Mco1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mco1
Entrez Gene ID	34258
Full Name	Multicopper oxidase-1
Synonyms	CG3759,Dmel\CG3759,MCO1
General protein information	Preferred Names Multicopper oxidase-1 Names CG3759 gene product from transcript CG3759-RB CG3759-PA CG3759-PB CG3759-PC Mco1-PA Mco1-PB Mco1-PC laccase-like multicopper oxidase-1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 30B2-30B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_135443.5	NP_609287.3	multicopper oxidase 1, isoform A
NM_001201825.2	NP_001188754.1	multicopper oxidase 1, isoform B
NM_001201826.2	NP_001188755.1	multicopper oxidase 1, isoform C

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_08127	XP_003715087.1
Drosophila melanogaster (fruit fly)	Mco1	NP_001188754.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003738	XP_310270.2
Neurospora crassa	NCU04528	XP_956939.1
Neurospora crassa	NCU05113	XP_957099.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

MCO1 oxidizes ferrous iron in the hemolymph and that the resulting ferric iron is bound by transferrin or melanotransferrin, leading to iron storage, iron withholding from pathogens, regulation of oxidative stress, and/or epithelial maturation.
Title: Multicopper oxidase-1 is a ferroxidase essential for iron homeostasis in Drosophila melanogaster.

The following Mco1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mco1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa14191	NM_001201825.2 Latest version!	Drosophila melanogaster multicopper oxidase 1, transcript variant B (Mco1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa14191	NM_135443.5 Latest version!	Drosophila melanogaster multicopper oxidase 1, transcript variant A (Mco1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa14191	NM_001201826.2 Latest version!	Drosophila melanogaster multicopper oxidase 1, transcript variant C (Mco1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mco1 ( NM_135443.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609287.3 Drosophila melanogaster multicopper oxidase 1, transcript variant A (Mco1), mRNA.

