RluA-1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	RluA-1
Entrez Gene ID	34438
Full Name	CG31719 gene product from transcript CG31719-RD
Synonyms	CG13143,CG31719,CG7475,Dmel\CG31719,RluA1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 31E6-31F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001273422.1	NP_001260351.1	RluA pseudouridine synthase 1, isoform D
NM_164931.2	NP_723593.1	RluA pseudouridine synthase 1, isoform B
NM_164930.2	NP_723592.1	RluA pseudouridine synthase 1, isoform A
NM_164932.2	NP_723594.1	RluA pseudouridine synthase 1, isoform C

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	LOC100911166	XP_003749602.1
Xenopus tropicalis (tropical clawed frog)	rpusd2	XP_002940545.1
Rattus norvegicus (Norway rat)	Rpusd2	NP_001129317.1
Drosophila melanogaster (fruit fly)	RluA-1	NP_723594.1
Caenorhabditis elegans (roundworm)	CELE_K07E8.7	NP_493778.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009693	XP_553504.3
Arabidopsis thaliana (thale cress)	AT5G51140	NP_199927.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the membrane-tethered green fluorescent protein driven by RluA-1-GAL4 was expressed in the dendritic arbors of MD neurons, confirming that RluA-1 is indeed expressed in MD neurons.
Title: Spatially controlled expression of the Drosophila pseudouridine synthase RluA-1.

The following RluA-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RluA-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa15984	NM_001273422.1 Latest version!	Drosophila melanogaster RluA pseudouridine synthase 1 (RluA-1), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa14364	NM_164930.2 Latest version!	Drosophila melanogaster RluA pseudouridine synthase 1 (RluA-1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa14364	NM_164932.2 Latest version!	Drosophila melanogaster RluA pseudouridine synthase 1 (RluA-1), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa14364	NM_164931.2 Latest version!	Drosophila melanogaster RluA pseudouridine synthase 1 (RluA-1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RluA-1 ( NM_001273422.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260351.1 Drosophila melanogaster RluA pseudouridine synthase 1 (RluA-1), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa15984
Clone ID Related Accession (Same CDS sequence)	NM_001273422.1
Accession Version	NM_001273422.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	RluA pseudouridine synthase 1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGAATT CTCCGGCTCC GTGTGCCTGG TACTTGCCCT GGTCCCTGGC CGCCCAGCAG 
CACCAGCAAA AGATGCTGCA AATGCAGTCG CCGTTTCTGG ACAAGATGGG CGCCACATCG 
GTGGGCGGCA TCTTCGCTGG CCAGCCGCAG ATGCAGCAAC AATTGTCGCC CAATACGGCA 
GCAGCACCGC CGGCAAACTA TCAGCAGCCC GCTTTGCATC CAAGCGCCGC ACCAGGCGCA 
CCACACTTCC ACATGGGATC CCCGTATAGC CATCTGGCAC CGCAGCTCCT CAACGCCGGA 
CAGCTGAACC AGAACGCACT GATGCACTCC GCCATGTTCT CTTCCCTGCC ACTTGGTGCG 
TACTATGCAC CCGCCGCCGG CGCAGGTCAC TCGGCCTTTG GTGGCGTTCC CCTGACCACG 
GCTGCCCAGC AATCTCTATT GGCCGCCACC GGAGGAGCAA CTGCTGGCCA TTTGGCCAAC 
CAGCAGACGA CGGCTCAAGT GCCCGTCCAG GTGCCCGTGC AAATGGCCCA ACGGACAGCT 
CCGGCCGCCT GCTCCATGGT CCAGCCACTT AACTGCCTGC CGCACCAGGA ACTGAATCAC 
CTGTCGTCCA TCAATCTCAA CCTGCTGCGC AGTCCGGCGC CTCCGCTCCC AGCCATTCAG 
GTCTTGCCAA GTGCCGAGGT GCCGATTAAT AAGAAGACTA CTTCTCCCAG GGACTCTGAG 
AATCTTAAAA TCAAGGTTCC AGAAAAGCGG AAAACAGACG ATAATGATCA GATAAAAGAG 
CTAAAGAAAG CTAAACTGGA GACAAAAGCA TTGAAGGCTA AGAGGCCGGG ATTCACGGAT 
GAAAGATACC AGGAAACATC CTACTATATG GAAAACGGTC TACGCAAGGT GTATCCGTAC 
TACTTTACAT TCACAACATT TACAAAAGGT CGCTGGGTGG GCGAAAAGAT ATTGGATATA 
TTCTCGCGAG AATTTCGGGC ACATCCCGCT GAGGAATATG AACGTTGTAT CCAAACTGGC 
ACATTGACAG TCAACTTTGA GAAGGTACCC ATTGACTATC GGCTGAAACA TAACGATCTT 
CTCGCCAATA TAGTTCACAG GTGCATCCGT GTGGACGTTA TAGGCACAAC ACCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260351.1
CDS	113..1249
Translation	MQNSPAPCAWYLPWSLAAQQHQQKMLQMQSPFLDKMGATSVGGIFAGQPQMQQQLSPNTAAAPPANYQQPALHPSAAPGAPHFHMGSPYSHLAPQLLNAGQLNQNALMHSAMFSSLPLGAYYAPAAGAGHSAFGGVPLTTAAQQSLLAATGGATAGHLANQQTTAQVPVQVPVQMAQRTAPAACSMVQPLNCLPHQELNHLSSINLNLLRSPAPPLPAIQVLPSAEVPINKKTTSPRDSENLKIKVPEKRKTDDNDQIKELKKAKLETKALKAKRPGFTDERYQETSYYMENGLRKVYPYYFTFTTFTKGRWVGEKILDIFSREFRAHPAEEYERCIQTGTLTVNFEKVPIDYRLKHNDLLANIVHRCIRVDVIGTTP

Target ORF information:

RefSeq Version	NM_001273422.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster RluA pseudouridine synthase 1 (RluA-1), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273422.1

ORF Insert Sequence:

ATGCAGAATT CTCCGGCTCC GTGTGCCTGG TACTTGCCCT GGTCCCTGGC CGCCCAGCAG 
CACCAGCAAA AGATGCTGCA AATGCAGTCG CCGTTTCTGG ACAAGATGGG CGCCACATCG 
GTGGGCGGCA TCTTCGCTGG CCAGCCGCAG ATGCAGCAAC AATTGTCGCC CAATACGGCA 
GCAGCACCGC CGGCAAACTA TCAGCAGCCC GCTTTGCATC CAAGCGCCGC ACCAGGCGCA 
CCACACTTCC ACATGGGATC CCCGTATAGC CATCTGGCAC CGCAGCTCCT CAACGCCGGA 
CAGCTGAACC AGAACGCACT GATGCACTCC GCCATGTTCT CTTCCCTGCC ACTTGGTGCG 
TACTATGCAC CCGCCGCCGG CGCAGGTCAC TCGGCCTTTG GTGGCGTTCC CCTGACCACG 
GCTGCCCAGC AATCTCTATT GGCCGCCACC GGAGGAGCAA CTGCTGGCCA TTTGGCCAAC 
CAGCAGACGA CGGCTCAAGT GCCCGTCCAG GTGCCCGTGC AAATGGCCCA ACGGACAGCT 
CCGGCCGCCT GCTCCATGGT CCAGCCACTT AACTGCCTGC CGCACCAGGA ACTGAATCAC 
CTGTCGTCCA TCAATCTCAA CCTGCTGCGC AGTCCGGCGC CTCCGCTCCC AGCCATTCAG 
GTCTTGCCAA GTGCCGAGGT GCCGATTAAT AAGAAGACTA CTTCTCCCAG GGACTCTGAG 
AATCTTAAAA TCAAGGTTCC AGAAAAGCGG AAAACAGACG ATAATGATCA GATAAAAGAG 
CTAAAGAAAG CTAAACTGGA GACAAAAGCA TTGAAGGCTA AGAGGCCGGG ATTCACGGAT 
GAAAGATACC AGGAAACATC CTACTATATG GAAAACGGTC TACGCAAGGT GTATCCGTAC 
TACTTTACAT TCACAACATT TACAAAAGGT CGCTGGGTGG GCGAAAAGAT ATTGGATATA 
TTCTCGCGAG AATTTCGGGC ACATCCCGCT GAGGAATATG AACGTTGTAT CCAAACTGGC 
ACATTGACAG TCAACTTTGA GAAGGTACCC ATTGACTATC GGCTGAAACA TAACGATCTT 
CTCGCCAATA TAGTTCACAG GTGCATCCGT GTGGACGTTA TAGGCACAAC ACCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RluA-1 ( NM_164931.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723593.1 Drosophila melanogaster RluA pseudouridine synthase 1 (RluA-1), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa14364
Clone ID Related Accession (Same CDS sequence)	NM_164931.2 , NM_164930.2 , NM_164932.2
Accession Version	NM_164931.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2220bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	RluA pseudouridine synthase 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164931.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGAATT CTCCGGCTCC GTGTGCCTGG TACTTGCCCT GGTCCCTGGC CGCCCAGCAG 
CACCAGCAAA AGATGCTGCA AATGCAGTCG CCGTTTCTGG ACAAGATGGG CGCCACATCG 
GTGGGCGGCA TCTTCGCTGG CCAGCCGCAG ATGCAGCAAC AATTGTCGCC CAATACGGCA 
GCAGCACCGC CGGCAAACTA TCAGCAGCCC GCTTTGCATC CAAGCGCCGC ACCAGGCGCA 
CCACACTTCC ACATGGGATC CCCGTATAGC CATCTGGCAC CGCAGCTCCT CAACGCCGGA 
CAGCTGAACC AGAACGCACT GATGCACTCC GCCATGTTCT CTTCCCTGCC ACTTGGTGCG 
TACTATGCAC CCGCCGCCGG CGCAGGTCAC TCGGCCTTTG GTGGCGTTCC CCTGACCACG 
GCTGCCCAGC AATCTCTATT GGCCGCCACC GGAGGAGCAA CTGCTGGCCA TTTGGCCAAC 
CAGCAGACGA CGGCTCAAGT GCCCGTCCAG GTGCCCGTGC AAATGGCCCA ACGGACAGCT 
