATCACTCAAC 
ACGGGCATCA TCACCTTTGT CAACTACAAC AGCCGGGTGC CGCCGAAAGT GTCGCAGCTT 
ACGTTGGCAC ACGAGATTGG CCACAACTTT GGATCACCTC ACGATTACCC GCAGGAATGT 
CGTCCTGGTG GCCTAAATGG CAATTACATT ATGTTCGCCA GTGCCACCTC CGGTGATAGG 
CCAAATAACT CCAAGTTCTC GCCCTGCTCC ATTCGGAACA TCTCCAATGT CCTTGACGTG 
CTGGTGGGCA ACACGAAGCG CGACTGCTTC AAGGCCTCGG AAGGTGCCTT CTGCGGCAAC 
AAGATCGTGG AGTCTGGCGA GGAATGCGAC TGTGGCTTCA ACGAGGAGGA GTGCAAGGAC 
AAGTGCTGCT ACCCGCGTCT GATCAGCGAG TACGACCAGT CGCTGAACTC CAGTGCCAAG 
GGATGCACGC GCCGCGCCAA GACCCAGTGC TCACCATCGC AGGGTCCGTG CTGTCTGTCC 
AACTCCTGCA CCTTTGTGCC GACGAGCTAC CACCAGAAGT GCAAGGAGGA GACGGAGTGC 
AGCTGGTCGA GCACATGCAA CGGAACCACG GCCGAGTGTC CGGAGCCACG TCATCGCGAT 
GACAAGACCA TGTGCAACAA TGGAACAGCG CTATGCATCC GCGGTGAATG TAGTGGATCG 
CCATGTTTGC TCTGGAATAT GACAAAGTGC TTCCTTACCT CGACCACACT GCCGCACGTG 
AGCAAGCGCA AGTTGTGCGA CTTGGCCTGC CAGGATGGCA ATGACACCTC CACCTGCCGC 
AGCACCAGCG AGTTTGCCGA TAAATATAAT ATTCAAAAGG GTGGTATTAG TCTGCAGCCC 
GGTTCGCCAT GCGATAATTT CCAGGGCTAC TGCGATGTGT TCCTTAAGTG TCGAGCCGTG 
GATGCCGATG GTCCGCTTCT TCGGCTGAAG AATTTGTTGC TCAACCGGAA GACCCTGCAA 
ACGGTGGCCG AGTGGATCGT CGACAATTGG TACCTAGTGG TTCTGATGGG AGTGGCCTTT 
ATTGTGGTCA TGGGTTCGTT CATCAAATGT TGTGCCGTGC ACACGCCCAG TTCCAATCCG 
AAGAAGCGAC GAGCTCGTCG AATCAGCGAA ACTCTAAGAG CACCCATGAA CACGTTGCGT 
AGAATGCAAC GTCATCCCAA TCAGCGAGGA GCAGGTCCTC GAAGCATCCC ACCGCCGGCG 
CATGAGGCGC AGCATTATTC ACGCGGCGGA GATGGTCGCG GCGGCGGCGG TGGAGGCGGA 
GGTCGCCACG GTGGCTCTAG GTCACACCAT CAACAGCATC CGCACGATTG GGATCGTCAT 
CAGGGTGGCC ACTCAATCGT CCCATTGCCC ACCGGCGGCA GCCATTCAAG TCGCAACTCG 
GCGGCGAATC AAGCGAGAAG AAGCGATGGA CGAGGTCCAC GATCCACCAG CAGTGGGCGG 
CCGCAGGCTA TAGCCAGCGG AAGCGGTGCC GCGAGCGGAG CAGCGCGATC TCATGGCGGG 
TACGGAGCCG AACAGGCGAT ACCGGGTTCC ATTGGTGGTG GTGTCCAGGC GGCCATTAGC 
AGCGGCGGTG TGGTGGCTCG GGCCCAGCTG CCGCTGCCAT TGCCGCCGCC AAATGGACAG 
CAGCAAATGC AACAGCAACA ACAACTGCAA CTACAGCAAC CGGCAATTTC GCCGCAGCAG 
CAGCCGCAGC AAGCGTTCTA CACGCCGAAA GGTGTGTGTC CCATTTCCCT GCAAGTAGAA 
AGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097159.2
CDS	744..4229
