nAChRalpha6 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	nAChRalpha6
Entrez Gene ID	34304
Full Name	nicotinic Acetylcholine Receptor alpha6
Synonyms	BcDNA:GH01410,CG4128,CT13662,Dalpha6,Dmel\CG4128,alpha6,das,nAChR,nAChR alpha 6,nAChR-alpha30D,nAChRAlpha-30D,nAChRalpha,nAChRalpha-30D,nAcRalpha-30D,rsn
General protein information	Preferred Names nicotinic Acetylcholine Receptor alpha6 Names CG4128 gene product from transcript CG4128-RC CG4128-PA CG4128-PB CG4128-PC CG4128-PD CG4128-PE CG4128-PG CG4128-PH Dalpha6 alpha6 nicotinic acetylcholine receptor subunit nAChRalpha6-PA nAChRalpha6-PB nAChRalpha6-PC nAChRalpha6-PD nAChRalpha6-PE nAChRalpha6-PG nAChRalpha6-PH nicotinic acetylcholine receptor 30D nicotinic acetylcholine receptor alpha 30D nicotinic acetylcholine receptor alpha6 resistance to spinosyn resistant to spinosyns
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 30D1-30E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001273373.2	NP_001260302.1	nicotinic acetylcholine receptor alpha6, isoform H
NM_205951.2	NP_995673.1	nicotinic acetylcholine receptor alpha6, isoform B
NM_164874.3	NP_723494.2	nicotinic acetylcholine receptor alpha6, isoform A
NM_135472.6	NP_609316.4	nicotinic acetylcholine receptor alpha6, isoform C
NM_205952.3	NP_995674.1	nicotinic acetylcholine receptor alpha6, isoform D
NM_205953.3	NP_995675.1	nicotinic acetylcholine receptor alpha6, isoform E
NM_001201835.3	NP_001188764.1	nicotinic acetylcholine receptor alpha6, isoform G

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	nAcRalpha-30D	NP_001188764.1
Caenorhabditis elegans (roundworm)	acr-19	NP_492429.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002152	XP_308042.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The introduced G275E mutation of the nAChR alpha6 subunit confers strong resistance in Drosophila to spinosad, with the LC50 increasing from around 5 mg L-1 to 335 mg L-1.
Title: A CRISPR/Cas9 mediated point mutation in the alpha 6 subunit of the nicotinic acetylcholine receptor confers resistance to spinosad in Drosophila melanogaster.

In addition to the formation of homomeric nAChR complexes, evidence has been obtained from both radioligand binding and co-immunoprecipitation studies for the co-assembly of Dalpha6 and Dalpha7 into heteromeric cell surface complexes
Title: Molecular characterization of Dalpha6 and Dalpha7 nicotinic acetylcholine receptor subunits from Drosophila: formation of a high-affinity alpha-bungarotoxin binding site revealed by expression of subunit chimeras.

The following nAChRalpha6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nAChRalpha6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa14233	NM_135472.6 Latest version!	Drosophila melanogaster nicotinic acetylcholine receptor alpha6, transcript variant C (nAChRalpha6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa14234	NM_205953.3 Latest version!	Drosophila melanogaster nicotinic acetylcholine receptor alpha6, transcript variant E (nAChRalpha6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa14235	NM_205952.3 Latest version!	Drosophila melanogaster nicotinic acetylcholine receptor alpha6, transcript variant D (nAChRalpha6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa14236	NM_205951.2 Latest version!	Drosophila melanogaster nicotinic acetylcholine receptor alpha6, transcript variant B (nAChRalpha6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa14236	NM_001201835.3 Latest version!	Drosophila melanogaster nicotinic acetylcholine receptor alpha6, transcript variant G (nAChRalpha6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa14237	NM_164874.3 Latest version!	Drosophila melanogaster nicotinic acetylcholine receptor alpha6, transcript variant A (nAChRalpha6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa15963	NM_001273373.2 Latest version!	Drosophila melanogaster nicotinic acetylcholine receptor alpha6, transcript variant H (nAChRalpha6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nAChRalpha6 ( NM_135472.6 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609316.4 Drosophila melanogaster nicotinic acetylcholine receptor alpha6, transcript variant C (nAChRalpha6), mRNA.

Price & Availability↑

CloneID	OFa14233
Clone ID Related Accession (Same CDS sequence)	NM_135472.6
Accession Version	NM_135472.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nicotinic acetylcholine receptor alpha6, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_135472.5. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACTCCC CGCTGCCAGC GTCGCTGTCG CTGTTTGTCC TGTTGATCTT TCTGGCGATA 
ATTAAAGAAA GCTGTCAAGG ACCTCATGAA AAGCGCCTGC TGAACCATCT GCTGTCCACC 
TACAATACGC TGGAGCGACC CGTGGCCAAT GAATCGGAGC CCCTGGAGGT TAAGTTCGGA 
CTGACGCTGC AGCAGATCAT CGACGTGGAC GAGAAGAATC AGCTTCTCAT AACGAATCTT 
TGGCTTTCGT TGGAGTGGAA CGACTACAAT CTGCGCTGGA ATGAAACGGA ATACGGCGGG 
GTCAAGGATC TACGAATCAC GCCCAACAAG CTGTGGAAGC CCGACGTGCT CATGTACAAC 
AGCGCGGATG AGGGATTCGA TGGCACGTAT CACACCAACA TTGTGGTCAA ACATAACGGC 
AGTTGTCTGT ACGTGCCCCC TGGTATCTTC AAGAGCACAT GCAAGATAGA CATCACGTGG 
TTCCCATTTG ATGACCAACA TTGCGAAATG AAATTCGGTA GTTGGACTTA CGATGGAAAT 
CAGTTGGATT TGGTTTTGAA TTCCGAAGAT GGAGGGGATC TTTCCGATTT CATAACAAAT 
GGCGAGTGGT ACTTGCTTGC CATGCCGGGA AAGAAGAATA CGATAGTCTA CGCCTGCTGC 
CCAGAACCAT ATGTCGATAT CACCTTTACT ATACAAATTC GTCGCCGTAC ATTATATTAT 
TTTTTCAATT TAATTGTGCC ATGTGTGCTA ATCTCATCGA TGGCCCTACT GGGCTTCACA 
TTGCCGCCGG ATTCGGGCGA GAAACTGACG CTGGGAGTTA CAATTCTTCT ATCGCTCACA 
GTGTTTCTCA ACCTTGTAGC TGAGACATTG CCCCAAGTAT CTGATGCAAT CCCCTTGTTA 
GGCACCTACT TCAATTGCAT CATGTTCATG GTCGCCTCGT CGGTGGTGCT GACAGTAGTG 
GTGCTCAACT ACCACCATCG CACAGCGGAC ATTCACGAGA TGCCACCGTG GATCAAGTCC 
GTTTTCCTAC AATGGCTGCC CTGGATCTTG CGAATGGGTC GACCCGGTCG CAAGATTACA 
CGCAAAACAA TACTATTAAG CAATCGCATG AAGGAGCTGG AGCTAAAGGA GCGCTCCTCC 
AAATCCCTGC TGGCCAATGT CCTCGACATC GACGACGACT TCCGGCACAC AATATCTGGC 
TCCCAAACCG CCATTGGCTC GTCGGCCAGC TTCGGTCGGC CCACAACGGT GGAGGAGCAT 
CACACGGCCA TCGGCTGCAA TCACAAAGAT CTTCATCTAA TTCTCAAAGA ATTGCAATTT 
ATTACGGCGC GGATGCGCAA AGCTGACGAC GAAGCGGAAT TGATCGGCGA TTGGAAGTTC 
GCGGCAATGG TTGTGGATAG ATTTTGTTTA ATTGTTTTCA CGCTCTTCAC GATTATTGCA 
ACGGTTACGG TGCTGCTCTC CGCTCCGCAC ATAATCGTGC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609316.4
CDS	1448..2932
Translation	MDSPLPASLSLFVLLIFLAIIKESCQGPHEKRLLNHLLSTYNTLERPVANESEPLEVKFGLTLQQIIDVDEKNQLLITNLWLSLEWNDYNLRWNETEYGGVKDLRITPNKLWKPDVLMYNSADEGFDGTYHTNIVVKHNGSCLYVPPGIFKSTCKIDITWFPFDDQHCEMKFGSWTYDGNQLDLVLNSEDGGDLSDFITNGEWYLLAMPGKKNTIVYACCPEPYVDITFTIQIRRRTLYYFFNLIVPCVLISSMALLGFTLPPDSGEKLTLGVTILLSLTVFLNLVAETLPQVSDAIPLLGTYFNCIMFMVASSVVLTVVVLNYHHRTADIHEMPPWIKSVFLQWLPWILRMGRPGRKITRKTILLSNRMKELELKERSSKSLLANVLDIDDDFRHTISGSQTAIGSSASFGRPTTVEEHHTAIGCNHKDLHLILKELQFITARMRKADDEAELIGDWKFAAMVVDRFCLIVFTLFTIIATVTVLLSAPHIIVQ

