CG8419 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG8419
Entrez Gene ID	34118
Full Name	CG8419 gene product from transcript CG8419-RA
Synonyms	Dmel\CG8419
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 28F1-28F2

mRNA and Protein(s)

mRNA	Protein	Name
NM_135349.3	NP_609193.1	uncharacterized protein

Pathways from BioSystems

Homologies

	TRIM45	XP_001113153.1
Rattus norvegicus (Norway rat)	Trim45	NP_001099923.1
Danio rerio (zebrafish)	trim45	XP_686393.2
Pan troglodytes (chimpanzee)	TRIM45	XP_001145514.1
Homo sapiens (human)	TRIM45	NP_079464.2
Canis lupus familiaris (dog)	TRIM45	XP_533020.2
Gallus gallus (chicken)	TRIM45	XP_004938219.1
Drosophila melanogaster (fruit fly)	CG8419	NP_609193.1
Bos taurus (cattle)	TRIM45	NP_001014952.1
Mus musculus (house mouse)	Trim45	NP_919324.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG8419 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG8419 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14098	NM_135349.3 Latest version!	Drosophila melanogaster uncharacterized protein (CG8419), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG8419 ( NM_135349.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609193.1 Drosophila melanogaster uncharacterized protein (CG8419), mRNA.

Price & Availability↑

CloneID	OFa14098
Clone ID Related Accession (Same CDS sequence)	NM_135349.3
Accession Version	NM_135349.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_135349.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCAGGG AAAATCGCTC GAAAATCGCT TTCAAGCGAA AAACGAGTAA CGGAAACAAG 
AGTGGCCAGT TAACGGATTA CGAACGCATC CGAGGGGATG TGGAAATAGT TGTTCCGAGC 
CCACCTCCTA CTCCAGCTGT GCCACCCAGT GCATCCTTAG GGAAACTTAA GATAAAGTTG 
AGTTCTGCCA AAATTTCCTT ACGAAAAAAC GCACAGACTT CGCAGAATAA TCTGCAGACC 
GCTCCCTTAC CAGACGCAGT GCGTCGCAAA TCAGCACCAC AACTTTTTAC CTTTACTATT 
GCCCCAAAGA GCGAAGTGAC TTCGAAAATA CATCTACCCT CAGTGCCAAA GCTACCGGGT 
TTGGCACCAA AGAGCTCCAT CCGAAGATCT AGGACTTTGG GTTTGTCGAA TAGTGCCCAG 
GGTGCAGGGT CATCAGGTAC AGGGCTATCC AATGTCGAAC TCGGTGTGCA GGATAGGGAC 
AGTGGCAGTG AGCCCACTAG CAGCTTGGCT GGAGGAGGTA GCTCTACTTC GCCTGAATCC 
CTTTTGGATA ACATACTCAG CGGTGCCTCC TCCGTTTCCG TACAAAGTAG TAGCAGCAGC 
CAAGCCAGTA ATGCTACGGT GGCACAGCCC ATAAAAGCAA AGGAGCAACT GCATCCCAAG 
AGGCTACTCA GCTTGAAGGC TTCTCCGGAG CTGAGAGTAT CTCCACCAAC TCCAGACACT 
ACTCAACCCC AGGTCCAGAT GCCAACTCAA CTTCTGCTTT CACTGACTCC AAGACCAGAG 
GTCTTTGATG CACCCTCACC ACTGACCAGA AGTCCCTCAC GATCTCCCGG AATCTCTCCC 
CCCGTGTCAC CATCACCCAG CATTACTCTC AGACCGAGTA CCCCTCCCGA GAACACCGTA 
ATCTTCACAG ATGATCTCAA GTGCGGCATC TGCCTGGATG TTTACACAGA TCCCAGGACA 
CTGCATTGCT TGCATTCCTT TTGTCTCCAA TGTCTGGTCA GCGAGAACTT TAAGGACGAA 
TCTTTGTGGG ATCAGGATCA AGCTCGCAGT GAGGAGCCCT CGAATTACAG TCTAAGATCG 
GAGATGGGTG GATCTAGTGC GGAACTTACT GCGACAGTAT CACCCGCCAG GCAGCGAGGT 