Price & Availability↑

CloneID	OFa14191
Clone ID Related Accession (Same CDS sequence)	NM_135443.5 , NM_001201825.2 , NM_001201826.2
Accession Version	NM_135443.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2880bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	multicopper oxidase 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_135443.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGTAA GCCATAATTA TGACCAAATG AGCCAGCCAA CAAACACAAC AACAACGCAA 
ACAAAAGCAT CGAAAAGGCC AAGCAGATTC AACAGCAAAA TGCCGGTTGT CAACAGCAGA 
TTAGCAGAGT TAGCTCGGAT AACAGTACTC TTAACAGCAC TGCTTGTGAG CTACGCCAGT 
GGCTTGCAAA CTGTAGGCGA ATCAGCAGAT GATAATACCA TCTGCCTGTA TCGAGAGTAC 
AACACCACTT ATCGAAAGGA GATTGGCGCA GTGTGGTTTG CCTCAAAGGA TCCATGTTTG 
GTGTATTCCT GTGCAGCTGG TGTGGCCAAG GATCCACAGG CGCATATTGT GGCCACCCAA 
GTCGACTGCA ATGAGTTCTA CTGTGAAGTG GGTTCCGAGC TGCGTAGTGT CGAAGGCAGT 
TGCTGCGGGG AGTGTGTAAG GACTCACTGT CAACACAACC ACACTCTGTA TGCGGTGGGT 
GAATCATGGC ACAATGATGC CGATTGCACG CTCATCGAAT GTGGACGTCT GGATAATGGA 
CAGATTGTAA TGAATACATA CAAGAGAAAT TGTCCGGCTT TGACTGAAGA TTGTCCAGCT 
TCAAGATTGG AGGAGCGAAA CTGTTGTCCC TACTGCAGGC CCCTCCAAAC GGCCAGGATT 
GAGGAGCTGC AGGACAATGT AGCGGAGAGC ACGGATGATA TTTGGACAGC CGAATGGTAT 
CGAAACCATC CCTGCAATCG GGATTGTCAA GTGGGAGCCG AACCCATGAC CTGTCGCTAC 
AAGTTCGTGG TGGAATGGTA CCAGACCTTT TCCAAAGCCT GCTACGATTG TCCACGGAAT 
CTTACGGACT GCTCCCGACC ACATTGTGTC ATGGGCGATG GTTTAGAGCG GAGTATCACT 
GTGGTCAATC GTATGATGCC CGGGCCAGCG ATTGAAGTCT GTGAGGGCGA TGAAATAGTG 
GTCGATGTGA AGAATCATTT GTTGGGCGAA AGCACATCGA TTCACTGGCA TGGTCTGCAC 
CAGAAGAAGA CACCCTATAT GGATGGTGTA CCACACATCA CTCAGTGTCC AATTACTCCA 
CATGCGACCT TCAGGTATTC CTTTCCAGCC GATCTCTCCG GCACTCACTT CTGGCATTCG 
CACACTGGCA TGCAGCGAGG TGATGGAGTT TTCGGTGCCT TAATCATCCG GAAACCAAAG 
ACAGCCGAAC CACACGGAGG ACTCTACGAC TTTGACCTCA GCGAGCATGT GATGATTGTC 
CAGGATTGGA TACATGACAC AGGTGCCAGC ATATTTTCCT ATCATCATCA CTCCCGAGGT 
GATAATAAGC CCCACAATCT GCTCGTGAAC GGCAAGGGAC GTTACTATAA TCGCATTTGG 
GCGGAAGCGA AGCAAGCTCA TCGCAGAGCT GAGGAGAGAA CTACCCAACC GGTGGAACCA 
TTGCCCAAGT CCCAGGTGGA CTTCGTTCAG ACACTACCGA GACAAGCTCG ATTGGCTAAA 
ACTAATACAA CCAAACTATT TCCCGTGAAT TCCCGCCAAA AGAGGGGTAA TTTAAATGAG 
ATACCCTTGG AACTAGTTCC CCATCAGATT TACACAGTGA GAAGAGGTTT TCGTTATCGC 
TTCAGAATTA TAAATGCGGA ATATCTAAAC TGTCCTATTG TGGTGTCTAT TGACGGTCAT 
AACTTGACAG CCATAAACTC GGATGGCTTT GATATTGAGG CAATGGACGT GGGCTCTATA 
GTTACCTATT CCGGTGAGCG ATTTGATTTT GTACTAAATG CCAATCTCGA GGTTGGAAAC 
TATTGGATTC GACTAAAGGG TTTGATGGAC TGCAGCGAAG TCTTTACTTC CGCCTTTCAA 
