trk cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	trk
Entrez Gene ID	34376
Full Name	trunk
Synonyms	CG5619,Dmel\CG5619,Trk,fs(2)trunk
General protein information	Preferred Names trunk Names CG5619 gene product from transcript CG5619-RA CG5619-PA trk-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 31B1-31B1\|2-36 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_057419.3	NP_476767.2	trunk

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002846	XP_563293.4
Drosophila melanogaster (fruit fly)	trk	NP_476767.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Torso interacts with both Trunk and Torso-like, which cooperate to mediate dimerization and activation of Torso at the ends of the Drosophila embryo
Title: Reconstitution of Torso signaling in cultured cells suggests a role for both Trunk and Torso-like in receptor activation.

Present and other previous studies, suggest that the function of Tsl is to mediate secretion of Trk, thus providing the mechanism for spatially restricted activation of Tor in Drosophila embryos.
Title: Torso-like mediates extracellular accumulation of Furin-cleaved Trunk to pattern the Drosophila embryo termini.

Data favours a scenario where Tsl functions post proteolytic processing of Trk to control localized terminal patterning.
Title: Trunk cleavage is essential for Drosophila terminal patterning and can occur independently of Torso-like.

Extracellular processing of the Trunk ligand at the anterior and posterior termini of the developing embryo activates the signaling pathway that defines the two ends.
Title: The torso ligand, unmasked?

The following trk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the trk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14303	NM_057419.3 Latest version!	Drosophila melanogaster trunk (trk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

trk ( NM_057419.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476767.2 Drosophila melanogaster trunk (trk), mRNA.

Price & Availability↑

CloneID	OFa14303
Clone ID Related Accession (Same CDS sequence)	NM_057419.3
Accession Version	NM_057419.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	trunk
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057419.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTCTGC GTATACTGTG TCCAACTATG AAATCCCAAA GTGAACTGGC CATTGTGCTC 
ACTTGGTTGG CAGTGCTGGG GACGGCTCAA GATGATGCCG ATTACTGCGC CGAGCTATCC 
ACGCAGTCGC TGGCCAAAAT TCTGGGACAG GCATTTAATC CGCGCTACAT GAGCATCGAT 
CCGCCGGGCG AGCCCGAGGA GAAGAGCTAT CATCTGGGAT ACAAGCGATC GTCGTACGAG 
CTGCCCTTCT ATGCGGACAG TGATGCGATT TCGGTTAGCC ACTTTCCCGC CTGGGAGACC 
AACCACTTTG CGCTGGTAGA GAAGAAGAAG GAGGCACCGA GGAGCAAGAG TCTGCGCACG 
AGAAGCGCCT TCATGGATCG TGTGGGACAT CCGAGAATCG ATGGGTTCAA GCAGCGGCCG 
TGGGAGTGCT CGTCGAAGAT CAACTGGATT GACTTAGGCT TAAATTACTT TCCACGCTAT 
ATCCGTTCGA TCGAGTGTAT TGCCAGAAAA TGTTGGTATG ATCACTTTAA TTGCAAGCCA 
AAATCATTTA CAATAAAAGT ACTGCGCCGT AAAACTGGCT CATGCATTCG AATAAACGAT 
AAATTGATTT TGATCACCGC AGAGAAGTTT GAGAATGATT ATACGCAGCT CTGGATTTGG 
GAAGAAATAG CGGTTAACTT TTGCTGTGAA TGTGTTATGC TATACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476767.2
CDS	116..823
Translation	MFLRILCPTMKSQSELAIVLTWLAVLGTAQDDADYCAELSTQSLAKILGQAFNPRYMSIDPPGEPEEKSYHLGYKRSSYELPFYADSDAISVSHFPAWETNHFALVEKKKEAPRSKSLRTRSAFMDRVGHPRIDGFKQRPWECSSKINWIDLGLNYFPRYIRSIECIARKCWYDHFNCKPKSFTIKVLRRKTGSCIRINDKLILITAEKFENDYTQLWIWEEIAVNFCCECVMLY

Target ORF information:

RefSeq Version	NM_057419.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster trunk (trk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057419.3

ORF Insert Sequence:

ATGTTTCTGC GTATACTGTG TCCAACTATG AAATCCCAAA GTGAACTGGC CATTGTGCTC 
ACTTGGTTGG CAGTGCTGGG GACGGCTCAA GATGATGCCG ATTACTGCGC CGAGCTATCC 
ACGCAGTCGC TGGCCAAAAT TCTGGGACAG GCATTTAATC CGCGCTACAT GAGCATCGAT 
CCGCCGGGCG AGCCCGAGGA GAAGAGCTAT CATCTGGGAT ACAAGCGATC GTCGTACGAG 
CTGCCCTTCT ATGCGGACAG TGATGCGATT TCGGTTAGCC ACTTTCCCGC CTGGGAGACC 
AACCACTTTG CGCTGGTAGA GAAGAAGAAG GAGGCACCGA GGAGCAAGAG TCTGCGCACG 
AGAAGCGCCT TCATGGATCG TGTGGGACAT CCGAGAATCG ATGGGTTCAA GCAGCGGCCG 
TGGGAGTGCT CGTCGAAGAT CAACTGGATT GACTTAGGCT TAAATTACTT TCCACGCTAT 
ATCCGTTCGA TCGAGTGTAT TGCCAGAAAA TGTTGGTATG ATCACTTTAA TTGCAAGCCA 
AAATCATTTA CAATAAAAGT ACTGCGCCGT AAAACTGGCT CATGCATTCG AATAAACGAT 
AAATTGATTT TGATCACCGC AGAGAAGTTT GAGAATGATT ATACGCAGCT CTGGATTTGG 
GAAGAAATAG CGGTTAACTT TTGCTGTGAA TGTGTTATGC TATACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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trk cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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