Gart cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gart
Entrez Gene ID	33986
Full Name	adenosine 3
Synonyms	143062_at,ADE8,Ade3,CG31628,CG8761,D,Dmel\CG31628,GART,Gart,Gart/ade3,gart,l(2)27Da
General protein information	Preferred Names adenosine 3 Names CG31628 gene product from transcript CG31628-RC CG31628-PA CG31628-PB CG31628-PC ade3-PA ade3-PB ade3-PC complementation group D
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 27D4-27D4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001014477.2	NP_001014477.1	GART trifunctional enzyme, isoform B
NM_078773.3	NP_523497.2	GART trifunctional enzyme, isoform A
NM_001298769.1	NP_001285698.1	GART trifunctional enzyme, isoform C

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GART	XP_003319102.1
Canis lupus familiaris (dog)	LOC487740	XP_544864.3
Gallus gallus (chicken)	GART	NP_001001469.1
Caenorhabditis elegans (roundworm)	F38B6.4	NP_509122.2
Kluyveromyces lactis	KLLA0A00957g	XP_451041.1
Schizosaccharomyces pombe (fission yeast)	ade1	NP_596304.1
Homo sapiens (human)	GART	NP_000810.1
Canis lupus familiaris (dog)	LOC100856570	XP_005638901.1
Bos taurus (cattle)	GART	NP_001035563.1
Mus musculus (house mouse)	Gart	NP_034386.2
Drosophila melanogaster (fruit fly)	ade3	NP_523497.2
Neurospora crassa	NCU00177	XP_956400.1
Oryza sativa (rice)	Os08g0191200	NP_001061170.1
Eremothecium gossypii	AGOS_AFR254C	NP_985801.2
Magnaporthe oryzae (rice blast fungus)	MGG_11343	XP_003713782.1
	GART	XP_001093303.2
Danio rerio (zebrafish)	gart	NP_571692.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009786	XP_318881.4
Arabidopsis thaliana (thale cress)	AT1G09830	NP_172454.1
Oryza sativa (rice)	Os12g0197100	NP_001066357.1
Xenopus tropicalis (tropical clawed frog)	gart	NP_001011441.1
Rattus norvegicus (Norway rat)	Gart	NP_001011899.2
Saccharomyces cerevisiae (baker's yeast)	ADE5,7	NP_011280.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Gart gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gart cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa13991	NM_001014477.2 Latest version!	Drosophila melanogaster GART trifunctional enzyme (Gart), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa13990	NM_001298769.1 Latest version!	Drosophila melanogaster GART trifunctional enzyme (Gart), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa13990	NM_078773.3 Latest version!	Drosophila melanogaster GART trifunctional enzyme (Gart), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gart ( NM_001014477.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001014477.1 Drosophila melanogaster GART trifunctional enzyme (Gart), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa13991
Clone ID Related Accession (Same CDS sequence)	NM_001014477.2
Accession Version	NM_001014477.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	GART trifunctional enzyme, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001014477.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCATC GCGTTCTGGT CATTGGCAGC GGCGGTCGGG AGCACGCGAT CTGCTGGAAG 
TTGTCGCAAT CTCCGAAGGT GGCCCAAATA TACGCACTGC CGGGTAGCCA TGGCATCCAG 
CTGGTGGAGA AGTGCCGGAA TCTGGATGCC AAGACCTTGG ATCCTAAGGA TTTCGAGGCC 
ATTGCTAAAT GGAGCAAGGA GAACCAGATA GCCCTAGTGG TTGTGGGACC CGAAGATCCC 
TTGGCTTTGG GTTTGGGCGA TGTGCTGCAG AGCGCTGGAA TTCCCTGCTT TGGACCCGGC 
AAGCAAGGTG CCCAAATCGA GGCGGACAAG AAGTGGGCCA AGGACTTTAT GCTGCGCCAT 
GGGATTCCCA CAGCGCGTTA CGAAAGTTTC ACAGACACCG AAAAGGCCAA GGCATTCATA 
AGAAGCGCTC CGTATCCAGC TCTGGTGGTG AAAGCTGCTG GATTGGCCGC CGGAAAGGGC 
GTGGTGGTGG CCGCCAATGC GAAGGAGGCC TGCCAGGCGG TGGACGAGAT CCTGGGAGAT 
CTGAAATACG GTCAGGCTGG CGCCACGCTG GTGGTGGAGG AGCTACTGGA GGGCGAAGAG 