Translation	MSSKCAFNIVFVSIIFIIIVNGYAKDISGVKRGHERLNEYISHYETLNYDHEHIRASHNRARRSVTKDQYVHLKFASHGRDFHLRLKRDLNTFSNKLDFYDSKGPIDVSTDHIYEGEVIGDRNSYVFGSIHNGVFEGKIITERDAYYVEHAKHYFPTNRTATTTPPSTSTTSSATTATKSTQPTRPLAKSNTSTTAVNSKTENFIKKIAESTTTSQQLPEYTESSSSSTTTFPPTTEYFEDEKERNAEDELDFHSIIYKESHVEDAYENVREGHVAGCGITDEVSQWMENIQNSAVEELPEPMSKDYQKLHRKQLHKKSAPQQQQQPHPPKKYISGDEDFKYPHQKYTKEANFAEGAFYDPSTGRRLGSSANVADWHQLVHERVRRATDNGAGDRGSSGGSGRGREDNKNTCSLYIQTDPLIWRHIREGIADHDRGRKYEVDEKTREEITSLIAHHVTAVNYIYRNTKFDGRTEHRNIRFEVQRIKIDDDSACRNSYNGPHNAFCNEHMDVSNFLNLHSLEDHSDFCLAYVFTYRDFTGGTLGLAWVASASGASGGICEKYKTYTETVGGQYQSTKRSLNTGIITFVNYNSRVPPKVSQLTLAHEIGHNFGSPHDYPQECRPGGLNGNYIMFASATSGDRPNNSKFSPCSIRNISNVLDVLVGNTKRDCFKASEGAFCGNKIVESGEECDCGFNEEECKDKCCYPRLISEYDQSLNSSAKGCTRRAKTQCSPSQGPCCLSNSCTFVPTSYHQKCKEETECSWSSTCNGTTAECPEPRHRDDKTMCNNGTALCIRGECSGSPCLLWNMTKCFLTSTTLPHVSKRKLCDLACQDGNDTSTCRSTSEFADKYNIQKGGISLQPGSPCDNFQGYCDVFLKCRAVDADGPLLRLKNLLLNRKTLQTVAEWIVDNWYLVVLMGVAFIVVMGSFIKCCAVHTPSSNPKKRRARRISETLRAPMNTLRRMQRHPNQRGAGPRSIPPPAHEAQHYSRGGDGRGGGGGGGGRHGGSRSHHQQHPHDWDRHQGGHSIVPLPTGGSHSSRNSAANQARRSDGRGPRSTSSGRPQAIASGSGAASGAARSHGGYGAEQAIPGSIGGGVQAAISSGGVVARAQLPLPLPPPNGQQQMQQQQQLQLQQPAISPQQQPQQAFYTPKGVCPISLQVER

Target ORF information:

RefSeq Version	NM_001103689.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster kuzbanian (kuz), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103689.2

ORF Insert Sequence:

ATGTCATCAA AATGTGCTTT CAACATTGTA TTCGTATCGA TCATTTTCAT CATCATCGTA 
AATGGTTACG CAAAAGATAT TTCTGGAGTT AAAAGAGGTC ATGAACGACT TAACGAATAC 
ATATCCCACT ATGAAACACT CAACTATGAT CACGAGCACA TCCGAGCTAG TCACAATAGA 
GCGCGACGAT CAGTGACCAA AGATCAATAT GTACATTTAA AGTTTGCATC ACATGGAAGA 
GACTTCCATC TTAGATTAAA ACGTGATTTA AATACATTTA GCAATAAGTT AGACTTTTAT 
GATAGCAAAG GTCCCATTGA TGTCTCCACG GATCATATCT ATGAGGGCGA AGTGATAGGG 
GATCGTAATA GTTATGTATT TGGTTCCATA CACAATGGGG TATTCGAGGG TAAAATTATA 
ACGGAACGTG ATGCCTATTA TGTTGAACAT GCCAAACATT ATTTTCCCAC AAATCGCACG 
GCGACAACAA CACCACCATC GACTTCAACG ACATCCTCAG CAACAACAGC CACAAAAAGC 
ACACAACCAA CACGGCCTTT GGCCAAAAGC AACACCAGTA CTACTGCCGT TAATAGTAAG 
ACAGAAAACT TTATAAAGAA AATTGCTGAA TCCACAACGA CGAGCCAGCA GCTTCCAGAG 
TATACCGAAT CGTCGTCGTC GTCGACAACA ACATTCCCAC CCACAACAGA GTATTTCGAG 
GACGAAAAGG AGCGTAATGC CGAGGACGAA CTTGATTTTC ACTCCATTAT CTACAAGGAG 
TCACATGTCG AGGACGCCTA CGAAAATGTG CGCGAAGGTC ACGTGGCCGG CTGTGGCATC 
ACGGATGAGG TCTCTCAGTG GATGGAGAAC ATACAAAATT CAGCCGTCGA AGAGTTGCCG 