GTGGCCATTC TTCGCTATGA GGGAGCTCCA GATGAAGAGC CTACTGCGGA ATTGAGTTAC 
GGCCACAAGG CTGAGGGCAT TGAACTGAAT GTGATGAACC GCGGTCCTGG TTACCCGGAC 
ACCAAAACCG TTGCAGAAAT GAGGGCTCTG CCCATCTATG ATCATGTCTC GGGCATCGAT 
CACGATACAC TGAAACCGGA AGCGGACTAC AAGTTCTTCA TCTACTACGA TTTCTATACT 
AAGAATAATC CCGATTTTCA CGATAAAGAT CTTTATGCCA TGGACATGGA GATGACCCAG 
CAGAATCGCC TATACACGCC TCAACTGAAT CATATAACCT TAAATTTTCC TTCGCTAGCA 
CTGCTACCTT CCAGGAGTCA GCTAAAGGAC TCGGACTTCT GTAACGAAAC GAGCTTAATG 
GATCAGGGTA TCGATTGTCG CCAGGAGTTC TGCAAGTGTC ATCATGTCCT GCAGGTTCCA 
TTGGGCGCCG TGGTCGAGAT GATCATCGTG GACGAGGGAT TCCAATACTA TGCCAATCAT 
CCCTTTCATT TGCATGGAAA CGCCTTCAGA GTCATGGGAT TGGAGCGTTT GGGCGAAAAT 
GTCACCGTTG AAATGATAAA ACAACTGGAT CAGTTCAATC TACTCAAGCG GAATCTGGAC 
AATCCACCCG TGAAGGACAC AGTTACTATT CCAGATGGAG GCTATACCAT CATCAGATTC 
GAGGCCTCTA ATCCGGGCTA TTGGCTTTTC CATTGCCACA TCGAATTCCA TGCCGAGATC 
GGTATGGCTC TGGTCTTCAA AGTGGGTAAC GACGATCAAA TGGTTCCGGT GCCAGAGAAC 
TTTCCCACCT GCGGCGATTA TAATCCAGAC TTAAGATCTG ATGGAGGAAC TACAGAAGAT 
TCGGGATCCA GCAAGCCGAT AACAGCTACT CCCCCAAATA CAGGAGGCGG TGGATCGGGT 
CTGGAACCCA CTTGGATTAC ATTGATGATT AGTTCCATGC TAGTGAAACT CTATCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609287.3
CDS	184..3063
Translation	MSVSHNYDQMSQPTNTTTTQTKASKRPSRFNSKMPVVNSRLAELARITVLLTALLVSYASGLQTVGESADDNTICLYREYNTTYRKEIGAVWFASKDPCLVYSCAAGVAKDPQAHIVATQVDCNEFYCEVGSELRSVEGSCCGECVRTHCQHNHTLYAVGESWHNDADCTLIECGRLDNGQIVMNTYKRNCPALTEDCPASRLEERNCCPYCRPLQTARIEELQDNVAESTDDIWTAEWYRNHPCNRDCQVGAEPMTCRYKFVVEWYQTFSKACYDCPRNLTDCSRPHCVMGDGLERSITVVNRMMPGPAIEVCEGDEIVVDVKNHLLGESTSIHWHGLHQKKTPYMDGVPHITQCPITPHATFRYSFPADLSGTHFWHSHTGMQRGDGVFGALIIRKPKTAEPHGGLYDFDLSEHVMIVQDWIHDTGASIFSYHHHSRGDNKPHNLLVNGKGRYYNRIWAEAKQAHRRAEERTTQPVEPLPKSQVDFVQTLPRQARLAKTNTTKLFPVNSRQKRGNLNEIPLELVPHQIYTVRRGFRYRFRIINAEYLNCPIVVSIDGHNLTAINSDGFDIEAMDVGSIVTYSGERFDFVLNANLEVGNYWIRLKGLMDCSEVFTSAFQVAILRYEGAPDEEPTAELSYGHKAEGIELNVMNRGPGYPDTKTVAEMRALPIYDHVSGIDHDTLKPEADYKFFIYYDFYTKNNPDFHDKDLYAMDMEMTQQNRLYTPQLNHITLNFPSLALLPSRSQLKDSDFCNETSLMDQGIDCRQEFCKCHHVLQVPLGAVVEMIIVDEGFQYYANHPFHLHGNAFRVMGLERLGENVTVEMIKQLDQFNLLKRNLDNPPVKDTVTIPDGGYTIIRFEASNPGYWLFHCHIEFHAEIGMALVFKVGNDDQMVPVPENFPTCGDYNPDLRSDGGTTEDSGSSKPITATPPNTGGGGSGLEPTWITLMISSMLVKLYR

Target ORF information:

RefSeq Version	NM_135443.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster multicopper oxidase 1, transcript variant A (Mco1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135443.5

ORF Insert Sequence:

ATGAGCGTAA GCCATAATTA TGACCAAATG AGCCAGCCAA CAAACACAAC AACAACGCAA 
ACAAAAGCAT CGAAAAGGCC AAGCAGATTC AACAGCAAAA TGCCGGTTGT CAACAGCAGA 
TTAGCAGAGT TAGCTCGGAT AACAGTACTC TTAACAGCAC TGCTTGTGAG CTACGCCAGT 
GGCTTGCAAA CTGTAGGCGA ATCAGCAGAT GATAATACCA TCTGCCTGTA TCGAGAGTAC 
AACACCACTT ATCGAAAGGA GATTGGCGCA GTGTGGTTTG CCTCAAAGGA TCCATGTTTG 
GTGTATTCCT GTGCAGCTGG TGTGGCCAAG GATCCACAGG CGCATATTGT GGCCACCCAA 
GTCGACTGCA ATGAGTTCTA CTGTGAAGTG GGTTCCGAGC TGCGTAGTGT CGAAGGCAGT 
TGCTGCGGGG AGTGTGTAAG GACTCACTGT CAACACAACC ACACTCTGTA TGCGGTGGGT 
GAATCATGGC ACAATGATGC CGATTGCACG CTCATCGAAT GTGGACGTCT GGATAATGGA 
CAGATTGTAA TGAATACATA CAAGAGAAAT TGTCCGGCTT TGACTGAAGA TTGTCCAGCT 
TCAAGATTGG AGGAGCGAAA CTGTTGTCCC TACTGCAGGC CCCTCCAAAC GGCCAGGATT 
GAGGAGCTGC AGGACAATGT AGCGGAGAGC ACGGATGATA TTTGGACAGC CGAATGGTAT 
CGAAACCATC CCTGCAATCG GGATTGTCAA GTGGGAGCCG AACCCATGAC CTGTCGCTAC 
AAGTTCGTGG TGGAATGGTA CCAGACCTTT TCCAAAGCCT GCTACGATTG TCCACGGAAT 
CTTACGGACT GCTCCCGACC ACATTGTGTC ATGGGCGATG GTTTAGAGCG GAGTATCACT 
GTGGTCAATC GTATGATGCC CGGGCCAGCG ATTGAAGTCT GTGAGGGCGA TGAAATAGTG 
GTCGATGTGA AGAATCATTT GTTGGGCGAA AGCACATCGA TTCACTGGCA TGGTCTGCAC 
CAGAAGAAGA CACCCTATAT GGATGGTGTA CCACACATCA CTCAGTGTCC AATTACTCCA 
CATGCGACCT TCAGGTATTC CTTTCCAGCC GATCTCTCCG GCACTCACTT CTGGCATTCG 
CACACTGGCA TGCAGCGAGG TGATGGAGTT TTCGGTGCCT TAATCATCCG GAAACCAAAG 
ACAGCCGAAC CACACGGAGG ACTCTACGAC TTTGACCTCA GCGAGCATGT GATGATTGTC 
CAGGATTGGA TACATGACAC AGGTGCCAGC ATATTTTCCT ATCATCATCA CTCCCGAGGT 
GATAATAAGC CCCACAATCT GCTCGTGAAC GGCAAGGGAC GTTACTATAA TCGCATTTGG 
GCGGAAGCGA AGCAAGCTCA TCGCAGAGCT GAGGAGAGAA CTACCCAACC GGTGGAACCA 
TTGCCCAAGT CCCAGGTGGA CTTCGTTCAG ACACTACCGA GACAAGCTCG ATTGGCTAAA 
ACTAATACAA CCAAACTATT TCCCGTGAAT TCCCGCCAAA AGAGGGGTAA TTTAAATGAG 
ATACCCTTGG AACTAGTTCC CCATCAGATT TACACAGTGA GAAGAGGTTT TCGTTATCGC 
TTCAGAATTA TAAATGCGGA ATATCTAAAC TGTCCTATTG TGGTGTCTAT TGACGGTCAT 
AACTTGACAG CCATAAACTC GGATGGCTTT GATATTGAGG CAATGGACGT GGGCTCTATA 
GTTACCTATT CCGGTGAGCG ATTTGATTTT GTACTAAATG CCAATCTCGA GGTTGGAAAC 
TATTGGATTC GACTAAAGGG TTTGATGGAC TGCAGCGAAG TCTTTACTTC CGCCTTTCAA 
GTGGCCATTC TTCGCTATGA GGGAGCTCCA GATGAAGAGC CTACTGCGGA ATTGAGTTAC 
GGCCACAAGG CTGAGGGCAT TGAACTGAAT GTGATGAACC GCGGTCCTGG TTACCCGGAC 
ACCAAAACCG TTGCAGAAAT GAGGGCTCTG CCCATCTATG ATCATGTCTC GGGCATCGAT 
CACGATACAC TGAAACCGGA AGCGGACTAC AAGTTCTTCA TCTACTACGA TTTCTATACT 
AAGAATAATC CCGATTTTCA CGATAAAGAT CTTTATGCCA TGGACATGGA GATGACCCAG 
CAGAATCGCC TATACACGCC TCAACTGAAT CATATAACCT TAAATTTTCC TTCGCTAGCA 
CTGCTACCTT CCAGGAGTCA GCTAAAGGAC TCGGACTTCT GTAACGAAAC GAGCTTAATG 
GATCAGGGTA TCGATTGTCG CCAGGAGTTC TGCAAGTGTC ATCATGTCCT GCAGGTTCCA 
TTGGGCGCCG TGGTCGAGAT GATCATCGTG GACGAGGGAT TCCAATACTA TGCCAATCAT 
CCCTTTCATT TGCATGGAAA CGCCTTCAGA GTCATGGGAT TGGAGCGTTT GGGCGAAAAT 
GTCACCGTTG AAATGATAAA ACAACTGGAT CAGTTCAATC TACTCAAGCG GAATCTGGAC 
AATCCACCCG TGAAGGACAC AGTTACTATT CCAGATGGAG GCTATACCAT CATCAGATTC 
GAGGCCTCTA ATCCGGGCTA TTGGCTTTTC CATTGCCACA TCGAATTCCA TGCCGAGATC 
GGTATGGCTC TGGTCTTCAA AGTGGGTAAC GACGATCAAA TGGTTCCGGT GCCAGAGAAC 
TTTCCCACCT GCGGCGATTA TAATCCAGAC TTAAGATCTG ATGGAGGAAC TACAGAAGAT 
TCGGGATCCA GCAAGCCGAT AACAGCTACT CCCCCAAATA CAGGAGGCGG TGGATCGGGT 
CTGGAACCCA CTTGGATTAC ATTGATGATT AGTTCCATGC TAGTGAAACT CTATCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mco1 ( NM_001201825.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188754.1 Drosophila melanogaster multicopper oxidase 1, transcript variant B (Mco1), mRNA.