GTTTCCGTTC TGGCCTTCAC CGATGGCAAA AGTGTGCGTG CCATGCTGCC AGCGCAGGAT 
CACAAGCGAC TGGGCAATGG CGATACTGGA CCAAACACCG GCGGCATGGG CGCCTACTGC 
CCTTGTCCGT TGATCAGCCA GCCAGCACTG GAACTGGTGC AAAAGGCCGT GCTGGAGCGG 
GCTGTGCAGG GTCTGATCAA GGAGAGGATC AACTACCAGG GTGTACTCTA TGCGGGACTC 
ATGCTGACAC GCGATGGTCC CCGTGTGCTG GAGTTTAACT GCCGGTTTGG AGATCCCGAG 
ACCCAGGTGA TTCTGCCGCT GCTCGAAAGC GATCTGTTCG ATGTGATGGA GGCTTGTTGC 
AGCGGAAAGC TGGACAAGAT TCCCCTGCAA TGGCGCAATG GAGTGAGTGC CGTTGGTGTG 
ATCCTGGCCA GTGCTGGCTA TCCGGAGACC TCCACAAAGG GTTGCATCAT ATCGGGACTT 
CCGGCTGCCA ATACACCCAC CCAACTGGTC TTTCACAGCG GCCTTGCCGT GAATGCACAA 
AAGGAAGCCC TAACCAATGG CGGTCGCGTC CTCATCGCGA TCGCTCTGGA CGGCAGCTTG 
AAGGAGGCTG CTGCGAAGGC AACCAAATTG GCTGGCAGCA TCAGTTTTTC GGGCTCTGGA 
GCTCAGTACC GAACGGACAT TGCCCAGAAG GCGTTCAAAA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014477.1
CDS	194..1498
Translation	MSHRVLVIGSGGREHAICWKLSQSPKVAQIYALPGSHGIQLVEKCRNLDAKTLDPKDFEAIAKWSKENQIALVVVGPEDPLALGLGDVLQSAGIPCFGPGKQGAQIEADKKWAKDFMLRHGIPTARYESFTDTEKAKAFIRSAPYPALVVKAAGLAAGKGVVVAANAKEACQAVDEILGDLKYGQAGATLVVEELLEGEEVSVLAFTDGKSVRAMLPAQDHKRLGNGDTGPNTGGMGAYCPCPLISQPALELVQKAVLERAVQGLIKERINYQGVLYAGLMLTRDGPRVLEFNCRFGDPETQVILPLLESDLFDVMEACCSGKLDKIPLQWRNGVSAVGVILASAGYPETSTKGCIISGLPAANTPTQLVFHSGLAVNAQKEALTNGGRVLIAIALDGSLKEAAAKATKLAGSISFSGSGAQYRTDIAQKAFKM

Target ORF information:

RefSeq Version	NM_001014477.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster GART trifunctional enzyme (Gart), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014477.2

ORF Insert Sequence:

ATGTCGCATC GCGTTCTGGT CATTGGCAGC GGCGGTCGGG AGCACGCGAT CTGCTGGAAG 
TTGTCGCAAT CTCCGAAGGT GGCCCAAATA TACGCACTGC CGGGTAGCCA TGGCATCCAG 
CTGGTGGAGA AGTGCCGGAA TCTGGATGCC AAGACCTTGG ATCCTAAGGA TTTCGAGGCC 
ATTGCTAAAT GGAGCAAGGA GAACCAGATA GCCCTAGTGG TTGTGGGACC CGAAGATCCC 
TTGGCTTTGG GTTTGGGCGA TGTGCTGCAG AGCGCTGGAA TTCCCTGCTT TGGACCCGGC 
AAGCAAGGTG CCCAAATCGA GGCGGACAAG AAGTGGGCCA AGGACTTTAT GCTGCGCCAT 
GGGATTCCCA CAGCGCGTTA CGAAAGTTTC ACAGACACCG AAAAGGCCAA GGCATTCATA 
AGAAGCGCTC CGTATCCAGC TCTGGTGGTG AAAGCTGCTG GATTGGCCGC CGGAAAGGGC 
GTGGTGGTGG CCGCCAATGC GAAGGAGGCC TGCCAGGCGG TGGACGAGAT CCTGGGAGAT 
CTGAAATACG GTCAGGCTGG CGCCACGCTG GTGGTGGAGG AGCTACTGGA GGGCGAAGAG 
GTTTCCGTTC TGGCCTTCAC CGATGGCAAA AGTGTGCGTG CCATGCTGCC AGCGCAGGAT 
CACAAGCGAC TGGGCAATGG CGATACTGGA CCAAACACCG GCGGCATGGG CGCCTACTGC 
CCTTGTCCGT TGATCAGCCA GCCAGCACTG GAACTGGTGC AAAAGGCCGT GCTGGAGCGG 
GCTGTGCAGG GTCTGATCAA GGAGAGGATC AACTACCAGG GTGTACTCTA TGCGGGACTC 
ATGCTGACAC GCGATGGTCC CCGTGTGCTG GAGTTTAACT GCCGGTTTGG AGATCCCGAG 
ACCCAGGTGA TTCTGCCGCT GCTCGAAAGC GATCTGTTCG ATGTGATGGA GGCTTGTTGC 
AGCGGAAAGC TGGACAAGAT TCCCCTGCAA TGGCGCAATG GAGTGAGTGC CGTTGGTGTG 
ATCCTGGCCA GTGCTGGCTA TCCGGAGACC TCCACAAAGG GTTGCATCAT ATCGGGACTT 
CCGGCTGCCA ATACACCCAC CCAACTGGTC TTTCACAGCG GCCTTGCCGT GAATGCACAA 
AAGGAAGCCC TAACCAATGG CGGTCGCGTC CTCATCGCGA TCGCTCTGGA CGGCAGCTTG 
AAGGAGGCTG CTGCGAAGGC AACCAAATTG GCTGGCAGCA TCAGTTTTTC GGGCTCTGGA 
GCTCAGTACC GAACGGACAT TGCCCAGAAG GCGTTCAAAA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gart ( NM_078773.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523497.2 Drosophila melanogaster GART trifunctional enzyme (Gart), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa13990
Clone ID Related Accession (Same CDS sequence)	NM_078773.3 , NM_001298769.1
Accession Version	NM_078773.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	GART trifunctional enzyme, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_078773.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCATC GCGTTCTGGT CATTGGCAGC GGCGGTCGGG AGCACGCGAT CTGCTGGAAG 