GAGCCCATGT CAAAGGACTA TCAAAAGCTC CACCGGAAGC AGCTGCACAA AAAGTCCGCC 
CCACAGCAAC AACAGCAGCC CCATCCGCCG AAGAAGTACA TCAGCGGGGA TGAGGACTTC 
AAGTATCCCC ACCAGAAGTA CACGAAGGAA GCTAACTTCG CCGAGGGTGC ATTCTACGAT 
CCATCGACCG GACGTCGCCT GGGCTCATCC GCCAACGTGG CCGACTGGCA TCAGCTCGTC 
CACGAGCGCG TCCGCCGCGC CACCGACAAT GGTGCTGGGG ATAGGGGCTC ATCCGGTGGA 
TCTGGACGCG GTCGCGAGGA CAACAAGAAT ACCTGCTCGC TCTACATTCA AACGGATCCA 
TTGATATGGC GCCACATACG CGAAGGCATT GCTGACCACG ATCGTGGACG CAAGTACGAG 
GTGGATGAGA AAACGCGCGA GGAAATCACA TCGTTGATTG CACATCACGT GACGGCCGTT 
AATTACATTT ACCGCAACAC AAAGTTCGAC GGACGCACCG AGCATCGCAA CATACGCTTT 
GAGGTGCAAC GCATTAAGAT CGATGACGAT TCGGCCTGTC GCAATTCCTA CAATGGTCCA 
CACAATGCCT TTTGCAATGA ACACATGGAT GTCTCGAACT TTTTGAATCT GCATTCCCTA 
GAAGATCACT CGGACTTTTG TTTGGCTTAC GTGTTCACCT ACAGAGATTT CACTGGCGGC 
ACTTTGGGTC TGGCCTGGGT GGCCAGTGCG TCGGGAGCCT CTGGTGGAAT TTGCGAGAAG 
TACAAGACGT ACACGGAAAC GGTGGGTGGA CAGTACCAGA GCACCAAGCG ATCACTCAAC 
ACGGGCATCA TCACCTTTGT CAACTACAAC AGCCGGGTGC CGCCGAAAGT GTCGCAGCTT 
ACGTTGGCAC ACGAGATTGG CCACAACTTT GGATCACCTC ACGATTACCC GCAGGAATGT 
CGTCCTGGTG GCCTAAATGG CAATTACATT ATGTTCGCCA GTGCCACCTC CGGTGATAGG 
CCAAATAACT CCAAGTTCTC GCCCTGCTCC ATTCGGAACA TCTCCAATGT CCTTGACGTG 
CTGGTGGGCA ACACGAAGCG CGACTGCTTC AAGGCCTCGG AAGGTGCCTT CTGCGGCAAC 
AAGATCGTGG AGTCTGGCGA GGAATGCGAC TGTGGCTTCA ACGAGGAGGA GTGCAAGGAC 
AAGTGCTGCT ACCCGCGTCT GATCAGCGAG TACGACCAGT CGCTGAACTC CAGTGCCAAG 
GGATGCACGC GCCGCGCCAA GACCCAGTGC TCACCATCGC AGGGTCCGTG CTGTCTGTCC 
AACTCCTGCA CCTTTGTGCC GACGAGCTAC CACCAGAAGT GCAAGGAGGA GACGGAGTGC 
AGCTGGTCGA GCACATGCAA CGGAACCACG GCCGAGTGTC CGGAGCCACG TCATCGCGAT 
GACAAGACCA TGTGCAACAA TGGAACAGCG CTATGCATCC GCGGTGAATG TAGTGGATCG 
CCATGTTTGC TCTGGAATAT GACAAAGTGC TTCCTTACCT CGACCACACT GCCGCACGTG 
AGCAAGCGCA AGTTGTGCGA CTTGGCCTGC CAGGATGGCA ATGACACCTC CACCTGCCGC 
AGCACCAGCG AGTTTGCCGA TAAATATAAT ATTCAAAAGG GTGGTATTAG TCTGCAGCCC 
GGTTCGCCAT GCGATAATTT CCAGGGCTAC TGCGATGTGT TCCTTAAGTG TCGAGCCGTG 
GATGCCGATG GTCCGCTTCT TCGGCTGAAG AATTTGTTGC TCAACCGGAA GACCCTGCAA 
ACGGTGGCCG AGTGGATCGT CGACAATTGG TACCTAGTGG TTCTGATGGG AGTGGCCTTT 
ATTGTGGTCA TGGGTTCGTT CATCAAATGT TGTGCCGTGC ACACGCCCAG TTCCAATCCG 
AAGAAGCGAC GAGCTCGTCG AATCAGCGAA ACTCTAAGAG CACCCATGAA CACGTTGCGT 