CCGGCCGCCT GCTCCATGGT CCAGCCACTT AACTGCCTGC CGCACCAGGA ACTGAATCAC 
CTGTCGTCCA TCAATCTCAA CCTGCTGCGC AGTCCGGCGC CTCCGCTCCC AGCCATTCAG 
GTCTTGCCAA GTGCCGAGGT GCCGATTAAT AAGAAGACTA CTTCTCCCAG GGACTCTGAG 
AATCTTAAAA TCAAGGTTCC AGAAAAGCGG AAAACAGACG ATAATGATCA GATAAAAGAG 
CTAAAGAAAG CTAAACTGGA GACAAAAGCA TTGAAGGCTA AGAGGCCGGG ATTCACGGAT 
GAAAGATACC AGGAAACATC CTACTATATG GAAAACGGTC TACGCAAGGT GTATCCGTAC 
TACTTTACAT TCACAACATT TACAAAAGGT CGCTGGGTGG GCGAAAAGAT ATTGGATATA 
TTCTCGCGAG AATTTCGGGC ACATCCCGCT GAGGAATATG AACGTTGTAT CCAAACTGGC 
ACATTGACAG TCAACTTTGA GAAGGTACCC ATTGACTATC GGCTGAAACA TAACGATCTT 
CTCGCCAATA TAGTTCACAG ACACGAAGTT CCTGTGACTT GTCAACCAAT TAAAATTGTG 
CATATGGATG AGGACATTGT AGTTGTAAAT AAGCCAGCAT CGATACCTGT GCATCCGTGT 
GGACGTTATA GGCACAACAC CGTAGTCTTC ATTCTGGCCA AGGAGTTCAA TCTCAAGAAT 
CTACGCACAA TTCACCGACT AGATCGGTTG ACCTCGGGAC TCCTACTTTT TGGACGCAGT 
CCGAAGAAGG CGCGCCAAAT GGAGCAGCAG ATTCGCAACA GGCAGGTGGA GAAGGAGTAC 
ATCTGCAGGG TGGAGGGCGT TTTTCCAGAT GGCATCGTGG AGTGCAAGGA GCCCATTGAA 
GTAGTTAGCT ACAAGATTGG CGTGTGCAAA GTATCGGCCA AGGGCAAGGA TTGCACGACT 
ACCTTCCAGA AGCTATCCCA AAATGGCACC ACCAGTGTGG TGCTCTGCAA ACCACTCACT 
GGCCGAATGC ATCAGATAAG AGTGCACTTG CAATATTTAG GTTATCCCAT TCTAAACGAT 
CCACTGTACA ATCACGAAGT CTTCGGTCCG CTGAAAGGAC GATCCGGAGA TATCGGTGGC 
AAGTCGGATG AAGAGCTTAT ACGAGATCTC ATAAACATAC ACAATGCAGA GAACTGGCTG 
GGCATAGATT GTGATTCGGA TATATCCATG TTCAAGAGCA CCAAGGATGA GGCAGATCGG 
GAAAGTCTCA GCAGTGAGCA CACTTCCGTT GTTCATCATA GCGATGATGA TGGATGCGTC 
AATTCGCGGG AAACTACACC ACCTTGCAAT GAGCCACAGC AGCCGGAAAA TAGTGTTAAA 
CTTTTAGAAA CTACAAATGC AGTCAAGGAG TATCAAGTTG CGGCACAGAA GTCTTCCTCG 
GAAATTTGCC CAGCTCCACT GGATGCAGTG GAGAGTCCAC TGAATGGAGG TGGATGCAAT 
GTGGGCAAGG TGACAGTAGA TGAGCATTGC TATGAGTGCA AAGTGCACTA CCGTGATCCA 
AAGTCAAAGG ACCTTATAAT GTATCTGCAC GCATGGAAGT ACAAGGGCCC TGGATGGGAG 
TACGAAACTG AGTTACCGAA TTGGGCCCGC AACGATTGGG ACCACTTAGA CTCGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723593.1
CDS	517..2736
Translation	MQNSPAPCAWYLPWSLAAQQHQQKMLQMQSPFLDKMGATSVGGIFAGQPQMQQQLSPNTAAAPPANYQQPALHPSAAPGAPHFHMGSPYSHLAPQLLNAGQLNQNALMHSAMFSSLPLGAYYAPAAGAGHSAFGGVPLTTAAQQSLLAATGGATAGHLANQQTTAQVPVQVPVQMAQRTAPAACSMVQPLNCLPHQELNHLSSINLNLLRSPAPPLPAIQVLPSAEVPINKKTTSPRDSENLKIKVPEKRKTDDNDQIKELKKAKLETKALKAKRPGFTDERYQETSYYMENGLRKVYPYYFTFTTFTKGRWVGEKILDIFSREFRAHPAEEYERCIQTGTLTVNFEKVPIDYRLKHNDLLANIVHRHEVPVTCQPIKIVHMDEDIVVVNKPASIPVHPCGRYRHNTVVFILAKEFNLKNLRTIHRLDRLTSGLLLFGRSPKKARQMEQQIRNRQVEKEYICRVEGVFPDGIVECKEPIEVVSYKIGVCKVSAKGKDCTTTFQKLSQNGTTSVVLCKPLTGRMHQIRVHLQYLGYPILNDPLYNHEVFGPLKGRSGDIGGKSDEELIRDLINIHNAENWLGIDCDSDISMFKSTKDEADRESLSSEHTSVVHHSDDDGCVNSRETTPPCNEPQQPENSVKLLETTNAVKEYQVAAQKSSSEICPAPLDAVESPLNGGGCNVGKVTVDEHCYECKVHYRDPKSKDLIMYLHAWKYKGPGWEYETELPNWARNDWDHLDSA