Target ORF information:

RefSeq Version	NM_135472.6
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nicotinic acetylcholine receptor alpha6, transcript variant C (nAChRalpha6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135472.6

ORF Insert Sequence:

ATGGACTCCC CGCTGCCAGC GTCGCTGTCG CTGTTTGTCC TGTTGATCTT TCTGGCGATA 
ATTAAAGAAA GCTGTCAAGG ACCTCATGAA AAGCGCCTGC TGAACCATCT GCTGTCCACC 
TACAATACGC TGGAGCGACC CGTGGCCAAT GAATCGGAGC CCCTGGAGGT TAAGTTCGGA 
CTGACGCTGC AGCAGATCAT CGACGTGGAC GAGAAGAATC AGCTTCTCAT AACGAATCTT 
TGGCTTTCGT TGGAGTGGAA CGACTACAAT CTGCGCTGGA ATGAAACGGA ATACGGCGGG 
GTCAAGGATC TACGAATCAC GCCCAACAAG CTGTGGAAGC CCGACGTGCT CATGTACAAC 
AGCGCGGATG AGGGATTCGA TGGCACGTAT CACACCAACA TTGTGGTCAA ACATAACGGC 
AGTTGTCTGT ACGTGCCCCC TGGTATCTTC AAGAGCACAT GCAAGATAGA CATCACGTGG 
TTCCCATTTG ATGACCAACA TTGCGAAATG AAATTCGGTA GTTGGACTTA CGATGGAAAT 
CAGTTGGATT TGGTTTTGAA TTCCGAAGAT GGAGGGGATC TTTCCGATTT CATAACAAAT 
GGCGAGTGGT ACTTGCTTGC CATGCCGGGA AAGAAGAATA CGATAGTCTA CGCCTGCTGC 
CCAGAACCAT ATGTCGATAT CACCTTTACT ATACAAATTC GTCGCCGTAC ATTATATTAT 
TTTTTCAATT TAATTGTGCC ATGTGTGCTA ATCTCATCGA TGGCCCTACT GGGCTTCACA 
TTGCCGCCGG ATTCGGGCGA GAAACTGACG CTGGGAGTTA CAATTCTTCT ATCGCTCACA 
GTGTTTCTCA ACCTTGTAGC TGAGACATTG CCCCAAGTAT CTGATGCAAT CCCCTTGTTA 
GGCACCTACT TCAATTGCAT CATGTTCATG GTCGCCTCGT CGGTGGTGCT GACAGTAGTG 
GTGCTCAACT ACCACCATCG CACAGCGGAC ATTCACGAGA TGCCACCGTG GATCAAGTCC 
GTTTTCCTAC AATGGCTGCC CTGGATCTTG CGAATGGGTC GACCCGGTCG CAAGATTACA 
CGCAAAACAA TACTATTAAG CAATCGCATG AAGGAGCTGG AGCTAAAGGA GCGCTCCTCC 
AAATCCCTGC TGGCCAATGT CCTCGACATC GACGACGACT TCCGGCACAC AATATCTGGC 
TCCCAAACCG CCATTGGCTC GTCGGCCAGC TTCGGTCGGC CCACAACGGT GGAGGAGCAT 
CACACGGCCA TCGGCTGCAA TCACAAAGAT CTTCATCTAA TTCTCAAAGA ATTGCAATTT 
ATTACGGCGC GGATGCGCAA AGCTGACGAC GAAGCGGAAT TGATCGGCGA TTGGAAGTTC 
GCGGCAATGG TTGTGGATAG ATTTTGTTTA ATTGTTTTCA CGCTCTTCAC GATTATTGCA 
ACGGTTACGG TGCTGCTCTC CGCTCCGCAC ATAATCGTGC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nAChRalpha6 ( NM_205953.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995675.1 Drosophila melanogaster nicotinic acetylcholine receptor alpha6, transcript variant E (nAChRalpha6), mRNA.

Price & Availability↑

CloneID	OFa14234
Clone ID Related Accession (Same CDS sequence)	NM_205953.3
Accession Version	NM_205953.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nicotinic acetylcholine receptor alpha6, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_205953.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACTCCC CGCTGCCAGC GTCGCTGTCG CTGTTTGTCC TGTTGATCTT TCTGGCGATA 
ATTAAAGAAA GCTGTCAAGG ACCTCATGAA AAGCGCCTGC TGAACCATCT GCTGTCCACC 
TACAATACGC TGGAGCGACC CGTGGCCAAT GAATCGGAGC CCCTGGAGGT TAAGTTCGGA 
CTGACGCTGC AGCAGATCAT CGACGTGGAT GAAAAGAATC AGATTCTGAC CACAAATGCG 
TGGTTAAATT TGGAGTGGAA CGACTACAAT CTGCGCTGGA ATGAAACGGA ATACGGCGGG 
GTCAAGGATC TACGAATCAC GCCCAACAAG CTGTGGAAGC CCGACGTGCT CATGTACAAC 
AGCGCGGATG AGGGATTCGA TGGCACGTAT CACACCAACA TTGTGGTCAA ACATAACGGC 
AGTTGTCTGT ACGTGCCCCC TGGTATCTTC AAGAGCACAT GCAAGATAGA CATCACGTGG 
TTCCCATTTG ATGACCAACA TTGCGAAATG AAATTCGGTA GTTGGACTTA CGATGGAAAT 
CAGTTGGATT TGGTTTTGAA TTCCGAAGAT GGAGGGGATC TTTCCGATTT CATAACAAAT 
GGCGAGTGGT ACTTGCTTGC CATGCCGGGA AAGAAGAATA CGATAGTCTA CGCCTGCTGC 
CCAGAACCAT ATGTCGATAT CACCTTTACT ATACAAATTC GTCGCCGTAC ATTATATTAT 
TTTTTCAATT TAATTGTGCC ATGTGTGCTA ATCTCATCGA TGGCCCTACT GGGCTTCACA 
TTGCCGCCGG ATTCGGGCGA GAAACTGACG CTGGGCGTAA CTATACTACT ATCATTAACA 
GTATTTCTAA ACCTTGTCGC CGAGTCCATG CCGACAACGT CGGATGCTGT TCCTCTTATA 
GGAGTTACAA TTCTTCTATC GCTCACAGTG TTTCTCAACC TTGTAGCTGA GACATTGCCC 
CAAGTATCTG ATGCAATCCC CTTGTTAGGC ACCTACTTCA ATTGCATCAT GTTCATGGTC 
GCCTCGTCGG TGGTGCTGAC AGTAGTGGTG CTCAACTACC ACCATCGCAC AGCGGACATT 
CACGAGATGC CACCGTGGAT CAAGTCCGTT TTCCTACAAT GGCTGCCCTG GATCTTGCGA 
ATGGGTCGAC CCGGTCGCAA GATTACACGC AAAACAATAC TATTAAGCAA TCGCATGAAG 
GAGCTGGAGC TAAAGGAGCG CTCCTCCAAA TCCCTGCTGG CCAATGTCCT CGACATCGAC 
GACGACTTCC GGCACACAAT ATCTGGCTCC CAAACCGCCA TTGGCTCGTC GGCCAGCTTC 
GGTCGGCCCA CAACGGTGGA GGAGCATCAC ACGGCCATCG GCTGCAATCA CAAAGATCTT 
CATCTAATTC TCAAAGAATT GCAATTTATT ACGGCGCGGA TGCGCAAAGC TGACGACGAA 
GCGGAATTGA TCGGCGATTG GAAGTTCGCG GCAATGGTTG TGGATAGATT TTGTTTAATT 
GTTTTCACGC TCTTCACGAT TATTGCAACG GTTACGGTGC TGCTCTCCGC TCCGCACATA 
ATCGTGCAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995675.1
CDS	1242..2813
Translation	MDSPLPASLSLFVLLIFLAIIKESCQGPHEKRLLNHLLSTYNTLERPVANESEPLEVKFGLTLQQIIDVDEKNQILTTNAWLNLEWNDYNLRWNETEYGGVKDLRITPNKLWKPDVLMYNSADEGFDGTYHTNIVVKHNGSCLYVPPGIFKSTCKIDITWFPFDDQHCEMKFGSWTYDGNQLDLVLNSEDGGDLSDFITNGEWYLLAMPGKKNTIVYACCPEPYVDITFTIQIRRRTLYYFFNLIVPCVLISSMALLGFTLPPDSGEKLTLGVTILLSLTVFLNLVAESMPTTSDAVPLIGVTILLSLTVFLNLVAETLPQVSDAIPLLGTYFNCIMFMVASSVVLTVVVLNYHHRTADIHEMPPWIKSVFLQWLPWILRMGRPGRKITRKTILLSNRMKELELKERSSKSLLANVLDIDDDFRHTISGSQTAIGSSASFGRPTTVEEHHTAIGCNHKDLHLILKELQFITARMRKADDEAELIGDWKFAAMVVDRFCLIVFTLFTIIATVTVLLSAPHIIVQ