AGAATGCAAC GTCATCCCAA TCAGCGAGGA GCAGGTCCTC GAAGCATCCC ACCGCCGGCG 
CATGAGGCGC AGCATTATTC ACGCGGCGGA GATGGTCGCG GCGGCGGCGG TGGAGGCGGA 
GGTCGCCACG GTGGCTCTAG GTCACACCAT CAACAGCATC CGCACGATTG GGATCGTCAT 
CAGGGTGGCC ACTCAATCGT CCCATTGCCC ACCGGCGGCA GCCATTCAAG TCGCAACTCG 
GCGGCGAATC AAGCGAGAAG AAGCGATGGA CGAGGTCCAC GATCCACCAG CAGTGGGCGG 
CCGCAGGCTA TAGCCAGCGG AAGCGGTGCC GCGAGCGGAG CAGCGCGATC TCATGGCGGG 
TACGGAGCCG AACAGGCGAT ACCGGGTTCC ATTGGTGGTG GTGTCCAGGC GGCCATTAGC 
AGCGGCGGTG TGGTGGCTCG GGCCCAGCTG CCGCTGCCAT TGCCGCCGCC AAATGGACAG 
CAGCAAATGC AACAGCAACA ACAACTGCAA CTACAGCAAC CGGCAATTTC GCCGCAGCAG 
CAGCCGCAGC AAGCGTTCTA CACGCCGAAA GGTGTGTGTC CCATTTCCCT GCAAGTAGAA 
AGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kuz ( NM_001103690.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097160.2 Drosophila melanogaster kuzbanian (kuz), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa14673
Clone ID Related Accession (Same CDS sequence)	NM_001103690.3
Accession Version	NM_001103690.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	kuzbanian, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001103690.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCATCAA AATGTGCTTT CAACATTGTA TTCGTATCGA TCATTTTCAT CATCATCGTA 
AATGGTTACG CAAAAGATAT TTCTGGAGTT AAAAGAGGTC ATGAACGACT TAACGAATAC 
ATATCCCACT ATGAAACACT CAACTATGAT CACGAGCACA TCCGAGCTAG TCACAATAGA 
GCGCGACGAT CAGTGACCAA AGATCAATAT GTACATTTAA AGTTTGCATC ACATGGAAGA 
GACTTCCATC TTAGATTAAA ACGTGATTTA AATACATTTA GCAATAAGTT AGACTTTTAT 
GATAGCAAAG GTCCCATTGA TGTCTCCACG GATCATATCT ATGAGGGCGA AGTGATAGGG 
GATCGTAATA GTTATGTATT TGGTTCCATA CACAATGGGG TATTCGAGGG TAAAATTATA 
ACGGAACGTG ATGCCTATTA TGTTGAACAT GCCAAACATT ATTTTCCCAC AAATCGCACG 
GCGACAACAA CACCACCATC GACTTCAACG ACATCCTCAG CAACAACAGC CACAAAAAGC 
ACACAACCAA CACGGCCTTT GGCCAAAAGC AACACCAGTA CTACTGCCGT TAATAGTAAG 
ACAGAAAACT TTATAAAGAA AATTGCTGAA TCCACAACGA CGAGCCAGCA GCTTCCAGAG 
TATACCGAAT CGTCGTCGTC GTCGACAACA ACATTCCCAC CCACAACAGA GTATTTCGAG 
GACGAAAAGG AGCGTAATGC CGAGGACGAA CTTGATTTTC ACTCCATTAT CTACAAGGAG 
TCACATGTCG AGGACGCCTA CGAAAATGTG CGCGAAGGTC ACGTGGCCGG CTGTGGCATC 
ACGGATGAGG TCTCTCAGTG GATGGAGAAC ATACAAAATT CAGCCGTCGA AGAGTTGCCG 
GAGCCCATGT CAAAGGACTA TCAAAAGCTC CACCGGAAGC AGCTGCACAA AAAGTCCGCC 