Target ORF information:

RefSeq Version	NM_164931.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster RluA pseudouridine synthase 1 (RluA-1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164931.2

ORF Insert Sequence:

ATGCAGAATT CTCCGGCTCC GTGTGCCTGG TACTTGCCCT GGTCCCTGGC CGCCCAGCAG 
CACCAGCAAA AGATGCTGCA AATGCAGTCG CCGTTTCTGG ACAAGATGGG CGCCACATCG 
GTGGGCGGCA TCTTCGCTGG CCAGCCGCAG ATGCAGCAAC AATTGTCGCC CAATACGGCA 
GCAGCACCGC CGGCAAACTA TCAGCAGCCC GCTTTGCATC CAAGCGCCGC ACCAGGCGCA 
CCACACTTCC ACATGGGATC CCCGTATAGC CATCTGGCAC CGCAGCTCCT CAACGCCGGA 
CAGCTGAACC AGAACGCACT GATGCACTCC GCCATGTTCT CTTCCCTGCC ACTTGGTGCG 
TACTATGCAC CCGCCGCCGG CGCAGGTCAC TCGGCCTTTG GTGGCGTTCC CCTGACCACG 
GCTGCCCAGC AATCTCTATT GGCCGCCACC GGAGGAGCAA CTGCTGGCCA TTTGGCCAAC 
CAGCAGACGA CGGCTCAAGT GCCCGTCCAG GTGCCCGTGC AAATGGCCCA ACGGACAGCT 
CCGGCCGCCT GCTCCATGGT CCAGCCACTT AACTGCCTGC CGCACCAGGA ACTGAATCAC 
CTGTCGTCCA TCAATCTCAA CCTGCTGCGC AGTCCGGCGC CTCCGCTCCC AGCCATTCAG 
GTCTTGCCAA GTGCCGAGGT GCCGATTAAT AAGAAGACTA CTTCTCCCAG GGACTCTGAG 
AATCTTAAAA TCAAGGTTCC AGAAAAGCGG AAAACAGACG ATAATGATCA GATAAAAGAG 
CTAAAGAAAG CTAAACTGGA GACAAAAGCA TTGAAGGCTA AGAGGCCGGG ATTCACGGAT 
GAAAGATACC AGGAAACATC CTACTATATG GAAAACGGTC TACGCAAGGT GTATCCGTAC 
TACTTTACAT TCACAACATT TACAAAAGGT CGCTGGGTGG GCGAAAAGAT ATTGGATATA 
TTCTCGCGAG AATTTCGGGC ACATCCCGCT GAGGAATATG AACGTTGTAT CCAAACTGGC 
ACATTGACAG TCAACTTTGA GAAGGTACCC ATTGACTATC GGCTGAAACA TAACGATCTT 
CTCGCCAATA TAGTTCACAG ACACGAAGTT CCTGTGACTT GTCAACCAAT TAAAATTGTG 
CATATGGATG AGGACATTGT AGTTGTAAAT AAGCCAGCAT CGATACCTGT GCATCCGTGT 
GGACGTTATA GGCACAACAC CGTAGTCTTC ATTCTGGCCA AGGAGTTCAA TCTCAAGAAT 
CTACGCACAA TTCACCGACT AGATCGGTTG ACCTCGGGAC TCCTACTTTT TGGACGCAGT 
CCGAAGAAGG CGCGCCAAAT GGAGCAGCAG ATTCGCAACA GGCAGGTGGA GAAGGAGTAC 
ATCTGCAGGG TGGAGGGCGT TTTTCCAGAT GGCATCGTGG AGTGCAAGGA GCCCATTGAA 
GTAGTTAGCT ACAAGATTGG CGTGTGCAAA GTATCGGCCA AGGGCAAGGA TTGCACGACT 
ACCTTCCAGA AGCTATCCCA AAATGGCACC ACCAGTGTGG TGCTCTGCAA ACCACTCACT 
GGCCGAATGC ATCAGATAAG AGTGCACTTG CAATATTTAG GTTATCCCAT TCTAAACGAT 
CCACTGTACA ATCACGAAGT CTTCGGTCCG CTGAAAGGAC GATCCGGAGA TATCGGTGGC 
AAGTCGGATG AAGAGCTTAT ACGAGATCTC ATAAACATAC ACAATGCAGA GAACTGGCTG 
GGCATAGATT GTGATTCGGA TATATCCATG TTCAAGAGCA CCAAGGATGA GGCAGATCGG 
GAAAGTCTCA GCAGTGAGCA CACTTCCGTT GTTCATCATA GCGATGATGA TGGATGCGTC 
AATTCGCGGG AAACTACACC ACCTTGCAAT GAGCCACAGC AGCCGGAAAA TAGTGTTAAA 
CTTTTAGAAA CTACAAATGC AGTCAAGGAG TATCAAGTTG CGGCACAGAA GTCTTCCTCG 
GAAATTTGCC CAGCTCCACT GGATGCAGTG GAGAGTCCAC TGAATGGAGG TGGATGCAAT 
GTGGGCAAGG TGACAGTAGA TGAGCATTGC TATGAGTGCA AAGTGCACTA CCGTGATCCA 
AAGTCAAAGG ACCTTATAAT GTATCTGCAC GCATGGAAGT ACAAGGGCCC TGGATGGGAG 
TACGAAACTG AGTTACCGAA TTGGGCCCGC AACGATTGGG ACCACTTAGA CTCGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RluA-1 ( NM_164930.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723592.1 Drosophila melanogaster RluA pseudouridine synthase 1 (RluA-1), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa14364
Clone ID Related Accession (Same CDS sequence)	NM_164931.2 , NM_164930.2 , NM_164932.2
Accession Version	NM_164930.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2220bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	RluA pseudouridine synthase 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164930.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGAATT CTCCGGCTCC GTGTGCCTGG TACTTGCCCT GGTCCCTGGC CGCCCAGCAG 