Target ORF information:

RefSeq Version	NM_205953.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nicotinic acetylcholine receptor alpha6, transcript variant E (nAChRalpha6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205953.3

ORF Insert Sequence:

ATGGACTCCC CGCTGCCAGC GTCGCTGTCG CTGTTTGTCC TGTTGATCTT TCTGGCGATA 
ATTAAAGAAA GCTGTCAAGG ACCTCATGAA AAGCGCCTGC TGAACCATCT GCTGTCCACC 
TACAATACGC TGGAGCGACC CGTGGCCAAT GAATCGGAGC CCCTGGAGGT TAAGTTCGGA 
CTGACGCTGC AGCAGATCAT CGACGTGGAT GAAAAGAATC AGATTCTGAC CACAAATGCG 
TGGTTAAATT TGGAGTGGAA CGACTACAAT CTGCGCTGGA ATGAAACGGA ATACGGCGGG 
GTCAAGGATC TACGAATCAC GCCCAACAAG CTGTGGAAGC CCGACGTGCT CATGTACAAC 
AGCGCGGATG AGGGATTCGA TGGCACGTAT CACACCAACA TTGTGGTCAA ACATAACGGC 
AGTTGTCTGT ACGTGCCCCC TGGTATCTTC AAGAGCACAT GCAAGATAGA CATCACGTGG 
TTCCCATTTG ATGACCAACA TTGCGAAATG AAATTCGGTA GTTGGACTTA CGATGGAAAT 
CAGTTGGATT TGGTTTTGAA TTCCGAAGAT GGAGGGGATC TTTCCGATTT CATAACAAAT 
GGCGAGTGGT ACTTGCTTGC CATGCCGGGA AAGAAGAATA CGATAGTCTA CGCCTGCTGC 
CCAGAACCAT ATGTCGATAT CACCTTTACT ATACAAATTC GTCGCCGTAC ATTATATTAT 
TTTTTCAATT TAATTGTGCC ATGTGTGCTA ATCTCATCGA TGGCCCTACT GGGCTTCACA 
TTGCCGCCGG ATTCGGGCGA GAAACTGACG CTGGGCGTAA CTATACTACT ATCATTAACA 
GTATTTCTAA ACCTTGTCGC CGAGTCCATG CCGACAACGT CGGATGCTGT TCCTCTTATA 
GGAGTTACAA TTCTTCTATC GCTCACAGTG TTTCTCAACC TTGTAGCTGA GACATTGCCC 
CAAGTATCTG ATGCAATCCC CTTGTTAGGC ACCTACTTCA ATTGCATCAT GTTCATGGTC 
GCCTCGTCGG TGGTGCTGAC AGTAGTGGTG CTCAACTACC ACCATCGCAC AGCGGACATT 
CACGAGATGC CACCGTGGAT CAAGTCCGTT TTCCTACAAT GGCTGCCCTG GATCTTGCGA 
ATGGGTCGAC CCGGTCGCAA GATTACACGC AAAACAATAC TATTAAGCAA TCGCATGAAG 
GAGCTGGAGC TAAAGGAGCG CTCCTCCAAA TCCCTGCTGG CCAATGTCCT CGACATCGAC 
GACGACTTCC GGCACACAAT ATCTGGCTCC CAAACCGCCA TTGGCTCGTC GGCCAGCTTC 
GGTCGGCCCA CAACGGTGGA GGAGCATCAC ACGGCCATCG GCTGCAATCA CAAAGATCTT 
CATCTAATTC TCAAAGAATT GCAATTTATT ACGGCGCGGA TGCGCAAAGC TGACGACGAA 
GCGGAATTGA TCGGCGATTG GAAGTTCGCG GCAATGGTTG TGGATAGATT TTGTTTAATT 
GTTTTCACGC TCTTCACGAT TATTGCAACG GTTACGGTGC TGCTCTCCGC TCCGCACATA 
ATCGTGCAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nAChRalpha6 ( NM_205952.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995674.1 Drosophila melanogaster nicotinic acetylcholine receptor alpha6, transcript variant D (nAChRalpha6), mRNA.

Price & Availability↑

CloneID	OFa14235
Clone ID Related Accession (Same CDS sequence)	NM_205952.3
Accession Version	NM_205952.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nicotinic acetylcholine receptor alpha6, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_205952.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACTCCC CGCTGCCAGC GTCGCTGTCG CTGTTTGTCC TGTTGATCTT TCTGGCGATA 
ATTAAAGAAA GCTGTCAAGG ACCTCATGAA AAGCGCCTGC TGAACCATCT GCTGTCCACC 
TACAATACGC TGGAGCGACC CGTGGCCAAT GAATCGGAGC CCCTGGAGGT TAAGTTCGGA 
CTGACGCTGC AGCAGATCAT CGACGTGGAT GAAAAGAATC AGATTCTGAC CACAAATGCG 
TGGTTAAATT TGGACGAGAA GAATCAGCTT CTCATAACGA ATCTTTGGCT TTCGTTGGAG 
TGGAACGACT ACAATCTGCG CTGGAATGAA ACGGAATACG GCGGGGTCAA GGATCTACGA 
ATCACGCCCA ACAAGCTGTG GAAGCCCGAC GTGCTCATGT ACAACAGCGC GGATGAGGGA 
TTCGATGGCA CGTATCACAC CAACATTGTG GTCAAACATA ACGGCAGTTG TCTGTACGTG 
CCCCCTGGTA TCTTCAAGAG CACATGCAAG ATAGACATCA CGTGGTTCCC ATTTGATGAC 
CAACATTGCG AAATGAAATT CGGTAGTTGG ACTTACGATG GAAATCAGTT GGATTTGGTT 
TTGAATTCCG AAGATGGAGG GGATCTTTCC GATTTCATAA CAAATGGCGA GTGGTACTTG 
CTTGCCATGC CGGGAAAGAA GAATACGATA GTCTACGCCT GCTGCCCAGA ACCATATGTC 
GATATCACCT TTACTATACA AATTCGTCGC CGTACATTAT ATTATTTTTT CAATTTAATT 
GTGCCATGTG TGCTAATCTC ATCGATGGCC CTACTGGGCT TCACATTGCC GCCGGATTCG 
GGCGAGAAAC TGACGCTGGG AGTTACAATT CTTCTATCGC TCACAGTGTT TCTCAACCTT 
GTAGCTGAGA CATTGCCCCA AGTATCTGAT GCAATCCCCT TGTTAGGCAC CTACTTCAAT 
TGCATCATGT TCATGGTCGC CTCGTCGGTG GTGCTGACAG TAGTGGTGCT CAACTACCAC 
CATCGCACAG CGGACATTCA CGAGATGCCA CCGTGGATCA AGTCCGTTTT CCTACAATGG 
CTGCCCTGGA TCTTGCGAAT GGGTCGACCC GGTCGCAAGA TTACACGCAA AACAATACTA 
TTAAGCAATC GCATGAAGGA GCTGGAGCTA AAGGAGCGCT CCTCCAAATC CCTGCTGGCC 
AATGTCCTCG ACATCGACGA CGACTTCCGG CACACAATAT CTGGCTCCCA AACCGCCATT 
GGCTCGTCGG CCAGCTTCGG TCGGCCCACA ACGGTGGAGG AGCATCACAC GGCCATCGGC 
TGCAATCACA AAGATCTTCA TCTAATTCTC AAAGAATTGC AATTTATTAC GGCGCGGATG 
CGCAAAGCTG ACGACGAAGC GGAATTGATC GGCGATTGGA AGTTCGCGGC AATGGTTGTG 
GATAGATTTT GTTTAATTGT TTTCACGCTC TTCACGATTA TTGCAACGGT TACGGTGCTG 
CTCTCCGCTC CGCACATAAT CGTGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995674.1
CDS	2372..3901
Translation	MDSPLPASLSLFVLLIFLAIIKESCQGPHEKRLLNHLLSTYNTLERPVANESEPLEVKFGLTLQQIIDVDEKNQILTTNAWLNLDEKNQLLITNLWLSLEWNDYNLRWNETEYGGVKDLRITPNKLWKPDVLMYNSADEGFDGTYHTNIVVKHNGSCLYVPPGIFKSTCKIDITWFPFDDQHCEMKFGSWTYDGNQLDLVLNSEDGGDLSDFITNGEWYLLAMPGKKNTIVYACCPEPYVDITFTIQIRRRTLYYFFNLIVPCVLISSMALLGFTLPPDSGEKLTLGVTILLSLTVFLNLVAETLPQVSDAIPLLGTYFNCIMFMVASSVVLTVVVLNYHHRTADIHEMPPWIKSVFLQWLPWILRMGRPGRKITRKTILLSNRMKELELKERSSKSLLANVLDIDDDFRHTISGSQTAIGSSASFGRPTTVEEHHTAIGCNHKDLHLILKELQFITARMRKADDEAELIGDWKFAAMVVDRFCLIVFTLFTIIATVTVLLSAPHIIVQ