TTGTCGCAAT CTCCGAAGGT GGCCCAAATA TACGCACTGC CGGGTAGCCA TGGCATCCAG 
CTGGTGGAGA AGTGCCGGAA TCTGGATGCC AAGACCTTGG ATCCTAAGGA TTTCGAGGCC 
ATTGCTAAAT GGAGCAAGGA GAACCAGATA GCCCTAGTGG TTGTGGGACC CGAAGATCCC 
TTGGCTTTGG GTTTGGGCGA TGTGCTGCAG AGCGCTGGAA TTCCCTGCTT TGGACCCGGC 
AAGCAAGGTG CCCAAATCGA GGCGGACAAG AAGTGGGCCA AGGACTTTAT GCTGCGCCAT 
GGGATTCCCA CAGCGCGTTA CGAAAGTTTC ACAGACACCG AAAAGGCCAA GGCATTCATA 
AGAAGCGCTC CGTATCCAGC TCTGGTGGTG AAAGCTGCTG GATTGGCCGC CGGAAAGGGC 
GTGGTGGTGG CCGCCAATGC GAAGGAGGCC TGCCAGGCGG TGGACGAGAT CCTGGGAGAT 
CTGAAATACG GTCAGGCTGG CGCCACGCTG GTGGTGGAGG AGCTACTGGA GGGCGAAGAG 
GTTTCCGTTC TGGCCTTCAC CGATGGCAAA AGTGTGCGTG CCATGCTGCC AGCGCAGGAT 
CACAAGCGAC TGGGCAATGG CGATACTGGA CCAAACACCG GCGGCATGGG CGCCTACTGC 
CCTTGTCCGT TGATCAGCCA GCCAGCACTG GAACTGGTGC AAAAGGCCGT GCTGGAGCGG 
GCTGTGCAGG GTCTGATCAA GGAGAGGATC AACTACCAGG GTGTACTCTA TGCGGGACTC 
ATGCTGACAC GCGATGGTCC CCGTGTGCTG GAGTTTAACT GCCGGTTTGG AGATCCCGAG 
ACCCAGGTGA TTCTGCCGCT GCTCGAAAGC GATCTGTTCG ATGTGATGGA GGCTTGTTGC 
AGCGGAAAGC TGGACAAGAT TCCCCTGCAA TGGCGCAATG GAGTGAGTGC CGTTGGTGTG 
ATCCTGGCCA GTGCTGGCTA TCCGGAGACC TCCACAAAGG GTTGCATCAT ATCGGGACTT 
CCGGCTGCCA ATACACCCAC CCAACTGGTC TTTCACAGCG GCCTTGCCGT GAATGCACAA 
AAGGAAGCCC TAACCAATGG CGGTCGCGTC CTCATCGCGA TCGCTCTGGA CGGCAGCTTG 
AAGGAGGCTG CTGCGAAGGC AACCAAATTG GCTGGCAGCA TCAGTTTTTC GGGCTCTGGA 
GCTCAGTACC GAACGGACAT TGCCCAGAAG GCGTTCAAAA TTGCCAGTGC CAGCACCCCG 
GGGTTGAGTT ACAAGGACAG CGGAGTGGAC ATCGATGCTG GAGATGCACT GGTGCAACGC 
ATCAAGCCCT TGTCTCGGGG TACCCAGCGC CCGGGCGTGA TTGGCGGCTT GGGTGGATTT 
GGAGGACTGT TCCGTTTGAA GGAACTCACC TACAAGGAGC CAGTCATTGC TGAGGCCACT 
CAGGGAGTCG GAGCCAAGAT CCATCTTGCC CTGACGCACG AATTGTATGA AAATGTGGGC 
TACGACCTGT TTGCCTTGGC TGCTAATGAT GTCCTGGAAG TGGGTGCCGA GCCGGTCGCC 
TTTCTGGACT ACATTGCCTG TGGCAAGCTG CAAGTTCCAT TGGCCGCCCA GTTGGTCAAG 
GGAATGGCTG ATGGCTGCCG GGATGCGCGG TGCGCCTTAG TGGGTGGTGA GACTGCAGAG 
ATGCCTTCTC TATATGCTCC CGGTCAACAC GACATGGCTG GCTATTGTGT TGGCATAGTG 
GAGCAGTCTC GAATCCTGCC CCGTTTCGAT CTTTACCAGC CGGGTGACTT ACTCATCGGT 
CTGCCATCAT CTGGGCTTCA TTGCGCCGGC TTCAATGAAA TACTCACCCA GTTGGCGGCA 
TCCAAAGTGA ATCTCAGAGA GCGTTCTCCC GTGGACGGAG GCGACGATGG GCTGACCTTG 
GCCCATGTCT TAGCCACGCC CACCCAACTG TATGTCCAGC AGCTGTTGCC TCATCTTCAG 
AAAGGAGATG AGATCAAGTC GGTGGCTCAC GTGACCCATG GTCTGCTAAA CGATATCCTC 
CGTCTGCTTC CCGATGGATT TGAGACAACG CTGGATTTCG GTGCCGTCCC AGTGCCCAAA 
ATCTTTGGTT GGCTGGCCGG AAAGCTAAAG CTGAGTGCCC AAACAATTCT AGAGCGGCAC 
AACTGCGGCA TTGGCATGGT TCTGATCCTG CCTCAGTCAA GTCAGCTGTG GCGAACGTCC 
CTTCCGGGAG CCAAAGTACT GGGCGTCCTG CAACGGCGGT CAAAGGTTAG TGGATCACCT 
GTCCAGGTGC GCAACTTTGT GGAGCAACTG GAGAAGGTGG CCTCGCCGTT CGGTGGTCTC 
GGCGATCGGG AATTGCCCGA AGAGCTCAAG AAACTGCCTT CCAACTCGGA TTTATCAGCT 
CCTCGCGAGG AATGCTTTGA AAACGCAGCT GGACGTCGGC TCACGCGCAT ACCCACCCAC 
TACAAGGACC CCATACTCAT CCTGGGCACC GATGGAGTGG GCACCAAGCT GAAAATAGCG 
CAGCAAACGA ATCGCAACAC GAGCGTGGGT ATCGATTTGG TGGCCATGTG CGTCAACGAC 
ATCCTCTGCA ATGGAGCCGA GCCAATCAGT TTCTCAAGCT ACTATGCCTG CGGGCACTGG 
CAGGAGCAGT TGGCCAAGGA GGTTCACTCC GGGGTCCAAG AGGGTGCCAG GCAGGCGAAC 
AGCAGTTTCA TTGACTCACA TAGTGCCGCT CTGCCGCTCC TGTACGAACC CCAGGTCTAT 
GATCTGGCTG GTTTCGCCTT GGGCATAGCA GAACACACCG GAATTCTACC CCTTTTGGAT 
GAAATCCAGC CCGGAGATGT GCTCATCGGC TTACCTTCGT CTGGCGTCCA CAGCAACGGA 
TTTAGTTTGG TTCACGCCGT TTTGAAGCGA GTGGGCTTGG GTCTGCATGA CAAGGCGCCC 
TTTAGTGATA AGACGCTGGG CGAGGAGCTG CTGGTGCCCA CCAAGATCTA TGTCAAGGCA 
TTGTCCACTC TGCTGTCCCG CGGAAAACAC GGCATCAAGG CACTAGCCCA CATCACAGGA 
GGAGGCCTGA GCGAGAATAT ACCGCGAGTA TTGCGTAAGG ATTTGGCCGT GCGCCTGGAT 
GCCAATAAGT TCCAGCTTCC GCCCGTTTTC GCCTGGCTTG CAGCGGCCGG AAATATCAGC 
TCCACTGAGC TGCAGCGCAC ATATAACTGC GGCTTGGGGA TGGTTCTGGT GGTGGCCCCG 
