spz3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	spz3
Entrez Gene ID	34077
Full Name	spatzle 3
Synonyms	CG14533,CG7104,CT21967,CT34263,Dmel\CG7104,Spz3
General protein information	Preferred Names spatzle 3 Names CG7104 gene product from transcript CG7104-RA CG7104-PA Spz3 spz3-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 28D2-28D2

mRNA and Protein(s)

mRNA	Protein	Name
NM_135316.3	NP_609160.2	spatzle 3

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	spz3	NP_609160.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008360	XP_317093.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Spz3 is a novel signaling factor required for maintenance of cortex glial morphology and neuron-glia contact.
Title: The secreted neurotrophin Sp?tzle 3 promotes glial morphogenesis and supports neuronal survival and function.

Spz3 expression in muscle and proteolytic activation via the Easter protease is necessary and sufficient to promote NMJ growth.
Title: Retrograde neurotrophin signaling through Tollo regulates synaptic growth in Drosophila.

The following spz3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spz3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa16362	NM_135316.3 Latest version!	Drosophila melanogaster spatzle 3 (spz3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

spz3 ( NM_135316.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609160.2 Drosophila melanogaster spatzle 3 (spz3), mRNA.

Price & Availability↑

CloneID	OFa16362
Clone ID Related Accession (Same CDS sequence)	NM_135316.3
Accession Version	NM_135316.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1836bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spatzle 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_135316.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTCTGA CCAATTTCTC GCTGCCCTTC GGTGCCCTGG GCCAGCCCTG GGGAGTCACC 
ATAGCTCCCC TGCATCCAAT CCACCAGTTG GCCAGCAACA CAAATAATCT GCTCTACTCG 
CCAGCTGACC ACCAGCAGCA GACTCCCGCG GAGGCAGCCG CCGATCCGGA GTACTTCAAG 
AACAATCCCT ATGCGCCGCC GCAATCGGGT GGTTATCAAT ATCAAAATAC AGCTGGACGA 
CGCAAGCAGA GTAACGCCTA TTTGCCACCG ACAGCGCCGA ATGCTGTCCG GAATTCCGTT 
TACCACATCC AGCAGGTACA GCAGACGCAG CAGCAGCAAA CGCAACAACA ACACCAGCAG 
CAGGATCAGC ATGAGAACAG CGTGTCCTTC CAGAGCAGCA GTTCCAGATC AAGTTCGAGC 
AGCACCACGG GCCAAAGTTC CATCCAGCTG ACACAGACTC ATGCGAGTGG CCGGGGACCG 