CACCAGCAAA AGATGCTGCA AATGCAGTCG CCGTTTCTGG ACAAGATGGG CGCCACATCG 
GTGGGCGGCA TCTTCGCTGG CCAGCCGCAG ATGCAGCAAC AATTGTCGCC CAATACGGCA 
GCAGCACCGC CGGCAAACTA TCAGCAGCCC GCTTTGCATC CAAGCGCCGC ACCAGGCGCA 
CCACACTTCC ACATGGGATC CCCGTATAGC CATCTGGCAC CGCAGCTCCT CAACGCCGGA 
CAGCTGAACC AGAACGCACT GATGCACTCC GCCATGTTCT CTTCCCTGCC ACTTGGTGCG 
TACTATGCAC CCGCCGCCGG CGCAGGTCAC TCGGCCTTTG GTGGCGTTCC CCTGACCACG 
GCTGCCCAGC AATCTCTATT GGCCGCCACC GGAGGAGCAA CTGCTGGCCA TTTGGCCAAC 
CAGCAGACGA CGGCTCAAGT GCCCGTCCAG GTGCCCGTGC AAATGGCCCA ACGGACAGCT 
CCGGCCGCCT GCTCCATGGT CCAGCCACTT AACTGCCTGC CGCACCAGGA ACTGAATCAC 
CTGTCGTCCA TCAATCTCAA CCTGCTGCGC AGTCCGGCGC CTCCGCTCCC AGCCATTCAG 
GTCTTGCCAA GTGCCGAGGT GCCGATTAAT AAGAAGACTA CTTCTCCCAG GGACTCTGAG 
AATCTTAAAA TCAAGGTTCC AGAAAAGCGG AAAACAGACG ATAATGATCA GATAAAAGAG 
CTAAAGAAAG CTAAACTGGA GACAAAAGCA TTGAAGGCTA AGAGGCCGGG ATTCACGGAT 
GAAAGATACC AGGAAACATC CTACTATATG GAAAACGGTC TACGCAAGGT GTATCCGTAC 
TACTTTACAT TCACAACATT TACAAAAGGT CGCTGGGTGG GCGAAAAGAT ATTGGATATA 
TTCTCGCGAG AATTTCGGGC ACATCCCGCT GAGGAATATG AACGTTGTAT CCAAACTGGC 
ACATTGACAG TCAACTTTGA GAAGGTACCC ATTGACTATC GGCTGAAACA TAACGATCTT 
CTCGCCAATA TAGTTCACAG ACACGAAGTT CCTGTGACTT GTCAACCAAT TAAAATTGTG 
CATATGGATG AGGACATTGT AGTTGTAAAT AAGCCAGCAT CGATACCTGT GCATCCGTGT 
GGACGTTATA GGCACAACAC CGTAGTCTTC ATTCTGGCCA AGGAGTTCAA TCTCAAGAAT 
CTACGCACAA TTCACCGACT AGATCGGTTG ACCTCGGGAC TCCTACTTTT TGGACGCAGT 
CCGAAGAAGG CGCGCCAAAT GGAGCAGCAG ATTCGCAACA GGCAGGTGGA GAAGGAGTAC 
ATCTGCAGGG TGGAGGGCGT TTTTCCAGAT GGCATCGTGG AGTGCAAGGA GCCCATTGAA 
GTAGTTAGCT ACAAGATTGG CGTGTGCAAA GTATCGGCCA AGGGCAAGGA TTGCACGACT 
ACCTTCCAGA AGCTATCCCA AAATGGCACC ACCAGTGTGG TGCTCTGCAA ACCACTCACT 
GGCCGAATGC ATCAGATAAG AGTGCACTTG CAATATTTAG GTTATCCCAT TCTAAACGAT 
CCACTGTACA ATCACGAAGT CTTCGGTCCG CTGAAAGGAC GATCCGGAGA TATCGGTGGC 
AAGTCGGATG AAGAGCTTAT ACGAGATCTC ATAAACATAC ACAATGCAGA GAACTGGCTG 
GGCATAGATT GTGATTCGGA TATATCCATG TTCAAGAGCA CCAAGGATGA GGCAGATCGG 
GAAAGTCTCA GCAGTGAGCA CACTTCCGTT GTTCATCATA GCGATGATGA TGGATGCGTC 
AATTCGCGGG AAACTACACC ACCTTGCAAT GAGCCACAGC AGCCGGAAAA TAGTGTTAAA 
CTTTTAGAAA CTACAAATGC AGTCAAGGAG TATCAAGTTG CGGCACAGAA GTCTTCCTCG 
GAAATTTGCC CAGCTCCACT GGATGCAGTG GAGAGTCCAC TGAATGGAGG TGGATGCAAT 
GTGGGCAAGG TGACAGTAGA TGAGCATTGC TATGAGTGCA AAGTGCACTA CCGTGATCCA 
AAGTCAAAGG ACCTTATAAT GTATCTGCAC GCATGGAAGT ACAAGGGCCC TGGATGGGAG 
TACGAAACTG AGTTACCGAA TTGGGCCCGC AACGATTGGG ACCACTTAGA CTCGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723592.1
CDS	383..2602
Translation	MQNSPAPCAWYLPWSLAAQQHQQKMLQMQSPFLDKMGATSVGGIFAGQPQMQQQLSPNTAAAPPANYQQPALHPSAAPGAPHFHMGSPYSHLAPQLLNAGQLNQNALMHSAMFSSLPLGAYYAPAAGAGHSAFGGVPLTTAAQQSLLAATGGATAGHLANQQTTAQVPVQVPVQMAQRTAPAACSMVQPLNCLPHQELNHLSSINLNLLRSPAPPLPAIQVLPSAEVPINKKTTSPRDSENLKIKVPEKRKTDDNDQIKELKKAKLETKALKAKRPGFTDERYQETSYYMENGLRKVYPYYFTFTTFTKGRWVGEKILDIFSREFRAHPAEEYERCIQTGTLTVNFEKVPIDYRLKHNDLLANIVHRHEVPVTCQPIKIVHMDEDIVVVNKPASIPVHPCGRYRHNTVVFILAKEFNLKNLRTIHRLDRLTSGLLLFGRSPKKARQMEQQIRNRQVEKEYICRVEGVFPDGIVECKEPIEVVSYKIGVCKVSAKGKDCTTTFQKLSQNGTTSVVLCKPLTGRMHQIRVHLQYLGYPILNDPLYNHEVFGPLKGRSGDIGGKSDEELIRDLINIHNAENWLGIDCDSDISMFKSTKDEADRESLSSEHTSVVHHSDDDGCVNSRETTPPCNEPQQPENSVKLLETTNAVKEYQVAAQKSSSEICPAPLDAVESPLNGGGCNVGKVTVDEHCYECKVHYRDPKSKDLIMYLHAWKYKGPGWEYETELPNWARNDWDHLDSA