ACGGAAGTCG AGGACGTTCT TAAGGAGCTG CGTTATCCGC AAAGAGCAGC TGTGGTCGGC 
GAGGTGGTGG CACGCAAGGA TCCGAAGAAG TCGCAGGTGG TGGTCCAGAA CTTTGAGGCA 
TCATTGGCCA GGACACAGAA GATGCTCTCG CAGCGTCGGA AACGAGTTGC TGTTCTCATT 
TCGGGAACGG GCAGCAACCT GCAAGCACTA ATTGATGCAA CGCGCGACTC GGCACAGGGT 
ATCCATGCCG ATGTTGTGCT GGTAATCAGC AATAAGACTG GTGTTCTGGG TCTGCAGCGA 
GCCACGCAAG CCGGAATACC TTCGTTGGTC ATCTCCCACA AGGATTTCGC TAGCCGAGAG 
GTCTATGATG CGGAACTTAC GCGAAATCTG AAGGCGGCTC GTGTGGATTT GATCTGCCTT 
GCCGGCTTCA TGCGTGTCCT GAGTGCTCCA TTCGTTCGAG AGTGGCGTGG ACGGCTCGTC 
AATATCCATC CCTCGCTGCT GCCCAAGTAT CCTGGTCTGC ATGTCCAGAA GCAGGCCTTG 
GAGGCGGGCG AAAAGGAGTC CGGCTGCACG GTTCACTTCG TGGACGAGGG TGTGGACACG 
GGAGCGATCA TTGTTCAGGC TGCTGTTCCC ATTTTGCCCG ATGACGACGA GGATTCGCTG 
ACCCAACGCA TCCACAAGGC TGAGCACTGG GCTTTTCCAA GGGCATTAGC AATGTTGGTC 
AACGGAACAG CTCTCATTTC TCCGGAAGTC AGCAGTCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523497.2
CDS	194..4255
Translation	MSHRVLVIGSGGREHAICWKLSQSPKVAQIYALPGSHGIQLVEKCRNLDAKTLDPKDFEAIAKWSKENQIALVVVGPEDPLALGLGDVLQSAGIPCFGPGKQGAQIEADKKWAKDFMLRHGIPTARYESFTDTEKAKAFIRSAPYPALVVKAAGLAAGKGVVVAANAKEACQAVDEILGDLKYGQAGATLVVEELLEGEEVSVLAFTDGKSVRAMLPAQDHKRLGNGDTGPNTGGMGAYCPCPLISQPALELVQKAVLERAVQGLIKERINYQGVLYAGLMLTRDGPRVLEFNCRFGDPETQVILPLLESDLFDVMEACCSGKLDKIPLQWRNGVSAVGVILASAGYPETSTKGCIISGLPAANTPTQLVFHSGLAVNAQKEALTNGGRVLIAIALDGSLKEAAAKATKLAGSISFSGSGAQYRTDIAQKAFKIASASTPGLSYKDSGVDIDAGDALVQRIKPLSRGTQRPGVIGGLGGFGGLFRLKELTYKEPVIAEATQGVGAKIHLALTHELYENVGYDLFALAANDVLEVGAEPVAFLDYIACGKLQVPLAAQLVKGMADGCRDARCALVGGETAEMPSLYAPGQHDMAGYCVGIVEQSRILPRFDLYQPGDLLIGLPSSGLHCAGFNEILTQLAASKVNLRERSPVDGGDDGLTLAHVLATPTQLYVQQLLPHLQKGDEIKSVAHVTHGLLNDILRLLPDGFETTLDFGAVPVPKIFGWLAGKLKLSAQTILERHNCGIGMVLILPQSSQLWRTSLPGAKVLGVLQRRSKVSGSPVQVRNFVEQLEKVASPFGGLGDRELPEELKKLPSNSDLSAPREECFENAAGRRLTRIPTHYKDPILILGTDGVGTKLKIAQQTNRNTSVGIDLVAMCVNDILCNGAEPISFSSYYACGHWQEQLAKEVHSGVQEGARQANSSFIDSHSAALPLLYEPQVYDLAGFALGIAEHTGILPLLDEIQPGDVLIGLPSSGVHSNGFSLVHAVLKRVGLGLHDKAPFSDKTLGEELLVPTKIYVKALSTLLSRGKHGIKALAHITGGGLSENIPRVLRKDLAVRLDANKFQLPPVFAWLAAAGNISSTELQRTYNCGLGMVLVVAPTEVEDVLKELRYPQRAAVVGEVVARKDPKKSQVVVQNFEASLARTQKMLSQRRKRVAVLISGTGSNLQALIDATRDSAQGIHADVVLVISNKTGVLGLQRATQAGIPSLVISHKDFASREVYDAELTRNLKAARVDLICLAGFMRVLSAPFVREWRGRLVNIHPSLLPKYPGLHVQKQALEAGEKESGCTVHFVDEGVDTGAIIVQAAVPILPDDDEDSLTQRIHKAEHWAFPRALAMLVNGTALISPEVSSQ

Target ORF information:

RefSeq Version	NM_078773.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster GART trifunctional enzyme (Gart), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078773.3

ORF Insert Sequence:

ATGTCGCATC GCGTTCTGGT CATTGGCAGC GGCGGTCGGG AGCACGCGAT CTGCTGGAAG 
TTGTCGCAAT CTCCGAAGGT GGCCCAAATA TACGCACTGC CGGGTAGCCA TGGCATCCAG 
CTGGTGGAGA AGTGCCGGAA TCTGGATGCC AAGACCTTGG ATCCTAAGGA TTTCGAGGCC 
ATTGCTAAAT GGAGCAAGGA GAACCAGATA GCCCTAGTGG TTGTGGGACC CGAAGATCCC 
TTGGCTTTGG GTTTGGGCGA TGTGCTGCAG AGCGCTGGAA TTCCCTGCTT TGGACCCGGC 
AAGCAAGGTG CCCAAATCGA GGCGGACAAG AAGTGGGCCA AGGACTTTAT GCTGCGCCAT 
GGGATTCCCA CAGCGCGTTA CGAAAGTTTC ACAGACACCG AAAAGGCCAA GGCATTCATA 
AGAAGCGCTC CGTATCCAGC TCTGGTGGTG AAAGCTGCTG GATTGGCCGC CGGAAAGGGC 
GTGGTGGTGG CCGCCAATGC GAAGGAGGCC TGCCAGGCGG TGGACGAGAT CCTGGGAGAT 
CTGAAATACG GTCAGGCTGG CGCCACGCTG GTGGTGGAGG AGCTACTGGA GGGCGAAGAG 
GTTTCCGTTC TGGCCTTCAC CGATGGCAAA AGTGTGCGTG CCATGCTGCC AGCGCAGGAT 
CACAAGCGAC TGGGCAATGG CGATACTGGA CCAAACACCG GCGGCATGGG CGCCTACTGC 
CCTTGTCCGT TGATCAGCCA GCCAGCACTG GAACTGGTGC AAAAGGCCGT GCTGGAGCGG 
GCTGTGCAGG GTCTGATCAA GGAGAGGATC AACTACCAGG GTGTACTCTA TGCGGGACTC 
ATGCTGACAC GCGATGGTCC CCGTGTGCTG GAGTTTAACT GCCGGTTTGG AGATCCCGAG 
ACCCAGGTGA TTCTGCCGCT GCTCGAAAGC GATCTGTTCG ATGTGATGGA GGCTTGTTGC 
AGCGGAAAGC TGGACAAGAT TCCCCTGCAA TGGCGCAATG GAGTGAGTGC CGTTGGTGTG 