Target ORF information:

RefSeq Version	NM_205952.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nicotinic acetylcholine receptor alpha6, transcript variant D (nAChRalpha6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205952.3

ORF Insert Sequence:

ATGGACTCCC CGCTGCCAGC GTCGCTGTCG CTGTTTGTCC TGTTGATCTT TCTGGCGATA 
ATTAAAGAAA GCTGTCAAGG ACCTCATGAA AAGCGCCTGC TGAACCATCT GCTGTCCACC 
TACAATACGC TGGAGCGACC CGTGGCCAAT GAATCGGAGC CCCTGGAGGT TAAGTTCGGA 
CTGACGCTGC AGCAGATCAT CGACGTGGAT GAAAAGAATC AGATTCTGAC CACAAATGCG 
TGGTTAAATT TGGACGAGAA GAATCAGCTT CTCATAACGA ATCTTTGGCT TTCGTTGGAG 
TGGAACGACT ACAATCTGCG CTGGAATGAA ACGGAATACG GCGGGGTCAA GGATCTACGA 
ATCACGCCCA ACAAGCTGTG GAAGCCCGAC GTGCTCATGT ACAACAGCGC GGATGAGGGA 
TTCGATGGCA CGTATCACAC CAACATTGTG GTCAAACATA ACGGCAGTTG TCTGTACGTG 
CCCCCTGGTA TCTTCAAGAG CACATGCAAG ATAGACATCA CGTGGTTCCC ATTTGATGAC 
CAACATTGCG AAATGAAATT CGGTAGTTGG ACTTACGATG GAAATCAGTT GGATTTGGTT 
TTGAATTCCG AAGATGGAGG GGATCTTTCC GATTTCATAA CAAATGGCGA GTGGTACTTG 
CTTGCCATGC CGGGAAAGAA GAATACGATA GTCTACGCCT GCTGCCCAGA ACCATATGTC 
GATATCACCT TTACTATACA AATTCGTCGC CGTACATTAT ATTATTTTTT CAATTTAATT 
GTGCCATGTG TGCTAATCTC ATCGATGGCC CTACTGGGCT TCACATTGCC GCCGGATTCG 
GGCGAGAAAC TGACGCTGGG AGTTACAATT CTTCTATCGC TCACAGTGTT TCTCAACCTT 
GTAGCTGAGA CATTGCCCCA AGTATCTGAT GCAATCCCCT TGTTAGGCAC CTACTTCAAT 
TGCATCATGT TCATGGTCGC CTCGTCGGTG GTGCTGACAG TAGTGGTGCT CAACTACCAC 
CATCGCACAG CGGACATTCA CGAGATGCCA CCGTGGATCA AGTCCGTTTT CCTACAATGG 
CTGCCCTGGA TCTTGCGAAT GGGTCGACCC GGTCGCAAGA TTACACGCAA AACAATACTA 
TTAAGCAATC GCATGAAGGA GCTGGAGCTA AAGGAGCGCT CCTCCAAATC CCTGCTGGCC 
AATGTCCTCG ACATCGACGA CGACTTCCGG CACACAATAT CTGGCTCCCA AACCGCCATT 
GGCTCGTCGG CCAGCTTCGG TCGGCCCACA ACGGTGGAGG AGCATCACAC GGCCATCGGC 
TGCAATCACA AAGATCTTCA TCTAATTCTC AAAGAATTGC AATTTATTAC GGCGCGGATG 
CGCAAAGCTG ACGACGAAGC GGAATTGATC GGCGATTGGA AGTTCGCGGC AATGGTTGTG 
GATAGATTTT GTTTAATTGT TTTCACGCTC TTCACGATTA TTGCAACGGT TACGGTGCTG 
CTCTCCGCTC CGCACATAAT CGTGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nAChRalpha6 ( NM_205951.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995673.1 Drosophila melanogaster nicotinic acetylcholine receptor alpha6, transcript variant B (nAChRalpha6), mRNA.