Price & Availability↑

CloneID	OFa14191
Clone ID Related Accession (Same CDS sequence)	NM_135443.5 , NM_001201825.2 , NM_001201826.2
Accession Version	NM_001201825.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2880bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	multicopper oxidase 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001201825.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGTAA GCCATAATTA TGACCAAATG AGCCAGCCAA CAAACACAAC AACAACGCAA 
ACAAAAGCAT CGAAAAGGCC AAGCAGATTC AACAGCAAAA TGCCGGTTGT CAACAGCAGA 
TTAGCAGAGT TAGCTCGGAT AACAGTACTC TTAACAGCAC TGCTTGTGAG CTACGCCAGT 
GGCTTGCAAA CTGTAGGCGA ATCAGCAGAT GATAATACCA TCTGCCTGTA TCGAGAGTAC 
AACACCACTT ATCGAAAGGA GATTGGCGCA GTGTGGTTTG CCTCAAAGGA TCCATGTTTG 
GTGTATTCCT GTGCAGCTGG TGTGGCCAAG GATCCACAGG CGCATATTGT GGCCACCCAA 
GTCGACTGCA ATGAGTTCTA CTGTGAAGTG GGTTCCGAGC TGCGTAGTGT CGAAGGCAGT 
TGCTGCGGGG AGTGTGTAAG GACTCACTGT CAACACAACC ACACTCTGTA TGCGGTGGGT 
GAATCATGGC ACAATGATGC CGATTGCACG CTCATCGAAT GTGGACGTCT GGATAATGGA 
CAGATTGTAA TGAATACATA CAAGAGAAAT TGTCCGGCTT TGACTGAAGA TTGTCCAGCT 
TCAAGATTGG AGGAGCGAAA CTGTTGTCCC TACTGCAGGC CCCTCCAAAC GGCCAGGATT 
GAGGAGCTGC AGGACAATGT AGCGGAGAGC ACGGATGATA TTTGGACAGC CGAATGGTAT 
CGAAACCATC CCTGCAATCG GGATTGTCAA GTGGGAGCCG AACCCATGAC CTGTCGCTAC 
AAGTTCGTGG TGGAATGGTA CCAGACCTTT TCCAAAGCCT GCTACGATTG TCCACGGAAT 
CTTACGGACT GCTCCCGACC ACATTGTGTC ATGGGCGATG GTTTAGAGCG GAGTATCACT 
GTGGTCAATC GTATGATGCC CGGGCCAGCG ATTGAAGTCT GTGAGGGCGA TGAAATAGTG 
GTCGATGTGA AGAATCATTT GTTGGGCGAA AGCACATCGA TTCACTGGCA TGGTCTGCAC 
CAGAAGAAGA CACCCTATAT GGATGGTGTA CCACACATCA CTCAGTGTCC AATTACTCCA 
CATGCGACCT TCAGGTATTC CTTTCCAGCC GATCTCTCCG GCACTCACTT CTGGCATTCG 
CACACTGGCA TGCAGCGAGG TGATGGAGTT TTCGGTGCCT TAATCATCCG GAAACCAAAG 
ACAGCCGAAC CACACGGAGG ACTCTACGAC TTTGACCTCA GCGAGCATGT GATGATTGTC 
CAGGATTGGA TACATGACAC AGGTGCCAGC ATATTTTCCT ATCATCATCA CTCCCGAGGT 
GATAATAAGC CCCACAATCT GCTCGTGAAC GGCAAGGGAC GTTACTATAA TCGCATTTGG 
GCGGAAGCGA AGCAAGCTCA TCGCAGAGCT GAGGAGAGAA CTACCCAACC GGTGGAACCA 
TTGCCCAAGT CCCAGGTGGA CTTCGTTCAG ACACTACCGA GACAAGCTCG ATTGGCTAAA 
ACTAATACAA CCAAACTATT TCCCGTGAAT TCCCGCCAAA AGAGGGGTAA TTTAAATGAG 
ATACCCTTGG AACTAGTTCC CCATCAGATT TACACAGTGA GAAGAGGTTT TCGTTATCGC 
TTCAGAATTA TAAATGCGGA ATATCTAAAC TGTCCTATTG TGGTGTCTAT TGACGGTCAT 
AACTTGACAG CCATAAACTC GGATGGCTTT GATATTGAGG CAATGGACGT GGGCTCTATA 
GTTACCTATT CCGGTGAGCG ATTTGATTTT GTACTAAATG CCAATCTCGA GGTTGGAAAC 
TATTGGATTC GACTAAAGGG TTTGATGGAC TGCAGCGAAG TCTTTACTTC CGCCTTTCAA 
GTGGCCATTC TTCGCTATGA GGGAGCTCCA GATGAAGAGC CTACTGCGGA ATTGAGTTAC 
GGCCACAAGG CTGAGGGCAT TGAACTGAAT GTGATGAACC GCGGTCCTGG TTACCCGGAC 
ACCAAAACCG TTGCAGAAAT GAGGGCTCTG CCCATCTATG ATCATGTCTC GGGCATCGAT 
CACGATACAC TGAAACCGGA AGCGGACTAC AAGTTCTTCA TCTACTACGA TTTCTATACT 
AAGAATAATC CCGATTTTCA CGATAAAGAT CTTTATGCCA TGGACATGGA GATGACCCAG 
CAGAATCGCC TATACACGCC TCAACTGAAT CATATAACCT TAAATTTTCC TTCGCTAGCA 
CTGCTACCTT CCAGGAGTCA GCTAAAGGAC TCGGACTTCT GTAACGAAAC GAGCTTAATG 
GATCAGGGTA TCGATTGTCG CCAGGAGTTC TGCAAGTGTC ATCATGTCCT GCAGGTTCCA 
TTGGGCGCCG TGGTCGAGAT GATCATCGTG GACGAGGGAT TCCAATACTA TGCCAATCAT 
CCCTTTCATT TGCATGGAAA CGCCTTCAGA GTCATGGGAT TGGAGCGTTT GGGCGAAAAT 
GTCACCGTTG AAATGATAAA ACAACTGGAT CAGTTCAATC TACTCAAGCG GAATCTGGAC 
AATCCACCCG TGAAGGACAC AGTTACTATT CCAGATGGAG GCTATACCAT CATCAGATTC 
GAGGCCTCTA ATCCGGGCTA TTGGCTTTTC CATTGCCACA TCGAATTCCA TGCCGAGATC 
GGTATGGCTC TGGTCTTCAA AGTGGGTAAC GACGATCAAA TGGTTCCGGT GCCAGAGAAC 
TTTCCCACCT GCGGCGATTA TAATCCAGAC TTAAGATCTG ATGGAGGAAC TACAGAAGAT 
TCGGGATCCA GCAAGCCGAT AACAGCTACT CCCCCAAATA CAGGAGGCGG TGGATCGGGT 
CTGGAACCCA CTTGGATTAC ATTGATGATT AGTTCCATGC TAGTGAAACT CTATCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188754.1
CDS	216..3095
Translation	MSVSHNYDQMSQPTNTTTTQTKASKRPSRFNSKMPVVNSRLAELARITVLLTALLVSYASGLQTVGESADDNTICLYREYNTTYRKEIGAVWFASKDPCLVYSCAAGVAKDPQAHIVATQVDCNEFYCEVGSELRSVEGSCCGECVRTHCQHNHTLYAVGESWHNDADCTLIECGRLDNGQIVMNTYKRNCPALTEDCPASRLEERNCCPYCRPLQTARIEELQDNVAESTDDIWTAEWYRNHPCNRDCQVGAEPMTCRYKFVVEWYQTFSKACYDCPRNLTDCSRPHCVMGDGLERSITVVNRMMPGPAIEVCEGDEIVVDVKNHLLGESTSIHWHGLHQKKTPYMDGVPHITQCPITPHATFRYSFPADLSGTHFWHSHTGMQRGDGVFGALIIRKPKTAEPHGGLYDFDLSEHVMIVQDWIHDTGASIFSYHHHSRGDNKPHNLLVNGKGRYYNRIWAEAKQAHRRAEERTTQPVEPLPKSQVDFVQTLPRQARLAKTNTTKLFPVNSRQKRGNLNEIPLELVPHQIYTVRRGFRYRFRIINAEYLNCPIVVSIDGHNLTAINSDGFDIEAMDVGSIVTYSGERFDFVLNANLEVGNYWIRLKGLMDCSEVFTSAFQVAILRYEGAPDEEPTAELSYGHKAEGIELNVMNRGPGYPDTKTVAEMRALPIYDHVSGIDHDTLKPEADYKFFIYYDFYTKNNPDFHDKDLYAMDMEMTQQNRLYTPQLNHITLNFPSLALLPSRSQLKDSDFCNETSLMDQGIDCRQEFCKCHHVLQVPLGAVVEMIIVDEGFQYYANHPFHLHGNAFRVMGLERLGENVTVEMIKQLDQFNLLKRNLDNPPVKDTVTIPDGGYTIIRFEASNPGYWLFHCHIEFHAEIGMALVFKVGNDDQMVPVPENFPTCGDYNPDLRSDGGTTEDSGSSKPITATPPNTGGGGSGLEPTWITLMISSMLVKLYR

