Gyc32E cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gyc32E
Entrez Gene ID	34553
Full Name	Guanyl cyclase at 32E
Synonyms	CG33114,CG6275,Cyg1,DMRGC,Dmel\CG33114,GC,GYC 32E,Gyc-r
General protein information	Preferred Names Guanyl cyclase at 32E Names CG33114 gene product from transcript CG33114-RG CG33114-PB CG33114-PD CG33114-PG Gyc32E-PB Gyc32E-PD Gyc32E-PG guanyl cyclase at 32E guanylate cyclase receptor-type
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 32D5-32D5

mRNA and Protein(s)

mRNA	Protein	Name
NM_001103678.2	NP_001097148.1	guanylyl cyclase at 32E, isoform B
NM_001298905.1	NP_001285834.1	guanylyl cyclase at 32E, isoform G
NM_176013.2	NP_788027.1	guanylyl cyclase at 32E, isoform D

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Gyc32E	NP_001097148.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008848	XP_319590.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Larvae with mutations in the gene dgcalpha1, which encodes a soluble guanylyl cyclase subunit, have increases in foraging locomotion.
Title: Drosophila soluble guanylyl cyclase mutants exhibit increased foraging locomotion: behavioral and genomic investigations.

The following Gyc32E gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gyc32E cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa16666	NM_001103678.2 Latest version!	Drosophila melanogaster guanylyl cyclase at 32E, transcript variant B (Gyc32E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa21627	NM_001298905.1 Latest version!	Drosophila melanogaster guanylyl cyclase at 32E, transcript variant G (Gyc32E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa21628	NM_176013.2 Latest version!	Drosophila melanogaster guanylyl cyclase at 32E, transcript variant D (Gyc32E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gyc32E ( NM_001103678.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097148.1 Drosophila melanogaster guanylyl cyclase at 32E, transcript variant B (Gyc32E), mRNA.

Price & Availability↑

CloneID	OFa16666
Clone ID Related Accession (Same CDS sequence)	NM_001103678.2
Accession Version	NM_001103678.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	guanylyl cyclase at 32E, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001103678.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCAGGTC CTTGCGCCTC CGCCGCTGCA TTCTCCTGCA TTCTCGTGCT CCTTCTGCTC 
GGCTGTCAGC GGAGCAATCC TCTGGCCGCC GGAGCCACCG TCTCCTCCAT GCGTCGACTG 
ACGGATACGA TAAATATTGG ATTCCTGGCT GAATATTCTC AGATGCGAGT GACACTCGGC 
GGACTGCCAC TTGCCATCGA GGATGTGAAC AAGAATCCCA ACCTATTGCC GGGCAAGAAG 
TTGGCTTTCA AACCGGTTGA CATTGGTCAC AAGATGAGCG CCTATCGCGT TAAACCGTTG 
AGGGCGATGA CACAGATGCG GGAAGCTGGC GTGACCGCAT TCATTGGTCC CGACGAGAGC 
TGCACCACGG AGGCGCTGCT GGCCTCCGCA TGGAACACAC CAATGCTATC CTTCAAATGC 
TCCGATCCAA TTGTCTCAAA CAAAAGTACA TTTCACACCT TTGCTCGAAC TTTAGCGCCG 
GCTTCCAAGG TCTCGAAAAG TGTTATTAGT CTGCTGAACG CCTTCCATTG GAATAAATTC 
TCCATAGTAG TCAGCTCGAA ACCGATTTGG GGCTCGGATG TAGCCCGTGC CATTCAGGAA 
TTGGCGGAAG CCAGGAACTT TACCATCAGC CACTTTAAAT ATATTTCGGA CTACATACCA 
ACCACAAAGA CCCTTTCGCA GATCGATAAA ATTATTGAGG AAACCTATGC CACAACTCGC 
ATCTATGTCT TCATTGGTGA GCACATTGCC ATGGTGGACT TTGTGCGAGG ACTCCAGAAT 
CGTCGGCTGC TCGAGAGTGG GGACTATATC GTGGTTTCTG TGGACGACGA GATCTACGAC 
TCCAATCGAA GGGTGAACAT TATGGAACGT AATTATTTAG ATCCTTATAT TAGAAAAGAA 
AAGAGCAAAT CGCTGGACAA AATCTCATTT CGTTCAGTTA TAAAAATAAG CATGACATAT 
CCACAAAACC CCCATATTCG CGACATTTGC TCGAAAATAA AAGACTATGC GCGAAAAACT 
CCATTTCTTG TGCCATATCA TCAACGTGTC TTTGACAACA TATCGGTTCC CATATATGGC 
CTACATCTTT ACGACAGTGT TATGATCTAT GTTCGAGCTA TAACTGAAGT TTTAAGACTG 
GGCGGTGATA TTTACGATGG AAACTTGGTA ATGAGTCACA TCTTTAATAG GTCCTACCAT 
TCCATACAAG GATTTGATGT ATACATAGAC TCTAATGGTG ATGCTGAGGG TAATTACACA 
GTCATCACTC TGCAAAACGA TGTGGGCAGT GGAGCATCGA TAGGTTCTTT AGCCAAGATG 