Target ORF information:

RefSeq Version	NM_164930.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster RluA pseudouridine synthase 1 (RluA-1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164930.2

ORF Insert Sequence:

ATGCAGAATT CTCCGGCTCC GTGTGCCTGG TACTTGCCCT GGTCCCTGGC CGCCCAGCAG 
CACCAGCAAA AGATGCTGCA AATGCAGTCG CCGTTTCTGG ACAAGATGGG CGCCACATCG 
GTGGGCGGCA TCTTCGCTGG CCAGCCGCAG ATGCAGCAAC AATTGTCGCC CAATACGGCA 
GCAGCACCGC CGGCAAACTA TCAGCAGCCC GCTTTGCATC CAAGCGCCGC ACCAGGCGCA 
CCACACTTCC ACATGGGATC CCCGTATAGC CATCTGGCAC CGCAGCTCCT CAACGCCGGA 
CAGCTGAACC AGAACGCACT GATGCACTCC GCCATGTTCT CTTCCCTGCC ACTTGGTGCG 
TACTATGCAC CCGCCGCCGG CGCAGGTCAC TCGGCCTTTG GTGGCGTTCC CCTGACCACG 
GCTGCCCAGC AATCTCTATT GGCCGCCACC GGAGGAGCAA CTGCTGGCCA TTTGGCCAAC 
CAGCAGACGA CGGCTCAAGT GCCCGTCCAG GTGCCCGTGC AAATGGCCCA ACGGACAGCT 
CCGGCCGCCT GCTCCATGGT CCAGCCACTT AACTGCCTGC CGCACCAGGA ACTGAATCAC 
CTGTCGTCCA TCAATCTCAA CCTGCTGCGC AGTCCGGCGC CTCCGCTCCC AGCCATTCAG 
GTCTTGCCAA GTGCCGAGGT GCCGATTAAT AAGAAGACTA CTTCTCCCAG GGACTCTGAG 
AATCTTAAAA TCAAGGTTCC AGAAAAGCGG AAAACAGACG ATAATGATCA GATAAAAGAG 
CTAAAGAAAG CTAAACTGGA GACAAAAGCA TTGAAGGCTA AGAGGCCGGG ATTCACGGAT 
GAAAGATACC AGGAAACATC CTACTATATG GAAAACGGTC TACGCAAGGT GTATCCGTAC 
TACTTTACAT TCACAACATT TACAAAAGGT CGCTGGGTGG GCGAAAAGAT ATTGGATATA 
TTCTCGCGAG AATTTCGGGC ACATCCCGCT GAGGAATATG AACGTTGTAT CCAAACTGGC 
ACATTGACAG TCAACTTTGA GAAGGTACCC ATTGACTATC GGCTGAAACA TAACGATCTT 
CTCGCCAATA TAGTTCACAG ACACGAAGTT CCTGTGACTT GTCAACCAAT TAAAATTGTG 
CATATGGATG AGGACATTGT AGTTGTAAAT AAGCCAGCAT CGATACCTGT GCATCCGTGT 
GGACGTTATA GGCACAACAC CGTAGTCTTC ATTCTGGCCA AGGAGTTCAA TCTCAAGAAT 
CTACGCACAA TTCACCGACT AGATCGGTTG ACCTCGGGAC TCCTACTTTT TGGACGCAGT 
CCGAAGAAGG CGCGCCAAAT GGAGCAGCAG ATTCGCAACA GGCAGGTGGA GAAGGAGTAC 
ATCTGCAGGG TGGAGGGCGT TTTTCCAGAT GGCATCGTGG AGTGCAAGGA GCCCATTGAA 
GTAGTTAGCT ACAAGATTGG CGTGTGCAAA GTATCGGCCA AGGGCAAGGA TTGCACGACT 
ACCTTCCAGA AGCTATCCCA AAATGGCACC ACCAGTGTGG TGCTCTGCAA ACCACTCACT 
GGCCGAATGC ATCAGATAAG AGTGCACTTG CAATATTTAG GTTATCCCAT TCTAAACGAT 
CCACTGTACA ATCACGAAGT CTTCGGTCCG CTGAAAGGAC GATCCGGAGA TATCGGTGGC 
AAGTCGGATG AAGAGCTTAT ACGAGATCTC ATAAACATAC ACAATGCAGA GAACTGGCTG 
GGCATAGATT GTGATTCGGA TATATCCATG TTCAAGAGCA CCAAGGATGA GGCAGATCGG 
GAAAGTCTCA GCAGTGAGCA CACTTCCGTT GTTCATCATA GCGATGATGA TGGATGCGTC 
AATTCGCGGG AAACTACACC ACCTTGCAAT GAGCCACAGC AGCCGGAAAA TAGTGTTAAA 
CTTTTAGAAA CTACAAATGC AGTCAAGGAG TATCAAGTTG CGGCACAGAA GTCTTCCTCG 
GAAATTTGCC CAGCTCCACT GGATGCAGTG GAGAGTCCAC TGAATGGAGG TGGATGCAAT 
GTGGGCAAGG TGACAGTAGA TGAGCATTGC TATGAGTGCA AAGTGCACTA CCGTGATCCA 
AAGTCAAAGG ACCTTATAAT GTATCTGCAC GCATGGAAGT ACAAGGGCCC TGGATGGGAG 
TACGAAACTG AGTTACCGAA TTGGGCCCGC AACGATTGGG ACCACTTAGA CTCGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RluA-1 ( NM_164932.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723594.1 Drosophila melanogaster RluA pseudouridine synthase 1 (RluA-1), transcript variant C, mRNA.