TCCAGTTTTA CATTGAGAAG AAAGAAGTCC ATGGATCGCC TAGTGATAAG GTCCAAGAGT 
GACGGAAAGC GATCGACCAG TTCCATCGGT ACCCGATTCA CCGGAAGTTT TGCCAGCGAG 
GTATCCACCC GCTGCATCCG CTGTCATACC TGTAACTATC CCACGGATCT TTCGTTAGGT 
GGAGTACGTC AGCTTCCACA GAACTACTTG CTAGTGAGAC GCATAGAGGT CTTGCGACTA 
CAGGCTGGGG AGGATGTAAT CTCCAGGGTC TGGTGTTCTC TTTGCACAGA GGAGATTAGC 
GCCACCTACC ACTGCATTAG CTGCACTTTA AACCTCTGCA CCTTGTGCAA GGAGGCACAC 
GAAAGGCAAA GGAGCACCGC TAGCCATCGA ATGAGAAGCA TCCTGGAACT TAGACGCGCC 
AGGAAGCAAA AGCAACAGCA GATGGGATTG GGAGATAGTA GCAAACTAGT GCTACGATGC 
GGAATCCATA CCAACTTTGA GCTGAAGGCA TTTTGCACCA ACTGCCGTCA ACTGGCATGC 
ACCGACTGTT TGGTGATCCT TCATAAAGGC CATCGACATG AGACCATATC CCGGGCAATT 
GGACATCAGG GTAAACTACT CAAAGAAGCT ACGGATCAAA CTCGTCCCCT CTGTCAATAT 
GCGGAGCACT CCATTGAGAG GTTGAATGCC ATAGCACGTG GAATCAACGA CCGGTGTGAC 
GACATCCAGA CTCAAGTGGA GCGGTACATG CAGCAGTACA TGGATGCCCT GACAGTCCAT 
CGCAAGACTC TGCTCCAGCA AATCAGCAGA GCTAGGGAGT CCAAAGTGGA GGTAGTGCTC 
AAGCAGCAAC TTGATTTGGA GAAACGCACT CAGCAGGCCA TGGATGCGGT TAGATTTAGC 
CAAGAGCTGT GCGAGATTGG TGCTGATGTA GAGATTCTGA GTTATGTGAC TATACTTCTA 
AGGCGTTTGG AGTACTGTCA GCAATTTAAG CCGCCTGTGG ATCCCAAGAT ATCCGACTCG 
CTTCACTTCC TACCAAAAAT CCGTGCTCCG TCGACAAAGG ACCAGCGCGA TATCCCTCTA 
TATGGCATCA TCACCATGCA GGTAGTGGAG CCTAGTCTAT GCACCCTTGA GTGGGAGGGA 
TTTTCACAGC TGCGCCTCCA CAAAAAAGCG GATCTTCTGC TTCACTCTAG AGATGCCGAC 
GGGGTTTCCC TCTGCCACGG TGGCCTGGAG ATCAACTGCA TGCTGAAGTA CAAGGACTCC 
AGTTCCAAGT TTCTACCCGT TGAGGTGTCG GACAATCGTG ATGGCACCTA CAACATATCC 
TTCACCCCGG ATGCCCAGGG CACGTTGATT CTCACGATCA CCATTAACGA CCGGCCCATC 
AAGGGAGGTC CTTTCACTTT TCAAGCGCGA CAGGTTCGTC CGCATACGGG CATCTACCAC 
TGCTGCTCCT TCTGTTCCGG AAAGGGCAAC CGGAACGTGA TGTGCTCGTG TGAGGGTCAT 
ATGCCTGGAT ATAGTGGCTG CGGTCATGGA CATGCTGGAC ATCCAGGGAG ACGTCATTGG 
TCCTGCTGTG GAAATGTCCT TGAGAACTCG GAATGCAATG TGGCCAACAA ACTGCTTAAT 
TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609193.1
CDS	109..2874
Translation	MSRENRSKIAFKRKTSNGNKSGQLTDYERIRGDVEIVVPSPPPTPAVPPSASLGKLKIKLSSAKISLRKNAQTSQNNLQTAPLPDAVRRKSAPQLFTFTIAPKSEVTSKIHLPSVPKLPGLAPKSSIRRSRTLGLSNSAQGAGSSGTGLSNVELGVQDRDSGSEPTSSLAGGGSSTSPESLLDNILSGASSVSVQSSSSSQASNATVAQPIKAKEQLHPKRLLSLKASPELRVSPPTPDTTQPQVQMPTQLLLSLTPRPEVFDAPSPLTRSPSRSPGISPPVSPSPSITLRPSTPPENTVIFTDDLKCGICLDVYTDPRTLHCLHSFCLQCLVSENFKDESLWDQDQARSEEPSNYSLRSEMGGSSAELTATVSPARQRGSSFTLRRKKSMDRLVIRSKSDGKRSTSSIGTRFTGSFASEVSTRCIRCHTCNYPTDLSLGGVRQLPQNYLLVRRIEVLRLQAGEDVISRVWCSLCTEEISATYHCISCTLNLCTLCKEAHERQRSTASHRMRSILELRRARKQKQQQMGLGDSSKLVLRCGIHTNFELKAFCTNCRQLACTDCLVILHKGHRHETISRAIGHQGKLLKEATDQTRPLCQYAEHSIERLNAIARGINDRCDDIQTQVERYMQQYMDALTVHRKTLLQQISRARESKVEVVLKQQLDLEKRTQQAMDAVRFSQELCEIGADVEILSYVTILLRRLEYCQQFKPPVDPKISDSLHFLPKIRAPSTKDQRDIPLYGIITMQVVEPSLCTLEWEGFSQLRLHKKADLLLHSRDADGVSLCHGGLEINCMLKYKDSSSKFLPVEVSDNRDGTYNISFTPDAQGTLILTITINDRPIKGGPFTFQARQVRPHTGIYHCCSFCSGKGNRNVMCSCEGHMPGYSGCGHGHAGHPGRRHWSCCGNVLENSECNVANKLLNS