Target ORF information:

RefSeq Version	NM_001201825.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster multicopper oxidase 1, transcript variant B (Mco1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201825.2

ORF Insert Sequence:

ATGAGCGTAA GCCATAATTA TGACCAAATG AGCCAGCCAA CAAACACAAC AACAACGCAA 
ACAAAAGCAT CGAAAAGGCC AAGCAGATTC AACAGCAAAA TGCCGGTTGT CAACAGCAGA 
TTAGCAGAGT TAGCTCGGAT AACAGTACTC TTAACAGCAC TGCTTGTGAG CTACGCCAGT 
GGCTTGCAAA CTGTAGGCGA ATCAGCAGAT GATAATACCA TCTGCCTGTA TCGAGAGTAC 
AACACCACTT ATCGAAAGGA GATTGGCGCA GTGTGGTTTG CCTCAAAGGA TCCATGTTTG 
GTGTATTCCT GTGCAGCTGG TGTGGCCAAG GATCCACAGG CGCATATTGT GGCCACCCAA 
GTCGACTGCA ATGAGTTCTA CTGTGAAGTG GGTTCCGAGC TGCGTAGTGT CGAAGGCAGT 
TGCTGCGGGG AGTGTGTAAG GACTCACTGT CAACACAACC ACACTCTGTA TGCGGTGGGT 
GAATCATGGC ACAATGATGC CGATTGCACG CTCATCGAAT GTGGACGTCT GGATAATGGA 
CAGATTGTAA TGAATACATA CAAGAGAAAT TGTCCGGCTT TGACTGAAGA TTGTCCAGCT 
TCAAGATTGG AGGAGCGAAA CTGTTGTCCC TACTGCAGGC CCCTCCAAAC GGCCAGGATT 
GAGGAGCTGC AGGACAATGT AGCGGAGAGC ACGGATGATA TTTGGACAGC CGAATGGTAT 
CGAAACCATC CCTGCAATCG GGATTGTCAA GTGGGAGCCG AACCCATGAC CTGTCGCTAC 
AAGTTCGTGG TGGAATGGTA CCAGACCTTT TCCAAAGCCT GCTACGATTG TCCACGGAAT 
CTTACGGACT GCTCCCGACC ACATTGTGTC ATGGGCGATG GTTTAGAGCG GAGTATCACT 
GTGGTCAATC GTATGATGCC CGGGCCAGCG ATTGAAGTCT GTGAGGGCGA TGAAATAGTG 
GTCGATGTGA AGAATCATTT GTTGGGCGAA AGCACATCGA TTCACTGGCA TGGTCTGCAC 
CAGAAGAAGA CACCCTATAT GGATGGTGTA CCACACATCA CTCAGTGTCC AATTACTCCA 
CATGCGACCT TCAGGTATTC CTTTCCAGCC GATCTCTCCG GCACTCACTT CTGGCATTCG 
CACACTGGCA TGCAGCGAGG TGATGGAGTT TTCGGTGCCT TAATCATCCG GAAACCAAAG 
ACAGCCGAAC CACACGGAGG ACTCTACGAC TTTGACCTCA GCGAGCATGT GATGATTGTC 
CAGGATTGGA TACATGACAC AGGTGCCAGC ATATTTTCCT ATCATCATCA CTCCCGAGGT 
GATAATAAGC CCCACAATCT GCTCGTGAAC GGCAAGGGAC GTTACTATAA TCGCATTTGG 
GCGGAAGCGA AGCAAGCTCA TCGCAGAGCT GAGGAGAGAA CTACCCAACC GGTGGAACCA 
TTGCCCAAGT CCCAGGTGGA CTTCGTTCAG ACACTACCGA GACAAGCTCG ATTGGCTAAA 
ACTAATACAA CCAAACTATT TCCCGTGAAT TCCCGCCAAA AGAGGGGTAA TTTAAATGAG 
ATACCCTTGG AACTAGTTCC CCATCAGATT TACACAGTGA GAAGAGGTTT TCGTTATCGC 
TTCAGAATTA TAAATGCGGA ATATCTAAAC TGTCCTATTG TGGTGTCTAT TGACGGTCAT 
AACTTGACAG CCATAAACTC GGATGGCTTT GATATTGAGG CAATGGACGT GGGCTCTATA 
GTTACCTATT CCGGTGAGCG ATTTGATTTT GTACTAAATG CCAATCTCGA GGTTGGAAAC 
TATTGGATTC GACTAAAGGG TTTGATGGAC TGCAGCGAAG TCTTTACTTC CGCCTTTCAA 
GTGGCCATTC TTCGCTATGA GGGAGCTCCA GATGAAGAGC CTACTGCGGA ATTGAGTTAC 
GGCCACAAGG CTGAGGGCAT TGAACTGAAT GTGATGAACC GCGGTCCTGG TTACCCGGAC 
ACCAAAACCG TTGCAGAAAT GAGGGCTCTG CCCATCTATG ATCATGTCTC GGGCATCGAT 
CACGATACAC TGAAACCGGA AGCGGACTAC AAGTTCTTCA TCTACTACGA TTTCTATACT 
AAGAATAATC CCGATTTTCA CGATAAAGAT CTTTATGCCA TGGACATGGA GATGACCCAG 
CAGAATCGCC TATACACGCC TCAACTGAAT CATATAACCT TAAATTTTCC TTCGCTAGCA 
CTGCTACCTT CCAGGAGTCA GCTAAAGGAC TCGGACTTCT GTAACGAAAC GAGCTTAATG 
GATCAGGGTA TCGATTGTCG CCAGGAGTTC TGCAAGTGTC ATCATGTCCT GCAGGTTCCA 
TTGGGCGCCG TGGTCGAGAT GATCATCGTG GACGAGGGAT TCCAATACTA TGCCAATCAT 
CCCTTTCATT TGCATGGAAA CGCCTTCAGA GTCATGGGAT TGGAGCGTTT GGGCGAAAAT 
GTCACCGTTG AAATGATAAA ACAACTGGAT CAGTTCAATC TACTCAAGCG GAATCTGGAC 
AATCCACCCG TGAAGGACAC AGTTACTATT CCAGATGGAG GCTATACCAT CATCAGATTC 
GAGGCCTCTA ATCCGGGCTA TTGGCTTTTC CATTGCCACA TCGAATTCCA TGCCGAGATC 
GGTATGGCTC TGGTCTTCAA AGTGGGTAAC GACGATCAAA TGGTTCCGGT GCCAGAGAAC 
TTTCCCACCT GCGGCGATTA TAATCCAGAC TTAAGATCTG ATGGAGGAAC TACAGAAGAT 
TCGGGATCCA GCAAGCCGAT AACAGCTACT CCCCCAAATA CAGGAGGCGG TGGATCGGGT 
CTGGAACCCA CTTGGATTAC ATTGATGATT AGTTCCATGC TAGTGAAACT CTATCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mco1 ( NM_001201826.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188755.1 Drosophila melanogaster multicopper oxidase 1, transcript variant C (Mco1), mRNA.