CCACAGCAAC AACAGCAGCC CCATCCGCCG AAGAAGTACA TCAGCGGGGA TGAGGACTTC 
AAGTATCCCC ACCAGAAGTA CACGAAGGAA GCTAACTTCG CCGAGGGTGC ATTCTACGAT 
CCATCGACCG GACGTCGCCT GGGCTCATCC GCCAACGTGG CCGACTGGCA TCAGCTCGTC 
CACGAGCGCG TCCGCCGCGC CACCGACAAT GGTGCTGGGG ATAGGGGCTC ATCCGGTGGA 
TCTGGACGCG GTCGCGAGGA CAACAAGAAT ACCTGCTCGC TCTACATTCA AACGGATCCA 
TTGATATGGC GCCACATACG CGAAGGCATT GCTGACCACG ATCGTGGACG CAAGTACGAG 
GTGGATGAGA AAACGCGCGA GGAAATCACA TCGTTGATTG CACATCACGT GACGGCCGTT 
AATTACATTT ACCGCAACAC AAAGTTCGAC GGACGCACCG AGCATCGCAA CATACGCTTT 
GAGGTGCAAC GCATTAAGAT CGATGACGAT TCGGCCTGTC GCAATTCCTA CAATGGTCCA 
CACAATGCCT TTTGCAATGA ACACATGGAT GTCTCGAACT TTTTGAATCT GCATTCCCTA 
GAAGATCACT CGGACTTTTG TTTGGCTTAC GTGTTCACCT ACAGAGATTT CACTGGCGGC 
ACTTTGGGTC TGGCCTGGGT GGCCAGTGCG TCGGGAGCCT CTGGTGGAAT TTGCGAGAAG 
TACAAGACGT ACACGGAAAC GGTGGGTGGA CAGTACCAGA GCACCAAGCG ATCACTCAAC 
ACGGGCATCA TCACCTTTGT CAACTACAAC AGCCGGGTGC CGCCGAAAGT GTCGCAGCTT 
ACGTTGGCAC ACGAGATTGG CCACAACTTT GGATCACCTC ACGATTACCC GCAGGAATGT 
CGTCCTGGTG GCCTAAATGG CAATTACATT ATGTTCGCCA GTGCCACCTC CGGTGATAGG 
CCAAATAACT CCAAGTTCTC GCCCTGCTCC ATTCGGAACA TCTCCAATGT CCTTGACGTG 
CTGGTGGGCA ACACGAAGCG CGACTGCTTC AAGGCCTCGG AAGGTGCCTT CTGCGGCAAC 
AAGATCGTGG AGTCTGGCGA GGAATGCGAC TGTGGCTTCA ACGAGGAGGA GTGCAAGGAC 
AAGTGCTGCT ACCCGCGTCT GATCAGCGAG TACGACCAGT CGCTGAACTC CAGTGCCAAG 
GGATGCACGC GCCGCGCCAA GACCCAGTGC TCACCATCGC AGGGTCCGTG CTGTCTGTCC 
AACTCCTGCA CCTTTGTGCC GACGAGCTAC CACCAGAAGT GCAAGGAGGA GACGGAGTGC 
AGCTGGTCGA GCACATGCAA CGGAACCACG GCCGAGTGTC CGGAGCCACG TCATCGCGAT 
GACAAGACCA TGTGCAACAA TGGAACAGCG CTATGCATCC GCGGTGAATG TAGTGGATCG 
CCATGTTTGC TCTGGAATAT GACAAAGTGC TTCCTTACCT CGACCACACT GCCGCACGTG 
AGCAAGCGCA AGTTGTGCGA CTTGGCCTGC CAGGATGGCA ATGACACCTC CACCTGCCGC 
AGCACCAGCG AGTTTGCCGA TAAATATAAT ATTCAAAAGG GTGGTATTAG TCTGCAGCCC 
GGTTCGCCAT GCGATAATTT CCAGGGCTAC TGCGATGTGT TCCTTAAGTG TCGAGCCGTG 
GATGCCGATG GTCCGCTTCT TCGGCTGAAG AATTTGTTGC TCAACCGGAA GACCCTGCAA 
ACGGTGGCCG AGTGGATCGT CGACAATTGG TACCTAGTGG TTCTGATGGG AGTGGCCTTT 
ATTGTGGTCA TGGGTTCGTT CATCAAATGT TGTGCCGTGC ACACGCCCAG TTCCAATCCG 
AAGAAGCGAC GAGCTCGTCG AATCAGCGAA ACTCTAAGAG CACCCATGAA CACGTTGCGT 
AGAATGCAAC GTCATCCCAA TCAGCGAGGA GCAGGTCCTC GAAGCATCCC ACCGCCGGCG 