TCAATGCAAC CAGTTGGGTT CTTTGCCTAC GACAAGAACT CTGTTATTCC AGAATTTCGC 
TACATAAAGA ATGATAGACC CATCCAGTGG CTAAATGGGC GTCCTCCGCT CGCAGAACCC 
CTATGTGGTT TCCATGGCGA ACTATGTCCC CGAAAGAAAC TCGACTGGCG GTACCTGGTA 
TCAGGTCCAC TGTGTGCCCT CGTTGTTGTC GTGGCTATTG CACTGCTTAT CAAGCATTAC 
AGGTATGAAC AGACTCTGGC CGGACTTTTG TGGAAAGTGG ACATGAAGGA TGTCACCGTT 
ATAAATCTTG GCGAATACAA CAATCCCACC AATAAGAATA TTTTCCAAAT CTGCCGTCAA 
AGCATTTTAG TGGTTGGTGA GCCCAACAAG CGGTCCTTTA CAAATATAGC TCTATTTCGT 
GGCAATATTG TGGCCATGAA AAAGATTCAC AAGAAGAGCG TAGACATTAC CCGTTCAATT 
CGCAAGGAAC TAAAATTGAT GCGAGAGGTG CGACATGAGA ATATTATCAA CTTCATTGGA 
GCGTCCACGG ATCACGGATC CGTGATCATC TTCACCACTT ACTGTGCACG TGGCAGTCTG 
GAGGACGTGC TGGCCAACGA AGATTTGCAC CTGGACCACA TGTTCATCTC CTCCCTCGTG 
TCCGATATAC TTAAGGGAAT GATCTACCTG CACGACTCGG AGATTATATC CCATGGCAAT 
TTACGGTCGA GCAACTGCCT TATCGACTCC CGTTGGGTGT GCCAAATCTC GGACTTTGGA 
TTGCACGAGC TGAAAGCTGG CCAAGAAGAG CCCAACAAGA GTGAACTGGA ACTGAAACGT 
GCCCTCTGCA TGGCGCCGGA GCTTTTGAGG GACGCCTACC GACCAGGACG CGGATCCCAA 
AAGGGGGATG TCTATTCCTT TGGCATTTTG CTCTACGAAA TGATTGGCCG CAAAGGACCT 
TGGGGTGACA CGGCCTACTC AAAGGAGGAA ATAATTCAGT TTGTCAAGTG TCCTGAAATG 
CTGCAGCACG GAGTATTCCG ACCTGCATTG ACGCACACTC ACTTGGACAT TCCGGACTAC 
ATCCGTAAAT GCCTGTGCCA GTGCTGGGAT GAAGATCCCG AGGTCAGGCC CGATATCCGG 
CTGGTTCGCA TGCATTTGAA GGAGCTTCAG GCTGGCTTAA AACCCAACAT ATTCGACAAT 
ATGCTATCGA TAATGGAGAA ATATGCTTAC AATCTTGAGG GTTTGGTTCA GGAGCGAACC 
AATTTGTTGT ACGAAGAGAA GAAGAAAACA GATATGCTCC TTTATCAGAT GCTACCAAGA 
CCCGTAGCTG AACTTCTGAA GCGAGGAGAT CCTGTCGAGG CTGAGTGCTT CGACTGTGTC 
ACTATATTGT TCAGTGATAT AGTGGGATTC ACCGAACTCT GCACCACCAG CACTCCCTTT 
GAGGTGGTGG AGATGCTTAA CGATTGGTAC ACCTGCTGCG ACTCCATAAT ATCCAATTAT 
GATGTCTATA AGGTTGAGAC AATTGGCGAT GCCTATATGG TTGTGTCTGG ATTGCCACTG 
CAGAACGGAA GTCGTCATGC TGGAGAGATA GCCTCACTGG CTCTGCATCT CTTAGAGACT 
GTGGGAAATC TGAAGATTAG GCACAAGCCC ACCGAAACAG TTCAATTACG TATCGGAGTT 
CACTCTGGTC CTTGTGCAGC AGGAGTGGTG GGTCAGAAGA TGCCCAGATA CTGCCTATTC 
GGAGACACGG TGAACACAGC AAGTCGGATG GAGAGCACCG GTGACTCGAT GAGAATTCAT 
ATATCCGAAG CCACCTACCA GCTGCTCCAG GTGATCGGTA GCTATGTGTG CATTGAAAGG 
GGTCTGACCA GCATAAAGGG CAAGGGCGAC ATGCGAACCT ATTGGCTGAC CAAACGTCAG 
CAACCCGAGT TAACGCCGGA TCTGATTAGC ACCGTGGATA CTTTGGACAC CTACTGCTCC 
GGGCCACGTG AGAGCATGGA GGTCTCAGTC CACCAATATT GTAGTCCGGC CTCGAATAAC 
TACAGATTGG GATCCTGCAA CTGTGACACC AAGTGTTTAT ATAGCCGTCG TTCGGACGAC 
AATGTAACTA ATTCCCATGG AACATCCGAG TTTCCAAAGG TCAGTGAACC CGCGCAGGTC 
AACTGCAATC AATTATGTGT TTGCCGATTG AACAGCAGCC AAATGTTTAA TAATCGAGGT 
CCTCGATCAG CGCCTAGTAT CACATTTAGA CTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097148.1
CDS	227..3802
Translation	MPGPCASAAAFSCILVLLLLGCQRSNPLAAGATVSSMRRLTDTINIGFLAEYSQMRVTLGGLPLAIEDVNKNPNLLPGKKLAFKPVDIGHKMSAYRVKPLRAMTQMREAGVTAFIGPDESCTTEALLASAWNTPMLSFKCSDPIVSNKSTFHTFARTLAPASKVSKSVISLLNAFHWNKFSIVVSSKPIWGSDVARAIQELAEARNFTISHFKYISDYIPTTKTLSQIDKIIEETYATTRIYVFIGEHIAMVDFVRGLQNRRLLESGDYIVVSVDDEIYDSNRRVNIMERNYLDPYIRKEKSKSLDKISFRSVIKISMTYPQNPHIRDICSKIKDYARKTPFLVPYHQRVFDNISVPIYGLHLYDSVMIYVRAITEVLRLGGDIYDGNLVMSHIFNRSYHSIQGFDVYIDSNGDAEGNYTVITLQNDVGSGASIGSLAKMSMQPVGFFAYDKNSVIPEFRYIKNDRPIQWLNGRPPLAEPLCGFHGELCPRKKLDWRYLVSGPLCALVVVVAIALLIKHYRYEQTLAGLLWKVDMKDVTVINLGEYNNPTNKNIFQICRQSILVVGEPNKRSFTNIALFRGNIVAMKKIHKKSVDITRSIRKELKLMREVRHENIINFIGASTDHGSVIIFTTYCARGSLEDVLANEDLHLDHMFISSLVSDILKGMIYLHDSEIISHGNLRSSNCLIDSRWVCQISDFGLHELKAGQEEPNKSELELKRALCMAPELLRDAYRPGRGSQKGDVYSFGILLYEMIGRKGPWGDTAYSKEEIIQFVKCPEMLQHGVFRPALTHTHLDIPDYIRKCLCQCWDEDPEVRPDIRLVRMHLKELQAGLKPNIFDNMLSIMEKYAYNLEGLVQERTNLLYEEKKKTDMLLYQMLPRPVAELLKRGDPVEAECFDCVTILFSDIVGFTELCTTSTPFEVVEMLNDWYTCCDSIISNYDVYKVETIGDAYMVVSGLPLQNGSRHAGEIASLALHLLETVGNLKIRHKPTETVQLRIGVHSGPCAAGVVGQKMPRYCLFGDTVNTASRMESTGDSMRIHISEATYQLLQVIGSYVCIERGLTSIKGKGDMRTYWLTKRQQPELTPDLISTVDTLDTYCSGPRESMEVSVHQYCSPASNNYRLGSCNCDTKCLYSRRSDDNVTNSHGTSEFPKVSEPAQVNCNQLCVCRLNSSQMFNNRGPRSAPSITFRL