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CloneID	OFa14191
Clone ID Related Accession (Same CDS sequence)	NM_135443.5 , NM_001201825.2 , NM_001201826.2
Accession Version	NM_001201826.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2880bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	multicopper oxidase 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001201826.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGTAA GCCATAATTA TGACCAAATG AGCCAGCCAA CAAACACAAC AACAACGCAA 
ACAAAAGCAT CGAAAAGGCC AAGCAGATTC AACAGCAAAA TGCCGGTTGT CAACAGCAGA 
TTAGCAGAGT TAGCTCGGAT AACAGTACTC TTAACAGCAC TGCTTGTGAG CTACGCCAGT 
GGCTTGCAAA CTGTAGGCGA ATCAGCAGAT GATAATACCA TCTGCCTGTA TCGAGAGTAC 
AACACCACTT ATCGAAAGGA GATTGGCGCA GTGTGGTTTG CCTCAAAGGA TCCATGTTTG 
GTGTATTCCT GTGCAGCTGG TGTGGCCAAG GATCCACAGG CGCATATTGT GGCCACCCAA 
GTCGACTGCA ATGAGTTCTA CTGTGAAGTG GGTTCCGAGC TGCGTAGTGT CGAAGGCAGT 
TGCTGCGGGG AGTGTGTAAG GACTCACTGT CAACACAACC ACACTCTGTA TGCGGTGGGT 
GAATCATGGC ACAATGATGC CGATTGCACG CTCATCGAAT GTGGACGTCT GGATAATGGA 
CAGATTGTAA TGAATACATA CAAGAGAAAT TGTCCGGCTT TGACTGAAGA TTGTCCAGCT 
TCAAGATTGG AGGAGCGAAA CTGTTGTCCC TACTGCAGGC CCCTCCAAAC GGCCAGGATT 
GAGGAGCTGC AGGACAATGT AGCGGAGAGC ACGGATGATA TTTGGACAGC CGAATGGTAT 
CGAAACCATC CCTGCAATCG GGATTGTCAA GTGGGAGCCG AACCCATGAC CTGTCGCTAC 
AAGTTCGTGG TGGAATGGTA CCAGACCTTT TCCAAAGCCT GCTACGATTG TCCACGGAAT 
CTTACGGACT GCTCCCGACC ACATTGTGTC ATGGGCGATG GTTTAGAGCG GAGTATCACT 
GTGGTCAATC GTATGATGCC CGGGCCAGCG ATTGAAGTCT GTGAGGGCGA TGAAATAGTG 
GTCGATGTGA AGAATCATTT GTTGGGCGAA AGCACATCGA TTCACTGGCA TGGTCTGCAC 
CAGAAGAAGA CACCCTATAT GGATGGTGTA CCACACATCA CTCAGTGTCC AATTACTCCA 
CATGCGACCT TCAGGTATTC CTTTCCAGCC GATCTCTCCG GCACTCACTT CTGGCATTCG 
CACACTGGCA TGCAGCGAGG TGATGGAGTT TTCGGTGCCT TAATCATCCG GAAACCAAAG 
ACAGCCGAAC CACACGGAGG ACTCTACGAC TTTGACCTCA GCGAGCATGT GATGATTGTC 
CAGGATTGGA TACATGACAC AGGTGCCAGC ATATTTTCCT ATCATCATCA CTCCCGAGGT 
GATAATAAGC CCCACAATCT GCTCGTGAAC GGCAAGGGAC GTTACTATAA TCGCATTTGG 
GCGGAAGCGA AGCAAGCTCA TCGCAGAGCT GAGGAGAGAA CTACCCAACC GGTGGAACCA 
TTGCCCAAGT CCCAGGTGGA CTTCGTTCAG ACACTACCGA GACAAGCTCG ATTGGCTAAA 
ACTAATACAA CCAAACTATT TCCCGTGAAT TCCCGCCAAA AGAGGGGTAA TTTAAATGAG 
ATACCCTTGG AACTAGTTCC CCATCAGATT TACACAGTGA GAAGAGGTTT TCGTTATCGC 
TTCAGAATTA TAAATGCGGA ATATCTAAAC TGTCCTATTG TGGTGTCTAT TGACGGTCAT 
AACTTGACAG CCATAAACTC GGATGGCTTT GATATTGAGG CAATGGACGT GGGCTCTATA 
GTTACCTATT CCGGTGAGCG ATTTGATTTT GTACTAAATG CCAATCTCGA GGTTGGAAAC 
TATTGGATTC GACTAAAGGG TTTGATGGAC TGCAGCGAAG TCTTTACTTC CGCCTTTCAA 
GTGGCCATTC TTCGCTATGA GGGAGCTCCA GATGAAGAGC CTACTGCGGA ATTGAGTTAC 
GGCCACAAGG CTGAGGGCAT TGAACTGAAT GTGATGAACC GCGGTCCTGG TTACCCGGAC 
ACCAAAACCG TTGCAGAAAT GAGGGCTCTG CCCATCTATG ATCATGTCTC GGGCATCGAT 
CACGATACAC TGAAACCGGA AGCGGACTAC AAGTTCTTCA TCTACTACGA TTTCTATACT 
AAGAATAATC CCGATTTTCA CGATAAAGAT CTTTATGCCA TGGACATGGA GATGACCCAG 
CAGAATCGCC TATACACGCC TCAACTGAAT CATATAACCT TAAATTTTCC TTCGCTAGCA 
CTGCTACCTT CCAGGAGTCA GCTAAAGGAC TCGGACTTCT GTAACGAAAC GAGCTTAATG 
GATCAGGGTA TCGATTGTCG CCAGGAGTTC TGCAAGTGTC ATCATGTCCT GCAGGTTCCA 
TTGGGCGCCG TGGTCGAGAT GATCATCGTG GACGAGGGAT TCCAATACTA TGCCAATCAT 
CCCTTTCATT TGCATGGAAA CGCCTTCAGA GTCATGGGAT TGGAGCGTTT GGGCGAAAAT 
GTCACCGTTG AAATGATAAA ACAACTGGAT CAGTTCAATC TACTCAAGCG GAATCTGGAC 
AATCCACCCG TGAAGGACAC AGTTACTATT CCAGATGGAG GCTATACCAT CATCAGATTC 
GAGGCCTCTA ATCCGGGCTA TTGGCTTTTC CATTGCCACA TCGAATTCCA TGCCGAGATC 
GGTATGGCTC TGGTCTTCAA AGTGGGTAAC GACGATCAAA TGGTTCCGGT GCCAGAGAAC 
TTTCCCACCT GCGGCGATTA TAATCCAGAC TTAAGATCTG ATGGAGGAAC TACAGAAGAT 
TCGGGATCCA GCAAGCCGAT AACAGCTACT CCCCCAAATA CAGGAGGCGG TGGATCGGGT 
CTGGAACCCA CTTGGATTAC ATTGATGATT AGTTCCATGC TAGTGAAACT CTATCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188755.1
CDS	204..3083
Translation	MSVSHNYDQMSQPTNTTTTQTKASKRPSRFNSKMPVVNSRLAELARITVLLTALLVSYASGLQTVGESADDNTICLYREYNTTYRKEIGAVWFASKDPCLVYSCAAGVAKDPQAHIVATQVDCNEFYCEVGSELRSVEGSCCGECVRTHCQHNHTLYAVGESWHNDADCTLIECGRLDNGQIVMNTYKRNCPALTEDCPASRLEERNCCPYCRPLQTARIEELQDNVAESTDDIWTAEWYRNHPCNRDCQVGAEPMTCRYKFVVEWYQTFSKACYDCPRNLTDCSRPHCVMGDGLERSITVVNRMMPGPAIEVCEGDEIVVDVKNHLLGESTSIHWHGLHQKKTPYMDGVPHITQCPITPHATFRYSFPADLSGTHFWHSHTGMQRGDGVFGALIIRKPKTAEPHGGLYDFDLSEHVMIVQDWIHDTGASIFSYHHHSRGDNKPHNLLVNGKGRYYNRIWAEAKQAHRRAEERTTQPVEPLPKSQVDFVQTLPRQARLAKTNTTKLFPVNSRQKRGNLNEIPLELVPHQIYTVRRGFRYRFRIINAEYLNCPIVVSIDGHNLTAINSDGFDIEAMDVGSIVTYSGERFDFVLNANLEVGNYWIRLKGLMDCSEVFTSAFQVAILRYEGAPDEEPTAELSYGHKAEGIELNVMNRGPGYPDTKTVAEMRALPIYDHVSGIDHDTLKPEADYKFFIYYDFYTKNNPDFHDKDLYAMDMEMTQQNRLYTPQLNHITLNFPSLALLPSRSQLKDSDFCNETSLMDQGIDCRQEFCKCHHVLQVPLGAVVEMIIVDEGFQYYANHPFHLHGNAFRVMGLERLGENVTVEMIKQLDQFNLLKRNLDNPPVKDTVTIPDGGYTIIRFEASNPGYWLFHCHIEFHAEIGMALVFKVGNDDQMVPVPENFPTCGDYNPDLRSDGGTTEDSGSSKPITATPPNTGGGGSGLEPTWITLMISSMLVKLYR