ATCCTGGCCA GTGCTGGCTA TCCGGAGACC TCCACAAAGG GTTGCATCAT ATCGGGACTT 
CCGGCTGCCA ATACACCCAC CCAACTGGTC TTTCACAGCG GCCTTGCCGT GAATGCACAA 
AAGGAAGCCC TAACCAATGG CGGTCGCGTC CTCATCGCGA TCGCTCTGGA CGGCAGCTTG 
AAGGAGGCTG CTGCGAAGGC AACCAAATTG GCTGGCAGCA TCAGTTTTTC GGGCTCTGGA 
GCTCAGTACC GAACGGACAT TGCCCAGAAG GCGTTCAAAA TTGCCAGTGC CAGCACCCCG 
GGGTTGAGTT ACAAGGACAG CGGAGTGGAC ATCGATGCTG GAGATGCACT GGTGCAACGC 
ATCAAGCCCT TGTCTCGGGG TACCCAGCGC CCGGGCGTGA TTGGCGGCTT GGGTGGATTT 
GGAGGACTGT TCCGTTTGAA GGAACTCACC TACAAGGAGC CAGTCATTGC TGAGGCCACT 
CAGGGAGTCG GAGCCAAGAT CCATCTTGCC CTGACGCACG AATTGTATGA AAATGTGGGC 
TACGACCTGT TTGCCTTGGC TGCTAATGAT GTCCTGGAAG TGGGTGCCGA GCCGGTCGCC 
TTTCTGGACT ACATTGCCTG TGGCAAGCTG CAAGTTCCAT TGGCCGCCCA GTTGGTCAAG 
GGAATGGCTG ATGGCTGCCG GGATGCGCGG TGCGCCTTAG TGGGTGGTGA GACTGCAGAG 
ATGCCTTCTC TATATGCTCC CGGTCAACAC GACATGGCTG GCTATTGTGT TGGCATAGTG 
GAGCAGTCTC GAATCCTGCC CCGTTTCGAT CTTTACCAGC CGGGTGACTT ACTCATCGGT 
CTGCCATCAT CTGGGCTTCA TTGCGCCGGC TTCAATGAAA TACTCACCCA GTTGGCGGCA 
TCCAAAGTGA ATCTCAGAGA GCGTTCTCCC GTGGACGGAG GCGACGATGG GCTGACCTTG 
GCCCATGTCT TAGCCACGCC CACCCAACTG TATGTCCAGC AGCTGTTGCC TCATCTTCAG 
AAAGGAGATG AGATCAAGTC GGTGGCTCAC GTGACCCATG GTCTGCTAAA CGATATCCTC 
CGTCTGCTTC CCGATGGATT TGAGACAACG CTGGATTTCG GTGCCGTCCC AGTGCCCAAA 
ATCTTTGGTT GGCTGGCCGG AAAGCTAAAG CTGAGTGCCC AAACAATTCT AGAGCGGCAC 
AACTGCGGCA TTGGCATGGT TCTGATCCTG CCTCAGTCAA GTCAGCTGTG GCGAACGTCC 
CTTCCGGGAG CCAAAGTACT GGGCGTCCTG CAACGGCGGT CAAAGGTTAG TGGATCACCT 
GTCCAGGTGC GCAACTTTGT GGAGCAACTG GAGAAGGTGG CCTCGCCGTT CGGTGGTCTC 
GGCGATCGGG AATTGCCCGA AGAGCTCAAG AAACTGCCTT CCAACTCGGA TTTATCAGCT 
CCTCGCGAGG AATGCTTTGA AAACGCAGCT GGACGTCGGC TCACGCGCAT ACCCACCCAC 
TACAAGGACC CCATACTCAT CCTGGGCACC GATGGAGTGG GCACCAAGCT GAAAATAGCG 
CAGCAAACGA ATCGCAACAC GAGCGTGGGT ATCGATTTGG TGGCCATGTG CGTCAACGAC 
ATCCTCTGCA ATGGAGCCGA GCCAATCAGT TTCTCAAGCT ACTATGCCTG CGGGCACTGG 
CAGGAGCAGT TGGCCAAGGA GGTTCACTCC GGGGTCCAAG AGGGTGCCAG GCAGGCGAAC 
AGCAGTTTCA TTGACTCACA TAGTGCCGCT CTGCCGCTCC TGTACGAACC CCAGGTCTAT 
GATCTGGCTG GTTTCGCCTT GGGCATAGCA GAACACACCG GAATTCTACC CCTTTTGGAT 
GAAATCCAGC CCGGAGATGT GCTCATCGGC TTACCTTCGT CTGGCGTCCA CAGCAACGGA 
TTTAGTTTGG TTCACGCCGT TTTGAAGCGA GTGGGCTTGG GTCTGCATGA CAAGGCGCCC 
TTTAGTGATA AGACGCTGGG CGAGGAGCTG CTGGTGCCCA CCAAGATCTA TGTCAAGGCA 
TTGTCCACTC TGCTGTCCCG CGGAAAACAC GGCATCAAGG CACTAGCCCA CATCACAGGA 
GGAGGCCTGA GCGAGAATAT ACCGCGAGTA TTGCGTAAGG ATTTGGCCGT GCGCCTGGAT 
GCCAATAAGT TCCAGCTTCC GCCCGTTTTC GCCTGGCTTG CAGCGGCCGG AAATATCAGC 
TCCACTGAGC TGCAGCGCAC ATATAACTGC GGCTTGGGGA TGGTTCTGGT GGTGGCCCCG 
ACGGAAGTCG AGGACGTTCT TAAGGAGCTG CGTTATCCGC AAAGAGCAGC TGTGGTCGGC 
GAGGTGGTGG CACGCAAGGA TCCGAAGAAG TCGCAGGTGG TGGTCCAGAA CTTTGAGGCA 
TCATTGGCCA GGACACAGAA GATGCTCTCG CAGCGTCGGA AACGAGTTGC TGTTCTCATT 
TCGGGAACGG GCAGCAACCT GCAAGCACTA ATTGATGCAA CGCGCGACTC GGCACAGGGT 
ATCCATGCCG ATGTTGTGCT GGTAATCAGC AATAAGACTG GTGTTCTGGG TCTGCAGCGA 
GCCACGCAAG CCGGAATACC TTCGTTGGTC ATCTCCCACA AGGATTTCGC TAGCCGAGAG 
GTCTATGATG CGGAACTTAC GCGAAATCTG AAGGCGGCTC GTGTGGATTT GATCTGCCTT 
GCCGGCTTCA TGCGTGTCCT GAGTGCTCCA TTCGTTCGAG AGTGGCGTGG ACGGCTCGTC 
AATATCCATC CCTCGCTGCT GCCCAAGTAT CCTGGTCTGC ATGTCCAGAA GCAGGCCTTG 
GAGGCGGGCG AAAAGGAGTC CGGCTGCACG GTTCACTTCG TGGACGAGGG TGTGGACACG 
GGAGCGATCA TTGTTCAGGC TGCTGTTCCC ATTTTGCCCG ATGACGACGA GGATTCGCTG 
ACCCAACGCA TCCACAAGGC TGAGCACTGG GCTTTTCCAA GGGCATTAGC AATGTTGGTC 
AACGGAACAG CTCTCATTTC TCCGGAAGTC AGCAGTCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gart ( NM_001298769.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285698.1 Drosophila melanogaster GART trifunctional enzyme (Gart), transcript variant C, mRNA.