Target ORF information:

RefSeq Version	NM_001103678.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster guanylyl cyclase at 32E, transcript variant B (Gyc32E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103678.2

ORF Insert Sequence:

ATGCCAGGTC CTTGCGCCTC CGCCGCTGCA TTCTCCTGCA TTCTCGTGCT CCTTCTGCTC 
GGCTGTCAGC GGAGCAATCC TCTGGCCGCC GGAGCCACCG TCTCCTCCAT GCGTCGACTG 
ACGGATACGA TAAATATTGG ATTCCTGGCT GAATATTCTC AGATGCGAGT GACACTCGGC 
GGACTGCCAC TTGCCATCGA GGATGTGAAC AAGAATCCCA ACCTATTGCC GGGCAAGAAG 
TTGGCTTTCA AACCGGTTGA CATTGGTCAC AAGATGAGCG CCTATCGCGT TAAACCGTTG 
AGGGCGATGA CACAGATGCG GGAAGCTGGC GTGACCGCAT TCATTGGTCC CGACGAGAGC 
TGCACCACGG AGGCGCTGCT GGCCTCCGCA TGGAACACAC CAATGCTATC CTTCAAATGC 
TCCGATCCAA TTGTCTCAAA CAAAAGTACA TTTCACACCT TTGCTCGAAC TTTAGCGCCG 
GCTTCCAAGG TCTCGAAAAG TGTTATTAGT CTGCTGAACG CCTTCCATTG GAATAAATTC 
TCCATAGTAG TCAGCTCGAA ACCGATTTGG GGCTCGGATG TAGCCCGTGC CATTCAGGAA 
TTGGCGGAAG CCAGGAACTT TACCATCAGC CACTTTAAAT ATATTTCGGA CTACATACCA 
ACCACAAAGA CCCTTTCGCA GATCGATAAA ATTATTGAGG AAACCTATGC CACAACTCGC 
ATCTATGTCT TCATTGGTGA GCACATTGCC ATGGTGGACT TTGTGCGAGG ACTCCAGAAT 
CGTCGGCTGC TCGAGAGTGG GGACTATATC GTGGTTTCTG TGGACGACGA GATCTACGAC 
TCCAATCGAA GGGTGAACAT TATGGAACGT AATTATTTAG ATCCTTATAT TAGAAAAGAA 
AAGAGCAAAT CGCTGGACAA AATCTCATTT CGTTCAGTTA TAAAAATAAG CATGACATAT 
CCACAAAACC CCCATATTCG CGACATTTGC TCGAAAATAA AAGACTATGC GCGAAAAACT 
CCATTTCTTG TGCCATATCA TCAACGTGTC TTTGACAACA TATCGGTTCC CATATATGGC 
CTACATCTTT ACGACAGTGT TATGATCTAT GTTCGAGCTA TAACTGAAGT TTTAAGACTG 
GGCGGTGATA TTTACGATGG AAACTTGGTA ATGAGTCACA TCTTTAATAG GTCCTACCAT 
TCCATACAAG GATTTGATGT ATACATAGAC TCTAATGGTG ATGCTGAGGG TAATTACACA 
GTCATCACTC TGCAAAACGA TGTGGGCAGT GGAGCATCGA TAGGTTCTTT AGCCAAGATG 
TCAATGCAAC CAGTTGGGTT CTTTGCCTAC GACAAGAACT CTGTTATTCC AGAATTTCGC 
TACATAAAGA ATGATAGACC CATCCAGTGG CTAAATGGGC GTCCTCCGCT CGCAGAACCC 
CTATGTGGTT TCCATGGCGA ACTATGTCCC CGAAAGAAAC TCGACTGGCG GTACCTGGTA 
TCAGGTCCAC TGTGTGCCCT CGTTGTTGTC GTGGCTATTG CACTGCTTAT CAAGCATTAC 
AGGTATGAAC AGACTCTGGC CGGACTTTTG TGGAAAGTGG ACATGAAGGA TGTCACCGTT 
ATAAATCTTG GCGAATACAA CAATCCCACC AATAAGAATA TTTTCCAAAT CTGCCGTCAA 
AGCATTTTAG TGGTTGGTGA GCCCAACAAG CGGTCCTTTA CAAATATAGC TCTATTTCGT 
GGCAATATTG TGGCCATGAA AAAGATTCAC AAGAAGAGCG TAGACATTAC CCGTTCAATT 
CGCAAGGAAC TAAAATTGAT GCGAGAGGTG CGACATGAGA ATATTATCAA CTTCATTGGA 
GCGTCCACGG ATCACGGATC CGTGATCATC TTCACCACTT ACTGTGCACG TGGCAGTCTG 
GAGGACGTGC TGGCCAACGA AGATTTGCAC CTGGACCACA TGTTCATCTC CTCCCTCGTG 
TCCGATATAC TTAAGGGAAT GATCTACCTG CACGACTCGG AGATTATATC CCATGGCAAT 
TTACGGTCGA GCAACTGCCT TATCGACTCC CGTTGGGTGT GCCAAATCTC GGACTTTGGA 
TTGCACGAGC TGAAAGCTGG CCAAGAAGAG CCCAACAAGA GTGAACTGGA ACTGAAACGT 
GCCCTCTGCA TGGCGCCGGA GCTTTTGAGG GACGCCTACC GACCAGGACG CGGATCCCAA 
AAGGGGGATG TCTATTCCTT TGGCATTTTG CTCTACGAAA TGATTGGCCG CAAAGGACCT 
TGGGGTGACA CGGCCTACTC AAAGGAGGAA ATAATTCAGT TTGTCAAGTG TCCTGAAATG 
CTGCAGCACG GAGTATTCCG ACCTGCATTG ACGCACACTC ACTTGGACAT TCCGGACTAC 
ATCCGTAAAT GCCTGTGCCA GTGCTGGGAT GAAGATCCCG AGGTCAGGCC CGATATCCGG 
CTGGTTCGCA TGCATTTGAA GGAGCTTCAG GCTGGCTTAA AACCCAACAT ATTCGACAAT 
ATGCTATCGA TAATGGAGAA ATATGCTTAC AATCTTGAGG GTTTGGTTCA GGAGCGAACC 
AATTTGTTGT ACGAAGAGAA GAAGAAAACA GATATGCTCC TTTATCAGAT GCTACCAAGA 
CCCGTAGCTG AACTTCTGAA GCGAGGAGAT CCTGTCGAGG CTGAGTGCTT CGACTGTGTC 
ACTATATTGT TCAGTGATAT AGTGGGATTC ACCGAACTCT GCACCACCAG CACTCCCTTT 
GAGGTGGTGG AGATGCTTAA CGATTGGTAC ACCTGCTGCG ACTCCATAAT ATCCAATTAT 
GATGTCTATA AGGTTGAGAC AATTGGCGAT GCCTATATGG TTGTGTCTGG ATTGCCACTG 
CAGAACGGAA GTCGTCATGC TGGAGAGATA GCCTCACTGG CTCTGCATCT CTTAGAGACT 
GTGGGAAATC TGAAGATTAG GCACAAGCCC ACCGAAACAG TTCAATTACG TATCGGAGTT 
CACTCTGGTC CTTGTGCAGC AGGAGTGGTG GGTCAGAAGA TGCCCAGATA CTGCCTATTC 
GGAGACACGG TGAACACAGC AAGTCGGATG GAGAGCACCG GTGACTCGAT GAGAATTCAT 
ATATCCGAAG CCACCTACCA GCTGCTCCAG GTGATCGGTA GCTATGTGTG CATTGAAAGG 
GGTCTGACCA GCATAAAGGG CAAGGGCGAC ATGCGAACCT ATTGGCTGAC CAAACGTCAG 
CAACCCGAGT TAACGCCGGA TCTGATTAGC ACCGTGGATA CTTTGGACAC CTACTGCTCC 
GGGCCACGTG AGAGCATGGA GGTCTCAGTC CACCAATATT GTAGTCCGGC CTCGAATAAC 
TACAGATTGG GATCCTGCAA CTGTGACACC AAGTGTTTAT ATAGCCGTCG TTCGGACGAC 
AATGTAACTA ATTCCCATGG AACATCCGAG TTTCCAAAGG TCAGTGAACC CGCGCAGGTC 
AACTGCAATC AATTATGTGT TTGCCGATTG AACAGCAGCC AAATGTTTAA TAATCGAGGT 
CCTCGATCAG CGCCTAGTAT CACATTTAGA CTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gyc32E ( NM_001298905.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285834.1 Drosophila melanogaster guanylyl cyclase at 32E, transcript variant G (Gyc32E), mRNA.