Target ORF information:

RefSeq Version	NM_135349.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein (CG8419), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135349.3

ORF Insert Sequence:

ATGAGCAGGG AAAATCGCTC GAAAATCGCT TTCAAGCGAA AAACGAGTAA CGGAAACAAG 
AGTGGCCAGT TAACGGATTA CGAACGCATC CGAGGGGATG TGGAAATAGT TGTTCCGAGC 
CCACCTCCTA CTCCAGCTGT GCCACCCAGT GCATCCTTAG GGAAACTTAA GATAAAGTTG 
AGTTCTGCCA AAATTTCCTT ACGAAAAAAC GCACAGACTT CGCAGAATAA TCTGCAGACC 
GCTCCCTTAC CAGACGCAGT GCGTCGCAAA TCAGCACCAC AACTTTTTAC CTTTACTATT 
GCCCCAAAGA GCGAAGTGAC TTCGAAAATA CATCTACCCT CAGTGCCAAA GCTACCGGGT 
TTGGCACCAA AGAGCTCCAT CCGAAGATCT AGGACTTTGG GTTTGTCGAA TAGTGCCCAG 
GGTGCAGGGT CATCAGGTAC AGGGCTATCC AATGTCGAAC TCGGTGTGCA GGATAGGGAC 
AGTGGCAGTG AGCCCACTAG CAGCTTGGCT GGAGGAGGTA GCTCTACTTC GCCTGAATCC 
CTTTTGGATA ACATACTCAG CGGTGCCTCC TCCGTTTCCG TACAAAGTAG TAGCAGCAGC 
CAAGCCAGTA ATGCTACGGT GGCACAGCCC ATAAAAGCAA AGGAGCAACT GCATCCCAAG 
AGGCTACTCA GCTTGAAGGC TTCTCCGGAG CTGAGAGTAT CTCCACCAAC TCCAGACACT 
ACTCAACCCC AGGTCCAGAT GCCAACTCAA CTTCTGCTTT CACTGACTCC AAGACCAGAG 
GTCTTTGATG CACCCTCACC ACTGACCAGA AGTCCCTCAC GATCTCCCGG AATCTCTCCC 
CCCGTGTCAC CATCACCCAG CATTACTCTC AGACCGAGTA CCCCTCCCGA GAACACCGTA 
ATCTTCACAG ATGATCTCAA GTGCGGCATC TGCCTGGATG TTTACACAGA TCCCAGGACA 
CTGCATTGCT TGCATTCCTT TTGTCTCCAA TGTCTGGTCA GCGAGAACTT TAAGGACGAA 
TCTTTGTGGG ATCAGGATCA AGCTCGCAGT GAGGAGCCCT CGAATTACAG TCTAAGATCG 
GAGATGGGTG GATCTAGTGC GGAACTTACT GCGACAGTAT CACCCGCCAG GCAGCGAGGT 
TCCAGTTTTA CATTGAGAAG AAAGAAGTCC ATGGATCGCC TAGTGATAAG GTCCAAGAGT 
GACGGAAAGC GATCGACCAG TTCCATCGGT ACCCGATTCA CCGGAAGTTT TGCCAGCGAG 
GTATCCACCC GCTGCATCCG CTGTCATACC TGTAACTATC CCACGGATCT TTCGTTAGGT 
GGAGTACGTC AGCTTCCACA GAACTACTTG CTAGTGAGAC GCATAGAGGT CTTGCGACTA 