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CloneID	OFa13990
Clone ID Related Accession (Same CDS sequence)	NM_078773.3 , NM_001298769.1
Accession Version	NM_001298769.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	GART trifunctional enzyme, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCATC GCGTTCTGGT CATTGGCAGC GGCGGTCGGG AGCACGCGAT CTGCTGGAAG 
TTGTCGCAAT CTCCGAAGGT GGCCCAAATA TACGCACTGC CGGGTAGCCA TGGCATCCAG 
CTGGTGGAGA AGTGCCGGAA TCTGGATGCC AAGACCTTGG ATCCTAAGGA TTTCGAGGCC 
ATTGCTAAAT GGAGCAAGGA GAACCAGATA GCCCTAGTGG TTGTGGGACC CGAAGATCCC 
TTGGCTTTGG GTTTGGGCGA TGTGCTGCAG AGCGCTGGAA TTCCCTGCTT TGGACCCGGC 
AAGCAAGGTG CCCAAATCGA GGCGGACAAG AAGTGGGCCA AGGACTTTAT GCTGCGCCAT 
GGGATTCCCA CAGCGCGTTA CGAAAGTTTC ACAGACACCG AAAAGGCCAA GGCATTCATA 
AGAAGCGCTC CGTATCCAGC TCTGGTGGTG AAAGCTGCTG GATTGGCCGC CGGAAAGGGC 
GTGGTGGTGG CCGCCAATGC GAAGGAGGCC TGCCAGGCGG TGGACGAGAT CCTGGGAGAT 
CTGAAATACG GTCAGGCTGG CGCCACGCTG GTGGTGGAGG AGCTACTGGA GGGCGAAGAG 
GTTTCCGTTC TGGCCTTCAC CGATGGCAAA AGTGTGCGTG CCATGCTGCC AGCGCAGGAT 
CACAAGCGAC TGGGCAATGG CGATACTGGA CCAAACACCG GCGGCATGGG CGCCTACTGC 
CCTTGTCCGT TGATCAGCCA GCCAGCACTG GAACTGGTGC AAAAGGCCGT GCTGGAGCGG 
GCTGTGCAGG GTCTGATCAA GGAGAGGATC AACTACCAGG GTGTACTCTA TGCGGGACTC 
ATGCTGACAC GCGATGGTCC CCGTGTGCTG GAGTTTAACT GCCGGTTTGG AGATCCCGAG 
ACCCAGGTGA TTCTGCCGCT GCTCGAAAGC GATCTGTTCG ATGTGATGGA GGCTTGTTGC 
AGCGGAAAGC TGGACAAGAT TCCCCTGCAA TGGCGCAATG GAGTGAGTGC CGTTGGTGTG 
ATCCTGGCCA GTGCTGGCTA TCCGGAGACC TCCACAAAGG GTTGCATCAT ATCGGGACTT 
CCGGCTGCCA ATACACCCAC CCAACTGGTC TTTCACAGCG GCCTTGCCGT GAATGCACAA 
AAGGAAGCCC TAACCAATGG CGGTCGCGTC CTCATCGCGA TCGCTCTGGA CGGCAGCTTG 
AAGGAGGCTG CTGCGAAGGC AACCAAATTG GCTGGCAGCA TCAGTTTTTC GGGCTCTGGA 
GCTCAGTACC GAACGGACAT TGCCCAGAAG GCGTTCAAAA TTGCCAGTGC CAGCACCCCG 
GGGTTGAGTT ACAAGGACAG CGGAGTGGAC ATCGATGCTG GAGATGCACT GGTGCAACGC 
ATCAAGCCCT TGTCTCGGGG TACCCAGCGC CCGGGCGTGA TTGGCGGCTT GGGTGGATTT 
GGAGGACTGT TCCGTTTGAA GGAACTCACC TACAAGGAGC CAGTCATTGC TGAGGCCACT 
CAGGGAGTCG GAGCCAAGAT CCATCTTGCC CTGACGCACG AATTGTATGA AAATGTGGGC 
TACGACCTGT TTGCCTTGGC TGCTAATGAT GTCCTGGAAG TGGGTGCCGA GCCGGTCGCC 
TTTCTGGACT ACATTGCCTG TGGCAAGCTG CAAGTTCCAT TGGCCGCCCA GTTGGTCAAG 
GGAATGGCTG ATGGCTGCCG GGATGCGCGG TGCGCCTTAG TGGGTGGTGA GACTGCAGAG 
ATGCCTTCTC TATATGCTCC CGGTCAACAC GACATGGCTG GCTATTGTGT TGGCATAGTG 
GAGCAGTCTC GAATCCTGCC CCGTTTCGAT CTTTACCAGC CGGGTGACTT ACTCATCGGT 
CTGCCATCAT CTGGGCTTCA TTGCGCCGGC TTCAATGAAA TACTCACCCA GTTGGCGGCA 
TCCAAAGTGA ATCTCAGAGA GCGTTCTCCC GTGGACGGAG GCGACGATGG GCTGACCTTG 
GCCCATGTCT TAGCCACGCC CACCCAACTG TATGTCCAGC AGCTGTTGCC TCATCTTCAG 
AAAGGAGATG AGATCAAGTC GGTGGCTCAC GTGACCCATG GTCTGCTAAA CGATATCCTC 
CGTCTGCTTC CCGATGGATT TGAGACAACG CTGGATTTCG GTGCCGTCCC AGTGCCCAAA 
ATCTTTGGTT GGCTGGCCGG AAAGCTAAAG CTGAGTGCCC AAACAATTCT AGAGCGGCAC 
AACTGCGGCA TTGGCATGGT TCTGATCCTG CCTCAGTCAA GTCAGCTGTG GCGAACGTCC 
CTTCCGGGAG CCAAAGTACT GGGCGTCCTG CAACGGCGGT CAAAGGTTAG TGGATCACCT 
GTCCAGGTGC GCAACTTTGT GGAGCAACTG GAGAAGGTGG CCTCGCCGTT CGGTGGTCTC 
GGCGATCGGG AATTGCCCGA AGAGCTCAAG AAACTGCCTT CCAACTCGGA TTTATCAGCT 
CCTCGCGAGG AATGCTTTGA AAACGCAGCT GGACGTCGGC TCACGCGCAT ACCCACCCAC 
TACAAGGACC CCATACTCAT CCTGGGCACC GATGGAGTGG GCACCAAGCT GAAAATAGCG 
CAGCAAACGA ATCGCAACAC GAGCGTGGGT ATCGATTTGG TGGCCATGTG CGTCAACGAC 
ATCCTCTGCA ATGGAGCCGA GCCAATCAGT TTCTCAAGCT ACTATGCCTG CGGGCACTGG 
CAGGAGCAGT TGGCCAAGGA GGTTCACTCC GGGGTCCAAG AGGGTGCCAG GCAGGCGAAC 
AGCAGTTTCA TTGACTCACA TAGTGCCGCT CTGCCGCTCC TGTACGAACC CCAGGTCTAT 
GATCTGGCTG GTTTCGCCTT GGGCATAGCA GAACACACCG GAATTCTACC CCTTTTGGAT 
GAAATCCAGC CCGGAGATGT GCTCATCGGC TTACCTTCGT CTGGCGTCCA CAGCAACGGA 
TTTAGTTTGG TTCACGCCGT TTTGAAGCGA GTGGGCTTGG GTCTGCATGA CAAGGCGCCC 
TTTAGTGATA AGACGCTGGG CGAGGAGCTG CTGGTGCCCA CCAAGATCTA TGTCAAGGCA 