Price & Availability↑

CloneID	OFa21627
Clone ID Related Accession (Same CDS sequence)	NM_001298905.1
Accession Version	NM_001298905.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	guanylyl cyclase at 32E, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCAGGTC CTTGCGCCTC CGCCGCTGCA TTCTCCTGCA TTCTCGTGCT CCTTCTGCTC 
GGCTGTCAGC GGAGCAATCC TCTGGCCGCC GGAGCCACCG TCTCCTCCAT GCGTCGACTG 
ACGGATACGA TAAATATTGG ATTCCTGGCT GAATATTCTC AGATGCGAGT GACACTCGGC 
GGACTGCCAC TTGCCATCGA GGATGTGAAC AAGAATCCCA ACCTATTGCC GGGCAAGAAG 
TTGGCTTTCA AACCGGTTGA CATTGGTCAC AAGATGAGCG CCTATCGCGT TAAACCGTTG 
AGGGCGATGA CACAGATGCG GGAAGCTGGC GTGACCGCAT TCATTGGTCC CGACGAGAGC 
TGCACCACGG AGGCGCTGCT GGCCTCCGCA TGGAACACAC CAATGCTATC CTTCAAATGC 
TCCGATCCAA TTGTCTCAAA CAAAAGTACA TTTCACACCT TTGCTCGAAC TTTAGCGCCG 
GCTTCCAAGG TCTCGAAAAG TGTTATTAGT CTGCTGAACG CCTTCCATTG GAATAAATTC 
TCCATAGTAG TCAGCTCGAA ACCGATTTGG GGCTCGGATG TAGCCCGTGC CATTCAGGAA 
TTGGCGGAAG CCAGGAACTT TACCATCAGC CACTTTAAAT ATATTTCGGA CTACATACCA 
ACCACAAAGA CCCTTTCGCA GATCGATAAA ATTATTGAGG AAACCTATGC CACAACTCGC 
ATCTATGTCT TCATTGGTGA GCACATTGCC ATGGTGGACT TTGTGCGAGG ACTCCAGAAT 
CGTCGGCTGC TCGAGAGTGG GGACTATATC GTGGTTTCTG TGGACGACGA GATCTACGAC 
TCCAATCGAA GGGTGAACAT TATGGAACGT ATCGACATTT GCTCGAAAAT AAAAGACTAT 
GCGCGAAAAA CTCCATTTCT TGTGCCATAT CATCAACGTG TCTTTGACAA CATATCGGTT 
CCCATATATG GCCTACATCT TTACGACAGT GTTATGATCT ATGTTCGAGC TATAACTGAA 
GTTTTAAGAC TGGGCGGTGA TATTTACGAT GGAAACTTGG TAATGAGTCA CATCTTTAAT 
AGGTCCTACC ATTCCATACA AGGATTTGAT GTATACATAG ACTCTAATGG TGATGCTGAG 
GGTAATTACA CAGTCATCAC TCTGCAAAAC GATGTGGGCA GTGGAGCATC GATAGGTTCT 
TTAGCCAAGA TGTCAATGCA ACCAGTTGGG TTCTTTGCCT ACGACAAGAA CTCTGTTATT 
CCAGAATTTC GCTACATAAA GAATGATAGA CCCATCCAGT GGCTAAATGG GCGTCCTCCG 
CTCGCAGAAC CCCTATGTGG TTTCCATGGC GAACTATGTC CCCGAAAGAA ACTCGACTGG 
CGGTACCTGG TATCAGGTCC ACTGTGTGCC CTCGTTGTTG TCGTGGCTAT TGCACTGCTT 
ATCAAGCATT ACAGGTATGA ACAGACTCTG GCCGGACTTT TGTGGAAAGT GGACATGAAG 
GATGTCACCG TTATAAATCT TGGCGAATAC AACAATCCCA CCAATAAGAA TATTTTCCAA 
ATCTGCCGTC AAAGCATTTT AGTGGTTGGT GAGCCCAACA AGCGGTCCTT TACAAATATA 
GCTCTATTTC GTGGCAATAT TGTGGCCATG AAAAAGATTC ACAAGAAGAG CGTAGACATT 
ACCCGTTCAA TTCGCAAGGA ACTAAAATTG ATGCGAGAGG TGCGACATGA GAATATTATC 
AACTTCATTG GAGCGTCCAC GGATCACGGA TCCGTGATCA TCTTCACCAC TTACTGTGCA 
CGTGGCAGTC TGGAGGACGT GCTGGCCAAC GAAGATTTGC ACCTGGACCA CATGTTCATC 
TCCTCCCTCG TGTCCGATAT ACTTAAGGGA ATGATCTACC TGCACGACTC GGAGATTATA 
TCCCATGGCA ATTTACGGTC GAGCAACTGC CTTATCGACT CCCGTTGGGT GTGCCAAATC 
TCGGACTTTG GATTGCACGA GCTGAAAGCT GGCCAAGAAG AGCCCAACAA GAGTGAACTG 
GAACTGAAAC GTGCCCTCTG CATGGCGCCG GAGCTTTTGA GGGACGCCTA CCGACCAGGA 
CGCGGATCCC AAAAGGGGGA TGTCTATTCC TTTGGCATTT TGCTCTACGA AATGATTGGC 
CGCAAAGGAC CTTGGGGTGA CACGGCCTAC TCAAAGGAGG AAATAATTCA GTTTGTCAAG 
TGTCCTGAAA TGCTGCAGCA CGGAGTATTC CGACCTGCAT TGACGCACAC TCACTTGGAC 
ATTCCGGACT ACATCCGTAA ATGCCTGTGC CAGTGCTGGG ATGAAGATCC CGAGGTCAGG 
CCCGATATCC GGCTGGTTCG CATGCATTTG AAGGAGCTTC AGGCTGGCTT AAAACCCAAC 
ATATTCGACA ATATGCTATC GATAATGGAG AAATATGCTT ACAATCTTGA GGGTTTGGTT 
CAGGAGCGAA CCAATTTGTT GTACGAAGAG AAGAAGAAAA CAGATATGCT CCTTTATCAG 
ATGCTACCAA GACCCGTAGC TGAACTTCTG AAGCGAGGAG ATCCTGTCGA GGCTGAGTGC 
TTCGACTGTG TCACTATATT GTTCAGTGAT ATAGTGGGAT TCACCGAACT CTGCACCACC 
AGCACTCCCT TTGAGGTGGT GGAGATGCTT AACGATTGGT ACACCTGCTG CGACTCCATA 
ATATCCAATT ATGATGTCTA TAAGGTTGAG ACAATTGGCG ATGCCTATAT GGTTGTGTCT 
GGATTGCCAC TGCAGAACGG AAGTCGTCAT GCTGGAGAGA TAGCCTCACT GGCTCTGCAT 
CTCTTAGAGA CTGTGGGAAA TCTGAAGATT AGGCACAAGC CCACCGAAAC AGTTCAATTA 
CGTATCGGAG TTCACTCTGG TCCTTGTGCA GCAGGAGTGG TGGGTCAGAA GATGCCCAGA 
TACTGCCTAT TCGGAGACAC GGTGAACACA GCAAGTCGGA TGGAGAGCAC CGGTGACTCG 
ATGAGAATTC ATATATCCGA AGCCACCTAC CAGCTGCTCC AGGTGATCGG TAGCTATGTG 
TGCATTGAAA GGGGTCTGAC CAGCATAAAG GGCAAGGGCG ACATGCGAAC CTATTGGCTG 
ACCAAACGTC AGCAACCCGA GTTAACGCCG GATCTGATTA GCACCGTGGA TACTTTGGAC 
ACCTACTGCT CCGGGCCACG TGAGAGCATG GAGGTCTCAG TCCACCAATA TTGTAGTCCG 
GCCTCGAATA ACTACAGATT GGGATCCTGC AACTGTGACA CCAAGTGTTT ATATAGCCGT 
CGTTCGGACG ACAATGTAAC TAATTCCCAT GGAACATCCG AGTTTCCAAA GGTCAGTGAA 
CCCGCGCAGG TCAACTGCAA TCAATTATGT GTTTGCCGAT TGAACAGCAG CCAAATGTTT 
AATAATCGAG GTCCTCGATC AGCGCCTAGT ATCACATTTA GACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285834.1
CDS	227..3694
Translation	MPGPCASAAAFSCILVLLLLGCQRSNPLAAGATVSSMRRLTDTINIGFLAEYSQMRVTLGGLPLAIEDVNKNPNLLPGKKLAFKPVDIGHKMSAYRVKPLRAMTQMREAGVTAFIGPDESCTTEALLASAWNTPMLSFKCSDPIVSNKSTFHTFARTLAPASKVSKSVISLLNAFHWNKFSIVVSSKPIWGSDVARAIQELAEARNFTISHFKYISDYIPTTKTLSQIDKIIEETYATTRIYVFIGEHIAMVDFVRGLQNRRLLESGDYIVVSVDDEIYDSNRRVNIMERIDICSKIKDYARKTPFLVPYHQRVFDNISVPIYGLHLYDSVMIYVRAITEVLRLGGDIYDGNLVMSHIFNRSYHSIQGFDVYIDSNGDAEGNYTVITLQNDVGSGASIGSLAKMSMQPVGFFAYDKNSVIPEFRYIKNDRPIQWLNGRPPLAEPLCGFHGELCPRKKLDWRYLVSGPLCALVVVVAIALLIKHYRYEQTLAGLLWKVDMKDVTVINLGEYNNPTNKNIFQICRQSILVVGEPNKRSFTNIALFRGNIVAMKKIHKKSVDITRSIRKELKLMREVRHENIINFIGASTDHGSVIIFTTYCARGSLEDVLANEDLHLDHMFISSLVSDILKGMIYLHDSEIISHGNLRSSNCLIDSRWVCQISDFGLHELKAGQEEPNKSELELKRALCMAPELLRDAYRPGRGSQKGDVYSFGILLYEMIGRKGPWGDTAYSKEEIIQFVKCPEMLQHGVFRPALTHTHLDIPDYIRKCLCQCWDEDPEVRPDIRLVRMHLKELQAGLKPNIFDNMLSIMEKYAYNLEGLVQERTNLLYEEKKKTDMLLYQMLPRPVAELLKRGDPVEAECFDCVTILFSDIVGFTELCTTSTPFEVVEMLNDWYTCCDSIISNYDVYKVETIGDAYMVVSGLPLQNGSRHAGEIASLALHLLETVGNLKIRHKPTETVQLRIGVHSGPCAAGVVGQKMPRYCLFGDTVNTASRMESTGDSMRIHISEATYQLLQVIGSYVCIERGLTSIKGKGDMRTYWLTKRQQPELTPDLISTVDTLDTYCSGPRESMEVSVHQYCSPASNNYRLGSCNCDTKCLYSRRSDDNVTNSHGTSEFPKVSEPAQVNCNQLCVCRLNSSQMFNNRGPRSAPSITFRL