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CloneID	OFa14364
Clone ID Related Accession (Same CDS sequence)	NM_164931.2 , NM_164930.2 , NM_164932.2
Accession Version	NM_164932.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2220bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	RluA pseudouridine synthase 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164932.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGAATT CTCCGGCTCC GTGTGCCTGG TACTTGCCCT GGTCCCTGGC CGCCCAGCAG 
CACCAGCAAA AGATGCTGCA AATGCAGTCG CCGTTTCTGG ACAAGATGGG CGCCACATCG 
GTGGGCGGCA TCTTCGCTGG CCAGCCGCAG ATGCAGCAAC AATTGTCGCC CAATACGGCA 
GCAGCACCGC CGGCAAACTA TCAGCAGCCC GCTTTGCATC CAAGCGCCGC ACCAGGCGCA 
CCACACTTCC ACATGGGATC CCCGTATAGC CATCTGGCAC CGCAGCTCCT CAACGCCGGA 
CAGCTGAACC AGAACGCACT GATGCACTCC GCCATGTTCT CTTCCCTGCC ACTTGGTGCG 
TACTATGCAC CCGCCGCCGG CGCAGGTCAC TCGGCCTTTG GTGGCGTTCC CCTGACCACG 
GCTGCCCAGC AATCTCTATT GGCCGCCACC GGAGGAGCAA CTGCTGGCCA TTTGGCCAAC 
CAGCAGACGA CGGCTCAAGT GCCCGTCCAG GTGCCCGTGC AAATGGCCCA ACGGACAGCT 
CCGGCCGCCT GCTCCATGGT CCAGCCACTT AACTGCCTGC CGCACCAGGA ACTGAATCAC 
CTGTCGTCCA TCAATCTCAA CCTGCTGCGC AGTCCGGCGC CTCCGCTCCC AGCCATTCAG 
GTCTTGCCAA GTGCCGAGGT GCCGATTAAT AAGAAGACTA CTTCTCCCAG GGACTCTGAG 
AATCTTAAAA TCAAGGTTCC AGAAAAGCGG AAAACAGACG ATAATGATCA GATAAAAGAG 
CTAAAGAAAG CTAAACTGGA GACAAAAGCA TTGAAGGCTA AGAGGCCGGG ATTCACGGAT 
GAAAGATACC AGGAAACATC CTACTATATG GAAAACGGTC TACGCAAGGT GTATCCGTAC 
TACTTTACAT TCACAACATT TACAAAAGGT CGCTGGGTGG GCGAAAAGAT ATTGGATATA 
TTCTCGCGAG AATTTCGGGC ACATCCCGCT GAGGAATATG AACGTTGTAT CCAAACTGGC 
ACATTGACAG TCAACTTTGA GAAGGTACCC ATTGACTATC GGCTGAAACA TAACGATCTT 
CTCGCCAATA TAGTTCACAG ACACGAAGTT CCTGTGACTT GTCAACCAAT TAAAATTGTG 
CATATGGATG AGGACATTGT AGTTGTAAAT AAGCCAGCAT CGATACCTGT GCATCCGTGT 
GGACGTTATA GGCACAACAC CGTAGTCTTC ATTCTGGCCA AGGAGTTCAA TCTCAAGAAT 
CTACGCACAA TTCACCGACT AGATCGGTTG ACCTCGGGAC TCCTACTTTT TGGACGCAGT 
CCGAAGAAGG CGCGCCAAAT GGAGCAGCAG ATTCGCAACA GGCAGGTGGA GAAGGAGTAC 
ATCTGCAGGG TGGAGGGCGT TTTTCCAGAT GGCATCGTGG AGTGCAAGGA GCCCATTGAA 
GTAGTTAGCT ACAAGATTGG CGTGTGCAAA GTATCGGCCA AGGGCAAGGA TTGCACGACT 
ACCTTCCAGA AGCTATCCCA AAATGGCACC ACCAGTGTGG TGCTCTGCAA ACCACTCACT 
GGCCGAATGC ATCAGATAAG AGTGCACTTG CAATATTTAG GTTATCCCAT TCTAAACGAT 
CCACTGTACA ATCACGAAGT CTTCGGTCCG CTGAAAGGAC GATCCGGAGA TATCGGTGGC 
AAGTCGGATG AAGAGCTTAT ACGAGATCTC ATAAACATAC ACAATGCAGA GAACTGGCTG 
GGCATAGATT GTGATTCGGA TATATCCATG TTCAAGAGCA CCAAGGATGA GGCAGATCGG 
GAAAGTCTCA GCAGTGAGCA CACTTCCGTT GTTCATCATA GCGATGATGA TGGATGCGTC 
AATTCGCGGG AAACTACACC ACCTTGCAAT GAGCCACAGC AGCCGGAAAA TAGTGTTAAA 
CTTTTAGAAA CTACAAATGC AGTCAAGGAG TATCAAGTTG CGGCACAGAA GTCTTCCTCG 
GAAATTTGCC CAGCTCCACT GGATGCAGTG GAGAGTCCAC TGAATGGAGG TGGATGCAAT 
GTGGGCAAGG TGACAGTAGA TGAGCATTGC TATGAGTGCA AAGTGCACTA CCGTGATCCA 
AAGTCAAAGG ACCTTATAAT GTATCTGCAC GCATGGAAGT ACAAGGGCCC TGGATGGGAG 
TACGAAACTG AGTTACCGAA TTGGGCCCGC AACGATTGGG ACCACTTAGA CTCGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723594.1
CDS	224..2443
Translation	MQNSPAPCAWYLPWSLAAQQHQQKMLQMQSPFLDKMGATSVGGIFAGQPQMQQQLSPNTAAAPPANYQQPALHPSAAPGAPHFHMGSPYSHLAPQLLNAGQLNQNALMHSAMFSSLPLGAYYAPAAGAGHSAFGGVPLTTAAQQSLLAATGGATAGHLANQQTTAQVPVQVPVQMAQRTAPAACSMVQPLNCLPHQELNHLSSINLNLLRSPAPPLPAIQVLPSAEVPINKKTTSPRDSENLKIKVPEKRKTDDNDQIKELKKAKLETKALKAKRPGFTDERYQETSYYMENGLRKVYPYYFTFTTFTKGRWVGEKILDIFSREFRAHPAEEYERCIQTGTLTVNFEKVPIDYRLKHNDLLANIVHRHEVPVTCQPIKIVHMDEDIVVVNKPASIPVHPCGRYRHNTVVFILAKEFNLKNLRTIHRLDRLTSGLLLFGRSPKKARQMEQQIRNRQVEKEYICRVEGVFPDGIVECKEPIEVVSYKIGVCKVSAKGKDCTTTFQKLSQNGTTSVVLCKPLTGRMHQIRVHLQYLGYPILNDPLYNHEVFGPLKGRSGDIGGKSDEELIRDLINIHNAENWLGIDCDSDISMFKSTKDEADRESLSSEHTSVVHHSDDDGCVNSRETTPPCNEPQQPENSVKLLETTNAVKEYQVAAQKSSSEICPAPLDAVESPLNGGGCNVGKVTVDEHCYECKVHYRDPKSKDLIMYLHAWKYKGPGWEYETELPNWARNDWDHLDSA