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CloneID	OFa14236
Clone ID Related Accession (Same CDS sequence)	NM_205951.2 , NM_001201835.3
Accession Version	NM_205951.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nicotinic acetylcholine receptor alpha6, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_205951.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACTCCC CGCTGCCAGC GTCGCTGTCG CTGTTTGTCC TGTTGATCTT TCTGGCGATA 
ATTAAAGAAA GCTGTCAAGG ACCTCATGAA AAGCGCCTGC TGAACCATCT GCTGTCCACC 
TACAATACGC TGGAGCGACC CGTGGCCAAT GAATCGGAGC CCCTGGAGGT TAAGTTCGGA 
CTGACGCTGC AGCAGATCAT CGACGTGGAC GAGAAGAATC AGCTTCTCAT AACGAATCTT 
TGGCTTTCGT TGGAGTGGAA CGACTACAAT CTGCGCTGGA ATGAAACGGA ATACGGCGGG 
GTCAAGGATC TACGAATCAC GCCCAACAAG CTGTGGAAGC CCGACGTGCT CATGTACAAC 
AGCGCGGATG AGGGATTCGA TGGCACGTAT CACACCAACA TTGTGGTCAA ACATAACGGC 
AGTTGTCTGT ACGTGCCCCC TGGTATCTTC AAGAGCACAT GCAAGATAGA CATCACGTGG 
TTCCCATTTG ATGACCAACA TTGCGAAATG AAATTCGGTA GTTGGACTTA CGATGGAAAT 
CAGTTGGATT TGGTTTTGAA TTCCGAAGAT GGAGGGGATC TTTCCGATTT CATAACAAAT 
GGCGAGTGGT ACTTGCTTGC CATGCCGGGA AAGAAGAATA CGATAGTCTA CGCCTGCTGC 
CCAGAACCAT ATGTCGATAT CACCTTTACT ATACAAATTC GTCGCCGTAC ATTATATTAT 
TTTTTCAATT TAATTGTGCC ATGTGTGCTA ATCTCATCGA TGGCCCTACT GGGCTTCACA 
TTGCCGCCGG ATTCGGGCGA GAAACTGACG CTGGGCGTAA CTATACTACT ATCATTAACA 
GTATTTCTAA ACCTTGTCGC CGAGTCCATG CCGACAACGT CGGATGCTGT TCCTCTTATA 
GGCACCTACT TCAATTGCAT CATGTTCATG GTCGCCTCGT CGGTGGTGCT GACAGTAGTG 
GTGCTCAACT ACCACCATCG CACAGCGGAC ATTCACGAGA TGCCACCGTG GATCAAGTCC 
GTTTTCCTAC AATGGCTGCC CTGGATCTTG CGAATGGGTC GACCCGGTCG CAAGATTACA 
CGCAAAACAA TACTATTAAG CAATCGCATG AAGGAGCTGG AGCTAAAGGA GCGCTCCTCC 
AAATCCCTGC TGGCCAATGT CCTCGACATC GACGACGACT TCCGGCACAC AATATCTGGC 
TCCCAAACCG CCATTGGCTC GTCGGCCAGC TTCGGTCGGC CCACAACGGT GGAGGAGCAT 
CACACGGCCA TCGGCTGCAA TCACAAAGAT CTTCATCTAA TTCTCAAAGA ATTGCAATTT 
ATTACGGCGC GGATGCGCAA AGCTGACGAC GAAGCGGAAT TGATCGGCGA TTGGAAGTTC 
GCGGCAATGG TTGTGGATAG ATTTTGTTTA ATTGTTTTCA CGCTCTTCAC GATTATTGCA 
ACGGTTACGG TGCTGCTCTC CGCTCCGCAC ATAATCGTGC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995673.1
CDS	1448..2932
Translation	MDSPLPASLSLFVLLIFLAIIKESCQGPHEKRLLNHLLSTYNTLERPVANESEPLEVKFGLTLQQIIDVDEKNQLLITNLWLSLEWNDYNLRWNETEYGGVKDLRITPNKLWKPDVLMYNSADEGFDGTYHTNIVVKHNGSCLYVPPGIFKSTCKIDITWFPFDDQHCEMKFGSWTYDGNQLDLVLNSEDGGDLSDFITNGEWYLLAMPGKKNTIVYACCPEPYVDITFTIQIRRRTLYYFFNLIVPCVLISSMALLGFTLPPDSGEKLTLGVTILLSLTVFLNLVAESMPTTSDAVPLIGTYFNCIMFMVASSVVLTVVVLNYHHRTADIHEMPPWIKSVFLQWLPWILRMGRPGRKITRKTILLSNRMKELELKERSSKSLLANVLDIDDDFRHTISGSQTAIGSSASFGRPTTVEEHHTAIGCNHKDLHLILKELQFITARMRKADDEAELIGDWKFAAMVVDRFCLIVFTLFTIIATVTVLLSAPHIIVQ

Target ORF information:

RefSeq Version	NM_205951.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nicotinic acetylcholine receptor alpha6, transcript variant B (nAChRalpha6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205951.2

ORF Insert Sequence:

ATGGACTCCC CGCTGCCAGC GTCGCTGTCG CTGTTTGTCC TGTTGATCTT TCTGGCGATA 
ATTAAAGAAA GCTGTCAAGG ACCTCATGAA AAGCGCCTGC TGAACCATCT GCTGTCCACC 
TACAATACGC TGGAGCGACC CGTGGCCAAT GAATCGGAGC CCCTGGAGGT TAAGTTCGGA 
CTGACGCTGC AGCAGATCAT CGACGTGGAC GAGAAGAATC AGCTTCTCAT AACGAATCTT 
TGGCTTTCGT TGGAGTGGAA CGACTACAAT CTGCGCTGGA ATGAAACGGA ATACGGCGGG 
GTCAAGGATC TACGAATCAC GCCCAACAAG CTGTGGAAGC CCGACGTGCT CATGTACAAC 
AGCGCGGATG AGGGATTCGA TGGCACGTAT CACACCAACA TTGTGGTCAA ACATAACGGC 
AGTTGTCTGT ACGTGCCCCC TGGTATCTTC AAGAGCACAT GCAAGATAGA CATCACGTGG 
TTCCCATTTG ATGACCAACA TTGCGAAATG AAATTCGGTA GTTGGACTTA CGATGGAAAT 
CAGTTGGATT TGGTTTTGAA TTCCGAAGAT GGAGGGGATC TTTCCGATTT CATAACAAAT 
GGCGAGTGGT ACTTGCTTGC CATGCCGGGA AAGAAGAATA CGATAGTCTA CGCCTGCTGC 
CCAGAACCAT ATGTCGATAT CACCTTTACT ATACAAATTC GTCGCCGTAC ATTATATTAT 
TTTTTCAATT TAATTGTGCC ATGTGTGCTA ATCTCATCGA TGGCCCTACT GGGCTTCACA 
TTGCCGCCGG ATTCGGGCGA GAAACTGACG CTGGGCGTAA CTATACTACT ATCATTAACA 
GTATTTCTAA ACCTTGTCGC CGAGTCCATG CCGACAACGT CGGATGCTGT TCCTCTTATA 
GGCACCTACT TCAATTGCAT CATGTTCATG GTCGCCTCGT CGGTGGTGCT GACAGTAGTG 
GTGCTCAACT ACCACCATCG CACAGCGGAC ATTCACGAGA TGCCACCGTG GATCAAGTCC 
GTTTTCCTAC AATGGCTGCC CTGGATCTTG CGAATGGGTC GACCCGGTCG CAAGATTACA 
CGCAAAACAA TACTATTAAG CAATCGCATG AAGGAGCTGG AGCTAAAGGA GCGCTCCTCC 
AAATCCCTGC TGGCCAATGT CCTCGACATC GACGACGACT TCCGGCACAC AATATCTGGC 
TCCCAAACCG CCATTGGCTC GTCGGCCAGC TTCGGTCGGC CCACAACGGT GGAGGAGCAT 
CACACGGCCA TCGGCTGCAA TCACAAAGAT CTTCATCTAA TTCTCAAAGA ATTGCAATTT 
ATTACGGCGC GGATGCGCAA AGCTGACGAC GAAGCGGAAT TGATCGGCGA TTGGAAGTTC 
GCGGCAATGG TTGTGGATAG ATTTTGTTTA ATTGTTTTCA CGCTCTTCAC GATTATTGCA 
ACGGTTACGG TGCTGCTCTC CGCTCCGCAC ATAATCGTGC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nAChRalpha6 ( NM_001201835.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188764.1 Drosophila melanogaster nicotinic acetylcholine receptor alpha6, transcript variant G (nAChRalpha6), mRNA.