Target ORF information:

RefSeq Version	NM_001298905.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster guanylyl cyclase at 32E, transcript variant G (Gyc32E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298905.1

ORF Insert Sequence:

ATGCCAGGTC CTTGCGCCTC CGCCGCTGCA TTCTCCTGCA TTCTCGTGCT CCTTCTGCTC 
GGCTGTCAGC GGAGCAATCC TCTGGCCGCC GGAGCCACCG TCTCCTCCAT GCGTCGACTG 
ACGGATACGA TAAATATTGG ATTCCTGGCT GAATATTCTC AGATGCGAGT GACACTCGGC 
GGACTGCCAC TTGCCATCGA GGATGTGAAC AAGAATCCCA ACCTATTGCC GGGCAAGAAG 
TTGGCTTTCA AACCGGTTGA CATTGGTCAC AAGATGAGCG CCTATCGCGT TAAACCGTTG 
AGGGCGATGA CACAGATGCG GGAAGCTGGC GTGACCGCAT TCATTGGTCC CGACGAGAGC 
TGCACCACGG AGGCGCTGCT GGCCTCCGCA TGGAACACAC CAATGCTATC CTTCAAATGC 
TCCGATCCAA TTGTCTCAAA CAAAAGTACA TTTCACACCT TTGCTCGAAC TTTAGCGCCG 
GCTTCCAAGG TCTCGAAAAG TGTTATTAGT CTGCTGAACG CCTTCCATTG GAATAAATTC 
TCCATAGTAG TCAGCTCGAA ACCGATTTGG GGCTCGGATG TAGCCCGTGC CATTCAGGAA 
TTGGCGGAAG CCAGGAACTT TACCATCAGC CACTTTAAAT ATATTTCGGA CTACATACCA 
ACCACAAAGA CCCTTTCGCA GATCGATAAA ATTATTGAGG AAACCTATGC CACAACTCGC 
ATCTATGTCT TCATTGGTGA GCACATTGCC ATGGTGGACT TTGTGCGAGG ACTCCAGAAT 
CGTCGGCTGC TCGAGAGTGG GGACTATATC GTGGTTTCTG TGGACGACGA GATCTACGAC 
TCCAATCGAA GGGTGAACAT TATGGAACGT ATCGACATTT GCTCGAAAAT AAAAGACTAT 
GCGCGAAAAA CTCCATTTCT TGTGCCATAT CATCAACGTG TCTTTGACAA CATATCGGTT 
CCCATATATG GCCTACATCT TTACGACAGT GTTATGATCT ATGTTCGAGC TATAACTGAA 
GTTTTAAGAC TGGGCGGTGA TATTTACGAT GGAAACTTGG TAATGAGTCA CATCTTTAAT 
AGGTCCTACC ATTCCATACA AGGATTTGAT GTATACATAG ACTCTAATGG TGATGCTGAG 
GGTAATTACA CAGTCATCAC TCTGCAAAAC GATGTGGGCA GTGGAGCATC GATAGGTTCT 
TTAGCCAAGA TGTCAATGCA ACCAGTTGGG TTCTTTGCCT ACGACAAGAA CTCTGTTATT 
CCAGAATTTC GCTACATAAA GAATGATAGA CCCATCCAGT GGCTAAATGG GCGTCCTCCG 
CTCGCAGAAC CCCTATGTGG TTTCCATGGC GAACTATGTC CCCGAAAGAA ACTCGACTGG 
CGGTACCTGG TATCAGGTCC ACTGTGTGCC CTCGTTGTTG TCGTGGCTAT TGCACTGCTT 
ATCAAGCATT ACAGGTATGA ACAGACTCTG GCCGGACTTT TGTGGAAAGT GGACATGAAG 
GATGTCACCG TTATAAATCT TGGCGAATAC AACAATCCCA CCAATAAGAA TATTTTCCAA 
ATCTGCCGTC AAAGCATTTT AGTGGTTGGT GAGCCCAACA AGCGGTCCTT TACAAATATA 
GCTCTATTTC GTGGCAATAT TGTGGCCATG AAAAAGATTC ACAAGAAGAG CGTAGACATT 
ACCCGTTCAA TTCGCAAGGA ACTAAAATTG ATGCGAGAGG TGCGACATGA GAATATTATC 
AACTTCATTG GAGCGTCCAC GGATCACGGA TCCGTGATCA TCTTCACCAC TTACTGTGCA 
CGTGGCAGTC TGGAGGACGT GCTGGCCAAC GAAGATTTGC ACCTGGACCA CATGTTCATC 
TCCTCCCTCG TGTCCGATAT ACTTAAGGGA ATGATCTACC TGCACGACTC GGAGATTATA 
TCCCATGGCA ATTTACGGTC GAGCAACTGC CTTATCGACT CCCGTTGGGT GTGCCAAATC 
TCGGACTTTG GATTGCACGA GCTGAAAGCT GGCCAAGAAG AGCCCAACAA GAGTGAACTG 
GAACTGAAAC GTGCCCTCTG CATGGCGCCG GAGCTTTTGA GGGACGCCTA CCGACCAGGA 
CGCGGATCCC AAAAGGGGGA TGTCTATTCC TTTGGCATTT TGCTCTACGA AATGATTGGC 
CGCAAAGGAC CTTGGGGTGA CACGGCCTAC TCAAAGGAGG AAATAATTCA GTTTGTCAAG 
TGTCCTGAAA TGCTGCAGCA CGGAGTATTC CGACCTGCAT TGACGCACAC TCACTTGGAC 
ATTCCGGACT ACATCCGTAA ATGCCTGTGC CAGTGCTGGG ATGAAGATCC CGAGGTCAGG 
CCCGATATCC GGCTGGTTCG CATGCATTTG AAGGAGCTTC AGGCTGGCTT AAAACCCAAC 
ATATTCGACA ATATGCTATC GATAATGGAG AAATATGCTT ACAATCTTGA GGGTTTGGTT 
CAGGAGCGAA CCAATTTGTT GTACGAAGAG AAGAAGAAAA CAGATATGCT CCTTTATCAG 
ATGCTACCAA GACCCGTAGC TGAACTTCTG AAGCGAGGAG ATCCTGTCGA GGCTGAGTGC 
TTCGACTGTG TCACTATATT GTTCAGTGAT ATAGTGGGAT TCACCGAACT CTGCACCACC 
AGCACTCCCT TTGAGGTGGT GGAGATGCTT AACGATTGGT ACACCTGCTG CGACTCCATA 
ATATCCAATT ATGATGTCTA TAAGGTTGAG ACAATTGGCG ATGCCTATAT GGTTGTGTCT 
GGATTGCCAC TGCAGAACGG AAGTCGTCAT GCTGGAGAGA TAGCCTCACT GGCTCTGCAT 
CTCTTAGAGA CTGTGGGAAA TCTGAAGATT AGGCACAAGC CCACCGAAAC AGTTCAATTA 
CGTATCGGAG TTCACTCTGG TCCTTGTGCA GCAGGAGTGG TGGGTCAGAA GATGCCCAGA 
TACTGCCTAT TCGGAGACAC GGTGAACACA GCAAGTCGGA TGGAGAGCAC CGGTGACTCG 
ATGAGAATTC ATATATCCGA AGCCACCTAC CAGCTGCTCC AGGTGATCGG TAGCTATGTG 
TGCATTGAAA GGGGTCTGAC CAGCATAAAG GGCAAGGGCG ACATGCGAAC CTATTGGCTG 
ACCAAACGTC AGCAACCCGA GTTAACGCCG GATCTGATTA GCACCGTGGA TACTTTGGAC 
ACCTACTGCT CCGGGCCACG TGAGAGCATG GAGGTCTCAG TCCACCAATA TTGTAGTCCG 
GCCTCGAATA ACTACAGATT GGGATCCTGC AACTGTGACA CCAAGTGTTT ATATAGCCGT 
CGTTCGGACG ACAATGTAAC TAATTCCCAT GGAACATCCG AGTTTCCAAA GGTCAGTGAA 
CCCGCGCAGG TCAACTGCAA TCAATTATGT GTTTGCCGAT TGAACAGCAG CCAAATGTTT 
AATAATCGAG GTCCTCGATC AGCGCCTAGT ATCACATTTA GACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gyc32E ( NM_176013.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_788027.1 Drosophila melanogaster guanylyl cyclase at 32E, transcript variant D (Gyc32E), mRNA.