Target ORF information:

RefSeq Version	NM_164932.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster RluA pseudouridine synthase 1 (RluA-1), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164932.2

ORF Insert Sequence:

ATGCAGAATT CTCCGGCTCC GTGTGCCTGG TACTTGCCCT GGTCCCTGGC CGCCCAGCAG 
CACCAGCAAA AGATGCTGCA AATGCAGTCG CCGTTTCTGG ACAAGATGGG CGCCACATCG 
GTGGGCGGCA TCTTCGCTGG CCAGCCGCAG ATGCAGCAAC AATTGTCGCC CAATACGGCA 
GCAGCACCGC CGGCAAACTA TCAGCAGCCC GCTTTGCATC CAAGCGCCGC ACCAGGCGCA 
CCACACTTCC ACATGGGATC CCCGTATAGC CATCTGGCAC CGCAGCTCCT CAACGCCGGA 
CAGCTGAACC AGAACGCACT GATGCACTCC GCCATGTTCT CTTCCCTGCC ACTTGGTGCG 
TACTATGCAC CCGCCGCCGG CGCAGGTCAC TCGGCCTTTG GTGGCGTTCC CCTGACCACG 
GCTGCCCAGC AATCTCTATT GGCCGCCACC GGAGGAGCAA CTGCTGGCCA TTTGGCCAAC 
CAGCAGACGA CGGCTCAAGT GCCCGTCCAG GTGCCCGTGC AAATGGCCCA ACGGACAGCT 
CCGGCCGCCT GCTCCATGGT CCAGCCACTT AACTGCCTGC CGCACCAGGA ACTGAATCAC 
CTGTCGTCCA TCAATCTCAA CCTGCTGCGC AGTCCGGCGC CTCCGCTCCC AGCCATTCAG 
GTCTTGCCAA GTGCCGAGGT GCCGATTAAT AAGAAGACTA CTTCTCCCAG GGACTCTGAG 
AATCTTAAAA TCAAGGTTCC AGAAAAGCGG AAAACAGACG ATAATGATCA GATAAAAGAG 
CTAAAGAAAG CTAAACTGGA GACAAAAGCA TTGAAGGCTA AGAGGCCGGG ATTCACGGAT 
GAAAGATACC AGGAAACATC CTACTATATG GAAAACGGTC TACGCAAGGT GTATCCGTAC 
TACTTTACAT TCACAACATT TACAAAAGGT CGCTGGGTGG GCGAAAAGAT ATTGGATATA 
TTCTCGCGAG AATTTCGGGC ACATCCCGCT GAGGAATATG AACGTTGTAT CCAAACTGGC 
ACATTGACAG TCAACTTTGA GAAGGTACCC ATTGACTATC GGCTGAAACA TAACGATCTT 
CTCGCCAATA TAGTTCACAG ACACGAAGTT CCTGTGACTT GTCAACCAAT TAAAATTGTG 
CATATGGATG AGGACATTGT AGTTGTAAAT AAGCCAGCAT CGATACCTGT GCATCCGTGT 
GGACGTTATA GGCACAACAC CGTAGTCTTC ATTCTGGCCA AGGAGTTCAA TCTCAAGAAT 
CTACGCACAA TTCACCGACT AGATCGGTTG ACCTCGGGAC TCCTACTTTT TGGACGCAGT 
CCGAAGAAGG CGCGCCAAAT GGAGCAGCAG ATTCGCAACA GGCAGGTGGA GAAGGAGTAC 
ATCTGCAGGG TGGAGGGCGT TTTTCCAGAT GGCATCGTGG AGTGCAAGGA GCCCATTGAA 
GTAGTTAGCT ACAAGATTGG CGTGTGCAAA GTATCGGCCA AGGGCAAGGA TTGCACGACT 
ACCTTCCAGA AGCTATCCCA AAATGGCACC ACCAGTGTGG TGCTCTGCAA ACCACTCACT 
GGCCGAATGC ATCAGATAAG AGTGCACTTG CAATATTTAG GTTATCCCAT TCTAAACGAT 
CCACTGTACA ATCACGAAGT CTTCGGTCCG CTGAAAGGAC GATCCGGAGA TATCGGTGGC 
AAGTCGGATG AAGAGCTTAT ACGAGATCTC ATAAACATAC ACAATGCAGA GAACTGGCTG 
GGCATAGATT GTGATTCGGA TATATCCATG TTCAAGAGCA CCAAGGATGA GGCAGATCGG 
GAAAGTCTCA GCAGTGAGCA CACTTCCGTT GTTCATCATA GCGATGATGA TGGATGCGTC 
AATTCGCGGG AAACTACACC ACCTTGCAAT GAGCCACAGC AGCCGGAAAA TAGTGTTAAA 
CTTTTAGAAA CTACAAATGC AGTCAAGGAG TATCAAGTTG CGGCACAGAA GTCTTCCTCG 
GAAATTTGCC CAGCTCCACT GGATGCAGTG GAGAGTCCAC TGAATGGAGG TGGATGCAAT 
GTGGGCAAGG TGACAGTAGA TGAGCATTGC TATGAGTGCA AAGTGCACTA CCGTGATCCA 
AAGTCAAAGG ACCTTATAAT GTATCTGCAC GCATGGAAGT ACAAGGGCCC TGGATGGGAG 
TACGAAACTG AGTTACCGAA TTGGGCCCGC AACGATTGGG ACCACTTAGA CTCGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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RluA-1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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