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CloneID	OFa14236
Clone ID Related Accession (Same CDS sequence)	NM_205951.2 , NM_001201835.3
Accession Version	NM_001201835.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nicotinic acetylcholine receptor alpha6, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001201835.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACTCCC CGCTGCCAGC GTCGCTGTCG CTGTTTGTCC TGTTGATCTT TCTGGCGATA 
ATTAAAGAAA GCTGTCAAGG ACCTCATGAA AAGCGCCTGC TGAACCATCT GCTGTCCACC 
TACAATACGC TGGAGCGACC CGTGGCCAAT GAATCGGAGC CCCTGGAGGT TAAGTTCGGA 
CTGACGCTGC AGCAGATCAT CGACGTGGAC GAGAAGAATC AGCTTCTCAT AACGAATCTT 
TGGCTTTCGT TGGAGTGGAA CGACTACAAT CTGCGCTGGA ATGAAACGGA ATACGGCGGG 
GTCAAGGATC TACGAATCAC GCCCAACAAG CTGTGGAAGC CCGACGTGCT CATGTACAAC 
AGCGCGGATG AGGGATTCGA TGGCACGTAT CACACCAACA TTGTGGTCAA ACATAACGGC 
AGTTGTCTGT ACGTGCCCCC TGGTATCTTC AAGAGCACAT GCAAGATAGA CATCACGTGG 
TTCCCATTTG ATGACCAACA TTGCGAAATG AAATTCGGTA GTTGGACTTA CGATGGAAAT 
CAGTTGGATT TGGTTTTGAA TTCCGAAGAT GGAGGGGATC TTTCCGATTT CATAACAAAT 
GGCGAGTGGT ACTTGCTTGC CATGCCGGGA AAGAAGAATA CGATAGTCTA CGCCTGCTGC 
CCAGAACCAT ATGTCGATAT CACCTTTACT ATACAAATTC GTCGCCGTAC ATTATATTAT 
TTTTTCAATT TAATTGTGCC ATGTGTGCTA ATCTCATCGA TGGCCCTACT GGGCTTCACA 
TTGCCGCCGG ATTCGGGCGA GAAACTGACG CTGGGCGTAA CTATACTACT ATCATTAACA 
GTATTTCTAA ACCTTGTCGC CGAGTCCATG CCGACAACGT CGGATGCTGT TCCTCTTATA 
GGCACCTACT TCAATTGCAT CATGTTCATG GTCGCCTCGT CGGTGGTGCT GACAGTAGTG 
GTGCTCAACT ACCACCATCG CACAGCGGAC ATTCACGAGA TGCCACCGTG GATCAAGTCC 
GTTTTCCTAC AATGGCTGCC CTGGATCTTG CGAATGGGTC GACCCGGTCG CAAGATTACA 
CGCAAAACAA TACTATTAAG CAATCGCATG AAGGAGCTGG AGCTAAAGGA GCGCTCCTCC 
AAATCCCTGC TGGCCAATGT CCTCGACATC GACGACGACT TCCGGCACAC AATATCTGGC 
TCCCAAACCG CCATTGGCTC GTCGGCCAGC TTCGGTCGGC CCACAACGGT GGAGGAGCAT 
CACACGGCCA TCGGCTGCAA TCACAAAGAT CTTCATCTAA TTCTCAAAGA ATTGCAATTT 
ATTACGGCGC GGATGCGCAA AGCTGACGAC GAAGCGGAAT TGATCGGCGA TTGGAAGTTC 
GCGGCAATGG TTGTGGATAG ATTTTGTTTA ATTGTTTTCA CGCTCTTCAC GATTATTGCA 
ACGGTTACGG TGCTGCTCTC CGCTCCGCAC ATAATCGTGC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188764.1
CDS	2166..3650
Translation	MDSPLPASLSLFVLLIFLAIIKESCQGPHEKRLLNHLLSTYNTLERPVANESEPLEVKFGLTLQQIIDVDEKNQLLITNLWLSLEWNDYNLRWNETEYGGVKDLRITPNKLWKPDVLMYNSADEGFDGTYHTNIVVKHNGSCLYVPPGIFKSTCKIDITWFPFDDQHCEMKFGSWTYDGNQLDLVLNSEDGGDLSDFITNGEWYLLAMPGKKNTIVYACCPEPYVDITFTIQIRRRTLYYFFNLIVPCVLISSMALLGFTLPPDSGEKLTLGVTILLSLTVFLNLVAESMPTTSDAVPLIGTYFNCIMFMVASSVVLTVVVLNYHHRTADIHEMPPWIKSVFLQWLPWILRMGRPGRKITRKTILLSNRMKELELKERSSKSLLANVLDIDDDFRHTISGSQTAIGSSASFGRPTTVEEHHTAIGCNHKDLHLILKELQFITARMRKADDEAELIGDWKFAAMVVDRFCLIVFTLFTIIATVTVLLSAPHIIVQ

Target ORF information:

RefSeq Version	NM_001201835.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nicotinic acetylcholine receptor alpha6, transcript variant G (nAChRalpha6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201835.3

ORF Insert Sequence:

ATGGACTCCC CGCTGCCAGC GTCGCTGTCG CTGTTTGTCC TGTTGATCTT TCTGGCGATA 
ATTAAAGAAA GCTGTCAAGG ACCTCATGAA AAGCGCCTGC TGAACCATCT GCTGTCCACC 
TACAATACGC TGGAGCGACC CGTGGCCAAT GAATCGGAGC CCCTGGAGGT TAAGTTCGGA 
CTGACGCTGC AGCAGATCAT CGACGTGGAC GAGAAGAATC AGCTTCTCAT AACGAATCTT 
TGGCTTTCGT TGGAGTGGAA CGACTACAAT CTGCGCTGGA ATGAAACGGA ATACGGCGGG 
GTCAAGGATC TACGAATCAC GCCCAACAAG CTGTGGAAGC CCGACGTGCT CATGTACAAC 
AGCGCGGATG AGGGATTCGA TGGCACGTAT CACACCAACA TTGTGGTCAA ACATAACGGC 
AGTTGTCTGT ACGTGCCCCC TGGTATCTTC AAGAGCACAT GCAAGATAGA CATCACGTGG 
TTCCCATTTG ATGACCAACA TTGCGAAATG AAATTCGGTA GTTGGACTTA CGATGGAAAT 
CAGTTGGATT TGGTTTTGAA TTCCGAAGAT GGAGGGGATC TTTCCGATTT CATAACAAAT 
GGCGAGTGGT ACTTGCTTGC CATGCCGGGA AAGAAGAATA CGATAGTCTA CGCCTGCTGC 
CCAGAACCAT ATGTCGATAT CACCTTTACT ATACAAATTC GTCGCCGTAC ATTATATTAT 
TTTTTCAATT TAATTGTGCC ATGTGTGCTA ATCTCATCGA TGGCCCTACT GGGCTTCACA 
TTGCCGCCGG ATTCGGGCGA GAAACTGACG CTGGGCGTAA CTATACTACT ATCATTAACA 
GTATTTCTAA ACCTTGTCGC CGAGTCCATG CCGACAACGT CGGATGCTGT TCCTCTTATA 
GGCACCTACT TCAATTGCAT CATGTTCATG GTCGCCTCGT CGGTGGTGCT GACAGTAGTG 
GTGCTCAACT ACCACCATCG CACAGCGGAC ATTCACGAGA TGCCACCGTG GATCAAGTCC 
GTTTTCCTAC AATGGCTGCC CTGGATCTTG CGAATGGGTC GACCCGGTCG CAAGATTACA 
CGCAAAACAA TACTATTAAG CAATCGCATG AAGGAGCTGG AGCTAAAGGA GCGCTCCTCC 
AAATCCCTGC TGGCCAATGT CCTCGACATC GACGACGACT TCCGGCACAC AATATCTGGC 
TCCCAAACCG CCATTGGCTC GTCGGCCAGC TTCGGTCGGC CCACAACGGT GGAGGAGCAT 
CACACGGCCA TCGGCTGCAA TCACAAAGAT CTTCATCTAA TTCTCAAAGA ATTGCAATTT 
ATTACGGCGC GGATGCGCAA AGCTGACGAC GAAGCGGAAT TGATCGGCGA TTGGAAGTTC 
GCGGCAATGG TTGTGGATAG ATTTTGTTTA ATTGTTTTCA CGCTCTTCAC GATTATTGCA 
ACGGTTACGG TGCTGCTCTC CGCTCCGCAC ATAATCGTGC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nAChRalpha6 ( NM_164874.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723494.2 Drosophila melanogaster nicotinic acetylcholine receptor alpha6, transcript variant A (nAChRalpha6), mRNA.