TTGTCCACTC TGCTGTCCCG CGGAAAACAC GGCATCAAGG CACTAGCCCA CATCACAGGA 
GGAGGCCTGA GCGAGAATAT ACCGCGAGTA TTGCGTAAGG ATTTGGCCGT GCGCCTGGAT 
GCCAATAAGT TCCAGCTTCC GCCCGTTTTC GCCTGGCTTG CAGCGGCCGG AAATATCAGC 
TCCACTGAGC TGCAGCGCAC ATATAACTGC GGCTTGGGGA TGGTTCTGGT GGTGGCCCCG 
ACGGAAGTCG AGGACGTTCT TAAGGAGCTG CGTTATCCGC AAAGAGCAGC TGTGGTCGGC 
GAGGTGGTGG CACGCAAGGA TCCGAAGAAG TCGCAGGTGG TGGTCCAGAA CTTTGAGGCA 
TCATTGGCCA GGACACAGAA GATGCTCTCG CAGCGTCGGA AACGAGTTGC TGTTCTCATT 
TCGGGAACGG GCAGCAACCT GCAAGCACTA ATTGATGCAA CGCGCGACTC GGCACAGGGT 
ATCCATGCCG ATGTTGTGCT GGTAATCAGC AATAAGACTG GTGTTCTGGG TCTGCAGCGA 
GCCACGCAAG CCGGAATACC TTCGTTGGTC ATCTCCCACA AGGATTTCGC TAGCCGAGAG 
GTCTATGATG CGGAACTTAC GCGAAATCTG AAGGCGGCTC GTGTGGATTT GATCTGCCTT 
GCCGGCTTCA TGCGTGTCCT GAGTGCTCCA TTCGTTCGAG AGTGGCGTGG ACGGCTCGTC 
AATATCCATC CCTCGCTGCT GCCCAAGTAT CCTGGTCTGC ATGTCCAGAA GCAGGCCTTG 
GAGGCGGGCG AAAAGGAGTC CGGCTGCACG GTTCACTTCG TGGACGAGGG TGTGGACACG 
GGAGCGATCA TTGTTCAGGC TGCTGTTCCC ATTTTGCCCG ATGACGACGA GGATTCGCTG 
ACCCAACGCA TCCACAAGGC TGAGCACTGG GCTTTTCCAA GGGCATTAGC AATGTTGGTC 
AACGGAACAG CTCTCATTTC TCCGGAAGTC AGCAGTCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285698.1
CDS	194..4255
Translation	MSHRVLVIGSGGREHAICWKLSQSPKVAQIYALPGSHGIQLVEKCRNLDAKTLDPKDFEAIAKWSKENQIALVVVGPEDPLALGLGDVLQSAGIPCFGPGKQGAQIEADKKWAKDFMLRHGIPTARYESFTDTEKAKAFIRSAPYPALVVKAAGLAAGKGVVVAANAKEACQAVDEILGDLKYGQAGATLVVEELLEGEEVSVLAFTDGKSVRAMLPAQDHKRLGNGDTGPNTGGMGAYCPCPLISQPALELVQKAVLERAVQGLIKERINYQGVLYAGLMLTRDGPRVLEFNCRFGDPETQVILPLLESDLFDVMEACCSGKLDKIPLQWRNGVSAVGVILASAGYPETSTKGCIISGLPAANTPTQLVFHSGLAVNAQKEALTNGGRVLIAIALDGSLKEAAAKATKLAGSISFSGSGAQYRTDIAQKAFKIASASTPGLSYKDSGVDIDAGDALVQRIKPLSRGTQRPGVIGGLGGFGGLFRLKELTYKEPVIAEATQGVGAKIHLALTHELYENVGYDLFALAANDVLEVGAEPVAFLDYIACGKLQVPLAAQLVKGMADGCRDARCALVGGETAEMPSLYAPGQHDMAGYCVGIVEQSRILPRFDLYQPGDLLIGLPSSGLHCAGFNEILTQLAASKVNLRERSPVDGGDDGLTLAHVLATPTQLYVQQLLPHLQKGDEIKSVAHVTHGLLNDILRLLPDGFETTLDFGAVPVPKIFGWLAGKLKLSAQTILERHNCGIGMVLILPQSSQLWRTSLPGAKVLGVLQRRSKVSGSPVQVRNFVEQLEKVASPFGGLGDRELPEELKKLPSNSDLSAPREECFENAAGRRLTRIPTHYKDPILILGTDGVGTKLKIAQQTNRNTSVGIDLVAMCVNDILCNGAEPISFSSYYACGHWQEQLAKEVHSGVQEGARQANSSFIDSHSAALPLLYEPQVYDLAGFALGIAEHTGILPLLDEIQPGDVLIGLPSSGVHSNGFSLVHAVLKRVGLGLHDKAPFSDKTLGEELLVPTKIYVKALSTLLSRGKHGIKALAHITGGGLSENIPRVLRKDLAVRLDANKFQLPPVFAWLAAAGNISSTELQRTYNCGLGMVLVVAPTEVEDVLKELRYPQRAAVVGEVVARKDPKKSQVVVQNFEASLARTQKMLSQRRKRVAVLISGTGSNLQALIDATRDSAQGIHADVVLVISNKTGVLGLQRATQAGIPSLVISHKDFASREVYDAELTRNLKAARVDLICLAGFMRVLSAPFVREWRGRLVNIHPSLLPKYPGLHVQKQALEAGEKESGCTVHFVDEGVDTGAIIVQAAVPILPDDDEDSLTQRIHKAEHWAFPRALAMLVNGTALISPEVSSQ

Target ORF information:

RefSeq Version	NM_001298769.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster GART trifunctional enzyme (Gart), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298769.1

ORF Insert Sequence:

ATGTCGCATC GCGTTCTGGT CATTGGCAGC GGCGGTCGGG AGCACGCGAT CTGCTGGAAG 
TTGTCGCAAT CTCCGAAGGT GGCCCAAATA TACGCACTGC CGGGTAGCCA TGGCATCCAG 
CTGGTGGAGA AGTGCCGGAA TCTGGATGCC AAGACCTTGG ATCCTAAGGA TTTCGAGGCC 
ATTGCTAAAT GGAGCAAGGA GAACCAGATA GCCCTAGTGG TTGTGGGACC CGAAGATCCC 
TTGGCTTTGG GTTTGGGCGA TGTGCTGCAG AGCGCTGGAA TTCCCTGCTT TGGACCCGGC 
AAGCAAGGTG CCCAAATCGA GGCGGACAAG AAGTGGGCCA AGGACTTTAT GCTGCGCCAT 
GGGATTCCCA CAGCGCGTTA CGAAAGTTTC ACAGACACCG AAAAGGCCAA GGCATTCATA 
AGAAGCGCTC CGTATCCAGC TCTGGTGGTG AAAGCTGCTG GATTGGCCGC CGGAAAGGGC 
GTGGTGGTGG CCGCCAATGC GAAGGAGGCC TGCCAGGCGG TGGACGAGAT CCTGGGAGAT 
CTGAAATACG GTCAGGCTGG CGCCACGCTG GTGGTGGAGG AGCTACTGGA GGGCGAAGAG 
GTTTCCGTTC TGGCCTTCAC CGATGGCAAA AGTGTGCGTG CCATGCTGCC AGCGCAGGAT 
CACAAGCGAC TGGGCAATGG CGATACTGGA CCAAACACCG GCGGCATGGG CGCCTACTGC 
CCTTGTCCGT TGATCAGCCA GCCAGCACTG GAACTGGTGC AAAAGGCCGT GCTGGAGCGG 
GCTGTGCAGG GTCTGATCAA GGAGAGGATC AACTACCAGG GTGTACTCTA TGCGGGACTC 