Target ORF information:

RefSeq Version	NM_001201826.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster multicopper oxidase 1, transcript variant C (Mco1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201826.2

ORF Insert Sequence:

ATGAGCGTAA GCCATAATTA TGACCAAATG AGCCAGCCAA CAAACACAAC AACAACGCAA 
ACAAAAGCAT CGAAAAGGCC AAGCAGATTC AACAGCAAAA TGCCGGTTGT CAACAGCAGA 
TTAGCAGAGT TAGCTCGGAT AACAGTACTC TTAACAGCAC TGCTTGTGAG CTACGCCAGT 
GGCTTGCAAA CTGTAGGCGA ATCAGCAGAT GATAATACCA TCTGCCTGTA TCGAGAGTAC 
AACACCACTT ATCGAAAGGA GATTGGCGCA GTGTGGTTTG CCTCAAAGGA TCCATGTTTG 
GTGTATTCCT GTGCAGCTGG TGTGGCCAAG GATCCACAGG CGCATATTGT GGCCACCCAA 
GTCGACTGCA ATGAGTTCTA CTGTGAAGTG GGTTCCGAGC TGCGTAGTGT CGAAGGCAGT 
TGCTGCGGGG AGTGTGTAAG GACTCACTGT CAACACAACC ACACTCTGTA TGCGGTGGGT 
GAATCATGGC ACAATGATGC CGATTGCACG CTCATCGAAT GTGGACGTCT GGATAATGGA 
CAGATTGTAA TGAATACATA CAAGAGAAAT TGTCCGGCTT TGACTGAAGA TTGTCCAGCT 
TCAAGATTGG AGGAGCGAAA CTGTTGTCCC TACTGCAGGC CCCTCCAAAC GGCCAGGATT 
GAGGAGCTGC AGGACAATGT AGCGGAGAGC ACGGATGATA TTTGGACAGC CGAATGGTAT 
CGAAACCATC CCTGCAATCG GGATTGTCAA GTGGGAGCCG AACCCATGAC CTGTCGCTAC 
AAGTTCGTGG TGGAATGGTA CCAGACCTTT TCCAAAGCCT GCTACGATTG TCCACGGAAT 
CTTACGGACT GCTCCCGACC ACATTGTGTC ATGGGCGATG GTTTAGAGCG GAGTATCACT 
GTGGTCAATC GTATGATGCC CGGGCCAGCG ATTGAAGTCT GTGAGGGCGA TGAAATAGTG 
GTCGATGTGA AGAATCATTT GTTGGGCGAA AGCACATCGA TTCACTGGCA TGGTCTGCAC 
CAGAAGAAGA CACCCTATAT GGATGGTGTA CCACACATCA CTCAGTGTCC AATTACTCCA 
CATGCGACCT TCAGGTATTC CTTTCCAGCC GATCTCTCCG GCACTCACTT CTGGCATTCG 
CACACTGGCA TGCAGCGAGG TGATGGAGTT TTCGGTGCCT TAATCATCCG GAAACCAAAG 
ACAGCCGAAC CACACGGAGG ACTCTACGAC TTTGACCTCA GCGAGCATGT GATGATTGTC 
CAGGATTGGA TACATGACAC AGGTGCCAGC ATATTTTCCT ATCATCATCA CTCCCGAGGT 
GATAATAAGC CCCACAATCT GCTCGTGAAC GGCAAGGGAC GTTACTATAA TCGCATTTGG 
GCGGAAGCGA AGCAAGCTCA TCGCAGAGCT GAGGAGAGAA CTACCCAACC GGTGGAACCA 
TTGCCCAAGT CCCAGGTGGA CTTCGTTCAG ACACTACCGA GACAAGCTCG ATTGGCTAAA 
ACTAATACAA CCAAACTATT TCCCGTGAAT TCCCGCCAAA AGAGGGGTAA TTTAAATGAG 
ATACCCTTGG AACTAGTTCC CCATCAGATT TACACAGTGA GAAGAGGTTT TCGTTATCGC 
TTCAGAATTA TAAATGCGGA ATATCTAAAC TGTCCTATTG TGGTGTCTAT TGACGGTCAT 
AACTTGACAG CCATAAACTC GGATGGCTTT GATATTGAGG CAATGGACGT GGGCTCTATA 
GTTACCTATT CCGGTGAGCG ATTTGATTTT GTACTAAATG CCAATCTCGA GGTTGGAAAC 
TATTGGATTC GACTAAAGGG TTTGATGGAC TGCAGCGAAG TCTTTACTTC CGCCTTTCAA 
GTGGCCATTC TTCGCTATGA GGGAGCTCCA GATGAAGAGC CTACTGCGGA ATTGAGTTAC 
GGCCACAAGG CTGAGGGCAT TGAACTGAAT GTGATGAACC GCGGTCCTGG TTACCCGGAC 
ACCAAAACCG TTGCAGAAAT GAGGGCTCTG CCCATCTATG ATCATGTCTC GGGCATCGAT 
CACGATACAC TGAAACCGGA AGCGGACTAC AAGTTCTTCA TCTACTACGA TTTCTATACT 
AAGAATAATC CCGATTTTCA CGATAAAGAT CTTTATGCCA TGGACATGGA GATGACCCAG 
CAGAATCGCC TATACACGCC TCAACTGAAT CATATAACCT TAAATTTTCC TTCGCTAGCA 
CTGCTACCTT CCAGGAGTCA GCTAAAGGAC TCGGACTTCT GTAACGAAAC GAGCTTAATG 
GATCAGGGTA TCGATTGTCG CCAGGAGTTC TGCAAGTGTC ATCATGTCCT GCAGGTTCCA 
TTGGGCGCCG TGGTCGAGAT GATCATCGTG GACGAGGGAT TCCAATACTA TGCCAATCAT 
CCCTTTCATT TGCATGGAAA CGCCTTCAGA GTCATGGGAT TGGAGCGTTT GGGCGAAAAT 
GTCACCGTTG AAATGATAAA ACAACTGGAT CAGTTCAATC TACTCAAGCG GAATCTGGAC 
AATCCACCCG TGAAGGACAC AGTTACTATT CCAGATGGAG GCTATACCAT CATCAGATTC 
GAGGCCTCTA ATCCGGGCTA TTGGCTTTTC CATTGCCACA TCGAATTCCA TGCCGAGATC 
GGTATGGCTC TGGTCTTCAA AGTGGGTAAC GACGATCAAA TGGTTCCGGT GCCAGAGAAC 
TTTCCCACCT GCGGCGATTA TAATCCAGAC TTAAGATCTG ATGGAGGAAC TACAGAAGAT 
TCGGGATCCA GCAAGCCGAT AACAGCTACT CCCCCAAATA CAGGAGGCGG TGGATCGGGT 
CTGGAACCCA CTTGGATTAC ATTGATGATT AGTTCCATGC TAGTGAAACT CTATCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Mco1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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