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CloneID	OFa21628
Clone ID Related Accession (Same CDS sequence)	NM_176013.2
Accession Version	NM_176013.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	guanylyl cyclase at 32E, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_176013.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCAGGTC CTTGCGCCTC CGCCGCTGCA TTCTCCTGCA TTCTCGTGCT CCTTCTGCTC 
GGCTGTCAGC GGAGCAATCC TCTGGCCGCC GGAGCCACCG TCTCCTCCAT GCGTCGACTG 
ACGGATACGA TAAATATTGG ATTCCTGGCT GAATATTCTC AGATGCGAGT GACACTCGGC 
GGACTGCCAC TTGCCATCGA GGATGTGAAC AAGAATCCCA ACCTATTGCC GGGCAAGAAG 
TTGGCTTTCA AACCGGTTGA CATTGGTCAC AAGATGAGCG CCTATCGCGT TAAACCGTTG 
AGGGCGATGA CACAGATGCG GGAAGCTGGC GTGACCGCAT TCATTGGTCC CGACGAGAGC 
TGCACCACGG AGGCGCTGCT GGCCTCCGCA TGGAACACAC CAATGCTATC CTTCAAATGC 
TCCGATCCAA TTGTCTCAAA CAAAAGTACA TTTCACACCT TTGCTCGAAC TTTAGCGCCG 
GCTTCCAAGG TCTCGAAAAG TGTTATTAGT CTGCTGAACG CCTTCCATTG GAATAAATTC 
TCCATAGTAG TCAGCTCGAA ACCGATTTGG GGCTCGGATG TAGCCCGTGC CATTCAGGAA 
TTGGCGGAAG CCAGGAACTT TACCATCAGC CACTTTAAAT ATATTTCGGA CTACATACCA 
ACCACAAAGA CCCTTTCGCA GATCGATAAA ATTATTGAGG AAACCTATGC CACAACTCGC 
ATCTATGTCT TCATTGGTGA GCACATTGCC ATGGTGGACT TTGTGCGAGG ACTCCAGAAT 
CGTCGGCTGC TCGAGAGTGG GGACTATATC GTGGTTTCTG TGGACGACGA GATCTACGAC 
TCCAATCGAA GGGTGAACAT TATGGAACGT AATTATTTAG ATCCTTATAT TAGAAAAGAA 
AAGAGCAAAT CGCTGGACAA AATCTCATTT CGTTCAGTTA TAAAAATAAG CATGACATAT 
CCACAAAACC CCCATATTCG TGTTCCCATA TATGGCCTAC ATCTTTACGA CAGTGTTATG 
ATCTATGTTC GAGCTATAAC TGAAGTTTTA AGACTGGGCG GTGATATTTA CGATGGAAAC 
TTGGTAATGA GTCACATCTT TAATAGGTCC TACCATTCCA TACAAGGATT TGATGTATAC 
ATAGACTCTA ATGGTGATGC TGAGGGTAAT TACACAGTCA TCACTCTGCA AAACGATGTG 
GGCAGTGGAG CATCGATAGG TTCTTTAGCC AAGATGTCAA TGCAACCAGT TGGGTTCTTT 
GCCTACGACA AGAACTCTGT TATTCCAGAA TTTCGCTACA TAAAGAATGA TAGACCCATC 
CAGTGGCTAA ATGGGCGTCC TCCGCTCGCA GAACCCCTAT GTGGTTTCCA TGGCGAACTA 
TGTCCCCGAA AGAAACTCGA CTGGCGGTAC CTGGTATCAG GTCCACTGTG TGCCCTCGTT 
GTTGTCGTGG CTATTGCACT GCTTATCAAG CATTACAGGT ATGAACAGAC TCTGGCCGGA 
CTTTTGTGGA AAGTGGACAT GAAGGATGTC ACCGTTATAA ATCTTGGCGA ATACAACAAT 
CCCACCAATA AGAATATTTT CCAAATCTGC CGTCAAAGCA TTTTAGTGGT TGGTGAGCCC 
AACAAGCGGT CCTTTACAAA TATAGCTCTA TTTCGTGGCA ATATTGTGGC CATGAAAAAG 
ATTCACAAGA AGAGCGTAGA CATTACCCGT TCAATTCGCA AGGAACTAAA ATTGATGCGA 
GAGGTGCGAC ATGAGAATAT TATCAACTTC ATTGGAGCGT CCACGGATCA CGGATCCGTG 
ATCATCTTCA CCACTTACTG TGCACGTGGC AGTCTGGAGG ACGTGCTGGC CAACGAAGAT 
TTGCACCTGG ACCACATGTT CATCTCCTCC CTCGTGTCCG ATATACTTAA GGGAATGATC 
TACCTGCACG ACTCGGAGAT TATATCCCAT GGCAATTTAC GGTCGAGCAA CTGCCTTATC 
GACTCCCGTT GGGTGTGCCA AATCTCGGAC TTTGGATTGC ACGAGCTGAA AGCTGGCCAA 
GAAGAGCCCA ACAAGAGTGA ACTGGAACTG AAACGTGCCC TCTGCATGGC GCCGGAGCTT 
TTGAGGGACG CCTACCGACC AGGACGCGGA TCCCAAAAGG GGGATGTCTA TTCCTTTGGC 
