Dh31 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dh31
Entrez Gene ID	34181
Full Name	Diuretic hormone 31
Synonyms	CG13094,DH 31,DH-II,DH31,DH[[31],DH[[31]],Dh[[31]],Dmel\CG13094,Drm-DH[[31]],Drome-DH[[31]],anon-EST:Posey114,anon-WO02059370.45,dh31,drome-DH31
General protein information	Preferred Names Diuretic hormone 31 Names CG13094 gene product from transcript CG13094-RA CG13094-PA CG13094-PC Calcitonin-like diuretic hormone (31 residues) Dh31-PA Dh31-PC calcitonin diuretic hormone diuretic hormone 31
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 29D1-29D3

mRNA and Protein(s)

mRNA	Protein	Name
NM_078790.3	NP_523514.1	diuretic hormone 31, isoform A
NM_164825.2	NP_723401.1	diuretic hormone 31, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Body temperature rhythm (BTR) is fundamental for the maintenance of functions essential for homeostasis, such as generating metabolic energy and sleep. One major unsolved question is how body temperature decreases dramatically during the night. Previously, we demonstrated that a BTR-like mechanism, referred to as temperature preference rhythm (TPR), exists in Drosophila Here, we demonstrate that the diuretic hormone 31 (D
Title: Drosophila DH31 Neuropeptide and PDF Receptor Regulate Night-Onset Temperature Preference.

the Midgut Junction DH31 expressing cells are necessary and sufficient for motility and that the peptide hormone DH31 is required for peristalsis in the junction region of the midgut.
Title: Peristalsis in the junction region of the Drosophila larval midgut is modulated by DH31 expressing enteroendocrine cells.

The following Dh31 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dh31 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa16484	NM_078790.3 Latest version!	Drosophila melanogaster diuretic hormone 31, transcript variant A (Dh31), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa16485	NM_164825.2 Latest version!	Drosophila melanogaster diuretic hormone 31, transcript variant C (Dh31), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dh31 ( NM_078790.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523514.1 Drosophila melanogaster diuretic hormone 31, transcript variant A (Dh31), mRNA.

Price & Availability↑

CloneID	OFa16484
Clone ID Related Accession (Same CDS sequence)	NM_078790.3
Accession Version	NM_078790.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	diuretic hormone 31, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Dec 17, 2009 this sequence version replaced NM_078790.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAAACC GATGCGCTTG CTTCGCCTTG GCCTTCCTCC TCTTCTGCCT CTTGGCCATC 
TCGAGCATCG AGGCAGCTCC GATGCCCAGC CAATCCAATG GAGGATACGG TGGCGCTGGC 
TATAACGAAC TGGAGGAGGT GCCCGACGAC CTACTCATGG AACTGATGAC TCGCTTTGGA 
CGCACCATCA TACGGGCTCG AAACGATCTG GAGAATTCCA AACGAACCGT GGACTTTGGC 
TTGGCCCGGG GATATTCGGG TACCCAGGAG GCGAAACATC GCATGGGTCT GGCTGCAGCC 
AACTTTGCCG GAGGACCCGG TCGGAGGCGA CGATCCGAGA CCGATGTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523514.1
CDS	143..493
Translation	MTNRCACFALAFLLFCLLAISSIEAAPMPSQSNGGYGGAGYNELEEVPDDLLMELMTRFGRTIIRARNDLENSKRTVDFGLARGYSGTQEAKHRMGLAAANFAGGPGRRRRSETDV

Target ORF information:

RefSeq Version	NM_078790.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster diuretic hormone 31, transcript variant A (Dh31), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078790.3

ORF Insert Sequence:

ATGACAAACC GATGCGCTTG CTTCGCCTTG GCCTTCCTCC TCTTCTGCCT CTTGGCCATC 
TCGAGCATCG AGGCAGCTCC GATGCCCAGC CAATCCAATG GAGGATACGG TGGCGCTGGC 
TATAACGAAC TGGAGGAGGT GCCCGACGAC CTACTCATGG AACTGATGAC TCGCTTTGGA 
CGCACCATCA TACGGGCTCG AAACGATCTG GAGAATTCCA AACGAACCGT GGACTTTGGC 
TTGGCCCGGG GATATTCGGG TACCCAGGAG GCGAAACATC GCATGGGTCT GGCTGCAGCC 
AACTTTGCCG GAGGACCCGG TCGGAGGCGA CGATCCGAGA CCGATGTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dh31 ( NM_164825.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723401.1 Drosophila melanogaster diuretic hormone 31, transcript variant C (Dh31), mRNA.

Price & Availability↑

CloneID	OFa16485
Clone ID Related Accession (Same CDS sequence)	NM_164825.2
Accession Version	NM_164825.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	diuretic hormone 31, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Dec 17, 2009 this sequence version replaced NM_164825.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAAACC GATGCGCTTG CTTCGCCTTG GCCTTCCTCC TCTTCTGCCT CTTGGCCATC 
TCGAGCATCG AGGCAGCTCC GATGCCCAGG TACCAATCCA ATGGAGGATA CGGTGGCGCT 
GGCTATAACG AACTGGAGGA GGTGCCCGAC GACCTACTCA TGGAACTGAT GACTCGCTTT 
GGACGCACCA TCATACGGGC TCGAAACGAT CTGGAGAATT CCAAACGAAC CGTGGACTTT 
GGCTTGGCCC GGGGATATTC GGGTACCCAG GAGGCGAAAC ATCGCATGGG TCTGGCTGCA 
GCCAACTTTG CCGGAGGACC CGGTCGGAGG CGACGATCCG AGACCGATGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723401.1
CDS	143..496
Translation	MTNRCACFALAFLLFCLLAISSIEAAPMPRYQSNGGYGGAGYNELEEVPDDLLMELMTRFGRTIIRARNDLENSKRTVDFGLARGYSGTQEAKHRMGLAAANFAGGPGRRRRSETDV

Target ORF information:

RefSeq Version	NM_164825.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster diuretic hormone 31, transcript variant C (Dh31), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164825.2

ORF Insert Sequence:

ATGACAAACC GATGCGCTTG CTTCGCCTTG GCCTTCCTCC TCTTCTGCCT CTTGGCCATC 
TCGAGCATCG AGGCAGCTCC GATGCCCAGG TACCAATCCA ATGGAGGATA CGGTGGCGCT 
GGCTATAACG AACTGGAGGA GGTGCCCGAC GACCTACTCA TGGAACTGAT GACTCGCTTT 
GGACGCACCA TCATACGGGC TCGAAACGAT CTGGAGAATT CCAAACGAAC CGTGGACTTT 
GGCTTGGCCC GGGGATATTC GGGTACCCAG GAGGCGAAAC ATCGCATGGG TCTGGCTGCA 
GCCAACTTTG CCGGAGGACC CGGTCGGAGG CGACGATCCG AGACCGATGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Dh31 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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