ATGCTGACAC GCGATGGTCC CCGTGTGCTG GAGTTTAACT GCCGGTTTGG AGATCCCGAG 
ACCCAGGTGA TTCTGCCGCT GCTCGAAAGC GATCTGTTCG ATGTGATGGA GGCTTGTTGC 
AGCGGAAAGC TGGACAAGAT TCCCCTGCAA TGGCGCAATG GAGTGAGTGC CGTTGGTGTG 
ATCCTGGCCA GTGCTGGCTA TCCGGAGACC TCCACAAAGG GTTGCATCAT ATCGGGACTT 
CCGGCTGCCA ATACACCCAC CCAACTGGTC TTTCACAGCG GCCTTGCCGT GAATGCACAA 
AAGGAAGCCC TAACCAATGG CGGTCGCGTC CTCATCGCGA TCGCTCTGGA CGGCAGCTTG 
AAGGAGGCTG CTGCGAAGGC AACCAAATTG GCTGGCAGCA TCAGTTTTTC GGGCTCTGGA 
GCTCAGTACC GAACGGACAT TGCCCAGAAG GCGTTCAAAA TTGCCAGTGC CAGCACCCCG 
GGGTTGAGTT ACAAGGACAG CGGAGTGGAC ATCGATGCTG GAGATGCACT GGTGCAACGC 
ATCAAGCCCT TGTCTCGGGG TACCCAGCGC CCGGGCGTGA TTGGCGGCTT GGGTGGATTT 
GGAGGACTGT TCCGTTTGAA GGAACTCACC TACAAGGAGC CAGTCATTGC TGAGGCCACT 
CAGGGAGTCG GAGCCAAGAT CCATCTTGCC CTGACGCACG AATTGTATGA AAATGTGGGC 
TACGACCTGT TTGCCTTGGC TGCTAATGAT GTCCTGGAAG TGGGTGCCGA GCCGGTCGCC 
TTTCTGGACT ACATTGCCTG TGGCAAGCTG CAAGTTCCAT TGGCCGCCCA GTTGGTCAAG 
GGAATGGCTG ATGGCTGCCG GGATGCGCGG TGCGCCTTAG TGGGTGGTGA GACTGCAGAG 
ATGCCTTCTC TATATGCTCC CGGTCAACAC GACATGGCTG GCTATTGTGT TGGCATAGTG 
GAGCAGTCTC GAATCCTGCC CCGTTTCGAT CTTTACCAGC CGGGTGACTT ACTCATCGGT 
CTGCCATCAT CTGGGCTTCA TTGCGCCGGC TTCAATGAAA TACTCACCCA GTTGGCGGCA 
TCCAAAGTGA ATCTCAGAGA GCGTTCTCCC GTGGACGGAG GCGACGATGG GCTGACCTTG 
GCCCATGTCT TAGCCACGCC CACCCAACTG TATGTCCAGC AGCTGTTGCC TCATCTTCAG 
AAAGGAGATG AGATCAAGTC GGTGGCTCAC GTGACCCATG GTCTGCTAAA CGATATCCTC 
CGTCTGCTTC CCGATGGATT TGAGACAACG CTGGATTTCG GTGCCGTCCC AGTGCCCAAA 
ATCTTTGGTT GGCTGGCCGG AAAGCTAAAG CTGAGTGCCC AAACAATTCT AGAGCGGCAC 
AACTGCGGCA TTGGCATGGT TCTGATCCTG CCTCAGTCAA GTCAGCTGTG GCGAACGTCC 
CTTCCGGGAG CCAAAGTACT GGGCGTCCTG CAACGGCGGT CAAAGGTTAG TGGATCACCT 
GTCCAGGTGC GCAACTTTGT GGAGCAACTG GAGAAGGTGG CCTCGCCGTT CGGTGGTCTC 
GGCGATCGGG AATTGCCCGA AGAGCTCAAG AAACTGCCTT CCAACTCGGA TTTATCAGCT 
CCTCGCGAGG AATGCTTTGA AAACGCAGCT GGACGTCGGC TCACGCGCAT ACCCACCCAC 
TACAAGGACC CCATACTCAT CCTGGGCACC GATGGAGTGG GCACCAAGCT GAAAATAGCG 
CAGCAAACGA ATCGCAACAC GAGCGTGGGT ATCGATTTGG TGGCCATGTG CGTCAACGAC 
ATCCTCTGCA ATGGAGCCGA GCCAATCAGT TTCTCAAGCT ACTATGCCTG CGGGCACTGG 
CAGGAGCAGT TGGCCAAGGA GGTTCACTCC GGGGTCCAAG AGGGTGCCAG GCAGGCGAAC 
AGCAGTTTCA TTGACTCACA TAGTGCCGCT CTGCCGCTCC TGTACGAACC CCAGGTCTAT 
GATCTGGCTG GTTTCGCCTT GGGCATAGCA GAACACACCG GAATTCTACC CCTTTTGGAT 
GAAATCCAGC CCGGAGATGT GCTCATCGGC TTACCTTCGT CTGGCGTCCA CAGCAACGGA 
TTTAGTTTGG TTCACGCCGT TTTGAAGCGA GTGGGCTTGG GTCTGCATGA CAAGGCGCCC 
TTTAGTGATA AGACGCTGGG CGAGGAGCTG CTGGTGCCCA CCAAGATCTA TGTCAAGGCA 
TTGTCCACTC TGCTGTCCCG CGGAAAACAC GGCATCAAGG CACTAGCCCA CATCACAGGA 
GGAGGCCTGA GCGAGAATAT ACCGCGAGTA TTGCGTAAGG ATTTGGCCGT GCGCCTGGAT 
GCCAATAAGT TCCAGCTTCC GCCCGTTTTC GCCTGGCTTG CAGCGGCCGG AAATATCAGC 
TCCACTGAGC TGCAGCGCAC ATATAACTGC GGCTTGGGGA TGGTTCTGGT GGTGGCCCCG 
ACGGAAGTCG AGGACGTTCT TAAGGAGCTG CGTTATCCGC AAAGAGCAGC TGTGGTCGGC 
GAGGTGGTGG CACGCAAGGA TCCGAAGAAG TCGCAGGTGG TGGTCCAGAA CTTTGAGGCA 
TCATTGGCCA GGACACAGAA GATGCTCTCG CAGCGTCGGA AACGAGTTGC TGTTCTCATT 
TCGGGAACGG GCAGCAACCT GCAAGCACTA ATTGATGCAA CGCGCGACTC GGCACAGGGT 
ATCCATGCCG ATGTTGTGCT GGTAATCAGC AATAAGACTG GTGTTCTGGG TCTGCAGCGA 
GCCACGCAAG CCGGAATACC TTCGTTGGTC ATCTCCCACA AGGATTTCGC TAGCCGAGAG 
GTCTATGATG CGGAACTTAC GCGAAATCTG AAGGCGGCTC GTGTGGATTT GATCTGCCTT 
GCCGGCTTCA TGCGTGTCCT GAGTGCTCCA TTCGTTCGAG AGTGGCGTGG ACGGCTCGTC 
AATATCCATC CCTCGCTGCT GCCCAAGTAT CCTGGTCTGC ATGTCCAGAA GCAGGCCTTG 
GAGGCGGGCG AAAAGGAGTC CGGCTGCACG GTTCACTTCG TGGACGAGGG TGTGGACACG 
GGAGCGATCA TTGTTCAGGC TGCTGTTCCC ATTTTGCCCG ATGACGACGA GGATTCGCTG 
ACCCAACGCA TCCACAAGGC TGAGCACTGG GCTTTTCCAA GGGCATTAGC AATGTTGGTC 
AACGGAACAG CTCTCATTTC TCCGGAAGTC AGCAGTCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Gart cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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