CAGGCTGGGG AGGATGTAAT CTCCAGGGTC TGGTGTTCTC TTTGCACAGA GGAGATTAGC 
GCCACCTACC ACTGCATTAG CTGCACTTTA AACCTCTGCA CCTTGTGCAA GGAGGCACAC 
GAAAGGCAAA GGAGCACCGC TAGCCATCGA ATGAGAAGCA TCCTGGAACT TAGACGCGCC 
AGGAAGCAAA AGCAACAGCA GATGGGATTG GGAGATAGTA GCAAACTAGT GCTACGATGC 
GGAATCCATA CCAACTTTGA GCTGAAGGCA TTTTGCACCA ACTGCCGTCA ACTGGCATGC 
ACCGACTGTT TGGTGATCCT TCATAAAGGC CATCGACATG AGACCATATC CCGGGCAATT 
GGACATCAGG GTAAACTACT CAAAGAAGCT ACGGATCAAA CTCGTCCCCT CTGTCAATAT 
GCGGAGCACT CCATTGAGAG GTTGAATGCC ATAGCACGTG GAATCAACGA CCGGTGTGAC 
GACATCCAGA CTCAAGTGGA GCGGTACATG CAGCAGTACA TGGATGCCCT GACAGTCCAT 
CGCAAGACTC TGCTCCAGCA AATCAGCAGA GCTAGGGAGT CCAAAGTGGA GGTAGTGCTC 
AAGCAGCAAC TTGATTTGGA GAAACGCACT CAGCAGGCCA TGGATGCGGT TAGATTTAGC 
CAAGAGCTGT GCGAGATTGG TGCTGATGTA GAGATTCTGA GTTATGTGAC TATACTTCTA 
AGGCGTTTGG AGTACTGTCA GCAATTTAAG CCGCCTGTGG ATCCCAAGAT ATCCGACTCG 
CTTCACTTCC TACCAAAAAT CCGTGCTCCG TCGACAAAGG ACCAGCGCGA TATCCCTCTA 
TATGGCATCA TCACCATGCA GGTAGTGGAG CCTAGTCTAT GCACCCTTGA GTGGGAGGGA 
TTTTCACAGC TGCGCCTCCA CAAAAAAGCG GATCTTCTGC TTCACTCTAG AGATGCCGAC 
GGGGTTTCCC TCTGCCACGG TGGCCTGGAG ATCAACTGCA TGCTGAAGTA CAAGGACTCC 
AGTTCCAAGT TTCTACCCGT TGAGGTGTCG GACAATCGTG ATGGCACCTA CAACATATCC 
TTCACCCCGG ATGCCCAGGG CACGTTGATT CTCACGATCA CCATTAACGA CCGGCCCATC 
AAGGGAGGTC CTTTCACTTT TCAAGCGCGA CAGGTTCGTC CGCATACGGG CATCTACCAC 
TGCTGCTCCT TCTGTTCCGG AAAGGGCAAC CGGAACGTGA TGTGCTCGTG TGAGGGTCAT 
ATGCCTGGAT ATAGTGGCTG CGGTCATGGA CATGCTGGAC ATCCAGGGAG ACGTCATTGG 
TCCTGCTGTG GAAATGTCCT TGAGAACTCG GAATGCAATG TGGCCAACAA ACTGCTTAAT 
TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG8419 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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