Price & Availability↑

CloneID	OFa14237
Clone ID Related Accession (Same CDS sequence)	NM_164874.3
Accession Version	NM_164874.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nicotinic acetylcholine receptor alpha6, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164874.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACTCCC CGCTGCCAGC GTCGCTGTCG CTGTTTGTCC TGTTGATCTT TCTGGCGATA 
ATTAAAGAAA GCTGTCAAGG ACCTCATGAA AAGCGCCTGC TGAACCATCT GCTGTCCACC 
TACAATACGC TGGAGCGACC CGTGGCCAAT GAATCGGAGC CCCTGGAGGT TAAGTTCGGA 
CTGACGCTGC AGCAGATCAT CGACGTGGAT GAAAAGAATC AGATTCTGAC CACAAATGCG 
TGGTTAAATT TGGAGTGGAA CGACTACAAT CTGCGCTGGA ATGAAACGGA ATACGGCGGG 
GTCAAGGATC TACGAATCAC GCCCAACAAG CTGTGGAAGC CCGACGTGCT CATGTACAAC 
AGCGCGGATG AGGGATTCGA TGGCACGTAT CACACCAACA TTGTGGTCAA ACATAACGGC 
AGTTGTCTGT ACGTGCCCCC TGGTATCTTC AAGAGCACAT GCAAGATAGA CATCACGTGG 
TTCCCATTTG ATGACCAACA TTGCGAAATG AAATTCGGTA GTTGGACTTA CGATGGAAAT 
CAGTTGGATT TGGTTTTGAA TTCCGAAGAT GGAGGGGATC TTTCCGATTT CATAACAAAT 
GGCGAGTGGT ACTTGCTTGC CATGCCGGGA AAGAAGAATA CGATAGTCTA CGCCTGCTGC 
CCAGAACCAT ATGTCGATAT CACCTTTACT ATACAAATTC GTCGCCGTAC ATTATATTAT 
TTTTTCAATT TAATTGTGCC ATGTGTGCTA ATCTCATCGA TGGCCCTACT GGGCTTCACA 
TTGCCGCCGG ATTCGGGCGA GAAACTGACG CTGGGAGTTA CAATTCTTCT ATCGCTCACA 
GTGTTTCTCA ACCTTGTAGC TGAGACATTG CCCCAAGTAT CTGATGCAAT CCCCTTGTTA 
GGCACCTACT TCAATTGCAT CATGTTCATG GTCGCCTCGT CGGTGGTGCT GACAGTAGTG 
GTGCTCAACT ACCACCATCG CACAGCGGAC ATTCACGAGA TGCCACCGTG GATCAAGTCC 
GTTTTCCTAC AATGGCTGCC CTGGATCTTG CGAATGGGTC GACCCGGTCG CAAGATTACA 
CGCAAAACAA TACTATTAAG CAATCGCATG AAGGAGCTGG AGCTAAAGGA GCGCTCCTCC 
AAATCCCTGC TGGCCAATGT CCTCGACATC GACGACGACT TCCGGCACAC AATATCTGGC 
TCCCAAACCG CCATTGGCTC GTCGGCCAGC TTCGGTCGGC CCACAACGGT GGAGGAGCAT 
CACACGGCCA TCGGCTGCAA TCACAAAGAT CTTCATCTAA TTCTCAAAGA ATTGCAATTT 
ATTACGGCGC GGATGCGCAA AGCTGACGAC GAAGCGGAAT TGATCGGCGA TTGGAAGTTC 
GCGGCAATGG TTGTGGATAG ATTTTGTTTA ATTGTTTTCA CGCTCTTCAC GATTATTGCA 
ACGGTTACGG TGCTGCTCTC CGCTCCGCAC ATAATCGTGC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723494.2
CDS	2372..3856
Translation	MDSPLPASLSLFVLLIFLAIIKESCQGPHEKRLLNHLLSTYNTLERPVANESEPLEVKFGLTLQQIIDVDEKNQILTTNAWLNLEWNDYNLRWNETEYGGVKDLRITPNKLWKPDVLMYNSADEGFDGTYHTNIVVKHNGSCLYVPPGIFKSTCKIDITWFPFDDQHCEMKFGSWTYDGNQLDLVLNSEDGGDLSDFITNGEWYLLAMPGKKNTIVYACCPEPYVDITFTIQIRRRTLYYFFNLIVPCVLISSMALLGFTLPPDSGEKLTLGVTILLSLTVFLNLVAETLPQVSDAIPLLGTYFNCIMFMVASSVVLTVVVLNYHHRTADIHEMPPWIKSVFLQWLPWILRMGRPGRKITRKTILLSNRMKELELKERSSKSLLANVLDIDDDFRHTISGSQTAIGSSASFGRPTTVEEHHTAIGCNHKDLHLILKELQFITARMRKADDEAELIGDWKFAAMVVDRFCLIVFTLFTIIATVTVLLSAPHIIVQ

Target ORF information:

RefSeq Version	NM_164874.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nicotinic acetylcholine receptor alpha6, transcript variant A (nAChRalpha6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164874.3

ORF Insert Sequence:

ATGGACTCCC CGCTGCCAGC GTCGCTGTCG CTGTTTGTCC TGTTGATCTT TCTGGCGATA 
ATTAAAGAAA GCTGTCAAGG ACCTCATGAA AAGCGCCTGC TGAACCATCT GCTGTCCACC 
TACAATACGC TGGAGCGACC CGTGGCCAAT GAATCGGAGC CCCTGGAGGT TAAGTTCGGA 
CTGACGCTGC AGCAGATCAT CGACGTGGAT GAAAAGAATC AGATTCTGAC CACAAATGCG 
TGGTTAAATT TGGAGTGGAA CGACTACAAT CTGCGCTGGA ATGAAACGGA ATACGGCGGG 
GTCAAGGATC TACGAATCAC GCCCAACAAG CTGTGGAAGC CCGACGTGCT CATGTACAAC 
AGCGCGGATG AGGGATTCGA TGGCACGTAT CACACCAACA TTGTGGTCAA ACATAACGGC 
AGTTGTCTGT ACGTGCCCCC TGGTATCTTC AAGAGCACAT GCAAGATAGA CATCACGTGG 
TTCCCATTTG ATGACCAACA TTGCGAAATG AAATTCGGTA GTTGGACTTA CGATGGAAAT 
CAGTTGGATT TGGTTTTGAA TTCCGAAGAT GGAGGGGATC TTTCCGATTT CATAACAAAT 
GGCGAGTGGT ACTTGCTTGC CATGCCGGGA AAGAAGAATA CGATAGTCTA CGCCTGCTGC 
CCAGAACCAT ATGTCGATAT CACCTTTACT ATACAAATTC GTCGCCGTAC ATTATATTAT 
TTTTTCAATT TAATTGTGCC ATGTGTGCTA ATCTCATCGA TGGCCCTACT GGGCTTCACA 
TTGCCGCCGG ATTCGGGCGA GAAACTGACG CTGGGAGTTA CAATTCTTCT ATCGCTCACA 
GTGTTTCTCA ACCTTGTAGC TGAGACATTG CCCCAAGTAT CTGATGCAAT CCCCTTGTTA 
GGCACCTACT TCAATTGCAT CATGTTCATG GTCGCCTCGT CGGTGGTGCT GACAGTAGTG 
GTGCTCAACT ACCACCATCG CACAGCGGAC ATTCACGAGA TGCCACCGTG GATCAAGTCC 
GTTTTCCTAC AATGGCTGCC CTGGATCTTG CGAATGGGTC GACCCGGTCG CAAGATTACA 
CGCAAAACAA TACTATTAAG CAATCGCATG AAGGAGCTGG AGCTAAAGGA GCGCTCCTCC 
AAATCCCTGC TGGCCAATGT CCTCGACATC GACGACGACT TCCGGCACAC AATATCTGGC 
TCCCAAACCG CCATTGGCTC GTCGGCCAGC TTCGGTCGGC CCACAACGGT GGAGGAGCAT 
CACACGGCCA TCGGCTGCAA TCACAAAGAT CTTCATCTAA TTCTCAAAGA ATTGCAATTT 
ATTACGGCGC GGATGCGCAA AGCTGACGAC GAAGCGGAAT TGATCGGCGA TTGGAAGTTC 
GCGGCAATGG TTGTGGATAG ATTTTGTTTA ATTGTTTTCA CGCTCTTCAC GATTATTGCA 
ACGGTTACGG TGCTGCTCTC CGCTCCGCAC ATAATCGTGC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nAChRalpha6 ( NM_001273373.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260302.1 Drosophila melanogaster nicotinic acetylcholine receptor alpha6, transcript variant H (nAChRalpha6), mRNA.