ATTTTGCTCT ACGAAATGAT TGGCCGCAAA GGACCTTGGG GTGACACGGC CTACTCAAAG 
GAGGAAATAA TTCAGTTTGT CAAGTGTCCT GAAATGCTGC AGCACGGAGT ATTCCGACCT 
GCATTGACGC ACACTCACTT GGACATTCCG GACTACATCC GTAAATGCCT GTGCCAGTGC 
TGGGATGAAG ATCCCGAGGT CAGGCCCGAT ATCCGGCTGG TTCGCATGCA TTTGAAGGAG 
CTTCAGGCTG GCTTAAAACC CAACATATTC GACAATATGC TATCGATAAT GGAGAAATAT 
GCTTACAATC TTGAGGGTTT GGTTCAGGAG CGAACCAATT TGTTGTACGA AGAGAAGAAG 
AAAACAGATA TGCTCCTTTA TCAGATGCTA CCAAGACCCG TAGCTGAACT TCTGAAGCGA 
GGAGATCCTG TCGAGGCTGA GTGCTTCGAC TGTGTCACTA TATTGTTCAG TGATATAGTG 
GGATTCACCG AACTCTGCAC CACCAGCACT CCCTTTGAGG TGGTGGAGAT GCTTAACGAT 
TGGTACACCT GCTGCGACTC CATAATATCC AATTATGATG TCTATAAGGT TGAGACAATT 
GGCGATGCCT ATATGGTTGT GTCTGGATTG CCACTGCAGA ACGGAAGTCG TCATGCTGGA 
GAGATAGCCT CACTGGCTCT GCATCTCTTA GAGACTGTGG GAAATCTGAA GATTAGGCAC 
AAGCCCACCG AAACAGTTCA ATTACGTATC GGAGTTCACT CTGGTCCTTG TGCAGCAGGA 
GTGGTGGGTC AGAAGATGCC CAGATACTGC CTATTCGGAG ACACGGTGAA CACAGCAAGT 
CGGATGGAGA GCACCGGTGA CTCGATGAGA ATTCATATAT CCGAAGCCAC CTACCAGCTG 
CTCCAGGTGA TCGGTAGCTA TGTGTGCATT GAAAGGGGTC TGACCAGCAT AAAGGGCAAG 
GGCGACATGC GAACCTATTG GCTGACCAAA CGTCAGCAAC CCGAGTTAAC GCCGGATCTG 
ATTAGCACCG TGGATACTTT GGACACCTAC TGCTCCGGGC CACGTGAGAG CATGGAGGTC 
TCAGTCCACC AATATTGTAG TCCGGCCTCG AATAACTACA GATTGGGATC CTGCAACTGT 
GACACCAAGT GTTTATATAG CCGTCGTTCG GACGACAATG TAACTAATTC CCATGGAACA 
TCCGAGTTTC CAAAGGTCAG TGAACCCGCG CAGGTCAACT GCAATCAATT ATGTGTTTGC 
CGATTGAACA GCAGCCAAAT GTTTAATAAT CGAGGTCCTC GATCAGCGCC TAGTATCACA 
TTTAGACTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788027.1
CDS	227..3718
Translation	MPGPCASAAAFSCILVLLLLGCQRSNPLAAGATVSSMRRLTDTINIGFLAEYSQMRVTLGGLPLAIEDVNKNPNLLPGKKLAFKPVDIGHKMSAYRVKPLRAMTQMREAGVTAFIGPDESCTTEALLASAWNTPMLSFKCSDPIVSNKSTFHTFARTLAPASKVSKSVISLLNAFHWNKFSIVVSSKPIWGSDVARAIQELAEARNFTISHFKYISDYIPTTKTLSQIDKIIEETYATTRIYVFIGEHIAMVDFVRGLQNRRLLESGDYIVVSVDDEIYDSNRRVNIMERNYLDPYIRKEKSKSLDKISFRSVIKISMTYPQNPHIRVPIYGLHLYDSVMIYVRAITEVLRLGGDIYDGNLVMSHIFNRSYHSIQGFDVYIDSNGDAEGNYTVITLQNDVGSGASIGSLAKMSMQPVGFFAYDKNSVIPEFRYIKNDRPIQWLNGRPPLAEPLCGFHGELCPRKKLDWRYLVSGPLCALVVVVAIALLIKHYRYEQTLAGLLWKVDMKDVTVINLGEYNNPTNKNIFQICRQSILVVGEPNKRSFTNIALFRGNIVAMKKIHKKSVDITRSIRKELKLMREVRHENIINFIGASTDHGSVIIFTTYCARGSLEDVLANEDLHLDHMFISSLVSDILKGMIYLHDSEIISHGNLRSSNCLIDSRWVCQISDFGLHELKAGQEEPNKSELELKRALCMAPELLRDAYRPGRGSQKGDVYSFGILLYEMIGRKGPWGDTAYSKEEIIQFVKCPEMLQHGVFRPALTHTHLDIPDYIRKCLCQCWDEDPEVRPDIRLVRMHLKELQAGLKPNIFDNMLSIMEKYAYNLEGLVQERTNLLYEEKKKTDMLLYQMLPRPVAELLKRGDPVEAECFDCVTILFSDIVGFTELCTTSTPFEVVEMLNDWYTCCDSIISNYDVYKVETIGDAYMVVSGLPLQNGSRHAGEIASLALHLLETVGNLKIRHKPTETVQLRIGVHSGPCAAGVVGQKMPRYCLFGDTVNTASRMESTGDSMRIHISEATYQLLQVIGSYVCIERGLTSIKGKGDMRTYWLTKRQQPELTPDLISTVDTLDTYCSGPRESMEVSVHQYCSPASNNYRLGSCNCDTKCLYSRRSDDNVTNSHGTSEFPKVSEPAQVNCNQLCVCRLNSSQMFNNRGPRSAPSITFRL