CATGAGGCGC AGCATTATTC ACGCGGCGGA GATGGTCGCG GCGGCGGCGG TGGAGGCGGA 
GGTCGCCACG GTGGCTCTAG GTCACACCAT CAACAGCATC CGCACGATTG GGATCGTCAT 
CAGGGTGGCC ACTCAATCGT CCCATTGCCC ACCGGCGGCA GCCATTCAAG TCGCAACTCG 
GCGGCGAATC AAGCGAGAAG AAGCGATGGA CGAGGTCCAC GATCCACCAG CAGTGGGCGG 
CCGCAGGCTA TAGCCAGCGG AAGCGGTGCC GCGAGCGGAG CAGCGCGATC TCATGGCGGG 
TACGGAGCCG AACAGGCGAT ACCGGGTTCC ATTGGTGGTG GTGTCCAGGC GGCCATTAGC 
AGCGGCGGTG TGGTGGCTCG GGCCCAGCTG CCGCTGCCAT TGCCGCCGCC AAATGGACAG 
CAGCAAATGC AACAGCAACA ACAACTGCAA CTACAGCAAC CGGCAATTTC GCCGCAGCAG 
CAGCCGCAGC AAGCGTTCTA CACGCCGAAA GGCGTCGCGC CGCCTCCGCT ACAAGTAAGC 
TTTAGTCGGC CGACATCGTT GCACGAGCAA CAGCAGCAGC AACATCATCT GCAGCAGCAG 
CAGCAGCATC AGCAGCAACT GGAGCCGCAG CAGCAGCACG CCTATGCCGA TGCTTATGCG 
GCCTTGGGAC GGGGCCAGTA TGAGTCCACC ACGCGGGCGC CCAACAACAG CAAGGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097160.2
CDS	617..4276
Translation	MSSKCAFNIVFVSIIFIIIVNGYAKDISGVKRGHERLNEYISHYETLNYDHEHIRASHNRARRSVTKDQYVHLKFASHGRDFHLRLKRDLNTFSNKLDFYDSKGPIDVSTDHIYEGEVIGDRNSYVFGSIHNGVFEGKIITERDAYYVEHAKHYFPTNRTATTTPPSTSTTSSATTATKSTQPTRPLAKSNTSTTAVNSKTENFIKKIAESTTTSQQLPEYTESSSSSTTTFPPTTEYFEDEKERNAEDELDFHSIIYKESHVEDAYENVREGHVAGCGITDEVSQWMENIQNSAVEELPEPMSKDYQKLHRKQLHKKSAPQQQQQPHPPKKYISGDEDFKYPHQKYTKEANFAEGAFYDPSTGRRLGSSANVADWHQLVHERVRRATDNGAGDRGSSGGSGRGREDNKNTCSLYIQTDPLIWRHIREGIADHDRGRKYEVDEKTREEITSLIAHHVTAVNYIYRNTKFDGRTEHRNIRFEVQRIKIDDDSACRNSYNGPHNAFCNEHMDVSNFLNLHSLEDHSDFCLAYVFTYRDFTGGTLGLAWVASASGASGGICEKYKTYTETVGGQYQSTKRSLNTGIITFVNYNSRVPPKVSQLTLAHEIGHNFGSPHDYPQECRPGGLNGNYIMFASATSGDRPNNSKFSPCSIRNISNVLDVLVGNTKRDCFKASEGAFCGNKIVESGEECDCGFNEEECKDKCCYPRLISEYDQSLNSSAKGCTRRAKTQCSPSQGPCCLSNSCTFVPTSYHQKCKEETECSWSSTCNGTTAECPEPRHRDDKTMCNNGTALCIRGECSGSPCLLWNMTKCFLTSTTLPHVSKRKLCDLACQDGNDTSTCRSTSEFADKYNIQKGGISLQPGSPCDNFQGYCDVFLKCRAVDADGPLLRLKNLLLNRKTLQTVAEWIVDNWYLVVLMGVAFIVVMGSFIKCCAVHTPSSNPKKRRARRISETLRAPMNTLRRMQRHPNQRGAGPRSIPPPAHEAQHYSRGGDGRGGGGGGGGRHGGSRSHHQQHPHDWDRHQGGHSIVPLPTGGSHSSRNSAANQARRSDGRGPRSTSSGRPQAIASGSGAASGAARSHGGYGAEQAIPGSIGGGVQAAISSGGVVARAQLPLPLPPPNGQQQMQQQQQLQLQQPAISPQQQPQQAFYTPKGVAPPPLQVSFSRPTSLHEQQQQQHHLQQQQQHQQQLEPQQQHAYADAYAALGRGQYESTTRAPNNSKV

Target ORF information:

RefSeq Version	NM_001103690.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster kuzbanian (kuz), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103690.3

ORF Insert Sequence:

ATGTCATCAA AATGTGCTTT CAACATTGTA TTCGTATCGA TCATTTTCAT CATCATCGTA 
AATGGTTACG CAAAAGATAT TTCTGGAGTT AAAAGAGGTC ATGAACGACT TAACGAATAC 
ATATCCCACT ATGAAACACT CAACTATGAT CACGAGCACA TCCGAGCTAG TCACAATAGA 
GCGCGACGAT CAGTGACCAA AGATCAATAT GTACATTTAA AGTTTGCATC ACATGGAAGA 
GACTTCCATC TTAGATTAAA ACGTGATTTA AATACATTTA GCAATAAGTT AGACTTTTAT 
GATAGCAAAG GTCCCATTGA TGTCTCCACG GATCATATCT ATGAGGGCGA AGTGATAGGG 
GATCGTAATA GTTATGTATT TGGTTCCATA CACAATGGGG TATTCGAGGG TAAAATTATA 
ACGGAACGTG ATGCCTATTA TGTTGAACAT GCCAAACATT ATTTTCCCAC AAATCGCACG 
GCGACAACAA CACCACCATC GACTTCAACG ACATCCTCAG CAACAACAGC CACAAAAAGC 
ACACAACCAA CACGGCCTTT GGCCAAAAGC AACACCAGTA CTACTGCCGT TAATAGTAAG 
ACAGAAAACT TTATAAAGAA AATTGCTGAA TCCACAACGA CGAGCCAGCA GCTTCCAGAG 
TATACCGAAT CGTCGTCGTC GTCGACAACA ACATTCCCAC CCACAACAGA GTATTTCGAG 
GACGAAAAGG AGCGTAATGC CGAGGACGAA CTTGATTTTC ACTCCATTAT CTACAAGGAG 
TCACATGTCG AGGACGCCTA CGAAAATGTG CGCGAAGGTC ACGTGGCCGG CTGTGGCATC 
ACGGATGAGG TCTCTCAGTG GATGGAGAAC ATACAAAATT CAGCCGTCGA AGAGTTGCCG 
GAGCCCATGT CAAAGGACTA TCAAAAGCTC CACCGGAAGC AGCTGCACAA AAAGTCCGCC 
CCACAGCAAC AACAGCAGCC CCATCCGCCG AAGAAGTACA TCAGCGGGGA TGAGGACTTC 
AAGTATCCCC ACCAGAAGTA CACGAAGGAA GCTAACTTCG CCGAGGGTGC ATTCTACGAT 
CCATCGACCG GACGTCGCCT GGGCTCATCC GCCAACGTGG CCGACTGGCA TCAGCTCGTC 
CACGAGCGCG TCCGCCGCGC CACCGACAAT GGTGCTGGGG ATAGGGGCTC ATCCGGTGGA 
TCTGGACGCG GTCGCGAGGA CAACAAGAAT ACCTGCTCGC TCTACATTCA AACGGATCCA 
TTGATATGGC GCCACATACG CGAAGGCATT GCTGACCACG ATCGTGGACG CAAGTACGAG 
GTGGATGAGA AAACGCGCGA GGAAATCACA TCGTTGATTG CACATCACGT GACGGCCGTT 
AATTACATTT ACCGCAACAC AAAGTTCGAC GGACGCACCG AGCATCGCAA CATACGCTTT 
GAGGTGCAAC GCATTAAGAT CGATGACGAT TCGGCCTGTC GCAATTCCTA CAATGGTCCA 
CACAATGCCT TTTGCAATGA ACACATGGAT GTCTCGAACT TTTTGAATCT GCATTCCCTA 
GAAGATCACT CGGACTTTTG TTTGGCTTAC GTGTTCACCT ACAGAGATTT CACTGGCGGC 
ACTTTGGGTC TGGCCTGGGT GGCCAGTGCG TCGGGAGCCT CTGGTGGAAT TTGCGAGAAG 
TACAAGACGT ACACGGAAAC GGTGGGTGGA CAGTACCAGA GCACCAAGCG ATCACTCAAC 
ACGGGCATCA TCACCTTTGT CAACTACAAC AGCCGGGTGC CGCCGAAAGT GTCGCAGCTT 
ACGTTGGCAC ACGAGATTGG CCACAACTTT GGATCACCTC ACGATTACCC GCAGGAATGT 