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CloneID	OFa15963
Clone ID Related Accession (Same CDS sequence)	NM_001273373.2
Accession Version	NM_001273373.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nicotinic acetylcholine receptor alpha6, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001273373.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACTCCC CGCTGCCAGC GTCGCTGTCG CTGTTTGTCC TGTTGATCTT TCTGGCGATA 
ATTAAAGAAA GCTGTCAAGG ACCTCATGAA AAGCGCCTGC TGAACCATCT GCTGTCCACC 
TACAATACGC TGGAGCGACC CGTGGCCAAT GAATCGGAGC CCCTGGAGGT TAAGTTCGGA 
CTGACGCTGC AGCAGATCAT CGACGTGGAT GAAAAGAATC AGATTCTGAC CACAAATGCG 
TGGTTAAATT TGGAGTGGAA CGACTACAAT CTGCGCTGGA ATGAAACGGA ATACGGCGGG 
GTCAAGGATC TACGAATCAC GCCCAACAAG CTGTGGAAGC CCGACGTGCT CATGTACAAC 
AGCGCGGATG AGGGATTCGA TGGCACGTAT CACACCAACA TTGTGGTCAA ACATAACGGC 
AGTTGTCTGT ACGTGCCCCC TGGTATCTTC AAGAGCACAT GCAAGATAGA CATCACGTGG 
TTCCCATTTG ATGACCAACA TTGCGAAATG AAATTCGGTA GTTGGACTTA CGATGGAAAT 
CAGTTGGATT TGGTTTTGAA TTCCGAAGAT GGAGGGGATC TTTCCGATTT CATAACAAAT 
GGCGAGTGGT ACTTGCTTGC CATGCCGGGA AAGAAGAATA CGATAGTCTA CGCCTGCTGC 
CCAGAACCAT ATGTCGATAT CACCTTTACT ATACAAATTC GTCGCCGTAC ATTATATTAT 
TTTTTCAATT TAATTGTGCC ATGTGTGCTA ATCTCATCGA TGGCCCTACT GGGCTTCACA 
TTGCCGCCGG ATTCGGGCGA GAAACTGACG CTGGGCGTAA CTATACTACT ATCATTAACA 
GTATTTCTAA ACCTTGTCGC CGAGTCCATG CCGACAACGT CGGATGCTGT TCCTCTTATA 
GGCACCTACT TCAATTGCAT CATGTTCATG GTCGCCTCGT CGGTGGTGCT GACAGTAGTG 
GTGCTCAACT ACCACCATCG CACAGCGGAC ATTCACGAGA TGCCACCGTG GATCAAGTCC 
GTTTTCCTAC AATGGCTGCC CTGGATCTTG CGAATGGGTC GACCCGGTCG CAAGATTACA 
CGCAAAACAA TACTATTAAG CAATCGCATG AAGGAGCTGG AGCTAAAGGA GCGCTCCTCC 
AAATCCCTGC TGGCCAATGT CCTCGACATC GACGACGACT TCCGGCACAC AATATCTGGC 
TCCCAAACCG CCATTGGCTC GTCGGCCAGC TTCGGTCGGC CCACAACGGT GGAGGAGCAT 
CACACGGCCA TCGGCTGCAA TCACAAAGAT CTTCATCTAA TTCTCAAAGA ATTGCAATTT 
ATTACGGCGC GGATGCGCAA AGCTGACGAC GAAGCGGAAT TGATCGGCGA TTGGAAGTTC 
GCGGCAATGG TTGTGGATAG ATTTTGTTTA ATTGTTTTCA CGCTCTTCAC GATTATTGCA 
ACGGTTACGG TGCTGCTCTC CGCTCCGCAC ATAATCGTGC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260302.1
CDS	2166..3650
Translation	MDSPLPASLSLFVLLIFLAIIKESCQGPHEKRLLNHLLSTYNTLERPVANESEPLEVKFGLTLQQIIDVDEKNQILTTNAWLNLEWNDYNLRWNETEYGGVKDLRITPNKLWKPDVLMYNSADEGFDGTYHTNIVVKHNGSCLYVPPGIFKSTCKIDITWFPFDDQHCEMKFGSWTYDGNQLDLVLNSEDGGDLSDFITNGEWYLLAMPGKKNTIVYACCPEPYVDITFTIQIRRRTLYYFFNLIVPCVLISSMALLGFTLPPDSGEKLTLGVTILLSLTVFLNLVAESMPTTSDAVPLIGTYFNCIMFMVASSVVLTVVVLNYHHRTADIHEMPPWIKSVFLQWLPWILRMGRPGRKITRKTILLSNRMKELELKERSSKSLLANVLDIDDDFRHTISGSQTAIGSSASFGRPTTVEEHHTAIGCNHKDLHLILKELQFITARMRKADDEAELIGDWKFAAMVVDRFCLIVFTLFTIIATVTVLLSAPHIIVQ

Target ORF information:

RefSeq Version	NM_001273373.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nicotinic acetylcholine receptor alpha6, transcript variant H (nAChRalpha6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273373.2

ORF Insert Sequence:

ATGGACTCCC CGCTGCCAGC GTCGCTGTCG CTGTTTGTCC TGTTGATCTT TCTGGCGATA 
ATTAAAGAAA GCTGTCAAGG ACCTCATGAA AAGCGCCTGC TGAACCATCT GCTGTCCACC 
TACAATACGC TGGAGCGACC CGTGGCCAAT GAATCGGAGC CCCTGGAGGT TAAGTTCGGA 
CTGACGCTGC AGCAGATCAT CGACGTGGAT GAAAAGAATC AGATTCTGAC CACAAATGCG 
TGGTTAAATT TGGAGTGGAA CGACTACAAT CTGCGCTGGA ATGAAACGGA ATACGGCGGG 
GTCAAGGATC TACGAATCAC GCCCAACAAG CTGTGGAAGC CCGACGTGCT CATGTACAAC 
AGCGCGGATG AGGGATTCGA TGGCACGTAT CACACCAACA TTGTGGTCAA ACATAACGGC 
AGTTGTCTGT ACGTGCCCCC TGGTATCTTC AAGAGCACAT GCAAGATAGA CATCACGTGG 
TTCCCATTTG ATGACCAACA TTGCGAAATG AAATTCGGTA GTTGGACTTA CGATGGAAAT 
CAGTTGGATT TGGTTTTGAA TTCCGAAGAT GGAGGGGATC TTTCCGATTT CATAACAAAT 
GGCGAGTGGT ACTTGCTTGC CATGCCGGGA AAGAAGAATA CGATAGTCTA CGCCTGCTGC 
CCAGAACCAT ATGTCGATAT CACCTTTACT ATACAAATTC GTCGCCGTAC ATTATATTAT 
TTTTTCAATT TAATTGTGCC ATGTGTGCTA ATCTCATCGA TGGCCCTACT GGGCTTCACA 
TTGCCGCCGG ATTCGGGCGA GAAACTGACG CTGGGCGTAA CTATACTACT ATCATTAACA 
GTATTTCTAA ACCTTGTCGC CGAGTCCATG CCGACAACGT CGGATGCTGT TCCTCTTATA 
GGCACCTACT TCAATTGCAT CATGTTCATG GTCGCCTCGT CGGTGGTGCT GACAGTAGTG 
GTGCTCAACT ACCACCATCG CACAGCGGAC ATTCACGAGA TGCCACCGTG GATCAAGTCC 
GTTTTCCTAC AATGGCTGCC CTGGATCTTG CGAATGGGTC GACCCGGTCG CAAGATTACA 
CGCAAAACAA TACTATTAAG CAATCGCATG AAGGAGCTGG AGCTAAAGGA GCGCTCCTCC 
AAATCCCTGC TGGCCAATGT CCTCGACATC GACGACGACT TCCGGCACAC AATATCTGGC 
TCCCAAACCG CCATTGGCTC GTCGGCCAGC TTCGGTCGGC CCACAACGGT GGAGGAGCAT 
CACACGGCCA TCGGCTGCAA TCACAAAGAT CTTCATCTAA TTCTCAAAGA ATTGCAATTT 
ATTACGGCGC GGATGCGCAA AGCTGACGAC GAAGCGGAAT TGATCGGCGA TTGGAAGTTC 
GCGGCAATGG TTGTGGATAG ATTTTGTTTA ATTGTTTTCA CGCTCTTCAC GATTATTGCA 
ACGGTTACGG TGCTGCTCTC CGCTCCGCAC ATAATCGTGC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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nAChRalpha6 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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