Target ORF information:

RefSeq Version	NM_176013.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster guanylyl cyclase at 32E, transcript variant D (Gyc32E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176013.2

ORF Insert Sequence:

ATGCCAGGTC CTTGCGCCTC CGCCGCTGCA TTCTCCTGCA TTCTCGTGCT CCTTCTGCTC 
GGCTGTCAGC GGAGCAATCC TCTGGCCGCC GGAGCCACCG TCTCCTCCAT GCGTCGACTG 
ACGGATACGA TAAATATTGG ATTCCTGGCT GAATATTCTC AGATGCGAGT GACACTCGGC 
GGACTGCCAC TTGCCATCGA GGATGTGAAC AAGAATCCCA ACCTATTGCC GGGCAAGAAG 
TTGGCTTTCA AACCGGTTGA CATTGGTCAC AAGATGAGCG CCTATCGCGT TAAACCGTTG 
AGGGCGATGA CACAGATGCG GGAAGCTGGC GTGACCGCAT TCATTGGTCC CGACGAGAGC 
TGCACCACGG AGGCGCTGCT GGCCTCCGCA TGGAACACAC CAATGCTATC CTTCAAATGC 
TCCGATCCAA TTGTCTCAAA CAAAAGTACA TTTCACACCT TTGCTCGAAC TTTAGCGCCG 
GCTTCCAAGG TCTCGAAAAG TGTTATTAGT CTGCTGAACG CCTTCCATTG GAATAAATTC 
TCCATAGTAG TCAGCTCGAA ACCGATTTGG GGCTCGGATG TAGCCCGTGC CATTCAGGAA 
TTGGCGGAAG CCAGGAACTT TACCATCAGC CACTTTAAAT ATATTTCGGA CTACATACCA 
ACCACAAAGA CCCTTTCGCA GATCGATAAA ATTATTGAGG AAACCTATGC CACAACTCGC 
ATCTATGTCT TCATTGGTGA GCACATTGCC ATGGTGGACT TTGTGCGAGG ACTCCAGAAT 
CGTCGGCTGC TCGAGAGTGG GGACTATATC GTGGTTTCTG TGGACGACGA GATCTACGAC 
TCCAATCGAA GGGTGAACAT TATGGAACGT AATTATTTAG ATCCTTATAT TAGAAAAGAA 
AAGAGCAAAT CGCTGGACAA AATCTCATTT CGTTCAGTTA TAAAAATAAG CATGACATAT 
CCACAAAACC CCCATATTCG TGTTCCCATA TATGGCCTAC ATCTTTACGA CAGTGTTATG 
ATCTATGTTC GAGCTATAAC TGAAGTTTTA AGACTGGGCG GTGATATTTA CGATGGAAAC 
TTGGTAATGA GTCACATCTT TAATAGGTCC TACCATTCCA TACAAGGATT TGATGTATAC 
ATAGACTCTA ATGGTGATGC TGAGGGTAAT TACACAGTCA TCACTCTGCA AAACGATGTG 
GGCAGTGGAG CATCGATAGG TTCTTTAGCC AAGATGTCAA TGCAACCAGT TGGGTTCTTT 
GCCTACGACA AGAACTCTGT TATTCCAGAA TTTCGCTACA TAAAGAATGA TAGACCCATC 
CAGTGGCTAA ATGGGCGTCC TCCGCTCGCA GAACCCCTAT GTGGTTTCCA TGGCGAACTA 
TGTCCCCGAA AGAAACTCGA CTGGCGGTAC CTGGTATCAG GTCCACTGTG TGCCCTCGTT 
GTTGTCGTGG CTATTGCACT GCTTATCAAG CATTACAGGT ATGAACAGAC TCTGGCCGGA 
CTTTTGTGGA AAGTGGACAT GAAGGATGTC ACCGTTATAA ATCTTGGCGA ATACAACAAT 
CCCACCAATA AGAATATTTT CCAAATCTGC CGTCAAAGCA TTTTAGTGGT TGGTGAGCCC 
AACAAGCGGT CCTTTACAAA TATAGCTCTA TTTCGTGGCA ATATTGTGGC CATGAAAAAG 
ATTCACAAGA AGAGCGTAGA CATTACCCGT TCAATTCGCA AGGAACTAAA ATTGATGCGA 
GAGGTGCGAC ATGAGAATAT TATCAACTTC ATTGGAGCGT CCACGGATCA CGGATCCGTG 
ATCATCTTCA CCACTTACTG TGCACGTGGC AGTCTGGAGG ACGTGCTGGC CAACGAAGAT 
TTGCACCTGG ACCACATGTT CATCTCCTCC CTCGTGTCCG ATATACTTAA GGGAATGATC 
TACCTGCACG ACTCGGAGAT TATATCCCAT GGCAATTTAC GGTCGAGCAA CTGCCTTATC 
GACTCCCGTT GGGTGTGCCA AATCTCGGAC TTTGGATTGC ACGAGCTGAA AGCTGGCCAA 
GAAGAGCCCA ACAAGAGTGA ACTGGAACTG AAACGTGCCC TCTGCATGGC GCCGGAGCTT 
TTGAGGGACG CCTACCGACC AGGACGCGGA TCCCAAAAGG GGGATGTCTA TTCCTTTGGC 
ATTTTGCTCT ACGAAATGAT TGGCCGCAAA GGACCTTGGG GTGACACGGC CTACTCAAAG 
GAGGAAATAA TTCAGTTTGT CAAGTGTCCT GAAATGCTGC AGCACGGAGT ATTCCGACCT 
GCATTGACGC ACACTCACTT GGACATTCCG GACTACATCC GTAAATGCCT GTGCCAGTGC 
TGGGATGAAG ATCCCGAGGT CAGGCCCGAT ATCCGGCTGG TTCGCATGCA TTTGAAGGAG 
CTTCAGGCTG GCTTAAAACC CAACATATTC GACAATATGC TATCGATAAT GGAGAAATAT 
GCTTACAATC TTGAGGGTTT GGTTCAGGAG CGAACCAATT TGTTGTACGA AGAGAAGAAG 
AAAACAGATA TGCTCCTTTA TCAGATGCTA CCAAGACCCG TAGCTGAACT TCTGAAGCGA 
GGAGATCCTG TCGAGGCTGA GTGCTTCGAC TGTGTCACTA TATTGTTCAG TGATATAGTG 
GGATTCACCG AACTCTGCAC CACCAGCACT CCCTTTGAGG TGGTGGAGAT GCTTAACGAT 
TGGTACACCT GCTGCGACTC CATAATATCC AATTATGATG TCTATAAGGT TGAGACAATT 
GGCGATGCCT ATATGGTTGT GTCTGGATTG CCACTGCAGA ACGGAAGTCG TCATGCTGGA 
GAGATAGCCT CACTGGCTCT GCATCTCTTA GAGACTGTGG GAAATCTGAA GATTAGGCAC 
AAGCCCACCG AAACAGTTCA ATTACGTATC GGAGTTCACT CTGGTCCTTG TGCAGCAGGA 
GTGGTGGGTC AGAAGATGCC CAGATACTGC CTATTCGGAG ACACGGTGAA CACAGCAAGT 
CGGATGGAGA GCACCGGTGA CTCGATGAGA ATTCATATAT CCGAAGCCAC CTACCAGCTG 
CTCCAGGTGA TCGGTAGCTA TGTGTGCATT GAAAGGGGTC TGACCAGCAT AAAGGGCAAG 
GGCGACATGC GAACCTATTG GCTGACCAAA CGTCAGCAAC CCGAGTTAAC GCCGGATCTG 
ATTAGCACCG TGGATACTTT GGACACCTAC TGCTCCGGGC CACGTGAGAG CATGGAGGTC 
TCAGTCCACC AATATTGTAG TCCGGCCTCG AATAACTACA GATTGGGATC CTGCAACTGT 
GACACCAAGT GTTTATATAG CCGTCGTTCG GACGACAATG TAACTAATTC CCATGGAACA 
TCCGAGTTTC CAAAGGTCAG TGAACCCGCG CAGGTCAACT GCAATCAATT ATGTGTTTGC 
CGATTGAACA GCAGCCAAAT GTTTAATAAT CGAGGTCCTC GATCAGCGCC TAGTATCACA 
TTTAGACTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Gyc32E cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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