GCGGAGGGTT CGTACTCCCG TTATCCCGGA CAGCAAGCAC AGCCGCCGCA GCAGCAGCAA 
CCGCAACAGA AGCAATATTT CAATGCACAC GGCAGTGCCA GTGCCACTTT TACTAAGAAC 
AGCGGCAGCT TTAGTATTAC CAGCTTTGGC AGCAGGCAGC AGCAGCAGCA GCCACCGCAG 
CCGCAACAAC CACCGCCATC GCAACAGCAG CAACCACCAC CTGCACCACC GCCCCAAAGG 
TCCAGGCAGG CCAAGCCGGA GGCTCAACCA GCTCAGACAT ACGGAGTTGC CCCGCCGGAG 
AATTATCCGG AACGAGCACC AGGATTTACA CGGGTTCAGG CTGGTCAGGG ATCCAGGACT 
CAGGTACACG CCGTTCTAGA CTACGACGTG GAGGAGGGTG AGGAGGATGA GGAGGAGGAC 
GGAGAGGAGG AAGGACAGTT CTACGAAGGC CAAGAGAATG ATAAGTCGAA TAACAATCAG 
ATGCCCACAG TGACGCCCAT CCAGGGACCG ATTTACTTGA AGAACGGGAC GGTTCCAGTG 
GTGCCGCTCT TCTCCTATCC GAAACTCAAC AACGGATCGT TCCTACAGAT TCCGATCTGG 
TGGACGGCTT TGTCGGTGGC TTTGGGACTG GATGTGCGCG GCGATGTCAT CAAGGGAGTG 
CCGTGCATCA AGCGATATCA TCAACTGTTT TGCCCCACGG CCGGCAATAG TTATCCCATT 
GACAAGATTG AACGTTTCAT AGACGACAAC AAGGCCCTGA TGCGACGGAT GTACGGCGAC 
TTTGAGATGA ACATGGAGGG TCCTGGCGGA GGAGGTGGCA GACAACAAGG CAAGGTGCGA 
AAGCGTCGCT TCATAGACGA GCCGGATATA TTCATACCCC CGGGAGCATT TGCTGCCAAT 
GCCGGGGAGA CCGTCGAAGC CGGAGATAGT TACTTTGGCC AACTCCGGAA AAAGCGCCAA 
GCTGCCGCCG GAGGAAGTCG AAATAGAGGC GGATCTGCCG GAGGAAGTGG CAATGGGAAC 
ACGAATGCCA ATAGGCAACC GGGAAATAAG AATGGTAGTT CGGGCACTGG AAGACTCGAT 
GCCTGCGAGT CGAAGATTGA AATTGTCACA CCGTACTGGG CATCGAACTC CGCGGGTAAG 
ATCAGAGCCA TCGTAAATAC TCAGCATTTC GAGCAGGCGA TTCACCAGGA GGTTTGCAGC 
AATACCCAAA CTCCAAGATG TGAGGGCGAG TGTGGATGCG AGCAGAAATA CAAATGGCAT 
AGATTACTCG CCTACGATCC TGATAACGAT TGCAAGGGCA TTTTTATGGA TTGGTTCCTG 
TTTCCTTCGT GCTGTGTCTG TAGATGTAAT CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609160.2
CDS	407..2242
Translation	MALTNFSLPFGALGQPWGVTIAPLHPIHQLASNTNNLLYSPADHQQQTPAEAAADPEYFKNNPYAPPQSGGYQYQNTAGRRKQSNAYLPPTAPNAVRNSVYHIQQVQQTQQQQTQQQHQQQDQHENSVSFQSSSSRSSSSSTTGQSSIQLTQTHASGRGPAEGSYSRYPGQQAQPPQQQQPQQKQYFNAHGSASATFTKNSGSFSITSFGSRQQQQQPPQPQQPPPSQQQQPPPAPPPQRSRQAKPEAQPAQTYGVAPPENYPERAPGFTRVQAGQGSRTQVHAVLDYDVEEGEEDEEEDGEEEGQFYEGQENDKSNNNQMPTVTPIQGPIYLKNGTVPVVPLFSYPKLNNGSFLQIPIWWTALSVALGLDVRGDVIKGVPCIKRYHQLFCPTAGNSYPIDKIERFIDDNKALMRRMYGDFEMNMEGPGGGGGRQQGKVRKRRFIDEPDIFIPPGAFAANAGETVEAGDSYFGQLRKKRQAAAGGSRNRGGSAGGSGNGNTNANRQPGNKNGSSGTGRLDACESKIEIVTPYWASNSAGKIRAIVNTQHFEQAIHQEVCSNTQTPRCEGECGCEQKYKWHRLLAYDPDNDCKGIFMDWFLFPSCCVCRCNP

Target ORF information:

RefSeq Version	NM_135316.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spatzle 3 (spz3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135316.3

ORF Insert Sequence:

ATGGCTCTGA CCAATTTCTC GCTGCCCTTC GGTGCCCTGG GCCAGCCCTG GGGAGTCACC 
ATAGCTCCCC TGCATCCAAT CCACCAGTTG GCCAGCAACA CAAATAATCT GCTCTACTCG 
CCAGCTGACC ACCAGCAGCA GACTCCCGCG GAGGCAGCCG CCGATCCGGA GTACTTCAAG 
AACAATCCCT ATGCGCCGCC GCAATCGGGT GGTTATCAAT ATCAAAATAC AGCTGGACGA 
CGCAAGCAGA GTAACGCCTA TTTGCCACCG ACAGCGCCGA ATGCTGTCCG GAATTCCGTT 
TACCACATCC AGCAGGTACA GCAGACGCAG CAGCAGCAAA CGCAACAACA ACACCAGCAG 
CAGGATCAGC ATGAGAACAG CGTGTCCTTC CAGAGCAGCA GTTCCAGATC AAGTTCGAGC 
AGCACCACGG GCCAAAGTTC CATCCAGCTG ACACAGACTC ATGCGAGTGG CCGGGGACCG 
GCGGAGGGTT CGTACTCCCG TTATCCCGGA CAGCAAGCAC AGCCGCCGCA GCAGCAGCAA 
CCGCAACAGA AGCAATATTT CAATGCACAC GGCAGTGCCA GTGCCACTTT TACTAAGAAC 
AGCGGCAGCT TTAGTATTAC CAGCTTTGGC AGCAGGCAGC AGCAGCAGCA GCCACCGCAG 
CCGCAACAAC CACCGCCATC GCAACAGCAG CAACCACCAC CTGCACCACC GCCCCAAAGG 
TCCAGGCAGG CCAAGCCGGA GGCTCAACCA GCTCAGACAT ACGGAGTTGC CCCGCCGGAG 
AATTATCCGG AACGAGCACC AGGATTTACA CGGGTTCAGG CTGGTCAGGG ATCCAGGACT 
CAGGTACACG CCGTTCTAGA CTACGACGTG GAGGAGGGTG AGGAGGATGA GGAGGAGGAC 
GGAGAGGAGG AAGGACAGTT CTACGAAGGC CAAGAGAATG ATAAGTCGAA TAACAATCAG 
ATGCCCACAG TGACGCCCAT CCAGGGACCG ATTTACTTGA AGAACGGGAC GGTTCCAGTG 
GTGCCGCTCT TCTCCTATCC GAAACTCAAC AACGGATCGT TCCTACAGAT TCCGATCTGG 
TGGACGGCTT TGTCGGTGGC TTTGGGACTG GATGTGCGCG GCGATGTCAT CAAGGGAGTG 
CCGTGCATCA AGCGATATCA TCAACTGTTT TGCCCCACGG CCGGCAATAG TTATCCCATT 
GACAAGATTG AACGTTTCAT AGACGACAAC AAGGCCCTGA TGCGACGGAT GTACGGCGAC 
TTTGAGATGA ACATGGAGGG TCCTGGCGGA GGAGGTGGCA GACAACAAGG CAAGGTGCGA 
AAGCGTCGCT TCATAGACGA GCCGGATATA TTCATACCCC CGGGAGCATT TGCTGCCAAT 
GCCGGGGAGA CCGTCGAAGC CGGAGATAGT TACTTTGGCC AACTCCGGAA AAAGCGCCAA 
GCTGCCGCCG GAGGAAGTCG AAATAGAGGC GGATCTGCCG GAGGAAGTGG CAATGGGAAC 
ACGAATGCCA ATAGGCAACC GGGAAATAAG AATGGTAGTT CGGGCACTGG AAGACTCGAT 
GCCTGCGAGT CGAAGATTGA AATTGTCACA CCGTACTGGG CATCGAACTC CGCGGGTAAG 
ATCAGAGCCA TCGTAAATAC TCAGCATTTC GAGCAGGCGA TTCACCAGGA GGTTTGCAGC 
AATACCCAAA CTCCAAGATG TGAGGGCGAG TGTGGATGCG AGCAGAAATA CAAATGGCAT 
AGATTACTCG CCTACGATCC TGATAACGAT TGCAAGGGCA TTTTTATGGA TTGGTTCCTG 
TTTCCTTCGT GCTGTGTCTG TAGATGTAAT CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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spz3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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