CGTCCTGGTG GCCTAAATGG CAATTACATT ATGTTCGCCA GTGCCACCTC CGGTGATAGG 
CCAAATAACT CCAAGTTCTC GCCCTGCTCC ATTCGGAACA TCTCCAATGT CCTTGACGTG 
CTGGTGGGCA ACACGAAGCG CGACTGCTTC AAGGCCTCGG AAGGTGCCTT CTGCGGCAAC 
AAGATCGTGG AGTCTGGCGA GGAATGCGAC TGTGGCTTCA ACGAGGAGGA GTGCAAGGAC 
AAGTGCTGCT ACCCGCGTCT GATCAGCGAG TACGACCAGT CGCTGAACTC CAGTGCCAAG 
GGATGCACGC GCCGCGCCAA GACCCAGTGC TCACCATCGC AGGGTCCGTG CTGTCTGTCC 
AACTCCTGCA CCTTTGTGCC GACGAGCTAC CACCAGAAGT GCAAGGAGGA GACGGAGTGC 
AGCTGGTCGA GCACATGCAA CGGAACCACG GCCGAGTGTC CGGAGCCACG TCATCGCGAT 
GACAAGACCA TGTGCAACAA TGGAACAGCG CTATGCATCC GCGGTGAATG TAGTGGATCG 
CCATGTTTGC TCTGGAATAT GACAAAGTGC TTCCTTACCT CGACCACACT GCCGCACGTG 
AGCAAGCGCA AGTTGTGCGA CTTGGCCTGC CAGGATGGCA ATGACACCTC CACCTGCCGC 
AGCACCAGCG AGTTTGCCGA TAAATATAAT ATTCAAAAGG GTGGTATTAG TCTGCAGCCC 
GGTTCGCCAT GCGATAATTT CCAGGGCTAC TGCGATGTGT TCCTTAAGTG TCGAGCCGTG 
GATGCCGATG GTCCGCTTCT TCGGCTGAAG AATTTGTTGC TCAACCGGAA GACCCTGCAA 
ACGGTGGCCG AGTGGATCGT CGACAATTGG TACCTAGTGG TTCTGATGGG AGTGGCCTTT 
ATTGTGGTCA TGGGTTCGTT CATCAAATGT TGTGCCGTGC ACACGCCCAG TTCCAATCCG 
AAGAAGCGAC GAGCTCGTCG AATCAGCGAA ACTCTAAGAG CACCCATGAA CACGTTGCGT 
AGAATGCAAC GTCATCCCAA TCAGCGAGGA GCAGGTCCTC GAAGCATCCC ACCGCCGGCG 
CATGAGGCGC AGCATTATTC ACGCGGCGGA GATGGTCGCG GCGGCGGCGG TGGAGGCGGA 
GGTCGCCACG GTGGCTCTAG GTCACACCAT CAACAGCATC CGCACGATTG GGATCGTCAT 
CAGGGTGGCC ACTCAATCGT CCCATTGCCC ACCGGCGGCA GCCATTCAAG TCGCAACTCG 
GCGGCGAATC AAGCGAGAAG AAGCGATGGA CGAGGTCCAC GATCCACCAG CAGTGGGCGG 
CCGCAGGCTA TAGCCAGCGG AAGCGGTGCC GCGAGCGGAG CAGCGCGATC TCATGGCGGG 
TACGGAGCCG AACAGGCGAT ACCGGGTTCC ATTGGTGGTG GTGTCCAGGC GGCCATTAGC 
AGCGGCGGTG TGGTGGCTCG GGCCCAGCTG CCGCTGCCAT TGCCGCCGCC AAATGGACAG 
CAGCAAATGC AACAGCAACA ACAACTGCAA CTACAGCAAC CGGCAATTTC GCCGCAGCAG 
CAGCCGCAGC AAGCGTTCTA CACGCCGAAA GGCGTCGCGC CGCCTCCGCT ACAAGTAAGC 
TTTAGTCGGC CGACATCGTT GCACGAGCAA CAGCAGCAGC AACATCATCT GCAGCAGCAG 
CAGCAGCATC AGCAGCAACT GGAGCCGCAG CAGCAGCACG CCTATGCCGA TGCTTATGCG 
GCCTTGGGAC GGGGCCAGTA TGAGTCCACC ACGCGGGCGC CCAACAACAG CAAGGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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kuz cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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